Endoribonuclease Dicer
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30016368 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 31
Homology
BLAST of Endoribonuclease Dicer vs. Ensembl Human
Match: DICER1 (dicer 1, ribonuclease III [Source:HGNC Symbol;Acc:HGNC:17098]) HSP 1 Score: 217.624 bits (553), Expect = 3.091e-56 Identity = 270/1063 (25.40%), Postives = 493/1063 (46.38%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDY-LNVKSD-----KKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVD-NWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIARE-FCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTL-KKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKS--DMLMCSEKCKKLINLILDLKS--------------KNED------------------------------FKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQ-YPKKLQMSV--TKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVF--THETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSERNVTPSEDDKYDYVPY----GENGPRITLNSASSVLNRYLGKLRCNSISE--PKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNR--------------FDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFL--KLGINDISMF---DVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFEYNLPVNSINY-SLLLKCHKILFNIYSERIRRFDKNIF-FDPSKAKSS---IILTILKDNK---IDFNKIKEIITNPI---VDNLIKNDQSSWV-------GSVIRPNYIT-NEFGAFVISKVCHDMNADSIFKND--MTYEKFLNKKYNIILSPNEKLSPLIKV------IRLTTIQSCVNKERRFLRNNRKLQRINESECY-----FVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDF 2884 R YQ+E+ A + N I+ LNTGSGKTFIA +L K+ ++ D K+ FLV +++ V Q ++++ + LK G+ N V+ +WTK++W+++ +K+++++MT V L++L + +LS + L+FDE H Y +I + S +ILGLTA ++ G +++E +++LE L T+ D + + + E +++ F D +++ + ++E + + ND + S+++++ + + K+ LS+ +VL LG + M + +L + ++ K L+ D FL + I++ E + S + D+ + K KL+ ++ K +N+D F N + +IF+ + + LN L+ K + +I+SNFI GH + P+ QM KQ E+L+ F+ + N+LIAT+++EEG+D+ CN+VV+FD P ++RSY+QSKGRARA S YI+ T + + E ++ + I KIL+++ + + D E ++ P DD + PY + GPR+T+N+A +NRY +L + + PK D + +L LP+ +P I G P S R A++ V L C+ LHK ELD L+P + + ++ + + Y K L + P YLY I + L +L ++ D + GIL PI Q FP+Y G ++ I+ + + + S+ L+ + H+ +F+ I R +K F P+ A S+ + L ++ D+ IDF +++I + + + ++ +V +VI P Y ++ F ++ V D+ S F + T+ ++ KYN+ L+ + PL+ V + L T + +N++ + L + +R + E VP L + +SLW + P+I +R++ +L+ E+ Sbjct: 45 RKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLK------VGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVD--CGPFTDRSGLYERLLMELEEAL----NFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFL---RKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAI----EKILRNKCSK---SVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPD--GTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPR---PDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQ---IPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFS----HILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQ--PLLDVDHTSSRLNLLTPRH-LNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEEL 1070 HSP 2 Score: 122.479 bits (306), Expect = 3.850e-27 Identity = 84/269 (31.23%), Postives = 135/269 (50.19%), Query Frame = 2 Query: 3281 NFVVK---NSDESVEVNNSREFI--------LIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVP-------------------------------------------DNELNADIEISPKLAFQNISMDTDIPQSIN---DLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE-ELLNNQLQCEHSYK 3913 +FVV S+E+ V+ ++ I I +K++ADC EA++G LT+ G + +L L L ++P D+E ++I P+ F + D + I+ + EK +NY FKNK Y++QA TH S N CYQRLEFLGD+++D+L+T ++ + ++ +PG +T+LRSALVNN FA L+VK+ +HKY ++FH++ V+ +L N++Q S K Sbjct: 1522 DFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGC-LKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSEK 1789 HSP 3 Score: 73.559 bits (179), Expect = 3.050e-12 Identity = 40/88 (45.45%), Postives = 56/88 (63.64%), Query Frame = 2 Query: 3047 NDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDES 3310 +D NLE+ E+LGDSFLK + YLF D +EG+L+ RS+ +SN NL+ L +KGL + F+P N+LPP +VV N D+S Sbjct: 1307 SDGFNLERLEMLGDSFLKHAITTYLFCTYPDA-HEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVV-NQDKS 1392
BLAST of Endoribonuclease Dicer vs. Ensembl Human
Match: DICER1 (dicer 1, ribonuclease III [Source:HGNC Symbol;Acc:HGNC:17098]) HSP 1 Score: 216.468 bits (550), Expect = 7.918e-56 Identity = 272/1068 (25.47%), Postives = 491/1068 (45.97%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDY-LNVKSD-----KKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVD-NWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIARE-FCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTL-KKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKS--DMLMCSEKCKKLINLILDLKS--------------KNED------------------------------FKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQ-YPKKLQMSV--TKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVF--THETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSERNVTPSEDDKYDYVPY----GENGPRITLNSASSVLNRYLGKLRCNSISE--PKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNR--------------FDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFL--KLGINDISMF---DVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFEYNLPVNSINY-SLLLKCHKILFNIYSERIRRFDKNIF-FDPSKAKSS---IILTILKDNK---IDFNKIKEIITN---------------PIVDNLIKNDQSSWVGSVIRPNYIT-NEFGAFVISKVCHDMNADSIFKND--MTYEKFLNKKYNIILSPNEKLSPLIKV------IRLTTIQSCVNKERRFLRNNRKLQRINESECY-----FVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDF 2884 R YQ+E+ A + N I+ LNTGSGKTFIA +L K+ ++ D K+ FLV +++ V Q ++++ + LK G+ N V+ +WTK++W+++ +K+++++MT V L++L + +LS + L+FDE H Y +I + S +ILGLTA ++ G +++E +++LE L T+ D + + + E +++ F D +++ + ++E + + ND + S+++++ + + K+ LS+ +VL LG + M + +L + ++ K L+ D FL + I++ E + S + D+ + K KL+ ++ K +N+D F N + +IF+ + + LN L+ K + +I+SNFI GH + P+ QM KQ E+L+ F+ + N+LIAT+++EEG+D+ CN+VV+FD P ++RSY+QSKGRARA S YI+ T + + E ++ + I KIL+++ + + D E ++ P DD + PY + GPR+T+N+A +NRY +L + + PK D + +L LP+ +P I G P S R A++ V L C+ LHK ELD L+P + + ++ + + Y K L + P YLY I + L +L ++ D + GIL PI Q FP+Y G ++ I+ + + + S+ L+ + H+ +F+ I R +K F P+ A S+ + L ++ D+ IDF +++I + P V L + +VI P Y ++ F ++ V D+ S F + T+ ++ KYN+ L+ + PL+ V + L T + +N++ + L + +R + E VP L + +SLW + P+I +R++ +L+ E+ Sbjct: 45 RKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLK------VGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVD--CGPFTDRSGLYERLLMELEEAL----NFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFL---RKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAI----EKILRNKCSK---SVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPD--GTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPR---PDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQ---IPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFS----HILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKL-----EDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQ--PLLDVDHTSSRLNLLTPRH-LNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEEL 1070 HSP 2 Score: 122.094 bits (305), Expect = 9.366e-39 Identity = 82/263 (31.18%), Postives = 133/263 (50.57%), Query Frame = 2 Query: 3281 NFVVK---NSDESVEVNNSREFI--------LIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVP-------------------------------------------DNELNADIEISPKLAFQNISMDTDIPQSIN---DLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE-ELLNNQLQ 3895 +FVV S+E+ V+ ++ I I +K++ADC EA++G LT+ G + +L L L ++P D+E ++I P+ F + D + I+ + EK +NY FKNK Y++QA TH S N CYQRLEFLGD+++D+L+T ++ + ++ +PG +T+LRSALVNN FA L+VK+ +HKY ++FH++ V+ +L N++Q Sbjct: 1522 DFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGC-LKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQ 1783 HSP 3 Score: 60.8474 bits (146), Expect = 9.366e-39 Identity = 28/60 (46.67%), Postives = 39/60 (65.00%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQ 4112 V K +GDIFES+AGAI++DS L+ VW VY M ++KF N+PR+ + L E P+ Sbjct: 1804 VPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPE 1863 HSP 4 Score: 73.559 bits (179), Expect = 2.903e-12 Identity = 40/88 (45.45%), Postives = 56/88 (63.64%), Query Frame = 2 Query: 3047 NDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDES 3310 +D NLE+ E+LGDSFLK + YLF D +EG+L+ RS+ +SN NL+ L +KGL + F+P N+LPP +VV N D+S Sbjct: 1307 SDGFNLERLEMLGDSFLKHAITTYLFCTYPDA-HEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVV-NQDKS 1392
BLAST of Endoribonuclease Dicer vs. Ensembl Human
Match: DICER1 (dicer 1, ribonuclease III [Source:HGNC Symbol;Acc:HGNC:17098]) HSP 1 Score: 216.468 bits (550), Expect = 7.918e-56 Identity = 272/1068 (25.47%), Postives = 491/1068 (45.97%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDY-LNVKSD-----KKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVD-NWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIARE-FCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTL-KKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKS--DMLMCSEKCKKLINLILDLKS--------------KNED------------------------------FKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQ-YPKKLQMSV--TKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVF--THETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSERNVTPSEDDKYDYVPY----GENGPRITLNSASSVLNRYLGKLRCNSISE--PKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNR--------------FDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFL--KLGINDISMF---DVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFEYNLPVNSINY-SLLLKCHKILFNIYSERIRRFDKNIF-FDPSKAKSS---IILTILKDNK---IDFNKIKEIITN---------------PIVDNLIKNDQSSWVGSVIRPNYIT-NEFGAFVISKVCHDMNADSIFKND--MTYEKFLNKKYNIILSPNEKLSPLIKV------IRLTTIQSCVNKERRFLRNNRKLQRINESECY-----FVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDF 2884 R YQ+E+ A + N I+ LNTGSGKTFIA +L K+ ++ D K+ FLV +++ V Q ++++ + LK G+ N V+ +WTK++W+++ +K+++++MT V L++L + +LS + L+FDE H Y +I + S +ILGLTA ++ G +++E +++LE L T+ D + + + E +++ F D +++ + ++E + + ND + S+++++ + + K+ LS+ +VL LG + M + +L + ++ K L+ D FL + I++ E + S + D+ + K KL+ ++ K +N+D F N + +IF+ + + LN L+ K + +I+SNFI GH + P+ QM KQ E+L+ F+ + N+LIAT+++EEG+D+ CN+VV+FD P ++RSY+QSKGRARA S YI+ T + + E ++ + I KIL+++ + + D E ++ P DD + PY + GPR+T+N+A +NRY +L + + PK D + +L LP+ +P I G P S R A++ V L C+ LHK ELD L+P + + ++ + + Y K L + P YLY I + L +L ++ D + GIL PI Q FP+Y G ++ I+ + + + S+ L+ + H+ +F+ I R +K F P+ A S+ + L ++ D+ IDF +++I + P V L + +VI P Y ++ F ++ V D+ S F + T+ ++ KYN+ L+ + PL+ V + L T + +N++ + L + +R + E VP L + +SLW + P+I +R++ +L+ E+ Sbjct: 45 RKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLK------VGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVD--CGPFTDRSGLYERLLMELEEAL----NFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFL---RKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAI----EKILRNKCSK---SVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPD--GTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPR---PDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQ---IPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFS----HILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKL-----EDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQ--PLLDVDHTSSRLNLLTPRH-LNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEEL 1070 HSP 2 Score: 122.094 bits (305), Expect = 9.366e-39 Identity = 82/263 (31.18%), Postives = 133/263 (50.57%), Query Frame = 2 Query: 3281 NFVVK---NSDESVEVNNSREFI--------LIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVP-------------------------------------------DNELNADIEISPKLAFQNISMDTDIPQSIN---DLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE-ELLNNQLQ 3895 +FVV S+E+ V+ ++ I I +K++ADC EA++G LT+ G + +L L L ++P D+E ++I P+ F + D + I+ + EK +NY FKNK Y++QA TH S N CYQRLEFLGD+++D+L+T ++ + ++ +PG +T+LRSALVNN FA L+VK+ +HKY ++FH++ V+ +L N++Q Sbjct: 1522 DFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGC-LKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQ 1783 HSP 3 Score: 60.8474 bits (146), Expect = 9.366e-39 Identity = 28/60 (46.67%), Postives = 39/60 (65.00%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQ 4112 V K +GDIFES+AGAI++DS L+ VW VY M ++KF N+PR+ + L E P+ Sbjct: 1804 VPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPE 1863 HSP 4 Score: 73.559 bits (179), Expect = 2.903e-12 Identity = 40/88 (45.45%), Postives = 56/88 (63.64%), Query Frame = 2 Query: 3047 NDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDES 3310 +D NLE+ E+LGDSFLK + YLF D +EG+L+ RS+ +SN NL+ L +KGL + F+P N+LPP +VV N D+S Sbjct: 1307 SDGFNLERLEMLGDSFLKHAITTYLFCTYPDA-HEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVV-NQDKS 1392
BLAST of Endoribonuclease Dicer vs. Ensembl Human
Match: DICER1 (dicer 1, ribonuclease III [Source:HGNC Symbol;Acc:HGNC:17098]) HSP 1 Score: 216.468 bits (550), Expect = 7.918e-56 Identity = 272/1068 (25.47%), Postives = 491/1068 (45.97%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDY-LNVKSD-----KKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVD-NWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIARE-FCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTL-KKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKS--DMLMCSEKCKKLINLILDLKS--------------KNED------------------------------FKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQ-YPKKLQMSV--TKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVF--THETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSERNVTPSEDDKYDYVPY----GENGPRITLNSASSVLNRYLGKLRCNSISE--PKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNR--------------FDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFL--KLGINDISMF---DVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFEYNLPVNSINY-SLLLKCHKILFNIYSERIRRFDKNIF-FDPSKAKSS---IILTILKDNK---IDFNKIKEIITN---------------PIVDNLIKNDQSSWVGSVIRPNYIT-NEFGAFVISKVCHDMNADSIFKND--MTYEKFLNKKYNIILSPNEKLSPLIKV------IRLTTIQSCVNKERRFLRNNRKLQRINESECY-----FVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDF 2884 R YQ+E+ A + N I+ LNTGSGKTFIA +L K+ ++ D K+ FLV +++ V Q ++++ + LK G+ N V+ +WTK++W+++ +K+++++MT V L++L + +LS + L+FDE H Y +I + S +ILGLTA ++ G +++E +++LE L T+ D + + + E +++ F D +++ + ++E + + ND + S+++++ + + K+ LS+ +VL LG + M + +L + ++ K L+ D FL + I++ E + S + D+ + K KL+ ++ K +N+D F N + +IF+ + + LN L+ K + +I+SNFI GH + P+ QM KQ E+L+ F+ + N+LIAT+++EEG+D+ CN+VV+FD P ++RSY+QSKGRARA S YI+ T + + E ++ + I KIL+++ + + D E ++ P DD + PY + GPR+T+N+A +NRY +L + + PK D + +L LP+ +P I G P S R A++ V L C+ LHK ELD L+P + + ++ + + Y K L + P YLY I + L +L ++ D + GIL PI Q FP+Y G ++ I+ + + + S+ L+ + H+ +F+ I R +K F P+ A S+ + L ++ D+ IDF +++I + P V L + +VI P Y ++ F ++ V D+ S F + T+ ++ KYN+ L+ + PL+ V + L T + +N++ + L + +R + E VP L + +SLW + P+I +R++ +L+ E+ Sbjct: 45 RKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLK------VGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVD--CGPFTDRSGLYERLLMELEEAL----NFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFL---RKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAI----EKILRNKCSK---SVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPD--GTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPR---PDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQ---IPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFS----HILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKL-----EDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQ--PLLDVDHTSSRLNLLTPRH-LNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEEL 1070 HSP 2 Score: 122.094 bits (305), Expect = 9.366e-39 Identity = 82/263 (31.18%), Postives = 133/263 (50.57%), Query Frame = 2 Query: 3281 NFVVK---NSDESVEVNNSREFI--------LIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVP-------------------------------------------DNELNADIEISPKLAFQNISMDTDIPQSIN---DLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE-ELLNNQLQ 3895 +FVV S+E+ V+ ++ I I +K++ADC EA++G LT+ G + +L L L ++P D+E ++I P+ F + D + I+ + EK +NY FKNK Y++QA TH S N CYQRLEFLGD+++D+L+T ++ + ++ +PG +T+LRSALVNN FA L+VK+ +HKY ++FH++ V+ +L N++Q Sbjct: 1522 DFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGC-LKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQ 1783 HSP 3 Score: 60.8474 bits (146), Expect = 9.366e-39 Identity = 28/60 (46.67%), Postives = 39/60 (65.00%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQ 4112 V K +GDIFES+AGAI++DS L+ VW VY M ++KF N+PR+ + L E P+ Sbjct: 1804 VPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPE 1863 HSP 4 Score: 73.559 bits (179), Expect = 2.903e-12 Identity = 40/88 (45.45%), Postives = 56/88 (63.64%), Query Frame = 2 Query: 3047 NDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDES 3310 +D NLE+ E+LGDSFLK + YLF D +EG+L+ RS+ +SN NL+ L +KGL + F+P N+LPP +VV N D+S Sbjct: 1307 SDGFNLERLEMLGDSFLKHAITTYLFCTYPDA-HEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVV-NQDKS 1392
BLAST of Endoribonuclease Dicer vs. Ensembl Human
Match: DICER1 (dicer 1, ribonuclease III [Source:HGNC Symbol;Acc:HGNC:17098]) HSP 1 Score: 216.468 bits (550), Expect = 7.918e-56 Identity = 272/1068 (25.47%), Postives = 491/1068 (45.97%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDY-LNVKSD-----KKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVD-NWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIARE-FCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTL-KKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKS--DMLMCSEKCKKLINLILDLKS--------------KNED------------------------------FKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQ-YPKKLQMSV--TKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVF--THETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSERNVTPSEDDKYDYVPY----GENGPRITLNSASSVLNRYLGKLRCNSISE--PKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNR--------------FDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFL--KLGINDISMF---DVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFEYNLPVNSINY-SLLLKCHKILFNIYSERIRRFDKNIF-FDPSKAKSS---IILTILKDNK---IDFNKIKEIITN---------------PIVDNLIKNDQSSWVGSVIRPNYIT-NEFGAFVISKVCHDMNADSIFKND--MTYEKFLNKKYNIILSPNEKLSPLIKV------IRLTTIQSCVNKERRFLRNNRKLQRINESECY-----FVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDF 2884 R YQ+E+ A + N I+ LNTGSGKTFIA +L K+ ++ D K+ FLV +++ V Q ++++ + LK G+ N V+ +WTK++W+++ +K+++++MT V L++L + +LS + L+FDE H Y +I + S +ILGLTA ++ G +++E +++LE L T+ D + + + E +++ F D +++ + ++E + + ND + S+++++ + + K+ LS+ +VL LG + M + +L + ++ K L+ D FL + I++ E + S + D+ + K KL+ ++ K +N+D F N + +IF+ + + LN L+ K + +I+SNFI GH + P+ QM KQ E+L+ F+ + N+LIAT+++EEG+D+ CN+VV+FD P ++RSY+QSKGRARA S YI+ T + + E ++ + I KIL+++ + + D E ++ P DD + PY + GPR+T+N+A +NRY +L + + PK D + +L LP+ +P I G P S R A++ V L C+ LHK ELD L+P + + ++ + + Y K L + P YLY I + L +L ++ D + GIL PI Q FP+Y G ++ I+ + + + S+ L+ + H+ +F+ I R +K F P+ A S+ + L ++ D+ IDF +++I + P V L + +VI P Y ++ F ++ V D+ S F + T+ ++ KYN+ L+ + PL+ V + L T + +N++ + L + +R + E VP L + +SLW + P+I +R++ +L+ E+ Sbjct: 45 RKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLK------VGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVD--CGPFTDRSGLYERLLMELEEAL----NFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFL---RKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAI----EKILRNKCSK---SVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPD--GTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPR---PDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQ---IPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFS----HILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKL-----EDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQ--PLLDVDHTSSRLNLLTPRH-LNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEEL 1070 HSP 2 Score: 122.094 bits (305), Expect = 9.366e-39 Identity = 82/263 (31.18%), Postives = 133/263 (50.57%), Query Frame = 2 Query: 3281 NFVVK---NSDESVEVNNSREFI--------LIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVP-------------------------------------------DNELNADIEISPKLAFQNISMDTDIPQSIN---DLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE-ELLNNQLQ 3895 +FVV S+E+ V+ ++ I I +K++ADC EA++G LT+ G + +L L L ++P D+E ++I P+ F + D + I+ + EK +NY FKNK Y++QA TH S N CYQRLEFLGD+++D+L+T ++ + ++ +PG +T+LRSALVNN FA L+VK+ +HKY ++FH++ V+ +L N++Q Sbjct: 1522 DFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGC-LKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQ 1783 HSP 3 Score: 60.8474 bits (146), Expect = 9.366e-39 Identity = 28/60 (46.67%), Postives = 39/60 (65.00%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQ 4112 V K +GDIFES+AGAI++DS L+ VW VY M ++KF N+PR+ + L E P+ Sbjct: 1804 VPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPE 1863 HSP 4 Score: 73.559 bits (179), Expect = 2.903e-12 Identity = 40/88 (45.45%), Postives = 56/88 (63.64%), Query Frame = 2 Query: 3047 NDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDES 3310 +D NLE+ E+LGDSFLK + YLF D +EG+L+ RS+ +SN NL+ L +KGL + F+P N+LPP +VV N D+S Sbjct: 1307 SDGFNLERLEMLGDSFLKHAITTYLFCTYPDA-HEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVV-NQDKS 1392
BLAST of Endoribonuclease Dicer vs. Ensembl Celegans
Match: dcr-1 (Endoribonuclease dcr-1 Death-promoting deoxyribonuclease [Source:UniProtKB/Swiss-Prot;Acc:P34529]) HSP 1 Score: 189.119 bits (479), Expect = 6.052e-48 Identity = 186/685 (27.15%), Postives = 334/685 (48.76%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDY-------LNVKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHH-LTKSDDYSQI--SEHVIRTTSDI-KILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIA-REFCGNIKETII-------------NYET--ESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKSDMLMCSEK--CKKLINLILDLKSKNEDFKNT---------ISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQYPKKLQMSVTK-QXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHE--TQKTEAQIQKFNLINEELNKIL--KSRVIEDELNKDDSERNVTPSEDDKYDYVPYGENGPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYF----------QDEFNFHHVSLLLPMPAPYLEEIK-GLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNR 1939 RDYQ+E+ +KA +N I++L TGSGKTFIA +L+K+Y L+ + K+ FF+V+ +LV Q ++ + K +G +GQ + D +K++ + + ++ ++V+TA+ LD++ H + +C LIFDE HH L Y I +++ + ++LGLTA LIK + + +K+LE ++ + T+ D ++ ++ E +I N++T E F + VA + E F P + + + + +K L + +V LG ++ ++L + S +KT +L+ + + I +E + K + +++I L L++ N +F+ +S +IF++++ +L +++ + F F+N ++++G S + K Q E+L+ F + N LIAT+V+EEG+DV CN+V+KFD P D RSY+QSKGRAR S+Y++ E T ++ ++ F +++ KIL + R + + + +DDS+R D + + + G + +++A +++NRY KL P I+T ++ + LLLP+ +P I P NK+ A+ V LEAC+ LH ELD +LLP R Sbjct: 14 RDYQVELLDKATKKNTIVQLGTGSGKTFIAVLLLKEYGVQLFAPLD-QGGKRAFFVVEKVNLVEQQAIHIEVHTSFK--VGQVHGQTSSGLWD--SKEQCDQFMKRHHVVVITAQCLLDLIRHAYLKIEDMCVLIFDECHHALGSQHPYRSIMVDYKLLKKDKPVPRVLGLTASLIKAKVAPEKLMEQLKKLESAMDSVIETASDLVSLSKYGAKPYEVVIICKDFEIGCLGIPNFDTVIEIFDETVAFVNTTTE----FHP-------------DLDLDPRRPIKDSLKTTRAVFRQLGPWAAWRTAQVWEKELGKIIK--SQVLPDKTLRFLNMAKTSMITIKRLLEPEMKKIKSIEALRPYVPQRVIRLFEILETFNPEFQKERMKLEKAEHLSAIIFVDQRYIAYSLLLMMRHIKSWEPKFKFVNPDYVVGASGRNLASSDSQGLHKRQTEVLRRFHRNEINCLIATSVLEEGVDVKQCNLVIKFDRPLDMRSYVQSKGRARRAGSRYVITVEEKDTAACDSDLKDF----QQIEKILLSRHRTVNNPI-EDDSDRFEEFDVDSQMEPYVVEKTGATLKMSTAIALINRYCSKL-------PSDIFTRLVPHNQIIPIEENGVTKYCAELLLPINSPIKHAIVLKNPMPNKKTAQMAVALEACRQLHLEGELDDNLLPKGR 662 HSP 2 Score: 157.532 bits (397), Expect = 1.565e-46 Identity = 116/366 (31.69%), Postives = 179/366 (48.91%), Query Frame = 2 Query: 3014 NIFPCFM-----AKTCNDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPP------NFVVKNSDESVEVNNSREFILIK-------------------NKNLADCCEAI-------IG---------SILTNYGI-DYSI---------------------QYLQWLNLPLVPDNELNADIEISPKLAFQNISMDTDIPQSIN------------DLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE 3871 + PC + D +LE+FE +GDSFLKF T+ YL+H D +EGKL+ RS+ +SN NL+ L + G+ + F+ ++LPP +F N+D++ E +N E IL +K+ D E I +G ++LT I D SI + + W+ L ++ ++ +D+ SP L F I T+ S+N LE+ + Y FK + Y+VQA TH S I NR CYQRLEFLGD+V+D+++T Y+ ++++ +PG +T+LRSALVNN FA L+VKF F K+ ++H++ K V+ Sbjct: 1391 GVSPCLLLTALTTSNAADGMSLERFETIGDSFLKFATTDYLYHTLLD-QHEGKLSFARSKEVSNCNLYRLGKKLGIPQLIVANKFDAHDSWLPPCYIPTCDFKAPNTDDAEEKDNEIERILDGQVIEEKPENKTGWDIGGDVSKSTTDGIETITFPKQARVGNDDISPLPYNLLTQQHISDKSIADAVEALIGVHLLTLGPNPTLKVMNWMGLKVIQKDQ-KSDVP-SPLLRF--IDTPTNPNASLNFLNNLWQQFQFTQLEEKIGYRFKERAYLVQAFTHASYINNRVTGCYQRLEFLGDAVLDYMITRYLFEDSRQYSPGVLTDLRSALVNNTIFASLAVKFEFQKHFIAMCPGLYHMIEKFVK 1751 HSP 3 Score: 50.447 bits (119), Expect = 1.565e-46 Identity = 35/104 (33.65%), Postives = 50/104 (48.08%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQMKCTFENQEF---SNLIKLTAILPNGSVIEKYERNKILCKLSLA 4235 V K +GDIFES+AGAI++DS +L W V M ++ N PR+ I L E + Q K F E S +++T + N RN + K + A Sbjct: 1785 VPKAMGDIFESVAGAIYLDSGRNLDTTWQVIFHMMRGTIELCCANPPRSPIRELME-FEQSKVRFSKMERILESGKVRVTVEVVNNMRFTGMGRNYRIAKATAA 1887
BLAST of Endoribonuclease Dicer vs. Ensembl Celegans
Match: dcr-1 (Endoribonuclease dcr-1 Death-promoting deoxyribonuclease [Source:UniProtKB/Swiss-Prot;Acc:P34529]) HSP 1 Score: 189.119 bits (479), Expect = 6.052e-48 Identity = 186/685 (27.15%), Postives = 334/685 (48.76%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDY-------LNVKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHH-LTKSDDYSQI--SEHVIRTTSDI-KILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIA-REFCGNIKETII-------------NYET--ESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKSDMLMCSEK--CKKLINLILDLKSKNEDFKNT---------ISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQYPKKLQMSVTK-QXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHE--TQKTEAQIQKFNLINEELNKIL--KSRVIEDELNKDDSERNVTPSEDDKYDYVPYGENGPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYF----------QDEFNFHHVSLLLPMPAPYLEEIK-GLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNR 1939 RDYQ+E+ +KA +N I++L TGSGKTFIA +L+K+Y L+ + K+ FF+V+ +LV Q ++ + K +G +GQ + D +K++ + + ++ ++V+TA+ LD++ H + +C LIFDE HH L Y I +++ + ++LGLTA LIK + + +K+LE ++ + T+ D ++ ++ E +I N++T E F + VA + E F P + + + + +K L + +V LG ++ ++L + S +KT +L+ + + I +E + K + +++I L L++ N +F+ +S +IF++++ +L +++ + F F+N ++++G S + K Q E+L+ F + N LIAT+V+EEG+DV CN+V+KFD P D RSY+QSKGRAR S+Y++ E T ++ ++ F +++ KIL + R + + + +DDS+R D + + + G + +++A +++NRY KL P I+T ++ + LLLP+ +P I P NK+ A+ V LEAC+ LH ELD +LLP R Sbjct: 14 RDYQVELLDKATKKNTIVQLGTGSGKTFIAVLLLKEYGVQLFAPLD-QGGKRAFFVVEKVNLVEQQAIHIEVHTSFK--VGQVHGQTSSGLWD--SKEQCDQFMKRHHVVVITAQCLLDLIRHAYLKIEDMCVLIFDECHHALGSQHPYRSIMVDYKLLKKDKPVPRVLGLTASLIKAKVAPEKLMEQLKKLESAMDSVIETASDLVSLSKYGAKPYEVVIICKDFEIGCLGIPNFDTVIEIFDETVAFVNTTTE----FHP-------------DLDLDPRRPIKDSLKTTRAVFRQLGPWAAWRTAQVWEKELGKIIK--SQVLPDKTLRFLNMAKTSMITIKRLLEPEMKKIKSIEALRPYVPQRVIRLFEILETFNPEFQKERMKLEKAEHLSAIIFVDQRYIAYSLLLMMRHIKSWEPKFKFVNPDYVVGASGRNLASSDSQGLHKRQTEVLRRFHRNEINCLIATSVLEEGVDVKQCNLVIKFDRPLDMRSYVQSKGRARRAGSRYVITVEEKDTAACDSDLKDF----QQIEKILLSRHRTVNNPI-EDDSDRFEEFDVDSQMEPYVVEKTGATLKMSTAIALINRYCSKL-------PSDIFTRLVPHNQIIPIEENGVTKYCAELLLPINSPIKHAIVLKNPMPNKKTAQMAVALEACRQLHLEGELDDNLLPKGR 662 HSP 2 Score: 157.532 bits (397), Expect = 1.565e-46 Identity = 116/366 (31.69%), Postives = 179/366 (48.91%), Query Frame = 2 Query: 3014 NIFPCFM-----AKTCNDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPP------NFVVKNSDESVEVNNSREFILIK-------------------NKNLADCCEAI-------IG---------SILTNYGI-DYSI---------------------QYLQWLNLPLVPDNELNADIEISPKLAFQNISMDTDIPQSIN------------DLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE 3871 + PC + D +LE+FE +GDSFLKF T+ YL+H D +EGKL+ RS+ +SN NL+ L + G+ + F+ ++LPP +F N+D++ E +N E IL +K+ D E I +G ++LT I D SI + + W+ L ++ ++ +D+ SP L F I T+ S+N LE+ + Y FK + Y+VQA TH S I NR CYQRLEFLGD+V+D+++T Y+ ++++ +PG +T+LRSALVNN FA L+VKF F K+ ++H++ K V+ Sbjct: 1391 GVSPCLLLTALTTSNAADGMSLERFETIGDSFLKFATTDYLYHTLLD-QHEGKLSFARSKEVSNCNLYRLGKKLGIPQLIVANKFDAHDSWLPPCYIPTCDFKAPNTDDAEEKDNEIERILDGQVIEEKPENKTGWDIGGDVSKSTTDGIETITFPKQARVGNDDISPLPYNLLTQQHISDKSIADAVEALIGVHLLTLGPNPTLKVMNWMGLKVIQKDQ-KSDVP-SPLLRF--IDTPTNPNASLNFLNNLWQQFQFTQLEEKIGYRFKERAYLVQAFTHASYINNRVTGCYQRLEFLGDAVLDYMITRYLFEDSRQYSPGVLTDLRSALVNNTIFASLAVKFEFQKHFIAMCPGLYHMIEKFVK 1751 HSP 3 Score: 50.447 bits (119), Expect = 1.565e-46 Identity = 35/104 (33.65%), Postives = 50/104 (48.08%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQMKCTFENQEF---SNLIKLTAILPNGSVIEKYERNKILCKLSLA 4235 V K +GDIFES+AGAI++DS +L W V M ++ N PR+ I L E + Q K F E S +++T + N RN + K + A Sbjct: 1785 VPKAMGDIFESVAGAIYLDSGRNLDTTWQVIFHMMRGTIELCCANPPRSPIRELME-FEQSKVRFSKMERILESGKVRVTVEVVNNMRFTGMGRNYRIAKATAA 1887
BLAST of Endoribonuclease Dicer vs. Ensembl Celegans
Match: drsh-1 (Ribonuclease 3 [Source:UniProtKB/Swiss-Prot;Acc:O01326]) HSP 1 Score: 62.003 bits (149), Expect = 3.536e-9 Identity = 65/266 (24.44%), Postives = 122/266 (45.86%), Query Frame = 2 Query: 3065 EQFEILGDSFLKFITSKYLF-----HFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGI---DYSIQYLQWLNLPLVPD--NELNA-DIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANRKFSCY--QRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYL 3823 E+ E LGD+ ++ I S +L+ HF EG L R+ L+ N+NL LA +D+ Y++ ++ N EF LA+ EA++ +I + G+ D + + P++ + + +N +++ +++S T + + LE+ L F N + +A T +I N + QRLE+LGDSV+ +V+ ++ + G ++ LR++LV+N T A + GF +++ Sbjct: 691 ERLEYLGDAVVELIVSHHLYFMLTHHF------EGGLATYRTALVQNRNLATLAKNCRIDEMLQYSH-------------------GADLINVAEF----KHALANAFEAVMAAIYLDGGLAPCDVIFSKAMYGHQPVLKEKWDHINEHELKREDPQGDRDLSFITPTLSTFHALEERLGIQFNNIRLLAKAFTR-RNIPNNDLTKGHNQRLEWLGDSVLQLIVSDFLYRRFPYHHEGHMSLLRTSLVSNQTQAVVCDDLGFTEFV 926
BLAST of Endoribonuclease Dicer vs. Ensembl Celegans
Match: drsh-1 (Ribonuclease 3 [Source:UniProtKB/Swiss-Prot;Acc:O01326]) HSP 1 Score: 62.003 bits (149), Expect = 3.653e-9 Identity = 65/266 (24.44%), Postives = 122/266 (45.86%), Query Frame = 2 Query: 3065 EQFEILGDSFLKFITSKYLF-----HFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGI---DYSIQYLQWLNLPLVPD--NELNA-DIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANRKFSCY--QRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYL 3823 E+ E LGD+ ++ I S +L+ HF EG L R+ L+ N+NL LA +D+ Y++ ++ N EF LA+ EA++ +I + G+ D + + P++ + + +N +++ +++S T + + LE+ L F N + +A T +I N + QRLE+LGDSV+ +V+ ++ + G ++ LR++LV+N T A + GF +++ Sbjct: 689 ERLEYLGDAVVELIVSHHLYFMLTHHF------EGGLATYRTALVQNRNLATLAKNCRIDEMLQYSH-------------------GADLINVAEF----KHALANAFEAVMAAIYLDGGLAPCDVIFSKAMYGHQPVLKEKWDHINEHELKREDPQGDRDLSFITPTLSTFHALEERLGIQFNNIRLLAKAFTR-RNIPNNDLTKGHNQRLEWLGDSVLQLIVSDFLYRRFPYHHEGHMSLLRTSLVSNQTQAVVCDDLGFTEFV 924
BLAST of Endoribonuclease Dicer vs. Ensembl Celegans
Match: drh-1 (Dicer Related Helicase [Source:UniProtKB/TrEMBL;Acc:V6CIX1]) HSP 1 Score: 53.1434 bits (126), Expect = 1.678e-6 Identity = 52/195 (26.67%), Postives = 92/195 (47.18%), Query Frame = 2 Query: 47 RDYQIEIYNKA-KNENVIIKLNTGSGKTFIASMLIKDYLNVKSDK----KIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNK-----------YNLSQVCYLIFDEVHHLTKSDDYSQISE--HVIRTTSDI-------KILGLTACLIKGVKH 556 R+YQ E+ A + +N I+ TGSGKT IA+ +IK++ +S + K F+ NS +++Q S+ YL D + ++ Q+ + I +IV T ++ +++ N ++ + LS + FDE H+ K+ YS I H ++ ++ +I+GLTA L G K+ Sbjct: 36 RNYQEELCQVALQGKNTIVTAPTGSGKTVIAANIIKEHFESRSSEGKRFKALFMTPNSMILNQQAASISSYL---DHV--YHTQI----IQGSDNVPTRNVIQSKDLIVATPQMIVNLCNEHRNSLDDESRLDQFFLSTFTIIFFDECHNTVKNSPYSNIMREYHYLKNMGNMPEGHSLPQIIGLTASLGTGDKN 221
BLAST of Endoribonuclease Dicer vs. Ensembl Fly
Match: Dcr-1 (gene:FBgn0039016 transcript:FBtr0084324) HSP 1 Score: 125.561 bits (314), Expect = 4.164e-41 Identity = 68/203 (33.50%), Postives = 113/203 (55.67%), Query Frame = 2 Query: 3344 IKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVP----------------DNELNAD----------IEISPKLAFQ-NISMDTD-IPQSINDLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVV 3868 I +K++ADC EA+IG+ L G ++ ++ WL + ++P + NA+ SP L F N + + D + + E+ L Y F+++ Y++QA+TH S NR CYQRLEFLGD+V+D+L+T ++ + ++ +PG +T+LRSALVNN FA L+V+ GFHK+ + + ++ + V Sbjct: 1898 IPDKSIADCVEALIGAYLIECGPRGALLFMAWLGVRVLPITRQLDGGNQEQRIPGSTKPNAENVVTVYGAWPTPRSPLLHFAPNATEELDQLLSGFEEFEESLGYKFRDRSYLLQAMTHASYTPNRLTDCYQRLEFLGDAVLDYLITRHLYEDPRQHSPGALTDLRSALVNNTIFASLAVRHGFHKFFRHLSPGLNDVIDRFV 2100 HSP 2 Score: 64.6994 bits (156), Expect = 4.164e-41 Identity = 30/60 (50.00%), Postives = 40/60 (66.67%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQ 4112 V K LGD+FESIAGAIF+DSN L VW VY M +++F N++P++ I L E P+ Sbjct: 2130 VPKALGDVFESIAGAIFLDSNMSLDVVWHVYSNMMSPEIEQFSNSVPKSPIRELLELEPE 2189 HSP 3 Score: 71.633 bits (174), Expect = 6.245e-12 Identity = 36/92 (39.13%), Postives = 53/92 (57.61%), Query Frame = 2 Query: 3017 IFPCFMAKTCNDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVV 3292 I ND NLE+ E +GDSFLK+ + YL+ + + +EGKL+ RS+ ++N NL+ L +K L +Y T F P N+LPP + V Sbjct: 1726 ILQALTMSNANDGINLERLETIGDSFLKYAITTYLY-ITYENVHEGKLSHLRSKQVANLNLYRLGRRKRLGEYMIATKFEPHDNWLPPCYYV 1816 HSP 4 Score: 71.2478 bits (173), Expect = 8.972e-12 Identity = 45/134 (33.58%), Postives = 78/134 (58.21%), Query Frame = 2 Query: 1103 DFKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFI---IGHSTSQYPKKLQMSVTKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHETQKT 1495 D +T+ LI+ N+ L LL + + + F+ + + T++ PK+ ++ +Q E+LK F+ N+LI T+V+EEG+DV CN+VV++DPP +RSY+Q KGRARA + +++ + K+ Sbjct: 488 DGSDTLCALIYCNQNHTARVLFELLAEISRRDPDLKFLRCQYTTDRVADPTTE-PKEAELEHRRQEEVLKRFRMHDCNVLIGTSVLEEGIDVPKCNLVVRWDPPTTYRSYVQCKGRARAAPAYHVILVAPSYKS 620 HSP 5 Score: 58.5362 bits (140), Expect = 6.545e-8 Identity = 99/474 (20.89%), Postives = 186/474 (39.24%), Query Frame = 2 Query: 1625 YDYVPYGENGPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYFQDE---FNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNR----------------FDNNLVIQESNDIE--IIKYEKYTATKLSTKFQNL-SIPANKLYLYSIKYT-----PSVFLKLGINDISMFDVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFEYNLPVNSINYSLLLKCHKILFNIYSERIRRFDKNI-FFDPSKAKSSI-ILTILK----DNKIDFNKIKEIITN--------PIVDNLIKN-DQSSWVGSVIRPNYI-TNEFGAFVISKVCHDMNADSIFKND--MTYEKFLNKKYNIILSPNEKLSPLIKVIRLTT-----IQSCVNKERRFL----RNNRKLQRIN-ESECYFVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIED 2881 Y P+ G + L SA +++N+Y +L ++ ++ ++ ++E +L LP+ +P +I GLP + A++ L+AC LH+ ELD L P + ++ ++Q S++ K +Y +++++F + + YLY I+ T P G D GIL I + F I+ G V ++ I S + C N + R K + FDP ++ + I+ +K ID+ ++ I N P + + D + +V+ P Y ++ F ++++C ++ S F D T++ + KY + + + PL+ V + VN++ L ++ +R N E + VP L +SLW P I +R+N +L +D Sbjct: 808 YRPKPHLLTGASVDLGSAIALVNKYCARLPSDTFTKLTALWRCTRNERAGVTLFQYTLRLPINSPLKHDIVGLPMPTQTLARRLAALQACVELHRIGELDDQLQPIGKEGFRALEPDWECFELEPEDEQIVQLSDEPRPGTTKRRQYYYKRIASEFCDCRPVAGAPCYLYFIQLTLQCPIPEEQNTRGRKIYPPEDAQQGFGILTTKRIPK---LSAFSIFTRSGEVKVSLELAKE---RVILTSEQIVCINGFLNYTFTNVLRLQKFLMLFDPDSTENCVFIVPTVKAPAGGKHIDWQFLELIQANGNTMPRAVPDEERQAQPFDPQRFQDAVVMPWYRNQDQPQYFYVAEICPHLSPLSCFPGDNYRTFKHYYLVKYGLTIQNTSQ--PLLDVDHTSARLNFLTPRYVNRKGVALPTSSEETKRAKRENLEQKQILVPELCTVHPFPASLWRTAVCLPCILYRINGLLLADD 1273
BLAST of Endoribonuclease Dicer vs. Ensembl Fly
Match: Dcr-2 (gene:FBgn0034246 transcript:FBtr0340217) HSP 1 Score: 134.42 bits (337), Expect = 3.994e-31 Identity = 165/689 (23.95%), Postives = 304/689 (44.12%), Query Frame = 2 Query: 38 MKTRDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDYLN------VKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISE--HVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIARE----FCGNIKETIINY-ETESFPDLVAIFKSAIEKIESFIPVD----GHHSNDNSGSLNAEQNNFKT--LKKYLSESFSVLSTLGLVPCK-----LITDMFIEDLHGA------VNNDSMFFDEKTKLYLDEFLSCLKFI----NSNIESYISKSDMLMCSEKCKKLINLILDLKSKNEDFKNTISILIFINEKIHVLAL-NVLLGILPKINKAFDFINSNFIIGHST------SQYPKKLQMSVTKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFT--HETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSE-RNVTPSEDDKYDYVPY-GENGPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYF----QDEFNF---------HHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLP 1930 +K R YQ+ + + N I+ L TGSGKTF+A +++K + K+ F+ L Q +++ K +G++ G+ VD+WT+ WS +I K +++V TA+VFLD++ LS + +I DE HH T + + + T +++GLT LIKG + ++ +K+LE +IT D E + E ++++ E + + + IEK ++ +D G S SL ++ K +K+ ++ + G+ LI + I+ ++ ++ EK + L + L + + N N E I M + K ++ + + L + ++D K+ I L+F+ + + +LL + + + + F++G + S +K Q S +Q ++V+EEG+DV CN V DP + F Y+QSKGRAR +++++FT E +KT QI ++ + ++ + LK RV+E K + E + D D P+ ENG + N+A ++L+RY + ++ + +F +DE + H +S+ +P+ + I P N + AK +ACK+L+ EL+ +P Sbjct: 6 IKPRGYQLRLVDHLTKSNGIVYLPTGSGKTFVAILVLKRFSQDFDKPIESGGKRALFMCNTVELARQQAMAVRRCTNFK--VGFYVGE---QGVDDWTRGMWSDEIKKNQVLVGTAQVFLDMVTQTYVALSSLSVVIIDECHHGTGHHPFREFMRLFTIANQTKLPRVVGLTGVLIKG-NEITNVATKLKELEITYRGNIITVSDTKEMENVMLYATKPTEVMVSFPHQEQVLTVTRLISAEIEKF--YVSLDLMNIGVQPIRRSKSLQCLRDPSKKSFVKQLFNDFLYQMKEYGIYAASIAIISLIVEFDIKRRQAETLSVKLMHRTALTLCEKIRHLLVQKLQDMTYDDDDDNVNTEEVI----MNFSTPKVQRFL-MSLKVSFADKDPKD-ICCLVFVERRYTCKCIYGLLLNYIQSTPELRNVLTPQFMVGRNNISPDFESVLERKWQKSAIQQFRDGNANLMI------CSSVLEEGIDVQACNHVFILDPVKTFNMYVQSKGRARTTEAKFVLFTADKEREKTIQQIYQYRKAHNDIAEYLKDRVLE----KTEPELYEIKGHFQDDID--PFTNENGAVLLPNNALAILHRYCQTIPTDAFG---FVIPWFHVLQEDERDRIFGVSAKGKHVISINMPVNCMLRDTIYSDPMDNVKTAKISAAFKACKVLYSLGELNERFVP 665 HSP 2 Score: 129.413 bits (324), Expect = 1.533e-29 Identity = 76/197 (38.58%), Postives = 117/197 (59.39%), Query Frame = 2 Query: 3296 NSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLV----PDNELNADIEISPKLAFQNISMDTDIPQSIND--LEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVV 3868 +D S EVN + I NK +AD EA++G I+ NYG+ ++ + L++ + P +L ++E+ K N++ IN LEK L Y FK++ Y++QA+THPS NR YQ LEF+GD+++DFL++ YI NN K+NPG +T+LRSALVNN T A + V+ H ++ ++K+ I+ K V Sbjct: 1344 GADFSSEVNFCVGLVTIPNKVIADTLEALLGVIVKNYGLQHAFKMLEYFKICRADIDKPLTQL-LNLELGGKKMRANVNTTEIDGFLINHYYLEKNLGYTFKDRRYLLQALTHPSYPTNRITGSYQELEFIGDAILDFLISAYIFENNTKMNPGALTDLRSALVNNTTLACICVRHRLHFFILAENAKLSEIISKFV 1539
BLAST of Endoribonuclease Dicer vs. Ensembl Fly
Match: Dcr-2 (gene:FBgn0034246 transcript:FBtr0086904) HSP 1 Score: 134.42 bits (337), Expect = 4.385e-31 Identity = 165/689 (23.95%), Postives = 304/689 (44.12%), Query Frame = 2 Query: 38 MKTRDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDYLN------VKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISE--HVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIARE----FCGNIKETIINY-ETESFPDLVAIFKSAIEKIESFIPVD----GHHSNDNSGSLNAEQNNFKT--LKKYLSESFSVLSTLGLVPCK-----LITDMFIEDLHGA------VNNDSMFFDEKTKLYLDEFLSCLKFI----NSNIESYISKSDMLMCSEKCKKLINLILDLKSKNEDFKNTISILIFINEKIHVLAL-NVLLGILPKINKAFDFINSNFIIGHST------SQYPKKLQMSVTKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFT--HETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSE-RNVTPSEDDKYDYVPY-GENGPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYF----QDEFNF---------HHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLP 1930 +K R YQ+ + + N I+ L TGSGKTF+A +++K + K+ F+ L Q +++ K +G++ G+ VD+WT+ WS +I K +++V TA+VFLD++ LS + +I DE HH T + + + T +++GLT LIKG + ++ +K+LE +IT D E + E ++++ E + + + IEK ++ +D G S SL ++ K +K+ ++ + G+ LI + I+ ++ ++ EK + L + L + + N N E I M + K ++ + + L + ++D K+ I L+F+ + + +LL + + + + F++G + S +K Q S +Q ++V+EEG+DV CN V DP + F Y+QSKGRAR +++++FT E +KT QI ++ + ++ + LK RV+E K + E + D D P+ ENG + N+A ++L+RY + ++ + +F +DE + H +S+ +P+ + I P N + AK +ACK+L+ EL+ +P Sbjct: 6 IKPRGYQLRLVDHLTKSNGIVYLPTGSGKTFVAILVLKRFSQDFDKPIESGGKRALFMCNTVELARQQAMAVRRCTNFK--VGFYVGE---QGVDDWTRGMWSDEIKKNQVLVGTAQVFLDMVTQTYVALSSLSVVIIDECHHGTGHHPFREFMRLFTIANQTKLPRVVGLTGVLIKG-NEITNVATKLKELEITYRGNIITVSDTKEMENVMLYATKPTEVMVSFPHQEQVLTVTRLISAEIEKF--YVSLDLMNIGVQPIRRSKSLQCLRDPSKKSFVKQLFNDFLYQMKEYGIYAASIAIISLIVEFDIKRRQAETLSVKLMHRTALTLCEKIRHLLVQKLQDMTYDDDDDNVNTEEVI----MNFSTPKVQRFL-MSLKVSFADKDPKD-ICCLVFVERRYTCKCIYGLLLNYIQSTPELRNVLTPQFMVGRNNISPDFESVLERKWQKSAIQQFRDGNANLMI------CSSVLEEGIDVQACNHVFILDPVKTFNMYVQSKGRARTTEAKFVLFTADKEREKTIQQIYQYRKAHNDIAEYLKDRVLE----KTEPELYEIKGHFQDDID--PFTNENGAVLLPNNALAILHRYCQTIPTDAFG---FVIPWFHVLQEDERDRIFGVSAKGKHVISINMPVNCMLRDTIYSDPMDNVKTAKISAAFKACKVLYSLGELNERFVP 665 HSP 2 Score: 129.413 bits (324), Expect = 1.521e-29 Identity = 76/197 (38.58%), Postives = 117/197 (59.39%), Query Frame = 2 Query: 3296 NSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLV----PDNELNADIEISPKLAFQNISMDTDIPQSIND--LEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVV 3868 +D S EVN + I NK +AD EA++G I+ NYG+ ++ + L++ + P +L ++E+ K N++ IN LEK L Y FK++ Y++QA+THPS NR YQ LEF+GD+++DFL++ YI NN K+NPG +T+LRSALVNN T A + V+ H ++ ++K+ I+ K V Sbjct: 1345 GADFSSEVNFCVGLVTIPNKVIADTLEALLGVIVKNYGLQHAFKMLEYFKICRADIDKPLTQL-LNLELGGKKMRANVNTTEIDGFLINHYYLEKNLGYTFKDRRYLLQALTHPSYPTNRITGSYQELEFIGDAILDFLISAYIFENNTKMNPGALTDLRSALVNNTTLACICVRHRLHFFILAENAKLSEIISKFV 1540
BLAST of Endoribonuclease Dicer vs. Ensembl Fly
Match: drosha (gene:FBgn0026722 transcript:FBtr0088850) HSP 1 Score: 69.3218 bits (168), Expect = 3.078e-11 Identity = 71/284 (25.00%), Postives = 128/284 (45.07%), Query Frame = 2 Query: 3038 KTCNDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSD--ESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGIDYSIQYLQ-------------WLNLPLVPDNELNADIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPS-SIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSK 3841 +T ++ T+ E+ E LGD+ ++F++S +LF F EG L R+ ++ N++L LA + L+++ Y + SD +E+ ++ +A+C EA++G++L + GI + + W NLP P E L ++ + + + E + FK+ + +A T S + QRLEFLGD+V+ + + Y+ + + + G ++ LRS+LVNN T A + G KY Y + K Sbjct: 824 ETVSNITHNERLEFLGDAVVEFLSSIHLF-FMFPELEEGGLATYRAAIVQNQHLALLAKKLQLEEFMLYAH---------------GSDLCHELELRHA----------MANCFEALMGALLLDGGIKVADEVFTDALFRQDEKLLSIWKNLPEHPLQEQEP-------LGDRSCIDSYRVLKELTKFEDSIGIKFKHIRLLARAFTDRSIGFTHLTLGSNQRLEFLGDTVLQLICSEYLYRHFPEHHEGHLSLLRSSLVNNRTQAVVCDDLGMPKYAVYANPK 1074
BLAST of Endoribonuclease Dicer vs. Ensembl Zebrafish
Match: dicer1 (dicer 1, ribonuclease type III [Source:NCBI gene;Acc:324724]) HSP 1 Score: 222.631 bits (566), Expect = 5.062e-58 Identity = 270/1067 (25.30%), Postives = 476/1067 (44.61%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDY---LNVKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDN-WTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIARE-FCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTL-KKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKS--DMLMCSEKCKKLINLILDLKS--------------KNED------------------------------FKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQ-YPKKLQMSV--TKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVF--THETQKTEAQIQKFNLINEEL-NKILKSRVIED-ELNKDDSERNVTPSEDDKYDYVPYGEN-GPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNRFDNNLVIQESNDIEIIKYE-----------KYTATKLSTKFQNLS-------------IPANKLYLYSIKYTPSVFL--KLGINDISMF---DVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFE-YNLPVNSINYSLLLKCHKILFNIYSERIRRFDKNIF-FDPSKAKSS---IILTILKD-NKIDFN-KIKEIITNPIVDNLIKNDQ-----------SSWVGSVIRPNYIT-NEFGAFVISKVCHDMNADSIFKND--MTYEKFLNKKYNIILSPNEKLSPLIKV------IRLTTIQSCVNKERRFLRNNRKLQRINESECY-----FVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDF 2884 R YQ+E+ A N I+ LNTGSGKTFIA +LIK+ + ++ K+ FLV +S V Q ++++ + L ++G + + SW + W ++ ++ VMT +FL +L + LS++ L+FDE H Y +I + S +ILGLTA ++ G D+E ++ LE L T+ D + + + +E +++ + D + + + +++ + + ND + S++ E + + K+ L++ +VL+ LG + + +L + ++ + K L+ D + L+ I++ E + S + D+ + K +L+ ++ + K +N+D F N + +IF+ + + LN L+ K + +I+SNFI GHS + P+ QM V KQ E+L+ F+ + N+LIAT+++EEG+D+ CN+VV+FD P ++RSY+QSKGRARA S YI+ + T+ + ++ + I + L NK KS D EL + NV P YV E+ GPR+T+N+A +NRY +L + + + + +L LP+ +P + G + R A++ V L C+ LHK ELD L+P + E +KYE STK + S +P YLY I + L +L ++ D + GIL PI + FP+Y G ++ I+ + +++ L+ + H+ +F+ I R +K F P +A S+ + L I++D N +D + K E I I N Q + +VI P Y ++ F ++ V D+ S F + T+ ++ KYN+ LS + PL+ V + L T + +N++ + L + +R + E VP L + +SLW + P+I +R++ +L+ E+ Sbjct: 35 RKYQVELLEAALEHNTIVCLNTGSGKTFIAVLLIKELSHQIRGENGKRTVFLVNAASSVAQQASTVRTHSDL--QVGDYMSEDMTSWPEEMWNREMIENQVL-----VMTCHIFLHVLKNGVLPLSKINLLVFDECHLAITGHPYREIMKICEGCPSCPRILGLTASILNGKCDPCDLEEKIQNLEKILQSNAETATDLVVLDRYASQPREEVLD--CGQYQDQSGLSERLLNELDEAL----NFLNDCNLSVHREDRDPTFISKQVLNDCRAVLTVLGPWCADKAAGIMVRELQKYIKHEQEELNRKFLLFTD---TILRKIHALCEEHFSPASLDLKFVTPKVIRLLEILHEYKPFERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEAEAKEKTEANFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHSIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPVSNYIMLADSERTKTFQEDLKTYKAIEKILRNKCSKSAECNDFELEPVTDDDNVLPP------YVLRSEDGGPRVTMNTAIGHVNRYCARLPSDPFTHLAPKCKTVEMNTGGYRSTLFLPINSPLRVPVTGPVMNCARLAEKAVALLCCEKLHKIGELDDHLMPVGK-------------ETVKYEEELDLHDEEETSVPGRPGSTKRRQCSPKAIPECLRGCYPVPEQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPR---IPHFPVYTRSGEVTISIELQKSGFSLSAEQLELITRLHQYIFS----HILRLEKPALEFKPVEADSAYCVLPLNIVEDSNTLDLDFKFMEDIEKSEARIGIPNTQYTKQNPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLSNVNQ--PLLDVDHTSSRLNLLTPRH-LNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEEL 1056 HSP 2 Score: 131.724 bits (330), Expect = 1.130e-41 Identity = 71/197 (36.04%), Postives = 120/197 (60.91%), Query Frame = 2 Query: 3344 IKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPDNELNAD---------IEISPKLAFQNISMDTDIPQSIN---DLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE-ELLNNQLQ 3895 I +K++ADC EA++G LT+ G + +L L L ++P + ++ ++I P+ F++ + + I+ + E +NY FKNK Y++QA TH S N CYQRLEFLGD+++D+L+T ++ + ++ +PG +T+LRSALVNN FA L+VK+ +HKY ++FH++ V+ +L N++Q Sbjct: 1530 IADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPPEKQSSGGSAELQYGWLKIPPRCMFEHPDAERTLNHLISGFLNFESKINYTFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQ 1726 HSP 3 Score: 60.4622 bits (145), Expect = 1.130e-41 Identity = 28/60 (46.67%), Postives = 39/60 (65.00%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQ 4112 V K +GDIFES+AGAI++DS L+ VW VY M ++KF N+PR+ + L E P+ Sbjct: 1747 VPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPE 1806 HSP 4 Score: 76.2554 bits (186), Expect = 3.072e-13 Identity = 44/107 (41.12%), Postives = 63/107 (58.88%), Query Frame = 2 Query: 3047 NDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLAD 3367 +D NLE+ E+LGDSFLK + YLF D +EG+L+ RS+ +SN NL+ L +KGL + F+P N+LPP +VV S + +S E NK+LA+ Sbjct: 1289 SDGFNLERLEMLGDSFLKHAITTYLFCTYPDA-HEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSSTDKWDSDE-----NKDLAN 1389
BLAST of Endoribonuclease Dicer vs. Ensembl Zebrafish
Match: dicer1 (dicer 1, ribonuclease type III [Source:NCBI gene;Acc:324724]) HSP 1 Score: 221.476 bits (563), Expect = 1.376e-57 Identity = 269/1067 (25.21%), Postives = 475/1067 (44.52%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDY---LNVKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDN-WTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIARE-FCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTL-KKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKS--DMLMCSEKCKKLINLILDLKS--------------KNED------------------------------FKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQ-YPKKLQMSV--TKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVF--THETQKTEAQIQKFNLINEEL-NKILKSRVIED-ELNKDDSERNVTPSEDDKYDYVPYGEN-GPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNRFDNNLVIQESNDIEIIKYE-----------KYTATKLSTKFQNL-------------SIPANKLYLYSIKYTPSVFL--KLGINDISMF---DVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFE-YNLPVNSINYSLLLKCHKILFNIYSERIRRFDKNIF-FDPSKAKSS---IILTILKD-NKIDFN-KIKEIITNPIVDNLIKNDQ-----------SSWVGSVIRPNYIT-NEFGAFVISKVCHDMNADSIFKND--MTYEKFLNKKYNIILSPNEKLSPLIKV------IRLTTIQSCVNKERRFLRNNRKLQRINESECY-----FVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDF 2884 R YQ+E+ A N I+ LNTGSGKTFIA +LIK+ + ++ K+ FLV +S V Q ++++ + L ++G + + SW + W ++ ++ VMT +FL +L + LS++ L+FDE H Y +I + S +ILGLTA ++ G D+E ++ LE L T+ D + + + +E +++ + D + + + +++ + + ND + S++ E + + K+ L++ +VL+ LG + + +L + ++ + K L+ D + L+ I++ E + S + D+ + K +L+ ++ + K +N+D F N + +IF+ + + LN L+ K + +I+SNFI GHS + P+ QM V KQ E+L+ F+ + N+LIAT+++EEG+D+ CN+VV+FD P ++RSY+QSKGRARA S YI+ + T+ + ++ + I + L NK KS D EL + NV P YV E+ GPR+T+N+A +NRY +L + + + + +L LP+ +P + G + R A++ V L C+ LHK ELD L+P + E +KYE STK + +P YLY I + L +L ++ D + GIL PI + FP+Y G ++ I+ + +++ L+ + H+ +F+ I R +K F P +A S+ + L I++D N +D + K E I I N Q + +VI P Y ++ F ++ V D+ S F + T+ ++ KYN+ LS + PL+ V + L T + +N++ + L + +R + E VP L + +SLW + P+I +R++ +L+ E+ Sbjct: 35 RKYQVELLEAALEHNTIVCLNTGSGKTFIAVLLIKELSHQIRGENGKRTVFLVNAASSVAQQASTVRTHSDL--QVGDYMSEDMTSWPEEMWNREMIENQVL-----VMTCHIFLHVLKNGVLPLSKINLLVFDECHLAITGHPYREIMKICEGCPSCPRILGLTASILNGKCDPCDLEEKIQNLEKILQSNAETATDLVVLDRYASQPREEVLD--CGQYQDQSGLSERLLNELDEAL----NFLNDCNLSVHREDRDPTFISKQVLNDCRAVLTVLGPWCADKAAGIMVRELQKYIKHEQEELNRKFLLFTD---TILRKIHALCEEHFSPASLDLKFVTPKVIRLLEILHEYKPFERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEAEAKEKTEANFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHSIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPVSNYIMLADSERTKTFQEDLKTYKAIEKILRNKCSKSAECNDFELEPVTDDDNVLPP------YVLRSEDGGPRVTMNTAIGHVNRYCARLPSDPFTHLAPKCKTVEMNTGGYRSTLFLPINSPLRVPVTGPVMNCARLAEKAVALLCCEKLHKIGELDDHLMPVGK-------------ETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRGCYPVPEQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPR---IPHFPVYTRSGEVTISIELQKSGFSLSAEQLELITRLHQYIFS----HILRLEKPALEFKPVEADSAYCVLPLNIVEDSNTLDLDFKFMEDIEKSEARIGIPNTQYTKQNPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLSNVNQ--PLLDVDHTSSRLNLLTPRH-LNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEEL 1056 HSP 2 Score: 131.724 bits (330), Expect = 1.084e-41 Identity = 71/197 (36.04%), Postives = 120/197 (60.91%), Query Frame = 2 Query: 3344 IKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPDNELNAD---------IEISPKLAFQNISMDTDIPQSIN---DLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE-ELLNNQLQ 3895 I +K++ADC EA++G LT+ G + +L L L ++P + ++ ++I P+ F++ + + I+ + E +NY FKNK Y++QA TH S N CYQRLEFLGD+++D+L+T ++ + ++ +PG +T+LRSALVNN FA L+VK+ +HKY ++FH++ V+ +L N++Q Sbjct: 1530 IADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPPEKQSSGGSAELQYGWLKIPPRCMFEHPDAERTLNHLISGFLNFESKINYTFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQ 1726 HSP 3 Score: 60.4622 bits (145), Expect = 1.084e-41 Identity = 28/60 (46.67%), Postives = 39/60 (65.00%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQ 4112 V K +GDIFES+AGAI++DS L+ VW VY M ++KF N+PR+ + L E P+ Sbjct: 1747 VPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPE 1806 HSP 4 Score: 76.2554 bits (186), Expect = 2.945e-13 Identity = 44/107 (41.12%), Postives = 63/107 (58.88%), Query Frame = 2 Query: 3047 NDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLAD 3367 +D NLE+ E+LGDSFLK + YLF D +EG+L+ RS+ +SN NL+ L +KGL + F+P N+LPP +VV S + +S E NK+LA+ Sbjct: 1289 SDGFNLERLEMLGDSFLKHAITTYLFCTYPDA-HEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSSTDKWDSDE-----NKDLAN 1389
BLAST of Endoribonuclease Dicer vs. Ensembl Zebrafish
Match: dicer1 (dicer 1, ribonuclease type III [Source:NCBI gene;Acc:324724]) HSP 1 Score: 211.075 bits (536), Expect = 2.259e-54 Identity = 233/897 (25.98%), Postives = 408/897 (45.48%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDY---LNVKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDN-WTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIARE-FCGNIKETIIN---YETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTL-KKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKS--DMLMCSEKCKKLINLILDLKS--------------KNED------------------------------FKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQ-YPKKLQMSV--TKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVF--THETQKTEAQIQKFNLINEEL-NKILKSRVIED-ELNKDDSERNVTPSEDDKYDYVPYGEN-GPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNRFDNNLVIQESNDIEIIKYE-----------KYTATKLSTKFQNLS-------------IPANKLYLYSIKYTPSVFL--KLGINDISMF---DVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFE-YNLPVNSINYSLLLKCHKILFNIYSERIRRFDKNIF-FDPSKAKSS---IILTILKDN---KIDFNKIKEI 2437 R YQ+E+ A N I+ LNTGSGKTFIA +LIK+ + ++ K+ FLV +S V Q ++++ + L ++G + + SW + W ++ ++ VMT +FL +L + LS++ L+FDE H Y +I + S +ILGLTA ++ G D+E ++ LE L T+ D + + + +E +++ Y+ +S L + +++ +F+ ND + S++ E + + K+ L++ +VL+ LG + + +L + ++ + K L+ D + L+ I++ E + S + D+ + K +L+ ++ + K +N+D F N + +IF+ + + LN L+ K + +I+SNFI GHS + P+ QM V KQ E+L+ F+ + N+LIAT+++EEG+D+ CN+VV+FD P ++RSY+QSKGRARA S YI+ + T+ + ++ + I + L NK KS D EL + NV P YV E+ GPR+T+N+A +NRY +L + + + + +L LP+ +P + G + R A++ V L C+ LHK ELD L+P + E +KYE STK + S +P YLY I + L +L ++ D + GIL PI + FP+Y G ++ I+ + +++ L+ + H+ +F+ I R +K F P +A S+ + L I++D+ +DF +++I Sbjct: 35 RKYQVELLEAALEHNTIVCLNTGSGKTFIAVLLIKELSHQIRGENGKRTVFLVNAASSVAQQASTVRTHSDL--QVGDYMSEDMTSWPEEMWNREMIENQVL-----VMTCHIFLHVLKNGVLPLSKINLLVFDECHLAITGHPYREIMKICEGCPSCPRILGLTASILNGKCDPCDLEEKIQNLEKILQSNAETATDLVVLDRYASQPREEVLDCGQYQDQS--GLSERLLNELDEALNFL-------NDCNLSVHREDRDPTFISKQVLNDCRAVLTVLGPWCADKAAGIMVRELQKYIKHEQEELNRKFLLFTD---TILRKIHALCEEHFSPASLDLKFVTPKVIRLLEILHEYKPFERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEAEAKEKTEANFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHSIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPVSNYIMLADSERTKTFQEDLKTYKAIEKILRNKCSKSAECNDFELEPVTDDDNVLPP------YVLRSEDGGPRVTMNTAIGHVNRYCARLPSDPFTHLAPKCKTVEMNTGGYRSTLFLPINSPLRVPVTGPVMNCARLAEKAVALLCCEKLHKIGELDDHLMPVGK-------------ETVKYEEELDLHDEEETSVPGRPGSTKRRQCSPKAIPECLRGCYPVPEQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPR---IPHFPVYTRSGEVTISIELQKSGFSLSAEQLELITRLHQYIFS----HILRLEKPALEFKPVEADSAYCVLPLNIVEDSNTLDLDFKFMEDI 886 HSP 2 Score: 131.724 bits (330), Expect = 1.139e-41 Identity = 71/197 (36.04%), Postives = 120/197 (60.91%), Query Frame = 2 Query: 3344 IKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPDNELNAD---------IEISPKLAFQNISMDTDIPQSIN---DLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE-ELLNNQLQ 3895 I +K++ADC EA++G LT+ G + +L L L ++P + ++ ++I P+ F++ + + I+ + E +NY FKNK Y++QA TH S N CYQRLEFLGD+++D+L+T ++ + ++ +PG +T+LRSALVNN FA L+VK+ +HKY ++FH++ V+ +L N++Q Sbjct: 1570 IADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPPEKQSSGGSAELQYGWLKIPPRCMFEHPDAERTLNHLISGFLNFESKINYTFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQ 1766 HSP 3 Score: 60.4622 bits (145), Expect = 1.139e-41 Identity = 28/60 (46.67%), Postives = 39/60 (65.00%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQ 4112 V K +GDIFES+AGAI++DS L+ VW VY M ++KF N+PR+ + L E P+ Sbjct: 1787 VPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPE 1846 HSP 4 Score: 76.2554 bits (186), Expect = 2.933e-13 Identity = 44/107 (41.12%), Postives = 63/107 (58.88%), Query Frame = 2 Query: 3047 NDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLAD 3367 +D NLE+ E+LGDSFLK + YLF D +EG+L+ RS+ +SN NL+ L +KGL + F+P N+LPP +VV S + +S E NK+LA+ Sbjct: 1329 SDGFNLERLEMLGDSFLKHAITTYLFCTYPDA-HEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSSTDKWDSDE-----NKDLAN 1429
BLAST of Endoribonuclease Dicer vs. Ensembl Zebrafish
Match: drosha (drosha ribonuclease III [Source:ZFIN;Acc:ZDB-GENE-070209-23]) HSP 1 Score: 82.8037 bits (203), Expect = 2.486e-15 Identity = 66/265 (24.91%), Postives = 121/265 (45.66%), Query Frame = 2 Query: 3065 EQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGIDYSIQYLQ------------WLNLPLVPDNELNADIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANR-KFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKY 3820 E+ E LGD+ ++F+TS +L+H + EG L R+ ++ N++L LA + LD++ Y + P+ RE L +A+C EA+IG++ G++ + WLN P P +++ L + + + + Q + + E + F + + +A T + N QR+EFLGDS++ + T Y+ ++ + G +T LRS+LVNN T A ++ + G ++ Sbjct: 886 ERLEFLGDAVVEFLTSVHLYHLFPSLE-EGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHG--------PDLC-------------RESDL--RHAMANCFEALIGAVYLEGGLEEAKDLFGRLLFNTEDMREVWLNYPPHP-------LQVQEPLTDRQLIESSPVLQKLTNFEDAIGVLFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEF 1119
BLAST of Endoribonuclease Dicer vs. Ensembl Zebrafish
Match: drosha (drosha ribonuclease III [Source:ZFIN;Acc:ZDB-GENE-070209-23]) HSP 1 Score: 82.8037 bits (203), Expect = 2.486e-15 Identity = 66/265 (24.91%), Postives = 121/265 (45.66%), Query Frame = 2 Query: 3065 EQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGIDYSIQYLQ------------WLNLPLVPDNELNADIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANR-KFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKY 3820 E+ E LGD+ ++F+TS +L+H + EG L R+ ++ N++L LA + LD++ Y + P+ RE L +A+C EA+IG++ G++ + WLN P P +++ L + + + + Q + + E + F + + +A T + N QR+EFLGDS++ + T Y+ ++ + G +T LRS+LVNN T A ++ + G ++ Sbjct: 886 ERLEFLGDAVVEFLTSVHLYHLFPSLE-EGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHG--------PDLC-------------RESDL--RHAMANCFEALIGAVYLEGGLEEAKDLFGRLLFNTEDMREVWLNYPPHP-------LQVQEPLTDRQLIESSPVLQKLTNFEDAIGVLFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEF 1119
BLAST of Endoribonuclease Dicer vs. Ensembl Xenopus
Match: cox6b1 (cytochrome c oxidase subunit VIb polypeptide 1 (ubiquitous) [Source:Xenbase;Acc:XB-GENE-5945264]) HSP 1 Score: 220.705 bits (561), Expect = 2.627e-57 Identity = 267/1063 (25.12%), Postives = 473/1063 (44.50%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDY-LNVKSD-----KKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIAREFCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKS--DMLMCSEKCKKLINLILDLKS--------------KNED-------------------------------FKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQ-YPKKLQMSV--TKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVF--THETQKTEAQIQKFNLINEELNKILKSRVIED-ELNKDDSERNVTPSEDDKYDYVPY----GENGPRITLNSASSVLNRYLGKLRCNSISE--PKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNR--------------FDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFL--KLGINDISMF---DVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKF-EYNLPVNSINYSLLLKCHKILFNIYSERIRRFDKNIF-FDPSKAKSS---IILTILKDNK---IDFNKIKEIITNPIVDNLIKNDQSS-----------WVGSVIRPNYIT-NEFGAFVISKVCHDMNADSIFKND--MTYEKFLNKKYNIILSPNEKLSPLIKV------IRLTTIQSCVNKERRFLRNNRKLQRINESECY-----FVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDF 2884 R YQ+E+ A + N I+ LN+GSGKTFIA +L K+ ++ D K+ FLV + V Q ++++ + LK +G + Q + W K++W + ++++VMT +FL++L +LS + L+FDE H + Y +I + +ILGLTA ++ G +D+E +++LE L T+ D + + + I+ + + D +++ + +++ + + N + + E+++ K+ LS+ +VL LG + M + +L + ++ K L+ D + L+ I++ E + S + DM + K KL+ ++ K +N+D F N + +IF+ + + LN L+ K + +I+SNFI GH + P+ QM V KQ E+L+ F+ + N+LIAT+++EEG+D+ CN+VV+FD P ++RSY+QSKGRARA S YI+ + + + E ++ + I KIL+++ + + +SE P DD + PY + PR+T+N+A +NRY +L + + PK F D + +L LP+ +P I G P + R A + V L CK LH+ ELD L+P + + ++ + + Y K L + P YLY I + L +L ++ D + GIL PI Q FP+Y G ++ I+ + +N L+ + H+ +F+ I R +K F P+ A + + L ++ D+ IDF K E I I N Q S + +VI P Y ++ F ++ V D+ S F + T+ ++ KYN+ L+ + PL+ V + L T + +N++ + L + +R + E VP L + +SLW + P+I +R++ +L+ E+ Sbjct: 35 RKYQVELLEAALDHNTIVCLNSGSGKTFIAVLLSKELSYQIRGDFSKNTKRTVFLVNSEKQVSQQVSAVRTHTDLK--VGEYSDQEKTQC---WAKERWYLEFETHQVLVMTCHIFLNVLKSGNVSLSNINLLVFDECHLAIQDHPYREIMKICESCQPCPRILGLTASILNGKCDPRDLEEKIQKLEEILRSNAETATDLVVLDRYASQPCEIV-LDCGPYIDKSGLYQRLLNELDEAL---NFLIDCNISTHSKERDSTLISKQILSDCQTVLLVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTD---TILRKIHALCEEHFSPASLDMKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDEDEEIEEKEKTETSFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPSEYRSYVQSKGRARAPISNYIMLADSDKIKAFEEDLKTYKAI----EKILRNKCSKSIDCGNTESE----PIVDDDEIFPPYVLRQDDGSPRVTINTAIGHINRYCARLPSDPFTHLAPKCKTREFPD--GLYRSTLYLPINSPLRAPIVGPPMNCGRLADRAVALICCKKLHEIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRNSYPK---PGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQ---IPHFPVYTRSGEVTISIELKKSGFTLNLEQLELITRLHQYIFS----HILRLEKPALEFKPTVADCAYCVLPLNVVNDSGTLDIDF-KFVEDIEKSEARTGIPNTQYSAESPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQ--PLLDVDHTSSRLNLLTPRH-LNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPVPASLWRKAVCLPSILYRLHCLLTAEEL 1061 HSP 2 Score: 134.42 bits (337), Expect = 1.715e-42 Identity = 74/200 (37.00%), Postives = 122/200 (61.00%), Query Frame = 2 Query: 3344 IKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPD-NELNADI-----------EISPKLAFQNISMDTDIPQSIN---DLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE-ELLNNQLQ 3895 I +K++ADC EA++G LT+ G + +L L L ++P+ + N D+ +I P+ F++ + + I+ + EK +NY FKNK Y++QA TH S N CYQRLEFLGD+++D+L+T ++ + ++ +PG +T+LRSALVNN FA L+VK+ +HKY ++FH++ V+ +L N++Q Sbjct: 1503 IADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPEVRKTNTDLSSCKGIDYGYLKIPPRCMFEHPDAEKTLDHLISGFENFEKKINYPFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAISPELFHVIDDFVQFQLEKNEMQ 1702 HSP 3 Score: 61.2326 bits (147), Expect = 1.715e-42 Identity = 28/60 (46.67%), Postives = 40/60 (66.67%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQ 4112 V K +GDIFES+AGAI++DS L+ VW VY M+ ++KF N+PR+ + L E P+ Sbjct: 1723 VPKAMGDIFESLAGAIYMDSGMSLETVWHVYYPMMQPLIEKFSANVPRSPVRELLEMEPE 1782 HSP 4 Score: 71.633 bits (174), Expect = 1.058e-11 Identity = 39/90 (43.33%), Postives = 56/90 (62.22%), Query Frame = 2 Query: 3047 NDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVV---KNSDE 3307 +D NLE+ E+LGDSFLK + YLF D +EG+L+ RS+ +SN NL+ L +KG + F+P N+LPP ++V KNSD+ Sbjct: 1262 SDGFNLERLEMLGDSFLKHAITTYLFCTYPDA-HEGRLSYMRSKKVSNCNLYRLGKKKGSPSRMVVSIFDPPVNWLPPGYIVNQDKNSDK 1350
BLAST of Endoribonuclease Dicer vs. Ensembl Xenopus
Match: cox6b1 (cytochrome c oxidase subunit VIb polypeptide 1 (ubiquitous) [Source:Xenbase;Acc:XB-GENE-5945264]) HSP 1 Score: 220.705 bits (561), Expect = 3.064e-57 Identity = 267/1063 (25.12%), Postives = 473/1063 (44.50%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDY-LNVKSD-----KKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIAREFCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKS--DMLMCSEKCKKLINLILDLKS--------------KNED-------------------------------FKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQ-YPKKLQMSV--TKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVF--THETQKTEAQIQKFNLINEELNKILKSRVIED-ELNKDDSERNVTPSEDDKYDYVPY----GENGPRITLNSASSVLNRYLGKLRCNSISE--PKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNR--------------FDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFL--KLGINDISMF---DVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKF-EYNLPVNSINYSLLLKCHKILFNIYSERIRRFDKNIF-FDPSKAKSS---IILTILKDNK---IDFNKIKEIITNPIVDNLIKNDQSS-----------WVGSVIRPNYIT-NEFGAFVISKVCHDMNADSIFKND--MTYEKFLNKKYNIILSPNEKLSPLIKV------IRLTTIQSCVNKERRFLRNNRKLQRINESECY-----FVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDF 2884 R YQ+E+ A + N I+ LN+GSGKTFIA +L K+ ++ D K+ FLV + V Q ++++ + LK +G + Q + W K++W + ++++VMT +FL++L +LS + L+FDE H + Y +I + +ILGLTA ++ G +D+E +++LE L T+ D + + + I+ + + D +++ + +++ + + N + + E+++ K+ LS+ +VL LG + M + +L + ++ K L+ D + L+ I++ E + S + DM + K KL+ ++ K +N+D F N + +IF+ + + LN L+ K + +I+SNFI GH + P+ QM V KQ E+L+ F+ + N+LIAT+++EEG+D+ CN+VV+FD P ++RSY+QSKGRARA S YI+ + + + E ++ + I KIL+++ + + +SE P DD + PY + PR+T+N+A +NRY +L + + PK F D + +L LP+ +P I G P + R A + V L CK LH+ ELD L+P + + ++ + + Y K L + P YLY I + L +L ++ D + GIL PI Q FP+Y G ++ I+ + +N L+ + H+ +F+ I R +K F P+ A + + L ++ D+ IDF K E I I N Q S + +VI P Y ++ F ++ V D+ S F + T+ ++ KYN+ L+ + PL+ V + L T + +N++ + L + +R + E VP L + +SLW + P+I +R++ +L+ E+ Sbjct: 35 RKYQVELLEAALDHNTIVCLNSGSGKTFIAVLLSKELSYQIRGDFSKNTKRTVFLVNSEKQVSQQVSAVRTHTDLK--VGEYSDQEKTQC---WAKERWYLEFETHQVLVMTCHIFLNVLKSGNVSLSNINLLVFDECHLAIQDHPYREIMKICESCQPCPRILGLTASILNGKCDPRDLEEKIQKLEEILRSNAETATDLVVLDRYASQPCEIV-LDCGPYIDKSGLYQRLLNELDEAL---NFLIDCNISTHSKERDSTLISKQILSDCQTVLLVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTD---TILRKIHALCEEHFSPASLDMKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDEDEEIEEKEKTETSFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPSEYRSYVQSKGRARAPISNYIMLADSDKIKAFEEDLKTYKAI----EKILRNKCSKSIDCGNTESE----PIVDDDEIFPPYVLRQDDGSPRVTINTAIGHINRYCARLPSDPFTHLAPKCKTREFPD--GLYRSTLYLPINSPLRAPIVGPPMNCGRLADRAVALICCKKLHEIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRNSYPK---PGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQ---IPHFPVYTRSGEVTISIELKKSGFTLNLEQLELITRLHQYIFS----HILRLEKPALEFKPTVADCAYCVLPLNVVNDSGTLDIDF-KFVEDIEKSEARTGIPNTQYSAESPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQ--PLLDVDHTSSRLNLLTPRH-LNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPVPASLWRKAVCLPSILYRLHCLLTAEEL 1061 HSP 2 Score: 123.635 bits (309), Expect = 3.043e-39 Identity = 74/227 (32.60%), Postives = 121/227 (53.30%), Query Frame = 2 Query: 3344 IKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPD----------------------------NELNADI-----------EISPKLAFQNISMDTDIPQSIN---DLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE-ELLNNQLQ 3895 I +K++ADC EA++G LT+ G + +L L L ++P+ N D+ +I P+ F++ + + I+ + EK +NY FKNK Y++QA TH S N CYQRLEFLGD+++D+L+T ++ + ++ +PG +T+LRSALVNN FA L+VK+ +HKY ++FH++ V+ +L N++Q Sbjct: 1519 IADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPEVRKLVTNTNVISASSSYQNSTRDNCTLTARTNTDLSSCKGIDYGYLKIPPRCMFEHPDAEKTLDHLISGFENFEKKINYPFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAISPELFHVIDDFVQFQLEKNEMQ 1745 HSP 3 Score: 61.2326 bits (147), Expect = 3.043e-39 Identity = 28/60 (46.67%), Postives = 40/60 (66.67%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQ 4112 V K +GDIFES+AGAI++DS L+ VW VY M+ ++KF N+PR+ + L E P+ Sbjct: 1766 VPKAMGDIFESLAGAIYMDSGMSLETVWHVYYPMMQPLIEKFSANVPRSPVRELLEMEPE 1825 HSP 4 Score: 71.633 bits (174), Expect = 1.128e-11 Identity = 39/90 (43.33%), Postives = 56/90 (62.22%), Query Frame = 2 Query: 3047 NDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVV---KNSDE 3307 +D NLE+ E+LGDSFLK + YLF D +EG+L+ RS+ +SN NL+ L +KG + F+P N+LPP ++V KNSD+ Sbjct: 1281 SDGFNLERLEMLGDSFLKHAITTYLFCTYPDA-HEGRLSYMRSKKVSNCNLYRLGKKKGSPSRMVVSIFDPPVNWLPPGYIVNQDKNSDK 1369
BLAST of Endoribonuclease Dicer vs. Ensembl Xenopus
Match: cox6b1 (cytochrome c oxidase subunit VIb polypeptide 1 (ubiquitous) [Source:Xenbase;Acc:XB-GENE-5945264]) HSP 1 Score: 220.705 bits (561), Expect = 3.226e-57 Identity = 267/1063 (25.12%), Postives = 473/1063 (44.50%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDY-LNVKSD-----KKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIAREFCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKS--DMLMCSEKCKKLINLILDLKS--------------KNED-------------------------------FKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQ-YPKKLQMSV--TKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVF--THETQKTEAQIQKFNLINEELNKILKSRVIED-ELNKDDSERNVTPSEDDKYDYVPY----GENGPRITLNSASSVLNRYLGKLRCNSISE--PKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNR--------------FDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFL--KLGINDISMF---DVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKF-EYNLPVNSINYSLLLKCHKILFNIYSERIRRFDKNIF-FDPSKAKSS---IILTILKDNK---IDFNKIKEIITNPIVDNLIKNDQSS-----------WVGSVIRPNYIT-NEFGAFVISKVCHDMNADSIFKND--MTYEKFLNKKYNIILSPNEKLSPLIKV------IRLTTIQSCVNKERRFLRNNRKLQRINESECY-----FVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDF 2884 R YQ+E+ A + N I+ LN+GSGKTFIA +L K+ ++ D K+ FLV + V Q ++++ + LK +G + Q + W K++W + ++++VMT +FL++L +LS + L+FDE H + Y +I + +ILGLTA ++ G +D+E +++LE L T+ D + + + I+ + + D +++ + +++ + + N + + E+++ K+ LS+ +VL LG + M + +L + ++ K L+ D + L+ I++ E + S + DM + K KL+ ++ K +N+D F N + +IF+ + + LN L+ K + +I+SNFI GH + P+ QM V KQ E+L+ F+ + N+LIAT+++EEG+D+ CN+VV+FD P ++RSY+QSKGRARA S YI+ + + + E ++ + I KIL+++ + + +SE P DD + PY + PR+T+N+A +NRY +L + + PK F D + +L LP+ +P I G P + R A + V L CK LH+ ELD L+P + + ++ + + Y K L + P YLY I + L +L ++ D + GIL PI Q FP+Y G ++ I+ + +N L+ + H+ +F+ I R +K F P+ A + + L ++ D+ IDF K E I I N Q S + +VI P Y ++ F ++ V D+ S F + T+ ++ KYN+ L+ + PL+ V + L T + +N++ + L + +R + E VP L + +SLW + P+I +R++ +L+ E+ Sbjct: 47 RKYQVELLEAALDHNTIVCLNSGSGKTFIAVLLSKELSYQIRGDFSKNTKRTVFLVNSEKQVSQQVSAVRTHTDLK--VGEYSDQEKTQC---WAKERWYLEFETHQVLVMTCHIFLNVLKSGNVSLSNINLLVFDECHLAIQDHPYREIMKICESCQPCPRILGLTASILNGKCDPRDLEEKIQKLEEILRSNAETATDLVVLDRYASQPCEIV-LDCGPYIDKSGLYQRLLNELDEAL---NFLIDCNISTHSKERDSTLISKQILSDCQTVLLVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTD---TILRKIHALCEEHFSPASLDMKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDEDEEIEEKEKTETSFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPSEYRSYVQSKGRARAPISNYIMLADSDKIKAFEEDLKTYKAI----EKILRNKCSKSIDCGNTESE----PIVDDDEIFPPYVLRQDDGSPRVTINTAIGHINRYCARLPSDPFTHLAPKCKTREFPD--GLYRSTLYLPINSPLRAPIVGPPMNCGRLADRAVALICCKKLHEIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRNSYPK---PGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQ---IPHFPVYTRSGEVTISIELKKSGFTLNLEQLELITRLHQYIFS----HILRLEKPALEFKPTVADCAYCVLPLNVVNDSGTLDIDF-KFVEDIEKSEARTGIPNTQYSAESPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQ--PLLDVDHTSSRLNLLTPRH-LNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPVPASLWRKAVCLPSILYRLHCLLTAEEL 1073 HSP 2 Score: 123.25 bits (308), Expect = 3.305e-39 Identity = 74/227 (32.60%), Postives = 121/227 (53.30%), Query Frame = 2 Query: 3344 IKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPD----------------------------NELNADI-----------EISPKLAFQNISMDTDIPQSIN---DLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE-ELLNNQLQ 3895 I +K++ADC EA++G LT+ G + +L L L ++P+ N D+ +I P+ F++ + + I+ + EK +NY FKNK Y++QA TH S N CYQRLEFLGD+++D+L+T ++ + ++ +PG +T+LRSALVNN FA L+VK+ +HKY ++FH++ V+ +L N++Q Sbjct: 1534 IADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPEVRKLVTNTNVISASSSYQNSTRDNCTLTARTNTDLSSCKGIDYGYLKIPPRCMFEHPDAEKTLDHLISGFENFEKKINYPFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAISPELFHVIDDFVQFQLEKNEMQ 1760 HSP 3 Score: 61.2326 bits (147), Expect = 3.305e-39 Identity = 28/60 (46.67%), Postives = 40/60 (66.67%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQ 4112 V K +GDIFES+AGAI++DS L+ VW VY M+ ++KF N+PR+ + L E P+ Sbjct: 1781 VPKAMGDIFESLAGAIYMDSGMSLETVWHVYYPMMQPLIEKFSANVPRSPVRELLEMEPE 1840 HSP 4 Score: 71.633 bits (174), Expect = 1.092e-11 Identity = 39/90 (43.33%), Postives = 56/90 (62.22%), Query Frame = 2 Query: 3047 NDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVV---KNSDE 3307 +D NLE+ E+LGDSFLK + YLF D +EG+L+ RS+ +SN NL+ L +KG + F+P N+LPP ++V KNSD+ Sbjct: 1293 SDGFNLERLEMLGDSFLKHAITTYLFCTYPDA-HEGRLSYMRSKKVSNCNLYRLGKKKGSPSRMVVSIFDPPVNWLPPGYIVNQDKNSDK 1381
BLAST of Endoribonuclease Dicer vs. Ensembl Xenopus
Match: cox6b1 (cytochrome c oxidase subunit VIb polypeptide 1 (ubiquitous) [Source:Xenbase;Acc:XB-GENE-5945264]) HSP 1 Score: 218.394 bits (555), Expect = 1.670e-56 Identity = 266/1066 (24.95%), Postives = 473/1066 (44.37%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDY-LNVKSD-----KKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIAREFCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKS--DMLMCSEKCKKLINLILDLKS--------------KNED-------------------------------FKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQ-YPKKLQMSV--TKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVF--THETQKTEAQIQKFNLINEELNKILKSRVIED-ELNKDDSERNVTPSEDDKYDYVPY----GENGPRITLNSASSVLNRYLGKLRCNSISE--PKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNR--------------FDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFL--KLGINDISMF---DVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKF-EYNLPVNSINYSLLLKCHKILFNIYSERIRRFDKNIF-FDPSKAKSS---IILTILKDNK---IDFNKIKEIITNPIVDNLIKNDQSS-----------WVGSVIRPNYIT----NEFGAFVISKVCHDMNADSIFKND--MTYEKFLNKKYNIILSPNEKLSPLIKV------IRLTTIQSCVNKERRFLRNNRKLQRINESECY-----FVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDF 2884 R YQ+E+ A + N I+ LN+GSGKTFIA +L K+ ++ D K+ FLV + V Q ++++ + LK +G + Q + W K++W + ++++VMT +FL++L +LS + L+FDE H + Y +I + +ILGLTA ++ G +D+E +++LE L T+ D + + + I+ + + D +++ + +++ + + N + + E+++ K+ LS+ +VL LG + M + +L + ++ K L+ D + L+ I++ E + S + DM + K KL+ ++ K +N+D F N + +IF+ + + LN L+ K + +I+SNFI GH + P+ QM V KQ E+L+ F+ + N+LIAT+++EEG+D+ CN+VV+FD P ++RSY+QSKGRARA S YI+ + + + E ++ + I KIL+++ + + +SE P DD + PY + PR+T+N+A +NRY +L + + PK F D + +L LP+ +P I G P + R A + V L CK LH+ ELD L+P + + ++ + + Y K L + P YLY I + L +L ++ D + GIL PI Q FP+Y G ++ I+ + +N L+ + H+ +F+ I R +K F P+ A + + L ++ D+ IDF K E I I N Q S + +VI P + ++ F ++ V D+ S F + T+ ++ KYN+ L+ + PL+ V + L T + +N++ + L + +R + E VP L + +SLW + P+I +R++ +L+ E+ Sbjct: 35 RKYQVELLEAALDHNTIVCLNSGSGKTFIAVLLSKELSYQIRGDFSKNTKRTVFLVNSEKQVSQQVSAVRTHTDLK--VGEYSDQEKTQC---WAKERWYLEFETHQVLVMTCHIFLNVLKSGNVSLSNINLLVFDECHLAIQDHPYREIMKICESCQPCPRILGLTASILNGKCDPRDLEEKIQKLEEILRSNAETATDLVVLDRYASQPCEIV-LDCGPYIDKSGLYQRLLNELDEAL---NFLIDCNISTHSKERDSTLISKQILSDCQTVLLVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTD---TILRKIHALCEEHFSPASLDMKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDEDEEIEEKEKTETSFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPSEYRSYVQSKGRARAPISNYIMLADSDKIKAFEEDLKTYKAI----EKILRNKCSKSIDCGNTESE----PIVDDDEIFPPYVLRQDDGSPRVTINTAIGHINRYCARLPSDPFTHLAPKCKTREFPD--GLYRSTLYLPINSPLRAPIVGPPMNCGRLADRAVALICCKKLHEIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRNSYPK---PGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQ---IPHFPVYTRSGEVTISIELKKSGFTLNLEQLELITRLHQYIFS----HILRLEKPALEFKPTVADCAYCVLPLNVVNDSGTLDIDF-KFVEDIEKSEARTGIPNTQYSAESPFIFKLEDYQDAVIIPRQVIYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQ--PLLDVDHTSSRLNLLTPRH-LNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPVPASLWRKAVCLPSILYRLHCLLTAEEL 1064 HSP 2 Score: 123.25 bits (308), Expect = 3.172e-39 Identity = 74/227 (32.60%), Postives = 121/227 (53.30%), Query Frame = 2 Query: 3344 IKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPD----------------------------NELNADI-----------EISPKLAFQNISMDTDIPQSIN---DLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE-ELLNNQLQ 3895 I +K++ADC EA++G LT+ G + +L L L ++P+ N D+ +I P+ F++ + + I+ + EK +NY FKNK Y++QA TH S N CYQRLEFLGD+++D+L+T ++ + ++ +PG +T+LRSALVNN FA L+VK+ +HKY ++FH++ V+ +L N++Q Sbjct: 1528 IADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPEVRKLVTNTNVISASSSYQNSTRDNCTLTARTNTDLSSCKGIDYGYLKIPPRCMFEHPDAEKTLDHLISGFENFEKKINYPFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAISPELFHVIDDFVQFQLEKNEMQ 1754 HSP 3 Score: 61.2326 bits (147), Expect = 3.172e-39 Identity = 28/60 (46.67%), Postives = 40/60 (66.67%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQ 4112 V K +GDIFES+AGAI++DS L+ VW VY M+ ++KF N+PR+ + L E P+ Sbjct: 1775 VPKAMGDIFESLAGAIYMDSGMSLETVWHVYYPMMQPLIEKFSANVPRSPVRELLEMEPE 1834 HSP 4 Score: 74.7146 bits (182), Expect = 1.479e-12 Identity = 42/106 (39.62%), Postives = 62/106 (58.49%), Query Frame = 2 Query: 3047 NDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVV---KNSDESVEVNNSREFILIKNK 3355 +D NLE+ E+LGDSFLK + YLF D +EG+L+ RS+ +SN NL+ L +KG + F+P N+LPP ++V KNSD+ S E +++ K Sbjct: 1284 SDGFNLERLEMLGDSFLKHAITTYLFCTYPDA-HEGRLSYMRSKKVSNCNLYRLGKKKGSPSRMVVSIFDPPVNWLPPGYIVNQDKNSDKWESNETSGEDVMVNGK 1388
BLAST of Endoribonuclease Dicer vs. Ensembl Xenopus
Match: cox6b1 (cytochrome c oxidase subunit VIb polypeptide 1 (ubiquitous) [Source:Xenbase;Acc:XB-GENE-5945264]) HSP 1 Score: 210.69 bits (535), Expect = 3.841e-54 Identity = 269/1087 (24.75%), Postives = 473/1087 (43.51%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDY-LNVKSD-----KKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIAREFCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKS--DMLMCSEKCKKLINLILDLKS--------------KNED-------------------------------FKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQ-YPKKLQMSV--TKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVF--THETQKTEAQIQKFNLINEELNKILKSRVIED-ELNKDDSERNVTPSEDDKYDYVPY----GENGPRITLNSASSVLNRYLGKLRCNSISE--PKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNR--------------FDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFL--KLGINDISMF---DVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKF-EYNLPVNSINYSLLLKCHKILFNIYSERIRRFDKNIF-FDPSKAKSS---IILTILKDNK---IDFNKIKEIITNPIVDNLIKNDQSS-----------WVGSVIRPNY--------------ITNEFGA-----------FVISKVCHDMNADSIFKND--MTYEKFLNKKYNIILSPNEKLSPLIKV------IRLTTIQSCVNKERRFLRNNRKLQRINESECY-----FVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDF 2884 R YQ+E+ A + N I+ LN+GSGKTFIA +L K+ ++ D K+ FLV + V Q ++++ + LK +G + Q + W K++W + ++++VMT +FL++L +LS + L+FDE H + Y +I + +ILGLTA ++ G +D+E +++LE L T+ D + + + I+ + + D +++ + +++ + + N + + E+++ K+ LS+ +VL LG + M + +L + ++ K L+ D + L+ I++ E + S + DM + K KL+ ++ K +N+D F N + +IF+ + + LN L+ K + +I+SNFI GH + P+ QM V KQ E+L+ F+ + N+LIAT+++EEG+D+ CN+VV+FD P ++RSY+QSKGRARA S YI+ + + + E ++ + I KIL+++ + + +SE P DD + PY + PR+T+N+A +NRY +L + + PK F D + +L LP+ +P I G P + R A + V L CK LH+ ELD L+P + + ++ + + Y K L + P YLY I + L +L ++ D + GIL PI Q FP+Y G ++ I+ + +N L+ + H+ +F+ I R +K F P+ A + + L ++ D+ IDF K E I I N Q S + +VI P I F A F ++ V D+ S F + T+ ++ KYN+ L+ + PL+ V + L T + +N++ + L + +R + E VP L + +SLW + P+I +R++ +L+ E+ Sbjct: 35 RKYQVELLEAALDHNTIVCLNSGSGKTFIAVLLSKELSYQIRGDFSKNTKRTVFLVNSEKQVSQQVSAVRTHTDLK--VGEYSDQEKTQC---WAKERWYLEFETHQVLVMTCHIFLNVLKSGNVSLSNINLLVFDECHLAIQDHPYREIMKICESCQPCPRILGLTASILNGKCDPRDLEEKIQKLEEILRSNAETATDLVVLDRYASQPCEIV-LDCGPYIDKSGLYQRLLNELDEAL---NFLIDCNISTHSKERDSTLISKQILSDCQTVLLVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTD---TILRKIHALCEEHFSPASLDMKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDEDEEIEEKEKTETSFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPSEYRSYVQSKGRARAPISNYIMLADSDKIKAFEEDLKTYKAI----EKILRNKCSKSIDCGNTESE----PIVDDDEIFPPYVLRQDDGSPRVTINTAIGHINRYCARLPSDPFTHLAPKCKTREFPD--GLYRSTLYLPINSPLRAPIVGPPMNCGRLADRAVALICCKKLHEIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRNSYPK---PGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQ---IPHFPVYTRSGEVTISIELKKSGFTLNLEQLELITRLHQYIFS----HILRLEKPALEFKPTVADCAYCVLPLNVVNDSGTLDIDF-KFVEDIEKSEARTGIPNTQYSAESPFIFKLEDYQDAVIIPRQVMFFPFLQCYLFLKILQHFEADKYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQ--PLLDVDHTSSRLNLLTPRH-LNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPVPASLWRKAVCLPSILYRLHCLLTAEEL 1085 HSP 2 Score: 134.035 bits (336), Expect = 1.925e-42 Identity = 74/200 (37.00%), Postives = 122/200 (61.00%), Query Frame = 2 Query: 3344 IKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPD-NELNADI-----------EISPKLAFQNISMDTDIPQSIN---DLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE-ELLNNQLQ 3895 I +K++ADC EA++G LT+ G + +L L L ++P+ + N D+ +I P+ F++ + + I+ + EK +NY FKNK Y++QA TH S N CYQRLEFLGD+++D+L+T ++ + ++ +PG +T+LRSALVNN FA L+VK+ +HKY ++FH++ V+ +L N++Q Sbjct: 1546 IADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPEVRKTNTDLSSCKGIDYGYLKIPPRCMFEHPDAEKTLDHLISGFENFEKKINYPFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAISPELFHVIDDFVQFQLEKNEMQ 1745 HSP 3 Score: 61.2326 bits (147), Expect = 1.925e-42 Identity = 28/60 (46.67%), Postives = 40/60 (66.67%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQ 4112 V K +GDIFES+AGAI++DS L+ VW VY M+ ++KF N+PR+ + L E P+ Sbjct: 1766 VPKAMGDIFESLAGAIYMDSGMSLETVWHVYYPMMQPLIEKFSANVPRSPVRELLEMEPE 1825 HSP 4 Score: 71.633 bits (174), Expect = 1.186e-11 Identity = 39/90 (43.33%), Postives = 56/90 (62.22%), Query Frame = 2 Query: 3047 NDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVV---KNSDE 3307 +D NLE+ E+LGDSFLK + YLF D +EG+L+ RS+ +SN NL+ L +KG + F+P N+LPP ++V KNSD+ Sbjct: 1305 SDGFNLERLEMLGDSFLKHAITTYLFCTYPDA-HEGRLSYMRSKKVSNCNLYRLGKKKGSPSRMVVSIFDPPVNWLPPGYIVNQDKNSDK 1393
BLAST of Endoribonuclease Dicer vs. Ensembl Mouse
Match: Dicer1 (dicer 1, ribonuclease type III [Source:MGI Symbol;Acc:MGI:2177178]) HSP 1 Score: 228.794 bits (582), Expect = 7.994e-60 Identity = 269/1062 (25.33%), Postives = 486/1062 (45.76%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDYLN-VKSD-----KKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIARE-FCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSND-NSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKS--DMLMCSEKCKKLINLILDLKS--------------KNED------------------------------FKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQ-YPKKLQMSV--TKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVF--THETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSERNVTPSEDDKYDYVPY----GENGPRITLNSASSVLNRYLGKLRCNSISE--PKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNR--------------FDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFL--KLGINDISMF---DVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKF-EYNLPVNSINYSLLLKCHKILFNIYSERIRRFDKNIF-FDPSKAKSS---IILTILKDNK---IDFNKIKEIITNPIVDNLIKNDQSS----------WVGSVIRPNYIT-NEFGAFVISKVCHDMNADSIFKND--MTYEKFLNKKYNIILSPNEKLSPLIKV------IRLTTIQSCVNKERRFLRNNRKLQRINESECY-----FVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDF 2884 R YQ+E+ A + N I+ LNTGSGKTFIA +L K+ + ++ D K+ FLV +++ V Q ++++ + LK +G + N+ +WTK++WS++ +K+++++MT V L +L + +LS + L+FDE H Y +I + S +ILGLTA ++ G +++E +++LE L T+ D + + + E +++ F D +++ + ++E+ + ND N + E+++ K+ LS+ +VL LG + M + +L + ++ K L+ D L + I++ E Y S + D+ + K KL+ ++ K +N+D F N + +IF+ + + LN L+ K + +I+SNFI GH + P+ QM KQ E+L+ F+ + N+LIAT+V+EEG+D+ CN+VV+FD P ++RSY+QSKGRARA S Y++ T + + E ++ + I KIL+++ + + D +E +V DD+ + PY + GPR+T+N+A +NRY +L + + PK D + +L LP+ +P I G P + R A++ V L C+ LHK ELD L+P + + ++ + + Y K L + P YLY I + L +L ++ D + GIL PI Q FP+Y G ++ I+ + ++ L+ + H+ +F+ I R +K F P+ A+S+ + L ++ D+ IDF +++I + + S + +VI P Y ++ F ++ V D+ S F + T+ ++ KYN+ L+ + PL+ V + L T + +N++ + L + +R + E VP L + +SLW + P+I +R++ +L+ E+ Sbjct: 35 RKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELAHQIRGDLNPHAKRTVFLVNSANQVAQQVSAVRTHSDLK--VGEYSDLEVNA---SWTKERWSQEFTKHQVLIMTCYVALTVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCESCPSCPRILGLTASILNGKCDPEELEEKIQKLERILRSDAETATDLVVLDRYTSQPCEIVVD--CGPFTDRSGLYERLLMELEAAL----DFINDCNVAVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTLL---RKIHALCEEYFSPASLDLKYVTPKVMKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRSKQMEAEFRKQEEVLRKFRAHETNLLIATSVVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAI----EKILRNKCSK---SADGAEADVHAGVDDEDAFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPD--GTFYSTLYLPINSPLRASIVGPPMDSVRLAERVVALICCEKLHKIGELDEHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPK---PDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQ---IPHFPVYTRSGEVTISIELKKSGFTLSQQMLELITRLHQYIFS----HILRLEKPALEFKPTGAESAYCVLPLNVVNDSGTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQ--PLLDVDHTSSRLNLLTPRH-LNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEEL 1060 HSP 2 Score: 123.635 bits (309), Expect = 2.084e-39 Identity = 95/321 (29.60%), Postives = 153/321 (47.66%), Query Frame = 2 Query: 3131 SNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKY-------FCYTNFNPKYNYLPPNFVVK---NSDESVEVNNSREFI--------LIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNL-----------------------------------PLVPDNELNADIE-----ISPKLAFQNISMDTDIPQSINDL-------EKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE-ELLNNQLQ 3895 ++D+ E K+ +K S L + GL+ + CY +P +FVV S+E+ V+ ++ I I +K++ADC EA++G LT+ G + +L L L P+ P + D+E I P+ F + D +++N L EK +NY FKNK Y++QA TH S N CYQRLEFLGD+++D+L+T ++ + ++ +PG +T+LRSALVNN FA L+VK+ +HKY ++FH++ V+ +L N++Q Sbjct: 1460 ASDSAYEWKMPKKAS-------LGSMPFASGLEDFDYSSWDAMCY--LDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTSREKALDPAQENGSSQQKSLSGSCASPVGPRSSAGKDLEYGCLKIPPRCMFDH----PDAEKTLNHLISGFETFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVKFQLEKNEMQ 1767 HSP 3 Score: 60.8474 bits (146), Expect = 2.084e-39 Identity = 28/60 (46.67%), Postives = 40/60 (66.67%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQ 4112 V K +GDIFES+AGAI++DS L+ VW VY M+ ++KF N+PR+ + L E P+ Sbjct: 1788 VPKAMGDIFESLAGAIYMDSGMSLEVVWQVYYPMMQPLIEKFSANVPRSPVRELLEMEPE 1847 HSP 4 Score: 80.8777 bits (198), Expect = 1.328e-14 Identity = 45/113 (39.82%), Postives = 66/113 (58.41%), Query Frame = 2 Query: 3047 NDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVV---KNSDESVEVNNSREFILIKNKNLADCCE 3376 +D NLE+ E+LGDSFLK + YLF D +EG+L+ RS+ +SN NL+ L +KGL + F+P N+LPP +VV K++ E E + + L+ N L + CE Sbjct: 1297 SDGFNLERLEMLGDSFLKHAITTYLFCTYPDA-HEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNSEKWEKDEMTKDCLLANGKLGEACE 1408
BLAST of Endoribonuclease Dicer vs. Ensembl Mouse
Match: Dicer1 (dicer 1, ribonuclease type III [Source:MGI Symbol;Acc:MGI:2177178]) HSP 1 Score: 96.2857 bits (238), Expect = 3.171e-20 Identity = 94/357 (26.33%), Postives = 184/357 (51.54%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDYLN-VKSD-----KKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIARE-FCGNIKETIINYETESFPDLVAIFKSAIEKIES---FIPVDGHHSNDNSGSLNAEQNNFKTL-KKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKS--DMLMCSEKCKKLINLI 1078 R YQ+E+ A + N I+ LNTGSGKTFIA +L K+ + ++ D K+ FLV +++ V Q ++++ + LK +G + N+ +WTK++WS++ +K+++++MT V L +L + +LS + L+FDE H Y +I + S +ILGLTA ++ G +++E +++LE L T+ D + + + E +++ F D +++ + ++E+ FI ND + ++++++ + + K+ LS+ +VL LG + M + +L + ++ K L+ D L+ I++ E Y S + D+ + K KL+ ++ Sbjct: 35 RKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELAHQIRGDLNPHAKRTVFLVNSANQVAQQVSAVRTHSDLK--VGEYSDLEVNA---SWTKERWSQEFTKHQVLIMTCYVALTVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCESCPSCPRILGLTASILNGKCDPEELEEKIQKLERILRSDAETATDLVVLDRYTSQPCEIVVD--CGPFTDRSGLYERLLMELEAALDFI-------NDCNVAVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTL---LRKIHALCEEYFSPASLDLKYVTPKVMKLLEIL 374
BLAST of Endoribonuclease Dicer vs. Ensembl Mouse
Match: Drosha (drosha, ribonuclease type III [Source:MGI Symbol;Acc:MGI:1261425]) HSP 1 Score: 84.3445 bits (207), Expect = 1.047e-15 Identity = 73/270 (27.04%), Postives = 124/270 (45.93%), Query Frame = 2 Query: 3065 EQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGIDYSIQYLQ------------WLNLPLVP--DNELNAD---IEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANR-KFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKY 3820 E+ E LGD+ ++F+TS +L++ + EG L R+ ++ N++L LA + LD++ Y + P+ + + SD +A+C EA+IG++ ++ + Q WLN PL P E N D IE SP L Q + + E+ + F + + +A T + N QR+EFLGDS++ + T Y+ ++ + G +T LRS+LVNN T A ++ + G +Y Sbjct: 965 ERLEFLGDAVVEFLTSVHLYYLFPSLE-EGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHG--------PD-LCRESD--------------LRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVL------------QKLTEFEEAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEY 1198
BLAST of Endoribonuclease Dicer vs. Ensembl Mouse
Match: Drosha (drosha, ribonuclease type III [Source:MGI Symbol;Acc:MGI:1261425]) HSP 1 Score: 84.3445 bits (207), Expect = 1.047e-15 Identity = 73/270 (27.04%), Postives = 124/270 (45.93%), Query Frame = 2 Query: 3065 EQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGIDYSIQYLQ------------WLNLPLVP--DNELNAD---IEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANR-KFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKY 3820 E+ E LGD+ ++F+TS +L++ + EG L R+ ++ N++L LA + LD++ Y + P+ + + SD +A+C EA+IG++ ++ + Q WLN PL P E N D IE SP L Q + + E+ + F + + +A T + N QR+EFLGDS++ + T Y+ ++ + G +T LRS+LVNN T A ++ + G +Y Sbjct: 965 ERLEFLGDAVVEFLTSVHLYYLFPSLE-EGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHG--------PD-LCRESD--------------LRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVL------------QKLTEFEEAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEY 1198
BLAST of Endoribonuclease Dicer vs. Ensembl Mouse
Match: Ddx58 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 [Source:MGI Symbol;Acc:MGI:2442858]) HSP 1 Score: 70.0922 bits (170), Expect = 1.873e-11 Identity = 121/543 (22.28%), Postives = 230/543 (42.36%), Query Frame = 2 Query: 32 SFMKTRDYQIEIYNKAK-NENVIIKLNTGSGKTFIASMLIKDYLN---VKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKY-NLSQVCYLIFDEVHHLTKSDDYSQIS----EHVIRTTSDI--KILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIAREFCGNIKETIINYETESFPDLVA-----IFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLV-------PCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLK-FINSNIESYISKSDMLMC-----------------SEKCKKLINLILDLKSKNEDFKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQYPKKLQMSVTKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHETQKTEAQIQKFNLINEEL 1537 S +K R+YQ+E+ AK +N II TG GKTF++ ++ + +L K+ F + Q Y + +GY + + D+ I II++T ++ ++ LN+ +LS +IFDE H+ +K+ Y+QI +H + + D +++GLTA + GV K E A++ + KL + S+ R+ +++ + Y+ + VA FK I ++ ++ G L QN +KY V + ++ +F+ H ND++ E ++ + L+ LK F + E+ +++ + S + KL +L L L+ + K ++F+ + V AL + + N A F+ + G + + + K + NILIAT+V +EG+D++ CN+V+ ++ + +Q++GR RA++S+ + T E +K N+I E++ Sbjct: 240 SPLKPRNYQLELALPAKKGKNTIICAPTGCGKTFVSLLICEHHLKKFPCGQKGKVVFFANQIPVYEQQATVFSRYF---ERLGYNIASISGATSDS---VSVQHIIEDNDIIILTPQILVNNLNNGAIPSLSVFTLMIFDECHNTSKNHPYNQIMFRYLDHKLGESRDPLPQVVGLTASV--GVGDAKTAEEAMQHI-CKLCAALDASVIATVRDNVAELEQVV--YKPQKISRKVASRTSNTFKCIISQLMKETEKLAKDVSEELGKLFQIQNREFGTQKYEQWIVGVHKACSVFQMADKEEESRVCKALFLYTSHLRKYNDALIISEDAQM--TDALNYLKAFFHDVREAAFDETERELTRRFEEKLEELEKVSRDPSNENPKLRDLYLVLQEEYH-LKPETKTILFVKTRALVDALKKWI----EENPALSFLKPGILTGRGRTNRATGMTLPAQK-CVLEAFRASGDNNILIATSVADEGIDIAECNLVILYEYVGNVIKMIQTRGRGRARDSKCFLLTSSADVIEK--EKANMIKEKI 761
BLAST of Endoribonuclease Dicer vs. UniProt/SwissProt
Match: sp|P84634|DCL4_ARATH (Dicer-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=DCL4 PE=1 SV=2) HSP 1 Score: 260.381 bits (664), Expect = 7.337e-74 Identity = 351/1393 (25.20%), Postives = 618/1393 (44.36%), Query Frame = 2 Query: 44 TRDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDYLN-VKSDKK--IFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDD--YSQISEHVIRTTS--DIKILGLTACLI--KGVKHVKDIEFAVKQLEHKLNCKVITSMDNIA---------------REFCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFS----VLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLS-CLKFINSNIESYISKSDMLMCSEK-----CKKLINLILDLKSKNEDFKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQYPKKLQMSVTKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHETQKTEAQ-IQKFNLINEELNKILKSRVIEDELNKDDSERNVTPSEDDKYDYVPYGENGPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYFQ-DEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNRFD-NNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFLKLGINDISMFDVSTYLGILLPNPIHQNKD----FMKFPIYLVGGMTSVEIKFEYNLPV--------------NSINYSLLLK--CHKILFNIYSERIRRFDKNIFFDPSK-AKSSIILTI---LKDNK----IDFNKIKEIITNPI-------VDNL-------IKNDQSSWVGSVIRPNYITNEFGA--FVISKVCHDMNADSIFKNDMT--YEKFLNKKYNIILSPNEKLSPLIKVIRLTTIQSCVNKERRFLRNNRKLQRINESECYFV---PSLT--ECISLSSSLWTQLCIFPTIHHRMNQILSIEDFAHDLEFMNLIENSDCFEINFDDCYNIENIESSIYDPNVLKFINN-IFPCFMAKTCNDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPK--YNYLPPNFVVKNSDESVEVNN-SREFILIKN----------------KNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPDNELNADIEISPKLAFQNISMDTDIPQS----INDLEKILNYHFKNKGYIVQAITHPSSIANR-KFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVEELLN 3883 R YQ+E+ KA ENVI+ L TG GKT IA MLI + + V S KK FL +LV Q + D + K I + G+ W ++ + ++ VMT ++ L L H + + LIFDE HH + + Y++I + ++ S +I G+TA + KG +++ ++ LE+ LN KV + N+ R + ++ I YE L I + + ++ I D H + ++K+ L S L LGL + + H V ++ + + K+K+ D +LS + ++S + + SD+L + +KL+ LI L + + +IF+N + L+ +L L + S+F++G S+ MS ILK F+ K+ N+L+AT V EEGLD+ C +V+++D P+ S++QS+GRAR S+Y + E I+ F + + +N + R E+ + D E E G I+ S+ S+L +Y +L + +PK + + DEF + LP AP + EI+ + AK+ L+A LH L+ LLP ++ + + + + D + IK E + L + +P L+ K+ PS S ++ +Y + +P+P + FMK P+ + +++ + V + I + L + K+LF E I F D S+ +KS+ L + L D + +D+ I+ +++PI V+++ +K W ++ + + + + ++ +CH N S K T + + + K Y + L PL++V L +++ ++ ++ N + Q ++E YF+ P L+ + LS + + L + P+I HRM +L + H L +SI P + + + + + C++R +LE+ E+LGD+FLKF S++LF +D+ +EG+LT++RS +++N NL LA +K L Y +P + + P V + S EV++ +R+ ++++ K +AD EA++G+ L + G ++++L+W+ + N D E S ++ I+ +P + + LE L+Y F +KG +VQA HPS NR CYQRLEFLGD+V+D+L+T Y KL PG++T+LRS VNN A ++V F ++L + +S H +V+E+ N Sbjct: 124 ARRYQVELCKKATEENVIVYLGTGCGKTHIAVMLIYELGHLVLSPKKSVCIFLAPTVALVEQQAKVIADSVNFKVAI-HCGGKRIVKSHSEWEREIAANEVL-----VMTPQILLHNLQHCFIKMECISLLIFDECHHAQQQSNHPYAEIMKVFYKSESLQRPRIFGMTASPVVGKGSFQSENLSKSINSLENLLNAKVYSVESNVQLDGFVSSPLVKVYYYRSALSDASQSTIRYENM----LEDIKQRCLASLKLLI--DTHQTQ-----------TLLSMKRLLKRSHDNLIYTLLNLGLWGAIQAAKIQLNSDHN-VQDEPVGKNPKSKI-CDTYLSMAAEALSSGVAKDENASDLLSLAALKEPLFSRKLVQLIKILSVFR--LEPHMKCIIFVNRIVTARTLSCILNNLELLRS----WKSDFLVGLSSGLK----SMSRRSMETILKRFQSKELNLLVATKVGEEGLDIQTCCLVIRYDLPETVTSFIQSRGRARMPQSEYAFLVDSGNEKEMDLIENFKVNEDRMNLEITYRSSEETCPRLDEE------------LYKVHETGACISGGSSISLLYKYCSRLPHDEFFQPKPEFQFKPVDEFGGTICRITLPANAP-ISEIESSLLPSTEAAKKDACLKAVHELHNLGVLNDFLLPDSKDEIEDELSDDEFDFDNIKGEGCSRGDL----YEMRVPV----LFKQKWDPST---------SCVNLHSYYIMFVPHPADRIYKKFGFFMKSPLPVEAETMDIDLHLAHQRSVSVKIFPSGVTEFDNDEIRLAELFQEIALKVLFE-RGELIPDFVPLELQDSSRTSKSTFYLLLPLCLHDGESVISVDWVTIRNCLSSPIFKTPSVLVEDIFPPSGSHLKLANGCWNIDDVKNSLVFTTYSKQFYFVADICHGRNGFSPVKESSTKSHVESIYKLYGVEL--KHPAQPLLRVKPLCHVRNLLHNR---MQTNLEPQELDE---YFIEIPPELSHLKIKGLSKDIGSSLSLLPSIMHRMENLLVAIELKHVLS-------------------------ASI--PEIAEVSGHRVLEALTTEKCHERLSLERLEVLGDAFLKFAVSRHLF-LHHDSLDEGELTRRRSNVVNNSNLCRLAIKKNLQVYIRDQALDPTQFFAFGHPCRVTCDEVASKEVHSLNRDLGILESNTGEIRCSKGHHWLYKKTIADVVEALVGAFLVDSGFKGAVKFLKWIGV--------NVDFE-SLQVQDACIASRRYLPLTTRNNLETLENQLDYKFLHKGLLVQAFIHPS--YNRHGGGCYQRLEFLGDAVLDYLMTSYFFTVFPKLKPGQLTDLRSLSVNNEALANVAVSFSLKRFL-FCESIYLH---EVIEDYTN 1399 HSP 2 Score: 42.3578 bits (98), Expect = 7.337e-74 Identity = 19/42 (45.24%), Postives = 28/42 (66.67%), Query Frame = 3 Query: 3939 KVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFN 4064 KVLGD+ ES GA+F+D ++L VWT+ F+ +P+K N Sbjct: 1418 KVLGDLVESCLGALFLDCGFNLNHVWTMMLSFL-DPVKNLSN 1458
BLAST of Endoribonuclease Dicer vs. UniProt/SwissProt
Match: sp|A2RAF3|DCL1_ASPNC (Dicer-like protein 1 OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=dcl1 PE=3 SV=2) HSP 1 Score: 229.565 bits (584), Expect = 1.726e-61 Identity = 329/1351 (24.35%), Postives = 555/1351 (41.08%), Query Frame = 2 Query: 38 MKTRDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIK--------DYLNVKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTT-------SDIKILGLTACLI--KGVKHVKDIEFAVKQLEHKLNCKVITSMD-NIAREFCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIE-SYISKSDMLMCSEKCKKLINLILDLKSKNEDF---KNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQYPKKLQMSVTKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHETQKTEAQIQKFNLINEELNKILKSRVIEDELNKD-DSERNVTPSEDDKYDYVPYGENGPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSL-------LPYNRFDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFLKLGINDISMFDVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFEYNLPVNSINYSLLLKCHKI-LFNIYSERIRR------FDKN-----IFFDPSKAKSSIILTILKDNKIDFNKIKEIITNPIVDNLIKNDQSSWVGSVIRPNYITNEFGAFVISKVCHDMNADSIFKNDMTYEKFLNKKYNIILSPNEKLSPLIKVIRLTTIQSC-----------VNKERRFLRNNRKLQRINESECYFVPSLTECISLSSSLWTQLCIFPTIHHRMNQ-ILSIEDFAHDLEFMNLIENSDCFEINFDDCYNIENIESSIYDPNVLKFINNIFPCFMAKTCNDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNF-VVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGIDY----SIQYLQWL-NLPLVPDNELNADIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPS-SIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVEELLNNQLQCEHS 3907 + R+YQ+E++ +AK +N I L+TGSGKT IA +L+K D + K + FFLV + +L +Q L++ L +G+F+G M D W+K W + K +IV TA++ L ++ +SQ+ LIFDE HH K Y++I IR + +I G+TA I KG DI +LE L+ ++ T+ + ++ R+ E + ++ P +++K H D G + + F+ + SE S + + D + L G+V + + +T + E S K I E S I KS E +L + L+ + + + ++F ++ L L L L F+ +IG + + ++ +Q L F+ + N L AT+V EEGLD+ CN+VV+FD Q Y+QS+GRAR NS Y E E + + + + E+ + + ED L D + + +D+ G ++T +SA+++L RY L+ ++ Y F ++LP +P + + G P K AK+ + C +L K LD LP R + L I +Y+ + L + Q + P +L+ I + PS+ L + +F LP+ F +FPI+L + E + + LLL ++ +++ R+ R +DK + P+K +SS + + +D+ + + N + D SWV + + + F V H + S + + +N N LS L K R + +C V R L +R E+ C+ + ++ +S FP I R++ ++S++ E +N F + D +S D + + I+ + R N E+ E LGD FLK TS LF N D+E R LI NKNLF+ A K + KY F+ ++ + P +++ D S + + L + K +AD CEA+IG+ L + G D+ +++ + L N P I +Q + D +E+ L+Y F+ + A THPS A K CYQRLEFLGDS+ID + + +P +T + A+V+N L+VK G H +L YF + + + + EE+ + + E + Sbjct: 115 LDPREYQLELFERAKVQNTIAVLDTGSGKTLIAVLLLKHTLEKELNDRMEGKPHRIAFFLVDSVTLAYQQAAVLRNNL--DQSVGHFFGAMGT---DLWSKSVWDQHFQKNMVIVCTAEILNQCLLNSYIKMSQINILIFDEAHHTKKDHPYARI----IRDSYLEEVYSKRPRIFGMTASPIDTKG-----DIVDEATRLEKLLDSRIATTSNMSLLRQVARRPVERVWSFNKLEQPFATSLYK---------------HLEDRFGDMACLEGIFRFAWQASSELGRWCSDRAWA--RALADDVLPKLEGSVRKTA---NSETSSNVPE--SAYKEILRITEASEIVKSYAFSSPETFGQLSPKVQVLREELARYFGRQTETKCIVFTQKRYTALILAELFQTL-----NIPFLRPGVLIGVRSGDL-AGMNITFRQQFISLVKFRTGEINCLFATSVAEEGLDIPDCNLVVRFDLYQTLIQYVQSRGRARHFNSTYASMV-ERGNLEHEQRLLEVQDAEMMQSFCRTLPEDRLLYGFDHDLDTVLQKDEGNRTFRIKSTGAKLTYHSATAILARYASSLQYEKEFSAQVTYVVLPINGAFV-CEVILPEKSP-IRGLTGSPAMKKSIAKRSAAFDTCLLLRKNKLLDDHFNSIYHRRLPAMR-NAKLAITSKR---TSQYDMISKPSLWGRKQGM--PPKELHGTFITFLPSMQLSHEPAPLLLFTRER-----LPH-------FPEFPIFL-------DDDVETTIITTPLEKQLLLSEKEVDALTVFTLRVFRDVFHKTYDKEPEKMAYWLAPAKVQSSYLPSYDPRQILDWESLTYVRDNDSIPFSTNADPESWVDLFVFDAW--DGRCRFFTVGVEHSLTPSSPPPPFVARRRHMNDVMNYCLS-------LSKNSRAKFLSTCHWDQPVLRAELVRLRRNLLDKMTDTERDVETRCFICIEPLKVSAIPASTAFSCLAFPAIISRIDAYLISLQG----CESLN-------FTVKLDLALEAFTKDSDNTDEHRAQQIH-------VQRGMGR-NYERLEFLGDCFLKMATSIALFT-QNPDDDEFDYHVNRMCLICNKNLFNAAVDKEIYKYIRSRGFS-RHTWYPEGLKLLQGKDHSRKATTESKHALAE-KTIADVCEALIGAALLSGGPDHRFDMAVKAVTTLVNSPSHKAERWKDYISFYTIPKYQRRAADGAELYLSRKIEEKLSYRFRYPTLLGSAFTHPSYPSAWAKVPCYQRLEFLGDSLIDMVCVEDLFARFPDRDPQWLTEHKMAMVSNKFLGALAVKLGLHTHLKYFSAPLQSQITQYAEEIQTAEGESEGA 1364 HSP 2 Score: 31.5722 bits (70), Expect = 1.726e-61 Identity = 15/44 (34.09%), Postives = 27/44 (61.36%), Query Frame = 3 Query: 3939 KVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNI 4070 K L D+ E+ GA+FVDS+++ + V + F + +K FF ++ Sbjct: 1376 KCLPDMVEAYVGAVFVDSDFNFE----VIERFFRDYIKPFFEDM 1415
BLAST of Endoribonuclease Dicer vs. UniProt/SwissProt
Match: sp|Q4WVE3|DCL1_ASPFU (Dicer-like protein 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=dcl1 PE=3 SV=3) HSP 1 Score: 221.861 bits (564), Expect = 1.605e-60 Identity = 327/1359 (24.06%), Postives = 548/1359 (40.32%), Query Frame = 2 Query: 32 SFMKTRDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIK--------DYLNVKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQI---SEHVIRTTSDIKILGLTACLI--KGVKHVKDIEFAVKQLEHKLNCKVITSMD-NIAREFCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDN--SGSLNAEQNNFKTLKKYLSE-------SFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKSDMLMCSEKCKKLINLILDLKSKNEDFKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQYPKKLQMSVTKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTH----ETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSERNVTPSEDDKYDYVPYGENGPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNRFDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFLKLGINDISMFDVSTYLGILLPNPIHQN------------KDFMKFPIYL----VGGMTSVEIKFEYNLPVN---SINYSLLLKCHKILFNIYSERIRRFDKNIFFDPSKAKSSIILTILKDNKIDFNKIKEIITNPIVDNLIKNDQSSWVGSVIRPNYITNEFGAFVISKVCHDMNADSIFKNDMTYEKFLNKKYNIILSPNEKLSPLIKVIRLTTIQSC-----------VNKERRFLRNNRKLQRINESECYFVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDFAHDLEFMNLIENSDCFEINFDDCYNIENIESSIYDPNVLKFINNIFPCFMAKTCNDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLV--PDNELNADIE-----ISPKLAFQ-NISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPS--SIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVEELLNNQLQCEHS 3907 + R+YQIE++ +AK +N I L+TGSGKT IA +L++ D N K+ + FFLV + +L +Q L++ + + +F+G M D W K W K + + +IV TA++ L ++ + Q+ LIFDE HH K Y++I S + + ++ G+TA I KG DI A +LE L+ ++ T+ + RE E + Y P F + + K+ +D + N G N L K+ S+ + VL L KLI ++ HGAV D + E ++ + F + D + E K+ L ++L D T I +F ++ LN L +L N + +IG + ++ +Q L F+ + N L AT+V EEGLD+ CN+V++FD + Y+QS+GRAR S Y + E + +I++ I +IL I L+ +D + + E++ G ++T +SA ++L RY L+ + P++ Y N + ++LP +P + + G P K AK+ + C +L K N L+ N I Y + + K L+I + Y + PS++ K F ++ +L P+ ++ +F F IYL + S +K + V+ S+ L I +Y +++ + P++A + ID++ + + N + + S V I N+ F F ++ V + + +++N N LS L K R + SC V R L ++ ++EC+ +L S+ + FP I R++ L + +LE +I E D N E + + F M K N E+ E LGD FLK TS LF N D+E R LI NKNLF+ A +K + +Y F+ Y ++ D S ++ + + L K +AD CEA+IG+ L + G ++ LV P + ++ E PK + + + D+ + + E+ L YHF + AITHPS S + CYQRLEFLGDS++D + + +P ++ + A+V+N LSVK GFH+ + F + + + VEE+ Q + + + Sbjct: 122 GMLDPREYQIELFERAKTQNTIAVLDTGSGKTLIAVLLLRHTILNELDDRANGKTHRVSFFLVDSVTLAYQQAAVLRNNI--DQNVAHFFGAMG---TDLWDKRTWDKHLQRNMVIVCTAEILNQCLLNSYVRMDQINLLIFDEAHHAKKDHPYARIIRDSYFKAQPSQRPRVFGMTASPIDTKG-----DITEAATRLETFLDSRIATTSKITLLREVVSRPIEKVWAYNRLEPP-----FATELYKL-----MDTRYGNIKVLEGVYRFAWNASSELGKWCSDRAWWHALADDVLPKLEGNINKLIESNTMKAEHGAVFKDIIRIREASETVKNYFFT----------------DPELPGELSPKVQRLRMELSKHFNDTTGTKCI-VFTQKRYTAKILNELFTVLNIPN-----LRPGVLIGVRPGDI-GGMNITFRQQFLALVKFRTGEINCLFATSVAEEGLDIPDCNLVIRFDLYRTLIQYVQSRGRARHCTSTYAIMVEKDNAEHEGRLKEIREAENIMRRFCEILPEDRI---LHGNDHDLDSFLQEEEGRRTFTVKSTGAKLTYHSAIAILARYASSLQYEKETVPQVTYV-VTIASNAYVCEVILPEKSP-IRGLTGSPAMRKAVAKRSAAFDTCLLLRK---------------NRLLDGYFNSI----YHRRLPAMRNAK---LAITCKRTNAYDMLLKPSIWAKQRTTPTETF-YGIHMSLLPSKPLSRDHRPILLLTREKLPEFPAFSIYLDEDVETKVLSYPLKHGLQISVDKLQSLTVFTLRIFRDIFHKVYEHEVQKMP--YWLAPAEAIDGRGSGKNPRDCIDWDTVSFVHNNDEITFTRNLNPESLVNRFIFDNW-DGRFRYFTVA-VADTLQPSDPPPPSVPRRRYMNNIMNYTLS-------LSKNSRARFLSSCDWNQPVLQAELVRLRRNLLDKMTTQEKEMQTECFICAEPLRISALPPSIVSTCLAFPAIISRLDSYLIALEACDELEL--VIRPDFALEAFTKDSDNTEE-----HRGQQIHFQRG-----MGK------NYERLEFLGDCFLKMATSIALFS-QNPNDDEFDYHVNRMCLICNKNLFNTAIKKQIYRYIRSRGFSRHIWYPDGLTLLHGKDHSTKLLSEGKHAL-GEKTIADVCEALIGASLLSGGPEHRFDMATKAVSALVDSPSHRVSCWKEYITLYTLPKYQTEKHRGSEDDLARHV---EEELGYHFTYPRLLASAITHPSLPSTWGYRVPCYQRLEFLGDSLLDMVCVEDLFRRFPDRDPQWLSEHKMAMVSNKFLGALSVKLGFHRRIMAFSNPLQAQITHYVEEIETAQAESQGA 1375 HSP 2 Score: 36.1946 bits (82), Expect = 1.605e-60 Identity = 21/62 (33.87%), Postives = 32/62 (51.61%), Query Frame = 3 Query: 3939 KVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIP-------RNKISMLHEK 4103 K L D+ E+ GAIFVDS +D + V + F E +K FF ++ ++ + LH K Sbjct: 1387 KCLPDMVEAYLGAIFVDSKFDFQ----VIEAFFERQIKPFFEDMSIYDTFANKHPTTFLHNK 1444
BLAST of Endoribonuclease Dicer vs. UniProt/SwissProt
Match: sp|A1CBC9|DCL1_ASPCL (Dicer-like protein 1 OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) OX=344612 GN=dcl1 PE=3 SV=2) HSP 1 Score: 225.328 bits (573), Expect = 5.124e-60 Identity = 318/1374 (23.14%), Postives = 567/1374 (41.27%), Query Frame = 2 Query: 32 SFMKTRDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIK--------DYLNVKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQI---SEHVIRTTSDIKILGLTACLI--KGVKHVKDIEFAVKQLEHKLNCKVITSMD-NIAREFCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCK------LITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFI----NSNIESYISKSDMLMCSEKCKKLINLILDLKSKNEDFKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQYPKKLQMSVTKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTH-ETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSERNVTPSEDDKYDYVPYGENGPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNRFDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFLK-LGINDISMFDVSTYLGILLPNPI---HQN---------KDFMKFPIYLVGGMTSVEIKFEYNLPVNSINYSLLLKCHKI-LFNIYSERIRRFDKNIFFDPSKAKSSIILTILKDNK-IDFNKIKEIITNPIVD----NLIKNDQSSWVGSVIRPNYITNEF--------GAFVISKVCHDMNADSIFKNDMTYEKFLNK--KYNIILSPNEKLSPLI------KVIRLTTIQSCVNKERRFLRNNRKLQRINESECYFVPSLTECISLSSSLWTQLCIFPTIHHRMNQ-ILSIEDFAHDLEFMNLIENSDCFEINFDDCYNIENIESSIYDPNVLKFINNIFPCFMAKTCNDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGIDYSI-------------------QYLQWLNLPLVPDNELNADIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANRKF--SCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVEELLNNQLQCEHS 3907 + R+YQIE++ +AK +N I L+TGSGKT IA +L++ D N K + FFLV + +L +Q L++ + + +F+G M D W + W + + + +IV TA++ L ++ ++Q+ LIFDE HH K Y++I S +I G+TA I KG DI A +LE L+ ++ T+ + R+ E + Y+ P FK+ + K+ + N K L+ ++ S LG C + D + L G VN E T + ++ + SNI + +D E K+ L +L SK+ +IF ++ L+ L IL + + +IG + + ++ +Q L F++ + N L AT+V EEGLD+ CN+VV+FD Y+QS+GRAR +S Y + EA++++ + + ++ + L+ +D + + ++ G R+T +SA ++L RY L+ + P+ Y Q N + ++LP +P + + G P K AKQ + C +L ++ LD + + I +++ A + + L+I + K Y + PS++ + GI ++ T L +L P+ HQ DF FPIYL + E + S++ + L ++ ++ RI R ++F + +S + L + +D N +E I+D ++ + + P + N F F KV + A + + + ++ Y + LS N + L V++ ++ N + +K+Q +EC+ + ++ S+ + FP I R++ ++SIE + ++L+ SD Y +E + + + F M K N E+ E LGD FLK TS LF N D+E R LI NKNLF+ A ++ + +Y +F+ ++ + P + + + S+ + K +AD CEA+IG+ L + G ++ ++ +++ L P+ ++ LA Q +E+ L YHF+ + A+THPS+ + F CYQRLEFLGDS++D + + +P +T + A+V+N L+VK GFHK++ F + + + VEE+ + + E + Sbjct: 119 GLLDPREYQIELFERAKAQNTIAVLDTGSGKTLIAVLLLRHVLQNELNDRANGKPHRVSFFLVDSVTLAYQQAAVLRNN--IDQNVAHFFGAMG---TDLWDRQVWEEHLQQNMVIVCTAEILNQCLLNSHVRMNQINLLIFDEAHHTKKDHPYARIIRDSYFKASPPQRPRIFGMTASPIDTKG-----DIIAAATRLETLLDSRIATTSKITLLRQVVSRPIEKVWAYDRLESP-----FKTNLHKL-----------------MENRFGNVKALEGVFRFAWYASSELGRW-CSDRAWLYALADDVLPKLEGHVNK----LAESTAAATERDMAFKEITLIKEASNIVKAHTFNDPEFPGELSPKVRLLQTEL-SKHFSHAPETKCIIFTQKRYTAKTLHELFTIL-----SIPHLRPGVLIGVRSGDI-GGMNITFRQQFLALVKFRKGEINCLFATSVAEEGLDIPDCNLVVRFDLYHTLIQYVQSRGRARHYHSTYASMVEKDNSDHEARLREVREAEKTMQNFCETLPEDRILHGNDHDLDSLLQHEEGRRTFTVKSTGARLTYHSAIAILARYASSLQYEKETTPQATYV-VQSVGNTYVCEVILPEKSP-IRGLTGSPAIRKSIAKQSAAFDTCLLLRRHKLLD------------------DFFKSIYHKRLPAMRNA----KLAITSKKTNQYDMLLKPSIWRRHQGILPSKLY--GTILSLLPSEPLSREHQPILVLTREKLPDFPAFPIYL-------DEDIETKVVPRSLDTGMELSAEELRALTTFTLRIFR---DVFHKVYEQESEKLPYWLAPAEPLDANG-REPGPRGIIDWKTVTFVQENDEIVFSRDLAPESLVNRFMFDKWDGRSRFFTIKVMEGLRAADPPPSSVPRRRHMDNIMSYCLSLSKNSRARFLAGCHWDQPVLQAELVRLRRNLLDKLTTEEKKIQ----TECFICAEPLKISAIPPSIASTCLAFPAIITRLDSYLISIE----ACDELDLVIRSD---------YALEAVTKDSDNTEEHRGQQIHFQRGMGK------NYERLEFLGDCFLKMATSIALF-TQNPDDDEFDYHVNRMCLICNKNLFNTAKKRQIYRYIRSRSFS-RHVWYPDGLTLLQGKDHSKKMLSQAKHALGEKTIADVCEALIGACLLSGGPEHRFDMGVKAVSVFVDSPSHAVSRWKEYIGLYKPPNYQVRKAEGAETNLALQ--------------VEEKLGYHFRYPRLLCSAVTHPSTPSTWYFRVPCYQRLEFLGDSLLDMVCVEDLFHRFPDRDPQWLTEHKMAMVSNKFLGALAVKLGFHKHIKAFSNPLQAQITYYVEEIETAEAESEGA 1372 HSP 2 Score: 31.187 bits (69), Expect = 5.124e-60 Identity = 15/44 (34.09%), Postives = 26/44 (59.09%), Query Frame = 3 Query: 3939 KVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNI 4070 K L D+ E+ GAIFVDS++ + V + F + +K +F ++ Sbjct: 1384 KCLPDMVEAYLGAIFVDSDFSFE----VIEAFFQAQIKPYFQDM 1423
BLAST of Endoribonuclease Dicer vs. UniProt/SwissProt
Match: sp|Q6TUI4|DICER_BOVIN (Endoribonuclease Dicer OS=Bos taurus OX=9913 GN=DICER1 PE=2 SV=3) HSP 1 Score: 228.794 bits (582), Expect = 5.230e-59 Identity = 272/1061 (25.64%), Postives = 490/1061 (46.18%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDY-LNVKSD-----KKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIARE-FCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTL-KKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKS--DMLMCSEKCKKLINLILDLKS--------------KNED------------------------------FKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQ-YPKKLQMSV--TKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVF--THETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSERNVTPSEDDKYDYVPY---GENGPRITLNSASSVLNRYLGKLRCNSISE--PKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNR--------------FDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFL--KLGINDISMF---DVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFEYNLPVNSINY-SLLLKCHKILFNIYSERIRRFDKNIF-FDPSKAKSS---IILTILKDNK---IDFNKIKEIITNPIVDNLIKNDQSS----------WVGSVIRPNYIT-NEFGAFVISKVCHDMNADSIFKND--MTYEKFLNKKYNIILSPNEKLSPLIKV------IRLTTIQSCVNKERRFLRNNRKLQRINESECY-----FVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDF 2884 R YQ+E+ A + N I+ LNTGSGKTFIA +L K+ ++ D K+ FLV +++ V Q ++++ + LK +G Y +E S +WTK+KW+++ +K+++++MT V L++L + +LS + L+FDE H Y +I + S +ILGLTA ++ G +++E +++LE L T+ D + + + E +++ F D +++ + ++E + + ND + S+++++ + + K+ LS+ +VL LG + M + +L + ++ K L+ D FL + I++ E + S + D+ + K KL+ ++ K +N+D F N + +IF+ + + LN L+ K + +I+SNFI GH + P+ QM KQ E+L+ F+ + N+LIAT+++EEG+D+ CN+VV+FD P ++RSY+QSKGRARA S Y++ T + + E ++ + I KIL+++ + + D E + P DD + PY E+GPR+T+N+A +NRY +L + + PK D + +L LP+ +P I G P S R A++ V L C+ LHK ELD L+P + + ++ + + Y K L + P YLY I + L +L ++ D + GIL PI Q FP+Y G ++ I+ + + S+ L+ + H+ +F+ I R +K F P+ A S+ + L ++ D+ IDF +++I + + S + +VI P Y ++ F ++ V D+ S F + T+ ++ KYN+ L+ + PL+ V + L T + +N++ + L + +R + E VP L + +SLW + P+I +R++ +L+ E+ Sbjct: 45 RKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLK--VGE-YSNLEVS--ASWTKEKWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVD--CGPFTDRSGLYERLLMELEEAL----NFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFL---RKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAI----EKILRNKCSK---SVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPD--GTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPR---PGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQ---IPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFS----HILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQ--PLLDVDHTSSRLNLLTPRH-LNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEEL 1069 HSP 2 Score: 122.094 bits (305), Expect = 2.528e-38 Identity = 84/262 (32.06%), Postives = 134/262 (51.15%), Query Frame = 2 Query: 3281 NFVVK---NSDESVEVNNSREFI--------LIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVP-----DNE--------------------------------LNADIE-----ISPKLAFQNISMDTDIPQSIN---DLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE-ELLNNQLQ 3895 +FVV S+E+ V+ ++ I I +K++ADC EA++G LT+ G + +L L L ++P D E + D+E I P+ F + D + I+ + EK +NY FKNK Y++QA TH S N CYQRLEFLGD+++D+L+T ++ + ++ +PG +T+LRSALVNN FA L+VK+ +HKY ++FH++ V+ +L N++Q Sbjct: 1523 DFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRAAASGAGYRASVLKDLEYGCLKIPPRCMFDHPEADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQ 1784 HSP 3 Score: 61.2326 bits (147), Expect = 2.528e-38 Identity = 28/61 (45.90%), Postives = 40/61 (65.57%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQM 4115 V K +GDIFES+AGAI++DS L+ VW VY M ++KF N+PR+ + L E P++ Sbjct: 1805 VPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPEI 1865 HSP 4 Score: 64.3142 bits (155), Expect = 9.430e-9 Identity = 40/89 (44.94%), Postives = 55/89 (61.80%), Query Frame = 2 Query: 3047 NDRTNLEQFEILGDSFLKF-ITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDES 3310 +D NLE+ E+LGDSFLK IT+ L D +EG+L+ RS+ +SN NL+ L +KGL + F+P N LPP +VV N D+S Sbjct: 1307 SDGFNLERLEMLGDSFLKHAITTVSLSALILDA-HEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNGLPPGYVV-NQDKS 1393
BLAST of Endoribonuclease Dicer vs. TrEMBL
Match: A0A2S1PRV8 (Putative dicerB OS=Actinia equina OX=6106 PE=2 SV=1) HSP 1 Score: 392.119 bits (1006), Expect = 2.210e-116 Identity = 394/1419 (27.77%), Postives = 652/1419 (45.95%), Query Frame = 2 Query: 26 NFSFMKTRDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIK--------DYLNVKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEH--VIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIAREFCGNIKETIINYETESFPDLVAIFKSAIEKI--ESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSC-LKFINSNIESY------ISKSDMLMCSEKCKKLINLILDLKSKNEDFKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHS---TSQYPKKLQMSVTKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSERNVTPSEDDKYDYV------PY-GENGPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNRFDNNLVIQESNDIEI-IKYEKYTATKLSTKFQNLSI-----------PANKLYLYSIKYTPSVFLKL---GINDISMFDVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFEYNLPVNSINYSLLLKCHKILFNIYSERIR-RFDK----NIFFDPSKAKSSIILTILKDNKIDFNKIKEIITNPIVDNLIKNDQSSWVGSVIRPNYITNEFGAFVISKVCHDMNADSIF---KNDMTYEKFLNKKYNIILSPNEKLSPLIKVIRLTTIQSCVNKERRFL--RNNRKLQRINESECYFVPSLTECISLSSSLWTQLCIFPTIHHRMNQIL--------------------SIEDFAHDLEFMN--------LIENSDCFEINFD---DCYNIENIES------SIYDPNVLKFINN----IFPCFMAKTCNDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNS----------------------DESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPDNELNADIEISPK--LAFQNISMDTDIPQS-----------INDLEKILNYHFKNKGYIVQAITHPSSIANR-KFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVEELLNNQ 3889 N + R YQ+E+ KA +N+++ L TG+GKTFI+ +LIK DY + K+ FFLV LV Q + L +G + G+ VD W K++W K++ ++++VMTA++F+D+L H K LS+V ++FDE HH K Y+Q+ + ++LGLTA ++ G IE ++ LE L TS D ++ E Y T D+ A K E++ E P D ND + + K K + E L LG + + F+ D+ G + + +L + S L+ + ++Y S + + S K K L+ ++ + ++ + ++F+ + L + +L + +KA F+ +++GH T+ K MS KQ ++L+ F+ +FN+LIAT+V+EEGLDV CN+VV+FD P F+SY+QSKGRARA++S YI+ + Q T A+++ + I E L+ + R I P++++ + V PY G +T+ ++ ++L +Y KL + + Y + + +L +P+ P E+I G P K++AK EACK LH+ ELD L P +N E D+E+ + + + TK + + P ++ YLY F K + S+ + LGIL+ + + + F +P G +T I + SL+ + H I SE +R F++ + P + SS T + + +IDF++ ++++ + + S+I P + E F+I ++C +++ S F TYE++ K+Y + + PLI V ++ VN FL R K + + + +F+P L S+ L + P+I HRM +L S+E DL + ++EN + D YN E ES S++ N F+ + K D +LE+ E+LGD+FLK S YLF F + D EGKLT+++ ISN L+ ++T L Y T + K ++ PP VK ES + + S + I +K++AD EA+IG+ L + G ++ +L +L + ++PD E NA + SP +F D +P S + E+ +NY F+NK Y++QA+TH S NR CYQRLEFLGD+V+DF+VT + NK+L+PG++T+LR ALVNNN FA ++V+ G+HKYL + F + V++L +++ Sbjct: 28 NQESLSARPYQVELLVKALEKNIVVCLGTGTGKTFISVLLIKELSHQIMKDYED--GGKRTFFLVNTVPLVAQQRKVIDQNTDLS--VGKYVGE---DGVDFWGKERWLKELKDHQVLVMTAQIFVDMLGHGKIQLSKVNLIVFDECHHAKKEHPYAQVMRYFTAYNRYDHPRVLGLTASIVNGKVKPHRIEQEIQTLERILRASCETSSDECVGKYATKPTEYAHPYSTRE--DVSAEEKQLQERLNQEVLFPWD-EFLND----VRDKSETMKISKSIVKECIETLEVLGASAANRVAEYFL-DVTGRFSRTRGLLSSEYSHWLMGYNSTQLRQVQKIYKTYKTELYGPSNNSVQHVSAKVKALVEILKQYGTGDK----KLCGIVFVQRRCTAAVLCEQITLLSQHDKALSFVKCEYVVGHGMVGTAASSAK-DMSCKKQDKVLEKFRNHEFNLLIATSVVEEGLDVPRCNLVVRFDFPMTFQSYVQSKGRARAKDSFYIILLPDGQDT-AELEMYQAIEEYLSHVCYDRRI--------------PTDEESTEAVDTDLLQPYRTRKGASVTMANSIALLCKYCSKLPGDRFTLINPDYKTVEVGKDAFQTTLTMPINCPIKEKIMGDPMPRKKQAKMAAAYEACKRLHQEGELDDHLFPIESLSDNE--SEDEDVEMGVAGQPKSGTKRKKQAYPRKVPDILKESLPTGPRDRCYLYG-------FTKAHNKATDSHSVEEEVLKLGILMTSELPPVRRFTLYPDE--GAVTISVIPIAKPQRLTDAQLSLIDQFHS---RISSEMLRNSFERQNLDGYYIVPLETSSS---TRMDEIRIDFDRAEKVLCQEETVRITCLEDVKSGDSIITPMHRPRE--RFIIEEICTNLSPMSAFPERSKAKTYEEYFRKEYPSLKRITRRAQPLILV---KSMPQRVN----FLVDRKKMKSKEKTKKDIHFIPELCTLEPFSAKLLLEARELPSILHRMTSLLLVMEMRKLIAEGCRVDVQQFSVEQNEEDLSTIGGDILTGNAMVENPVPQRLALSSKRDKYNNE-FESVSKNLDSLFSNNRTNFLQPDSALLLQALTTKGAGDAFDLERLEMLGDAFLKQAVSIYLFCFYTNKD-EGKLTRRKVSQISNLKLYKVSTAHHLPGYIQTTVLDRK-SWAPPCLQVKPQSTGKILQERFTSKKKAKKQKLDSESKQEDFSSQ--TISDKSVADSIEALIGAYLVSGGYKGALYFLDYLGIKVLPD-EDNAALSGSPSNYASFLTPPCDP-LPSSRPEDLENAALELQGFERRINYEFRNKIYLLQALTHASYAHNRLTPDCYQRLEFLGDAVLDFVVTECLYSRNKRLSPGELTDLRQALVNNNIFAKIAVEHGYHKYLKQTSPEWFKKIGDFVQKLEDDE 1378 HSP 2 Score: 60.077 bits (144), Expect = 2.210e-116 Identity = 26/54 (48.15%), Postives = 36/54 (66.67%), Query Frame = 3 Query: 3939 KVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHE 4100 KVLGDIFES+AGA+F+DS D+ W VY M+ + + N+P N I ++HE Sbjct: 1405 KVLGDIFESVAGAVFLDSGMDVVVTWKVYYRMMKPYIDYYSKNVPYNPIRLVHE 1458
BLAST of Endoribonuclease Dicer vs. TrEMBL
Match: A0A131YTU7 (Endoribonuclease Dicer OS=Rhipicephalus appendiculatus OX=34631 PE=3 SV=1) HSP 1 Score: 402.134 bits (1032), Expect = 1.065e-112 Identity = 395/1462 (27.02%), Postives = 664/1462 (45.42%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDYLNV------KSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISE--HVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVIT--SMDNIAREFCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDLH---GAVNNDSMFFDEKTKL-YLDEFLS-CLKFINSNIESYISKSD---MLMCSEKCKKLINLILDLKSKNEDFKN-----------TISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQY--PKKLQMSVTKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSERNVTPSEDDKY-DYVPYGENG-PRITLNSASSVLNRYLGKLRCNSISEPKIIY---TYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNRFDNNLVIQESNDIEIIKYEKYTATKLSTKFQN-------LSIPANKLYLYSIKYTPSVFLKLGINDISMF------------DVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFEYNLPVNSINYSLLLKC-HKILFNIYSERIRRFDKNIF-FDPSKAKSSIILT-ILKDNKIDFNKIKEIITNPIVDNLI-------------KNDQSSWVGSVIRPNYITNEF--GAFVISKVCHDMNADSIFKNDMTYEKFLNKKYNIILSPNEKLSPLIKVIRLTTIQSCVNKERRFLRNNRKLQR--INESECYFVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDFAHDL------------------EFMNLIENSDCF-------EINFDDCY----NIENI------ESSIYDPNVLKFINNIFPCFM-----AKTCNDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESV------------------------EVNNS--------------------------REFILIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVP---DNELNADIEISPKLAFQNI-SMDTDIPQSIND------------------LEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE 3871 R+YQ+E++ A EN I+ L TG+GKTFIA MLIK+Y + K+ FFLV LV Q ++K L+ +G + G M VDNW K++W K+ + +++VMT VF I++H +S+V LI DE H K Y ++ V+ ++LGLTA +I +E V LE L+ +V+T + ++AR + E ++ +++ P + I + +F P E + + + + L VL +G+ L ++ +E+ +N++S+ + + L +LDE C + I IES ++SD + M + K K+L+ ++ + + E +N ++ +IF+ E+I L L L + +I + FI+GH + K+ MS KQ +L+ F+ ++ N+L+AT+V+EEG+DV C++VV+FD P DFRSY+QSKGRARAQ S Y++ T +A N ++ + IL ++ E + SE ++ + D+ Y+P E+G PRITL+SA ++NRY L + + K Y T ++ + ++ LPM +P E I+G P K+ AK LE CK LH+ +E D+ LLP R L + + +D E + A K TK + + A L + Y + +N S D T+ +LL + FPI+ G V + L S L+C H+ I+ + +R ++ + FD S A S +L ++KD + +I P VD ++ K + + G+V+ P Y + F + ++ D+ DSI ++ +L+ +++ ++ + P+++V+ + + R + ++ L++ N VP + +S+S+W + P+I +R+NQ+L +E+ + +F + + S E+ F + ++E I S Y P LK P + + D +LE+ E+LGDSFLK+ + LF S EG LTQ RS++I N+NL L ++ + F P N+LPP ++V E + E+ NS R+ + +K++AD EAIIG+ L G +++ ++W+ L L D + + D E + AF + T + + I+D EK L Y F+++ +++QA TH S NR CYQRLEFLGD+VID+LVT Y+ K NPG++T+LRS+LVNN FA L V+ G H+ L + + +F+ + + V+ Sbjct: 35 REYQVELFEAAVKENTIVCLGTGTGKTFIAVMLIKEYEASIRIPFEEGGKRTFFLVPTVPLVVQQQKAIKCQTDLR--VGGYVGDMN---VDNWNKERWEKEFVESQVLVMTPDVFKVIIHHGFLKMSRVNLLILDECHRAVKQHTYREVMRCMDVLEPHERPRVLGLTASVINSKATEHQVERKVHDLEMALHSRVLTVSNQQSMAR-YGTKPVEVLVEHQSTGSPSICGI---EVPTFPTFRP---------------ETSQGQEMDRRLRSLTKVLQDMGVWCGHLAVEVIMEEARRMLDGLNDNSVLREVEVCLEWLDEVRQRCKRHI---IESNAAQSDEPLLAMATPKLKRLLQILWAFRPR-ERLQNGNDADAAEPPMSLCGIIFVKERIVARILRDWLRELANKIPRYAYIMAEFIVGHGANLQLGSKEAGMSFKKQCRVLEQFRRQECNLLVATSVVEEGMDVPKCSLVVRFDFPDDFRSYVQSKGRARAQRSLYVMMTSRDDYNKAAANLRNYLS--IEAILMNKCHERKAPL-TSEVVLSFAVDELLPPYMPVREDGAPRITLSSAIGLVNRYCMILPSDMFTHLKPTYQLSTVTVEDKEMYVCTIYLPMTSPLKEAIRGRPMETKKYAKMAAALETCKRLHQINEFDNCLLPVKRM---LKLLDDSDNEETEEVPPGAPKPGTKKRRRVYQKRVCRLFAEARVLEAGHYRLHILTTRLVNMASELQNWRNETLVDPEDCPTWFALLLREDM---PCLPSFPIFTRSGDEEVTV-VRAGLVSLSAGECCRLQCFHRA---IWDDVLRMKERPLLQFDLSSAPSCPVLAPVIKDGDVS------MIDWPFVDMVVNGINGKHATGTAYKFNPCDYEGAVVVPRYAPPHYHHSTFYVKEIRTDLTVDSI-DGKGSFRDYLSLIHHMHVT--DSTQPILEVVFTEVRLNLLTPRYRNRKGDKFLKKTATNRRAELRVPEFCDVHPVSASIWRKAVCLPSIVYRLNQLLVMEELRASIAAATGIGVKTFRGRWPSVDFQSAVSGSATISFSPAPNEVEFGNLLRGHGDVEEIRMPRLSRSFEYQPE-LKGNPGPSPGLLLEAVTSAKAADGFDLERLEVLGDSFLKYAVTIDLFCGSTSA-QEGLLTQTRSRIICNRNLHQLGSKMHVGSMITQEQFEPLRNWLPPGYLVPEGAEDIMMDVDFVFWVIREDTKILDKLNYKELCNSYEHYKDEMKRPEFVPPPRNTSGPVPIRQSSQLSDKSVADSVEAIIGAFLLVTGPIGALRVMKWMGLKLQQCNLDAQGSGDTESTAVPAFMGFPAPRTSLLRYIDDPEGHLVSPSSPWHAALCLAEKTLGYTFRDRSFLLQACTHASYYKNRLTDCYQRLEFLGDAVIDYLVTRYLYEGPHKFNPGQLTDLRSSLVNNTFFAALVVRHGLHRCLLHCNPGLFNAVARFVQ 1444
BLAST of Endoribonuclease Dicer vs. TrEMBL
Match: H6WZT1 (Dicer-2 OS=Penaeus japonicus OX=27405 GN=Dcr2 PE=2 SV=1) HSP 1 Score: 391.349 bits (1004), Expect = 2.843e-112 Identity = 388/1398 (27.75%), Postives = 659/1398 (47.14%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDYLNV------KSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIK---------ILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMD-NIAREFCGNIKETIINYETESF-PDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNI-ESYISKSDMLMCSEKCKKLIN----LILDLKSKNEDFKNT---ISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIG-HSTSQYPKKL-QMSVTKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSERNVTPSE-------DDKY--DYVPYGENGPRITLNSASSVLNRYLGKLRCNSISE--PKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNRFDNNLV-----IQESNDIEIIKYEKYTATKLSTKFQNLSIP-----ANKLYLYSIKYTPSVFLKLGINDISMFDVST---YLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFEYNLPVNSINYSL---LLKCHKILFNIYSERIRRFDKNIF-FDPSKAKSSIILTILKDNK-IDFNKIKEIIT-NPIVDNLIKND-------QSSWVGSVIRPNYITNEFGAFVISKVCHDMNADSIFKNDM----TYEKFLNKKYNIILSPNEKLSPLIKVIRLTTIQSCVNKERRFLRNNRKLQRINESECY--FVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDFAHDLEFMNLIENSDCFEINFDDCYNIENIESSIYDPNVLKFINN-----IFP-----CFMAKTCNDRT--NLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNS-DESV------------------EVNNSREFIL---------------------------IKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPDNELNADIE-ISPKLAFQNISMDTDI---PQSINDLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKM 3844 R YQ+E++N A +N I+ L TGSGKTFI+ +LIK+ + K+ F+V LVHQ +++ + E+G + G M VD WT + W ++ K++++V+ A++FLD++ H K LS V LI DE HH T S +I +R ++K I+GLTAC++ +++ ++ LE ++C ++TS+D ++ KE +I + TES D K+ + +E I D +N + K +KK + ++S LG IE L + M D K + LK ++ + E Y++ C K K + N + +KS E F + LIF+ + L LL + K N F F+ ++++G +S +L ++ + KQ E L+ F+ + N LI+T+V+EEG+D+ CN+VV+FD PQ++R+Y+QS+GRARA+ S Y++ E + ++E L I + IED L RN+ ++ +D+Y Y PYG GP+IT NSA ++N Y GK + ++ P+++ + V + LP+ AP + I G +NK AK+ L+ C LH+ ELDS+L P D++LV + + E T + ++L P LYSI P F + G + D ST +G + + + FP++ V + P+ ++ SL + K H+++F E I ++N+ FDP K+K + + L ++ ID N + EI + + I +IK+ +S + ++I P Y + +S + ++N + F + +YE++ ++Y + L +K PL++ + KE +L+ K R + FVP I + +SLW Q+ P+I HR+N + +F + + ++ + ++ + E + SS + V +F++ I P F K ++ NLE+ E+LGDSFLKF+T + LF +++ +EG+L+Q R + + NK LF LA QKGL+ NPK N P F +K +ES+ +V + +E + + +K++ADC EAIIG+ L G + + +L WL + + DN L + + + + + D I ++ LEK + Y F+++ +IVQA+TH S + N+ CYQRLEFLGD+V+D+LVT +I +PG++++LRS V N+T + K H++L + + K+ Sbjct: 15 RSYQLELFNCALKKNSILVLGTGSGKTFISILLIKELGHQIRESLKGGSKRTVFVVNTVPLVHQQAAAIQQHTPF--EVGKYEGSM---GVDYWTDEHWQNELEKHEVLVVVAQIFLDLILHAKLPLSSVNLLIMDECHHATGSHPMREI----MRAYENLKQHSPEKLPRIMGLTACVVHRKCKRREVLKYMESLEAAMDCALVTSVDPETVLKYTTGPKEKVICFYTESITSDYQETVKAQLAGLECDIITDCF--------INYKAR--KQIKKKVKNIEHIMSDLGDWCVARAIKYEIEHLD---ETEGMEEDPKIR-------EILKLLSEKLREVYVA------CELKEKGMANPVEHITFKVKSLLEIFSKCNKEVFGLIFVESRNTAKILYDLLLEIAKQNPEFSFVKPSYVVGCNSRPGIDIRLAELELRKQRETLENFRRGETNFLISTSVLEEGVDIRKCNVVVRFDMPQNYRAYVQSRGRARAKPSVYLLMVEEAK-----------VSEMLECIGVYKEIEDSLQYLCHGRNLPTAQTTRKHFAEDEYIKPYEPYGPVGPKITSNSAIQLVNLYCGKFPSDKFTDNLPEVVEEVKE---GMTTVGIRLPIMAPLKDMIYGSGWANKDLAKKSAALQLCIKLHEMGELDSNLRPKESNDDSLVEGLVDLPPEEPVPEGTSESGTRKRRRVHAKDLCYPFSNEHGGSYCLYSIDIKP--FGQSG--ESVTIDSSTCISKIGFICKEGLVSS----PFPLFF-SKWGDVMVNVREVGPLTDLDTSLFDKIKKFHRLVF----ENIIELNENLMSFDPEKSKWDVFIVPLDFSEIIDLNMLNEIYSFDSIKPVMIKSHNQNTKFVRSKFEDALIYPQYGIPSNQMYYVSHIFDELNPCNTFPEGLKGYESYERYFLERYGLEL--KDKNQPLLEAKH-------IPKELNYLKKIPKKAREKKGNLVPRFVPEFCGLIPIKASLWWQIMCMPSIFHRLNSFNLMHEFIVKTKITSPAIKNEVLDFSWTKKFIYELLGSS--ENTVPRFLDRKKQEYIHPFSMLHAFTLKEASEDKEFNLERLELLGDSFLKFVTGEALF-LRDESYHEGRLSQSRGKRVCNKTLFRLACQKGLEGKIAAKVLNPKKNGSIPGFRIKPEFEESLRSLEVPHDFWHQVPNTVKDVGDLKEAVFALMSDDASKDEKAEKKGSCYNPWTEHEVSDKSIADCVEAIIGAYLRVCGNEAAAAFLHWLGVGVFRDNSLLMEPKTVRTARVRKECAQDLTIFYKKAVLDRLEKKIGYTFRDRSFIVQAVTHSSYVNNQVTDCYQRLEFLGDAVLDYLVTCHIFCRFTDYSPGQLSDLRSYFVKNDTLGRAAAKGKLHQHLLHINPKL 1338 HSP 2 Score: 47.3654 bits (111), Expect = 2.843e-112 Identity = 24/62 (38.71%), Postives = 34/62 (54.84%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQMK 4118 + K LGD+ E+I GA+++DSN LK W V + +E+ L I N I L E+ P K Sbjct: 1372 IPKALGDMVEAIIGAVYLDSNRSLKVAWQVIERILEDLLNTSIQTIQLNPIRQLLEEVPGEK 1433
BLAST of Endoribonuclease Dicer vs. TrEMBL
Match: R9R7R6 (Dicer 2 OS=Penaeus monodon OX=6687 PE=2 SV=1) HSP 1 Score: 394.815 bits (1013), Expect = 3.740e-112 Identity = 388/1394 (27.83%), Postives = 659/1394 (47.27%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDYLNV------KSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQIS---EHVIRTTSD--IKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIA-REFCGNIKETIINYETESF-PDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLK------FINSNIESYISKSDMLMCSEKCKKLINLILDLKSKNEDFKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIG-HSTSQYPKKL-QMSVTKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSERNVTPS-------EDDKY--DYVPYGENGPRITLNSASSVLNRYLGKLRCNSISE--PKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNRFDNNL------------VIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFLKLGINDISM----FDVSTYLGI-----LLPNPIHQNKDFMKFPIYLVGGMTSVEIKFEYNLPVNSINYSLLLKCHKILFNIYSERIRRFDKNIFFDPSKAKSSIILTILKDNK-IDFNKIKEIIT----NPIV----DNLIKNDQSSWVGSVIRPNYITNEFGAFVISKVCHDMNADSIF----KNDMTYEKFLNKKYNIILSPNEKLSPLIKVIRLTTIQSCVNKERRFLRNNRKLQRINESECY--FVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDFAHDLEFMNLIENSDCFEINFDDCYNIENIESS------IYDPNVLKFIN--NIFPCFMAKTCNDRT--NLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNS-DESVE------------------VNNSREFIL---------------------------IKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPDNELNADIE-ISPKLAFQNISMDTDI---PQSINDLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKM 3844 R YQ+E++N A +N I+ L TGSGKTFI+ +LIK+ + K+ F+V LVHQ +++ + E+G + G M VD WT + W ++ K++++V+ A++FLD++ H + LS V LI DE HH T S +I E + + + D +I+GLTAC++ KD+ ++ LE ++C ++TS+D + ++ KE +I Y TE+ D K+ + +E I D + K +KK + ++S LG IE L + M D K + E L LK +I ++ + + + K K+L+ + K E F LIF+ + L LL + + N F F+ ++++G +S +L ++ + KQ E L+ F++++ N LI+T+V+EEG+D+ CN+VV+FD PQ++R+Y+QS+GRARA+ S Y++ E + ++E L I R IE L +RN+ + E+D+Y Y PYG GP+IT NSA ++N Y GKL + ++ P+++ + V + LP+ AP + I G +NK AK+ L+ C LH+ ELDS+L P D++L V + + + K ++ A +L F N + LYSI P G D S+ F +G LL +P FP++ V + + P+ ++ SL K K ++ I + I FDP ++K + + L ++ ID + + EI + P++ + K +S + +I P Y + +S++ ++N + F K +YE++ ++Y L +K PL++ + KE +L+ K + + FVP I + +SLW Q+ P+I HR+N + + +F + + + + + ++ + E + SS D ++I+ N+ F K ++ NLE+ E+LGDSFLKF+T + LF +++ +EG+L+Q R +L+ NK LF LA Q+GL+ FNPK N P F+VK +ES+ V + +E + + +K++ADC EAIIG+ L G + + +L WL + + DN L + + I + + D +I ++ LEK + Y F+++ +IVQA+TH S + N+ CYQRLEFLGD+V+D+LVT +I +PG++++LRS V N+T + K H++L + + K+ Sbjct: 15 RSYQLELFNCALKKNSILVLGTGSGKTFISILLIKELGHQIRESLKGGSKRTVFVVNTVPLVHQQAAAIQQHTPF--EVGKYEGSM---GVDYWTDEHWQNELEKHEVLVVVAQIFLDLILHARLPLSSVNLLIMDECHHTTGSHPMREIMRAYETLKQHSPDKLPRIMGLTACVVHRKCKRKDVLKYMESLEAAMDCALVTSIDPVTVLKYTTGPKEKLICYFTENITSDYQETVKAQLAGLECDIITDCF----------INEKARKQIKKKVKNIEHIMSDLGDWCVARAIKYEIEHLD---ETEGMEEDPKVR----EILKLLKERLRDVYIACELKEKCMSNPLEHITFKVKRLLEIFS--KCNKEVFG-----LIFVERRNTAKILYDLLLEIAQQNPVFSFVRPSYVVGCNSRPGIDIRLAELELRKQKETLEKFRKEETNFLISTSVLEEGVDIRKCNVVVRFDIPQNYRAYVQSRGRARAKPSVYLLMVEEAK-----------VSEMLECIGVYREIEASLQYLCHDRNLPTAQVTRKHFEEDEYIKPYEPYGPVGPKITSNSAIQLVNLYCGKLPSDKFTDNLPEVVEEVKE---GIVTVGIRLPIMAPLKDMIYGSGWANKDLAKKSAALQLCIKLHEMGELDSNLRPKESSDDSLVEGLVDLPPEEPVPEGTPESGTRKRKRIYAKELCYPFSN--VHRRSYCLYSINIKP-----FGQTDQSVTIDSFTCINKIGFICKEGLLCSP---------FPLFF-SKWGDVMVNVKEVGPLTDLDTSLFDKIKKFHRLVFENLIELKENLINFDPEESKWDVFVVPLDFSEIIDLDLLNEIYSLDSIKPVMMRSHNQSTKFVRSKFEDGLIYPLYGIPSDQMYYVSQIFDELNPCNTFPEGKKGYESYERYFLERYG--LEIKDKNQPLLEAKH-------IPKELNYLKRIPKKAKEKKGNLVPRFVPEFCGIIPIRASLWWQIMCMPSIFHRLNSLNLMHEFIMETKITSPAVKNKVLDFSWTRKFIHELLGSSENAVPIFLDRKKQEYIHPFNMLHAFTLKEASEDKDFNLERLELLGDSFLKFVTGEALF-LRDESYHEGRLSQSRGKLVCNKTLFRLACQRGLEGKIAAKVFNPKRNGHIPGFIVKAEFEESLRSHEVPHDIWHQVPNTVKNVGDLKEAVYDLMSDDASKDDKAEKKGSCYNPWTEHEVSDKSIADCVEAIIGAYLLVCGNEAATSFLDWLGVGVFKDNSLLMEPKTIRTAYVCKEHAQDLNIFYKKAVLDRLEKKIGYTFRDRSFIVQAVTHSSYVNNQVTDCYQRLEFLGDAVLDYLVTCHIFCRFTDYSPGQLSDLRSYFVKNDTLGLAAAKGKLHQHLLHLNPKL 1338 HSP 2 Score: 43.1282 bits (100), Expect = 3.740e-112 Identity = 22/62 (35.48%), Postives = 33/62 (53.23%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQMK 4118 + K LGD+ E+I GA+++DSN LK W V + + + L I N + L E+ P K Sbjct: 1372 IPKALGDMVEAIIGAVYLDSNRSLKVAWQVIERILGDLLNTPIQAIQLNPVRQLLEEVPGEK 1433
BLAST of Endoribonuclease Dicer vs. TrEMBL
Match: U3MHS9 (Dicer 1 OS=Nematostella vectensis OX=45351 PE=2 SV=1) HSP 1 Score: 371.318 bits (952), Expect = 2.470e-110 Identity = 370/1408 (26.28%), Postives = 638/1408 (45.31%), Query Frame = 2 Query: 38 MKTRDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDY-LNVK-----SDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIAREFCGNIKETIINY---ETESFPDLVAIF-KSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDL---HGAV--NNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKSDMLMCSEKCKKLINLILDLKSKNEDFKN-TISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQY--PKKLQMSVTKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHETQKTEA--QIQKFNLINEELNKILKSRVIEDELNKDDSERNVTPSEDDKYDYVPYGEN-GPRITLNSASSVLNRYLGKLRCNSISE--PKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNRF-DNNLVIQESND--------IEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFLKLGINDISMFDVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFEYNLPVNSI-----------NYSLLLKCHKILFNIYSERIRRFDKNIFFDPSKAKSSIILTILKDNKIDFNKIK-EIITNPIVDNLIKNDQSSWVGSVIRPNYITNEFGAFVISKVCHDMNADSIFKND---MTYEKFLNKKYNIILSPNEKLSPLIKVIRLTTIQSCVNKER-RFLRNNRKLQRINESE-CYFVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDFAHDLEFMNLIENSDCFEINFDDCYNIENIESSIYD------------PNVLKF------INNIFPCFMAKTCNDRT-----------------------NLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNF-------------------------VVKNSDESVEVN------NSREFIL---IKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPDNELNADIEISPKLAFQ-------NISMDTDIPQ-------SINDLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMF 3847 + R YQ E+ A +N+I+ L TG+GKTFI+ MLIK+ V+ K+ FFLV L Q + LK + F G M VD W+ ++W K++ +++VMTA++FLD+L H + LS V ++FDE HH K Y QI + + +ILGLTA +I K +E + +LE + TS D +F +E I Y +TE DL + ++ + + F+ +D S ++ K +K +S+ +L +G + + F++DL G V N+D ++ + L F + +++ + D+ K K L+ ++ + + ++ ++F+ + L L I+ + + +FI +F++GH S QM TKQ ++LK+F++ + N+L+AT+V+EEGLDV CN VV+FD PQ+FRSY+QSKGRARA+NS +IV + A +++++ L + + R + + + +D+Y PY + G R+T ++ S L Y KL + + P+II + F +L+LP+ P + I+G+ K ++Q EACK LH+ ELD +L P + + ES D + Y ++L + YSI + + ++++ +GI+L P+ +F ++L G V IK + P S+ + LL + ++ + ++++ + + D A I L ++D + KI I +D+L D ++I P + + +V+ +VC ++N S F + TY ++ K Y+ ++ + PL++V ++ ER FL N +K R + +F+P L ++ +SL + I P++ HRM L + ++ + + + C N I + +S+ P V + I A N+RT +LE+ E+LGD+FLK S +L+ D D EGKLT++++ +SN+ L+ +A + GL +Y T + + ++ P+ + + E++ +R+ L I +K++AD EA+IG+ LT G ++ +L WL + ++P++ ++ D P+ + + I+ PQ + E + Y F NK Y++QA+TH S NR CYQRLEFLGD+++DFLVT I N L+PG++T+LR ALVNNN FA ++VK GF+K L + F Sbjct: 23 LTPRPYQSELVRIALEKNIIVCLGTGTGKTFISVMLIKELSFQVRKSRKEGGKRTFFLVNTVPLAKQQKEVIAKNTDLK--VKEFIGDM---GVDFWSDEQWKKELDDNQVLVMTAQIFLDLLGHARLRLSDVNLIVFDECHHARKGHPYKQIMGY-FEESDYPRILGLTASII-NTKETHKMEQLICELEATMRAACQTSDDITVSQFATRPEEIIYPYSNDKTEVEQDLNQLLVETVLNPWQEFV-------DDLRAS--GQKEVLKGVKSLISDCRQILDEMGASAANRVAEYFLKDLERVQGLVVANDDVIWLYQYCATQLRRFRE-IYSVHTIAHEVSEEDDLTHLLPKAKALVEILKQYGNPQASTPDISLCGIVFVERRYTAKILCEQLNIIATRDPSLEFIKCDFVVGHGASGINSSSDTQMGSTKQEKVLKMFRKHQINLLVATSVVEEGLDVPKCNFVVRFDFPQNFRSYVQSKGRARAKNSAFIVLVEDKDSEWAGLEVERYQETENYLQEKCQGRPLP-------TSEECEQANEDQY-LQPYVTSLGARLTQRNSLSFLLSYCSKLPGDQYTRLMPEIITEEVEGAF---RSTLMLPINCPLRDTIEGIVMPRKMLSRQAAAYEACKRLHQMGELDDNLRPVVSLSEESDEGTESEDDGKSKGKTGTKKRRRCYNRKIPDVLKESLPTAGAAYWFYSIDIKSPETRRAPVELGTLWN----MGIMLRKPLPA---IRRFQLFLDSGPVIVSIKPCDSTPTPSVPGFHLRIIEAFSRCLLSRALRMPLEVTADKLHGY-HVVLLD--SACKGIDLARMRDVERQMEKIDLPIRDQDALDSLASGD------TIITPKHREKQ-QRYVLERVCPELNPTSPFPDRSKARTYAEYYEKTYDCHIT--DMHQPLLQVRNMS--------ERINFLVNRKKQSRKKSNHVVHFIPELCSHDAIPASLLSNAQILPSVLHRMTSFLLAVELREKIKSKSDVIDEACAPEN--TLSVIGGVPASVIGRSLSPLRKKRSPPRVRSYDLFDKEFQEISQSLQAMFSNERTVLLHPDSELLLQALTTKSAGDAFDLERLEMLGDAFLKQAVSIFLYCCYKDGD-EGKLTKRKTSQLSNRALYRVACRLGLPEYLQNTQLD-RVSWTTPSLQAAEVEPVGSPGKSQKTASNKRCQSASRQPENRPEMSTLEKPETARDKALHQSIPDKSVADSVEALIGAYLTTCGYQGALHFLDWLGVKVLPESPMSTD---GPRASSKYSIFTRPTITEYNSNPQVLKRFAVQLQGFENRIGYEFSNKLYLIQALTHASYSQNRLTDCYQRLEFLGDALLDFLVTELIYSRNTNLDPGELTDLRQALVNNNIFAEIAVKHGFNKSLFQMSPEWF 1368 HSP 2 Score: 60.8474 bits (146), Expect = 2.470e-110 Identity = 31/83 (37.35%), Postives = 46/83 (55.42%), Query Frame = 3 Query: 3939 KVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQMKCTFENQEFSNLIKLTAILPNGSV 4187 KVLGDIFES+AGAIF+DS DL W VY ++ + + ++P+N + ++HEK + L +LP+G V Sbjct: 1408 KVLGDIFESVAGAIFLDSGMDLVLTWRVYYRMLKPYIDAYSADVPKNPVRLIHEK-----------DADALFSEAKVLPSGKV 1479
BLAST of Endoribonuclease Dicer vs. Ensembl Cavefish
Match: dicer1 (dicer 1, ribonuclease III [Source:NCBI gene;Acc:103033221]) HSP 1 Score: 226.868 bits (577), Expect = 2.562e-59 Identity = 269/1058 (25.43%), Postives = 480/1058 (45.37%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKD---YLNVKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIARE-FCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTL-KKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKS--DMLMCSEKCKKLINLILDLKS--------------KNED------------------------------FKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQ-YPKKLQMSV--TKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHE--TQKTEAQIQKFNLINEEL-NKILKSRVIED-ELNKDDSERNVTPSEDDKYDYVPYGENGPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKG--LPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNR--------------FDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFL--KLGINDISMF---DVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFE-YNLPVNSINYSLLLKCHKILFNIYSERIRRFDKNIF-FDPSKAKSS---IILTILKDNK---IDFNKIKEIITNPIVDNLIKNDQ-----------SSWVGSVIRPNYIT-NEFGAFVISKVCHDMNADSIFKND--MTYEKFLNKKYNIILSPNEKLSPLIKV------IRLTTIQSCVNKERRFLRNNRKLQRINESECY-----FVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDF 2884 R YQ+E+ A N I+ LNTGSGKTFIA +LIK+ + + ++ FLV +S V Q ++++ + L+ G+ +S +W ++KW + I + +++VMT +FL L LS + L+FDE H Y I + +ILGLTA ++ G D+E ++ LE L T+ D + + + +E +++ + D + ++ + +++ + H ND + S++ E + + K+ L++ +VL+ LG + + +L + ++ + K L+ D + L+ +++ E + S + D+ + K KL+ ++ + K +N+D F N + +IF+ + + LN L+ K + +I+SNFI GHS + P+ QM V KQ E+L+ F+ + N+LIAT+++EEG+D+ CN+VV+FD P ++RSY+QSKGRARA S YI+ T+ E ++ + I + L NK KS D E++ + N+ P + D + GPR+T+N+A +NRY +L + + + + + + +L LP+ +P E IKG +PC+ R A++ V L C+ LHK ELD L+P + + N+ + + Y K L + +P YLY I + L +L ++ D + GIL PI + FP+Y G ++ I+ + +++ L+ + H+ +F+ I R +K F P++A S+ + L I+ D+ +DF K E I I N Q + +VI P Y ++ F ++ V D+ S F + T+ ++ KYN+ LS + PL+ V + L T + +N++ + L + +R + E VP L + +SLW + P+I +R++ +L+ E+ Sbjct: 35 RKYQVELLEAALEHNTIVCLNTGSGKTFIAVLLIKELSHQIRRGNGRRTVFLVNAASSVVQQASTVRTHSDLQ------VGEYTDS-CTSWLEEKWRQDIMENQVLVMTCHIFLHALRSGVLCLSTINLLVFDECHLAITDHPYRDIMKKCEDYRGCPRILGLTASILNGKCDPSDLEEKIQNLERILKSNAETATDLVVLDRYASQPREVVLD--CGPYQDKSGLSETLLNELDEAL----HFLNDCNLSVHREDRDPTYISKQVLNDCRAVLTVLGPWCADKAAGIMVRELQKYIKHEQEELNRKFLLFTD---TILRKLHALCEEHFSPASLDLKFVTPKVIKLLEILHEYKPFERQQFESVEWYNNRNQDNYVSWSDSEDDDDDEEVEVKEKPEANFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHSIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPVSNYIMLADSECTKAFEEDLKTYKAIEKILRNKCSKSAECNDYEVDSVLDDDNILPPYVLRTD-----DGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCKTAELKASVYQSTLFLPINSPLREPIKGPLMPCA--RLAEKAVALHCCEKLHKIGELDDHLMPVGKETVKYEEELDLHEEEETNVPGRPGSTKRRQCYPKAIPECLRGSY---PVPEQSYYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPR---IPHFPVYTRSGEVTISIELQKSGFSLSAAQLELITRLHQYIFS----HILRLEKPALEFRPTEADSAYCVLPLNIVGDSSTLDMDF-KFMEDIEKSEARIGIPNTQYTKQNPFTFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLSNVNQ--PLLDVDHTSSRLNLLTPRH-LNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEEL 1055 HSP 2 Score: 130.183 bits (326), Expect = 2.400e-41 Identity = 75/205 (36.59%), Postives = 120/205 (58.54%), Query Frame = 2 Query: 3344 IKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPDN------------ELNAD-----IEISPKLAFQNISMDTDIPQSIN---DLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE-ELLNNQLQ 3895 I +K++ADC EA++G LT+ G + +L L L ++P E AD ++I P+ F + + + I+ + EK +NY FKNK Y++QA TH S N CYQRLEFLGD+++D+L+T ++ + ++ +PG +T+LRSALVNN FA L+VK+ +HKY ++FH++ V+ +L N++Q Sbjct: 1527 IADKSIADCVEALLGCYLTSCGERAAQMFLCSLGLKVLPVEKRSRKKVEEAACEGPADPQYGWLKIPPRCMFDHPDAERTLNHLISGFENFEKKINYTFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQ 1731 HSP 3 Score: 60.8474 bits (146), Expect = 2.400e-41 Identity = 28/60 (46.67%), Postives = 39/60 (65.00%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQ 4112 V K +GDIFES+AGAI++DS L+ VW VY M ++KF N+PR+ + L E P+ Sbjct: 1752 VPKAMGDIFESLAGAIYMDSKMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPE 1811 HSP 4 Score: 73.9442 bits (180), Expect = 1.372e-12 Identity = 39/96 (40.62%), Postives = 57/96 (59.38%), Query Frame = 2 Query: 3047 NDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSRE 3334 +D NLE+ E+LGDSFLK + YLF D +EG+L+ RS+ +SN NL+ L +KGL + F+P N+LPP +VV ++ +S E Sbjct: 1276 SDGFNLERLEMLGDSFLKHAITTYLFCTYPDA-HEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKRGMDKWDSDE 1370
BLAST of Endoribonuclease Dicer vs. Ensembl Cavefish
Match: drosha (drosha ribonuclease III [Source:NCBI gene;Acc:103043153]) HSP 1 Score: 82.4185 bits (202), Expect = 3.436e-15 Identity = 66/265 (24.91%), Postives = 126/265 (47.55%), Query Frame = 2 Query: 3065 EQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGID----------YSIQYLQ--WLNLPLVPDNELNADIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANR-KFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKY 3820 E+ E LGD+ ++F+TS +L++ + EG L R+ ++ N++L LA + LD++ Y + P+ + + SD +A+C EA+IG++ G+ ++ + L+ WLN P P +++ L + + + + Q + D E+ + F + + +A T + N QR+EFLGDS++ + T Y+ ++ + G +T LRS+LVNN T A ++ + G ++ Sbjct: 907 ERLEFLGDAVVEFLTSVHLYYLFPSLE-EGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHG--------PD-LCRESD--------------LRHAMANCFEALIGAVYLEGGLQEAKLLFGRLLFNTEDLREVWLNYPPHP-------LQVQEPLTDRQLIETSPVLQKLTDFEEAIGVLFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEF 1140
BLAST of Endoribonuclease Dicer vs. Ensembl Cavefish
Match: drosha (drosha ribonuclease III [Source:NCBI gene;Acc:103043153]) HSP 1 Score: 82.4185 bits (202), Expect = 3.524e-15 Identity = 67/265 (25.28%), Postives = 125/265 (47.17%), Query Frame = 2 Query: 3065 EQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGID----------YSIQYLQ--WLNLPLVPDNELNADIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANR-KFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKY 3820 E+ E LGD+ ++F+TS +L++ + EG L R+ ++ N++L LA + LD++ Y + P+ RE L +A+C EA+IG++ G+ ++ + L+ WLN P P +++ L + + + + Q + D E+ + F + + +A T + N QR+EFLGDS++ + T Y+ ++ + G +T LRS+LVNN T A ++ + G ++ Sbjct: 878 ERLEFLGDAVVEFLTSVHLYYLFPSLE-EGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHG--------PDLC-------------RESDL--RHAMANCFEALIGAVYLEGGLQEAKLLFGRLLFNTEDLREVWLNYPPHP-------LQVQEPLTDRQLIETSPVLQKLTDFEEAIGVLFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEF 1111
BLAST of Endoribonuclease Dicer vs. Ensembl Cavefish
Match: drosha (drosha ribonuclease III [Source:NCBI gene;Acc:103043153]) HSP 1 Score: 82.0333 bits (201), Expect = 3.836e-15 Identity = 67/265 (25.28%), Postives = 125/265 (47.17%), Query Frame = 2 Query: 3065 EQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGID----------YSIQYLQ--WLNLPLVPDNELNADIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANR-KFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKY 3820 E+ E LGD+ ++F+TS +L++ + EG L R+ ++ N++L LA + LD++ Y + P+ RE L +A+C EA+IG++ G+ ++ + L+ WLN P P +++ L + + + + Q + D E+ + F + + +A T + N QR+EFLGDS++ + T Y+ ++ + G +T LRS+LVNN T A ++ + G ++ Sbjct: 907 ERLEFLGDAVVEFLTSVHLYYLFPSLE-EGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHG--------PDLC-------------RESDL--RHAMANCFEALIGAVYLEGGLQEAKLLFGRLLFNTEDLREVWLNYPPHP-------LQVQEPLTDRQLIETSPVLQKLTDFEEAIGVLFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEF 1140
BLAST of Endoribonuclease Dicer vs. Ensembl Cavefish
Match: drosha (drosha ribonuclease III [Source:NCBI gene;Acc:103043153]) HSP 1 Score: 77.411 bits (189), Expect = 1.116e-13 Identity = 62/253 (24.51%), Postives = 118/253 (46.64%), Query Frame = 2 Query: 3065 EQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPDNELNADIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANR-KFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKY 3820 E+ E LGD+ ++F+TS +L++ + EG L R+ ++ N++L LA + LD++ Y + P+ + + SD +A+C EA+IG++ G+ + L + E+ L + + + + Q + D E+ + F + + +A T + N QR+EFLGDS++ + T Y+ ++ + G +T LRS+LVNN T A ++ + G ++ Sbjct: 907 ERLEFLGDAVVEFLTSVHLYYLFPSLE-EGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHG--------PD-LCRESD--------------LRHAMANCFEALIGAVYLEGGLQEAKLLF----------GRLLFNTEVQEPLTDRQLIETSPVLQKLTDFEEAIGVLFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEF 1125
BLAST of Endoribonuclease Dicer vs. Ensembl Sea Lamprey
Match: dicer1 (dicer 1, ribonuclease type III [Source:NCBI gene;Acc:324724]) HSP 1 Score: 123.25 bits (308), Expect = 1.435e-28 Identity = 167/637 (26.22%), Postives = 279/637 (43.80%), Query Frame = 2 Query: 1190 KINKAFDFINSNFIIGHSTSQ-YPKKL--QMSVTKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHETQKTEAQIQKFNLINEEL------NKILKSR------VIEDELNKDDSERN-----VTPSEDDKYDYVP-YGENGPRITLNSASSVLNRYLGKLRCNSISE--PKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRR-AKQFVNLEACKILHKYDELDSSLLPYNR-----------FDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFL--KLGINDISMF---DVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKF-EYNLPVNSINYSLLLKCHKILFNIYSERIRRFDK-NIFFDPSKAKSSIILTIL------KDNKIDFNKIKEIITNPIVDNLIKNDQS----------SWVGSVIRPNYIT-NEFGAFVISKVCHDMNADSIFKND--MTYEKFLNKKYNIILSPNEKLSPLIKV------IRLTTIQSCVNKERRFLRNNRKLQRINESECY-----FVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDF 2884 K + +I+SNFI GH + P+ + KQ E+L+ F+ + N+L+AT+ +EEG+D+ CN+VV+FDPP D+RSY+QSKGRA+A S Y++ A Q+ + EL KIL+S+ +E V+ ED Y P E+G +TLNSA ++NRY KL + + PK D F + L LP+ P + + G PC + RR A++ V L C+ LHK ELD LLP + D + + YT T + P + +LY + S L +L ++ D + GIL I Q FP+Y G +V+I+ + +L+ + H+ +F+ I R +K ++ F P+ A+++ + L D IDF ++EI + + S + +VI P Y ++ F ++ V D+ S F + T+ ++ KYN+ L+ + PL+ V + L T + +N++ + L + +R + E VP L + +S+W + P+I +R++ +L+ E+ Sbjct: 8 KQDPELAYISSNFITGHGIGKNQPRSRHNEAEFRKQEEVLRKFRAHETNLLVATSAVEEGVDLPKCNLVVRFDPPADYRSYVQSKGRAQAPVSNYVML--------ADAQRMPAFHRELCTYKAIEKILRSKCSKCPEAVECGGGGIVGGGEVDALVVSCQEDLLPAYAPRQDESGASVTLNSAIGLVNRYCAKLPSDPFTHLAPKCKTLELLDG-TFCSM-LHLPINCPLRDPVVG-PCMSTRRLAEKAVALLCCERLHKAGELDDHLLPVGKETVKYEEELDLHDEEETGVPGRPGSTKRRQCYTKTIPECLNSSYPRPGSPCFLYVVGMVLSTPLPDELNFRRRRLYPPEDTARCFGILTAKRIPQ---IPCFPVYTRSGEVTVDIRLCRSCFQLTEEQLALVTRFHQYIFS----HILRLEKPDLEFQPALAEAAYCVLPLDTVHAGGDLDIDFGFMEEIELSEARTGIPAKQYSHEHPFSFLLPDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYQTFAEYYKTKYNLDLTNLNQ--PLLDVDHTSSRLNLLTPRH-LNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASMWRKAVCLPSILYRLHCLLTAEEL 623
BLAST of Endoribonuclease Dicer vs. Ensembl Sea Lamprey
Match: DDX58 (DExD/H-box helicase 58 [Source:HGNC Symbol;Acc:HGNC:19102]) HSP 1 Score: 52.373 bits (124), Expect = 6.622e-7 Identity = 97/414 (23.43%), Postives = 186/414 (44.93%), Query Frame = 2 Query: 425 LIFDEVHHLTKSDDYSQISEHVIRTTSDI-----KILGLTACLIKGVKHVKDIEFAVKQLEHKL-------NCKVITSMDNIAR--EFCG-----------NIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKL-----YLDEFLS-----CLKFINSNIESYISKSDMLMCSEKC--------KKLINLILDLKSKNEDFKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQYPKKLQMSVTKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHETQKTEAQIQKFNLINEEL 1537 +IFDE HH K+ Y+ I +H + +I+GLTA + GV K ++ A+ +H L +C + T N++ +F N+ T+ + F D I K + K+E + + + G+ EQ K ++ S++S +P K + F++ ND++F +E T+ YL++F + + E S S+ L + C KL+ L L+ + + T SIL F+ + A+ + +I + + +IG + S++ + + ++ + + + +E+ N+LIAT+V +EGLD+ CN+V+ +D + +Q++GR RA+NS+ ++ T + A+ +K NL+ E++ Sbjct: 21 IIFDECHHTCKNHPYNVIMQHYLGVKHGPSVPLSQIVGLTASV--GVGENKSLQGAI---DHVLMLCANMDSCVISTVQRNLSELAQFVPIAQHSTWASPCNLIHTVGRRRADPFAD---IIKDIMAKVEDLL-LSLNLPEKERGTQQYEQWVIKVQREG-----SMIS----IPDKELEQKFLQKY-----NDALFINEDTRAKDAMNYLEKFFHRENVFLTELVTCKREMLPSFSEKLHDLQSCLNNPENENPKLLKLEEILRDEYQMNAATKSIL-FVKTRAISEAMESWIN---EIKTELPHLKPSKLIGRAKSKHHEGMTLN-QQSGAVDEFREEQGSNLLIATSVADEGLDIQRCNLVLLYDYVGNVIKSVQTRGRGRAENSKCMLIT-TLKGGLAEKEKVNLLMEKV 405
BLAST of Endoribonuclease Dicer vs. Ensembl Yeast
Match: MPH1 (3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds flap DNA, stimulates activity of Rad27p and Dna2p; prevents crossovers between ectopic sequences by removing substrates for Mus81-Mms4 or Rad1-Rad10 cleavage; homolog of human FANCM Fanconi anemia protein that is involved in stabilizing and remodeling blocked replication forks; member of SF2 DExD/H superfamily of helicases; nonsense or missense mutations in FANCM can make people more likely to get cancer [Source:SGD;Acc:S000001441]) HSP 1 Score: 56.9954 bits (136), Expect = 2.011e-8 Identity = 43/175 (24.57%), Postives = 74/175 (42.29%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDYLNVKSDKKIFFLVKNSSLVHQHTNS-LKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDI 568 RDYQ I +K+ +N + + TG GKTFIAS ++ +Y KI F LV Q + L D+ + + + W ++ T +V + L + + L+ DE H T S Y+ + + + R S ++L LTA ++ V+++ Sbjct: 88 RDYQYTIVHKSLFQNTLCAIPTGMGKTFIASTVMLNYFRWTKKAKIIFTAPTRPLVAQQIKACLGITGIPSDQTAILLDKSRKNREEIWA---------NKRVFFATPQVVENDLKRGVLDPKDIVCLVIDEAHRATGSSAYTNVVKFIDRFNSSYRLLALTATPASDLEGVQEV 253
BLAST of Endoribonuclease Dicer vs. Ensembl Nematostella
Match: EDO35651 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SKX2]) HSP 1 Score: 105.531 bits (262), Expect = 1.332e-25 Identity = 51/93 (54.84%), Postives = 66/93 (70.97%), Query Frame = 2 Query: 3545 INDLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYL 3823 + EK + Y F NK Y++QA+TH S NR CYQRLEFLGD+++DFLVT I N L+PG++T+LR ALVNNN FA ++VK GF+K L Sbjct: 1 MQGFEKRIGYEFSNKLYLIQALTHASYSQNRLTDCYQRLEFLGDALLDFLVTELIYSRNTNLDPGELTDLRQALVNNNIFAEIAVKHGFNKSL 93 HSP 2 Score: 61.2326 bits (147), Expect = 2.438e-10 Identity = 26/55 (47.27%), Postives = 38/55 (69.09%), Query Frame = 3 Query: 3939 KVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEK 4103 KVLGDIFES+AGAIF+DS DL W VY ++ + + ++P+N + ++HEK Sbjct: 138 KVLGDIFESVAGAIFLDSGMDLVLTWRVYYRMLKPYIDAYSADVPKNPVRLIHEK 192
BLAST of Endoribonuclease Dicer vs. Ensembl Nematostella
Match: EDO36559 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SIA4]) HSP 1 Score: 86.2705 bits (212), Expect = 8.708e-23 Identity = 43/113 (38.05%), Postives = 73/113 (64.60%), Query Frame = 2 Query: 3554 LEKILNYHFKNKGYIVQAITHPS-----SIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVEEL 3877 +E L Y F+++ ++QA+TH S S N S Y++LEFLGD+++D+LVT +++ +L+PG IT+LRSA+VNN + A L+++ FHK++ +F ++ + V L Sbjct: 9 VEASLGYTFRDRDILLQALTHTSYPRAYSSVN---SSYEQLEFLGDALLDYLVTRHVLQRFPRLSPGAITDLRSAVVNNYSLACLTIQRDFHKHMRSMSMALFKVVRRFVRRL 118 HSP 2 Score: 40.4318 bits (93), Expect = 8.708e-23 Identity = 19/59 (32.20%), Postives = 33/59 (55.93%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYP 4109 V KVLGD+FE++ GA++VD D + W+V + ++ + ++P + L E P Sbjct: 147 VPKVLGDLFEALLGALYVDCGRDEELAWSVIYPLLTPIIEHYSVHLPISPTRQLVEMMP 205
BLAST of Endoribonuclease Dicer vs. Ensembl Nematostella
Match: EDO32746 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SU62]) HSP 1 Score: 90.5077 bits (223), Expect = 2.868e-18 Identity = 73/283 (25.80%), Postives = 130/283 (45.94%), Query Frame = 2 Query: 3032 MAKTCNDRTNL---EQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGIDYSIQ------------YLQWLNLP--LVPDNELNADIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANR-KFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLN 3826 + K DR+ + E+ E LGD+ L+ I SK+LF D D EG L RS L+ N++L LA L Y +++ P+ ++ N +A+C EA++G++ G+ + + Y +W+ LP + D E D +++ + + Q + + E+ F + + +A THP N QRLEFLGDSV+ F+V++++ + + G +T LRS+LVN+ + A ++ + +++N Sbjct: 523 LGKVEEDRSMIRHNERLEFLGDAVLELICSKHLFFMLPDMD-EGHLATFRSALVQNRHLALLAKMLDLSDYMQFSH--------GPDLCQEDD---------------LNHAMANCLEALLGAVYLEAGLQVATELFSRLAFTEQDLYDKWMELPKHRLQDQEPGGD---------RHLIGSSPVLQKLVEFEEGSGIPFTHIRLLARAFTHPQVGKNNLTLGDNQRLEFLGDSVLQFVVSVFLYQHFPDHHEGHLTLLRSSLVNSRSQAEIARQLNLQEFIN 772
BLAST of Endoribonuclease Dicer vs. Ensembl Nematostella
Match: EDO32367 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SV86]) HSP 1 Score: 67.0106 bits (162), Expect = 4.737e-11 Identity = 43/176 (24.43%), Postives = 81/176 (46.02%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDYLNVKSDKKIFFLVKNSSLVHQHTNSLKDYLFL-KDEIGYFYGQMENSWVDN-WTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDI 568 RDYQ I KA +N+++ L TG GKTFIA++++ +Y K+ F+ LV Q + + + + + G M + +N W ++ +T +V + L+ + V L+ DE H + Y Q+ ++ T++ ++L L+A +K V+ + Sbjct: 86 RDYQFNIVQKALYQNIMVTLPTGLGKTFIAAVVMYNYYRWYPQGKVVFMAPTKPLVAQQIEACYKIMGIPQTDTAEMTGNMAPTKRENLW---------RTKRVFFLTPQVLQNDLSRGSCAAADVVLLVVDEAHKALGNHSYCQVVREILSYTTNFRVLALSATPGDDIKAVQQV 252
BLAST of Endoribonuclease Dicer vs. Ensembl Nematostella
Match: EDO47177 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RMY2]) HSP 1 Score: 60.077 bits (144), Expect = 6.060e-9 Identity = 71/258 (27.52%), Postives = 123/258 (47.67%), Query Frame = 2 Query: 47 RDYQIEIYNKA-KNENVIIKLNTGSGKTFIASMLIKDYLNVKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDI----LNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVI-------RTTSDIKILGLTACLIKG-VKHVKDIEFAVKQLEHKLNCK-VITSMDNI----AREFCGNIKETIINYETESFPDLVAIFKSAIEKIESFI---PVD 757 R YQ E+ KA + N +I T SGKT++A + +++L+ K + K+ F+V +LV Q T K L+L+D+ Y + S N S + ++V+TA+V L I L LS V L+FDE HH K Y++I E+ + + +I+GLTA L G K+ + + + ++ ++ + V+T D R G + T+ + + ++ A+E++E ++ PVD Sbjct: 4 RGYQKELSEKALEGHNTVICAPTNSGKTYVALNIARNHLDKKKEGKVLFIVSTVNLVQQQTERFK--LYLQDK--YIVKDISGS---NGCDIPLSGLLGSSHVVVLTAQVMLCILVNALKDGSLQLSSVSLLVFDECHHTQKDHPYNKIMENYMTLRLQPAHSMPTPQIVGLTASLGVGKAKNTLEAQHHILEVCANMDAQHVVTVQDETNLEELRLHVGTPQRTVHSVTCDRTDVFESVIGKAMEQVELYVKGTPVD 254
BLAST of Endoribonuclease Dicer vs. Ensembl Medaka
Match: dicer1 (dicer 1, ribonuclease III [Source:NCBI gene;Acc:101164359]) HSP 1 Score: 205.297 bits (521), Expect = 1.009e-52 Identity = 260/1072 (24.25%), Postives = 479/1072 (44.68%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDYLNV------KSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIARE-FCGNIKETIIN---YETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTL-KKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKS--DMLMCSEKCKKLINLILDLKS--------------KNED------------------------------FKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQYP---KKLQMSVTKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHETQKTEAQIQKFNLINEELNKILKSRVIED-ELNKDDSERNVTPSEDDKYDYVPY----GENGPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNRFDNNLVIQESNDIEIIKYE-----------KYTATKLSTKFQNL-------------SIPANKLYLYSIKYTPSVFL--KLGINDISMF---DVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFE-YNLPVNSINYSLLLKCHKILFNIYSERIRRFDKNIF-FDPSKAKSS---IILTILKDN---KIDFNKIKEI----------ITNPIVDNLIKNDQSSWVGSVIRPNYIT-NEFGAFVISKVCHDMNADSIFKND--MTYEKFLNKKYNIILSPNEKLSPLIKV------IRLTTIQSCVNKERRFLRNNRKLQRINESECY-----FVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDF 2884 R YQ+E+ A N I+ LNTGSGKTFIA +L K+ + ++ ++ FL +S Q +++ + L +G + E + W++ +WS++I+ +++VMT VFL +L ++ LS++ ++FD+ H Y ++ + T +ILGLTA ++ G ++E ++ LE L T+ D + + + +E +++ Y +S L + ++ +E+ F+ ND + S + E + + K+ LS+ +VL LG + + +L + ++ K L+ D + L+ +++ E + S + D+ + K +L+ ++ + K +N+D F N + +IF+ + + LN L+ K + +I+SNFI GHS + K++++ KQ E+L+ F+ + N+LIAT+++EEG+D+ CN+VV+FD P ++RSY+QSKGRARA S YI+ ++++T+A Q+ + + KIL+++ + E+ + D E+ V DD PY + GPR+T+N+A +NRY +L + + + +L LP+ +P IKG R A++ V L C+ LHK ELD L+P + E +KYE STK + ++P YLY I + L +L ++ D + GIL PI + FP+Y G ++ I+ + +++ L+ + H+ +F+ I R +K F P+ A S+ + L ++ D+ +DF +++I T N + +VI P Y ++ F ++ V + S F + T+ ++ KYN+ LS + PL+ V + L T + +N++ + L + +R + E VP L + +SLW + P+I +R++ +L+ E+ Sbjct: 35 RKYQVELLEAALEHNTIVCLNTGSGKTFIAVLLTKELSHQIRGSYQENARRTVFLANATSSAVQQAAAVRTHSDL--HVGEYSDPQEAT---TWSRQRWSREIADNQVLVMTCHVFLHVLTNHILPLSKINLVVFDDCHLAITEHPYCEVMKLFEEHTCGSRILGLTASILNGKCDPSELEQKIQNLERILKSSAETATDLVVLDRYACQPREVVLDCGPYMDKS--GLSSRLQAELEEALLFL-------NDCNISASREDRDPTFISKQILSDCLAVLQVLGPWCADKAAGIMVRELQKYIKHEQEELSRKFLLFTD---TILRKVHALCEEHFSPASLDLKFVTPKVLRLLEILHEYKPFERQQFESVEWYNNRNQDNYVSWSDSEDEDEDEEVEAKERPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHSIGKNQARNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPVSNYIMLA-DSERTQA-FQEDLTTYKAIEKILRNKCSKSVEVGEFDGEQVV----DDDNILPPYVLRSEDGGPRVTVNTAIGHINRYCARLPSDPFTHLAPKCKTVETGNGRFQSTLYLPINSPLRVPIKGPTMHCARLAEKAVALLCCEKLHKIGELDDHLMPVGK-------------ETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYAVPEQTYYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRTFGILTAKPIPR---IPHFPVYTRSGEVTISIELQKSGFTLSAAQLDLITRLHQYIFS----HILRLEKPALEFKPTVADSAYCVLPLNVVGDSNTLDMDFKFMEDIEKSEARTGIPTTKYTKQNPFTFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTYLTPLSKFPSPEYETFAEYYKTKYNLDLSNLNQ--PLLDVDHTSSRLNLLTPRH-LNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEEL 1060 HSP 2 Score: 127.487 bits (319), Expect = 1.536e-40 Identity = 71/206 (34.47%), Postives = 117/206 (56.80%), Query Frame = 2 Query: 3344 IKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPDNELNADIEISP--------------KLAFQNISMDTDIPQSINDL-------EKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE-ELLNNQLQ 3895 I +K++ADC EA++G LT+ G + +L L L ++P + +P K+ + + D +++ L EK +NY F+NK Y++QA TH S N CYQRLEFLGD+++D+L+T ++ + ++ +PG +T+LRSALVNN FA L+VK+ +HKY ++FH++ V+ +L N++Q Sbjct: 1544 IADKSIADCVEALLGCYLTSCGERAAQMFLCSLGLKVLPSEPGTSRASKAPSSRTSAADLSYGWLKIPPRCMLAHPDAERTLQHLISGFENFEKKINYSFQNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAISPELFHVIDDFVQFQLEKNEMQ 1749 HSP 3 Score: 60.8474 bits (146), Expect = 1.536e-40 Identity = 28/60 (46.67%), Postives = 39/60 (65.00%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQ 4112 V K +GDIFES+AGAI++DS L+ VW VY M ++KF N+PR+ + L E P+ Sbjct: 1770 VPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPE 1829 HSP 4 Score: 75.0998 bits (183), Expect = 6.288e-13 Identity = 41/102 (40.20%), Postives = 61/102 (59.80%), Query Frame = 2 Query: 3044 CNDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVV---KNSDESVEVNNSREFI 3340 +D NLE+ E+LGDSFLK + YLF D +EG+L+ RS+ +SN NL+ L +KGL + F+P N+LPP +VV K+S + V+ + E + Sbjct: 1277 ASDGFNLERLEMLGDSFLKHAITTYLFCTYPDA-HEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSSTDKVDSESKEELL 1377
BLAST of Endoribonuclease Dicer vs. Ensembl Medaka
Match: dicer1 (dicer 1, ribonuclease III [Source:NCBI gene;Acc:101164359]) HSP 1 Score: 134.035 bits (336), Expect = 1.770e-42 Identity = 71/194 (36.60%), Postives = 118/194 (60.82%), Query Frame = 2 Query: 3344 IKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPDNELNAD------IEISPKLAFQNISMDTDIPQSIN---DLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVE-ELLNNQLQ 3895 I +K++ADC EA++G LT+ G + +L L L + P + +A ++I P+ + + + I+ + EK +NY F+NK Y++QA TH S N CYQRLEFLGD+++D+L+T ++ + ++ +PG +T+LRSALVNN FA L+VK+ +HKY ++FH++ V+ +L N++Q Sbjct: 1401 IADKSIADCVEALLGCYLTSCGERAAQMFLCSLGLKVTPSSRTSAADLSYGWLKIPPRCMLAHPDAERTLQHLISGFENFEKKINYSFQNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAISPELFHVIDDFVQFQLEKNEMQ 1594 HSP 2 Score: 60.4622 bits (145), Expect = 1.770e-42 Identity = 28/60 (46.67%), Postives = 39/60 (65.00%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQ 4112 V K +GDIFES+AGAI++DS L+ VW VY M ++KF N+PR+ + L E P+ Sbjct: 1615 VPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPE 1674 HSP 3 Score: 149.058 bits (375), Expect = 1.715e-35 Identity = 144/546 (26.37%), Postives = 268/546 (49.08%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDYLNV------KSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQI-SEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIARE-FCGNIKETIIN---YETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTL-KKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKS--DMLMCSEKCKKLINLILDLKS--------------KNED------------------------------FKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQ---YPKKLQMSVTKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHETQKTEA 1501 R YQ+E+ A N I+ LNTGSGKTFIA +L K+ + ++ ++ FL +S Q +++ + L +G + E + W++ +WS++I+ +++VMT VFL +L ++ LS++ ++FD+ H Y ++ EH T +ILGLTA ++ G ++E ++ LE L T+ D + + + +E +++ Y +S L + ++ +E+ F+ ND + S + E + + K+ LS+ +VL LG + + +L + ++ K L+ D + L+ +++ E + S + D+ + K +L+ ++ + K +N+D F N + +IF+ + + LN L+ K + +I+SNFI GHS + K++++ KQ E+L+ F+ + N+LIAT+++EEG+D+ CN+VV+FD P ++RSY+QSKGRARA S YI+ ++++T+A Sbjct: 35 RKYQVELLEAALEHNTIVCLNTGSGKTFIAVLLTKELSHQIRGSYQENARRTVFLANATSSAVQQAAAVRTHSDL--HVGEYSDPQEAT---TWSRQRWSREIADNQVLVMTCHVFLHVLTNHILPLSKINLVVFDDCHLAITEHPYCEVMKEH----TCGSRILGLTASILNGKCDPSELEQKIQNLERILKSSAETATDLVVLDRYACQPREVVLDCGPYMDKS--GLSSRLQAELEEALLFL-------NDCNISASREDRDPTFISKQILSDCLAVLQVLGPWCADKAAGIMVRELQKYIKHEQEELSRKFLLFTD---TILRKVHALCEEHFSPASLDLKFVTPKVLRLLEILHEYKPFERQQFESVEWYNNRNQDNYVSWSDSEDEDEDEEVEAKERPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHSIGKNQARNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPVSNYIMLA-DSERTQA 558 HSP 4 Score: 74.3294 bits (181), Expect = 9.416e-13 Identity = 40/105 (38.10%), Postives = 59/105 (56.19%), Query Frame = 2 Query: 3044 CNDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKN 3358 +D NLE+ E+LGDSFLK + YLF D +EG+L+ RS+ +SN NL+ L +KGL + F+P N+LPP +VV S + +S +L + Sbjct: 1136 ASDGFNLERLEMLGDSFLKHAITTYLFCTYPDA-HEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSSTDKVDSEVGLLTNGRT 1239
BLAST of Endoribonuclease Dicer vs. Ensembl Medaka
Match: drosha (drosha ribonuclease III [Source:NCBI gene;Acc:101175032]) HSP 1 Score: 79.337 bits (194), Expect = 2.668e-14 Identity = 72/273 (26.37%), Postives = 124/273 (45.42%), Query Frame = 2 Query: 3065 EQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGID----------YSIQYLQ--WLNLPLVP--------DNELNADIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANR-KFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKY 3820 E+ E LGD+ ++F+TS +L++ + EG L R+ ++ N++L LA + LD++ Y + P + + SD +A+C EA+IG++ G+D ++ + L+ WLN P P D +L IE SP L Q + E + F + + +A T + N QR+EFLGDS++ + T Y+ ++ + G +T LRS+LVNN T A ++ + G ++ Sbjct: 909 ERLEFLGDAVVEFLTSVHLYYLFPSLE-EGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAH-GPD--------LCRESD--------------LRHAMANCFEALIGAVYLEGGLDEARLLFGRLLFNSEDLREVWLNYPAHPLQVQEPQTDRQL---IETSPVL------------QKLTSFEDSIGVFFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEF 1142
BLAST of Endoribonuclease Dicer vs. Ensembl Medaka
Match: drosha (drosha ribonuclease III [Source:NCBI gene;Acc:101175032]) HSP 1 Score: 79.337 bits (194), Expect = 2.668e-14 Identity = 72/273 (26.37%), Postives = 124/273 (45.42%), Query Frame = 2 Query: 3065 EQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGID----------YSIQYLQ--WLNLPLVP--------DNELNADIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANR-KFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKY 3820 E+ E LGD+ ++F+TS +L++ + EG L R+ ++ N++L LA + LD++ Y + P + + SD +A+C EA+IG++ G+D ++ + L+ WLN P P D +L IE SP L Q + E + F + + +A T + N QR+EFLGDS++ + T Y+ ++ + G +T LRS+LVNN T A ++ + G ++ Sbjct: 909 ERLEFLGDAVVEFLTSVHLYYLFPSLE-EGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAH-GPD--------LCRESD--------------LRHAMANCFEALIGAVYLEGGLDEARLLFGRLLFNSEDLREVWLNYPAHPLQVQEPQTDRQL---IETSPVL------------QKLTSFEDSIGVFFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEF 1142
BLAST of Endoribonuclease Dicer vs. Ensembl Medaka
Match: drosha (drosha ribonuclease III [Source:NCBI gene;Acc:101175032]) HSP 1 Score: 79.337 bits (194), Expect = 2.668e-14 Identity = 72/273 (26.37%), Postives = 124/273 (45.42%), Query Frame = 2 Query: 3065 EQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGID----------YSIQYLQ--WLNLPLVP--------DNELNADIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANR-KFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKY 3820 E+ E LGD+ ++F+TS +L++ + EG L R+ ++ N++L LA + LD++ Y + P + + SD +A+C EA+IG++ G+D ++ + L+ WLN P P D +L IE SP L Q + E + F + + +A T + N QR+EFLGDS++ + T Y+ ++ + G +T LRS+LVNN T A ++ + G ++ Sbjct: 909 ERLEFLGDAVVEFLTSVHLYYLFPSLE-EGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAH-GPD--------LCRESD--------------LRHAMANCFEALIGAVYLEGGLDEARLLFGRLLFNSEDLREVWLNYPAHPLQVQEPQTDRQL---IETSPVL------------QKLTSFEDSIGVFFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEF 1142
BLAST of Endoribonuclease Dicer vs. Planmine SMEST
Match: SMESG000073604.1 (SMESG000073604.1) HSP 1 Score: 2480.67 bits (6428), Expect = 0.000e+0 Identity = 1257/1257 (100.00%), Postives = 1257/1257 (100.00%), Query Frame = 2 Query: 140 MLIKDYLNVKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIAREFCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKSDMLMCSEKCKKLINLILDLKSKNEDFKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQYPKKLQMSVTKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSERNVTPSEDDKYDYVPYGENGPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNRFDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFLKLGINDISMFDVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFEYNLPVNSINYSLLLKCHKILFNIYSERIRRFDKNIFFDPSKAKSSIILTILKDNKIDFNKIKEIITNPIVDNLIKNDQSSWVGSVIRPNYITNEFGAFVISKVCHDMNADSIFKNDMTYEKFLNKKYNIILSPNEKLSPLIKVIRLTTIQSCVNKERRFLRNNRKLQRINESECYFVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDFAHDLEFMNLIENSDCFEINFDDCYNIENIESSIYDPNVLKFINNIFPCFMAKTCNDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPDNELNADIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVEELLNNQLQCEHSY 3910 MLIKDYLNVKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTKDKWSKKISKYKIIVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIAREFCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKSDMLMCSEKCKKLINLILDLKSKNEDFKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQYPKKLQMSVTKQLEILKLFKEKKFNILIATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSERNVTPSEDDKYDYVPYGENGPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNRFDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFLKLGINDISMFDVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFEYNLPVNSINYSLLLKCHKILFNIYSERIRRFDKNIFFDPSKAKSSIILTILKDNKIDFNKIKEIITNPIVDNLIKNDQSSWVGSVIRPNYITNEFGAFVISKVCHDMNADSIFKNDMTYEKFLNKKYNIILSPNEKLSPLIKVIRLTTIQSCVNKERRFLRNNRKLQRINESECYFVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDFAHDLEFMNLIENSDCFEINFDDCYNIENIESSIYDPNVLKFINNIFPCFMAKTCNDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPDNELNADIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVEELLNNQLQCEHSY Sbjct: 1 MLIKDYLNVKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTKDKWSKKISKYKIIVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIAREFCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKSDMLMCSEKCKKLINLILDLKSKNEDFKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQYPKKLQMSVTKQLEILKLFKEKKFNILIATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSERNVTPSEDDKYDYVPYGENGPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNRFDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFLKLGINDISMFDVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFEYNLPVNSINYSLLLKCHKILFNIYSERIRRFDKNIFFDPSKAKSSIILTILKDNKIDFNKIKEIITNPIVDNLIKNDQSSWVGSVIRPNYITNEFGAFVISKVCHDMNADSIFKNDMTYEKFLNKKYNIILSPNEKLSPLIKVIRLTTIQSCVNKERRFLRNNRKLQRINESECYFVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDFAHDLEFMNLIENSDCFEINFDDCYNIENIESSIYDPNVLKFINNIFPCFMAKTCNDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPDNELNADIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVVEELLNNQLQCEHSY 1257 HSP 2 Score: 100.138 bits (248), Expect = 8.226e-21 Identity = 49/56 (87.50%), Postives = 50/56 (89.29%), Query Frame = 3 Query: 3885 ISCNASIHIKSEQYKVVVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLK 4052 + C S KSEQYKVVVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLK Sbjct: 1251 LQCEHSYLRKSEQYKVVVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLK 1306
BLAST of Endoribonuclease Dicer vs. Planmine SMEST
Match: SMESG000073604.1 (SMESG000073604.1) HSP 1 Score: 2431.37 bits (6300), Expect = 0.000e+0 Identity = 1234/1235 (99.92%), Postives = 1235/1235 (100.00%), Query Frame = 2 Query: 140 MLIKDYLNVKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIAREFCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKSDMLMCSEKCKKLINLILDLKSKNEDFKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQYPKKLQMSVTKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSERNVTPSEDDKYDYVPYGENGPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNRFDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFLKLGINDISMFDVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFEYNLPVNSINYSLLLKCHKILFNIYSERIRRFDKNIFFDPSKAKSSIILTILKDNKIDFNKIKEIITNPIVDNLIKNDQSSWVGSVIRPNYITNEFGAFVISKVCHDMNADSIFKNDMTYEKFLNKKYNIILSPNEKLSPLIKVIRLTTIQSCVNKERRFLRNNRKLQRINESECYFVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDFAHDLEFMNLIENSDCFEINFDDCYNIENIESSIYDPNVLKFINNIFPCFMAKTCNDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPDNELNADIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKM 3844 MLIKDYLNVKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTKDKWSKKISKYKIIVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIAREFCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKSDMLMCSEKCKKLINLILDLKSKNEDFKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQYPKKLQMSVTKQLEILKLFKEKKFNILIATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSERNVTPSEDDKYDYVPYGENGPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNRFDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFLKLGINDISMFDVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFEYNLPVNSINYSLLLKCHKILFNIYSERIRRFDKNIFFDPSKAKSSIILTILKDNKIDFNKIKEIITNPIVDNLIKNDQSSWVGSVIRPNYITNEFGAFVISKVCHDMNADSIFKNDMTYEKFLNKKYNIILSPNEKLSPLIKVIRLTTIQSCVNKERRFLRNNRKLQRINESECYFVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDFAHDLEFMNLIENSDCFEINFDDCYNIENIESSIYDPNVLKFINNIFPCFMAKTCNDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPDNELNADIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSK+ Sbjct: 1 MLIKDYLNVKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMENSWVDNWTKDKWSKKISKYKIIVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQISEHVIRTTSDIKILGLTACLIKGVKHVKDIEFAVKQLEHKLNCKVITSMDNIAREFCGNIKETIINYETESFPDLVAIFKSAIEKIESFIPVDGHHSNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMFIEDLHGAVNNDSMFFDEKTKLYLDEFLSCLKFINSNIESYISKSDMLMCSEKCKKLINLILDLKSKNEDFKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQYPKKLQMSVTKQLEILKLFKEKKFNILIATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHETQKTEAQIQKFNLINEELNKILKSRVIEDELNKDDSERNVTPSEDDKYDYVPYGENGPRITLNSASSVLNRYLGKLRCNSISEPKIIYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNRFDNNLVIQESNDIEIIKYEKYTATKLSTKFQNLSIPANKLYLYSIKYTPSVFLKLGINDISMFDVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFEYNLPVNSINYSLLLKCHKILFNIYSERIRRFDKNIFFDPSKAKSSIILTILKDNKIDFNKIKEIITNPIVDNLIKNDQSSWVGSVIRPNYITNEFGAFVISKVCHDMNADSIFKNDMTYEKFLNKKYNIILSPNEKLSPLIKVIRLTTIQSCVNKERRFLRNNRKLQRINESECYFVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDFAHDLEFMNLIENSDCFEINFDDCYNIENIESSIYDPNVLKFINNIFPCFMAKTCNDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREFILIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPDNELNADIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKI 1235 HSP 2 Score: 113.62 bits (283), Expect = 7.387e-27 Identity = 54/54 (100.00%), Postives = 54/54 (100.00%), Query Frame = 3 Query: 3891 CNASIHIKSEQYKVVVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLK 4052 CNASIHIKSEQYKVVVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLK Sbjct: 1236 CNASIHIKSEQYKVVVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLK 1289 HSP 3 Score: 28.4906 bits (62), Expect = 7.387e-27 Identity = 12/13 (92.31%), Postives = 13/13 (100.00%), Query Frame = 1 Query: 4075 EIKYQCYMKNILK 4113 +IKYQCYMKNILK Sbjct: 1289 KIKYQCYMKNILK 1301
BLAST of Endoribonuclease Dicer vs. Planmine SMEST
Match: SMESG000069643.1 (SMESG000069643.1) HSP 1 Score: 160.614 bits (405), Expect = 8.299e-50 Identity = 99/298 (33.22%), Postives = 163/298 (54.70%), Query Frame = 2 Query: 3044 CNDRTNLEQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNF-VVKNSDESVEVNNS------REFILIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPDNELN--------ADIEISPKLAFQNISMDTD--------IPQSINDLEKILNYHFKNKGYIVQAITHPSSIANRKFSCYQRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNYFDSKMFHILPKVV 3868 +D NLE+ E +GDSFLK+ ++ Y F +EG+L+ RS+ +SN NL+ A +K L + FNP+ N++ P + +N D S +N + R + +KNLAD E++ G L G +++ LQW N+ +P +E +D+ I P+ + ++TD + + + LE ++ Y F+ K Y++QA+TH S N CYQRLE LGD+V+D+ +T ++ + + +PG +T+LRSALVNNN FA L V++ H+Y ++ ++ K V Sbjct: 1333 ASDFINLERMETVGDSFLKYASTVYNF-LRYPYAHEGRLSYLRSKQVSNSNLYLRAKEKNLAGFLNSQKFNPQDNWMLPGYKYTENIDVSQIINENYSNFCPRIQHFLTDKNLADVVESLTGCYLATRGEISALRLLQWFNINCIPIDEKATPGDTGKVSDLWIKPESPI-TVKLETDNDKICYNNLIKDVIGLEAVIGYRFREKAYLLQAMTHSSYYLNVFTDCYQRLELLGDAVLDYTITRFLYEDCMRHSPGDLTDLRSALVNNNIFASLVVRYNIHQYFKCMSPVLWKLIDKFV 1628 HSP 2 Score: 58.5362 bits (140), Expect = 8.299e-50 Identity = 25/61 (40.98%), Postives = 40/61 (65.57%), Query Frame = 3 Query: 3933 VVKVLGDIFESIAGAIFVDSNYDLKAVWTVYKVFMEEPLKKFFNNIPRNKISMLHEKYPQM 4115 + K LGD+FES+AGAIF+DS DL VW V+ E ++++ + IP++ + L E P++ Sbjct: 1665 IPKCLGDVFESLAGAIFLDSGMDLNIVWKVFHPLFHERIERYTSLIPKSPVRQLLEAMPEI 1725 HSP 3 Score: 75.485 bits (184), Expect = 3.415e-13 Identity = 135/608 (22.20%), Postives = 248/608 (40.79%), Query Frame = 2 Query: 1361 LDVSHCNIVVKFDPPQDFRSYLQSKGRA-----------RAQNSQYIVFTHETQKTEAQIQKFNLINEE-----LNKILKSRVIE----DELNKDDSERNVTPSEDDKYDYVPY---------------GENGPRITLNSASSVLNRYLGKLRCNSISE--PKI-IYTYFQDEFNFHHVSLLLPMPAPYLEEIKGLPCSNKRRAKQFVNLEACKILHKYDELDSSLLPYNRFDNNLV--IQESNDIE-------------IIKYEKYTATKLSTKFQNLSIPANKLYLYSI-----KYTPSVFLKLGINDISMFDVSTYLGILLPNPIHQNKDFMKFPIYLVGGMTSVEIKFEYNLPVNSIN---------YSLLLKCHKILFNIYSERIRRFDKNIFFDPSKAKSSIILTILKDN---KIDFNKIKEII-------------TNPIVDNLIKNDQSSWVGSVIRPNYIT-NEFGAFVISKVCHDMNADSIFKND--MTYEKFLNKKYNIILSPNEK--LSPLIKVIRLT-TIQSCVN-----------KERRFLRNNRKLQRINESECYFVPSLTECISLSSSLWTQLCIFPTIHHRMNQILSIEDF 2884 D+ CN+V++FDP + + Y K R +A +S +H + +++A++ ++I++E L+ + R +E + +NK S ++ DD + P G + +TL+ A +++N Y +L +S ++ P++ I + + L LP+ +P+ KG AK L+ K L+ ELD+ P+N+ DN V I ESN ++ YE+ A +ST + + P Y+YSI + P G + D + GI+ +KFPI+ G V+IK LP+NS + + +L+ H IL I S + DK + D A ++L L N +D+ I +I T + + + ++ + +V+ P+Y + + ++ +C +M + F + T+ ++ KKY ++ ++ L V+RL TI VN +++F R + N + + +P L ++ W + PTI HR+NQ+L E+ Sbjct: 587 FDLQKCNLVIRFDPVLNLQQYFSCKSRVGIITNAASMLRKANSSVADAISHLSDRSKAKL--IHMISKEDKHSILSHLEDYRKVETGIINRINKVSSHKS-----DDHMNQNPVVNIDVNSLENPIQSTGIDSQEVTLSQAIALVNLYCARLPTDSFTKLTPELSIECDKNSQKALYRCQLRLPINSPHRLVTKGKWMPTISTAKMSSALQTLKQLYDLRELDNHFYPWNKEDNREVVSIAESNKLDENIGGMLMGSSKCRYCYERQFAKCISTTPISPNTPC---YMYSINLELSRKIPDEQNLKGRKIMISADEAKDFGIICGQTGLDKT--LKFPIFTRSGEEEVQIK--QLLPLNSCDETWTFNEKEFQKILEFHHIL--ITSVFRLKLDKGMVLDLEDAVIKLLLVPLNKNVPGHVDWEFIDLVIRSYNSVENVCRLETRRKPNGMFEFNELDYQNAVVMPSYRNFDHRQYYYVADICWNMRPTTDFPGEHFSTFAEYYTKKYGAKITNYQQPLLDADFSVMRLNLTIPRHVNFKGVPLPVPSAAKKKFFRES------NSQKQFLIPELCYIHPFPATFWHKAASMPTIMHRINQLLLAEEL 1172
BLAST of Endoribonuclease Dicer vs. Planmine SMEST
Match: SMESG000016950.1 (SMESG000016950.1) HSP 1 Score: 85.5001 bits (210), Expect = 2.019e-16 Identity = 146/573 (25.48%), Postives = 257/573 (44.85%), Query Frame = 2 Query: 47 RDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDYLNVKSDKKIFFLVKNSSLVHQHTNSLKDYLFLKDEIGYFYGQMEN--------SWVDNWTXXXXXXXXXXXXXXVMTAKVFLDILNHNKYNLSQVCYLIFDEVHHLTKSDDYSQI-------SEHVIR------TTSDI-------------------KILGLTACLIKGVKHVKDIEFAVKQLEHKL-NCKVITSMDNIAR--EFCG--NIKETIINYETESFPDLVAIFKS----AIEKIESFIPVDGHH---SNDNSGSLNAEQNNFKTLKKYLSESFSVLSTLGLVPCKLITDMF-IEDLHGAVNN----------DSMFFDEKTKLYLDEFLSCLKFINSNIESYISKSDMLMCSEKCKKLINLIL-DLKSKNEDFKNTISILIFINEKIHVLALNVLLGILPKINKAFDFINSNFIIGHSTSQYPKKLQ-MSVTKQXXXXXXXXXXXXXXXXATNVIEEGLDVSHCNIVVKFDPPQDFRSYLQSKGRARAQNSQYIVFTHETQKTEAQIQKFNLINEELNKILKSRVIED 1570 R YQ+EIY KA ++ +I L TG+GKTFIA+ML+ L ++ FLV LV Q + S K F + F Q + S + I + +I+ TA F +ILN + + ++ DE HH K Y+++ +HV+ + SDI KI+GLTA + K E +K L H L + ++T +NI EF N++ I++Y ES DL + + ++K E + G+ S+D S ++ E NN K S+ +L + + ++ + + D+ A++N D + +E ++ D + K I+ + +L ++ +IN I+ + KS ++ + +IL+ + E+ L K F+F I+GH K + MSV +Q + L +EKK I+IAT+V++EG+D+ C++V+ +PP ++ +Q +GRAR ++S+ ++ + QKT + + E +NK ++ + E+ Sbjct: 278 RKYQLEIYEKANGKDCVIFLPTGTGKTFIAAMLMLKMLEENPFHRVAFLVDKVLLVMQQSKSFKGQ-FEDICLERFESQEKTVTRKLKLISLANELIDSDHKCSIRDHDVIIATADFFKNILNKQQIYWTDFSLVVLDEAHHCVKEHPYNKLLFEYHLYPQHVLLNKNELLSVSDIQSPPEIISSELEEWKIRRCKIIGLTASPSGELDFDKTYEL-LKALLHSLGDVGIVTVTENIDELNEFTSLTNMESRIVSY-GESEKDLRKLMTTNFVFLLKKFERRVNGIGNGLILSSDTSLTI-EEINNLK------SQLSGLLVDVQEIQKSILNHILALVDIIEAIDNIGYTIAKKKFDEILENENETVWYDNNIFNFKTISKEFQ-------LLHQEQENSSIINEIVKECKSLQDENR---TILVLVEERRDAREYK---NSLEKEKLNFEF---QLIVGHGGKGAKSKHKGMSVGEQKKALDFIREKKSQIIIATSVVDEGIDLPQCDLVITVNPPASVQALVQIRGRARKKDSRLLMLCRD-QKTIDILHELMKKEENINKAIEKLISEN 823
BLAST of Endoribonuclease Dicer vs. Planmine SMEST
Match: SMESG000014289.1 (SMESG000014289.1) HSP 1 Score: 73.9442 bits (180), Expect = 8.329e-13 Identity = 73/264 (27.65%), Postives = 123/264 (46.59%), Query Frame = 2 Query: 3065 EQFEILGDSFLKFITSKYLFHFSNDTDNEGKLTQKRSQLISNKNLFHLATQKGLDKYFCYTNFNPKYNYLPPNFVVKNSDESVEVNNSREF----ILIKNKNLADCCEAIIGSILTNYGIDYSIQYLQWLNLPLVPDNE--LNADIEISPKLAFQNISMDTDIPQSINDLEKILNYHFKNKGYIVQAITHPSSIANRKFSCY-----QRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYL 3823 E+ E LGD+ ++ ITS +LF+ + +EG L R L+ N L L+ + G Y YT+ N ++Y D ++ S F I D + I GS L + D I + W +PL P E LN D +++ ++ + +++ LE L FK+ + +A+T I ++ + QRLEFLGDS++ +L T Y+ + + + G I+ LR+ LVN T A + + G +++ Sbjct: 1104 ERLECLGDAVIELITSVHLFYMFPEL-SEGHLDAFRQSLVQNHYLAELSVRLGFHNYILYTH-NMDFSY----------DSTITHARSDGFEAVMAAITLDGGLDEADRIFGSAL--FADDPRIHKV-WCEIPLHPLQEQFLNGD---------RHVIRNSPLFENLTKLEDRLGIRFKHIRLLAKAMT----IRKTGYNLFTLGDNQRLEFLGDSLLKYLTTDYLFKHFPRHHEGHISLLRNTLVNRYTQATVCTELGLDEFI 1339 HSP 2 Score: 53.9138 bits (128), Expect = 1.246e-6 Identity = 33/112 (29.46%), Postives = 59/112 (52.68%), Query Frame = 2 Query: 3578 FKNKGY--IVQAITHPSSIANRKFSCY--QRLEFLGDSVIDFLVTIYIMLNNKKLNPGKITNLRSALVNNNTFAYLSVKFGFHKYLNY-------FDSKMFHILPKVVEELL 3880 F+ KG +VQ ++H + + + +RLE LGD+VI+ + ++++ +L+ G + R +LV N+ A LSV+ GFH Y+ Y +DS + H E ++ Sbjct: 1075 FRKKGLSKMVQVMSHLPYWHETRSNIFGNERLECLGDAVIELITSVHLFYMFPELSEGHLDAFRQSLVQNHYLAELSVRLGFHNYILYTHNMDFSYDSTITHARSDGFEAVM 1186 The following BLAST results are available for this feature:
BLAST of Endoribonuclease Dicer vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Endoribonuclease Dicer vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Endoribonuclease Dicer vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 4
BLAST of Endoribonuclease Dicer vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Endoribonuclease Dicer vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Endoribonuclease Dicer vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Endoribonuclease Dicer vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Endoribonuclease Dicer vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Endoribonuclease Dicer vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Endoribonuclease Dicer vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 2
BLAST of Endoribonuclease Dicer vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of Endoribonuclease Dicer vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Endoribonuclease Dicer vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Endoribonuclease Dicer vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30016368 ID=SMED30016368|Name=Endoribonuclease Dicer|organism=Schmidtea mediterranea sexual|type=transcript|length=4352bpback to top protein sequence of SMED30016368-orf-1 >SMED30016368-orf-1 ID=SMED30016368-orf-1|Name=SMED30016368-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1294bp MKTRDYQIEIYNKAKNENVIIKLNTGSGKTFIASMLIKDYLNVKSDKKIFback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|