5'-nucleotidase protein

Overview
Name5'-nucleotidase protein
Smed IDSMED30016363
Length (bp)2652
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 5'-nucleotidase protein (SMED30016363) t-SNE clustered cells

Violin plots show distribution of expression levels for 5'-nucleotidase protein (SMED30016363) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 5'-nucleotidase protein (SMED30016363) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 5'-nucleotidase protein (SMED30016363) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30016363

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30016363h1SMcG0001475 dd_Smed_v4_3474_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30016363h1SMcG0001475 dd_Smed_v4_3474_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30016363h1SMcG0001475 dd_Smed_v4_3474_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016363h1SMcG0001475 dd_Smed_v4_3474_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30016363h1SMcG0001475 dd_Smed_v4_3474_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016363h1SMcG0001475 Contig39828newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016363h1SMcG0001475 Contig39828uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30016363h1SMcG0001475 OX_Smed_1.0.08018ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of 5'-nucleotidase protein vs. Ensembl Human
Match: NT5E (5'-nucleotidase ecto [Source:HGNC Symbol;Acc:HGNC:8021])

HSP 1 Score: 108.997 bits (271), Expect = 5.628e-24
Identity = 122/515 (23.69%), Postives = 231/515 (44.85%), Query Frame = 2
Query:   71 ELIVLHFNDVYNVEGYPNEPQA----------GAARFCTALRDSRKNCESLTLF-SGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQ-CVEKMNFQWLNSNVY--DSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQ-----------------VEFTDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDII----LTAVD------ADIALINSGTLRTD-GIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRV---DVNSIKIQ---NELIRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVAVQNYLRAIQVI 1471
            EL +LH NDV++     +E  +          G AR  T ++  R+   ++ L  +GD    ++  T+ +G  +   +N    D    GNH+FD GV+ L++  +++  F  L++N+     +  ++    + YK+       +GI+G    +    L+    N V   +   +  E+  +L+    N IIAL H  +  D+++ + V  +D+V+GGH + F                  +  +DD +++ +     F           +    + S     + ++S   ++  +   +     +L+     ++G   V LD    S R +E N+GN ICD +    L   D        + ++N G +R+     + G IT+ +L  +LP      ++ + G+ L +  E+ V +Y +  G F QVGGI   +  S+ PG RV   DV   K +    + +++D+ YK+ +  +LANG DGF ++ +  ++  D     +NV V  Y+  ++VI
Sbjct:   28 ELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKE-TPFLSNPGTNLVFEDEITALQPEVD-KLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKD-ELLRHDSGDQDINV-VSTYISKMKVI 538          
BLAST of 5'-nucleotidase protein vs. Ensembl Human
Match: NT5E (5'-nucleotidase ecto [Source:HGNC Symbol;Acc:HGNC:8021])

HSP 1 Score: 59.6918 bits (143), Expect = 1.868e-8
Identity = 79/362 (21.82%), Postives = 152/362 (41.99%), Query Frame = 2
Query:   71 ELIVLHFNDVYNVEGYPNEPQA----------GAARFCTALRDSRKNCESLTLF-SGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQ-CVEKMNFQWLNSNVY--DSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQ-----------------VEFTDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTAVDADIALINSGTLRTD 1063
            EL +LH NDV++     +E  +          G AR  T ++  R+   ++ L  +GD    ++  T+ +G  +   +N    D    GNH+FD GV+ L++  +++  F  L++N+     +  ++    + YK+       +GI+G    +    L+    N V   +   +  E+  +L+    N IIAL H  +  D+++ + V  +D+V+GGH + F                  +  +DD +++ +     F           +    + S     + ++S   ++  +   +     +L+     ++G   V LD    S R +E N+GN ICD          A+IN+    TD
Sbjct:   28 ELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKE-TPFLSNPGTNLVFEDEITALQPEVD-KLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICD----------AMINNNLRHTD 377          
BLAST of 5'-nucleotidase protein vs. Ensembl Human
Match: NT5E (5'-nucleotidase ecto [Source:HGNC Symbol;Acc:HGNC:8021])

HSP 1 Score: 50.447 bits (119), Expect = 2.106e-6
Identity = 34/119 (28.57%), Postives = 58/119 (48.74%), Query Frame = 2
Query:  914 KIGTISVDLDARFSSIRSQETNIGNFICDII----LTAVD------ADIALINSGTLRTD-GIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIE 1237
            ++G   V LD    S R +E N+GN ICD +    L   D        + ++N G +R+     + G IT+ +L  +LP      ++ + G+ L +  E+ V +Y +  G F QVG ++
Sbjct:   34 ELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGDLK 152          
BLAST of 5'-nucleotidase protein vs. Ensembl Human
Match: NT5E (5'-nucleotidase ecto [Source:HGNC Symbol;Acc:HGNC:8021])

HSP 1 Score: 51.2174 bits (121), Expect = 3.638e-6
Identity = 55/222 (24.77%), Postives = 105/222 (47.30%), Query Frame = 2
Query:   71 ELIVLHFNDVYNVEGYPNEPQA----------GAARFCTALRDSRKNCESLTLF-SGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQ-CVEKMNFQWLNSNVYDS--MTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDF 694
            EL +LH NDV++     +E  +          G AR  T ++  R+   ++ L  +GD    ++  T+ +G  +   +N    D    GNH+FD GV+ L++  +++  F  L++N+     +  ++    + YK+       +GI+G    +    L+    N V   +   +  E+  +L+    N IIAL H  +  D+++ + V  +D+V+GGH + F
Sbjct:   28 ELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKE-TPFLSNPGTNLVFEDEITALQPEVD-KLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTF 247          
BLAST of 5'-nucleotidase protein vs. Ensembl Fly
Match: CG11883 (gene:FBgn0033538 transcript:FBtr0088283)

HSP 1 Score: 424.091 bits (1089), Expect = 1.819e-136
Identity = 226/555 (40.72%), Postives = 338/555 (60.90%), Query Frame = 2
Query:   74 LIVLHFNDVYNVEGYP-NEPQAGAARFCTALRDSRKNCESLTLFSGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQCVEKMNFQWLNSNVYDSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVEFTDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTAVDADIALINSGTLRTDGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQNELIRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVAVQNYLRAIQVINGFKC-QKYRYRSSVVSMHQRKSIVMENSGLDF---------------QNLRLTPNRWGKV--KSQITQTEKERCTIGPKIEGRIVRI 1681
            L +LH+NDVYN+E     EP  GAARF TA++ S  +   L LFSGD  SPSM+ST TQG+ M  +LN      AV+GNHDFD G+D L++ +++  F WL SNV D+ T      G          + IG+IGL+E +W+ TL T+DPN+V   D+ E GN+LA +LRNEGC+LIIALTHMR  ND  L +    ID++LGGHDH  +V  T+   ++++KSGTDF+    I I  +  ++   + +V+   V +   ++ ++   +   +  +E ++   +G  SV+LD RFS +R+QETN+GN++CD++L AV AD+ ++N GT R+D +   G  T  DL+N++P+ DPL++L + G  L + LENGVS YPKLEGRFPQV GI F F     PG R+D   I++ +E + L+QSYK+CVK Y+  G DG+ +  +  V+MDD+  P L + +QN+ +A   IN  KC Q  ++R S+V++ +R S+V     +D                +++ LT     K+  ++ +   E+  C + P++E RIV I
Sbjct:  137 LTILHYNDVYNIESMAETEPVGGAARFATAIK-SFAHLNPLVLFSGDAFSPSMLSTFTQGEQMIPVLNTVGTHCAVFGNHDFDHGLDVLVKLIKQTEFPWLMSNVVDNETGRPLGGGKISHFILHNQISIGLIGLVEREWLETLPTIDPNEVTYIDYVEAGNKLARELRNEGCDLIIALTHMRTPNDINLAEKCNGIDIILGGHDHVREV--TEINGKMIVKSGTDFQQFSVITIERDAANREHFTTDVKCFDVTAKIPEDPELKQELSKYAKFIESKLSDVMGVFSVELDGRFSRVRTQETNLGNWVCDVVLAAVGADVVILNGGTFRSDRVHPVGAFTMGDLVNVIPMRDPLILLEVKGKILWQALENGVSAYPKLEGRFPQVSGISFAFDPQAEPGKRIDPQLIQVGDEYLNLEQSYKLCVKSYIFMGCDGYTMFKDATVLMDDDACPELGITLQNHFKA---INSRKCGQNTKHRQSLVTLSRRHSLVQCLDSMDLDGPSPIRKLSVGHHNKSMDLTHGNSQKMLRRASLDDLEQSTCDLAPQLEHRIVMI 685          
BLAST of 5'-nucleotidase protein vs. Ensembl Fly
Match: CG11883 (gene:FBgn0033538 transcript:FBtr0088284)

HSP 1 Score: 424.091 bits (1089), Expect = 3.324e-136
Identity = 226/555 (40.72%), Postives = 338/555 (60.90%), Query Frame = 2
Query:   74 LIVLHFNDVYNVEGYP-NEPQAGAARFCTALRDSRKNCESLTLFSGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQCVEKMNFQWLNSNVYDSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVEFTDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTAVDADIALINSGTLRTDGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQNELIRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVAVQNYLRAIQVINGFKC-QKYRYRSSVVSMHQRKSIVMENSGLDF---------------QNLRLTPNRWGKV--KSQITQTEKERCTIGPKIEGRIVRI 1681
            L +LH+NDVYN+E     EP  GAARF TA++ S  +   L LFSGD  SPSM+ST TQG+ M  +LN      AV+GNHDFD G+D L++ +++  F WL SNV D+ T      G          + IG+IGL+E +W+ TL T+DPN+V   D+ E GN+LA +LRNEGC+LIIALTHMR  ND  L +    ID++LGGHDH  +V  T+   ++++KSGTDF+    I I  +  ++   + +V+   V +   ++ ++   +   +  +E ++   +G  SV+LD RFS +R+QETN+GN++CD++L AV AD+ ++N GT R+D +   G  T  DL+N++P+ DPL++L + G  L + LENGVS YPKLEGRFPQV GI F F     PG R+D   I++ +E + L+QSYK+CVK Y+  G DG+ +  +  V+MDD+  P L + +QN+ +A   IN  KC Q  ++R S+V++ +R S+V     +D                +++ LT     K+  ++ +   E+  C + P++E RIV I
Sbjct:  137 LTILHYNDVYNIESMAETEPVGGAARFATAIK-SFAHLNPLVLFSGDAFSPSMLSTFTQGEQMIPVLNTVGTHCAVFGNHDFDHGLDVLVKLIKQTEFPWLMSNVVDNETGRPLGGGKISHFILHNQISIGLIGLVEREWLETLPTIDPNEVTYIDYVEAGNKLARELRNEGCDLIIALTHMRTPNDINLAEKCNGIDIILGGHDHVREV--TEINGKMIVKSGTDFQQFSVITIERDAANREHFTTDVKCFDVTAKIPEDPELKQELSKYAKFIESKLSDVMGVFSVELDGRFSRVRTQETNLGNWVCDVVLAAVGADVVILNGGTFRSDRVHPVGAFTMGDLVNVIPMRDPLILLEVKGKILWQALENGVSAYPKLEGRFPQVSGISFAFDPQAEPGKRIDPQLIQVGDEYLNLEQSYKLCVKSYIFMGCDGYTMFKDATVLMDDDACPELGITLQNHFKA---INSRKCGQNTKHRQSLVTLSRRHSLVQCLDSMDLDGPSPIRKLSVGHHNKSMDLTHGNSQKMLRRASLDDLEQSTCDLAPQLEHRIVMI 685          
BLAST of 5'-nucleotidase protein vs. Ensembl Fly
Match: CG11883 (gene:FBgn0033538 transcript:FBtr0339461)

HSP 1 Score: 424.091 bits (1089), Expect = 1.669e-135
Identity = 226/555 (40.72%), Postives = 338/555 (60.90%), Query Frame = 2
Query:   74 LIVLHFNDVYNVEGYP-NEPQAGAARFCTALRDSRKNCESLTLFSGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQCVEKMNFQWLNSNVYDSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVEFTDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTAVDADIALINSGTLRTDGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQNELIRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVAVQNYLRAIQVINGFKC-QKYRYRSSVVSMHQRKSIVMENSGLDF---------------QNLRLTPNRWGKV--KSQITQTEKERCTIGPKIEGRIVRI 1681
            L +LH+NDVYN+E     EP  GAARF TA++ S  +   L LFSGD  SPSM+ST TQG+ M  +LN      AV+GNHDFD G+D L++ +++  F WL SNV D+ T      G          + IG+IGL+E +W+ TL T+DPN+V   D+ E GN+LA +LRNEGC+LIIALTHMR  ND  L +    ID++LGGHDH  +V  T+   ++++KSGTDF+    I I  +  ++   + +V+   V +   ++ ++   +   +  +E ++   +G  SV+LD RFS +R+QETN+GN++CD++L AV AD+ ++N GT R+D +   G  T  DL+N++P+ DPL++L + G  L + LENGVS YPKLEGRFPQV GI F F     PG R+D   I++ +E + L+QSYK+CVK Y+  G DG+ +  +  V+MDD+  P L + +QN+ +A   IN  KC Q  ++R S+V++ +R S+V     +D                +++ LT     K+  ++ +   E+  C + P++E RIV I
Sbjct:  216 LTILHYNDVYNIESMAETEPVGGAARFATAIK-SFAHLNPLVLFSGDAFSPSMLSTFTQGEQMIPVLNTVGTHCAVFGNHDFDHGLDVLVKLIKQTEFPWLMSNVVDNETGRPLGGGKISHFILHNQISIGLIGLVEREWLETLPTIDPNEVTYIDYVEAGNKLARELRNEGCDLIIALTHMRTPNDINLAEKCNGIDIILGGHDHVREV--TEINGKMIVKSGTDFQQFSVITIERDAANREHFTTDVKCFDVTAKIPEDPELKQELSKYAKFIESKLSDVMGVFSVELDGRFSRVRTQETNLGNWVCDVVLAAVGADVVILNGGTFRSDRVHPVGAFTMGDLVNVIPMRDPLILLEVKGKILWQALENGVSAYPKLEGRFPQVSGISFAFDPQAEPGKRIDPQLIQVGDEYLNLEQSYKLCVKSYIFMGCDGYTMFKDATVLMDDDACPELGITLQNHFKA---INSRKCGQNTKHRQSLVTLSRRHSLVQCLDSMDLDGPSPIRKLSVGHHNKSMDLTHGNSQKMLRRASLDDLEQSTCDLAPQLEHRIVMI 764          
BLAST of 5'-nucleotidase protein vs. Ensembl Fly
Match: CG11883 (gene:FBgn0033538 transcript:FBtr0113063)

HSP 1 Score: 424.091 bits (1089), Expect = 1.669e-135
Identity = 226/555 (40.72%), Postives = 338/555 (60.90%), Query Frame = 2
Query:   74 LIVLHFNDVYNVEGYP-NEPQAGAARFCTALRDSRKNCESLTLFSGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQCVEKMNFQWLNSNVYDSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVEFTDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTAVDADIALINSGTLRTDGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQNELIRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVAVQNYLRAIQVINGFKC-QKYRYRSSVVSMHQRKSIVMENSGLDF---------------QNLRLTPNRWGKV--KSQITQTEKERCTIGPKIEGRIVRI 1681
            L +LH+NDVYN+E     EP  GAARF TA++ S  +   L LFSGD  SPSM+ST TQG+ M  +LN      AV+GNHDFD G+D L++ +++  F WL SNV D+ T      G          + IG+IGL+E +W+ TL T+DPN+V   D+ E GN+LA +LRNEGC+LIIALTHMR  ND  L +    ID++LGGHDH  +V  T+   ++++KSGTDF+    I I  +  ++   + +V+   V +   ++ ++   +   +  +E ++   +G  SV+LD RFS +R+QETN+GN++CD++L AV AD+ ++N GT R+D +   G  T  DL+N++P+ DPL++L + G  L + LENGVS YPKLEGRFPQV GI F F     PG R+D   I++ +E + L+QSYK+CVK Y+  G DG+ +  +  V+MDD+  P L + +QN+ +A   IN  KC Q  ++R S+V++ +R S+V     +D                +++ LT     K+  ++ +   E+  C + P++E RIV I
Sbjct:  216 LTILHYNDVYNIESMAETEPVGGAARFATAIK-SFAHLNPLVLFSGDAFSPSMLSTFTQGEQMIPVLNTVGTHCAVFGNHDFDHGLDVLVKLIKQTEFPWLMSNVVDNETGRPLGGGKISHFILHNQISIGLIGLVEREWLETLPTIDPNEVTYIDYVEAGNKLARELRNEGCDLIIALTHMRTPNDINLAEKCNGIDIILGGHDHVREV--TEINGKMIVKSGTDFQQFSVITIERDAANREHFTTDVKCFDVTAKIPEDPELKQELSKYAKFIESKLSDVMGVFSVELDGRFSRVRTQETNLGNWVCDVVLAAVGADVVILNGGTFRSDRVHPVGAFTMGDLVNVIPMRDPLILLEVKGKILWQALENGVSAYPKLEGRFPQVSGISFAFDPQAEPGKRIDPQLIQVGDEYLNLEQSYKLCVKSYIFMGCDGYTMFKDATVLMDDDACPELGITLQNHFKA---INSRKCGQNTKHRQSLVTLSRRHSLVQCLDSMDLDGPSPIRKLSVGHHNKSMDLTHGNSQKMLRRASLDDLEQSTCDLAPQLEHRIVMI 764          
BLAST of 5'-nucleotidase protein vs. Ensembl Fly
Match: CG30103 (gene:FBgn0050103 transcript:FBtr0086929)

HSP 1 Score: 124.405 bits (311), Expect = 3.782e-29
Identity = 120/488 (24.59%), Postives = 208/488 (42.62%), Query Frame = 2
Query:  134 AGAARFCTALRDSRKNCESLTLF--SGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQCVEKMNFQWLNSNVYDSMTDELF--ADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDF-----------------QVEFTDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTA------------VDADIALINSGTLRTD-GIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPK-LEGRFPQVGGIEFRFWSSKPPGSRVD-----VNSIKIQN-ELIRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLN-VAVQNYLRAIQVI 1471
             G  R   A+  +R       ++   GD    +   ++ +G  +  +LN    D    G H+ D G D L + +  + F  ++SN+      +L   A+ +   +  +   KIGI+G I PD   T     P++VI K      N+   +L+ +G ++IIAL H  +  D  + K  P +D+V+GG  H F                  +    D +++ +     +           +N   + S +   + +++ +   K V D +Q     ++   +  +GT SV L+    S    E N GNFI D  + A             DA I LIN+G +R        G IT  D++ +LP    L    I+G+QL++ LE+      K +     QV G+  +F  S P G R+       +  +I + E +  +  Y + V  +L NG +G++ +   P   + EN  +L+ +AV  YL+  +VI
Sbjct:   61 GGFGRVAEAVSAARNTATDPVIYLNGGDSFQGTSWFSVYRGKMVARMLNFLAPDAMALGVHELDDGTDALAEFLNTITFPMVSSNINLINEPKLAENANLVTSLVITKGNRKIGIVGYIRPD---TKERTQPSNVIFKREVPAINKETKKLKGQGIDIIIALGHSGYEKDMEIAKRCPDVDIVVGGQSHTFLYSGKAPSKEVSEGPYPTIVVKPDGRKVPVVQAYAYTKYLGNLSLEFDNGGNLLSFKGSPILLNNRFHPRKDVQDFLQLYRQVIDDMERHVVGTTSVYLNGDRKSCGYSECNFGNFIADSFVYARVVQTMADRSSWTDASIGLINAGAIRASIDPGETGAITEADVVTVLPFSQDLYYTRISGSQLMKALEHSAQMRSKHMTSAHLQVSGLRLKFNHSLPKGERITEIRALCSDCQIPHYEAVDTNGYYGVVVTSFLLNGGEGYSFVD--PKRPEVENMTILDRMAVIQYLQEHKVI 543          
BLAST of 5'-nucleotidase protein vs. Ensembl Zebrafish
Match: nt5e (5'-nucleotidase, ecto (CD73) [Source:ZFIN;Acc:ZDB-GENE-040426-1261])

HSP 1 Score: 125.561 bits (314), Expect = 1.970e-29
Identity = 126/493 (25.56%), Postives = 215/493 (43.61%), Query Frame = 2
Query:   71 ELIVLHFNDVY-NVE------GYPNEPQ--AGAARFCTALRDSR-KNCESLTLFSGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQ-CVEKMNFQWLNSNVYDSMTDELFADGIK-----YKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVEF-----------------TDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDII----------LTAVDADIALINSGTLRT--DGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKI--------QNELIRLDQSYKICVKQYLANGKDGFNVLAE 1390
            +L +LH NDV+  VE      G  ++P   AG +R  T +++ R K    L L +GD    ++     +G      +N+   D    GNH+FD GVD L++  ++++N   L++N+     DE  A  I      YKIF     K+G++G    +  A L+   P+     +   +  ++  +L   G N IIAL H  +  D+++ K V  +D+V+GGH + F                     +DD +++ +     F           + +  +       + ++S  E +  +   V +    L      ++G   V L+  F   R +E N+GN ICD +          L        ++N G +R   D     G IT  DL+ +LP      ++ + G+ L+E  E+ V ++    G F QV G +  +  SK PGSR  V S+K+          E +  ++ YK+ +  YL +G DGF ++ E
Sbjct:   26 QLTLLHTNDVHARVEETNKDSGKCSKPPCFAGVSRRSTKIKEIRSKEKNVLLLDAGDQFQGTVWFNYYKGAEAAYFMNQLKYDAMALGNHEFDNGVDGLLKPFLQEVNCTVLSANI---KADETLAPRISGYYFPYKIFTLGSEKVGVVGYTSAETPA-LSLPGPHLKFEDEITALQPQVD-KLLTLGVNKIIALGHSGFLMDQMIAKKVQGVDVVIGGHTNTFLYTGDPPSTEVPAGPYPLMVKSDDGRQVPVVQAYAFGKYLGFLKVTFDANGNVLESTGNPILLNSSVEPDPDIQAKVDSWRMNLANYSSQQVGQTLVFLNGTFEECRFRECNLGNLICDAMVHHNIKYADELQWNHVSSCILNGGGIRAPIDERNRNGSITMEDLIAVLPFGGTFDLVQLNGSTLLEAFEHSVRRHGGNTGEFLQVSGFQVVYDLSKAPGSR--VKSVKVLCTQCRVPHYEPLVPNKVYKVVLPSYLVDGGDGFAMIKE 511          
BLAST of 5'-nucleotidase protein vs. Ensembl Xenopus
Match: NT5E (5'-nucleotidase, ecto (CD73) [Source:NCBI gene;Acc:100489347])

HSP 1 Score: 125.176 bits (313), Expect = 2.387e-29
Identity = 127/498 (25.50%), Postives = 209/498 (41.97%), Query Frame = 2
Query:   56 NTMTSELIVLHFNDVY-NVE---------GYPNEPQAGAARFCTALRDSRKNCESLTLF-SGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQ-CVEKMNFQWLNSNVYDSMTDELFADGI-----KYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVE-----------------FTDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTAV----------DADIALINSGTLRT--DGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQNEL-------IRLDQSYKICVKQYLANGKDGFNVLAE 1390
            +  T +L +LH NDV+  VE         G P +   G AR    + + R     + L  +GD    ++     +G      +N    D    GNH+FD GV  L+   ++++ F  L++N+    +D+  A  I      YKI E    KIG+IG    +    L+   P+ V   +   +  ++  +L   G N IIAL H  +  D+++ + V  ID+V+GGH + F                     +DD + + +     F           +   T+       + +D    ++  +   V      +       IG   V L+      R++E N+GN ICD ++                + ++N G +R   D  IS G IT  DL+++LP      ++ I G+ L    E+ V +Y    G F QVGGI+  F + K PG RV V    I  E        + +D+ YK+ +  Y+A G DGF++L +
Sbjct:   19 SAATLQLTILHTNDVHARVEQTNRDSGKCGDPQDCYGGVARRFAKIEEIRSTHRHVLLLDAGDQYQGTIWFNYYKGHEAAYFMNYLKYDAMALGNHEFDNGVSGLLSPFLQEVKFPILSANI---KSDKQLAPNITGYYLPYKILEVGTEKIGVIGYSSRE-TPVLSNSGPHLVFEDEVLALQPQVD-KLLTLGVNKIIALGHSGFETDKLIAQKVKGIDVVVGGHTNTFLYTGPPPSNDVPVGEYPFSVMSDDGRSVPVVQAYAFGKYLGYLNITFDEQGTVVHSFGNPILLDRSIPEDATLLGEVNQWKEAIRNYSSQPIGKTLVYLNGSSLECRNRECNMGNLICDAMIDNNIRHPDEVRWNHVSLCILNGGGIRAAIDERISNGTITTEDLISVLPFEGTFDLIEIKGSTLKSAFEHSVHRYGSGTGEFLQVGGIKVVFDTEKSPGQRV-VKLEVICTECRVPKYVPVEMDKIYKVVLPSYIAAGGDGFSMLKD 510          
BLAST of 5'-nucleotidase protein vs. Ensembl Xenopus
Match: NT5E (5'-nucleotidase, ecto (CD73) [Source:NCBI gene;Acc:100489347])

HSP 1 Score: 125.176 bits (313), Expect = 3.826e-29
Identity = 132/525 (25.14%), Postives = 219/525 (41.71%), Query Frame = 2
Query:   56 NTMTSELIVLHFNDVY-NVE---------GYPNEPQAGAARFCTALRDSRKNCESLTLF-SGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQ-CVEKMNFQWLNSNVYDSMTDELFADGI-----KYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVE-----------------FTDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTAV----------DADIALINSGTLRT--DGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQNEL-------IRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVAVQNYLRAIQVI 1471
            +  T +L +LH NDV+  VE         G P +   G AR    + + R     + L  +GD    ++     +G      +N    D    GNH+FD GV  L+   ++++ F  L++N+    +D+  A  I      YKI E    KIG+IG    +    L+   P+ V   +   +  ++  +L   G N IIAL H  +  D+++ + V  ID+V+GGH + F                     +DD + + +     F           +   T+       + +D    ++  +   V      +       IG   V L+      R++E N+GN ICD ++                + ++N G +R   D  IS G IT  DL+++LP      ++ I G+ L    E+ V +Y    G F QVGGI+  F + K PG RV V    I  E        + +D+ YK+ +  Y+A G DGF++L +  +  D  +  +    V NY+   Q +
Sbjct:   17 SAATLQLTILHTNDVHARVEQTNRDSGKCGDPQDCYGGVARRFAKIEEIRSTHRHVLLLDAGDQYQGTIWFNYYKGHEAAYFMNYLKYDAMALGNHEFDNGVSGLLSPFLQEVKFPILSANI---KSDKQLAPNITGYYLPYKILEVGTEKIGVIGYSSRE-TPVLSNSGPHLVFEDEVLALQPQVD-KLLTLGVNKIIALGHSGFETDKLIAQKVKGIDVVVGGHTNTFLYTGPPPSNDVPVGEYPFSVMSDDGRSVPVVQAYAFGKYLGYLNITFDEQGTVVHSFGNPILLDRSIPEDATLLGEVNQWKEAIRNYSSQPIGKTLVYLNGSSLECRNRECNMGNLICDAMIDNNIRHPDEVRWNHVSLCILNGGGIRAAIDERISNGTITTEDLISVLPFEGTFDLIEIKGSTLKSAFEHSVHRYGSGTGEFLQVGGIKVVFDTEKSPGQRV-VKLEVICTECRVPKYVPVEMDKIYKVVLPSYIAAGGDGFSMLKDESLKHDSGDTDL--TVVSNYIAQRQRV 533          
BLAST of 5'-nucleotidase protein vs. Ensembl Xenopus
Match: NT5E (5'-nucleotidase, ecto (CD73) [Source:NCBI gene;Acc:100489347])

HSP 1 Score: 125.176 bits (313), Expect = 3.901e-29
Identity = 132/523 (25.24%), Postives = 218/523 (41.68%), Query Frame = 2
Query:   56 NTMTSELIVLHFNDVY-NVE---------GYPNEPQAGAARFCTALRDSRKNCESLTLF-SGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQ-CVEKMNFQWLNSNVYDSMTDELFADGIK-----YKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVE-----------------FTDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTAV----------DADIALINSGTLRT--DGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQNEL-------IRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVAVQNYLRAIQ 1465
            +  T +L +LH NDV+  VE         G P +   G AR    + + R     + L  +GD    ++     +G      +N    D    GNH+FD GV  L+   ++++ F  L++N+    +D+  A  I      YKI E    KIG+IG    +    L+   P+ V   +   +  ++  +L   G N IIAL H  +  D+++ + V  ID+V+GGH + F                     +DD + + +     F           +   T+       + +D    ++  +   V      +       IG   V L+      R++E N+GN ICD ++                + ++N G +R   D  IS G IT  DL+++LP      ++ I G+ L    E+ V +Y    G F QVGGI+  F + K PG RV V    I  E        + +D+ YK+ +  Y+A G DGF++L +  +  D  +  +    V NY+   Q
Sbjct:   23 SAATLQLTILHTNDVHARVEQTNRDSGKCGDPQDCYGGVARRFAKIEEIRSTHRHVLLLDAGDQYQGTIWFNYYKGHEAAYFMNYLKYDAMALGNHEFDNGVSGLLSPFLQEVKFPILSANI---KSDKQLAPNITGYYLPYKILEVGTEKIGVIGYSSRE-TPVLSNSGPHLVFEDEVLALQPQVD-KLLTLGVNKIIALGHSGFETDKLIAQKVKGIDVVVGGHTNTFLYTGPPPSNDVPVGEYPFSVMSDDGRSVPVVQAYAFGKYLGYLNITFDEQGTVVHSFGNPILLDRSIPEDATLLGEVNQWKEAIRNYSSQPIGKTLVYLNGSSLECRNRECNMGNLICDAMIDNNIRHPDEVRWNHVSLCILNGGGIRAAIDERISNGTITTEDLISVLPFEGTFDLIEIKGSTLKSAFEHSVHRYGSGTGEFLQVGGIKVVFDTEKSPGQRV-VKLEVICTECRVPKYVPVEMDKIYKVVLPSYIAAGGDGFSMLKDESLKHDSGDTDL--TVVSNYIAQRQ 537          
BLAST of 5'-nucleotidase protein vs. Ensembl Xenopus
Match: NT5E (5'-nucleotidase, ecto (CD73) [Source:NCBI gene;Acc:100489347])

HSP 1 Score: 124.79 bits (312), Expect = 4.240e-29
Identity = 132/523 (25.24%), Postives = 218/523 (41.68%), Query Frame = 2
Query:   56 NTMTSELIVLHFNDVY-NVE---------GYPNEPQAGAARFCTALRDSRKNCESLTLF-SGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQ-CVEKMNFQWLNSNVYDSMTDELFADGI-----KYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVE-----------------FTDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTAV----------DADIALINSGTLRT--DGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQNEL-------IRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVAVQNYLRAIQ 1465
            +  T +L +LH NDV+  VE         G P +   G AR    + + R     + L  +GD    ++     +G      +N    D    GNH+FD GV  L+   ++++ F  L++N+    +D+  A  I      YKI E    KIG+IG    +    L+   P+ V   +   +  ++  +L   G N IIAL H  +  D+++ + V  ID+V+GGH + F                     +DD + + +     F           +   T+       + +D    ++  +   V      +       IG   V L+      R++E N+GN ICD ++                + ++N G +R   D  IS G IT  DL+++LP      ++ I G+ L    E+ V +Y    G F QVGGI+  F + K PG RV V    I  E        + +D+ YK+ +  Y+A G DGF++L +  +  D  +  +    V NY+   Q
Sbjct:   21 SAATLQLTILHTNDVHARVEQTNRDSGKCGDPQDCYGGVARRFAKIEEIRSTHRHVLLLDAGDQYQGTIWFNYYKGHEAAYFMNYLKYDAMALGNHEFDNGVSGLLSPFLQEVKFPILSANI---KSDKQLAPNITGYYLPYKILEVGTEKIGVIGYSSRE-TPVLSNSGPHLVFEDEVLALQPQVD-KLLTLGVNKIIALGHSGFETDKLIAQKVKGIDVVVGGHTNTFLYTGPPPSNDVPVGEYPFSVMSDDGRSVPVVQAYAFGKYLGYLNITFDEQGTVVHSFGNPILLDRSIPEDATLLGEVNQWKEAIRNYSSQPIGKTLVYLNGSSLECRNRECNMGNLICDAMIDNNIRHPDEVRWNHVSLCILNGGGIRAAIDERISNGTITTEDLISVLPFEGTFDLIEIKGSTLKSAFEHSVHRYGSGTGEFLQVGGIKVVFDTEKSPGQRV-VKLEVICTECRVPKYVPVEMDKIYKVVLPSYIAAGGDGFSMLKDESLKHDSGDTDL--TVVSNYIAQRQ 535          
BLAST of 5'-nucleotidase protein vs. Ensembl Mouse
Match: Nt5el (5' nucleotidase, ecto-like [Source:MGI Symbol;Acc:MGI:1914013])

HSP 1 Score: 405.986 bits (1042), Expect = 5.291e-131
Identity = 207/482 (42.95%), Postives = 316/482 (65.56%), Query Frame = 2
Query:   74 LIVLHFNDVYNVEGYPNEPQAGAARFCTALRDSRKNCESLTLFSGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQCVEKMNFQWLNSNVYDSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVEFTDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTAVDADIALINSGTLRTDGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQNELIRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVAVQNYLRAIQVINGFKCQKYRYRSSVVS 1519
            L +LHFNDVY+V+    EP  GAARF TA++        L +FSGDCL+PS++STIT+G HM  ILNE  +  AV+GNH+FDFGVD L + +++M+F W  SNV+D  T E    G   KI      KIG++GL+E DW+ TLATV+  +V  KD+ E  NELA++LR EG +L+IA+THM+W ND  L ++   +DL+LGGHDH++ ++  ++    ++KSG+DF+N+ KI I + + S      +VE L   S  E++  +  +V++ +  ++  ++  +  I V LD R  ++R  E+N+GN + + +L A  AD+AL+NSGTLR D I   G+ T  DL+ +LP++DP++V+  TGAQL+E LENGV +YP L+GRFPQV G+EF F     PG RV  +++K+Q + ++  + Y + +K+Y+ANGKDG+++   CP + D E A VL+  V N+  +I+++ G K     +R S+++
Sbjct:   40 LTILHFNDVYDVDSSTEEPVGGAARFATAVK-RFSILNPLLIFSGDCLNPSILSTITKGKHMISILNELGVHFAVFGNHEFDFGVDILEEYMKQMHFTWFLSNVHDRFTSEPLGHGAVKKIVNWNNRKIGLMGLVEEDWLDTLATVNKANVNYKDYVETANELAVELRAEGADLVIAMTHMKWKNDTRLAQHAEGLDLILGGHDHEYGIKKVNET--WIVKSGSDFKNLTKIDIQLFDASFQYVFEKVEIL---SYLEEDSYIKAIVRDFTQNIQYMLEEVLCPIDVALDGREITVRRCESNLGNLVTNAMLEATHADVALLNSGTLRYDRIHPPGNFTLHDLLAILPIVDPVLVVRATGAQLLEALENGVYKYPALDGRFPQVAGLEFGFDPDAEPGHRVIRDTVKVQGQYLQRKKVYLLAIKEYIANGKDGYSMFRACPRMFDPETAQVLSTVVMNHFESIKILQGKKKCLSGHRMSLIT 515          
BLAST of 5'-nucleotidase protein vs. Ensembl Mouse
Match: Nt5e (5' nucleotidase, ecto [Source:MGI Symbol;Acc:MGI:99782])

HSP 1 Score: 101.679 bits (252), Expect = 7.841e-22
Identity = 123/536 (22.95%), Postives = 235/536 (43.84%), Query Frame = 2
Query:   71 ELIVLHFNDVYN-VEGYPNEPQ---------AGAARFCTALRDSRKNCESLTLF--SGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLM-QCVEKMNFQWLNSNV--YDSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQ-----------------VEFTDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILT----------AVDADIALINSGTLRTDGIISK--GDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRV---DVNSIKIQ---NELIRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVAVQNYLRAIQVINGFKCQKYRYRSSVVSMHQ 1528
            EL +LH NDV++ +E   ++            G AR  T ++  RK  E   LF  +GD    ++  T+ +G  +   +N    D    GNH+FD GV+ L+   +  + F  L++N+     +  ++    +  K+    G  +GI+G    +    L+    N V   +   +  E+  +L+    N IIAL H  +  D+++ + V  +D+V+GGH + F                  +   DD +++ +     F           ++   + +     + ++S   ++  +   +     +L+     ++G   V LD    + R +E N+GN ICD ++                + ++N G +R+  I  K  G IT+ +L  +LP      ++ + G+ L +  E+ V +Y +  G F QVGGI   +  ++ P +RV   +V   K +    E + +D+ YK+ +  YLANG DGF ++ +  +  D  +  +    V  Y+  ++V+  +   + R + S  S +Q
Sbjct:   30 ELTILHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKVQQIRKE-EPNVLFLDAGDQYQGTIWFTVYKGLEVAHFMNILGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAHQISGLFLPSKVLSVGGEVVGIVGYTSKE-TPFLSNPGTNLVFEDEISALQPEVD-KLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDIVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTADDGRQVPVVQAYAFGKYLGYLKVEFDDKGNVITSYGNPILLNSSIPEDATIKADINQWRIKLDNYSTQELGRTIVYLDGSTQTCRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSP-IDEKNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDINRKPWNRVVQLEVLCTKCRVPIYEPLEMDKVYKVTLPSYLANGGDGFQMIKDELLKHDSGDQDI--SVVSEYISKMKVV--YPAVEGRIKFSAASHYQ 557          
BLAST of 5'-nucleotidase protein vs. UniProt/SwissProt
Match: sp|O34313|NTPES_BACSU (Trifunctional nucleotide phosphoesterase protein YfkN OS=Bacillus subtilis (strain 168) OX=224308 GN=yfkN PE=1 SV=1)

HSP 1 Score: 152.14 bits (383), Expect = 3.379e-36
Identity = 135/486 (27.78%), Postives = 208/486 (42.80%), Query Frame = 2
Query:   71 ELIVLHFNDVYNVEGYPNEPQAGAARFCTALRDSRKNC-ESLTLFSGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQCV---------------EKMNFQWLNSNVYDSMTDEL---------FADGIK-------YKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKD--FCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDF--QVEFTDDKKRLLIKSGTDFRX--XXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILT----AVDADIALINSGTLRTDGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSR---VDVNSIKIQNELIRLDQSYKICVKQYLANGKDGFNVLAEC 1393
            +L V+H ND +            AAR  T + + R     ++ L +GD  S  +  T   G    +++N    D   +GNH+FD G   L   +               E   F  +++NV  S   +L         F  G K       Y + +  G K+ + GL   D   T  T  P   IV +  F    N +      E  N IIALTH+    D  L K V  IDL++GGH H    ++E  ++++  ++    ++                   +   +  L +D   E+  +    +    +ELE     K+G   V LD +   +R++ETN+GNFI D +L     A  A IA+ N G +R    I KGDIT  +++N++P  + L V  +TG Q+ E LE G+S      G FPQV GIE+ F  +  PG R   V + S       I  D +Y++    ++  G DG++V  E 
Sbjct:  668 DLTVMHTNDTH-------AHLDDAARRMTKINEVRSETNHNILLDAGDVFSGDLYFTKWNGLADLKMMNMMGYDAMTFGNHEFDKGPTVLSDFLSGNSATVDPANRYHFEAPEFPIVSANVDVSNEPKLKSFVKKPQTFTAGEKKEAGIHPYILLDVDGEKVAVFGLTTED---TATTSSPGKSIVFNDAFETAQNTVKAIQEEEKVNKIIALTHIGHNRDLELAKKVKGIDLIIGGHTHTLVDKMEVVNNEEPTIVAQAKEYGQFLGRVDVAFDEKGVVQTDKSNLSVLPIDEHTEENPEAKQELDQFKNELEDVKNEKVGYTDVALDGQREHVRTKETNLGNFIADGMLAKAKEAAGARIAITNGGGIRAG--IDKGDITLGEVLNVMPFGNTLYVADLTGKQIKEALEQGLSNVENGGGAFPQVAGIEYTFTLNNKPGHRVLEVKIESPNGDKVAINTDDTYRVATNNFVGAGGDGYSVFTEA 1141          

HSP 2 Score: 59.3066 bits (142), Expect = 1.504e-7
Identity = 42/160 (26.25%), Postives = 70/160 (43.75%), Query Frame = 2
Query:  266 ILNEFDIDLAVYGNHDFDFGVDKLMQCVEKMNFQWLNSNVYDSMTDELFADGIKYKIFERC---------GVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHM--------RWANDRI--LFKNVPSIDLVLGGHDH 688
            ++N    D    GNH+F++G+D L   ++  +F  +N+NV  +  +  +     Y I E+           VK+G IG + P  +          V V+D  E  NE   +++ EG ++IIAL H           A + +  L      ID ++ GH H
Sbjct:  128 VMNALKYDAGTLGNHEFNYGLDFLDGTIKGADFPIVNANVKTTSGENRYT---PYVINEKTLIDENGNEQKVKVGYIGFVPPQIMTWDKKNLEGQVQVQDIVESANETIPKMKAEGADVIIALAHTGIEKQAQSSGAENAVFDLATKTKGIDAIISGHQH 284          
BLAST of 5'-nucleotidase protein vs. UniProt/SwissProt
Match: sp|A9BJC1|MGGB_PETMO (Mannosylglucosyl-3-phosphoglycerate phosphatase OS=Petrotoga mobilis (strain DSM 10674 / SJ95) OX=403833 GN=mggB PE=1 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 2.153e-27
Identity = 125/472 (26.48%), Postives = 208/472 (44.07%), Query Frame = 2
Query:   68 SELIVLHFNDVYNVEGYPNEPQAGAARFCTALRDSRKNCES-----LTLFSGDCLSPSMISTITQGDHMPEIL--NEFDIDLAVYGNHDFDFGVDKLMQCVEKMNFQWLNSN-VYDSMTDELFADGIKYKIFERCGVKIGIIGLI-------EPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRI-------------LFKNVPSIDLVLGGHDHDFQVEFTDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKE-KKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTAVDADIALINSGTLRTDGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQNELIRLDQSYKICVKQYLANGKDGFNVLAECP 1396
            + L++ H ND +    +  E   G AR  T +  +R+         L L +GD    + I    Q D +P+ L  +   +D    GNH+FD   + L +  E   F +L +N V +     +F     Y I +     +GIIGL+       EP ++     VD  + +          + + +  E  +++I L H+ +  D               L +NV  +D+++ GH H            ++ ++G +  NIG+I + I++        EV  LT D   +   K   D      SE    +   +G   V LD   + +RS ETN+ N I D ++    AD+AL+N G +R    I  G+ITYRD++ +LP  + L VL +TG  +++VL N  +  P  +G    V G+       K         ++KI  + I L+++YK+    Y+A G DG+ +LA  P
Sbjct:   22 NHLVIFHMNDTHG-HVWGTEDGGGFARAATLINQAREEVAKEGGAVLFLHAGDV--NTGIPESDQLDAVPDFLALHYMGLDAMSLGNHEFDKPFEVLEKQYEVAQFPFLGANFVNEKRGGPVFE---PYIIKDYGDFSVGIIGLVTEQTKVLEPIYLGENTIVDAEETL---------NMYLPIVQEKADVVIVLAHLGYHADGGRPNLSVEFTTSDELAENVSGVDIIIDGHSHTLLETPVVINNVIVAQAGDNAENIGRIDLWIDDGRIVDWRGEVIPLTSDIPEDPFIKMFTDAFYQLGSE---ALNEVVGVTKVYLDGERAHVRSDETNLSNLIADGMIWKTGADVALMNGGGIRAS--IEAGEITYRDILTVLPFGNTLYVLELTGKDIMDVL-NYAATIPDGQGAKLHVAGLTAEIKGGK-------ATNVKINGKPIDLNKTYKVVTNNYVAAGGDGYTMLAGKP 465          
BLAST of 5'-nucleotidase protein vs. UniProt/SwissProt
Match: sp|Q9XZ43|5NTD_LUTLO (Protein 5NUC OS=Lutzomyia longipalpis OX=7200 GN=5NUC PE=1 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 5.625e-27
Identity = 120/506 (23.72%), Postives = 215/506 (42.49%), Query Frame = 2
Query:   71 ELIVLHFNDVY----NVEGYPNEPQ----------AGAARFCTALRDSRKNCESLTLF--SGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQCVEKMNFQWLNSNVYDSMTDELFADGIKYK---IFERCGVKIGIIGLIEPD--WVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDF-----------------QVEFTDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTA------------VDADIALINSGTLRT--DGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLE--GRFPQVGGIEFRFWSSKPPGSRVDVNSIKI--------QNELIRLDQSYKICVKQYLANGKDGFNVLAECPVI 1402
            E+I+LH ND++          N+ Q           G AR  T ++  R+   S  LF  +GD  + +   T+ +     E++N    D A  GNH+FD GV+ L+  +  + F  L +N+ D+  +    +    K   IF   G ++G+IG + PD  +++ +  V+     + +   I  E     + E   +II + H     DR + +  P +D+++GGH H F                  V      K++ I     F           N    +     + + +++   ++++V   ++     +E      IG   V L+   +  R  E N+GN I D  + A             DA + L  SG +R   D   + G IT  +L N+LP  + L V+ + G  L + LE+ V +Y      G FPQV G++ RF  ++  G R  V S+K+        + + +R  ++Y + +  ++ +G DG+++     +I
Sbjct:   31 EIIILHTNDMHARFDQTNAGSNKCQEKDKIASKCYGGFARVSTMVKKFREENGSSVLFLNAGDTYTGTPWFTLYKETIATEMMNILRPDAASLGNHEFDKGVEGLVPFLNGVTFPILTANL-DTSQEPTMTNAKNLKRSMIFTVSGHRVGVIGYLTPDTKFLSDVGKVN----FIPEVEAINTEAQRLKKEENAEIIIVVGHSGLIKDREIAEKCPLVDIIVGGHSHTFLYTGSQPDREVPVDVYPVVVTQSSGKKVPIVQAYCFTKYLGYFKVTINGKGNVVGWTGQPILLNNNIPQDQEVLTALEKYRERVENYGNRVIGVSRVILNGGHTECRFHECNMGNLITDAFVYANVISTPMSTNAWTDASVVLYQSGGIRAPIDPRTAAGSITRLELDNVLPFGNALYVVKVPGNVLRKALEHSVHRYSNTSGWGEFPQVSGLKIRFNVNEEIGKR--VKSVKVLCSNCSQPEYQPLRNKKTYNVIMDSFMKDGGDGYSMFKPLKII 529          
BLAST of 5'-nucleotidase protein vs. UniProt/SwissProt
Match: sp|P21589|5NTD_HUMAN (5'-nucleotidase OS=Homo sapiens OX=9606 GN=NT5E PE=1 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 2.703e-23
Identity = 122/515 (23.69%), Postives = 231/515 (44.85%), Query Frame = 2
Query:   71 ELIVLHFNDVYNVEGYPNEPQA----------GAARFCTALRDSRKNCESLTLF-SGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQ-CVEKMNFQWLNSNVY--DSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQ-----------------VEFTDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDII----LTAVD------ADIALINSGTLRTD-GIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRV---DVNSIKIQ---NELIRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVAVQNYLRAIQVI 1471
            EL +LH NDV++     +E  +          G AR  T ++  R+   ++ L  +GD    ++  T+ +G  +   +N    D    GNH+FD GV+ L++  +++  F  L++N+     +  ++    + YK+       +GI+G    +    L+    N V   +   +  E+  +L+    N IIAL H  +  D+++ + V  +D+V+GGH + F                  +  +DD +++ +     F           +    + S     + ++S   ++  +   +     +L+     ++G   V LD    S R +E N+GN ICD +    L   D        + ++N G +R+     + G IT+ +L  +LP      ++ + G+ L +  E+ V +Y +  G F QVGGI   +  S+ PG RV   DV   K +    + +++D+ YK+ +  +LANG DGF ++ +  ++  D     +NV V  Y+  ++VI
Sbjct:   28 ELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKE-TPFLSNPGTNLVFEDEITALQPEVD-KLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKD-ELLRHDSGDQDINV-VSTYISKMKVI 538          
BLAST of 5'-nucleotidase protein vs. UniProt/SwissProt
Match: sp|Q05927|5NTD_BOVIN (5'-nucleotidase OS=Bos taurus OX=9913 GN=NT5E PE=1 SV=2)

HSP 1 Score: 107.842 bits (268), Expect = 7.292e-23
Identity = 121/519 (23.31%), Postives = 229/519 (44.12%), Query Frame = 2
Query:   71 ELIVLHFNDVYNVEGYPNEPQA----------GAARFCTALRDSRK-NCESLTLFSGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLM-QCVEKMNFQWLNSNVY--DSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDF--------------QVEF---TDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDII----------LTAVDADIALINSGTLRTD-GIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQ----------NELIRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVAVQNYLRAIQVI 1471
            EL +LH NDV++     +E  +          G AR  T +   R+     L L +GD    ++  T+ +G  +   +N    D    GNH+FD GV+ L+   ++++NF  L++N+     +  ++      YKI       +GI+G    +    L+    N V   +   +  E+  +L+    N IIAL H  +  D+++ + V  +D+V+GGH + F              Q  F   +DD +++ +     F           +    + +     + ++S   ++  +   +     +L+     ++G   V LD    S R +E N+GN ICD +          ++     + ++N G +R+     + G IT+ +L  +LP      ++ + G+ L +  E+ V +Y +  G F QVGGI   +  S+ PG RV    +K++           E +R+D+ YK+ +  +L +G DGF ++ +  +  D  +  + NV V  Y+  ++V+
Sbjct:   28 ELTILHTNDVHSRLEQTSEDSSKCVNASRCVGGVARLATKVHQIRRAEPHVLLLDAGDQYQGTIWFTVYKGTEVAHFMNALGYDAMALGNHEFDNGVEGLIDPLLKEVNFPILSANIKAKGPLASKISGLYSPYKILTVGDEVVGIVGYTSKE-TPFLSNPGTNLVFEDEITALQPEVD-KLKTLNVNKIIALGHSGFEVDKLIAQKVKGVDVVVGGHSNTFLYTGNPPSKEVPAGQYPFIVTSDDGRKVPVVQAYAFGKYLGYLKVEFDEKGNVVTSHGNPILLNSSIPEDPNIKADINKWRVKLDNYSTQELGKTIVYLDGTAQSCRFRECNMGNLICDAMINNNLRHPDEMSWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQATGEFLQVGGIHVVYDISRNPGDRV----VKLEVLCTQCRVPSYEPLRMDKVYKVILPSFLVSGGDGFQMIKDEKIKHDSGDQDI-NV-VSGYISKMKVL 538          
BLAST of 5'-nucleotidase protein vs. TrEMBL
Match: A0A4V3SGK1 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_002005 PE=3 SV=1)

HSP 1 Score: 539.265 bits (1388), Expect = 1.382e-179
Identity = 271/545 (49.72%), Postives = 375/545 (68.81%), Query Frame = 2
Query:   71 ELIVLHFNDVYNVEGYPNEPQAGAARFCTALRDSRKNCESLTLFSGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQCVEKMNFQWLNSNVYDSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEG---CNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVEFTDD-----KKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTAVDADIALINSGTLRTDGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQNELIRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVAVQNYLRAIQVINGFKCQKYRYRSSVVSMHQRKSIVMENSG-LDFQNLRLTPNRWGKVKSQITQTEKERC--TIGPKIEGRI 1672
            +L +LHFNDVYNVEG   EP AGAARF +AL+      +S+ LFSGD LSPSMIS+ITQG H+P ILN+  +  AV GNHD DFG+D L +C+E+ NF WLN+NV D  T+   A    Y I E  G++IGIIGL+E +WVATL+ VD  D+ V D CE       +L+  G   C+L+IALTHMRW NDR L   VP IDL+LGGHDHD+ VE+ D+     + R ++KSGTD+RN   I+++ +  +KT++ + VE++ VDS +E  +K+   V+  +  LE +M++ +G + V LDARFSSIR++ETN+GNFI DI+LT VDA++A+INSGTLR D II  G    RDL+NLLP++DP+VVL  TG Q+ E LENGVSQYPK EGRFP V G+ F F   +PPGSR+ ++S+ IQN+ + L+  +K+CVK Y+ANG+DG+++L ECPV++DDE+ P+L+  +QNY R IQV+ GF+  + R+R S+VS+ +R  +V E  G    Q  +   + W K +  + +  +      I P ++GRI
Sbjct:    8 QLNILHFNDVYNVEGQSTEPAAGAARFHSALKAHGAEDKSIVLFSGDALSPSMISSITQGRHVPPILNDLFVRCAVLGNHDLDFGLDILCECIEESNFPWLNTNVLDRDTNTPLAGLPTYHIIEHAGLRIGIIGLLEEEWVATLSCVDAEDLEVLDICEAARSWTQKLKQPGPDSCDLVIALTHMRWPNDRKLAAGVPEIDLILGGHDHDYVVEWLDEPGKPVRTRAILKSGTDYRNFSHIRLTFDKQAKTVSELTVEEVIVDSRWEINEKILCYVKTQTERLEKKMELSLGRVEVPLDARFSSIRTKETNVGNFISDIVLTGVDAEVAIINSGTLRADRIIEAGTFRLRDLINLLPMLDPMVVLQATGKQIYEALENGVSQYPKHEGRFPIVAGVRFAFDPREPPGSRIPLSSVTIQNKPLNLEAKHKLCVKHYMANGRDGYDMLRECPVLVDDEDGPILSTLIQNYFRTIQVLKGFEKARTRHRQSIVSIVERHHLVDELLGQTKSQGAKDVRHYWNKARHILIENHEMHSANAIAPTVDGRI 552          
BLAST of 5'-nucleotidase protein vs. TrEMBL
Match: A0A3R7CP79 (Trifunctional nucleotide phosphoesterase protein YfkN OS=Clonorchis sinensis OX=79923 GN=yfkN PE=3 SV=1)

HSP 1 Score: 538.11 bits (1385), Expect = 3.984e-179
Identity = 268/545 (49.17%), Postives = 374/545 (68.62%), Query Frame = 2
Query:   71 ELIVLHFNDVYNVEGYPNEPQAGAARFCTALRDSRKNCESLTLFSGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQCVEKMNFQWLNSNVYDSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEG---CNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVEFTDD-----KKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTAVDADIALINSGTLRTDGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQNELIRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVAVQNYLRAIQVINGFKCQKYRYRSSVVSMHQRKSIVMENSG-LDFQNLRLTPNRWGKVKSQITQTEKERC--TIGPKIEGRI 1672
            +L +LHFNDVYNVEG   EP AGAARF +AL+      +S+ LFSGD LSPSMIS+ITQG H+P ILN+  +  AV GNHD DFG+D L +C+E+ NF WLN+NV D  T+   A    Y I E  G++IGIIGL+E +WVATL+ VD  D+ V D CE       +L+  G   C+L+IALTHMRW NDR L   VP IDL+LGGHDHD+ +E+ D+     + R ++KSGTD+R+   I+++ +  +K ++ + VE++ VDS ++  +K+   V+  +  LE +M++ +G + V LDARFSSIR++ETN+GNFI DI+LT VDA++A+INSGTLR D II+ G    RDL+NLLP++DP+VVL  TG Q+ E LENGVSQYPK EGRFP V G+ F F   KPPGSR+ ++S+ IQN+ + L   +K+CVK Y+ANG+DG+++L ECPV++DDE+ P+L+  +QNY R IQV+ GF+  + R+R S+VS+ +R  +V E  G    Q  +   + W K +  + +  +      I P ++GRI
Sbjct:    8 QLNILHFNDVYNVEGQSTEPVAGAARFHSALKAHGAGDKSIVLFSGDALSPSMISSITQGRHVPPILNDLFVRCAVLGNHDLDFGLDILCECIEESNFPWLNTNVLDRDTNAPLAGLPTYHIIEHAGLRIGIIGLLEEEWVATLSCVDAEDLEVLDICEAARSWTQKLKQPGPDSCDLVIALTHMRWPNDRKLAAGVPEIDLILGGHDHDYVIEWLDEPGKPVRTRAILKSGTDYRSFSHIRLTFDKQAKIVSELTVEEVIVDSRWDTNEKILCYVKTQTERLEKKMELSLGRVEVPLDARFSSIRTKETNVGNFISDIVLTGVDAEVAIINSGTLRADRIIAAGTFRLRDLINLLPMLDPMVVLQATGKQIYEALENGVSQYPKHEGRFPIVAGVRFAFDPRKPPGSRIPLSSVTIQNKPLNLKAKHKLCVKHYMANGRDGYDMLRECPVLVDDEDGPILSTLIQNYFRTIQVLKGFEKARTRHRQSIVSIVERHHLVDELLGQTKSQGTKDVRHYWNKARHILIENHEMHSANAIAPMVDGRI 552          
BLAST of 5'-nucleotidase protein vs. TrEMBL
Match: A0A075A8K6 (Uncharacterized protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=T265_12878 PE=3 SV=1)

HSP 1 Score: 535.413 bits (1378), Expect = 1.029e-175
Identity = 267/545 (48.99%), Postives = 372/545 (68.26%), Query Frame = 2
Query:   71 ELIVLHFNDVYNVEGYPNEPQAGAARFCTALRDSRKNCESLTLFSGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQCVEKMNFQWLNSNVYDSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEG---CNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVEFTDD-----KKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTAVDADIALINSGTLRTDGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQNELIRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVAVQNYLRAIQVINGFKCQKYRYRSSVVSMHQRKSIVMENSG-LDFQNLRLTPNRWGKVKSQITQTEKERC--TIGPKIEGRI 1672
            +L +LHFNDVYNVEG   EP AGAARF +AL+      +S+ LFSGD LSPSMIS+ITQG H+P ILN+  +  AV GNHD DFG+D L +C+E+ NF WLN+NV D  T+   A    Y I E  G++IG+IGL+E +WVATL+ VD  D+ V D CE       +L+  G   C+L+IALTHMRW NDR L   VP IDL+LGGHDHD+  E+ D+     + R ++KSGTD+R+   I+++ +  +K ++ + VE++ VDS +E  +K+   V+  +  LE +M++ +G + V LDARFSSIR++ETN+GNF+ DI+LT VDA++A+INSGTLR D II  G    RDL+NLLP++DP+VVL  TG Q+ E LENGVSQYPK EGRFP V G+ F F   KPPGSR+ ++S+ IQN+ + L   +K+CVK Y+ANG+DG+++L ECPV++DDE+ P+L+  +QNY R IQV+ GF+  + R+R S+VS+ +R  +V E  G    Q  +   + W K +  + +  +      I P ++GRI
Sbjct:  171 QLHILHFNDVYNVEGQSTEPVAGAARFHSALKAHGAEDKSIVLFSGDALSPSMISSITQGRHVPPILNDLFVRCAVLGNHDLDFGLDILCECIEESNFPWLNTNVLDRDTNSPLAGLPTYHIIEHAGLRIGLIGLLEEEWVATLSCVDAEDLEVLDICEAARSWTQKLKQPGPESCDLVIALTHMRWPNDRKLAAGVPEIDLILGGHDHDYVTEWLDEPGKPVRTRAILKSGTDYRSFSHIRLTFDKQAKIVSELTVEEVIVDSRWEINEKILCYVKTQTERLEKKMELSLGRVEVPLDARFSSIRTKETNVGNFVSDIVLTGVDAEVAIINSGTLRADRIIEAGTFRLRDLINLLPMLDPMVVLQATGKQIYEALENGVSQYPKHEGRFPIVSGVRFAFDPRKPPGSRIPLSSVTIQNKPLNLKAKHKLCVKHYMANGRDGYDMLRECPVLVDDEDGPILSTLIQNYFRTIQVLKGFEKARTRHRQSIVSIVERHHLVDELLGQTKSQGTKDVRHYWNKARHILIENHEMHSANAIAPMVDGRI 715          
BLAST of 5'-nucleotidase protein vs. TrEMBL
Match: A0A1S8WII9 (5'-nucleotidase protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=X801_09962 PE=3 SV=1)

HSP 1 Score: 531.176 bits (1367), Expect = 1.739e-175
Identity = 262/522 (50.19%), Postives = 362/522 (69.35%), Query Frame = 2
Query:   71 ELIVLHFNDVYNVEGYPNEPQAGAARFCTALRDSRKNCESLTLFSGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQCVEKMNFQWLNSNVYDSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEG---CNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVEFTDD-----KKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTAVDADIALINSGTLRTDGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQNELIRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVAVQNYLRAIQVINGFKCQKYRYRSSVVSMHQRKSIVMENSG-LDFQNLRLTPNRWGKVK 1609
            +L +LHFNDVYNVEG   EP AGAARF +AL+      +S+ LFSGD LSPSMIS+ITQG H+P ILN+  +  AV GNHD DFG+D L +C+E+ NF WLN+NV D  T+   A    Y I E  G++IG+IGL+E +WVATL+ VD  D+ V D CE       +L+  G   C+L+IALTHMRW NDR L   VP IDL+LGGHDHD+  E+ D+     + R ++KSGTD+R+   I+++ +  +K ++ + VE++ VDS +E  +K+   V+  +  LE +M++ +G + V LDARFSSIR++ETN+GNF+ DI+LT VDA++A+INSGTLR D II  G    RDL+NLLP++DP+VVL  TG Q+ E LENGVSQYPK EGRFP V G+ F F   KPPGSR+ ++S+ IQN+ + L   +K+CVK Y+ANG+DG+++L ECPV++DDE+ P+L+  +QNY R IQV+ GF+  + R+R S+VS+ +R  +V E  G    Q  +   + W K +
Sbjct:   89 QLHILHFNDVYNVEGQSTEPVAGAARFHSALKAHGAEDKSIVLFSGDALSPSMISSITQGRHVPPILNDLFVRCAVLGNHDLDFGLDILCECIEESNFPWLNTNVLDRDTNSPLAGLPTYHIIEHAGLRIGLIGLLEEEWVATLSCVDAEDLEVLDICEAARSWTQKLKQPGPESCDLVIALTHMRWPNDRKLAAGVPEIDLILGGHDHDYVTEWLDEPGKPVRTRAILKSGTDYRSFSHIRLTFDKQAKIVSELTVEEVIVDSRWEINEKILCYVKTQTERLEKKMELSLGRVEVPLDARFSSIRTKETNVGNFVSDIVLTGVDAEVAIINSGTLRADRIIEAGTFRLRDLINLLPMLDPMVVLQATGKQIYEALENGVSQYPKHEGRFPIVSGVRFAFDPRKPPGSRIPLSSVTIQNKPLNLKAKHKLCVKHYMANGRDGYDMLRECPVLVDDEDGPILSTLIQNYFRTIQVLKGFEKARTRHRQSIVSIVERHHLVDELLGQTKSQGTKDVRHYWNKAR 610          
BLAST of 5'-nucleotidase protein vs. TrEMBL
Match: A0A5J4NHH2 (5'-nucleotidase OS=Paragonimus westermani OX=34504 GN=DEA37_0008181 PE=3 SV=1)

HSP 1 Score: 518.85 bits (1335), Expect = 1.210e-171
Identity = 262/561 (46.70%), Postives = 376/561 (67.02%), Query Frame = 2
Query:   56 NTMTSELIVLHFNDVYNVEGYPNEPQAGAARFCTALRDSRKNCESLTLFSGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQCVEKMNFQWLNSNVYDSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEG------CNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVEFTDDK---------KRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTAVDADIALINSGTLRTDGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQNELIRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVAVQNYLRAIQVINGFKCQKYRYRSSVVSMHQRKSIVMENSG----LDFQNLRLTPNRWGKVKSQITQTEKERCT---IGPKIEGRI 1672
            N     L +LHFNDVYNVEG   EP  GAARF TAL++      ++ LFSGD LSPSM+ST+TQG HMP +LN   +D AV GNHD DFG+D + +C+EK  F WLN+NV+D  T++  +    + I E  G+ IG+IGL+E +W+ TL+ +D   + V D CE+G   A +L+  G      C+L+IALTHMRW NDR L   VP IDL+LGGHDH ++VE+  +           R +IKSGTDF N   I+I  +   + I+ +++EK+ VDS +++++++   V+  + +L+ ++ + +G I V LDARFSS+RSQETN+GNF+CDIILT +DA +A++NSGTLR D +I  GD   RDL NLLP++D +V+L  TG Q+++ LENGVSQYPKLEGRFP V GI F F   + PG+R+  +S+  Q++ + L+++Y++ VK Y+A+GKDG++VL ECPV++DDE  P+L+  +QNY RAIQV+ GF+  + R+R S+VS+ ++  + ++NSG    L+    R T   W K   Q+   EKE  +   I P ++GRI
Sbjct:    6 NPHIVSLDILHFNDVYNVEGRTTEPVGGAARFYTALKEHGAGETAVVLFSGDALSPSMLSTVTQGRHMPTVLNALLVDCAVLGNHDLDFGLDLMYECIEKSRFPWLNTNVFDMDTNKPLSGAPDHHIVEHGGLTIGLIGLLEEEWITTLSCIDHTALDVHDMCEVGRRWARELKEPGPNGRAACDLVIALTHMRWPNDRKLSAEVPEIDLILGGHDHKYEVEWPGEADRQIVPKKNTRAIIKSGTDFENFSHIRIKFDKKQRRISELKIEKVVVDSHWKEDEEMKSYVEKLTEKLDKKLDMILGRIEVPLDARFSSVRSQETNVGNFVCDIILTGLDAHVAIVNSGTLRADRLIPAGDFCLRDLTNLLPMLDSMVLLQATGEQILKALENGVSQYPKLEGRFPLVAGIRFSFDPRRSPGNRIPCSSVTTQDKPLELNKTYRLAVKHYMASGKDGYDVLKECPVLVDDEEGPILSTLIQNYFRAIQVLKGFEHSRTRHRQSIVSIAEKHRL-LQNSGYPEILNPDEKRQT--YWQKA-YQLLIEEKEAQSANAIAPGLDGRI 562          
BLAST of 5'-nucleotidase protein vs. Ensembl Cavefish
Match: nt5e (5'-nucleotidase ecto [Source:NCBI gene;Acc:103031009])

HSP 1 Score: 135.961 bits (341), Expect = 6.141e-33
Identity = 136/541 (25.14%), Postives = 242/541 (44.73%), Query Frame = 2
Query:   71 ELIVLHFNDVY-NVE------GYPNEPQ--AGAARFCTALRDSR-KNCESLTLFSGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQ-CVEKMNFQWLNSNVY--DSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDF--------------QVEF---TDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTAV----------DADIALINSGTLRT--DGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKI------QNELIRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVA-VQNYLRAIQVINGFKCQKYRYRSSVVSMHQRKSIVM 1546
            EL +LH NDV+  VE      G   +P   AG AR  T +R+ R +   SL L +GD    ++   + +G      +N+   D    GNH+FD GVD L +  ++ +N   L++N+    ++   +    + +KIF     K+GI+G    +  A L+   P+ +   +   +  E+  +L   G N IIAL H  +A D+ + K V  +D+V+GGH + F                 F   +DD +++ +     F           +++  +   E   + +DS  E++  +   V++    L       +G   V L+  F   R +E N+GN ICD ++                  ++N G +R+  D     G IT  D++ +LP      ++ + G+ L++  E+ V ++    G F QV G +  +  +KP G RV   SI        + E +   + YK+ +  YL +G DG++++ E  +  D  +   L++A V  Y+   + ++     + R+ SS  S  + +S VM
Sbjct:   28 ELALLHTNDVHARVEETNKDSGKCTKPPCFAGVARRFTKIRELRSRETNSLLLDAGDQFQGTVWFNLYKGAEAAHFMNKLGYDAMALGNHEFDNGVDGLTKPFLQDVNCTVLSANIQPDSTLAPTISGSYLAFKIFNFGSEKVGIVGYTSKETPA-LSLPGPHLIFEDEVTALQREVN-KLTTLGVNKIIALGHSGFAVDQEIAKKVRGVDVVIGGHTNTFLYTGSPPSTEVPAGSYPFMVKSDDGRQVPVVQAYAFGKYLGYLKVTFDSAGNVKKAEGNPILLDSSIEEDATIKADVESWKVNLANYSSQYVGRTLVYLNGSFEECRFRECNLGNLICDAMVHHYIKYPDEVQWNHVGSCILNGGGIRSPIDERNRNGTITMEDVIAVLPFGGTFDLVQLNGSTLLQAFEHSVRRHGGSTGEFLQVSGFQIEYDLTKPAGERVKNASILCTECRVPRYEPLDPKKVYKVVMPSYLVDGGDGYSMIKEKKLKHDSGD---LDIAVVAGYITERKRVHPAVEGRIRFYSSFNSAIRLQSPVM 563          
BLAST of 5'-nucleotidase protein vs. Ensembl Nematostella
Match: EDO42016 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2K3])

HSP 1 Score: 98.9821 bits (245), Expect = 2.171e-21
Identity = 118/502 (23.51%), Postives = 206/502 (41.04%), Query Frame = 2
Query:   71 ELIVLHFNDVY------NVEGYPNEPQ--------AGAARFCTALRDSRKNCESLTLFS-GDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQCVEKMNFQWLNSNVYDSMTDELFADG----IKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQ-----LRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVEFTDDK----------------------KRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICD-IILTAVD----------ADIALINSGTL-RTDGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQNELIRLDQS------YKICVKQYLANGKDGFNVL 1384
            +L VLH ND +      N  G   + Q         G AR  T ++  R    ++ L S GD  + ++     +G+    ++NE   D    GNHDFD G     + +  +    + SNV  ++   L  +G    ++  I    G +IG+ G +  D    +++  P   +VK   E+    A+Q     L+ +G N II L H     D+ + + V  +DL++GGH H F   ++ DK                      K++ I     F           ++   + S     + +D    K+  +   VQ     ++    IKIG   V L+         E N+GN I D +++T  +          A + L  +G + ++  +  +G +TY +L + +P  +   VL++ G+ L  VLE  V  +P     F QV G++  +  S+ P +RV    ++    L+ +         YK+    ++A G  GF+V 
Sbjct:   25 KLTVLHTNDFHSRFEETNPYGTVCKAQDLAKDGCYGGVARRATEIKRIRAKENNVILLSAGDVFTGTLWYKEYRGNATWSVMNEMGYDAMTLGNHDFDDGPAITARFLRNIKCPVVVSNVNCTLEPALMDNGRPLILQSTILTVGGKRIGLAGYVTHD-TPRISSPGPGQ-LVKFAPEVA---AVQSAVDDLKRQGVNKIIVLGHAEMEVDKAVARMVDGVDLLVGGHTHTFL--YSGDKPPAQDVPEGPYPLVVTSEATPGKKVPIVHAYKFGKYLGRLDVEFDDKGDLTSWTGNPVLLDRSVAKDSALEQQVQRMKGRVDKLASIKIGRSLVQLNGDRDVCWRGECNLGNVITDAMVMTYANKTATSMQWTGASVGLQTAGNIFKSIELTPEGYVTYGELFDAMPYSNTFDVLNMKGSDLRGVLEQSVRTWPSYT--FLQVSGLKVSYDMSREPMTRVIKVKVRCAQCLVPVYHDLNDTGIYKVITNSWIAGGGSGFSVF 517          
BLAST of 5'-nucleotidase protein vs. Ensembl Medaka
Match: NT5E (5'-nucleotidase [Source:NCBI gene;Acc:101168262])

HSP 1 Score: 120.553 bits (301), Expect = 7.126e-28
Identity = 129/512 (25.20%), Postives = 215/512 (41.99%), Query Frame = 2
Query:   17 RNCTNISL*STRINTMTSELIVLHFNDVY-NVE---------GYPNEPQAGAARFCTALRDSRKNCESLTLF-SGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQ-CVEKMNFQWLNSNV-YDSMTDELFADG-IKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAI-QLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVEFTDDKKRLLIKSGT-DFRXXXXXXXXXXXXXXXXXXXEVEKLTV------------------DSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIIL------------TAVDADIALINSGTLRT--DGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQN------ELIRLDQSYKICVKQYLANGKDGFNVLAE 1390
            R C  + + S    T   +L++LH NDV+  +E         G       G AR  TA++  R +  ++ L  +GD    ++     +G      +N    D   +GNH+FD  V+ LM+  +E +    L++N+  D+     F    + +KIF     K+G++G    +   TL+   P      +   +  +L + +L+  G N IIAL H  +  D+ + K V  +D+V+GGH + F   +T       +++G   F         +           + KL V                  DS  +++  V   V+     L       +G   V L+    + R QE N+GN ICD ++              V A I   N G +RT  D     G IT  DL+++LP      ++ ++G+ L ++ E  V +Y +  G F QV G    +  SK PG+RV    I   +      E ++    YK+ +  YL  G DGF+++ E
Sbjct:   15 RFCPVLLILSGPCMTAAWDLVLLHTNDVHARIEETSVHSGKCGATGGCYGGVARRATAIQSIRGSETNVLLLDAGDQFQGTVWFNYYKGAEAAHFMNLLRYDAMAFGNHEFDNEVEGLMKPFLEDIKCTILSANIKTDTTLASTFGTTYVPFKIFTFGTEKVGVVGYTSKE-TPTLSRPGPRLRFEDEVSAL--QLQVDKLQTLGINKIIALGHSGFTVDKEIAKRVRGVDVVIGGHTNTFL--YTGTPPSTEVRAGPYPFMVTSLDGRQVPVVQAYAYGKYLGKLKVTFDENGNVIRSSGNPILLDSSIQQDPVVLSDVEEWKKSLANYSSQVVGKTMVFLNGSAEACRFQECNLGNLICDAMVENNIRFSGGNQWNHVSASI--FNGGGIRTSIDEQTRNGSITMEDLISVLPFGGTFDLVQLSGSTLKKIFEYSVRRYGQRAGEFLQVSGFHIEYDPSKEPGNRVRSLEILCTHCRVPMYEPVQDSMDYKVVMPSYLVTGGDGFSMIPE 519          
BLAST of 5'-nucleotidase protein vs. Ensembl Medaka
Match: NT5E (5'-nucleotidase [Source:NCBI gene;Acc:101174549])

HSP 1 Score: 113.235 bits (282), Expect = 1.324e-25
Identity = 112/489 (22.90%), Postives = 205/489 (41.92%), Query Frame = 2
Query:   71 ELIVLHFNDVY-NVEGYPNEPQ---------AGAARFCTALRD-SRKNCESLTLFSGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQ-CVEKMNFQWLNSNV--YDSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVE-----------------FTDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDII----------LTAVDADIALINSGTLRT--DGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQN------ELIRLDQSYKICVKQYLANGKDGFNVLAE 1390
            EL +LH NDV+  +E    +           AG AR  T + +  RK    L L +GD    ++     +G      +N+   D+  +GNH+FD GVD L Q  ++++NF  + + +    S+   +      + +F     K+ ++G    +    L+   P  +  +D  E   +   QL   G N IIAL H  +  D+ + K V  ID+V+GGH + F                     ++D + + +     F           + +  +       + ++S   ++  +   V+     LE      IG   V L+  F   R +E N+GN ICD +          L      + ++NSG++R   D   + G IT  +++++LP      ++ + G+ L +  E+ V +Y  + G F QV GI   +  SKP   RV    ++         E +   ++Y + +  ++  G DG++++ +
Sbjct:   30 ELTLLHTNDVHARIEETSEDSSKCHEAGSCFAGVARMFTKVTEIRRKETHVLFLDAGDQFQGTVWFNYYKGKEAAHFMNKLGYDVMTFGNHEFDNGVDSLTQNFLQRVNFSVVCATIKPLHSLVANMSRFYRPFAVFNVGSEKVAVVGYTTKE-TPVLSAPGPY-LKFEDEVEALQDQVNQLEKLGVNKIIALGHSGFEVDKDIAKRVRGIDVVIGGHTNTFLYTGKPPSTEVPRGPYPFNVSSNDGRWVPVVQAFAFGKYLGYLKVTFDQAGKVVKAVGNPILMNSSIPQDPGILSDVEKWKKGLEQYSSQYIGQTLVYLNGTFEECRFRECNLGNLICDAMIYNYIRYSNKLQWNHVGVCMLNSGSIRATIDERSTNGSITMEEILSVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGGMSGEFLQVSGIRVEYDLSKPVNQRVVSLLMRCTECRVPKFEPLDPQKTYTVVMTSFMVGGGDGYSMIQD 516          
BLAST of 5'-nucleotidase protein vs. Ensembl Medaka
Match: NT5E (5'-nucleotidase [Source:NCBI gene;Acc:101174549])

HSP 1 Score: 113.235 bits (282), Expect = 1.324e-25
Identity = 112/489 (22.90%), Postives = 205/489 (41.92%), Query Frame = 2
Query:   71 ELIVLHFNDVY-NVEGYPNEPQ---------AGAARFCTALRD-SRKNCESLTLFSGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQ-CVEKMNFQWLNSNV--YDSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVE-----------------FTDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDII----------LTAVDADIALINSGTLRT--DGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQN------ELIRLDQSYKICVKQYLANGKDGFNVLAE 1390
            EL +LH NDV+  +E    +           AG AR  T + +  RK    L L +GD    ++     +G      +N+   D+  +GNH+FD GVD L Q  ++++NF  + + +    S+   +      + +F     K+ ++G    +    L+   P  +  +D  E   +   QL   G N IIAL H  +  D+ + K V  ID+V+GGH + F                     ++D + + +     F           + +  +       + ++S   ++  +   V+     LE      IG   V L+  F   R +E N+GN ICD +          L      + ++NSG++R   D   + G IT  +++++LP      ++ + G+ L +  E+ V +Y  + G F QV GI   +  SKP   RV    ++         E +   ++Y + +  ++  G DG++++ +
Sbjct:   30 ELTLLHTNDVHARIEETSEDSSKCHEAGSCFAGVARMFTKVTEIRRKETHVLFLDAGDQFQGTVWFNYYKGKEAAHFMNKLGYDVMTFGNHEFDNGVDSLTQNFLQRVNFSVVCATIKPLHSLVANMSRFYRPFAVFNVGSEKVAVVGYTTKE-TPVLSAPGPY-LKFEDEVEALQDQVNQLEKLGVNKIIALGHSGFEVDKDIAKRVRGIDVVIGGHTNTFLYTGKPPSTEVPRGPYPFNVSSNDGRWVPVVQAFAFGKYLGYLKVTFDQAGKVVKAVGNPILMNSSIPQDPGILSDVEKWKKGLEQYSSQYIGQTLVYLNGTFEECRFRECNLGNLICDAMIYNYIRYSNKLQWNHVGVCMLNSGSIRATIDERSTNGSITMEEILSVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGGMSGEFLQVSGIRVEYDLSKPVNQRVVSLLMRCTECRVPKFEPLDPQKTYTVVMTSFMVGGGDGYSMIQD 516          
BLAST of 5'-nucleotidase protein vs. Planmine SMEST
Match: SMESG000032250.1 (SMESG000032250.1)

HSP 1 Score: 1069.3 bits (2764), Expect = 0.000e+0
Identity = 541/541 (100.00%), Postives = 541/541 (100.00%), Query Frame = 2
Query:   62 MTSELIVLHFNDVYNVEGYPNEPQAGAARFCTALRDSRKNCESLTLFSGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQCVEKMNFQWLNSNVYDSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVEFTDDKKRLLIKSGTDFRXXXXXXXXXXXXXXXXXXXEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTAVDADIALINSGTLRTDGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQNELIRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVAVQNYLRAIQVINGFKCQKYRYRSSVVSMHQRKSIVMENSGLDFQNLRLTPNRWGKVKSQITQTEKERCTIGPKIEGRIVRID 1684
            MTSELIVLHFNDVYNVEGYPNEPQAGAARFCTALRDSRKNCESLTLFSGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQCVEKMNFQWLNSNVYDSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVEFTDDKKRLLIKSGTDFRNIGKIKISINNNSKTINSIEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTAVDADIALINSGTLRTDGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQNELIRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVAVQNYLRAIQVINGFKCQKYRYRSSVVSMHQRKSIVMENSGLDFQNLRLTPNRWGKVKSQITQTEKERCTIGPKIEGRIVRID
Sbjct:    1 MTSELIVLHFNDVYNVEGYPNEPQAGAARFCTALRDSRKNCESLTLFSGDCLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQCVEKMNFQWLNSNVYDSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATVDPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSIDLVLGGHDHDFQVEFTDDKKRLLIKSGTDFRNIGKIKISINNNSKTINSIEVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSIRSQETNIGNFICDIILTAVDADIALINSGTLRTDGIISKGDITYRDLMNLLPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKPPGSRVDVNSIKIQNELIRLDQSYKICVKQYLANGKDGFNVLAECPVIMDDENAPVLNVAVQNYLRAIQVINGFKCQKYRYRSSVVSMHQRKSIVMENSGLDFQNLRLTPNRWGKVKSQITQTEKERCTIGPKIEGRIVRID 541          
The following BLAST results are available for this feature:
BLAST of 5'-nucleotidase protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
NT5E5.628e-2423.695'-nucleotidase ecto [Source:HGNC Symbol;Acc:HGNC:... [more]
NT5E1.868e-821.825'-nucleotidase ecto [Source:HGNC Symbol;Acc:HGNC:... [more]
NT5E2.106e-628.575'-nucleotidase ecto [Source:HGNC Symbol;Acc:HGNC:... [more]
NT5E3.638e-624.775'-nucleotidase ecto [Source:HGNC Symbol;Acc:HGNC:... [more]
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BLAST of 5'-nucleotidase protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 5'-nucleotidase protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG118831.819e-13640.72gene:FBgn0033538 transcript:FBtr0088283[more]
CG118833.324e-13640.72gene:FBgn0033538 transcript:FBtr0088284[more]
CG118831.669e-13540.72gene:FBgn0033538 transcript:FBtr0339461[more]
CG118831.669e-13540.72gene:FBgn0033538 transcript:FBtr0113063[more]
CG301033.782e-2924.59gene:FBgn0050103 transcript:FBtr0086929[more]
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BLAST of 5'-nucleotidase protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nt5e1.970e-2925.565'-nucleotidase, ecto (CD73) [Source:ZFIN;Acc:ZDB-... [more]
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BLAST of 5'-nucleotidase protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
NT5E2.387e-2925.505'-nucleotidase, ecto (CD73) [Source:NCBI gene;Acc... [more]
NT5E3.826e-2925.145'-nucleotidase, ecto (CD73) [Source:NCBI gene;Acc... [more]
NT5E3.901e-2925.245'-nucleotidase, ecto (CD73) [Source:NCBI gene;Acc... [more]
NT5E4.240e-2925.245'-nucleotidase, ecto (CD73) [Source:NCBI gene;Acc... [more]
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BLAST of 5'-nucleotidase protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Nt5el5.291e-13142.955' nucleotidase, ecto-like [Source:MGI Symbol;Acc:... [more]
Nt5e7.841e-2222.955' nucleotidase, ecto [Source:MGI Symbol;Acc:MGI:9... [more]
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BLAST of 5'-nucleotidase protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O34313|NTPES_BACSU3.379e-3627.78Trifunctional nucleotide phosphoesterase protein Y... [more]
sp|A9BJC1|MGGB_PETMO2.153e-2726.48Mannosylglucosyl-3-phosphoglycerate phosphatase OS... [more]
sp|Q9XZ43|5NTD_LUTLO5.625e-2723.72Protein 5NUC OS=Lutzomyia longipalpis OX=7200 GN=5... [more]
sp|P21589|5NTD_HUMAN2.703e-2323.695'-nucleotidase OS=Homo sapiens OX=9606 GN=NT5E PE... [more]
sp|Q05927|5NTD_BOVIN7.292e-2323.315'-nucleotidase OS=Bos taurus OX=9913 GN=NT5E PE=1... [more]
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BLAST of 5'-nucleotidase protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4V3SGK11.382e-17949.72Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A3R7CP793.984e-17949.17Trifunctional nucleotide phosphoesterase protein Y... [more]
A0A075A8K61.029e-17548.99Uncharacterized protein (Fragment) OS=Opisthorchis... [more]
A0A1S8WII91.739e-17550.195'-nucleotidase protein (Fragment) OS=Opisthorchis... [more]
A0A5J4NHH21.210e-17146.705'-nucleotidase OS=Paragonimus westermani OX=34504... [more]
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BLAST of 5'-nucleotidase protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nt5e6.141e-3325.145'-nucleotidase ecto [Source:NCBI gene;Acc:1030310... [more]
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BLAST of 5'-nucleotidase protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 5'-nucleotidase protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 5'-nucleotidase protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO420162.171e-2123.51Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of 5'-nucleotidase protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
NT5E7.126e-2825.205'-nucleotidase [Source:NCBI gene;Acc:101168262][more]
NT5E1.324e-2522.905'-nucleotidase [Source:NCBI gene;Acc:101174549][more]
NT5E1.324e-2522.905'-nucleotidase [Source:NCBI gene;Acc:101174549][more]
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BLAST of 5'-nucleotidase protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000032250.10.000e+0100.00SMESG000032250.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016363 ID=SMED30016363|Name=5'-nucleotidase protein|organism=Schmidtea mediterranea sexual|type=transcript|length=2652bp
CTCTATTATTCAATATAGAAATTGTACTAATATTTCTTTATGATCGACTC
GGATTAATACAATGACTTCAGAATTAATCGTATTGCATTTCAATGATGTT
TACAACGTCGAAGGTTACCCAAATGAACCTCAAGCTGGAGCAGCTCGATT
TTGTACTGCACTTCGGGATTCTCGAAAGAATTGCGAGTCTTTGACTTTGT
TTTCTGGAGACTGTCTGAGTCCATCGATGATCAGCACGATAACCCAAGGA
GATCATATGCCAGAGATCTTGAATGAATTCGATATCGACTTGGCTGTGTA
TGGCAATCATGATTTTGATTTCGGCGTTGATAAACTGATGCAATGTGTGG
AGAAAATGAATTTCCAGTGGTTAAATAGCAATGTTTACGACAGTATGACT
GACGAGTTATTCGCTGATGGGATAAAGTACAAAATTTTCGAAAGATGTGG
AGTAAAAATCGGAATAATTGGTTTGATTGAACCGGATTGGGTAGCCACTT
TGGCTACTGTTGATCCGAATGACGTCATCGTGAAGGATTTCTGTGAAATC
GGTAACGAGCTTGCGATCCAATTGAGAAATGAAGGTTGCAACCTTATCAT
TGCTCTCACTCATATGAGATGGGCCAACGATCGGATTCTTTTTAAAAATG
TTCCGTCAATCGACTTGGTACTCGGTGGGCATGACCATGATTTTCAAGTC
GAATTCACGGATGATAAAAAGCGTTTACTCATCAAAAGTGGAACCGATTT
TCGGAATATTGGAAAAATAAAAATTTCAATTAATAACAACAGCAAAACAA
TAAATTCGATAGAAGTGGAAAAATTGACCGTTGATAGCGGTTATGAAAAA
GAGAAAAAAGTCAACGATTTAGTTCAGAATGCTTCTTCGGAACTGGAAGG
ACGAATGAAAATTAAAATCGGAACAATTTCTGTTGACTTGGACGCCAGAT
TTTCTAGCATTCGATCACAGGAAACGAATATTGGGAATTTCATTTGTGAT
ATTATTTTAACGGCAGTCGATGCCGACATTGCTTTGATTAATTCTGGGAC
TTTGAGAACCGATGGAATTATATCGAAAGGAGATATTACTTATCGAGATC
TTATGAACTTATTGCCGGTCATTGACCCTTTAGTTGTGCTTTCAATAACT
GGGGCCCAGTTAGTAGAAGTTCTAGAAAACGGAGTTTCTCAATATCCAAA
ATTAGAAGGTCGCTTTCCTCAAGTGGGTGGCATAGAATTTCGATTTTGGT
CTTCAAAACCTCCAGGAAGTCGCGTTGATGTGAATTCTATCAAAATTCAA
AACGAATTAATTCGGTTAGATCAAAGCTATAAAATATGCGTAAAGCAATA
TTTGGCAAATGGTAAAGACGGATTCAATGTTTTGGCAGAATGTCCAGTAA
TAATGGACGATGAAAATGCCCCAGTATTAAACGTTGCCGTTCAAAACTAT
CTTAGGGCTATTCAGGTGATAAACGGTTTTAAATGCCAAAAATACAGATA
TCGGTCCAGTGTGGTCAGCATGCATCAGAGAAAATCTATTGTCATGGAGA
ATTCGGGATTGGATTTTCAAAATCTGAGGTTAACTCCAAATAGATGGGGT
AAAGTGAAAAGTCAAATTACACAAACAGAAAAAGAAAGATGTACCATTGG
TCCTAAAATAGAAGGCAGAATAGTCAGAATAGATTGAAATATTATTTTCC
TAATGATTGAAATATTAGTGATTCAATAAAATTGTTCTCCTTCCTAATTT
TATAAATGTAGAACGAAATTTATTGTATTAATAATAATAATTACATCAAG
AATTTTTATTATTATTTGGCAATGAATCAGTAATGATTCGTTGTTTTATT
GATATCAGTAGGTAGTAATGTGCTTTCCAGATACTTGATTTTTCTGAAAA
ATGTAAGAACCCTCCTTGAATTTTTCCGAAGTCCATGTTTTGCTCGGTAC
AGCTCCCATTTTTTTTGTTTAAATCGATGATTAGGTCATTTAAAAAATAA
TTTAAATCACGATCACATCACTATTACGTAACCTTTCCATGATATTTTTA
CATATAAATGATCGTCGTGTTGTACCCTACCATTTCATACATTCGAATTT
TGTTTAAATCAATTATTACATAACTTCTAAATCCTTCACAAAACCATTGA
TTATGTCACCAATTTTAACATTCATCTAGAATTATTGATCCCTATAGACG
TAATTTTTGACGCTTTTTCTACTGGTACTATTACTTAATCGACAATTTGA
ATATTTAAAAAACTTAATGTAAAAAACATAAAAAACAAGACTTGTTAATA
TTAATACATTTATTCGCGTATTAAAAACCTAGAGAAAGCCGAGCACTGAC
TAATTTCCAAATTGTCGATATCACATACTTTTCATCCATTTTATTGCCTT
GTGTTTATCATCTGGACAAACCAACTTTACCCATCTGTATATTCAATTTC
TTCAAAAGCATCAATAAAATTGATGAATTTTCATTGCAACTTAGATTCTT
GTTGATTTTCATTAACACCTTATTTACTGCAACAACATCTTGTTTTGATA
ATTGATAATATGTTGAAATTTATTTATTCTAAATAATTCTTTAAATTATA
ATTAATAAGTGTTATTTGTCTTATTTTAGCCAAAGGAGAAGTTTTGGATT
CT
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protein sequence of SMED30016363-orf-1

>SMED30016363-orf-1 ID=SMED30016363-orf-1|Name=SMED30016363-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=542bp
MTSELIVLHFNDVYNVEGYPNEPQAGAARFCTALRDSRKNCESLTLFSGD
CLSPSMISTITQGDHMPEILNEFDIDLAVYGNHDFDFGVDKLMQCVEKMN
FQWLNSNVYDSMTDELFADGIKYKIFERCGVKIGIIGLIEPDWVATLATV
DPNDVIVKDFCEIGNELAIQLRNEGCNLIIALTHMRWANDRILFKNVPSI
DLVLGGHDHDFQVEFTDDKKRLLIKSGTDFRNIGKIKISINNNSKTINSI
EVEKLTVDSGYEKEKKVNDLVQNASSELEGRMKIKIGTISVDLDARFSSI
RSQETNIGNFICDIILTAVDADIALINSGTLRTDGIISKGDITYRDLMNL
LPVIDPLVVLSITGAQLVEVLENGVSQYPKLEGRFPQVGGIEFRFWSSKP
PGSRVDVNSIKIQNELIRLDQSYKICVKQYLANGKDGFNVLAECPVIMDD
ENAPVLNVAVQNYLRAIQVINGFKCQKYRYRSSVVSMHQRKSIVMENSGL
DFQNLRLTPNRWGKVKSQITQTEKERCTIGPKIEGRIVRID*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR0369075'-Nucleotdase_C_sf
IPR0061795_nucleotidase/apyrase
IPR029052Metallo-depent_PP-like
IPR0083345'-Nucleotdase_C
IPR004843Calcineurin-like_PHP_ApaH
Vocabulary: molecular function
TermDefinition
GO:0016787hydrolase activity
GO:0000166nucleotide binding
Vocabulary: biological process
TermDefinition
GO:0009166nucleotide catabolic process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0061795'-Nucleotidase/apyrasePRINTSPR01607APYRASEFAMLYcoord: 193..216
score: 25.74
coord: 3..21
score: 23.53
coord: 341..364
score: 28.35
coord: 174..191
score: 33.88
coord: 416..435
score: 24.02
IPR0061795'-Nucleotidase/apyrasePANTHERPTHR115755'-NUCLEOTIDASE-RELATEDcoord: 7..540
IPR004843Calcineurin-like phosphoesterase domain, ApaH typePFAMPF00149Metallophoscoord: 7..210
e-value: 1.6E-5
score: 25.5
IPR029052Metallo-dependent phosphatase-likeGENE3DG3DSA:3.60.21.10coord: 3..282
e-value: 2.7E-59
score: 202.6
IPR0083345'-Nucleotidase, C-terminalPFAMPF028725_nucleotid_Ccoord: 285..442
e-value: 1.7E-41
score: 142.1
IPR0369075'-Nucleotidase, C-terminal domain superfamilyGENE3DG3DSA:3.90.780.10coord: 285..479
e-value: 4.0E-50
score: 172.4
IPR0369075'-Nucleotidase, C-terminal domain superfamilySUPERFAMILYSSF558165'-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domaincoord: 284..471
NoneNo IPR availablePANTHERPTHR11575:SF24RIKEN CDNA 4933425L06coord: 7..540
NoneNo IPR availableSUPERFAMILYSSF56300Metallo-dependent phosphatasescoord: 4..279