SMED30016357

Overview
NameSMED30016357
Smed IDSMED30016357
Length (bp)4095
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30016357 (SMED30016357) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30016357 (SMED30016357) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30016357 (SMED30016357) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30016357 (SMED30016357) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30016357

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30016357SMESG000061508.1 SMESG000027427.1 dd_Smed_v4_5483_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30016357SMESG000061508.1 SMESG000027427.1 dd_Smed_v4_5483_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30016357SMESG000061508.1 SMESG000027427.1 dd_Smed_v4_5483_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30016357SMESG000061508.1 SMESG000027427.1 dd_Smed_v4_5483_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30016357 vs. Planmine SMEST
Match: SMESG000047715.1 (SMESG000047715.1)

HSP 1 Score: 839.721 bits (2168), Expect = 0.000e+0
Identity = 405/408 (99.26%), Postives = 405/408 (99.26%), Query Frame = 1 
Query: 1435 FIDSKNMNPNNFPYDSHNFMLDNCKSSCNNFPVQYGNRFVESVCPNNQYYHNFTNNSYMHSPYIIYDPRTNSFQNVLLVPQMMPMINHSIDMNEENQFSLQRLHNNGRNIQNCPYPDYKDYPVQESFPLNDQYQNLENQVFSETNGFSYNNLGGYISHDFNEGEEDVNEIHQTELESLHSVNRNISGDSEDYTSEDDQLNTVHGDEMLEELQKLSSVFTNVVNVSIMSRNSNISEPVELLNINNSSRFKKTRIKERTTYWNNKQAIVKDNLVKIIPSEILKIPFIDNETLNRFENEIFGDQLAEKDSEEKIFEQPDCIHSTLHCPGGFNDLRIHKHSNRTSQRCRSTATPRSLNEFPHMSNQIHNQNISSRYASHRKSLQIPIPFPLLVNKFIPLLSPVIPHCKKAFF 2658
            FIDSKNMNPNNFPYDSHNFMLDNCKSSCNNFPVQYGNRFVESVCPNNQYYHNFTNNSYMHSPYIIYDPRTNSFQNVLLVPQMMPMINHSIDMNEENQFSLQRLHNNGRNIQNCPYPDYKDYPVQESFPLNDQYQNLENQVFSETNGFSYNNLGGYISHDFNEGEEDVNEIHQTELESLHSVNRNISGDSEDYTSEDDQLNTVHGDEMLEELQKLSSVFTNVVNVSIMSRNSNISEPVELLNINNSSRFKKTRIKERTTYWNNKQAIVKDNLVKIIPSEILKIPFIDNETLNRFENEIFGDQLAEK  EEKIFEQPDCIHSTLHCPGGFNDLRIHKHSNRTSQRCRSTATPRSLNEFPHMSNQIHNQNISSRYASHRKSLQIPIPFPLLVNKFIPLLSPVIPHCKKAF 
Sbjct:   64 FIDSKNMNPNNFPYDSHNFMLDNCKSSCNNFPVQYGNRFVESVCPNNQYYHNFTNNSYMHSPYIIYDPRTNSFQNVLLVPQMMPMINHSIDMNEENQFSLQRLHNNGRNIQNCPYPDYKDYPVQESFPLNDQYQNLENQVFSETNGFSYNNLGGYISHDFNEGEEDVNEIHQTELESLHSVNRNISGDSEDYTSEDDQLNTVHGDEMLEELQKLSSVFTNVVNVSIMSRNSNISEPVELLNINNSSRFKKTRIKERTTYWNNKQAIVKDNLVKIIPSEILKIPFIDNETLNRFENEIFGDQLAEK--EEKIFEQPDCIHSTLHCPGGFNDLRIHKHSNRTSQRCRSTATPRSLNEFPHMSNQIHNQNISSRYASHRKSLQIPIPFPLLVNKFIPLLSPVIPHCKKAFL 469
[Close]
BLAST of SMED30016357 vs. Planmine SMEST
Match: SMESG000012927.1 (SMESG000012927.1)

HSP 1 Score: 630.172 bits (1624), Expect = 0.000e+0
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 1 
Query: 1435 FIDSKNMNPNNFPYDSHNFMLDNCKSSCNNFPVQYGNRFVESVCPNNQYYHNFTNNSYMHSPYIIYDPRTNSFQNVLLVPQMMPMINHSIDMNEENQFSLQRLHNNGRNIQNCPYPDYKDYPVQESFPLNDQYQNLENQVFSETNGFSYNNLGGYISHDFNEGEEDVNEIHQTELESLHSVNRNISGDSEDYTSEDDQLNTVHGDEMLEELQKLSSVFTNVVNVSIMSRNSNISEPVELLNINNSSRFKKTRIKERTTYWNNKQAIVKDNLVKIIPSEILKIPFIDNETLNRFENEIFGDQLAEK 2349
            FIDSKNMNPNNFPYDSHNFMLDNCKSSCNNFPVQYGNRFVESVCPNNQYYHNFTNNSYMHSPYIIYDPRTNSFQNVLLVPQMMPMINHSIDMNEENQFSLQRLHNNGRNIQNCPYPDYKDYPVQESFPLNDQYQNLENQVFSETNGFSYNNLGGYISHDFNEGEEDVNEIHQTELESLHSVNRNISGDSEDYTSEDDQLNTVHGDEMLEELQKLSSVFTNVVNVSIMSRNSNISEPVELLNINNSSRFKKTRIKERTTYWNNKQAIVKDNLVKIIPSEILKIPFIDNETLNRFENEIFGDQLAEK
Sbjct:   60 FIDSKNMNPNNFPYDSHNFMLDNCKSSCNNFPVQYGNRFVESVCPNNQYYHNFTNNSYMHSPYIIYDPRTNSFQNVLLVPQMMPMINHSIDMNEENQFSLQRLHNNGRNIQNCPYPDYKDYPVQESFPLNDQYQNLENQVFSETNGFSYNNLGGYISHDFNEGEEDVNEIHQTELESLHSVNRNISGDSEDYTSEDDQLNTVHGDEMLEELQKLSSVFTNVVNVSIMSRNSNISEPVELLNINNSSRFKKTRIKERTTYWNNKQAIVKDNLVKIIPSEILKIPFIDNETLNRFENEIFGDQLAEK 364
[Close]
BLAST of SMED30016357 vs. Planmine SMEST
Match: SMESG000012927.1 (SMESG000012927.1)

HSP 1 Score: 630.172 bits (1624), Expect = 0.000e+0
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 1 
Query: 1435 FIDSKNMNPNNFPYDSHNFMLDNCKSSCNNFPVQYGNRFVESVCPNNQYYHNFTNNSYMHSPYIIYDPRTNSFQNVLLVPQMMPMINHSIDMNEENQFSLQRLHNNGRNIQNCPYPDYKDYPVQESFPLNDQYQNLENQVFSETNGFSYNNLGGYISHDFNEGEEDVNEIHQTELESLHSVNRNISGDSEDYTSEDDQLNTVHGDEMLEELQKLSSVFTNVVNVSIMSRNSNISEPVELLNINNSSRFKKTRIKERTTYWNNKQAIVKDNLVKIIPSEILKIPFIDNETLNRFENEIFGDQLAEK 2349
            FIDSKNMNPNNFPYDSHNFMLDNCKSSCNNFPVQYGNRFVESVCPNNQYYHNFTNNSYMHSPYIIYDPRTNSFQNVLLVPQMMPMINHSIDMNEENQFSLQRLHNNGRNIQNCPYPDYKDYPVQESFPLNDQYQNLENQVFSETNGFSYNNLGGYISHDFNEGEEDVNEIHQTELESLHSVNRNISGDSEDYTSEDDQLNTVHGDEMLEELQKLSSVFTNVVNVSIMSRNSNISEPVELLNINNSSRFKKTRIKERTTYWNNKQAIVKDNLVKIIPSEILKIPFIDNETLNRFENEIFGDQLAEK
Sbjct:   59 FIDSKNMNPNNFPYDSHNFMLDNCKSSCNNFPVQYGNRFVESVCPNNQYYHNFTNNSYMHSPYIIYDPRTNSFQNVLLVPQMMPMINHSIDMNEENQFSLQRLHNNGRNIQNCPYPDYKDYPVQESFPLNDQYQNLENQVFSETNGFSYNNLGGYISHDFNEGEEDVNEIHQTELESLHSVNRNISGDSEDYTSEDDQLNTVHGDEMLEELQKLSSVFTNVVNVSIMSRNSNISEPVELLNINNSSRFKKTRIKERTTYWNNKQAIVKDNLVKIIPSEILKIPFIDNETLNRFENEIFGDQLAEK 363
[Close]
BLAST of SMED30016357 vs. Planmine SMEST
Match: SMESG000027427.1 (SMESG000027427.1)

HSP 1 Score: 581.637 bits (1498), Expect = 0.000e+0
Identity = 357/357 (100.00%), Postives = 357/357 (100.00%), Query Frame = 1 
Query:  292 MTSILKSDSNGKNGIILKESTKKFVEATSTSGCDSEMSEITSSIPNSLSLNAIXXXXXXXXXXXXXFSDTKLCEMPEIAKSSSSIEITYELEDIIDGFSIFSFTTDDDLMKFSIKHEKVELLTKSDSNNKIKKFSRTKTKSKIFKNAKESTCNDECIDIYDFQANXXXXXXXXXXXXXXXXXINKSHSNKYSIKSKYKFKXXXXXXXXXXXXXXXXXXXXXXLSIVDGHNDSPQPTSNDDNNVVSTTTPDNLNIEIRKSDSLNNSGKSHSPALKTASLRLFKLEDELTMPSLNRNDKNTNNSVPSASSIGSVNEKISNSQLRLHCADEKFQSKIVPTKFSVDLLTTVTDRQSVVIDE 1362
            MTSILKSDSNGKNGIILKESTKKFVEATSTSGCDSEMSEITSSIPNSLSLNAINNETLTKTNELNKFSDTKLCEMPEIAKSSSSIEITYELEDIIDGFSIFSFTTDDDLMKFSIKHEKVELLTKSDSNNKIKKFSRTKTKSKIFKNAKESTCNDECIDIYDFQANSESSPSSKHSSVVFKKSINKSHSNKYSIKSKYKFKNENNSSLSEENSSNTSVTESHNLSIVDGHNDSPQPTSNDDNNVVSTTTPDNLNIEIRKSDSLNNSGKSHSPALKTASLRLFKLEDELTMPSLNRNDKNTNNSVPSASSIGSVNEKISNSQLRLHCADEKFQSKIVPTKFSVDLLTTVTDRQSVVIDE
Sbjct:    1 MTSILKSDSNGKNGIILKESTKKFVEATSTSGCDSEMSEITSSIPNSLSLNAINNETLTKTNELNKFSDTKLCEMPEIAKSSSSIEITYELEDIIDGFSIFSFTTDDDLMKFSIKHEKVELLTKSDSNNKIKKFSRTKTKSKIFKNAKESTCNDECIDIYDFQANSESSPSSKHSSVVFKKSINKSHSNKYSIKSKYKFKNENNSSLSEENSSNTSVTESHNLSIVDGHNDSPQPTSNDDNNVVSTTTPDNLNIEIRKSDSLNNSGKSHSPALKTASLRLFKLEDELTMPSLNRNDKNTNNSVPSASSIGSVNEKISNSQLRLHCADEKFQSKIVPTKFSVDLLTTVTDRQSVVIDE 357
[Close]
BLAST of SMED30016357 vs. Planmine SMEST
Match: SMESG000012926.1 (SMESG000012926.1)

HSP 1 Score: 245.358 bits (625), Expect = 6.056e-75
Identity = 130/134 (97.01%), Postives = 132/134 (98.51%), Query Frame = 1 
Query: 2341 AEKDSEEKIFEQPDCIHSTLHCPGGFNDLRIHKHSNRTSQRCRSTATPRSLNEFPHMSNQIHNQNISSRYASHRKSLQIPIPFPLLVNKFIPLLSPVIPHCKKAFFEXXXXXXXXXXXXXWSVSCSRLDAGSSN 2742
            A+  S+EKIFEQPDCIHSTLHCPGGFNDLRIHKHSNRTSQRCRSTATPRSLNEFPHMSNQIHNQNISSRYASHRKSLQIPIPFPLLVNKFIPLLSPVIPHCKKAFFEKSSSNNISRRKRRWSVSCSRLDAGSSN
Sbjct:   13 AQLFSKEKIFEQPDCIHSTLHCPGGFNDLRIHKHSNRTSQRCRSTATPRSLNEFPHMSNQIHNQNISSRYASHRKSLQIPIPFPLLVNKFIPLLSPVIPHCKKAFFEKSSSNNISRRKRRWSVSCSRLDAGSSN 146
The following BLAST results are available for this feature:
BLAST of SMED30016357 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016357 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016357 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016357 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016357 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016357 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016357 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016357 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016357 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016357 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016357 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016357 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016357 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016357 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000047715.10.000e+099.26SMESG000047715.1[more]
SMESG000012927.10.000e+0100.00SMESG000012927.1[more]
SMESG000012927.10.000e+0100.00SMESG000012927.1[more]
SMESG000027427.10.000e+0100.00SMESG000027427.1[more]
SMESG000012926.16.056e-7597.01SMESG000012926.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016357 ID=SMED30016357|Name=SMED30016357|organism=Schmidtea mediterranea sexual|type=transcript|length=4095bp
TGTTTATTGTAAAATGTTCTTGTAGAATATTTAAAATTAATTAATCATAA
CGTAAAAGAAAAAAGATATTCAAATAAACCGAAAATCTATATAACGCAAA
TACCCAGCTGAGTAAAGTAGGTCAGTTAAGGCAAATAACTGTCACAATTA
GTAGATTAACTAATATTGGCCTTCCATCTTTTAACTTTATTAATTTATCT
AATGAATTTCAAATTGGATTTTATTTCAGTTCTGTGATTTAACATTGACA
ATAAATATATTTATTTTCTTATCGTTCAACTGATATTTAGAATGACTTCG
ATTTTGAAATCTGACTCGAATGGTAAAAATGGAATTATTTTAAAAGAATC
AACAAAAAAATTCGTCGAAGCAACATCGACATCAGGTTGCGACTCGGAAA
TGTCAGAAATCACTTCATCTATCCCAAATTCTTTGTCATTAAATGCAATA
AACAATGAAACTTTAACAAAAACCAATGAATTGAATAAATTTTCTGACAC
CAAACTCTGTGAAATGCCCGAAATAGCAAAATCATCCTCGTCTATTGAAA
TAACTTATGAATTAGAAGACATTATAGATGGATTTTCAATATTTTCTTTT
ACCACAGATGATGATTTAATGAAATTTTCCATTAAACACGAAAAAGTCGA
ATTGCTTACCAAATCAGACAGTAATAACAAAATCAAGAAATTTTCTCGGA
CAAAAACCAAAAGCAAAATATTCAAGAATGCTAAGGAGTCGACTTGCAAT
GATGAATGCATAGATATTTACGATTTTCAAGCGAATTCCGAATCTTCACC
GTCTAGCAAGCATTCAAGTGTTGTATTTAAAAAGTCTATAAATAAAAGTC
ACTCAAACAAATATTCTATTAAATCGAAATACAAATTCAAAAATGAAAAC
AATTCTTCATTGAGCGAAGAAAACAGTTCAAATACAAGTGTTACTGAATC
TCACAATTTGTCCATCGTTGACGGGCACAATGACAGTCCCCAGCCGACCA
GCAATGATGACAATAACGTCGTTAGCACAACTACACCCGATAATTTGAAT
ATCGAGATTAGAAAATCCGATAGTTTGAATAACTCGGGGAAAAGTCATTC
TCCTGCATTGAAAACTGCTTCATTGAGGCTTTTTAAGTTGGAAGACGAGC
TTACAATGCCTAGTCTTAACCGAAACGACAAAAATACCAATAACAGTGTA
CCCTCTGCCTCGTCTATTGGATCAGTCAATGAGAAGATATCAAACAGTCA
ATTGAGATTGCATTGTGCCGATGAAAAGTTTCAATCGAAAATTGTGCCGA
CAAAATTTTCCGTTGATTTGCTCACAACAGTTACTGATAGGCAATCCGTA
GTGATAGATGAATCCAATTTATGCCTTAACAACAACAGCAACAATATTTG
TAGTAATAATAATAACAATAATAATAATAACAATTTCATTGACTCAAAAA
ATATGAATCCCAACAATTTTCCATATGATTCCCATAATTTTATGCTGGAT
AATTGTAAATCTTCATGCAACAATTTTCCTGTCCAATATGGGAATAGATT
CGTTGAGTCTGTTTGTCCAAATAACCAATATTATCATAATTTCACTAACA
ATTCTTACATGCACTCTCCGTACATCATATATGATCCACGGACGAATTCA
TTTCAAAATGTTTTATTAGTTCCCCAAATGATGCCTATGATAAATCATTC
GATTGACATGAATGAAGAAAACCAATTCTCATTGCAACGTCTCCATAATA
ATGGAAGAAACATACAAAATTGTCCTTATCCCGACTATAAAGATTATCCA
GTGCAAGAAAGTTTTCCTCTAAATGATCAATATCAAAATTTAGAAAATCA
AGTTTTTAGCGAAACCAACGGGTTTTCCTATAATAATTTGGGGGGTTATA
TTTCTCATGATTTTAATGAAGGTGAAGAAGACGTCAATGAAATCCACCAA
ACTGAATTGGAATCATTACATTCAGTTAACAGAAATATCAGCGGAGATTC
GGAGGATTATACATCCGAGGACGATCAGTTAAATACTGTTCACGGAGATG
AAATGCTGGAAGAGCTGCAAAAACTGTCTTCTGTTTTTACTAATGTCGTT
AATGTTTCTATAATGTCTAGAAACAGCAATATATCAGAGCCTGTAGAGTT
ATTGAACATTAACAATTCATCCAGATTCAAAAAGACAAGGATTAAAGAAC
GTACAACTTATTGGAACAACAAACAAGCCATAGTCAAGGATAATCTAGTA
AAAATTATTCCCTCAGAAATTCTAAAAATTCCATTTATTGATAACGAGAC
ATTGAACCGATTTGAAAATGAGATTTTCGGTGATCAACTTGCTGAAAAAG
ATTCAGAAGAAAAAATATTTGAGCAGCCAGATTGCATTCATTCCACATTG
CATTGTCCTGGTGGATTCAATGATTTGAGAATTCACAAACATTCCAATCG
AACTAGTCAAAGGTGTCGATCAACTGCTACTCCTCGTTCCTTAAACGAAT
TTCCGCACATGTCCAATCAGATACATAATCAAAACATATCATCAAGATAC
GCTAGTCATAGAAAATCATTACAGATTCCTATTCCATTTCCGTTGCTCGT
TAACAAATTCATTCCGTTATTGAGTCCAGTAATCCCACATTGTAAAAAAG
CATTTTTTGAGAAGTCAAGCTCAAACAATATCAGTCGTAGAAAACGTCGT
TGGTCAGTAAGCTGTAGTCGTCTTGATGCAGGAAGCTCAAACAGTCCTCG
TGCGAATTTGGTGAATCATCATAGTGAGTCGCCTCGTGATCCATTCAATG
ATTTTCTTGAAGTTTCACAGTCTGAAGAGCTGTCGGAAAAGGATTCCCAC
AATTTTATCAATTCATTCGAATCCCGGATTGATCACCATTTGTTTTGCCT
TAAAAGAACTCTCATAGCTCAGTTTGACCTATCGCTCAACGACATGAGAG
GTGAATTGAAATCGATGAAAAATACTCAGATTGAATTGGAATCTCAGTTG
GTGTTCTACAAGAATAAATTGGATTCCATGGAAAAATCGATTAAAAATTC
TCTGCCAGAGGATGAATGGAAATCATTTGGAATCGATGTTGATTTAGAGA
GTTGAATTGAATAAAATTGTGTTTGTTTTTTTGGTCCCATAGAGACTGAA
GCATTGATCTACGAATCCAACATGAAACTCTGTGAGTTTTGTGCTGGATG
ATTCTGTGGATTTGCAAACCGGTTCAATTAATATTTGATTTCTATCGTCA
TTTCTGATTTATTTATCTGTAAAGAGTTTTCATGTTATTTTTTTAAATGA
CTATTGAAACAGCACTGAATTAAGCATCAGTAACAATTGATCCCATTATT
ATTAATTTTTACATTTATAATTGTTGTTTTGTTGAAATGATCTTTTTCTT
TTGATTTTCTTCAATCCGTTTGTGTTTATTTATTTGTTTTTTTTTGCTGC
GATCACTATTGGGAATAAAAATATTTTTTTAAAATGATTTTCTTAGTCCT
GATTTTAGGTGGATTTTCGGTGTAAGTAATTAAATTTTAAAAAATGTGGC
TAAATTTTTTAAAAACAAAATAGATTGATGAAACCGCAGAAATTGGATAA
TTTTCTTTCAATTAATTGTGATTCGTTTATTTTGTTTTTATTTCATCAGT
TTTGAGAACTTTGTTTAAATGGTTTAGTTTGTTTGTGTGTTTTTTTTCTG
GGATGACAGATTTTTTATACATATATTGGAAACCGCCTCTAAATGGGTGT
CGATGTTCCTTTTTCGTTAGTTGATGGGAAAAACCAAATCAAATATGGAG
AGATTCTAGTCTTGCCGTGTTTTAATTGTGATAAAGTAAATGTTATTGTT
AATTTTTGTTATTTCAATTTTATATGATTTGTTTCAGTCAACAAATCATA
GAGTGATTTTTTTTTAAAAACCTGTATGAATTTTTATAATAATGGGCTTT
ACTTGAGATTTTTGGAGTTTTTTAATCGAGTTCCGGGTCTAGTTTTTTCG
ATTTGCGTGGCGGAAATCAATGTATTTTATTAATAAATAGGAAATGCAAA
TGTTATCAACATAGAATTAGTAAAATTTCAAGATCAACTTTCCTT
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protein sequence of SMED30016357-orf-1

>SMED30016357-orf-1 ID=SMED30016357-orf-1|Name=SMED30016357-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=938bp
MTSILKSDSNGKNGIILKESTKKFVEATSTSGCDSEMSEITSSIPNSLSL
NAINNETLTKTNELNKFSDTKLCEMPEIAKSSSSIEITYELEDIIDGFSI
FSFTTDDDLMKFSIKHEKVELLTKSDSNNKIKKFSRTKTKSKIFKNAKES
TCNDECIDIYDFQANSESSPSSKHSSVVFKKSINKSHSNKYSIKSKYKFK
NENNSSLSEENSSNTSVTESHNLSIVDGHNDSPQPTSNDDNNVVSTTTPD
NLNIEIRKSDSLNNSGKSHSPALKTASLRLFKLEDELTMPSLNRNDKNTN
NSVPSASSIGSVNEKISNSQLRLHCADEKFQSKIVPTKFSVDLLTTVTDR
QSVVIDESNLCLNNNSNNICSNNNNNNNNNNFIDSKNMNPNNFPYDSHNF
MLDNCKSSCNNFPVQYGNRFVESVCPNNQYYHNFTNNSYMHSPYIIYDPR
TNSFQNVLLVPQMMPMINHSIDMNEENQFSLQRLHNNGRNIQNCPYPDYK
DYPVQESFPLNDQYQNLENQVFSETNGFSYNNLGGYISHDFNEGEEDVNE
IHQTELESLHSVNRNISGDSEDYTSEDDQLNTVHGDEMLEELQKLSSVFT
NVVNVSIMSRNSNISEPVELLNINNSSRFKKTRIKERTTYWNNKQAIVKD
NLVKIIPSEILKIPFIDNETLNRFENEIFGDQLAEKDSEEKIFEQPDCIH
STLHCPGGFNDLRIHKHSNRTSQRCRSTATPRSLNEFPHMSNQIHNQNIS
SRYASHRKSLQIPIPFPLLVNKFIPLLSPVIPHCKKAFFEKSSSNNISRR
KRRWSVSCSRLDAGSSNSPRANLVNHHSESPRDPFNDFLEVSQSEELSEK
DSHNFINSFESRIDHHLFCLKRTLIAQFDLSLNDMRGELKSMKNTQIELE
SQLVFYKNKLDSMEKSIKNSLPEDEWKSFGIDVDLES*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR019774Aromatic-AA_hydroxylase_C
Vocabulary: biological process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: molecular function
TermDefinition
GO:0016714oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 247..271
NoneNo IPR availableCOILSCoilCoilcoord: 507..541