SMED30016351

Overview
NameSMED30016351
Smed IDSMED30016351
Length (bp)612
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30016351 (SMED30016351) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30016351 (SMED30016351) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30016351 (SMED30016351) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30016351 (SMED30016351) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30016351

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30016351 SmedASXL_007355SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30016351 SmedASXL_007355SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
gutSMED30016351 dd_Smed_v4_2295_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016351 dd_Smed_v4_2295_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016351 dd_Smed_v4_2295_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30016351 dd_Smed_v4_2295_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30016351 dd_Smed_v4_2295_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016351 Contig8384newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016351 Contig8384uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
parapharyngeal regionSMED30016351 dd_Smed_v6_2295_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
neoblastSMED30016351 SMED_29618_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30016351 SMED_29618_V2_1GPL14150PMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Alignments
SMED30016351 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001959:42594..43416 -2960
Homology
[Close]
BLAST of SMED30016351 vs. Ensembl Human
Match: NOLC1 (nucleolar and coiled-body phosphoprotein 1 [Source:HGNC Symbol;Acc:HGNC:15608])

HSP 1 Score: 63.1586 bits (152), Expect = 3.450e-11
Identity = 39/79 (49.37%), Postives = 54/79 (68.35%), Query Frame = -2 
Query:   27 KRRSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 260
            KR SSPFRR+R+E+   D +   NSF   + ++   G +A Q LK+T+GKSF+ EK K+KR S  GG IS+Q NS+KF+
Sbjct:  628 KRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFD 706
[Close]
BLAST of SMED30016351 vs. Ensembl Human
Match: NOLC1 (nucleolar and coiled-body phosphoprotein 1 [Source:HGNC Symbol;Acc:HGNC:15608])

HSP 1 Score: 63.1586 bits (152), Expect = 3.451e-11
Identity = 39/79 (49.37%), Postives = 54/79 (68.35%), Query Frame = -2 
Query:   27 KRRSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 260
            KR SSPFRR+R+E+   D +   NSF   + ++   G +A Q LK+T+GKSF+ EK K+KR S  GG IS+Q NS+KF+
Sbjct:  629 KRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFD 707
[Close]
BLAST of SMED30016351 vs. Ensembl Human
Match: NOLC1 (nucleolar and coiled-body phosphoprotein 1 [Source:HGNC Symbol;Acc:HGNC:15608])

HSP 1 Score: 63.1586 bits (152), Expect = 3.606e-11
Identity = 39/79 (49.37%), Postives = 54/79 (68.35%), Query Frame = -2 
Query:   27 KRRSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 260
            KR SSPFRR+R+E+   D +   NSF   + ++   G +A Q LK+T+GKSF+ EK K+KR S  GG IS+Q NS+KF+
Sbjct:  619 KRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFD 697
[Close]
BLAST of SMED30016351 vs. Ensembl Human
Match: NOLC1 (nucleolar and coiled-body phosphoprotein 1 [Source:HGNC Symbol;Acc:HGNC:15608])

HSP 1 Score: 63.1586 bits (152), Expect = 3.818e-11
Identity = 39/79 (49.37%), Postives = 54/79 (68.35%), Query Frame = -2 
Query:   27 KRRSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 260
            KR SSPFRR+R+E+   D +   NSF   + ++   G +A Q LK+T+GKSF+ EK K+KR S  GG IS+Q NS+KF+
Sbjct:  620 KRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFD 698
[Close]
BLAST of SMED30016351 vs. Ensembl Fly
Match: Nopp140 (gene:FBgn0037137 transcript:FBtr0332812)

HSP 1 Score: 56.6102 bits (135), Expect = 3.033e-9
Identity = 37/77 (48.05%), Postives = 49/77 (63.64%), Query Frame = -2 
Query:   27 RSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 254
            R SPFRR+R ED   D +    SF   KN++   G +A + LK+T+GKSFK EK K+KR S  GG I +  NS+KF+
Sbjct:  609 RRSPFRRVRTEDVVVDSRVQDMSFEAKKNAAGSWGERANKDLKHTRGKSFKHEKTKKKRGSYRGGQIDVGVNSIKFD 685
[Close]
BLAST of SMED30016351 vs. Ensembl Fly
Match: Nopp140 (gene:FBgn0037137 transcript:FBtr0089579)

HSP 1 Score: 56.225 bits (134), Expect = 3.303e-9
Identity = 37/77 (48.05%), Postives = 49/77 (63.64%), Query Frame = -2 
Query:   27 RSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 254
            R SPFRR+R ED   D +    SF   KN++   G +A + LK+T+GKSFK EK K+KR S  GG I +  NS+KF+
Sbjct:  610 RRSPFRRVRTEDVVVDSRVQDMSFEAKKNAAGSWGERANKDLKHTRGKSFKHEKTKKKRGSYRGGQIDVGVNSIKFD 686
[Close]
BLAST of SMED30016351 vs. Ensembl Zebrafish
Match: nolc1 (nucleolar and coiled-body phosphoprotein 1 [Source:ZFIN;Acc:ZDB-GENE-030131-6349])

HSP 1 Score: 52.373 bits (124), Expect = 9.983e-8
Identity = 35/75 (46.67%), Postives = 47/75 (62.67%), Query Frame = -2 
Query:   27 SPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 248
            +PFRRIR+ED   D +   NSF     ++   G +A   LK+T+GKSF+ EK K+KR S  GG IS   NS+KF+
Sbjct:  925 APFRRIREEDVEVDPRLKDNSFDAKMGANGDWGQKANNVLKFTKGKSFRHEKTKKKRGSYRGGAISTTVNSIKFD 999
[Close]
BLAST of SMED30016351 vs. Ensembl Xenopus
Match: nolc1 (nucleolar and coiled-body phosphoprotein 1 [Source:NCBI gene;Acc:395027])

HSP 1 Score: 50.8322 bits (120), Expect = 5.152e-7
Identity = 32/77 (41.56%), Postives = 48/77 (62.34%), Query Frame = -2 
Query:   27 RSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 254
            +++PFRR+R+E+   +     N+F   K +    G +A   LK+T+GKSF+ EK K+KR S  GG IS   NS+KF+
Sbjct:  839 KNTPFRRVREEEVEINPLMTDNAFEAKKGAQGDWGEKANNVLKFTKGKSFRHEKTKKKRGSYCGGAISTSVNSIKFD 915
[Close]
BLAST of SMED30016351 vs. Ensembl Mouse
Match: Nolc1 (nucleolar and coiled-body phosphoprotein 1 [Source:MGI Symbol;Acc:MGI:1918019])

HSP 1 Score: 62.3882 bits (150), Expect = 4.329e-11
Identity = 39/79 (49.37%), Postives = 54/79 (68.35%), Query Frame = -2 
Query:   27 KRRSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 260
            +R SSPFRR+R+E+   D +   NSF   + ++   G +A Q LK+T+GKSF+ EK K+KR S  GG IS+Q NSVKF+
Sbjct:  620 RRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSVKFD 698
[Close]
BLAST of SMED30016351 vs. Ensembl Mouse
Match: Nolc1 (nucleolar and coiled-body phosphoprotein 1 [Source:MGI Symbol;Acc:MGI:1918019])

HSP 1 Score: 62.3882 bits (150), Expect = 4.413e-11
Identity = 39/79 (49.37%), Postives = 54/79 (68.35%), Query Frame = -2 
Query:   27 KRRSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 260
            +R SSPFRR+R+E+   D +   NSF   + ++   G +A Q LK+T+GKSF+ EK K+KR S  GG IS+Q NSVKF+
Sbjct:  621 RRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSVKFD 699
[Close]
BLAST of SMED30016351 vs. Ensembl Mouse
Match: Nolc1 (nucleolar and coiled-body phosphoprotein 1 [Source:MGI Symbol;Acc:MGI:1918019])

HSP 1 Score: 62.3882 bits (150), Expect = 4.716e-11
Identity = 39/79 (49.37%), Postives = 54/79 (68.35%), Query Frame = -2 
Query:   27 KRRSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 260
            +R SSPFRR+R+E+   D +   NSF   + ++   G +A Q LK+T+GKSF+ EK K+KR S  GG IS+Q NSVKF+
Sbjct:  622 RRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSVKFD 700
[Close]
BLAST of SMED30016351 vs. Ensembl Mouse
Match: Nolc1 (nucleolar and coiled-body phosphoprotein 1 [Source:MGI Symbol;Acc:MGI:1918019])

HSP 1 Score: 62.3882 bits (150), Expect = 4.716e-11
Identity = 39/79 (49.37%), Postives = 54/79 (68.35%), Query Frame = -2 
Query:   27 KRRSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 260
            +R SSPFRR+R+E+   D +   NSF   + ++   G +A Q LK+T+GKSF+ EK K+KR S  GG IS+Q NSVKF+
Sbjct:  622 RRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSVKFD 700
[Close]
BLAST of SMED30016351 vs. Ensembl Mouse
Match: Nolc1 (nucleolar and coiled-body phosphoprotein 1 [Source:MGI Symbol;Acc:MGI:1918019])

HSP 1 Score: 62.3882 bits (150), Expect = 4.761e-11
Identity = 39/79 (49.37%), Postives = 54/79 (68.35%), Query Frame = -2 
Query:   27 KRRSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 260
            +R SSPFRR+R+E+   D +   NSF   + ++   G +A Q LK+T+GKSF+ EK K+KR S  GG IS+Q NSVKF+
Sbjct:  622 RRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSVKFD 700
[Close]
BLAST of SMED30016351 vs. UniProt/SwissProt
Match: sp|P41777|NOLC1_RAT (Nucleolar and coiled-body phosphoprotein 1 OS=Rattus norvegicus OX=10116 GN=Nolc1 PE=1 SV=2)

HSP 1 Score: 63.1586 bits (152), Expect = 1.477e-10
Identity = 40/79 (50.63%), Postives = 54/79 (68.35%), Query Frame = -2 
Query:   27 KRRSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 260
            KR SSPFRR+R+E+   D +   NSF   + ++   G +A Q LK+T+GKSF+ EK K+KR S  GG IS+Q NSVKF+
Sbjct:  624 KRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSVKFD 702
[Close]
BLAST of SMED30016351 vs. UniProt/SwissProt
Match: sp|Q14978|NOLC1_HUMAN (Nucleolar and coiled-body phosphoprotein 1 OS=Homo sapiens OX=9606 GN=NOLC1 PE=1 SV=2)

HSP 1 Score: 63.1586 bits (152), Expect = 1.732e-10
Identity = 39/79 (49.37%), Postives = 54/79 (68.35%), Query Frame = -2 
Query:   27 KRRSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 260
            KR SSPFRR+R+E+   D +   NSF   + ++   G +A Q LK+T+GKSF+ EK K+KR S  GG IS+Q NS+KF+
Sbjct:  619 KRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFD 697
[Close]
BLAST of SMED30016351 vs. UniProt/SwissProt
Match: sp|E9Q5C9|NOLC1_MOUSE (Nucleolar and coiled-body phosphoprotein 1 OS=Mus musculus OX=10090 GN=Nolc1 PE=1 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 3.299e-10
Identity = 39/79 (49.37%), Postives = 54/79 (68.35%), Query Frame = -2 
Query:   27 KRRSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 260
            +R SSPFRR+R+E+   D +   NSF   + ++   G +A Q LK+T+GKSF+ EK K+KR S  GG IS+Q NSVKF+
Sbjct:  622 RRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSVKFD 700
[Close]
BLAST of SMED30016351 vs. TrEMBL
Match: K9KFF5 (Nucleolar phosphoprotein p130-like protein (Fragment) OS=Equus caballus OX=9796 PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 3.554e-10
Identity = 49/114 (42.98%), Postives = 67/114 (58.77%), Query Frame = -2 
Query:   27 KKKFKLDASFVNSTPVEKKIVP------------AKRRSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 329
            KK+ + +A+    TP  KKI P             KR SSPFRRIR+E+   D +   NSF   + ++   G +A Q LK+T+GKSF+ EK K+KR S  GG IS+Q NS+KF+
Sbjct:   13 KKRKQNEAAKEAETPQAKKIKPQTPNTFPKRKKGEKRASSPFRRIREEEIEVDARVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFD 126
[Close]
BLAST of SMED30016351 vs. TrEMBL
Match: U6D0G1 (Nucleolar and coiled-body phosphoprotein 1 (Fragment) OS=Neovison vison OX=452646 GN=NOLC1 PE=2 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 5.348e-10
Identity = 40/79 (50.63%), Postives = 54/79 (68.35%), Query Frame = -2 
Query:   27 KRRSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 260
            KR SSPFRRIR+E+   D +   NSF   + ++   G +A Q LK+T+GKSF+ EK K+KR S  GG IS+Q NS+KF+
Sbjct:    6 KRASSPFRRIREEEIEVDARVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFD 84
[Close]
BLAST of SMED30016351 vs. TrEMBL
Match: L7MRV2 (Nucleolar and coiled-body phosphoprotein 1-like protein (Fragment) OS=Equus caballus OX=9796 GN=NOLC1 PE=2 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 5.348e-10
Identity = 40/79 (50.63%), Postives = 54/79 (68.35%), Query Frame = -2 
Query:   27 KRRSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 260
            KR SSPFRRIR+E+   D +   NSF   + ++   G +A Q LK+T+GKSF+ EK K+KR S  GG IS+Q NS+KF+
Sbjct:    6 KRASSPFRRIREEEIEVDARVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFD 84
[Close]
BLAST of SMED30016351 vs. TrEMBL
Match: L5K0A6 (Nucleolar phosphoprotein p130 OS=Pteropus alecto OX=9402 GN=PAL_GLEAN10014259 PE=4 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 1.442e-9
Identity = 49/114 (42.98%), Postives = 68/114 (59.65%), Query Frame = -2 
Query:   27 KKKFKLDASFVNSTPVEKKIVP------------AKRRSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 329
            KK+ +++A+    TP  KKI P             KR SSPFRRIR+E+   D +   NSF   + ++   G +A Q LK+T+GKSF+ EK K+KR S  GG IS+Q NS+KF+
Sbjct:  591 KKRKQIEAATEEETPQAKKIKPQTPNTFPKRKKAEKRASSPFRRIREEEIEVDARVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFD 704
[Close]
BLAST of SMED30016351 vs. TrEMBL
Match: K9IME6 (Putative nucleolar and coiled-body phosphoprotein 1 OS=Desmodus rotundus OX=9430 PE=2 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 1.869e-9
Identity = 49/114 (42.98%), Postives = 67/114 (58.77%), Query Frame = -2 
Query:   27 KKKFKLDASFVNSTPVEKKIVP------------AKRRSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 329
            KK+ + +A+    TP  KKI P             KR SSPFRR+R+ED   D +   NSF   + ++   G +A Q LK+T+GKSF+ EK K+KR S  GG IS+Q NS+KF+
Sbjct:  600 KKRKQNEAAMQTETPPAKKIKPQTPNTFPKRKKAEKRASSPFRRVREEDVEVDARVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFD 713
[Close]
BLAST of SMED30016351 vs. Ensembl Cavefish
Match: nolc1 (nucleolar and coiled-body phosphoprotein 1 [Source:ZFIN;Acc:ZDB-GENE-030131-6349])

HSP 1 Score: 51.9878 bits (123), Expect = 1.378e-7
Identity = 34/79 (43.04%), Postives = 50/79 (63.29%), Query Frame = -2 
Query:   27 KRRSSPFRRIRDEDFS-DRXXXXXXXXXXXXXXSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 260
            K+ + PFRRI+D++   D +   NSF+    ++   G +A   LK+T+GKSF+ EK K+KR S  GG IS   NS+KF+
Sbjct:  807 KKTNDPFRRIKDDEVEVDPRLADNSFTAKMGATGDWGERANNVLKFTKGKSFRHEKTKKKRGSYRGGAISTSVNSIKFD 885
[Close]
BLAST of SMED30016351 vs. Ensembl Medaka
Match: nolc1 (nucleolar and coiled-body phosphoprotein 1 [Source:NCBI gene;Acc:101162948])

HSP 1 Score: 47.3654 bits (111), Expect = 4.747e-6
Identity = 23/43 (53.49%), Postives = 31/43 (72.09%), Query Frame = -2 
Query:   27 GNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 155
            G +A + L++T+GKSF+ EK K+KR S  GG IS   NS+KFE
Sbjct:  866 GQKANEVLRFTKGKSFRHEKTKKKRGSYRGGAISTSVNSIKFE 908
[Close]
BLAST of SMED30016351 vs. Ensembl Medaka
Match: nolc1 (nucleolar and coiled-body phosphoprotein 1 [Source:NCBI gene;Acc:101162948])

HSP 1 Score: 47.3654 bits (111), Expect = 4.747e-6
Identity = 23/43 (53.49%), Postives = 31/43 (72.09%), Query Frame = -2 
Query:   27 GNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPISLQNNSVKFE 155
            G +A + L++T+GKSF+ EK K+KR S  GG IS   NS+KFE
Sbjct:  866 GQKANEVLRFTKGKSFRHEKTKKKRGSYRGGAISTSVNSIKFE 908
The following BLAST results are available for this feature:
BLAST of SMED30016351 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
NOLC13.450e-1149.37nucleolar and coiled-body phosphoprotein 1 [Source... [more]
NOLC13.451e-1149.37nucleolar and coiled-body phosphoprotein 1 [Source... [more]
NOLC13.606e-1149.37nucleolar and coiled-body phosphoprotein 1 [Source... [more]
NOLC13.818e-1149.37nucleolar and coiled-body phosphoprotein 1 [Source... [more]
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BLAST of SMED30016351 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016351 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Nopp1403.033e-948.05gene:FBgn0037137 transcript:FBtr0332812[more]
Nopp1403.303e-948.05gene:FBgn0037137 transcript:FBtr0089579[more]
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BLAST of SMED30016351 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nolc19.983e-846.67nucleolar and coiled-body phosphoprotein 1 [Source... [more]
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BLAST of SMED30016351 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nolc15.152e-741.56nucleolar and coiled-body phosphoprotein 1 [Source... [more]
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BLAST of SMED30016351 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Nolc14.329e-1149.37nucleolar and coiled-body phosphoprotein 1 [Source... [more]
Nolc14.413e-1149.37nucleolar and coiled-body phosphoprotein 1 [Source... [more]
Nolc14.716e-1149.37nucleolar and coiled-body phosphoprotein 1 [Source... [more]
Nolc14.716e-1149.37nucleolar and coiled-body phosphoprotein 1 [Source... [more]
Nolc14.761e-1149.37nucleolar and coiled-body phosphoprotein 1 [Source... [more]
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BLAST of SMED30016351 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|P41777|NOLC1_RAT1.477e-1050.63Nucleolar and coiled-body phosphoprotein 1 OS=Ratt... [more]
sp|Q14978|NOLC1_HUMAN1.732e-1049.37Nucleolar and coiled-body phosphoprotein 1 OS=Homo... [more]
sp|E9Q5C9|NOLC1_MOUSE3.299e-1049.37Nucleolar and coiled-body phosphoprotein 1 OS=Mus ... [more]
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BLAST of SMED30016351 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
K9KFF53.554e-1042.98Nucleolar phosphoprotein p130-like protein (Fragme... [more]
U6D0G15.348e-1050.63Nucleolar and coiled-body phosphoprotein 1 (Fragme... [more]
L7MRV25.348e-1050.63Nucleolar and coiled-body phosphoprotein 1-like pr... [more]
L5K0A61.442e-942.98Nucleolar phosphoprotein p130 OS=Pteropus alecto O... [more]
K9IME61.869e-942.98Putative nucleolar and coiled-body phosphoprotein ... [more]
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BLAST of SMED30016351 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nolc11.378e-743.04nucleolar and coiled-body phosphoprotein 1 [Source... [more]
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BLAST of SMED30016351 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016351 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016351 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016351 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
nolc14.747e-653.49nucleolar and coiled-body phosphoprotein 1 [Source... [more]
nolc14.747e-653.49nucleolar and coiled-body phosphoprotein 1 [Source... [more]
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BLAST of SMED30016351 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 0
Match NameE-valueIdentityDescription
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016351 ID=SMED30016351|Name=SMED30016351|organism=Schmidtea mediterranea sexual|type=transcript|length=612bp
TTTCTAAATACCAACAATATATTTTATTCAAATTTCACAGAATTATTCTG
TAGACTAATGGGACCACCAATGCAGCTTGAACGTTTTCTTTTATTCTTTT
CTTGTTTAAATGATTTACCTTGAGTGTATTTCAAACTTTGGTACGCTTGA
TTTCCACAAAGAGAGCTTGAGTTTTTTATATTAGAAAATGAATTACTGTC
TTTCTTTCTATCACTGAAATCTTCATCTCTGATACGTCGAAATGGTGAAC
TTCGTCGCTTAGCGGGAACAATTTTCTTTTCCACAGGCGTTGAATTTACA
AAAGAGGCATCCAATTTAAATTTTTTTTTGAACATTCGAATCACTTTCCT
CATGCTTACGTTTCAATTCTTTTTTCCTATTCGAATCAGAGTTTTCATTC
AATTTAATTTTCTTTGAAGAAACGATTTCAGAGTTAGAAATACTGCCAAC
TCTTTTCCTTTGCTTAGATTTATCAAACTTTTTATTCGTTTCCGCAATGA
TAATGGTTTTAATGGGTGCCTTTTCTTTTTCCTCTTCACTGGATGAATCT
TCATCAGATGATTCTTCCTCTTTTTCTACTGCGACAATTGACTTGACTGG
AGCTTTTTCTTC
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protein sequence of SMED30016351-orf-1

>SMED30016351-orf-1 ID=SMED30016351-orf-1|Name=SMED30016351-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=110bp
MRKVIRMFKKKFKLDASFVNSTPVEKKIVPAKRRSSPFRRIRDEDFSDRK
KDSNSFSNIKNSSSLCGNQAYQSLKYTQGKSFKQEKNKRKRSSCIGGPIS
LQNNSVKFE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000101muscle cell
PLANA:0000420parapharyngeal region
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
PLANA:0003001lateral region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR007718SRP40_C
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..37
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 73..99
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..19
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 69..99