GCR158
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30016269 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Homology
BLAST of GCR158 vs. TrEMBL
Match: A0A193KUF9 (GCR158 OS=Schmidtea mediterranea OX=79327 GN=gcr158 PE=2 SV=1) HSP 1 Score: 108.997 bits (271), Expect = 2.817e-23 Identity = 108/296 (36.49%), Postives = 169/296 (57.09%), Query Frame = 3 Query: 162 VLFNSLLLYVVTNRVKFCHDKRTFHKLFQXXXXXXXXXXXXXXXXXXXKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMS--KIWKLEIVMWILEVLLVIIFLSYGVRFILLTINISCSMKKRIDTHGKDIIGISGGVFMMFNLISYMVLFSFLCRRHLYNKQKSINSERLYVSMQMNKSKPVALTSDNETEITKKDSKINLKQISVNRKIGSIKFVKVLLGVNVLMITSFSPAILYNADFIKFPTYSVY-YLFFLHSLGDPILFFLTQKELKNGLISIFK 1040 V NS+LLY+V + F D+++ H LFQ+LI+ DL+IS+LILP+I++K + H LF A RD IS Q+ LV IAID L++ P++ + W L + I L +I + V+ ILL N+ ++K+ I+ K + + G +F++ +I Y LF + RR + KS+ + Q KS+ T +N +E +KI +KQ + ++I +KFVKVL VN+LMI SF P LYN+ +KF Y+ + YLF++HSLGD I+F + +L+ L + + Sbjct: 30 VFLNSILLYLVMKKNYFKFDQQSIHYLFQMLIVCDLLISILILPDILVKKTVTNGKYHPAVILFRAMRDALISIQIFLLVAIAIDGLVSILNPINSRQFWNLSNIKRIFITYLSVIVVLLLVKSILLLSNLDSNVKQNINYAIKFGLSLIGMIFIVMIIIIYSYLFCLIYRRQMDRCDKSMKRK------QCLKSQSTE-TEENFSEDESGRNKITVKQWNFGKRIIHMKFVKVLFWVNLLMIVSFLPGCLYNSGLLKFKAYNWFIYLFYVHSLGDAIIFLFIRLDLRKDLTKLLR 318
BLAST of GCR158 vs. TrEMBL
Match: A0A193KUR6 (GCR156 OS=Schmidtea mediterranea OX=79327 GN=gcr156 PE=2 SV=1) HSP 1 Score: 93.2041 bits (230), Expect = 1.230e-17 Identity = 67/230 (29.13%), Postives = 114/230 (49.57%), Query Frame = 3 Query: 351 FLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYGVRFILLTINISCSMKKRIDTHGKDIIGISGGVFMMFNLISYMVLFSFLCRRHLYNKQKSINSERLYVSMQMNKSKPVALTSDNETEITKKDSKINLKQISVNRKIG-SIKFVKVLLGVNVLMITSFSPAILYNADFIKFPTYSVYYLFFLHSLGDPILFFLTQKELKNGLISIF 1037 FL+ R+ IS ITL+ +A +R A P +++ + ++ V I + + VRF L+ C + + ID K IGI+ + +M + Y VLF LC R+ + K N SK ++ ++ K K R G S+KF +VL + +LM+ S++P L N + IK+P ++YYLF+L LGDP++ L++++++ +I F Sbjct: 98 FLSLRNCLISCHAITLLFMATNRYRAIKHPQMVNDPRSVLIKLYRIIYVTISIPFLVRFALVFCLRDCLLLQEIDNALKMSIGIATALVVMVIICIYSVLFGLLCCRNFPSTSKPTG----------NFSKIHPYPNERKSTTVGKVFK---------RHFGKSVKFAQVLFSITILMMISYAPVFLTNVNLIKYPGQAIYYLFYLPVLGDPLILILSREDIRQEMIRPF 308
BLAST of GCR158 vs. Planmine SMEST
Match: SMESG000062349.1 (SMESG000062349.1) HSP 1 Score: 325.094 bits (832), Expect = 6.752e-110 Identity = 196/323 (60.68%), Postives = 243/323 (75.23%), Query Frame = 3 Query: 84 CRKEDTMKGVVDIFFYPIYSLMFILSVLFNSLLLYVVTNRVKFCHDKRTFHKLFQXXXXXXXXXXXXXXXXXXXKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYGVRFILLTINISCSMKKRIDTHGKDIIGISGGVFMMFNLISYMVLFSFLCRRHLYNKQKSINSERLYVSMQMNK--SKPVALTSDNETEITK--KDSKINLKQISVNRKIGSIKFVKVLLGVNVLMITSFSPAILYNADFIKFPTYSVYYLFFLHSLGDPILFFLTQKELKNGLISIFK 1040 CR DT K ++I FYPIYS MFILSVL NS+LLYVVT + H+KR FHKLFQ LI LDLIIS+LILPNI+IK FT+DTDT+ + D+FLA RD +IS+QMITLVLIAIDRL AF P+S W L VMWIL+VL++ + LSY R ILL+I ISC KK+ID +GK IIGISGGVFM+ NL+ Y+ LFS LC+R L ++ S NS++ Y +MQ P +NET ITK + S +N +++ R+IGSIKF KVLLG+N+LMI S+ P +LYN FI FP+YSVYYLFFLH+LGDPI+F LT++ELKNGL+SI K Sbjct: 7 CRNHDTNKKTIEIAFYPIYSGMFILSVLLNSILLYVVTKIESYAHEKRVFHKLFQFLIFLDLIISILILPNILIKRFTEDTDTNIFLDVFLAIRDLSISAQMITLVLIAIDRLFAFVNPLSNYWVLNTVMWILKVLMLFLSLSYITRLILLSIGISCLNKKKIDKYGKIIIGISGGVFMLINLVIYVSLFSLLCQRQLVRQRNSFNSDKFYRAMQSKSYDENP----EENETSITKSRRISSLNSRKLRFRRRIGSIKFAKVLLGINILMIVSYLPGVLYNNKFIPFPSYSVYYLFFLHALGDPIIFLLTRRELKNGLLSILK 325
BLAST of GCR158 vs. Planmine SMEST
Match: SMESG000062328.1 (SMESG000062328.1) HSP 1 Score: 307.375 bits (786), Expect = 7.481e-103 Identity = 179/323 (55.42%), Postives = 235/323 (72.76%), Query Frame = 3 Query: 84 CRKEDTMKGVVDIFFYPIYSLMFILSVLFNSLLLYVVTNRVKFCHDKRTFHKLFQXXXXXXXXXXXXXXXXXXXKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYGVRFILLTINISCSMKKRIDTHGKDIIGISGGVFMMFNLISYMVLFSFLCRRHLYNKQKSI---NSERLYVSMQMNKSKPVALTSDNETE--ITKKD-SKINLKQISVNRKIGSIKFVKVLLGVNVLMITSFSPAILYNADFIKFPTYSVYYLFFLHSLGDPILFFLTQKELKNGLISI 1034 CRK D K +D FYPIYS+MF+LSV NS+LLY+V + KF H+K++FHKLFQ+LI LDLIIS+LILPNI+IK ++KD DT D +LA RDFAISSQMI LVLIAIDRLLAF +P+S W++ V+ + ++ ++ L Y +RF LLT+NI+C +KK ID+ K IGI GG+ M+ NLI Y +LF+ LCRR ++N + S+ N + L V +KS P N + + + D S++ K I NRKIGSIKFVKVL GVN+LMI S+ PAILYNA+ I+FP++SVYYLFFLH+LGDPILFF+T++EL N L+ I Sbjct: 15 CRKHDIEKNGIDGAFYPIYSIMFVLSVFLNSILLYIVIKKDKFIHEKKSFHKLFQVLIFLDLIISILILPNILIKMYSKDHDTFIVLDGYLAIRDFAISSQMIILVLIAIDRLLAFLLPLSNFWRIGRVLKVFKIFFILTLLFYSIRFTLLTVNINCDIKKSIDSDVKLFIGIFGGILMVINLIIYCILFALLCRRQIFNHKDSLLSANFKSLNVKELESKSIPKIENKKNSSNAMLLQSDFSQVKFKVIPANRKIGSIKFVKVLFGVNLLMIISYLPAILYNAECIQFPSFSVYYLFFLHALGDPILFFMTRRELLNSLLKI 337
BLAST of GCR158 vs. Planmine SMEST
Match: SMESG000062345.1 (SMESG000062345.1) HSP 1 Score: 289.271 bits (739), Expect = 3.849e-98 Identity = 169/187 (90.37%), Postives = 169/187 (90.37%), Query Frame = 3 Query: 51 MNISLGHENGICRKEDTMKGVVDIFFYPIYSLMFILSVLFNSLLLYVVTNRVKFCHDKRTFHKLFQXXXXXXXXXXXXXXXXXXXKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYGVRFILLTINISCSMKKRIDTHGKDIIGISGG 611 MNISLGHENGICRKEDTMK VLFNSLLLYVVTNRVKFCHDKRTFHKLFQILIILDLIISLLILPNIIIKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYGVRFILLTINISCSMKKRIDTHGKDIIGISGG Sbjct: 1 MNISLGHENGICRKEDTMK------------------VLFNSLLLYVVTNRVKFCHDKRTFHKLFQILIILDLIISLLILPNIIIKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYGVRFILLTINISCSMKKRIDTHGKDIIGISGG 169
BLAST of GCR158 vs. Planmine SMEST
Match: SMESG000062345.1 (SMESG000062345.1) HSP 1 Score: 248.44 bits (633), Expect = 2.604e-82 Identity = 153/187 (81.82%), Postives = 153/187 (81.82%), Query Frame = 3 Query: 51 MNISLGHENGICRKEDTMKGVVDIFFYPIYSLMFILSVLFNSLLLYVVTNRVKFCHDKRTFHKLFQXXXXXXXXXXXXXXXXXXXKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYGVRFILLTINISCSMKKRIDTHGKDIIGISGG 611 MNISLGHENGICRKEDTMK VLFNSLLLYVVTNRVKFCHDKRTFHKLFQILIILDLIISLLILPNIIIKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYG RIDTHGKDIIGISGG Sbjct: 1 MNISLGHENGICRKEDTMK------------------VLFNSLLLYVVTNRVKFCHDKRTFHKLFQILIILDLIISLLILPNIIIKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYG----------------RIDTHGKDIIGISGG 153
BLAST of GCR158 vs. Planmine SMEST
Match: SMESG000077660.1 (SMESG000077660.1) HSP 1 Score: 213.386 bits (542), Expect = 2.398e-66 Identity = 144/320 (45.00%), Postives = 199/320 (62.19%), Query Frame = 3 Query: 75 NGICRKEDTMKGVVDIFFYPIYSLMFILSVLFNSLLLYVVTNRVKFCHDKRTFHKLFQXXXXXXXXXXXXXXXXXXXKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYGVRFILLTINISCSMKKRIDTHGKDIIGISGGVFMMFNLISYMVLFSFLCRRHLYNKQKSINSERLYVSMQMNKSKPVALTSDNETEITKKDSKIN--LKQISVNRKIGSIKFVKVLLGVNVLMITSFSPAILYNADFIKFPTYSVYYLFFLHSLGDPILFFLTQKELKNGLI 1028 N CR D K +++ F+P+Y+ MF ++V+ NS+LL++V + + +KRTF KLF +LI LDLIIS+LILPNI IK +T DTDT+ D +LA RDF+ISSQM V IAIDRLL P + ++ +L+ + I L Y +RFI LTI+I C+ K +D + K IGI GGV M NLI Y+VLF LC R +K S++L + ++ + E + ++K + + N+K SI+F K L +N+LMI S+ PAILYN F+ F +YSVYYLFFLH+LGDPILFF T+ ELK L+ Sbjct: 4 NNTCRSHDVKKEDIEVAFFPLYASMFAVAVILNSVLLHLVVKKNRLFLEKRTFDKLFPVLIFLDLIISILILPNIFIKKYTNDTDTYIAVDGYLAVRDFSISSQMTIFVAIAIDRLLTLTHPQITKRRTNTIIKVLQTAVFFIGLFYLLRFIFLTIDIKCNSKVLLDKYMKLFIGIVGGVLMTTNLIIYLVLFGLLCHRQRKESRKFQKSKKLLRTQNSEENVNENQENLIEIPMRPTNNKRSSLKNKFKSNKKSASIRFAKPLFAINLLMIISYLPAILYNVRFVGFHSYSVYYLFFLHALGDPILFFSTRPELKLALL 323 The following BLAST results are available for this feature:
BLAST of GCR158 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of GCR158 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of GCR158 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of GCR158 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of GCR158 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of GCR158 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of GCR158 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of GCR158 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 2
BLAST of GCR158 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of GCR158 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of GCR158 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of GCR158 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of GCR158 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of GCR158 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30016269 ID=SMED30016269|Name=GCR158|organism=Schmidtea mediterranea sexual|type=transcript|length=1119bpback to top protein sequence of SMED30016269-orf-1 >SMED30016269-orf-1 ID=SMED30016269-orf-1|Name=SMED30016269-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=333bp MNISLGHENGICRKEDTMKGVVDIFFYPIYSLMFILSVLFNSLLLYVVTNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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