GCR158

Overview
NameGCR158
Smed IDSMED30016269
Length (bp)1119
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of GCR158 (SMED30016269) t-SNE clustered cells

Violin plots show distribution of expression levels for GCR158 (SMED30016269) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of GCR158 (SMED30016269) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for GCR158 (SMED30016269) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30016269

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30016269h1SMcG0016967 dd_Smed_v4_11380_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016269h1SMcG0016967 dd_Smed_v4_11380_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30016269h1SMcG0016967 dd_Smed_v4_11380_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30016269h1SMcG0016967 dd_Smed_v4_11380_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30016269h1SMcG0016967 dd_Smed_v4_11380_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30016269h1SMcG0016967 dd_Smed_v4_11380_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
enteric muscle cellSMED30016269h1SMcG0016967 dd_Smed_v4_11380_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of GCR158 vs. TrEMBL
Match: A0A193KUF9 (GCR158 OS=Schmidtea mediterranea OX=79327 GN=gcr158 PE=2 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 2.817e-23
Identity = 108/296 (36.49%), Postives = 169/296 (57.09%), Query Frame = 3
Query:  162 VLFNSLLLYVVTNRVKFCHDKRTFHKLFQXXXXXXXXXXXXXXXXXXXKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMS--KIWKLEIVMWILEVLLVIIFLSYGVRFILLTINISCSMKKRIDTHGKDIIGISGGVFMMFNLISYMVLFSFLCRRHLYNKQKSINSERLYVSMQMNKSKPVALTSDNETEITKKDSKINLKQISVNRKIGSIKFVKVLLGVNVLMITSFSPAILYNADFIKFPTYSVY-YLFFLHSLGDPILFFLTQKELKNGLISIFK 1040
            V  NS+LLY+V  +  F  D+++ H LFQ+LI+ DL+IS+LILP+I++K    +   H    LF A RD  IS Q+  LV IAID L++   P++  + W L  +  I    L +I +   V+ ILL  N+  ++K+ I+   K  + + G +F++  +I Y  LF  + RR +    KS+  +      Q  KS+    T +N +E     +KI +KQ +  ++I  +KFVKVL  VN+LMI SF P  LYN+  +KF  Y+ + YLF++HSLGD I+F   + +L+  L  + +
Sbjct:   30 VFLNSILLYLVMKKNYFKFDQQSIHYLFQMLIVCDLLISILILPDILVKKTVTNGKYHPAVILFRAMRDALISIQIFLLVAIAIDGLVSILNPINSRQFWNLSNIKRIFITYLSVIVVLLLVKSILLLSNLDSNVKQNINYAIKFGLSLIGMIFIVMIIIIYSYLFCLIYRRQMDRCDKSMKRK------QCLKSQSTE-TEENFSEDESGRNKITVKQWNFGKRIIHMKFVKVLFWVNLLMIVSFLPGCLYNSGLLKFKAYNWFIYLFYVHSLGDAIIFLFIRLDLRKDLTKLLR 318          
BLAST of GCR158 vs. TrEMBL
Match: A0A193KUR6 (GCR156 OS=Schmidtea mediterranea OX=79327 GN=gcr156 PE=2 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.230e-17
Identity = 67/230 (29.13%), Postives = 114/230 (49.57%), Query Frame = 3
Query:  351 FLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYGVRFILLTINISCSMKKRIDTHGKDIIGISGGVFMMFNLISYMVLFSFLCRRHLYNKQKSINSERLYVSMQMNKSKPVALTSDNETEITKKDSKINLKQISVNRKIG-SIKFVKVLLGVNVLMITSFSPAILYNADFIKFPTYSVYYLFFLHSLGDPILFFLTQKELKNGLISIF 1037
            FL+ R+  IS   ITL+ +A +R  A   P        +++ +  ++ V I + + VRF L+     C + + ID   K  IGI+  + +M  +  Y VLF  LC R+  +  K             N SK     ++ ++    K  K         R  G S+KF +VL  + +LM+ S++P  L N + IK+P  ++YYLF+L  LGDP++  L++++++  +I  F
Sbjct:   98 FLSLRNCLISCHAITLLFMATNRYRAIKHPQMVNDPRSVLIKLYRIIYVTISIPFLVRFALVFCLRDCLLLQEIDNALKMSIGIATALVVMVIICIYSVLFGLLCCRNFPSTSKPTG----------NFSKIHPYPNERKSTTVGKVFK---------RHFGKSVKFAQVLFSITILMMISYAPVFLTNVNLIKYPGQAIYYLFYLPVLGDPLILILSREDIRQEMIRPF 308          
BLAST of GCR158 vs. Planmine SMEST
Match: SMESG000062349.1 (SMESG000062349.1)

HSP 1 Score: 325.094 bits (832), Expect = 6.752e-110
Identity = 196/323 (60.68%), Postives = 243/323 (75.23%), Query Frame = 3
Query:   84 CRKEDTMKGVVDIFFYPIYSLMFILSVLFNSLLLYVVTNRVKFCHDKRTFHKLFQXXXXXXXXXXXXXXXXXXXKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYGVRFILLTINISCSMKKRIDTHGKDIIGISGGVFMMFNLISYMVLFSFLCRRHLYNKQKSINSERLYVSMQMNK--SKPVALTSDNETEITK--KDSKINLKQISVNRKIGSIKFVKVLLGVNVLMITSFSPAILYNADFIKFPTYSVYYLFFLHSLGDPILFFLTQKELKNGLISIFK 1040
            CR  DT K  ++I FYPIYS MFILSVL NS+LLYVVT    + H+KR FHKLFQ LI LDLIIS+LILPNI+IK FT+DTDT+ + D+FLA RD +IS+QMITLVLIAIDRL AF  P+S  W L  VMWIL+VL++ + LSY  R ILL+I ISC  KK+ID +GK IIGISGGVFM+ NL+ Y+ LFS LC+R L  ++ S NS++ Y +MQ       P     +NET ITK  + S +N +++   R+IGSIKF KVLLG+N+LMI S+ P +LYN  FI FP+YSVYYLFFLH+LGDPI+F LT++ELKNGL+SI K
Sbjct:    7 CRNHDTNKKTIEIAFYPIYSGMFILSVLLNSILLYVVTKIESYAHEKRVFHKLFQFLIFLDLIISILILPNILIKRFTEDTDTNIFLDVFLAIRDLSISAQMITLVLIAIDRLFAFVNPLSNYWVLNTVMWILKVLMLFLSLSYITRLILLSIGISCLNKKKIDKYGKIIIGISGGVFMLINLVIYVSLFSLLCQRQLVRQRNSFNSDKFYRAMQSKSYDENP----EENETSITKSRRISSLNSRKLRFRRRIGSIKFAKVLLGINILMIVSYLPGVLYNNKFIPFPSYSVYYLFFLHALGDPIIFLLTRRELKNGLLSILK 325          
BLAST of GCR158 vs. Planmine SMEST
Match: SMESG000062328.1 (SMESG000062328.1)

HSP 1 Score: 307.375 bits (786), Expect = 7.481e-103
Identity = 179/323 (55.42%), Postives = 235/323 (72.76%), Query Frame = 3
Query:   84 CRKEDTMKGVVDIFFYPIYSLMFILSVLFNSLLLYVVTNRVKFCHDKRTFHKLFQXXXXXXXXXXXXXXXXXXXKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYGVRFILLTINISCSMKKRIDTHGKDIIGISGGVFMMFNLISYMVLFSFLCRRHLYNKQKSI---NSERLYVSMQMNKSKPVALTSDNETE--ITKKD-SKINLKQISVNRKIGSIKFVKVLLGVNVLMITSFSPAILYNADFIKFPTYSVYYLFFLHSLGDPILFFLTQKELKNGLISI 1034
            CRK D  K  +D  FYPIYS+MF+LSV  NS+LLY+V  + KF H+K++FHKLFQ+LI LDLIIS+LILPNI+IK ++KD DT    D +LA RDFAISSQMI LVLIAIDRLLAF +P+S  W++  V+ + ++  ++  L Y +RF LLT+NI+C +KK ID+  K  IGI GG+ M+ NLI Y +LF+ LCRR ++N + S+   N + L V    +KS P      N +   + + D S++  K I  NRKIGSIKFVKVL GVN+LMI S+ PAILYNA+ I+FP++SVYYLFFLH+LGDPILFF+T++EL N L+ I
Sbjct:   15 CRKHDIEKNGIDGAFYPIYSIMFVLSVFLNSILLYIVIKKDKFIHEKKSFHKLFQVLIFLDLIISILILPNILIKMYSKDHDTFIVLDGYLAIRDFAISSQMIILVLIAIDRLLAFLLPLSNFWRIGRVLKVFKIFFILTLLFYSIRFTLLTVNINCDIKKSIDSDVKLFIGIFGGILMVINLIIYCILFALLCRRQIFNHKDSLLSANFKSLNVKELESKSIPKIENKKNSSNAMLLQSDFSQVKFKVIPANRKIGSIKFVKVLFGVNLLMIISYLPAILYNAECIQFPSFSVYYLFFLHALGDPILFFMTRRELLNSLLKI 337          
BLAST of GCR158 vs. Planmine SMEST
Match: SMESG000062345.1 (SMESG000062345.1)

HSP 1 Score: 289.271 bits (739), Expect = 3.849e-98
Identity = 169/187 (90.37%), Postives = 169/187 (90.37%), Query Frame = 3
Query:   51 MNISLGHENGICRKEDTMKGVVDIFFYPIYSLMFILSVLFNSLLLYVVTNRVKFCHDKRTFHKLFQXXXXXXXXXXXXXXXXXXXKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYGVRFILLTINISCSMKKRIDTHGKDIIGISGG 611
            MNISLGHENGICRKEDTMK                  VLFNSLLLYVVTNRVKFCHDKRTFHKLFQILIILDLIISLLILPNIIIKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYGVRFILLTINISCSMKKRIDTHGKDIIGISGG
Sbjct:    1 MNISLGHENGICRKEDTMK------------------VLFNSLLLYVVTNRVKFCHDKRTFHKLFQILIILDLIISLLILPNIIIKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYGVRFILLTINISCSMKKRIDTHGKDIIGISGG 169          
BLAST of GCR158 vs. Planmine SMEST
Match: SMESG000062345.1 (SMESG000062345.1)

HSP 1 Score: 248.44 bits (633), Expect = 2.604e-82
Identity = 153/187 (81.82%), Postives = 153/187 (81.82%), Query Frame = 3
Query:   51 MNISLGHENGICRKEDTMKGVVDIFFYPIYSLMFILSVLFNSLLLYVVTNRVKFCHDKRTFHKLFQXXXXXXXXXXXXXXXXXXXKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYGVRFILLTINISCSMKKRIDTHGKDIIGISGG 611
            MNISLGHENGICRKEDTMK                  VLFNSLLLYVVTNRVKFCHDKRTFHKLFQILIILDLIISLLILPNIIIKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYG                RIDTHGKDIIGISGG
Sbjct:    1 MNISLGHENGICRKEDTMK------------------VLFNSLLLYVVTNRVKFCHDKRTFHKLFQILIILDLIISLLILPNIIIKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYG----------------RIDTHGKDIIGISGG 153          
BLAST of GCR158 vs. Planmine SMEST
Match: SMESG000077660.1 (SMESG000077660.1)

HSP 1 Score: 213.386 bits (542), Expect = 2.398e-66
Identity = 144/320 (45.00%), Postives = 199/320 (62.19%), Query Frame = 3
Query:   75 NGICRKEDTMKGVVDIFFYPIYSLMFILSVLFNSLLLYVVTNRVKFCHDKRTFHKLFQXXXXXXXXXXXXXXXXXXXKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYGVRFILLTINISCSMKKRIDTHGKDIIGISGGVFMMFNLISYMVLFSFLCRRHLYNKQKSINSERLYVSMQMNKSKPVALTSDNETEITKKDSKIN--LKQISVNRKIGSIKFVKVLLGVNVLMITSFSPAILYNADFIKFPTYSVYYLFFLHSLGDPILFFLTQKELKNGLI 1028
            N  CR  D  K  +++ F+P+Y+ MF ++V+ NS+LL++V  + +   +KRTF KLF +LI LDLIIS+LILPNI IK +T DTDT+   D +LA RDF+ISSQM   V IAIDRLL    P     +   ++ +L+  +  I L Y +RFI LTI+I C+ K  +D + K  IGI GGV M  NLI Y+VLF  LC R     +K   S++L  +    ++      +  E  +   ++K +    +   N+K  SI+F K L  +N+LMI S+ PAILYN  F+ F +YSVYYLFFLH+LGDPILFF T+ ELK  L+
Sbjct:    4 NNTCRSHDVKKEDIEVAFFPLYASMFAVAVILNSVLLHLVVKKNRLFLEKRTFDKLFPVLIFLDLIISILILPNIFIKKYTNDTDTYIAVDGYLAVRDFSISSQMTIFVAIAIDRLLTLTHPQITKRRTNTIIKVLQTAVFFIGLFYLLRFIFLTIDIKCNSKVLLDKYMKLFIGIVGGVLMTTNLIIYLVLFGLLCHRQRKESRKFQKSKKLLRTQNSEENVNENQENLIEIPMRPTNNKRSSLKNKFKSNKKSASIRFAKPLFAINLLMIISYLPAILYNVRFVGFHSYSVYYLFFLHALGDPILFFSTRPELKLALL 323          
The following BLAST results are available for this feature:
BLAST of GCR158 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 2
Match NameE-valueIdentityDescription
A0A193KUF92.817e-2336.49GCR158 OS=Schmidtea mediterranea OX=79327 GN=gcr15... [more]
A0A193KUR61.230e-1729.13GCR156 OS=Schmidtea mediterranea OX=79327 GN=gcr15... [more]
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BLAST of GCR158 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000062349.16.752e-11060.68SMESG000062349.1[more]
SMESG000062328.17.481e-10355.42SMESG000062328.1[more]
SMESG000062345.13.849e-9890.37SMESG000062345.1[more]
SMESG000062345.12.604e-8281.82SMESG000062345.1[more]
SMESG000077660.12.398e-6645.00SMESG000077660.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016269 ID=SMED30016269|Name=GCR158|organism=Schmidtea mediterranea sexual|type=transcript|length=1119bp
CACGCAATCTATCGGAATGAATTACATTTGGCATCTCATTAATAATTGAA
ATGAATATTAGTCTGGGACACGAAAATGGAATTTGCCGAAAGGAAGATAC
CATGAAAGGTGTAGTCGATATCTTCTTTTACCCAATTTATTCACTAATGT
TTATTTTGTCAGTGTTATTCAACTCACTTCTTCTTTATGTGGTAACAAAC
CGAGTAAAGTTTTGTCATGACAAAAGAACATTTCATAAATTATTTCAAAT
ACTCATAATTCTTGACTTAATTATATCTTTACTAATTTTACCCAATATCA
TAATTAAAGGATTTACGAAAGACACGGATACTCACTGGTACACTGATTTA
TTTCTCGCATCCAGAGATTTCGCGATCTCCAGTCAAATGATCACTTTAGT
CTTGATTGCCATAGATAGATTGTTAGCCTTTTTCGTGCCAATGAGCAAAA
TTTGGAAATTAGAAATTGTCATGTGGATTTTAGAAGTATTATTGGTAATT
ATATTTCTAAGTTATGGAGTAAGATTTATTTTATTAACCATTAATATTTC
TTGTTCAATGAAAAAGAGGATAGACACTCACGGAAAAGATATTATTGGAA
TATCAGGAGGTGTATTTATGATGTTTAATTTAATTAGTTATATGGTGTTG
TTTTCATTCCTCTGTAGGCGACATTTGTACAATAAACAGAAAAGTATAAA
TTCAGAAAGACTGTACGTGTCCATGCAGATGAACAAAAGTAAACCTGTTG
CATTAACATCGGATAATGAAACAGAAATAACCAAGAAAGATTCGAAAATA
AATTTAAAACAAATTTCGGTAAATCGGAAAATTGGTTCAATTAAGTTTGT
TAAAGTTTTACTTGGAGTGAATGTTTTAATGATTACATCCTTTTCTCCAG
CAATTTTGTATAATGCTGATTTTATTAAATTTCCAACATATTCTGTGTAT
TATTTATTCTTTTTGCATTCTCTTGGAGATCCAATTTTATTTTTTTTGAC
TCAGAAAGAACTTAAAAATGGATTGATTTCTATTTTTAAAACTTCTTGAG
TCACAATAGATATATTTACGAAGGAAGATTTATTGAAACATTTTCTTGTA
AAGATTCTTGTAAATTTAG
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protein sequence of SMED30016269-orf-1

>SMED30016269-orf-1 ID=SMED30016269-orf-1|Name=SMED30016269-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=333bp
MNISLGHENGICRKEDTMKGVVDIFFYPIYSLMFILSVLFNSLLLYVVTN
RVKFCHDKRTFHKLFQILIILDLIISLLILPNIIIKGFTKDTDTHWYTDL
FLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVI
IFLSYGVRFILLTINISCSMKKRIDTHGKDIIGISGGVFMMFNLISYMVL
FSFLCRRHLYNKQKSINSERLYVSMQMNKSKPVALTSDNETEITKKDSKI
NLKQISVNRKIGSIKFVKVLLGVNVLMITSFSPAILYNADFIKFPTYSVY
YLFFLHSLGDPILFFLTQKELKNGLISIFKTS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0003001lateral region of the whole animal
PLANA:0003104enteric muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePRINTSPR00237GPCRRHODOPSNcoord: 25..49
score: 22.24
coord: 296..322
score: 16.67
coord: 105..127
score: 24.55
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 12..329
e-value: 1.1E-17
score: 66.0
NoneNo IPR availablePANTHERPTHR24230G-PROTEIN COUPLED RECEPTORcoord: 24..330
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 21..330
NoneNo IPR availableTMHMMTMhelixcoord: 67..89
NoneNo IPR availableTMHMMTMhelixcoord: 182..204
NoneNo IPR availableTMHMMTMhelixcoord: 109..127
NoneNo IPR availableTMHMMTMhelixcoord: 269..287
NoneNo IPR availableTMHMMTMhelixcoord: 140..162
NoneNo IPR availableTMHMMTMhelixcoord: 24..46
NoneNo IPR availableTMHMMTMhelixcoord: 297..316
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 40..314
score: 9.82