SMED30016268

Overview
NameSMED30016268
Smed IDSMED30016268
Length (bp)1629
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30016268 (SMED30016268) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30016268 (SMED30016268) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30016268 (SMED30016268) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30016268 (SMED30016268) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30016268

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
cephalic gangliaSMED30016268h1SMcG0016733 dd_Smed_v4_11543_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30016268h1SMcG0016733 dd_Smed_v4_11543_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016268h1SMcG0016733 dd_Smed_v4_3498_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30016268h1SMcG0016733 dd_Smed_v4_3498_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016268h1SMcG0016733 dd_Smed_v4_3498_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30016268 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000072:77825..101093 -7675
Homology
[Close]
BLAST of SMED30016268 vs. TrEMBL
Match: A0A2G8L3W3 (SCP domain-containing protein OS=Stichopus japonicus OX=307972 GN=BSL78_08259 PE=3 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.233e-6
Identity = 57/237 (24.05%), Postives = 93/237 (39.24%), Query Frame = 2 
Query:  143 SIFTFNEKQFILDSHNWIRAYVAKGVHGLPPTSNMRVMDWNEELEQKSKTY-NKCRGSSFNSYEIKFRANTPYLTIKYKSKEFTNRIIITNLIESIVHQLNSSFYIECMGNILCLVRNQILEPQLSAIGCSVTECEPFTN------ILTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDVLCAKESHCYKTPTKQCSCMITKESCLNHGIFLADKCTCICKPGYKGSDCQDI 832
            S FT  E   ++D HN+ R+         PP +NM  +DW+  L+  ++ + +KC        ++          IKY        ++     E   + L+++    C     C    Q++    ++IGC    C           I+ C Y+    +  F    + G  CS C S    C  ++  + E H      + C C   ++SC N G    + CTC C  GY G+DC  I
Sbjct:   59 SPFTEEEIDQMVDWHNFARSTAD------PPATNMEYIDWDPYLQDIAQRWADKCLWQHGRPSDVVGSYGQNLWAIKYPGVPKAESVVKGWDDEKHDYNLSTN---ACKQGKQCGHYTQVVWSSTNSIGCGKAFCPNLEGAMKNMWIVVCQYSPAGNVVGF-SPYDTGPSCSRCRSGVGQCYENLCRSCERH-----DEHCDC---RQSCNNCGTVDKETCTCQCPQGYHGTDCSSI 277
[Close]
BLAST of SMED30016268 vs. TrEMBL
Match: A0A1I8IW12 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 2.739e-6
Identity = 56/227 (24.67%), Postives = 94/227 (41.41%), Query Frame = 2 
Query:   86 FILGILFSFVNLFSHLSHCSIFTFNEKQFILDSHNWIRAYVAK----GVHGLPPTSNMRVMDWNEELEQKSKTY-NKCRGSSFNSYEIKFRANTPYLTIKYKSKEFTNRIIITNLIESIVHQLNSSFYIECMG---------------NILCLVRNQILEPQLSAIGCSVTEC--EPFTNILTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDVLCAKESHC 700
             +L +L S  +L+  L H    + N K +I+  HN IRA VAK    G    P  +NM+ + W EEL  K++ + + C G   +    +  AN P +           + ++  L  S + +    F  EC G                 +C     ++  +   +GC+  +C  +P    L C Y     +       + G PC+ C S  M CL++ LC+ + +C
Sbjct:    8 VLLALLPSAFSLYGPLEHAIGLSDNAKLYIMCQHNRIRAQVAKGGDTGYKSQPKAANMQALCWCEELVAKAQEHASNCSGPPHDKRSGRKTANFPDV----------GQSLMNTLRSSPIDEAMEGFLEECNGYDFENNYCDTAKAGRAHVCGHYTALVWAETQCLGCAYDQCSGKPHGR-LVCNYGPGGNIEGKKPYVS-GEPCTQCKSGQM-CLDNGLCSDKMNC 221
[Close]
BLAST of SMED30016268 vs. TrEMBL
Match: A0A1I8H0L0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig024285g1 PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 2.941e-6
Identity = 56/226 (24.78%), Postives = 94/226 (41.59%), Query Frame = 2 
Query:   89 ILGILFSFVNLFSHLSHCSIFTFNEKQFILDSHNWIRAYVAK----GVHGLPPTSNMRVMDWNEELEQKSKTY-NKCRGSSFNSYEIKFRANTPYLTIKYKSKEFTNRIIITNLIESIVHQLNSSFYIECMG---------------NILCLVRNQILEPQLSAIGCSVTEC--EPFTNILTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDVLCAKESHC 700
            +L +L S  +L+  L H    + N K +I+  HN IRA VAK    G    P  +NM+ + W EEL  K++ + + C G   +    +  AN P +           + ++  L  S + +    F  EC G                 +C     ++  +   +GC+  +C  +P    L C Y     +       + G PC+ C S  M CL++ LC+ + +C
Sbjct:    9 LLALLPSAFSLYGPLEHAIGLSDNAKLYIMCQHNRIRAQVAKGGDTGYKSQPKAANMQALCWCEELVAKAQEHASNCSGPPHDKRSGRKTANFPDV----------GQSLMNTLRSSPIDEAMEGFLEECNGYDFENNYCDTAKAGRAHVCGHYTALVWAETQCLGCAYDQCSGKPHGR-LVCNYGPGGNIEGKKPYVS-GEPCTQCKSGQM-CLDNGLCSDKMNC 221
[Close]
BLAST of SMED30016268 vs. TrEMBL
Match: A0A267G673 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig024285g3 PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 3.243e-6
Identity = 56/226 (24.78%), Postives = 94/226 (41.59%), Query Frame = 2 
Query:   89 ILGILFSFVNLFSHLSHCSIFTFNEKQFILDSHNWIRAYVAK----GVHGLPPTSNMRVMDWNEELEQKSKTY-NKCRGSSFNSYEIKFRANTPYLTIKYKSKEFTNRIIITNLIESIVHQLNSSFYIECMG---------------NILCLVRNQILEPQLSAIGCSVTEC--EPFTNILTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDVLCAKESHC 700
            +L +L S  +L+  L H    + N K +I+  HN IRA VAK    G    P  +NM+ + W EEL  K++ + + C G   +    +  AN P +           + ++  L  S + +    F  EC G                 +C     ++  +   +GC+  +C  +P    L C Y     +       + G PC+ C S  M CL++ LC+ + +C
Sbjct:    9 LLALLPSAFSLYGPLEHAIGLSDNAKLYIMCQHNRIRAQVAKGGDTGYKSQPKAANMQALCWCEELVAKAQEHASNCSGPPHDKRSGRKTANFPDV----------GQSLMNTLRSSPIDEAMEGFLEECNGYDFENNYCDTAKAGRAHVCGHYTALVWAETQCLGCTYDQCSGKPHGR-LVCNYGPGGNIEGKKPYVS-GEPCTQCKSGQM-CLDNGLCSDKMNC 221
[Close]
BLAST of SMED30016268 vs. TrEMBL
Match: V4B6J8 (SCP domain-containing protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_203268 PE=4 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 9.418e-6
Identity = 60/244 (24.59%), Postives = 96/244 (39.34%), Query Frame = 2 
Query:  158 NEKQFILDSHNWIRAYVAKGVHGLPPTSNMRVMDWNEELEQKSKTYNK-CRGSSFNSYEIKFRANTPYLTIKYKSKEFTNRIIITNLIE-------SIVHQLNSSFYIECMGNILCLVRNQILEPQLSAIGCSVTECEPFTN------ILTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDVLCAKESHCYKTPTKQCSCMITKESCLNHGIFLADKCTCICKPGYKGSDCQDIVFKSE 847
            + K+ I+D HN +R+ V       P  S M  M W++EL + ++ + K C+   F     K RA   Y ++        + +  T  I+       S VH   S       G ++     Q++      +GC   +C P ++         C Y    + + F    ++G PCS+CS    NC   +             K C C    + C N G    D CTC C   Y G+DC  +  K++
Sbjct:   57 SAKKEIVDRHNKLRSEVT------PAASGMVKMVWDDELAKIAEKWAKQCK---FGHDNYKKRATKAYPSVFIGQNGAFDTVTWTEAIDEWYSEKKSFVHGKGSK-----DGGVIGHY-TQVVNDNAVRVGCGRADCPPGSDENFQKTTWFCNYARGQLTSEFPNPYSLGTPCSSCSG---NCDQSL-------------KLCDC--GGKICQNDGTMQYDTCTCKCSALYTGADCTQVNCKAD 267
[Close]
BLAST of SMED30016268 vs. Ensembl Cavefish
Match: tenm4 (teneurin transmembrane protein 4 [Source:NCBI gene;Acc:103031446])

HSP 1 Score: 50.447 bits (119), Expect = 5.217e-6
Identity = 39/133 (29.32%), Postives = 62/133 (46.62%), Query Frame = 2 
Query:  473 ILCLVRNQILEPQ-LSAIGCSVTECEPFTNI---LTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDV-----LCAK-ESHCY------KTPTKQCSCMITKESCLNHGIFLADKCTCICKPGYKGSDC 823
            +LC    Q L+ + +   G   +EC+  TN    +TC+ +   I+ + +   N G+   NC    ++CL+       +C + E HC+         + + SCM   + C  HG FLAD  TC C P + G DC
Sbjct:  670 VLCSGNGQYLKGRCMCHSGWKGSECDVPTNQCIDVTCSGHGTCIVGTCI--CNPGYKGENCEE--VDCLDPTCSGRGVCVRGECHCFVGWGGPGCESPRASCM---DQCSGHGAFLADTGTCSCDPNWTGHDC 795
[Close]
BLAST of SMED30016268 vs. Ensembl Cavefish
Match: tenm4 (teneurin transmembrane protein 4 [Source:NCBI gene;Acc:103031446])

HSP 1 Score: 50.447 bits (119), Expect = 5.300e-6
Identity = 39/133 (29.32%), Postives = 62/133 (46.62%), Query Frame = 2 
Query:  473 ILCLVRNQILEPQ-LSAIGCSVTECEPFTNI---LTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDV-----LCAK-ESHCY------KTPTKQCSCMITKESCLNHGIFLADKCTCICKPGYKGSDC 823
            +LC    Q L+ + +   G   +EC+  TN    +TC+ +   I+ + +   N G+   NC    ++CL+       +C + E HC+         + + SCM   + C  HG FLAD  TC C P + G DC
Sbjct:  622 VLCSGNGQYLKGRCMCHSGWKGSECDVPTNQCIDVTCSGHGTCIVGTCI--CNPGYKGENCEE--VDCLDPTCSGRGVCVRGECHCFVGWGGPGCESPRASCM---DQCSGHGAFLADTGTCSCDPNWTGHDC 747
[Close]
BLAST of SMED30016268 vs. Ensembl Cavefish
Match: tenm4 (teneurin transmembrane protein 4 [Source:NCBI gene;Acc:103031446])

HSP 1 Score: 50.447 bits (119), Expect = 5.336e-6
Identity = 39/133 (29.32%), Postives = 62/133 (46.62%), Query Frame = 2 
Query:  473 ILCLVRNQILEPQ-LSAIGCSVTECEPFTNI---LTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDV-----LCAK-ESHCY------KTPTKQCSCMITKESCLNHGIFLADKCTCICKPGYKGSDC 823
            +LC    Q L+ + +   G   +EC+  TN    +TC+ +   I+ + +   N G+   NC    ++CL+       +C + E HC+         + + SCM   + C  HG FLAD  TC C P + G DC
Sbjct:  571 VLCSGNGQYLKGRCMCHSGWKGSECDVPTNQCIDVTCSGHGTCIVGTCI--CNPGYKGENCEE--VDCLDPTCSGRGVCVRGECHCFVGWGGPGCESPRASCM---DQCSGHGAFLADTGTCSCDPNWTGHDC 696
[Close]
BLAST of SMED30016268 vs. Ensembl Cavefish
Match: tenm4 (teneurin transmembrane protein 4 [Source:NCBI gene;Acc:103031446])

HSP 1 Score: 50.447 bits (119), Expect = 5.341e-6
Identity = 39/133 (29.32%), Postives = 62/133 (46.62%), Query Frame = 2 
Query:  473 ILCLVRNQILEPQ-LSAIGCSVTECEPFTNI---LTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDV-----LCAK-ESHCY------KTPTKQCSCMITKESCLNHGIFLADKCTCICKPGYKGSDC 823
            +LC    Q L+ + +   G   +EC+  TN    +TC+ +   I+ + +   N G+   NC    ++CL+       +C + E HC+         + + SCM   + C  HG FLAD  TC C P + G DC
Sbjct:  604 VLCSGNGQYLKGRCMCHSGWKGSECDVPTNQCIDVTCSGHGTCIVGTCI--CNPGYKGENCEE--VDCLDPTCSGRGVCVRGECHCFVGWGGPGCESPRASCM---DQCSGHGAFLADTGTCSCDPNWTGHDC 729
[Close]
BLAST of SMED30016268 vs. Ensembl Cavefish
Match: tenm4 (teneurin transmembrane protein 4 [Source:NCBI gene;Acc:103031446])

HSP 1 Score: 50.447 bits (119), Expect = 5.346e-6
Identity = 39/133 (29.32%), Postives = 62/133 (46.62%), Query Frame = 2 
Query:  473 ILCLVRNQILEPQ-LSAIGCSVTECEPFTNI---LTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDV-----LCAK-ESHCY------KTPTKQCSCMITKESCLNHGIFLADKCTCICKPGYKGSDC 823
            +LC    Q L+ + +   G   +EC+  TN    +TC+ +   I+ + +   N G+   NC    ++CL+       +C + E HC+         + + SCM   + C  HG FLAD  TC C P + G DC
Sbjct:  637 VLCSGNGQYLKGRCMCHSGWKGSECDVPTNQCIDVTCSGHGTCIVGTCI--CNPGYKGENCEE--VDCLDPTCSGRGVCVRGECHCFVGWGGPGCESPRASCM---DQCSGHGAFLADTGTCSCDPNWTGHDC 762
[Close]
BLAST of SMED30016268 vs. Ensembl Medaka
Match: tenm4 (teneurin transmembrane protein 4 [Source:NCBI gene;Acc:101171508])

HSP 1 Score: 49.6766 bits (117), Expect = 9.897e-6
Identity = 39/133 (29.32%), Postives = 61/133 (45.86%), Query Frame = 2 
Query:  473 ILCLVRNQILEPQ-LSAIGCSVTECEPFTNI---LTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDV-----LCAK-ESHCY------KTPTKQCSCMITKESCLNHGIFLADKCTCICKPGYKGSDC 823
            +LC    Q L+ + +   G   +EC+  TN    +TC+     I+ + +   N G+   NC    ++CL+       +C + E HC+         + + SCM   + C  HG FLAD  TC C P + G DC
Sbjct:  583 VLCSGNGQYLKGRCMCHSGWKGSECDVPTNQCIDVTCSGRGTCIMGTCI--CNPGYKGENCEE--VDCLDPTCSGRGVCVQGECHCFVGWGGSGCESPRASCM---DQCSGHGAFLADTGTCSCDPNWTGHDC 708
[Close]
BLAST of SMED30016268 vs. Ensembl Medaka
Match: tenm4 (teneurin transmembrane protein 4 [Source:NCBI gene;Acc:101171508])

HSP 1 Score: 49.6766 bits (117), Expect = 9.912e-6
Identity = 39/133 (29.32%), Postives = 61/133 (45.86%), Query Frame = 2 
Query:  473 ILCLVRNQILEPQ-LSAIGCSVTECEPFTNI---LTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDV-----LCAK-ESHCY------KTPTKQCSCMITKESCLNHGIFLADKCTCICKPGYKGSDC 823
            +LC    Q L+ + +   G   +EC+  TN    +TC+     I+ + +   N G+   NC    ++CL+       +C + E HC+         + + SCM   + C  HG FLAD  TC C P + G DC
Sbjct:  641 VLCSGNGQYLKGRCMCHSGWKGSECDVPTNQCIDVTCSGRGTCIMGTCI--CNPGYKGENCEE--VDCLDPTCSGRGVCVQGECHCFVGWGGSGCESPRASCM---DQCSGHGAFLADTGTCSCDPNWTGHDC 766
[Close]
BLAST of SMED30016268 vs. Ensembl Medaka
Match: tenm4 (teneurin transmembrane protein 4 [Source:NCBI gene;Acc:101171508])

HSP 1 Score: 49.6766 bits (117), Expect = 9.924e-6
Identity = 39/133 (29.32%), Postives = 61/133 (45.86%), Query Frame = 2 
Query:  473 ILCLVRNQILEPQ-LSAIGCSVTECEPFTNI---LTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDV-----LCAK-ESHCY------KTPTKQCSCMITKESCLNHGIFLADKCTCICKPGYKGSDC 823
            +LC    Q L+ + +   G   +EC+  TN    +TC+     I+ + +   N G+   NC    ++CL+       +C + E HC+         + + SCM   + C  HG FLAD  TC C P + G DC
Sbjct:  690 VLCSGNGQYLKGRCMCHSGWKGSECDVPTNQCIDVTCSGRGTCIMGTCI--CNPGYKGENCEE--VDCLDPTCSGRGVCVQGECHCFVGWGGSGCESPRASCM---DQCSGHGAFLADTGTCSCDPNWTGHDC 815
[Close]
BLAST of SMED30016268 vs. Planmine SMEST
Match: SMESG000077815.1 (SMESG000077815.1)

HSP 1 Score: 650.973 bits (1678), Expect = 0.000e+0
Identity = 329/330 (99.70%), Postives = 330/330 (100.00%), Query Frame = 2 
Query:   77 MWKFILGILFSFVNLFSHLSHCSIFTFNEKQFILDSHNWIRAYVAKGVHGLPPTSNMRVMDWNEELEQKSKTYNKCRGSSFNSYEIKFRANTPYLTIKYKSKEFTNRIIITNLIESIVHQLNSSFYIECMGNILCLVRNQILEPQLSAIGCSVTECEPFTNILTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDVLCAKESHCYKTPTKQCSCMITKESCLNHGIFLADKCTCICKPGYKGSDCQDIVFKSEVDRTRFLINTENKPNPNIETKIRCNDQLWECPNWAAMGLCFLKTVRXXXXXXXXXXXXDEQLISHFKSILMLR 1066
            MWKFILGILFSFVNLFSHLSHCSIFTFNEKQFILDSHNWIRAYVAKGVHGLPPTSNMRVMDWNEELEQKSKTYNKCRGSSFNSYEIKFRANTPYLTIKYKSKEFTNRIIITNLIESIVHQLNSSFYIECMGNILCLVRNQILEPQLSAIGCSVTECEPFTNILTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDVLCAKESHCYKTPTKQCSCMITKESCLNHGIFLADKCTCICKPGYKGSDCQDIVFKSEVDRTRFLINTENKPNPNIETKIRCNDQLWECPNWAAMGLCFLKTVRSSCSESCEKCESDEQLISHFKSILML+
Sbjct:    1 MWKFILGILFSFVNLFSHLSHCSIFTFNEKQFILDSHNWIRAYVAKGVHGLPPTSNMRVMDWNEELEQKSKTYNKCRGSSFNSYEIKFRANTPYLTIKYKSKEFTNRIIITNLIESIVHQLNSSFYIECMGNILCLVRNQILEPQLSAIGCSVTECEPFTNILTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDVLCAKESHCYKTPTKQCSCMITKESCLNHGIFLADKCTCICKPGYKGSDCQDIVFKSEVDRTRFLINTENKPNPNIETKIRCNDQLWECPNWAAMGLCFLKTVRSSCSESCEKCESDEQLISHFKSILMLK 330

HSP 2 Score: 363.999 bits (933), Expect = 0.000e+0
Identity = 181/189 (95.77%), Postives = 182/189 (96.30%), Query Frame = 3 
Query: 1065 DAGSECVDKRSECQMWADKNYCYIKAVQITCPRVCALCPDPPEPEKFVCKEDPPRAATCKQVIGNYLCYSPDMKEVCPVTCGNTKCGTPLPTTTLKDPCTEPESHLKPCSDFKKVGYCSIKEVMEYCPVTCEMDKCRTDPELCKDLKEFCSTEKIKKKCKQTC----VPTSALKSTFLPTTEQTAEPSV 1619
            DAGSECVDKRSECQMWADKNYCYIKAVQITCPRVCALCPDPPEPEKFVCKEDPPRAATCKQVIGNYLCYSPDMKEVCPVTCGNTKCGTPLPTTTLKDPCTEPESHLKPCSDFKKVGYCSIKEVMEYCPVTCEMDKCRTDPELCKDLKEFCSTEKIKKKCKQTC    VPTSALKSTFLPTTEQT   S+
Sbjct:  331 DAGSECVDKRSECQMWADKNYCYIKAVQITCPRVCALCPDPPEPEKFVCKEDPPRAATCKQVIGNYLCYSPDMKEVCPVTCGNTKCGTPLPTTTLKDPCTEPESHLKPCSDFKKVGYCSIKEVMEYCPVTCEMDKCRTDPELCKDLKEFCSTEKIKKKCKQTCGTSVVPTSALKSTFLPTTEQTGSISI 519
[Close]
BLAST of SMED30016268 vs. Planmine SMEST
Match: SMESG000066348.1 (SMESG000066348.1)

HSP 1 Score: 100.908 bits (250), Expect = 2.245e-22
Identity = 81/269 (30.11%), Postives = 126/269 (46.84%), Query Frame = 2 
Query:   89 ILGILFSFVNLFS-HLSHCSIFTFNEKQFILDSHNWIRAYVAKGVHGLPP-TSNMRVMDWNEELEQKSKTYNK-CRGSSFNSYEIKFRANTPYLTIKYKSKEFTNRIIITNLIESIVH---QLNSSFYIECMGN-ILCLVRNQILEPQLSAIGCSVTECEPFTNI-LTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDVLCAKESHC-YKTPTKQCSCMITKESCLNHGIFLADKCTCICKPGYKGSDCQD--IVFKSEVDRTR 862
            +L  +F  + LFS +   C     +EKQ  LD HNW RA   +  +G  P  ++M  +DW+E+L  KSK   K C  SS  S+ +K +  +    + + SK   +   +++ +++  +   + +++   +C  N ++C    QIL  +    GC+   CE    +   C YN K   +   +    G  CS C      CL+  LCA +S C  K     C C I  +SC  HGI   D C C CKPGY G  C++  I  + +  R R
Sbjct:    5 LLSCIFLSLILFSVNYPKCLCLRKSEKQLFLDLHNWYRAKFTRNTYGDQPVAADMNALDWDEKLAWKSKNVVKLCLNSSEESHFVKHQFGSDIGIVSF-SKTINDSQSLSDFLKTAFNIWIKYSTNLENDCDKNSVICDNIKQILYSRGENFGCAKNFCENDKTVHFACLYNYKFNKSRIFQH---GDSCSLCQQNQPFCLDGSLCATKSQCKRKNVNSHCRCAINYKSCHPHGILKLDSCNCRCKPGYYGPTCKEQCIDLQEDCQRMR 269

HSP 2 Score: 62.003 bits (149), Expect = 5.651e-10
Identity = 29/84 (34.52%), Postives = 46/84 (54.76%), Query Frame = 3 
Query: 1071 GSECVDKRSECQMWADKNYCYIKAVQITCPRVCALCPDPPEPEKFVCKEDPPRAATCKQVIGNYLCYSPDMKEVCPVTCGNTKC 1322
             + CVD    C  W D+NYC+++ +Q  C   C +C +  +    VC E   + + C +V    LC +PDM+++CP+TC   KC
Sbjct:  420 SAGCVDNNPNCPNWKDQNYCHMENIQQQCRLSCKMCLNSGK--NIVCDESSQKTSACLRVQNLNLCTAPDMQKICPITCNYKKC 501
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BLAST of SMED30016268 vs. Planmine SMEST
Match: SMESG000035186.1 (SMESG000035186.1)

HSP 1 Score: 80.4925 bits (197), Expect = 1.828e-15
Identity = 79/328 (24.09%), Postives = 139/328 (42.38%), Query Frame = 2 
Query:  161 EKQFILDSHNWIRAYVAKG-VHGLPPTSNMRVMDWNEELEQKSKT-YNKC-RGSSFNSYEIK-FRANTPYLTIKYKSKEFTNRIIITNLIESIVH-----QLNSSFYIECMGNILCLVRNQILEPQLSAIGCSVTECEPFTNILTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDVLCAKESHCYKTP-TKQCSCMITKESCLNHGIFLADKCTCICKPGYKGSDCQ------------------DIVFKSEV---------------------DRTRFLINTENKPNPNIETKIR----CNDQLWECPNWAAMGLCFLKTVR 985
            +KQ ILD HNW RA +++G +   P +S+M V++W++ L + +     KC +    N YEI  F+ +   L     ++ FTN     N  +  VH      +N  +         C     ++   +  +GC   +C    +++ C Y++    +   +    G  C++C      C++  LCA + HC  +  +K C C++  + C  HG + + KC C C+PG+ G +C+                  D+  KS++                     +     +NT   P  N E + +    C D   EC  WA+ G C L++V+
Sbjct:   29 DKQLILDLHNWYRAQISRGDIFQQPESSDMTVVEWDDRLAEIALIRAQKCLKEKKVNDYEISSFQHD--LLGRMAINEYFTNDT--DNYTKKAVHSWFKNHINFEYENGTCTKSDCQSYKNMIFSNVFVLGCEAYKCVNSISVI-CIYSNSERTDKGEDLYKSGLSCTSCPQDYSFCMDGSLCASKFHCRDSKISKTCRCLVLPKLCTKHGDYDSSKCRCRCQPGWTGLNCEKPCHNSSPLCENRKWACTDVTHKSQMFKACPQTCKWCQNNPNSIPIYNENEIKVNTIYDPT-NFELEDQSDGICKDNKMECGAWASKGFCNLESVK 350
[Close]
BLAST of SMED30016268 vs. Planmine SMEST
Match: SMESG000035186.1 (SMESG000035186.1)

HSP 1 Score: 80.1073 bits (196), Expect = 2.100e-15
Identity = 79/328 (24.09%), Postives = 139/328 (42.38%), Query Frame = 2 
Query:  161 EKQFILDSHNWIRAYVAKG-VHGLPPTSNMRVMDWNEELEQKSKT-YNKC-RGSSFNSYEIK-FRANTPYLTIKYKSKEFTNRIIITNLIESIVH-----QLNSSFYIECMGNILCLVRNQILEPQLSAIGCSVTECEPFTNILTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDVLCAKESHCYKTP-TKQCSCMITKESCLNHGIFLADKCTCICKPGYKGSDCQ------------------DIVFKSEV---------------------DRTRFLINTENKPNPNIETKIR----CNDQLWECPNWAAMGLCFLKTVR 985
            +KQ ILD HNW RA +++G +   P +S+M V++W++ L + +     KC +    N YEI  F+ +   L     ++ FTN     N  +  VH      +N  +         C     ++   +  +GC   +C    +++ C Y++    +   +    G  C++C      C++  LCA + HC  +  +K C C++  + C  HG + + KC C C+PG+ G +C+                  D+  KS++                     +     +NT   P  N E + +    C D   EC  WA+ G C L++V+
Sbjct:   29 DKQLILDLHNWYRAQISRGDIFQQPESSDMTVVEWDDRLAEIALIRAQKCLKEKKVNDYEISSFQHD--LLGRMAINEYFTNDT--DNYTKKAVHSWFKNHINFEYENGTCTKSDCQSYKNMIFSNVFVLGCEAYKCVNSISVI-CIYSNSERTDKGEDLYKSGLSCTSCPQDYSFCMDGSLCASKFHCRDSKISKTCRCLVLPKLCTKHGDYDSSKCRCRCQPGWTGLNCEKPCHNSSPLCENRKWACTDVTHKSQMFKACPQTCKWCQNNPNSIPIYNENEIKVNTIYDPT-NFELEDQSDGICKDNKMECGAWASKGFCNLESVK 350
[Close]
BLAST of SMED30016268 vs. Planmine SMEST
Match: SMESG000035186.1 (SMESG000035186.1)

HSP 1 Score: 79.7221 bits (195), Expect = 2.660e-15
Identity = 79/328 (24.09%), Postives = 139/328 (42.38%), Query Frame = 2 
Query:  161 EKQFILDSHNWIRAYVAKG-VHGLPPTSNMRVMDWNEELEQKSKT-YNKC-RGSSFNSYEIK-FRANTPYLTIKYKSKEFTNRIIITNLIESIVH-----QLNSSFYIECMGNILCLVRNQILEPQLSAIGCSVTECEPFTNILTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDVLCAKESHCYKTP-TKQCSCMITKESCLNHGIFLADKCTCICKPGYKGSDCQ------------------DIVFKSEV---------------------DRTRFLINTENKPNPNIETKIR----CNDQLWECPNWAAMGLCFLKTVR 985
            +KQ ILD HNW RA +++G +   P +S+M V++W++ L + +     KC +    N YEI  F+ +   L     ++ FTN     N  +  VH      +N  +         C     ++   +  +GC   +C    +++ C Y++    +   +    G  C++C      C++  LCA + HC  +  +K C C++  + C  HG + + KC C C+PG+ G +C+                  D+  KS++                     +     +NT   P  N E + +    C D   EC  WA+ G C L++V+
Sbjct:    2 DKQLILDLHNWYRAQISRGDIFQQPESSDMTVVEWDDRLAEIALIRAQKCLKEKKVNDYEISSFQHD--LLGRMAINEYFTNDT--DNYTKKAVHSWFKNHINFEYENGTCTKSDCQSYKNMIFSNVFVLGCEAYKCVNSISVI-CIYSNSERTDKGEDLYKSGLSCTSCPQDYSFCMDGSLCASKFHCRDSKISKTCRCLVLPKLCTKHGDYDSSKCRCRCQPGWTGLNCEKPCHNSSPLCENRKWACTDVTHKSQMFKACPQTCKWCQNNPNSIPIYNENEIKVNTIYDPT-NFELEDQSDGICKDNKMECGAWASKGFCNLESVK 323
The following BLAST results are available for this feature:
BLAST of SMED30016268 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016268 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016268 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016268 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016268 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016268 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016268 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016268 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2G8L3W32.233e-624.05SCP domain-containing protein OS=Stichopus japonic... [more]
A0A1I8IW122.739e-624.67Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8H0L02.941e-624.78Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267G6733.243e-624.78Uncharacterized protein OS=Macrostomum lignano OX=... [more]
V4B6J89.418e-624.59SCP domain-containing protein OS=Lottia gigantea O... [more]
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BLAST of SMED30016268 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tenm45.217e-629.32teneurin transmembrane protein 4 [Source:NCBI gene... [more]
tenm45.300e-629.32teneurin transmembrane protein 4 [Source:NCBI gene... [more]
tenm45.336e-629.32teneurin transmembrane protein 4 [Source:NCBI gene... [more]
tenm45.341e-629.32teneurin transmembrane protein 4 [Source:NCBI gene... [more]
tenm45.346e-629.32teneurin transmembrane protein 4 [Source:NCBI gene... [more]
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BLAST of SMED30016268 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016268 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016268 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016268 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
tenm49.897e-629.32teneurin transmembrane protein 4 [Source:NCBI gene... [more]
tenm49.912e-629.32teneurin transmembrane protein 4 [Source:NCBI gene... [more]
tenm49.924e-629.32teneurin transmembrane protein 4 [Source:NCBI gene... [more]
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BLAST of SMED30016268 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000077815.10.000e+099.70SMESG000077815.1[more]
SMESG000066348.12.245e-2230.11SMESG000066348.1[more]
SMESG000035186.11.828e-1524.09SMESG000035186.1[more]
SMESG000035186.12.100e-1524.09SMESG000035186.1[more]
SMESG000035186.12.660e-1524.09SMESG000035186.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016268 ID=SMED30016268|Name=SMED30016268|organism=Schmidtea mediterranea sexual|type=transcript|length=1629bp
CCTGATTCACTGATAATTTGCATTTTTCTTTTTCACCCATCATTAATCTT
TCTACTTATCTTGTTTTAGACAACACATGTGGAAATTCATACTTGGGATT
TTGTTTTCTTTTGTAAATCTTTTCTCCCATTTGTCTCATTGTTCTATCTT
CACATTCAATGAAAAACAGTTCATTCTTGATTCCCACAACTGGATACGAG
CCTATGTGGCTAAAGGTGTTCATGGATTACCACCGACTTCCAACATGCGA
GTCATGGATTGGAATGAAGAACTTGAACAAAAATCAAAAACCTATAATAA
ATGCCGAGGTAGCTCGTTCAATTCTTATGAAATTAAATTCAGAGCAAATA
CGCCATATTTAACAATTAAATACAAATCCAAGGAGTTCACAAATCGGATT
ATAATTACAAATTTAATTGAAAGTATTGTCCATCAACTTAACAGTTCATT
CTATATTGAATGCATGGGAAATATACTTTGTCTAGTTAGAAATCAGATCC
TTGAGCCGCAATTGTCTGCTATTGGCTGTTCTGTCACAGAGTGCGAACCA
TTCACAAACATTCTGACTTGTACTTACAACTCAAAAGCTATTTTAAACTC
TTTTCTGGAATCGACAAACATTGGATTTCCATGTTCTAATTGTTCTAGTA
GAAATATGAATTGTTTGAACGATGTTTTATGTGCAAAGGAATCTCACTGC
TATAAGACTCCAACAAAACAATGTAGTTGTATGATTACCAAAGAATCCTG
TTTAAACCATGGGATATTTCTGGCGGATAAATGCACATGTATCTGCAAAC
CAGGCTACAAAGGAAGTGATTGTCAAGATATCGTTTTCAAATCTGAGGTT
GACAGGACAAGGTTTTTAATAAACACAGAAAATAAACCTAATCCAAATAT
TGAGACGAAAATTCGATGCAATGATCAACTTTGGGAATGTCCAAATTGGG
CGGCCATGGGTCTTTGCTTTCTAAAGACTGTGCGAAGTTCTTGTTCCGAA
TCATGTGAAAAATGTGAATCAGATGAACAACTTATTAGTCATTTTAAATC
TATTTTAATGCTAAGATGCAGGCAGTGAATGCGTTGATAAAAGAAGTGAA
TGTCAAATGTGGGCAGACAAGAATTATTGTTATATAAAAGCGGTTCAAAT
AACTTGTCCCAGGGTTTGCGCACTTTGCCCTGATCCTCCGGAACCCGAAA
AATTTGTATGCAAAGAAGATCCACCGAGAGCTGCTACATGTAAACAGGTA
ATTGGAAATTACCTTTGTTATTCTCCAGACATGAAAGAAGTTTGCCCAGT
GACATGTGGTAATACCAAATGTGGAACCCCTTTGCCCACTACGACATTAA
AAGATCCATGCACTGAACCAGAATCTCACTTAAAACCCTGTAGTGATTTC
AAAAAGGTAGGATATTGTTCAATAAAAGAAGTAATGGAATATTGTCCTGT
CACGTGCGAAATGGACAAATGCAGAACTGATCCAGAATTATGTAAAGACT
TAAAGGAATTTTGCTCCACTGAAAAGATTAAAAAAAAATGCAAACAAACT
TGTGTTCCAACGTCGGCTTTAAAATCAACTTTTTTACCAACAACAGAACA
AACAGCCGAGCCATCAGTGTGTCCAGATA
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protein sequence of SMED30016268-orf-1

>SMED30016268-orf-1 ID=SMED30016268-orf-1|Name=SMED30016268-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=334bp
MWKFILGILFSFVNLFSHLSHCSIFTFNEKQFILDSHNWIRAYVAKGVHG
LPPTSNMRVMDWNEELEQKSKTYNKCRGSSFNSYEIKFRANTPYLTIKYK
SKEFTNRIIITNLIESIVHQLNSSFYIECMGNILCLVRNQILEPQLSAIG
CSVTECEPFTNILTCTYNSKAILNSFLESTNIGFPCSNCSSRNMNCLNDV
LCAKESHCYKTPTKQCSCMITKESCLNHGIFLADKCTCICKPGYKGSDCQ
DIVFKSEVDRTRFLINTENKPNPNIETKIRCNDQLWECPNWAAMGLCFLK
TVRSSCSESCEKCESDEQLISHFKSILMLRCRQ*
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protein sequence of SMED30016268-orf-2

>SMED30016268-orf-2 ID=SMED30016268-orf-2|Name=SMED30016268-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=174bp
MWADKNYCYIKAVQITCPRVCALCPDPPEPEKFVCKEDPPRAATCKQVIG
NYLCYSPDMKEVCPVTCGNTKCGTPLPTTTLKDPCTEPESHLKPCSDFKK
VGYCSIKEVMEYCPVTCEMDKCRTDPELCKDLKEFCSTEKIKKKCKQTCV
PTSALKSTFLPTTEQTAEPSVCPD
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0002056medial muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR035940CAP_sf
IPR003582ShKT_dom
IPR014044CAP_domain
IPR013032EGF-like_CS
Vocabulary: molecular function
TermDefinition
GO:0030246carbohydrate binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014044CAP domainSMARTSM00198SCP_3coord: 28..171
e-value: 2.7E-4
score: -16.4
IPR014044CAP domainPFAMPF00188CAPcoord: 34..167
e-value: 3.9E-7
score: 30.9
IPR035940CAP superfamilyGENE3DG3DSA:3.40.33.10coord: 15..218
e-value: 5.9E-18
score: 67.3
IPR035940CAP superfamilySUPERFAMILYSSF55797PR-1-likecoord: 15..168
IPR003582ShKT domainPROSITEPS51670SHKTcoord: 281..313
score: 8.097
NoneNo IPR availableCDDcd05380CAP_eukcoord: 30..168
e-value: 6.33109E-11
score: 57.8495
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..22
score: 0.635