SMED30016208

Overview


Name	SMED30016208
Smed ID	SMED30016208
Length (bp)	589

Neoblast Clusters

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30016208

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Expressed In	Reference Transcript	Published Transcript	Transcriptome	Publication
nervous system	SMED30016208	dd_Smed_v4_99720_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30016208	dd_Smed_v4_99720_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
non-ciliated neuron	SMED30016208	dd_Smed_v4_99720_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Alignments

SMED30016208 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.020844:8425..9013 +	2945
v31.028300:566..1396 +	2881
v31.022225:10297..10871 -	2225
v31.019537:6686..7176 +	1555

Homology

[Close]

BLAST of SMED30016208 vs. Ensembl Human
Match: PGBD1 (piggyBac transposable element derived 1 [Source:HGNC Symbol;Acc:HGNC:19398])

HSP 1 Score: 134.42 bits (337), Expect = 5.708e-36
Identity = 71/177 (40.11%), Postives = 111/177 (62.71%), Query Frame = -3

Query:   45 KYSKVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTFVYN-STHEDMLLCKW 572
            K++K+RPL+  +N  FL YAP+EE +  D++M   ++ D   QF+ GKPI  GYK + GT   GY+ WFEPYQ  S +  D+  +LGLG ++++ ++DVLL+     Y + L F +FFTS+ L+  LK +G+ ATG I ENR   C L+  + ++K  R  F +    + +++LC+W
Sbjct:  518 KFTKLRPLIKQMNKNFLLYAPLEEYYCFDKSMCECFDSD---QFLNGKPIRIGYKIWCGTTTQGYLVWFEPYQEESTMKVDEDPDLGLGGNLVMNFADVLLER--GQYPYHLCFDSFFTSVKLLSALKKKGVRATGTIRENRTEKCPLMNVEHMKKMKRGYFDFRIEENNEIILCRW 689

[Close]

BLAST of SMED30016208 vs. Ensembl Human
Match: ERCC6 (ERCC excision repair 6, chromatin remodeling factor [Source:HGNC Symbol;Acc:HGNC:3438])

HSP 1 Score: 129.413 bits (324), Expect = 2.113e-34
Identity = 73/176 (41.48%), Postives = 107/176 (60.80%), Query Frame = -3

Query:   45 KYSKVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTFVYNSTHEDMLLCKW 572
            K+SK+RPL+  LN + + + P E   S DE MVPY+ R   KQFI GKPI  GYK++ G   LGY+ WF+PYQG  K  + K++E G+GAS++L +S+ L +     Y F+  F NFFTSI L+ +L + G  ATG + ++      L +   L+K+ R TF Y    +  ++C+W
Sbjct:  242 KFSKLRPLISKLNERCMKFVPNETYFSFDEFMVPYFGRHGCKQFIRGKPIRFGYKFWCGATCLGYICWFQPYQG--KNPNTKHEEYGVGASLVLQFSEALTEAHPGQYHFV--FNNFFTSIALLDKLSSMGHQATGTVRKDHIDRVPLESDVALKKKERGTFDYRIDGKGNIVCRW 413

[Close]

BLAST of SMED30016208 vs. Ensembl Human
Match: ERCC6 (ERCC excision repair 6, chromatin remodeling factor [Source:HGNC Symbol;Acc:HGNC:3438])

HSP 1 Score: 128.642 bits (322), Expect = 6.695e-34
Identity = 73/176 (41.48%), Postives = 107/176 (60.80%), Query Frame = -3

Query:   45 KYSKVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTFVYNSTHEDMLLCKW 572
            K+SK+RPL+  LN + + + P E   S DE MVPY+ R   KQFI GKPI  GYK++ G   LGY+ WF+PYQG  K  + K++E G+GAS++L +S+ L +     Y F+  F NFFTSI L+ +L + G  ATG + ++      L +   L+K+ R TF Y    +  ++C+W
Sbjct:  710 KFSKLRPLISKLNERCMKFVPNETYFSFDEFMVPYFGRHGCKQFIRGKPIRFGYKFWCGATCLGYICWFQPYQG--KNPNTKHEEYGVGASLVLQFSEALTEAHPGQYHFV--FNNFFTSIALLDKLSSMGHQATGTVRKDHIDRVPLESDVALKKKERGTFDYRIDGKGNIVCRW 881

[Close]

Query:   45 KYSKVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTFVYNSTHEDMLLCKW 572
            K+SK+RPL+  LN + + + P E   S DE MVPY+ R   KQFI GKPI  GYK++ G   LGY+ WF+PYQG  K  + K++E G+GAS++L +S+ L +     Y F+  F NFFTSI L+ +L + G  ATG + ++      L +   L+K+ R TF Y    +  ++C+W
Sbjct:  710 KFSKLRPLISKLNERCMKFVPNETYFSFDEFMVPYFGRHGCKQFIRGKPIRFGYKFWCGATCLGYICWFQPYQG--KNPNTKHEEYGVGASLVLQFSEALTEAHPGQYHFV--FNNFFTSIALLDKLSSMGHQATGTVRKDHIDRVPLESDVALKKKERGTFDYRIDGKGNIVCRW 881

[Close]

BLAST of SMED30016208 vs. Ensembl Human
Match: PGBD2 (piggyBac transposable element derived 2 [Source:HGNC Symbol;Acc:HGNC:19399])

HSP 1 Score: 120.168 bits (300), Expect = 3.901e-31
Identity = 65/177 (36.72%), Postives = 101/177 (57.06%), Query Frame = -3

Query:   45 KYSKVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTFVYN-STHEDMLLCKW 572
            +++KVRPL+  +N  F  +AP+EE +S  E+M  Y+     KQ   GKP+  GYK + GT   GY+ WFEP QG   + +   + L LG S+++ + D L +     Y   ++F   FTS+ LM  L+ +G+ ATG + E R   C L  PK+L+K  R +F Y     E++++C+W
Sbjct:  241 RFAKVRPLIIRMNCNFQKHAPLEEFYSFGESMCEYFGHRGSKQLHRGKPVRLGYKIWCGTTSRGYLVWFEPSQGT--LFTKPDRSLDLGGSMVIKFVDALQERGFLPYH--IFFDKVFTSVKLMSILRKKGVKATGTVREYRTERCPLKDPKELKKMKRGSFDYKVDESEEIIVCRW 413

[Close]

BLAST of SMED30016208 vs. Ensembl Zebrafish
Match: CABZ01069162.1 (pep scaffold:GRCz11:KN149950.1:74:5493:1 gene:ENSDARG00000115161.1 transcript:ENSDART00000191202.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CABZ01069162.1)

HSP 1 Score: 59.6918 bits (143), Expect = 2.398e-10
Identity = 51/181 (28.18%), Postives = 83/181 (45.86%), Query Frame = -3

Query:   45 TAFFKYSKVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYV-GTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTFVYNSTHEDMLLCKW 584
             A+ +  K++PL D +      +    +N S+DE MV    R   KQ++  KP   GYK +V   ++ GY + F  Y+G      +     GL    ++   D   K+ G  Y   LY  NF+TS  L  +L ++ I A G I  NR +G   +A  +L ++     +     +D+L  +W
Sbjct:  331 AAYDRLGKIKPLYDNIRDACRAFYHPGQNISIDERMVASKARSGLKQYMKNKPTKWGYKLFVLADSQCGYTSEFFVYEGKRMSSPN-----GLSYESVMALIDE--KYLGTGYK--LYVDNFYTSPKLFRDLLSKRIYACGTIRPNR-IGFPKMATNKLPQDAPRGSMRWIREDDLLFVEW 501

[Close]

BLAST of SMED30016208 vs. Ensembl Zebrafish
Match: si:ch211-255f4.7 (si:ch211-255f4.7 [Source:ZFIN;Acc:ZDB-GENE-050208-628])

HSP 1 Score: 49.6766 bits (117), Expect = 7.680e-7
Identity = 55/181 (30.39%), Postives = 75/181 (41.44%), Query Frame = -3

Query:   45 KYSKVRPLLD-CLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAP--KQLQKEVRCTFVYNSTHEDMLLC--KW 572
            K+ KVRP LD  L G      P  +   + E M+P+      +Q++  KP   G K +V     G V  FE YQG   +I    +  GLG   +++       H G      +Y   FFT+I     +  + +  TG I  NR  G     P  K +    R T    ST ED  LC  KW
Sbjct:  267 KFWKVRPFLDRILQGCLSLNRP--DCACIGEQMIPFTGACPYRQYLPMKPNPIGVKNFVCATADGIVLDFELYQGTGSLIGKVEEPEGLGLGCLVIERLCQTLHRGTK----VYCDRFFTTIKGAQRMMEKELYMTGTIMNNRLAGAKHKLPSDKAMISMGRGTSSEVST-EDGKLCVVKW 440

[Close]

BLAST of SMED30016208 vs. Ensembl Zebrafish
Match: pgbd4 (piggyBac transposable element derived 4 [Source:ZFIN;Acc:ZDB-GENE-091204-417])

HSP 1 Score: 48.521 bits (114), Expect = 1.736e-6
Identity = 52/176 (29.55%), Postives = 81/176 (46.02%), Query Frame = -3

Query:   51 KVRPL-LDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNW-FEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPK---QLQKEVRCTFVYNSTHEDMLLC 563
            K++PL  D  +    ++ P  +N S+DE MV    R    Q+I  +P   GYK +V    L    W F  Y+G  K+ +++ K +   + + L+   VL    G  Y   L+  NF+TS  L  +L ++ I A G I  NR       AP+   +  +E    FV      D+L+C
Sbjct:  271 KIKPLYQDIRDACKASFHP-HQNISIDERMVASKARHGLIQYI-SEPTKWGYKLFVLADSLCAYTWDFFIYEG--KVKTEQRKGISYDSVMALIDERVL----GTGYK--LFVDNFYTSPTLFRDLLSKKIGACGTIRPNRINHTETEAPRGNIRWFREDGLLFVEWKDKRDVLMC 436

[Close]

BLAST of SMED30016208 vs. Ensembl Zebrafish
Match: pgbd4 (piggyBac transposable element derived 4 [Source:ZFIN;Acc:ZDB-GENE-091204-417])

HSP 1 Score: 48.521 bits (114), Expect = 1.754e-6
Identity = 52/176 (29.55%), Postives = 81/176 (46.02%), Query Frame = -3

Query:   51 KVRPL-LDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNW-FEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPK---QLQKEVRCTFVYNSTHEDMLLC 563
            K++PL  D  +    ++ P  +N S+DE MV    R    Q+I  +P   GYK +V    L    W F  Y+G  K+ +++ K +   + + L+   VL    G  Y   L+  NF+TS  L  +L ++ I A G I  NR       AP+   +  +E    FV      D+L+C
Sbjct:  271 KIKPLYQDIRDACKASFHP-HQNISIDERMVASKARHGLIQYI-SEPTKWGYKLFVLADSLCAYTWDFFIYEG--KVKTEQRKGISYDSVMALIDERVL----GTGYK--LFVDNFYTSPTLFRDLLSKKIGACGTIRPNRINHTETEAPRGNIRWFREDGLLFVEWKDKRDVLMC 436

[Close]

BLAST of SMED30016208 vs. Ensembl Xenopus
Match: ENSXETT00000026678.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:4:88624292:88625603:1 gene:ENSXETG00000013495.1 transcript:ENSXETT00000026678.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.4546 bits (132), Expect = 1.045e-8
Identity = 45/154 (29.22%), Postives = 74/154 (48.05%), Query Frame = -3

Query:  105 KVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYY-VGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQK 563
            K+RPL+D L+ +F       +N  VDE+++ +  R   +Q+I  K    G K+Y +  +  GY ++F  Y+G    +        L  S  +V+ +++    G  Y   LY  NF+TSIPL   L +    A G +  NR      L  K+L++
Sbjct:  167 KLRPLIDSLSQRFAEVYTPSQNVCVDESLLLFKGRLKFRQYIPSKRSRYGMKFYKLCESSTGYTSYFMLYEGKDTNLDPPGCPTDLTTSGKIVW-ELITPLLGRGYH--LYVDNFYTSIPLFRALNSLDTPACGTVNRNRKGLPRELLDKKLKR 317

[Close]

BLAST of SMED30016208 vs. Ensembl Xenopus
Match: ENSXETT00000019041.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:7:47398238:47399566:-1 gene:ENSXETG00000007669.1 transcript:ENSXETT00000019041.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.0694 bits (131), Expect = 1.168e-8
Identity = 45/154 (29.22%), Postives = 74/154 (48.05%), Query Frame = -3

Query:  105 KVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYY-VGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQK 563
            K+RPL+D L+ +F       +N  VDE+++ +  R   +Q+I  K    G K+Y +  +  GY ++F  Y+G    +        L  S  +V+ +++    G  Y   LY  NF+TSIPL   L +    A G +  NR      L  K+L++
Sbjct:   34 KLRPLIDSLSQRFAEVYTPSQNVCVDESLLLFKGRLKFRQYIPSKRSRYGMKFYKLCESSTGYTSYFMLYEGKDTNLDPPGCPTDLTTSGKIVW-ELITPLLGRGYH--LYVDNFYTSIPLFRALNSLDTPACGTVNRNRKGLPRELLDKKLKR 184

[Close]

BLAST of SMED30016208 vs. Ensembl Xenopus
Match: ENSXETT00000019443.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:2:30938263:30940062:1 gene:ENSXETG00000007374.1 transcript:ENSXETT00000019443.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.0694 bits (131), Expect = 1.298e-8
Identity = 45/154 (29.22%), Postives = 74/154 (48.05%), Query Frame = -3

Query:  105 KVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYY-VGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQK 563
            K+RPL+D L+ +F       +N  VDE+++ +  R   +Q+I  K    G K+Y +  +  GY ++F  Y+G    +        L  S  +V+ +++    G  Y   LY  NF+TSIPL   L +    A G +  NR      L  K+L++
Sbjct:  240 KLRPLIDSLSQRFAEVYTPSQNVCVDESLLLFKGRLKFRQYIPSKRSRYGMKFYKLCESSTGYTSYFMLYEGKDTNLDPPGCPTDLTTSGKIVW-ELITPLLGRGYH--LYVDNFYTSIPLFRALNSLDTPACGTVNRNRKGLPRELLDKKLKR 390

[Close]

BLAST of SMED30016208 vs. Ensembl Xenopus
Match: ENSXETT00000036375.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:7:83635951:83637829:-1 gene:ENSXETG00000016372.1 transcript:ENSXETT00000036375.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.0694 bits (131), Expect = 1.364e-8
Identity = 48/159 (30.19%), Postives = 75/159 (47.17%), Query Frame = -3

Query:   93 KVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYY-VGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRN-LGCSLLAPKQLQKEVRC 563
            K+RPL+D L+ +F       +N  VDE+++ +  R   +Q+I  K    G K+Y +  +  GY ++F  Y+G    +        L  S  +V+ +++    G  Y   LY  NF+TSIPL   L +    A G +  NR  L   LL  K  + EV  
Sbjct:  240 KLRPLIDSLSQRFAEVYTPSQNVCVDESLLLFKGRLKFRQYIPSKRSRYGMKFYKLCESSTGYTSYFMLYEGKDTNLDPPGCPTDLTTSGKIVW-ELITPLLGRGYH--LYVDNFYTSIPLFRALNSLDTPACGTVNRNRKGLPRELLDKKLKRGEVHA 395

[Close]

BLAST of SMED30016208 vs. Ensembl Xenopus
Match: ENSXETT00000026903.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:4:20337945:20339225:1 gene:ENSXETG00000013290.1 transcript:ENSXETT00000026903.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.0694 bits (131), Expect = 1.370e-8
Identity = 45/154 (29.22%), Postives = 74/154 (48.05%), Query Frame = -3

Query:  105 KVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYY-VGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQK 563
            K+RPL+D L+ +F       +N  VDE+++ +  R   +Q+I  K    G K+Y +  +  GY ++F  Y+G    +        L  S  +V+ +++    G  Y   LY  NF+TSIPL   L +    A G +  NR      L  K+L++
Sbjct:  240 KLRPLIDSLSQRFAEVYTPSQNVCVDESLLLFKGRLKFRQYIPSKRSRYGMKFYKLCESSTGYTSYFMLYEGKDTNLDPPGCPTDLTTSGKIVW-ELITPLLGRGYH--LYVDNFYTSIPLFRALNSLDTPACGTVNRNRKGLPRELLDKKLKR 390

[Close]

BLAST of SMED30016208 vs. UniProt/SwissProt
Match: sp|Q96JS3|PGBD1_HUMAN (PiggyBac transposable element-derived protein 1 OS=Homo sapiens OX=9606 GN=PGBD1 PE=1 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 2.741e-35
Identity = 71/177 (40.11%), Postives = 111/177 (62.71%), Query Frame = -3

Query:   45 KYSKVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTFVYN-STHEDMLLCKW 572
            K++K+RPL+  +N  FL YAP+EE +  D++M   ++ D   QF+ GKPI  GYK + GT   GY+ WFEPYQ  S +  D+  +LGLG ++++ ++DVLL+     Y + L F +FFTS+ L+  LK +G+ ATG I ENR   C L+  + ++K  R  F +    + +++LC+W
Sbjct:  518 KFTKLRPLIKQMNKNFLLYAPLEEYYCFDKSMCECFDSD---QFLNGKPIRIGYKIWCGTTTQGYLVWFEPYQEESTMKVDEDPDLGLGGNLVMNFADVLLER--GQYPYHLCFDSFFTSVKLLSALKKKGVRATGTIRENRTEKCPLMNVEHMKKMKRGYFDFRIEENNEIILCRW 689

[Close]

BLAST of SMED30016208 vs. UniProt/SwissProt
Match: sp|Q8N328|PGBD3_HUMAN (PiggyBac transposable element-derived protein 3 OS=Homo sapiens OX=9606 GN=PGBD3 PE=1 SV=3)

HSP 1 Score: 129.413 bits (324), Expect = 1.015e-33
Identity = 73/176 (41.48%), Postives = 107/176 (60.80%), Query Frame = -3

Query:   45 KYSKVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTFVYNSTHEDMLLCKW 572
            K+SK+RPL+  LN + + + P E   S DE MVPY+ R   KQFI GKPI  GYK++ G   LGY+ WF+PYQG  K  + K++E G+GAS++L +S+ L +     Y F+  F NFFTSI L+ +L + G  ATG + ++      L +   L+K+ R TF Y    +  ++C+W
Sbjct:  242 KFSKLRPLISKLNERCMKFVPNETYFSFDEFMVPYFGRHGCKQFIRGKPIRFGYKFWCGATCLGYICWFQPYQG--KNPNTKHEEYGVGASLVLQFSEALTEAHPGQYHFV--FNNFFTSIALLDKLSSMGHQATGTVRKDHIDRVPLESDVALKKKERGTFDYRIDGKGNIVCRW 413

[Close]

BLAST of SMED30016208 vs. UniProt/SwissProt
Match: sp|P0DP91|ERPG3_HUMAN (Chimeric ERCC6-PGBD3 protein OS=Homo sapiens OX=9606 GN=ERCC6 PE=1 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 3.215e-33
Identity = 73/176 (41.48%), Postives = 107/176 (60.80%), Query Frame = -3

Query:   45 KYSKVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTFVYNSTHEDMLLCKW 572
            K+SK+RPL+  LN + + + P E   S DE MVPY+ R   KQFI GKPI  GYK++ G   LGY+ WF+PYQG  K  + K++E G+GAS++L +S+ L +     Y F+  F NFFTSI L+ +L + G  ATG + ++      L +   L+K+ R TF Y    +  ++C+W
Sbjct:  710 KFSKLRPLISKLNERCMKFVPNETYFSFDEFMVPYFGRHGCKQFIRGKPIRFGYKFWCGATCLGYICWFQPYQG--KNPNTKHEEYGVGASLVLQFSEALTEAHPGQYHFV--FNNFFTSIALLDKLSSMGHQATGTVRKDHIDRVPLESDVALKKKERGTFDYRIDGKGNIVCRW 881

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BLAST of SMED30016208 vs. UniProt/SwissProt
Match: sp|Q6P3X8|PGBD2_HUMAN (PiggyBac transposable element-derived protein 2 OS=Homo sapiens OX=9606 GN=PGBD2 PE=2 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 1.874e-30
Identity = 65/177 (36.72%), Postives = 101/177 (57.06%), Query Frame = -3

Query:   45 KYSKVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTFVYN-STHEDMLLCKW 572
            +++KVRPL+  +N  F  +AP+EE +S  E+M  Y+     KQ   GKP+  GYK + GT   GY+ WFEP QG   + +   + L LG S+++ + D L +     Y   ++F   FTS+ LM  L+ +G+ ATG + E R   C L  PK+L+K  R +F Y     E++++C+W
Sbjct:  241 RFAKVRPLIIRMNCNFQKHAPLEEFYSFGESMCEYFGHRGSKQLHRGKPVRLGYKIWCGTTSRGYLVWFEPSQGT--LFTKPDRSLDLGGSMVIKFVDALQERGFLPYH--IFFDKVFTSVKLMSILRKKGVKATGTVREYRTERCPLKDPKELKKMKRGSFDYKVDESEEIIVCRW 413

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BLAST of SMED30016208 vs. TrEMBL
Match: A0A4C1YGA8 (PiggyBac transposable element-derived protein 3 OS=Eumeta variegata OX=151549 GN=PGBD3 PE=4 SV=1)

HSP 1 Score: 175.637 bits (444), Expect = 8.415e-51
Identity = 87/177 (49.15%), Postives = 119/177 (67.23%), Query Frame = -3

Query:   45 KYSKVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTFVYNSTHEDM-LLCKW 572
            KY+KVRPL+  LN KFL ++P+EE++SVDE M+PYY R   KQ I GKPI  G+K +VG  RLGYV W EPYQGA+ + +  YKELGLGASV+L + DVL+   G +  + + F NFFT  PL+ E+  +G+  TG + ENR   C L+  K L+K+ R    Y +TH++  ++ KW
Sbjct:   41 KYTKVRPLVTLLNKKFLEFSPLEEHYSVDEAMIPYYGRHGCKQHIKGKPIRYGFKAWVGATRLGYVLWMEPYQGATTMCNPIYKELGLGASVVLTFCDVLISR-GFDLPYHVVFDNFFTGTPLLEEITKKGLRCTGTVRENRTSSCPLITSKLLKKKERGAVDYRTTHDNTFIIAKW 216

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BLAST of SMED30016208 vs. TrEMBL
Match: A0A4C1ZQ73 (PiggyBac transposable element-derived protein 3 OS=Eumeta variegata OX=151549 GN=PGBD3 PE=4 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 2.414e-50
Identity = 87/177 (49.15%), Postives = 119/177 (67.23%), Query Frame = -3

Query:   45 KYSKVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTFVYNSTHED-MLLCKW 572
            KY+KVRPL+  LN KFL ++P+EE++SVDE M+PYY R   KQ I GKPI  G+K +VG  RLGYV W EPYQGA+ + +  YKELGLGASV+L + DVL+   G +  + + F NFFT  PL+ E+  +G+  TG + ENR   C L+  K L+K+ R    Y +TH++  ++ KW
Sbjct:   24 KYTKVRPLVTLLNKKFLEFSPLEEHYSVDEAMIPYYGRHGCKQHIKGKPIRYGFKAWVGATRLGYVLWMEPYQGATTMCNPIYKELGLGASVVLTFCDVLISR-GFDLPYHVVFDNFFTGTPLLKEITKKGLRCTGTVRENRTSSCPLITSKLLKKKERGAVDYRTTHDNTFIIAKW 199

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BLAST of SMED30016208 vs. TrEMBL
Match: A0A4C1WZA1 (PiggyBac transposable element-derived protein 3 OS=Eumeta variegata OX=151549 GN=PGBD3 PE=4 SV=1)

HSP 1 Score: 174.866 bits (442), Expect = 1.014e-49
Identity = 87/177 (49.15%), Postives = 119/177 (67.23%), Query Frame = -3

Query:   45 KYSKVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTFVYNSTHEDM-LLCKW 572
            KY+KVRPL+  LN KFL ++P+EE++SVDE M+PYY R   KQ I GKPI  G+K +VG  RLGYV W EPYQGA+ + +  YKELGLGASV+L + DVL+   G +  + + F NFFT  PL+ E+  +G+  TG + ENR   C L+  K L+K+ R    Y +TH++  ++ KW
Sbjct:   24 KYTKVRPLVTLLNKKFLEFSPLEEHYSVDEAMIPYYGRHGCKQHIKGKPIRYGFKAWVGATRLGYVLWMEPYQGATTLCNPIYKELGLGASVVLTFCDVLISR-GFDLPYHVVFDNFFTGTPLLEEITKKGLRCTGTVRENRTSSCPLITSKLLKKKERGAVDYRTTHDNTFIIAKW 199

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BLAST of SMED30016208 vs. TrEMBL
Match: A0A1B6J1D9 (DDE_Tnp_1_7 domain-containing protein (Fragment) OS=Homalodisca liturata OX=320908 GN=g.2739 PE=4 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 1.582e-49
Identity = 88/177 (49.72%), Postives = 119/177 (67.23%), Query Frame = -3

Query:   45 KYSKVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTFVYNST-HEDMLLCKW 572
            KY+KVRPL D LN  F   AP  E+HS+DE+MVPY+ R S KQFI GKPI  GYK +VG +  GYV W +PYQG S+ I   YK+ GLGASV+L Y DVL K+   ++++ L+F NFFTS+PL+ ELK R I ATG + E+R   C L   K ++K+ R T  Y +  ++ +++ +W
Sbjct:   28 KYAKVRPLFDMLNESFFENAPHVEHHSIDESMVPYFGRHSLKQFIRGKPIRYGYKIWVGASTQGYVIWKDPYQGKSQTIDPAYKQFGLGASVVLNYCDVLRKN--GDFSYFLFFDNFFTSLPLLQELKKRNIRATGTMREDRIGDCPLEDAKTIKKKERGTLQYKTEPNKKLIVARW 202

[Close]

BLAST of SMED30016208 vs. TrEMBL
Match: A0A1B6JW45 (DDE_Tnp_1_7 domain-containing protein (Fragment) OS=Homalodisca liturata OX=320908 GN=g.2740 PE=4 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 1.877e-49
Identity = 88/177 (49.72%), Postives = 119/177 (67.23%), Query Frame = -3

Query:   45 KYSKVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTFVYNST-HEDMLLCKW 572
            KY+KVRPL D LN  F   AP  E+HS+DE+MVPY+ R S KQFI GKPI  GYK +VG +  GYV W +PYQG S+ I   YK+ GLGASV+L Y DVL K+   ++++ L+F NFFTS+PL+ ELK R I ATG + E+R   C L   K ++K+ R T  Y +  ++ +++ +W
Sbjct:   34 KYAKVRPLFDMLNESFFENAPHVEHHSIDESMVPYFGRHSLKQFIRGKPIRYGYKIWVGASTQGYVIWKDPYQGKSQTIDPAYKQFGLGASVVLNYCDVLRKN--GDFSYFLFFDNFFTSLPLLQELKKRNIRATGTMREDRIGDCPLEDAKTIKKKERGTLQYKTEPNKKLIVARW 208

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BLAST of SMED30016208 vs. Ensembl Cavefish
Match: ENSAMXT00000029839.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000180.1:82354:86597:-1 gene:ENSAMXG00000035772.1 transcript:ENSAMXT00000029839.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 91.6633 bits (226), Expect = 2.253e-21
Identity = 58/179 (32.40%), Postives = 95/179 (53.07%), Query Frame = -3

Query:   45 KYSKVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVIL-VYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCS--LLAPKQLQKEVRCTFVYNSTHEDMLLCKW 572
            ++ K+RP+LD L   F +    +E  SVDE M+PY  R S KQ++  KP   G K +V     GY+  FEPYQG +    +   +LG+   V++ +  D+  K+        ++F NFF +IPL+  L+++GI  TG    NR  G    L   KQ+++E R +    +  +++ + +W
Sbjct:  199 RFVKIRPVLDALKETFQSALDPDEFQSVDEMMIPYKGRLSIKQYVPKKPKPWGIKVWVRAGSSGYMYNFEPYQGPAGGRGE-ISQLGMAGDVVMRLCQDIQDKNHK------VFFDNFFCTIPLLQALEHQGIYGTGTCRSNRLHGAQEKLKKEKQMKQEGRGSVSVVTNAQNITITRW 370

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BLAST of SMED30016208 vs. Ensembl Cavefish
Match: ENSAMXT00000044689.1 (piggyBac transposable element-derived protein 3-like [Source:NCBI gene;Acc:111192949])

HSP 1 Score: 86.2705 bits (212), Expect = 1.231e-19
Identity = 59/159 (37.11%), Postives = 83/159 (52.20%), Query Frame = -3

Query:   87 KVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTF 563
            K+RPL+D L  KF    P E+  S+DE MVP+  R   KQ+I  KP   GYK +V     G V+ F+ Y G S       +++G   +V+L  + V+      +   LLYF N+F+S+ L   L  RGI A G + +NR   CS      L+K+ R TF
Sbjct:  228 KIRPLIDSLLPKF-QALPQEQMMSIDEQMVPFKGRSVLKQYIPKKPYKWGYKIFVLCDTKGLVHSFDVYAGKSDPPPGS-QDIGASGNVVLKLAQVI----EGSVNHLLYFDNWFSSLNLFVALAKRGIPALGTVRQNRLQDCSFSKDDTLKKKGRGTF 380

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BLAST of SMED30016208 vs. Ensembl Cavefish
Match: ENSAMXT00000030708.1 (pep primary_assembly:Astyanax_mexicanus-2.0:14:30675304:30677836:1 gene:ENSAMXG00000043231.1 transcript:ENSAMXT00000030708.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 83.9593 bits (206), Expect = 7.713e-19
Identity = 53/147 (36.05%), Postives = 79/147 (53.74%), Query Frame = -3

Query:  135 KYSKVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVIL-VYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGC 572
            ++ K+RP+LD L   F +    EE  SVDE M+PY  R S KQ++  KP   G K +V     GY+  FEPYQG +     +  +LG+   V++ +  D+  K+        ++F NFF +IPL+  L+++GI  TG    NR  G 
Sbjct:  182 RFVKIRPVLDALKETFQSALDPEEFQSVDEMMIPYKGRLSIKQYVPKKPKPWGIKVWVRAGSSGYMYNFEPYQGPAG-GRGEISQLGMAGDVVMRLCQDIQDKNHK------VFFDNFFCTIPLLQALEHQGIYGTGTCRSNRLHGA 321

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BLAST of SMED30016208 vs. Ensembl Cavefish
Match: ENSAMXT00000049307.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000190.1:48620:52067:1 gene:ENSAMXG00000030374.1 transcript:ENSAMXT00000049307.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 80.8777 bits (198), Expect = 1.114e-17
Identity = 60/157 (38.22%), Postives = 83/157 (52.87%), Query Frame = -3

Query:   96 KVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKY-KELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVR 563
            KVRPL D +  + L   P+EEN  VDE MVP+    S KQFI GKP   G K Y    + G    F  +QG +  +S ++ K+LGLGA V+   S  + +    N+   LYF N+FTS  L+  L  R I+A G    +R     L + K++ K+ R
Sbjct:  142 KVRPLYDVIRSRCLEL-PLEENLCVDEQMVPFRGTLSVKQFIKGKPHPWGVKIYFMCGKSGMAYDFLLHQGTTMELSQQHRKQLGLGAGVVYHLSQRITE---ANHK--LYFDNYFTSYNLLELLAERKIHAAGTARVSRFARPPLQSDKEMAKKPR 292

[Close]

BLAST of SMED30016208 vs. Ensembl Cavefish
Match: ENSAMXT00000041934.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000117.1:399080:403449:1 gene:ENSAMXG00000029930.1 transcript:ENSAMXT00000041934.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 79.337 bits (194), Expect = 4.084e-17
Identity = 57/173 (32.95%), Postives = 81/173 (46.82%), Query Frame = -3

Query:   45 KVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTFVYNSTHEDMLLCKW 563
            KVRP+   LN  +    P  E  +VDE+M+ YY     KQFI GKP   GYK +   +  GY++  EPY G   ++      LG G +V+L  ++      GC +    Y  N FTS+ L+ E+  RG  + G I +NR          +  K  R T     T  + LL +W
Sbjct:  164 KVRPIFTELNASY-KVMPYSEWLAVDESMIRYYGHHGCKQFIRGKPFRFGYKIWSLASHKGYMHHMEPYGGRFTLLPQ--TRLGQGPTVVLGLAEQGEVPKGCKF----YHDNLFTSLSLLDEMTKRGYGSCGTIRDNRLFDVPFTPKNEFNKLPRGT-TQVLTEGEKLLVRW 328

[Close]

BLAST of SMED30016208 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001816.1 (pep scaffold:Pmarinus_7.0:GL492440:267714:269431:-1 gene:ENSPMAG00000001650.1 transcript:ENSPMAT00000001816.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 46.9802 bits (110), Expect = 8.596e-7
Identity = 43/165 (26.06%), Postives = 69/165 (41.82%), Query Frame = -3

Query:   96 KVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGT-ARLGYVNWFEPYQGASKII------SDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAP--KQLQKEVR 563
            K+RPLLD L G F  +     +  +DE +  + +R      +   PI CG K ++ + A  GYV+    + G   I+        + K   LG    +  ++ L    G ++   +YFG  F S  L+ +   RG             GC L+ P  +   KE+R
Sbjct:  144 KIRPLLDHLLGAFSRHFVPFRDMRLDEVLSKFKDRHDALPCVPANPIRCGVKVFICSCASTGYVHSLLVHAGGHDIVVAGGLWRRRNKSFPLGYRAAMRLTERL---HGVHHH--VYFGKLFGSARLLSDFLQRG-----------TYGCGLVRPNSRGFPKELR 292

[Close]

BLAST of SMED30016208 vs. Ensembl Medaka
Match: ENSORLT00000038522.1 (pep primary_assembly:ASM223467v1:10:9345195:9347118:-1 gene:ENSORLG00000027882.1 transcript:ENSORLT00000038522.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 82.0333 bits (201), Expect = 4.821e-18
Identity = 53/159 (33.33%), Postives = 83/159 (52.20%), Query Frame = -3

Query:   87 KVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTF 563
            K+RPL+  L  K L   P+ E  +VDE MVP+  R+  KQ++  KP   GYK  V     G  + FE Y G   +   +  ++G   +V+L  +  + KH        L+F N+FTS+PL+  L  +GI+  G +  NR  G S++   +L++  R +F
Sbjct:  207 KIRPLVTHLTSK-LQTIPMGEKLAVDEQMVPFKGRNRLKQYLPAKPKKWGYKILVLAGSDGVPHNFEIYTGRV-VQPPELPDVGASGNVVLRLAQPIPKH----QKHKLFFDNWFTSVPLILTLAQQGIHCAGTVRSNRLPGVSMMCDAELKRTGRGSF 359

[Close]

BLAST of SMED30016208 vs. Ensembl Medaka
Match: ENSORLT00000000917.2 (piggyBac transposable element-derived protein 2-like [Source:NCBI gene;Acc:111946443])

HSP 1 Score: 81.2629 bits (199), Expect = 9.346e-18
Identity = 53/159 (33.33%), Postives = 82/159 (51.57%), Query Frame = -3

Query:   87 KVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTF 563
            K+RPL+  L  K L   P+ E   VDE MVP+  R+  KQ++  KP   GYK  V     G  + FE Y G   +   +  ++G   +V+L  +  + KH        L+F N+FTS+PL+  L  +GI+  G +  NR  G S++   +L++  R +F
Sbjct:  207 KIRPLVTHLTSK-LQTIPMGEKLDVDEQMVPFKGRNRLKQYLPAKPKKWGYKILVLAGSDGVPHNFEIYTGRV-VQPPELPDVGASGNVVLRLAQPIPKH----QKHKLFFDNWFTSVPLILTLAQQGIHCPGTVCSNRLPGVSMMCDPELKRTGRGSF 359

[Close]

BLAST of SMED30016208 vs. Ensembl Medaka
Match: ENSORLT00000047092.1 (piggyBac transposable element-derived protein 2-like [Source:NCBI gene;Acc:111948922])

HSP 1 Score: 80.4925 bits (197), Expect = 1.547e-17
Identity = 52/159 (32.70%), Postives = 83/159 (52.20%), Query Frame = -3

Query:   87 KVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTF 563
            K+RPL+  L  K L   P+ E  +VDE MVP+  R+  KQ++  KP   GYK  V     G  + FE Y G   +   +  ++G   +V+L  +  + KH        L+F N++TS+PL+  L  +GI+  G +  NR  G S++   +L++  R +F
Sbjct:  207 KIRPLVTHLTSK-LQTIPMGEKLAVDEQMVPFKGRNRLKQYLPAKPKKWGYKILVLAGSDGVQHNFEIYTGRV-VQPPELPDVGASGNVVLRLAQPIPKH----QKHKLFFDNWYTSVPLILTLAQQGIHCAGTVRSNRLPGVSMMCDAELKRTGRGSF 359

[Close]

BLAST of SMED30016208 vs. Ensembl Medaka
Match: ENSORLT00000036570.1 (pep primary_assembly:ASM223467v1:15:4424179:4426102:1 gene:ENSORLG00000027498.1 transcript:ENSORLT00000036570.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 78.1814 bits (191), Expect = 8.463e-17
Identity = 52/159 (32.70%), Postives = 82/159 (51.57%), Query Frame = -3

Query:   87 KVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTF 563
            K+RPL+  L  K L   P+ E  +VDE MV +  R+  KQ++  KP   GYK  V     G  + FE Y G   +   +  ++G   +V+L  +  + KH        L+F N+FTS+PL+  L  +GI+  G +  NR  G S++   +L++  R +F
Sbjct:  207 KIRPLVTHLTSK-LQTIPMGEKLAVDEQMVTFKGRNRLKQYLPAKPKKWGYKILVLAGSDGVPHNFEIYTGRV-VQPPELPDVGASGNVVLHLAQPIPKH----QKHKLFFDNWFTSVPLILTLAQQGIHCAGTVRSNRLPGVSMMCDAELKRTGRGSF 359

[Close]

BLAST of SMED30016208 vs. Ensembl Medaka
Match: ENSORLT00000042135.1 (pep primary_assembly:ASM223467v1:1:6921929:6923917:1 gene:ENSORLG00000030303.1 transcript:ENSORLT00000042135.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 78.1814 bits (191), Expect = 1.095e-16
Identity = 53/159 (33.33%), Postives = 82/159 (51.57%), Query Frame = -3

Query:   87 KVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTF 563
            K+RPL+  L  K L   P+ E  +VDE MVP+  R+  KQ++  KP    YK  V     G  + FE Y G   +   +  ++G   +V+L  +  + KH        L+F N+FTS+PL+  L  +GI+  G +  NR  G SL+   +L++  R +F
Sbjct:  207 KIRPLVTHLTSK-LQTIPMGEKLAVDEQMVPFKGRNRLKQYLPAKPKKWVYKILVLAGCDGVPHNFEIYTGRV-VQPPELPDVGASGNVVLRLAQPIPKH----QKHKLFFDNWFTSVPLILTLAQQGIHCAGTVRSNRLPGVSLMCDAELKRTGRGSF 359

[Close]

BLAST of SMED30016208 vs. Planmine SMEST
Match: SMESG000070388.1 (SMESG000070388.1)

HSP 1 Score: 299.671 bits (766), Expect = 1.928e-104
Identity = 142/145 (97.93%), Postives = 144/145 (99.31%), Query Frame = -3

Query:   45 MVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTFVYNSTHEDMLLCKW 479
            MVPYYERDSEKQFIWGKPIGCGYK+YVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI EN+NLGCSLLAPKQLQKEVRCTFVYNSTHEDMLLCKW
Sbjct:    1 MVPYYERDSEKQFIWGKPIGCGYKFYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKIRENQNLGCSLLAPKQLQKEVRCTFVYNSTHEDMLLCKW 145

[Close]

BLAST of SMED30016208 vs. Planmine SMEST
Match: SMESG000025761.1 (SMESG000025761.1)

HSP 1 Score: 62.3882 bits (150), Expect = 3.495e-12
Identity = 28/76 (36.84%), Postives = 50/76 (65.79%), Query Frame = -3

Query:  345 KYSKVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGAS 572
            + +K+R L+D L  KF+ +  ++E  ++DE+M+PY+ +   K FI GKPI  GYK +   +++GY++ F+ Y G +
Sbjct:   25 RVAKIRTLIDHLQKKFIEHRSLQERLAIDESMIPYFGKHYGKSFIRGKPIRFGYKMWEICSKVGYLHSFDLYLGKN 100

[Close]

BLAST of SMED30016208 vs. Planmine SMEST
Match: SMESG000039958.1 (SMESG000039958.1)

HSP 1 Score: 61.2326 bits (147), Expect = 6.736e-12
Identity = 45/150 (30.00%), Postives = 70/150 (46.67%), Query Frame = -3

Query:   45 SVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILVYSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*ENRNLGCSLLAPKQLQKEVRCTFVYNSTHEDMLLCKW 494
            SVDE +V +Y R    QFI  KPI  G K ++  +  GY N F      +     +   + LG  V+L ++ +L KH       +L+F NFF+S  L  +LK     A G   EN+ + C L    +L +     F +   ++ + + KW
Sbjct:    3 SVDEQIVKFYARHPLNQFISNKPIRFGLKSWLLCSSFGYCNLFNLCCWKN---DGEKTNVPLGLKVVLHFAKLLTKH----AQHILFFDNFFSSHRLFVDLKEMNTPAIGTFRENQLMKCPLKTENELNRRGSVDFQFYERNQ-IQVVKW 144

[Close]

BLAST of SMED30016208 vs. Planmine SMEST
Match: SMESG000071608.1 (SMESG000071608.1)

HSP 1 Score: 45.8246 bits (107), Expect = 7.117e-6
Identity = 32/92 (34.78%), Postives = 45/92 (48.91%), Query Frame = -3

Query:  297 KYSKVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFIWGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASV 572
            + +K++PLLD L  K+       ENH +DET+VP+  R   +Q+I  K    G K +      GY    + Y G S+I     K  GL A V
Sbjct:  225 RLAKIKPLLDLLEAKYKKVFVPGENHVIDETLVPWRGRLVFRQYIPNKAHKYGIKLFKLCTSEGYTWASKMYSGKSEIYD---KNSGLAAKV 313

The following BLAST results are available for this feature:

BLAST of SMED30016208 vs. Ensembl Human
BLAST of SMED30016208 vs. Ensembl Celegans
BLAST of SMED30016208 vs. Ensembl Fly
BLAST of SMED30016208 vs. Ensembl Zebrafish
BLAST of SMED30016208 vs. Ensembl Xenopus
BLAST of SMED30016208 vs. Ensembl Mouse
BLAST of SMED30016208 vs. UniProt/SwissProt
BLAST of SMED30016208 vs. TrEMBL
BLAST of SMED30016208 vs. Ensembl Cavefish
BLAST of SMED30016208 vs. Ensembl Sea Lamprey
BLAST of SMED30016208 vs. Ensembl Yeast
BLAST of SMED30016208 vs. Ensembl Nematostella
BLAST of SMED30016208 vs. Ensembl Medaka
BLAST of SMED30016208 vs. Planmine SMEST

BLAST of SMED30016208 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
PGBD1	5.708e-36	40.11	piggyBac transposable element derived 1 [Source:HG...	[more]
ERCC6	2.113e-34	41.48	ERCC excision repair 6, chromatin remodeling facto...	[more]
ERCC6	6.695e-34	41.48	ERCC excision repair 6, chromatin remodeling facto...	[more]
ERCC6	6.695e-34	41.48	ERCC excision repair 6, chromatin remodeling facto...	[more]
PGBD2	3.901e-31	36.72	piggyBac transposable element derived 2 [Source:HG...	[more]

BLAST of SMED30016208 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30016208 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30016208 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4


Match Name	E-value	Identity	Description
CABZ01069162.1	2.398e-10	28.18	pep scaffold:GRCz11:KN149950.1:74:5493:1 gene:ENSD...	[more]
si:ch211-255f4.7	7.680e-7	30.39	si:ch211-255f4.7 [Source:ZFIN;Acc:ZDB-GENE-050208-...	[more]
pgbd4	1.736e-6	29.55	piggyBac transposable element derived 4 [Source:ZF...	[more]
pgbd4	1.754e-6	29.55	piggyBac transposable element derived 4 [Source:ZF...	[more]

BLAST of SMED30016208 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSXETT00000026678.1	1.045e-8	29.22	pep primary_assembly:Xenopus_tropicalis_v9.1:4:886...	[more]
ENSXETT00000019041.1	1.168e-8	29.22	pep primary_assembly:Xenopus_tropicalis_v9.1:7:473...	[more]
ENSXETT00000019443.1	1.298e-8	29.22	pep primary_assembly:Xenopus_tropicalis_v9.1:2:309...	[more]
ENSXETT00000036375.1	1.364e-8	30.19	pep primary_assembly:Xenopus_tropicalis_v9.1:7:836...	[more]
ENSXETT00000026903.1	1.370e-8	29.22	pep primary_assembly:Xenopus_tropicalis_v9.1:4:203...	[more]

BLAST of SMED30016208 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30016208 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4


Match Name	E-value	Identity	Description
sp\|Q96JS3\|PGBD1_HUMAN	2.741e-35	40.11	PiggyBac transposable element-derived protein 1 OS...	[more]
sp\|Q8N328\|PGBD3_HUMAN	1.015e-33	41.48	PiggyBac transposable element-derived protein 3 OS...	[more]
sp\|P0DP91\|ERPG3_HUMAN	3.215e-33	41.48	Chimeric ERCC6-PGBD3 protein OS=Homo sapiens OX=96...	[more]
sp\|Q6P3X8\|PGBD2_HUMAN	1.874e-30	36.72	PiggyBac transposable element-derived protein 2 OS...	[more]

BLAST of SMED30016208 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A4C1YGA8	8.415e-51	49.15	PiggyBac transposable element-derived protein 3 OS...	[more]
A0A4C1ZQ73	2.414e-50	49.15	PiggyBac transposable element-derived protein 3 OS...	[more]
A0A4C1WZA1	1.014e-49	49.15	PiggyBac transposable element-derived protein 3 OS...	[more]
A0A1B6J1D9	1.582e-49	49.72	DDE_Tnp_1_7 domain-containing protein (Fragment) O...	[more]
A0A1B6JW45	1.877e-49	49.72	DDE_Tnp_1_7 domain-containing protein (Fragment) O...	[more]

BLAST of SMED30016208 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSAMXT00000029839.1	2.253e-21	32.40	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]
ENSAMXT00000044689.1	1.231e-19	37.11	piggyBac transposable element-derived protein 3-li...	[more]
ENSAMXT00000030708.1	7.713e-19	36.05	pep primary_assembly:Astyanax_mexicanus-2.0:14:306...	[more]
ENSAMXT00000049307.1	1.114e-17	38.22	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]
ENSAMXT00000041934.1	4.084e-17	32.95	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]

BLAST of SMED30016208 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSPMAT00000001816.1	8.596e-7	26.06	pep scaffold:Pmarinus_7.0:GL492440:267714:269431:-...	[more]

BLAST of SMED30016208 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30016208 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30016208 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSORLT00000038522.1	4.821e-18	33.33	pep primary_assembly:ASM223467v1:10:9345195:934711...	[more]
ENSORLT00000000917.2	9.346e-18	33.33	piggyBac transposable element-derived protein 2-li...	[more]
ENSORLT00000047092.1	1.547e-17	32.70	piggyBac transposable element-derived protein 2-li...	[more]
ENSORLT00000036570.1	8.463e-17	32.70	pep primary_assembly:ASM223467v1:15:4424179:442610...	[more]
ENSORLT00000042135.1	1.095e-16	33.33	pep primary_assembly:ASM223467v1:1:6921929:6923917...	[more]

BLAST of SMED30016208 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4


Match Name	E-value	Identity	Description
SMESG000070388.1	1.928e-104	97.93	SMESG000070388.1	[more]
SMESG000025761.1	3.495e-12	36.84	SMESG000025761.1	[more]
SMESG000039958.1	6.736e-12	30.00	SMESG000039958.1	[more]
SMESG000071608.1	7.117e-6	34.78	SMESG000071608.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30016208 ID=SMED30016208|Name=SMED30016208|organism=Schmidtea mediterranea sexual|type=transcript|length=589bp
ATGGAATTTGCATTCGAGCTGATAGCCACAACATTATTGTCATTCCATTT
GCATAGCAACATATCTTCATGTGTAGAATTATAAACAAAGGTGCATCTTA
CCTCTTTTTGTAGTTGTTTAGGGGCTAGTAATGAGCATCCCAAATTTCGA
TTCTCTCAAATTTTGCCAGTTGCGTTTATGCCTCTATTTTTAAGTTCATG
CATTAAAGGAATAGAAGTAAAAAAGTTACCAAAATAGAGAAGAAATGTAT
AATTACATCCAAAATGTTTCAATAACACATCCGAATATACTAAAATTACG
CTGGCACCTAATCCCAGTTCCTTATATTTGTCTGAAATAATTTTAGACGC
ACCTTGATATGGTTCAAACCAATTGACGTATCCCAAGCGTGCGGTTCCAA
CATAATACTTATAACCACATCCAATTGGTTTCCCCCAAATAAACTGCTTT
TCACTATCGCGTTCATAATATGGAACCATAGTTTCATCAACACTGTGATT
CTCTTCAACAGGCGCGTAATTCAAAAACTTTCCATTCAGACAATCCAAGA
GCGGTCTTACTTTTGAATATTTGAAAAAGGCTGTGCCGA

protein sequence of SMED30016208-orf-1

>SMED30016208-orf-1 ID=SMED30016208-orf-1|Name=SMED30016208-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=144bp
GTAFFKYSKVRPLLDCLNGKFLNYAPVEENHSVDETMVPYYERDSEKQFI
WGKPIGCGYKYYVGTARLGYVNWFEPYQGASKIISDKYKELGLGASVILV
YSDVLLKHFGCNYTFLLYFGNFFTSIPLMHELKNRGINATGKI*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **INTERPRO**
Term	Definition
IPR029526	PGBD

Main menu

SMED30016208

Zeng et. al., 2018

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Owlery

blazegraph-runner