SMED30016186

Overview
NameSMED30016186
Smed IDSMED30016186
Length (bp)1116
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30016186 (SMED30016186) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30016186 (SMED30016186) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30016186 (SMED30016186) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30016186 (SMED30016186) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30016186

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 15

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30016186h1SMcG0006639 SmedASXL_017381SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30016186h1SMcG0006639 SmedASXL_017382SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
pharynxSMED30016186h1SMcG0006639 dd_Smed_v4_1071_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30016186h1SMcG0006639 dd_Smed_v4_1071_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016186h1SMcG0006639 dd_Smed_v4_1071_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016186h1SMcG0006639 dd_Smed_v4_1071_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30016186h1SMcG0006639 dd_Smed_v4_1071_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30016186h1SMcG0006639 dd_Smed_v4_1071_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30016186h1SMcG0006639 dd_1071dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult fluorescence in situ hybridization evidence
head regionSMED30016186h1SMcG0006639 dd_Smed_v6_1071_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30016186h1SMcG0006639 OX_Smed_1.0.09131ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
head regionSMED30016186h1SMcG0006639 OX_Smed_1.0.14492ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
pharynxSMED30016186h1SMcG0006639 dd_Smed_v6_1071_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx progenitor cellSMED30016186h1SMcG0006639 dd_Smed_v6_1071_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30016186h1SMcG0006639 dd_Smed_v4_1071_0_1dd_Smed_v4PMID:30962434
Cote et al., 2019
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30016186 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.007402:6526..13466 -5456
v31.007402:16237..20271 -5406
v31.047784:589..1887 +5386
Homology
BLAST of SMED30016186 vs. Planmine SMEST
Match: SMESG000033673.1 (SMESG000033673.1)

HSP 1 Score: 720.309 bits (1858), Expect = 0.000e+0
Identity = 368/371 (99.19%), Postives = 370/371 (99.73%), Query Frame = 2
Query:    2 EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNTKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYK 1114
            EKC+NNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGN TKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVN+KTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYK
Sbjct:  517 EKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNITKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYK 887          

HSP 2 Score: 666.381 bits (1718), Expect = 0.000e+0
Identity = 343/371 (92.45%), Postives = 352/371 (94.88%), Query Frame = 2
Query:    2 EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNTKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYK 1114
            EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRC+KPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKK AIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGN TKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEG KCVLKVVN+KTVNCKN CPLPKE+++RCI+G Q VM++TF  VGN C     RV + KCQ C K SYK
Sbjct:  889 EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCRKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKLAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNITKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGNKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCQKTSYK 1259          

HSP 3 Score: 652.899 bits (1683), Expect = 0.000e+0
Identity = 337/357 (94.40%), Postives = 346/357 (96.92%), Query Frame = 2
Query:    2 EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNTKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARV 1072
            EKC+NNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVN+KTVNCKN CPLPKE+++RCI+G Q VM++TF  VG  C  S  R+
Sbjct: 1599 EKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVG--CPRSVYRI 1953          

HSP 4 Score: 614.379 bits (1583), Expect = 0.000e+0
Identity = 325/371 (87.60%), Postives = 330/371 (88.95%), Query Frame = 2
Query:    2 EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNTKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYK 1114
            EKC+NNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRC+KPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQ   N                                  I+  LS RCTGNTTKNINVVVGKQCQ+GYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVN+KTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARV LVKCQGCPKPSYK
Sbjct: 1261 EKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCRKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQDSFN----------------------------------ILLILSGRCTGNTTKNINVVVGKQCQSGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVRLVKCQGCPKPSYK 1597          

HSP 5 Score: 369.777 bits (948), Expect = 1.939e-115
Identity = 181/371 (48.79%), Postives = 261/371 (70.35%), Query Frame = 2
Query:    2 EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNTKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYK 1114
            EKCD   K T L+R  Y++TN +CV+ +  V KL C+C  N   N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V SK V CK +CP PKEK+SRCI  KQA+ R+TF+ VG+ C +S  R+ +V CQGC   +Y++EKCD   K T L+R  Y++TN +CV+ +  V KL C+C  NT  N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V +K V CK +CP PKEK+SRCI  K A+ R+TF+ VG+ C +S  R+ +V CQGC   +Y+
Sbjct:  703 EKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNITKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYKVEKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCRKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKLAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYR 1073          

HSP 6 Score: 354.755 bits (909), Expect = 3.427e-110
Identity = 174/363 (47.93%), Postives = 255/363 (70.25%), Query Frame = 2
Query:    2 EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNTKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQ 1090
            EKCD   K T L+R  Y++TN +CV+ +  V KL C+C  N   N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E N+C+ K+V SK V CK  CP PKE+R+RCI   Q + ++TF  VG+KC    +R+++  CQ CQ ++Y++EKC+   K T L+R  Y++TN +CV+ +  V KL C+C  NT  N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V +K V CK +CP PKEK+SRCI  KQA+ R+TF+ VG+ C +S  R+ +V CQ
Sbjct: 1075 EKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNITKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGNKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCQKTSYKVEKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCRKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQ 1437          

HSP 7 Score: 342.043 bits (876), Expect = 7.597e-106
Identity = 171/376 (45.48%), Postives = 255/376 (67.82%), Query Frame = 2
Query:   23 KNTSLIRVAYRQTNSSCVRTQTTVTKLPCQ------------CYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNTKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYK 1114
            K  ++ RV +++    C  +   ++ +PCQ            C  NT  N+ V  G+QCQ+G+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V SK V CK +CP PKEK+SRCI  KQA+ R+TF+ VG+ C +S  R+ +V CQGC   +Y++EKC+   K T L+R  Y++TN +CV+ +  V KL C+C  NT  N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V +K V CK +CP PKEK+SRCI  KQA+ R+TF+ VG+ C +S  R+ +V CQGC   +Y+
Sbjct: 1408 KKQAIFRVTFKEVGDKCRFSVIRLSVVPCQDSFNILLILSGRCTGNTTKNINVVVGKQCQSGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVRLVKCQGCPKPSYKVEKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYR 1783          

HSP 8 Score: 300.827 bits (769), Expect = 1.638e-91
Identity = 159/370 (42.97%), Postives = 235/370 (63.51%), Query Frame = 2
Query:    2 EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGK-KCVLKVVNTKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPS 1108
            EKCD   K T L+R  Y++TN +CV+ +  V KL C+C  NT  N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V SK V CK  CP PKE+R+RCI   Q + ++TF  VG                 C  S YRI++C+K AKET L+RTF+Q  N  C++KE+ +K L C+C  N T+NI V++G  CQNGY +RVLEL  E+N  C+K  FKLR I+C+  C+R    +V  C  NKRL++ +K +K     C  K++++  VNC + CP P+ K + CI G++ ++R++++  G  C     ++  +KCQ C + S
Sbjct: 1785 EKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVG-----------------CPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNR-TNQIVGPCFLNKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKS 2136          

HSP 9 Score: 228.024 bits (580), Expect = 2.802e-66
Identity = 138/414 (33.33%), Postives = 215/414 (51.93%), Query Frame = 2
Query:   26 NTSLIRVA-YRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCP------------------------------------------------DPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQ--GCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNTKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYK 1114
            N S I    + + N  C      + K  C C +  E+ + V +G++C+NG+  R++        KCS+    L  ++C   C+    V    C + +R+ T+   V   N+C  KIV +    C  +CP                                                 PK   S CI  K+ + +++++ VG KC+    R+    C    C   +Y++EKC+   K T L+R  Y++TN +CV+ +  V KL C+C  NT  N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V +K V CK +CP PKEK+SRCI  KQA+ R+TF+ VG+ C +S  R+ +V CQGC   +Y+
Sbjct:  289 NYSFILFHNFSRVNDLCRTETVNLKKYSCGCSKPEESVLKVVYGKECENGYKKRMLLYYEFSQFKCSETYLNLEPVKCDTLCNGTTLV-TSPCVNGKRNNTIYTKVFTNNKCSLKIVKTTESFCDTICPKSLIIESKECVNNIKDVFHITFQNVNGKCIKQTKKIKSNNCTKIQECPQPKFISSDCIEGKKILMKLSYRLVGGKCQEINERIGNENCSIAKCPKPSYKVEKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYR 701          

HSP 10 Score: 186.422 bits (472), Expect = 5.880e-52
Identity = 108/324 (33.33%), Postives = 181/324 (55.86%), Query Frame = 2
Query:    2 EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKN-RCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGC-QNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNTKTVNCKNLCP 967
            ++C+ N K T LIR  ++  N  C+R +  +  LPC+CY N   N+TV  G  CQNG+ SRV+EL VEK+  C K  FKLRSI+C+  C+R  ++ VG C  N+R + VL+ ++  N  C WK+++S FV C   CP+P+ K + CI  ++ + R++++E G KC     +L  + CQ C + S Y + +C K +K ++LVRT    +++ C +K   +  + C C G     +NV     C NG   + L      N KC   +  ++  +C    S      ++ CV+  R++++    + G +C+ K+V ++ + C+++CP
Sbjct: 1954 DRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQI-VGPCFLNKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRT----SHRNCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTVNGKCQVKSLSIKGGKCGCLGS---TFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICP 2269          

HSP 11 Score: 85.8853 bits (211), Expect = 7.722e-18
Identity = 76/244 (31.15%), Postives = 105/244 (43.03%), Query Frame = 2
Query:  404 CPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQG---CQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNTKTVNCKNLCP--LPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARV---GLVKCQGCPKPSY 1111
            C  P  K+  C+ K+Q     TF     KC  S  R   V C G   C +  Y+   C        L   F    N  C  + V +KK SC C+      + VV GK+C+NGY  R+L   E    KC +    L  ++C   C+    LV + CV+ KR       V    KC LK+V T    C  +CP  L  E K  C+   + V  +TF+NV   C+  T ++      K Q CP+P +
Sbjct:  229 CSAPIVKKGPCVNKEQINVITTFYYYNGKC-VSENRKLTVKCLGPKDCPDPYYQKTSCGSANYSFILFHNF-SRVNDLCRTETVNLKKYSCGCSKPEESVLKVVYGKECENGYKKRMLLYYEFSQFKCSETYLNLEPVKCDTLCN-GTTLVTSPCVNGKRNNTIYTKVFTNNKCSLKIVKTTESFCDTICPKSLIIESKE-CVNNIKDVFHITFQNVNGKCIKQTKKIKSNNCTKIQECPQPKF 468          
BLAST of SMED30016186 vs. Planmine SMEST
Match: SMESG000033673.1 (SMESG000033673.1)

HSP 1 Score: 719.153 bits (1855), Expect = 0.000e+0
Identity = 368/371 (99.19%), Postives = 370/371 (99.73%), Query Frame = 2
Query:    2 EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNTKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYK 1114
            EKC+NNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGN TKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVN+KTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYK
Sbjct:  517 EKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNITKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYK 887          

HSP 2 Score: 714.531 bits (1843), Expect = 0.000e+0
Identity = 366/371 (98.65%), Postives = 370/371 (99.73%), Query Frame = 2
Query:    2 EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNTKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYK 1114
            EKC+NNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRC+KPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQ+GYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVN+KTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARV LVKCQGCPKPSYK
Sbjct: 1261 EKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCRKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQSGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVRLVKCQGCPKPSYK 1631          

HSP 3 Score: 669.848 bits (1727), Expect = 0.000e+0
Identity = 344/371 (92.72%), Postives = 355/371 (95.69%), Query Frame = 2
Query:    2 EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNTKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYK 1114
            EKC+NNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVN+KTVNCKN CPLPKE+++RCI+G Q VM++TF  VGN C     RV + KCQ CP+  Y+
Sbjct: 1633 EKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYR 2003          

HSP 4 Score: 665.226 bits (1715), Expect = 0.000e+0
Identity = 343/371 (92.45%), Postives = 352/371 (94.88%), Query Frame = 2
Query:    2 EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNTKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYK 1114
            EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRC+KPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKK AIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGN TKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEG KCVLKVVN+KTVNCKN CPLPKE+++RCI+G Q VM++TF  VGN C     RV + KCQ C K SYK
Sbjct:  889 EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCRKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKLAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNITKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGNKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCQKTSYK 1259          

HSP 5 Score: 369.007 bits (946), Expect = 2.944e-115
Identity = 181/371 (48.79%), Postives = 261/371 (70.35%), Query Frame = 2
Query:    2 EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNTKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYK 1114
            EKCD   K T L+R  Y++TN +CV+ +  V KL C+C  N   N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V SK V CK +CP PKEK+SRCI  KQA+ R+TF+ VG+ C +S  R+ +V CQGC   +Y++EKCD   K T L+R  Y++TN +CV+ +  V KL C+C  NT  N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V +K V CK +CP PKEK+SRCI  K A+ R+TF+ VG+ C +S  R+ +V CQGC   +Y+
Sbjct:  703 EKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNITKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYKVEKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCRKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKLAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYR 1073          

HSP 6 Score: 369.007 bits (946), Expect = 3.211e-115
Identity = 181/371 (48.79%), Postives = 263/371 (70.89%), Query Frame = 2
Query:    2 EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNTKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYK 1114
            EKCD   K T L+R  Y++TN +CV+ +  V KL C+C  NT  N+ V  G+QCQ+G+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V SK V CK +CP PKEK+SRCI  KQA+ R+TF+ VG+ C +S  R+ +V CQGC   +Y++EKC+   K T L+R  Y++TN +CV+ +  V KL C+C  NT  N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V +K V CK +CP PKEK+SRCI  KQA+ R+TF+ VG+ C +S  R+ +V CQGC   +Y+
Sbjct: 1447 EKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQSGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVRLVKCQGCPKPSYKVEKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYR 1817          

HSP 7 Score: 362.459 bits (929), Expect = 7.112e-113
Identity = 177/371 (47.71%), Postives = 260/371 (70.08%), Query Frame = 2
Query:    2 EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNTKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYK 1114
            EKCD   K T L+R  Y++TN +CV+ +  V KL C+C  N   N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E N+C+ K+V SK V CK  CP PKE+R+RCI   Q + ++TF  VG+KC    +R+++  CQ CQ ++Y++EKC+   K T L+R  Y++TN +CV+ +  V KL C+C  NT  N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V +K V CK +CP PKEK+SRCI  KQA+ R+TF+ VG+ C +S  R+ +V CQGC   +Y+
Sbjct: 1075 EKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNITKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGNKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCQKTSYKVEKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCRKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYR 1445          

HSP 8 Score: 321.242 bits (822), Expect = 1.179e-98
Identity = 164/370 (44.32%), Postives = 245/370 (66.22%), Query Frame = 2
Query:    2 EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGK-KCVLKVVNTKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPS 1108
            EKCD   K T L+R  Y++TN +CV+ +  V KL C+C  NT  N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V SK V CK  CP PKE+R+RCI   Q + ++TF  VG+KC    +R+++  CQ C  S YRI++C+K AKET L+RTF+Q  N  C++KE+ +K L C+C  N T+NI V++G  CQNGY +RVLEL  E+N  C+K  FKLR I+C+  C+R    +V  C  NKRL++ +K +K     C  K++++  VNC + CP P+ K + CI G++ ++R++++  G  C     ++  +KCQ C + S
Sbjct: 1819 EKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNR-TNQIVGPCFLNKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKS 2187          

HSP 9 Score: 227.639 bits (579), Expect = 4.243e-66
Identity = 138/414 (33.33%), Postives = 215/414 (51.93%), Query Frame = 2
Query:   26 NTSLIRVA-YRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCP------------------------------------------------DPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQ--GCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNTKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYK 1114
            N S I    + + N  C      + K  C C +  E+ + V +G++C+NG+  R++        KCS+    L  ++C   C+    V    C + +R+ T+   V   N+C  KIV +    C  +CP                                                 PK   S CI  K+ + +++++ VG KC+    R+    C    C   +Y++EKC+   K T L+R  Y++TN +CV+ +  V KL C+C  NT  N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V +K V CK +CP PKEK+SRCI  KQA+ R+TF+ VG+ C +S  R+ +V CQGC   +Y+
Sbjct:  289 NYSFILFHNFSRVNDLCRTETVNLKKYSCGCSKPEESVLKVVYGKECENGYKKRMLLYYEFSQFKCSETYLNLEPVKCDTLCNGTTLV-TSPCVNGKRNNTIYTKVFTNNKCSLKIVKTTESFCDTICPKSLIIESKECVNNIKDVFHITFQNVNGKCIKQTKKIKSNNCTKIQECPQPKFISSDCIEGKKILMKLSYRLVGGKCQEINERIGNENCSIAKCPKPSYKVEKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYR 701          

HSP 10 Score: 186.037 bits (471), Expect = 7.927e-52
Identity = 108/324 (33.33%), Postives = 181/324 (55.86%), Query Frame = 2
Query:    2 EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKN-RCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGC-QNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNTKTVNCKNLCP 967
            ++C+ N K T LIR  ++  N  C+R +  +  LPC+CY N   N+TV  G  CQNG+ SRV+EL VEK+  C K  FKLRSI+C+  C+R  ++ VG C  N+R + VL+ ++  N  C WK+++S FV C   CP+P+ K + CI  ++ + R++++E G KC     +L  + CQ C + S Y + +C K +K ++LVRT    +++ C +K   +  + C C G     +NV     C NG   + L      N KC   +  ++  +C    S      ++ CV+  R++++    + G +C+ K+V ++ + C+++CP
Sbjct: 2005 DRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQI-VGPCFLNKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRT----SHRNCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTVNGKCQVKSLSIKGGKCGCLGS---TFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICP 2320          

HSP 11 Score: 85.5001 bits (210), Expect = 9.720e-18
Identity = 76/244 (31.15%), Postives = 105/244 (43.03%), Query Frame = 2
Query:  404 CPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQG---CQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNTKTVNCKNLCP--LPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARV---GLVKCQGCPKPSY 1111
            C  P  K+  C+ K+Q     TF     KC  S  R   V C G   C +  Y+   C        L   F    N  C  + V +KK SC C+      + VV GK+C+NGY  R+L   E    KC +    L  ++C   C+    LV + CV+ KR       V    KC LK+V T    C  +CP  L  E K  C+   + V  +TF+NV   C+  T ++      K Q CP+P +
Sbjct:  229 CSAPIVKKGPCVNKEQINVITTFYYYNGKC-VSENRKLTVKCLGPKDCPDPYYQKTSCGSANYSFILFHNF-SRVNDLCRTETVNLKKYSCGCSKPEESVLKVVYGKECENGYKKRMLLYYEFSQFKCSETYLNLEPVKCDTLCN-GTTLVTSPCVNGKRNNTIYTKVFTNNKCSLKIVKTTESFCDTICPKSLIIESKE-CVNNIKDVFHITFQNVNGKCIKQTKKIKSNNCTKIQECPQPKF 468          
The following BLAST results are available for this feature:
BLAST of SMED30016186 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016186 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016186 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016186 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016186 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016186 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016186 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016186 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016186 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016186 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016186 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016186 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016186 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016186 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000033673.11.939e-11599.19SMESG000033673.1[more]
SMESG000033673.12.944e-11599.19SMESG000033673.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016186 ID=SMED30016186|Name=SMED30016186|organism=Schmidtea mediterranea sexual|type=transcript|length=1116bp
TGAAAAGTGCGATAACAATCAAAAGAACACTTCACTGATTCGTGTTGCGT
ACCGACAGACTAATTCCTCGTGCGTCAGGACGCAGACCACAGTCACAAAA
CTCCCATGCCAATGTTATCGAAATACTGAAACGAATGTAACAGTGACATT
CGGTCGACAATGTCAAAACGGATTCCTGTCGCGAGTCATCGAGTTGAATG
TAGAGAAAAGTGGAAAATGTTCTAAAGTCGCTTTCAAATTGAGAAGTATT
CGATGTAAAAAACCTTGCGATAGACGAAGAAAAGTCGAGGTAGGAAAATG
CAAATCAAATCAAAGAAGTTTGACAGTATTGAGAGCCGTGAGAGAGAAAA
ACCGATGCATGTGGAAAATTGTGACATCGAAATTTGTGAAATGTAAAAAA
GTTTGTCCAGATCCGAAAGAGAAACGATCTCGATGTATTTTGAAAAAGCA
AGCGATTTTCCGAGTGACATTCAAGGAAGTTGGAGACAAATGTAGATTCA
GTGTGATTCGGTTGAGTGTTGTACCTTGTCAAGGTTGTCAAAATAGTACA
TATAGAATCGAGAAGTGTGACAAGGGTGCCAAAGAAACGAAACTCGTTCG
CACATTCTATCAAGAAACAAATCAAACTTGTGTCAAGAAAGAAGTCATTG
TCAAGAAATTATCTTGTCGATGTACCGGAAACACTACAAAAAACATCAAC
GTTGTCGTTGGGAAACAATGTCAAAACGGATACATTACGAGAGTTTTAGA
ATTGACTGAAGAAAGAAACGACAAATGTGTAAAAGTCGCTTTCAAGTTGA
GAAGAATCAGATGTCAAAGACCATGCAGTAGGAAACTGAAGCTCGTCGTC
AATAAATGCGTATCGAATAAGAGATTGATTCAACGCATGAAATCCGTGAA
AGAAGGAAAGAAATGCGTATTGAAGGTCGTCAACACCAAGACAGTGAATT
GTAAGAATCTCTGTCCATTACCGAAAGAAAAGAAATCGAGATGTATTCGA
GGTAAACAGGCAGTGATGAGATTGACATTCAGAAATGTTGGAAATGTTTG
TTTGTATAGTACTGCTCGCGTGGGATTGGTCAAATGTCAAGGATGTCCAA
AGCCTTCATACAAAGT
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protein sequence of SMED30016186-orf-1

>SMED30016186-orf-1 ID=SMED30016186-orf-1|Name=SMED30016186-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=371bp
EKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTF
GRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKC
KSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQ
AIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVR
TFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLE
LTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVK
EGKKCVLKVVNTKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVC
LYSTARVGLVKCQGCPKPSYK
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000110pharynx progenitor cell
PLANA:0000418head
PLANA:0000420parapharyngeal region