60S ribosomal protein L27a

Overview
Name60S ribosomal protein L27a
Smed IDSMED30016163
Length (bp)563
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 60S ribosomal protein L27a (SMED30016163) t-SNE clustered cells

Violin plots show distribution of expression levels for 60S ribosomal protein L27a (SMED30016163) in cells (dots) of each of the 12 neoblast clusters.

 

back to top


 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 60S ribosomal protein L27a (SMED30016163) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 60S ribosomal protein L27a (SMED30016163) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top


 

Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30016163

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 17

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30016163SMESG000034631.1 dd_Smed_v4_155_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30016163SMESG000034631.1 dd_Smed_v4_155_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30016163SMESG000034631.1 dd_Smed_v4_155_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30016163SMESG000034631.1 dd_Smed_v4_155_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016163SMESG000034631.1 dd_Smed_v4_155_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016163SMESG000034631.1 dd_Smed_v4_155_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30016163SMESG000034631.1 dd_Smed_v4_155_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30016163SMESG000034631.1 dd_Smed_v4_155_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30016163SMESG000034631.1 dd_Smed_v4_155_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30016163SMESG000034631.1 dd_Smed_v4_155_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30016163SMESG000034631.1 dd_Smed_v4_155_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016163SMESG000034631.1 Contig36752newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016163SMESG000034631.1 Contig36752uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016163SMESG000034631.1 Contig44028newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016163SMESG000034631.1 Contig44028uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
neoblastSMED30016163SMESG000034631.1 dd_Smed_v6_155_1dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gamma neoblastSMED30016163SMESG000034631.1 dd_Smed_v6_155_1dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of 60S ribosomal protein L27a vs. Ensembl Human
Match: RPL27A (ribosomal protein L27a [Source:HGNC Symbol;Acc:HGNC:10329])

HSP 1 Score: 176.792 bits (447), Expect = 1.248e-56
Identity = 106/145 (73.10%), Postives = 131/145 (90.34%), Query Frame = 2
Query:   86 KVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            +++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+FDK+HPGYFGKVGM+HYHL++N++FCP+VN++K+W+LVS++T      +  G AP+ID +++G +KVLGKG LPK PVIVKAKFFSR+AEEKIKSVGGACVLVA
Sbjct:    4 RLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGMKHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGACVLVA 148          
BLAST of 60S ribosomal protein L27a vs. Ensembl Human
Match: RPL27A (ribosomal protein L27a [Source:HGNC Symbol;Acc:HGNC:10329])

HSP 1 Score: 130.954 bits (328), Expect = 2.300e-39
Identity = 59/91 (64.84%), Postives = 78/91 (85.71%), Query Frame = 2
Query:  248 MRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            M+HYHL++N++FCP+VN++K+W+LVS++T      +  G AP+ID +++G +KVLGKG LPK PVIVKAKFFSR+AEEKIKSVGGACVLVA
Sbjct:    1 MKHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGACVLVA 91          
BLAST of 60S ribosomal protein L27a vs. Ensembl Human
Match: RPL27A (ribosomal protein L27a [Source:HGNC Symbol;Acc:HGNC:10329])

HSP 1 Score: 130.954 bits (328), Expect = 2.300e-39
Identity = 59/91 (64.84%), Postives = 78/91 (85.71%), Query Frame = 2
Query:  248 MRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            M+HYHL++N++FCP+VN++K+W+LVS++T      +  G AP+ID +++G +KVLGKG LPK PVIVKAKFFSR+AEEKIKSVGGACVLVA
Sbjct:    1 MKHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGACVLVA 91          
BLAST of 60S ribosomal protein L27a vs. Ensembl Human
Match: RPL27A (ribosomal protein L27a [Source:HGNC Symbol;Acc:HGNC:10329])

HSP 1 Score: 130.954 bits (328), Expect = 2.300e-39
Identity = 59/91 (64.84%), Postives = 78/91 (85.71%), Query Frame = 2
Query:  248 MRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            M+HYHL++N++FCP+VN++K+W+LVS++T      +  G AP+ID +++G +KVLGKG LPK PVIVKAKFFSR+AEEKIKSVGGACVLVA
Sbjct:    1 MKHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGACVLVA 91          
BLAST of 60S ribosomal protein L27a vs. Ensembl Human
Match: RPL27A (ribosomal protein L27a [Source:HGNC Symbol;Acc:HGNC:10329])

HSP 1 Score: 130.954 bits (328), Expect = 2.300e-39
Identity = 59/91 (64.84%), Postives = 78/91 (85.71%), Query Frame = 2
Query:  248 MRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            M+HYHL++N++FCP+VN++K+W+LVS++T      +  G AP+ID +++G +KVLGKG LPK PVIVKAKFFSR+AEEKIKSVGGACVLVA
Sbjct:    1 MKHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGACVLVA 91          
BLAST of 60S ribosomal protein L27a vs. Ensembl Celegans
Match: Y37E3.8 (pep chromosome:WBcel235:I:2069681:2070607:-1 gene:WBGene00021350.1 transcript:Y37E3.8a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y37E3.8)

HSP 1 Score: 162.155 bits (409), Expect = 2.730e-51
Identity = 102/144 (70.83%), Postives = 122/144 (84.72%), Query Frame = 2
Query:   89 VKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            ++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGGQHHHRI+ DK+HPGYFGKVGMR +HL KN+ +CP+VN+E++WSLV  +  D       GK+PVIDC K G FKVLGKG+LP+ P+IVKA+FFS +AE+KIK  GGACVLVA
Sbjct:    5 LRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGQHHHRINRDKYHPGYFGKVGMRVFHLNKNQHYCPTVNVERLWSLVPQEVRDKATG---GKSPVIDCTKLGYFKVLGKGLLPETPLIVKARFFSHEAEQKIKKAGGACVLVA 145          
BLAST of 60S ribosomal protein L27a vs. Ensembl Celegans
Match: Y37E3.8 (pep chromosome:WBcel235:I:2069683:2070209:-1 gene:WBGene00021350.1 transcript:Y37E3.8b.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y37E3.8)

HSP 1 Score: 115.931 bits (289), Expect = 7.380e-34
Identity = 55/91 (60.44%), Postives = 70/91 (76.92%), Query Frame = 2
Query:  248 MRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            MR +HL KN+ +CP+VN+E++WSLV  +  D       GK+PVIDC K G FKVLGKG+LP+ P+IVKA+FFS +AE+KIK  GGACVLVA
Sbjct:    1 MRVFHLNKNQHYCPTVNVERLWSLVPQEVRDKATG---GKSPVIDCTKLGYFKVLGKGLLPETPLIVKARFFSHEAEQKIKKAGGACVLVA 88          
BLAST of 60S ribosomal protein L27a vs. Ensembl Celegans
Match: Y37E3.8 (pep chromosome:WBcel235:I:2069683:2070574:-1 gene:WBGene00021350.1 transcript:Y37E3.8b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y37E3.8)

HSP 1 Score: 115.931 bits (289), Expect = 7.380e-34
Identity = 55/91 (60.44%), Postives = 70/91 (76.92%), Query Frame = 2
Query:  248 MRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            MR +HL KN+ +CP+VN+E++WSLV  +  D       GK+PVIDC K G FKVLGKG+LP+ P+IVKA+FFS +AE+KIK  GGACVLVA
Sbjct:    1 MRVFHLNKNQHYCPTVNVERLWSLVPQEVRDKATG---GKSPVIDCTKLGYFKVLGKGLLPETPLIVKARFFSHEAEQKIKKAGGACVLVA 88          
BLAST of 60S ribosomal protein L27a vs. Ensembl Fly
Match: RpL27A (gene:FBgn0285948 transcript:FBtr0345285)

HSP 1 Score: 154.066 bits (388), Expect = 5.855e-48
Identity = 98/149 (65.77%), Postives = 120/149 (80.54%), Query Frame = 2
Query:   74 MSKIKVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            MS IK KKTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+FDK+HPGYFGKVGMR++HLR+   F P +N++K+WSLV  + +   +     KAPVID +K G +K+LG+G LP  PVIVKAK+FS++AE+KIK  GG C+L A
Sbjct:    1 MSNIKRKKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRNFHLRRQHKFRPEINLDKLWSLVGAEKFAELEKEKSTKAPVIDLVKFGYYKLLGRGHLPARPVIVKAKYFSKKAEDKIKKAGGVCLLSA 149          
BLAST of 60S ribosomal protein L27a vs. Ensembl Fly
Match: RpL27A (gene:FBgn0285948 transcript:FBtr0077452)

HSP 1 Score: 154.066 bits (388), Expect = 5.855e-48
Identity = 98/149 (65.77%), Postives = 120/149 (80.54%), Query Frame = 2
Query:   74 MSKIKVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            MS IK KKTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+FDK+HPGYFGKVGMR++HLR+   F P +N++K+WSLV  + +   +     KAPVID +K G +K+LG+G LP  PVIVKAK+FS++AE+KIK  GG C+L A
Sbjct:    1 MSNIKRKKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRNFHLRRQHKFRPEINLDKLWSLVGAEKFAELEKEKSTKAPVIDLVKFGYYKLLGRGHLPARPVIVKAKYFSKKAEDKIKKAGGVCLLSA 149          
BLAST of 60S ribosomal protein L27a vs. Ensembl Fly
Match: RpL27A (gene:FBgn0285948 transcript:FBtr0335136)

HSP 1 Score: 154.066 bits (388), Expect = 5.855e-48
Identity = 98/149 (65.77%), Postives = 120/149 (80.54%), Query Frame = 2
Query:   74 MSKIKVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            MS IK KKTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+FDK+HPGYFGKVGMR++HLR+   F P +N++K+WSLV  + +   +     KAPVID +K G +K+LG+G LP  PVIVKAK+FS++AE+KIK  GG C+L A
Sbjct:    1 MSNIKRKKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRNFHLRRQHKFRPEINLDKLWSLVGAEKFAELEKEKSTKAPVIDLVKFGYYKLLGRGHLPARPVIVKAKYFSKKAEDKIKKAGGVCLLSA 149          
BLAST of 60S ribosomal protein L27a vs. Ensembl Xenopus
Match: rpl27a (ribosomal protein L27a [Source:NCBI gene;Acc:100170552])

HSP 1 Score: 184.882 bits (468), Expect = 6.769e-60
Identity = 110/145 (75.86%), Postives = 133/145 (91.72%), Query Frame = 2
Query:   86 KVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            K++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+FDK+HPGYFGKVGMRHYHL+KN++FCP++N++K+W+LVS++T   +  +PEG AP+I+ + AG +KVLGKG LPK PVIVKAKFFSR+AEEKIKSVGGACVLVA
Sbjct:    6 KLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHLKKNQSFCPTINLDKLWTLVSEQTRLNHAKNPEGPAPIINAVHAGYYKVLGKGKLPKQPVIVKAKFFSRKAEEKIKSVGGACVLVA 150          
BLAST of 60S ribosomal protein L27a vs. Ensembl Xenopus
Match: rpl27a (ribosomal protein L27a [Source:NCBI gene;Acc:100170552])

HSP 1 Score: 184.882 bits (468), Expect = 7.479e-60
Identity = 110/145 (75.86%), Postives = 133/145 (91.72%), Query Frame = 2
Query:   86 KVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            K++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+FDK+HPGYFGKVGMRHYHL+KN++FCP++N++K+W+LVS++T   +  +PEG AP+I+ + AG +KVLGKG LPK PVIVKAKFFSR+AEEKIKSVGGACVLVA
Sbjct:    4 KLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHLKKNQSFCPTINLDKLWTLVSEQTRLNHAKNPEGPAPIINAVHAGYYKVLGKGKLPKQPVIVKAKFFSRKAEEKIKSVGGACVLVA 148          
BLAST of 60S ribosomal protein L27a vs. Ensembl Xenopus
Match: rpl27a (ribosomal protein L27a [Source:NCBI gene;Acc:100170552])

HSP 1 Score: 184.882 bits (468), Expect = 7.649e-60
Identity = 110/145 (75.86%), Postives = 133/145 (91.72%), Query Frame = 2
Query:   86 KVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            K++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+FDK+HPGYFGKVGMRHYHL+KN++FCP++N++K+W+LVS++T   +  +PEG AP+I+ + AG +KVLGKG LPK PVIVKAKFFSR+AEEKIKSVGGACVLVA
Sbjct:    3 KLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHLKKNQSFCPTINLDKLWTLVSEQTRLNHAKNPEGPAPIINAVHAGYYKVLGKGKLPKQPVIVKAKFFSRKAEEKIKSVGGACVLVA 147          
BLAST of 60S ribosomal protein L27a vs. Ensembl Mouse
Match: Rpl27a (ribosomal protein L27A [Source:MGI Symbol;Acc:MGI:1347076])

HSP 1 Score: 176.407 bits (446), Expect = 1.163e-56
Identity = 106/145 (73.10%), Postives = 130/145 (89.66%), Query Frame = 2
Query:   86 KVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            +++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+FDK+HPGYFGKVGMRHYHL++N++FCP+VN++K+W+LVS++T      +  G AP+ID +++G +KVLGKG LPK PVIVKAKFFSR+AEEKIK VGGACVLVA
Sbjct:    4 RLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGVAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKGVGGACVLVA 148          
BLAST of 60S ribosomal protein L27a vs. UniProt/SwissProt
Match: sp|P47830|RL27A_XENLA (60S ribosomal protein L27a OS=Xenopus laevis OX=8355 GN=rpl27a PE=2 SV=2)

HSP 1 Score: 179.874 bits (455), Expect = 2.970e-57
Identity = 109/145 (75.17%), Postives = 131/145 (90.34%), Query Frame = 2
Query:   86 KVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            K++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+FDK+HPGYFGKVGMRHYHL+KN++FCP++N++K+W+LVS++T   +  + EG AP+ID + AG +KVLGKG LPK PVIVKAKFFSR+AEEKIKSVGGACVL A
Sbjct:    4 KLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHLKKNQSFCPTINLDKLWTLVSEQTRLNHAKNLEGPAPIIDAVHAGYYKVLGKGKLPKQPVIVKAKFFSRKAEEKIKSVGGACVLGA 148          
BLAST of 60S ribosomal protein L27a vs. UniProt/SwissProt
Match: sp|Q56K03|RL27A_BOVIN (60S ribosomal protein L27a OS=Bos taurus OX=9913 GN=RPL27A PE=2 SV=3)

HSP 1 Score: 177.178 bits (448), Expect = 3.787e-56
Identity = 106/145 (73.10%), Postives = 130/145 (89.66%), Query Frame = 2
Query:   86 KVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            +++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+FDK+HPGYFGKVGMRHYHL++N++FCP+VN++K+W+LVS++T      +  G AP+ID +++G +KVLGKG LPK PVIVKAKFFSR+AEEKIK VGGACVLVA
Sbjct:    4 RLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKGVGGACVLVA 148          
BLAST of 60S ribosomal protein L27a vs. UniProt/SwissProt
Match: sp|Q5REY2|RL27A_PONAB (60S ribosomal protein L27a OS=Pongo abelii OX=9601 GN=RPL27A PE=2 SV=3)

HSP 1 Score: 176.792 bits (447), Expect = 5.992e-56
Identity = 106/145 (73.10%), Postives = 131/145 (90.34%), Query Frame = 2
Query:   86 KVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            +++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+FDK+HPGYFGKVGM+HYHL++N++FCP+VN++K+W+LVS++T      +  G AP+ID +++G +KVLGKG LPK PVIVKAKFFSR+AEEKIKSVGGACVLVA
Sbjct:    4 RLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGMKHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGACVLVA 148          
BLAST of 60S ribosomal protein L27a vs. UniProt/SwissProt
Match: sp|P46776|RL27A_HUMAN (60S ribosomal protein L27a OS=Homo sapiens OX=9606 GN=RPL27A PE=1 SV=2)

HSP 1 Score: 176.792 bits (447), Expect = 5.992e-56
Identity = 106/145 (73.10%), Postives = 131/145 (90.34%), Query Frame = 2
Query:   86 KVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            +++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+FDK+HPGYFGKVGM+HYHL++N++FCP+VN++K+W+LVS++T      +  G AP+ID +++G +KVLGKG LPK PVIVKAKFFSR+AEEKIKSVGGACVLVA
Sbjct:    4 RLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGMKHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGACVLVA 148          
BLAST of 60S ribosomal protein L27a vs. UniProt/SwissProt
Match: sp|P18445|RL27A_RAT (60S ribosomal protein L27a OS=Rattus norvegicus OX=10116 GN=Rpl27a PE=1 SV=3)

HSP 1 Score: 176.792 bits (447), Expect = 6.612e-56
Identity = 106/145 (73.10%), Postives = 130/145 (89.66%), Query Frame = 2
Query:   86 KVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            +++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+FDK+HPGYFGKVGMRHYHL++N++FCP+VN++K+W+LVS++T      +  G AP+ID +++G +KVLGKG LPK PVIVKAKFFSR+AEEKIK VGGACVLVA
Sbjct:    4 RLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKNGVAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKGVGGACVLVA 148          
BLAST of 60S ribosomal protein L27a vs. TrEMBL
Match: D3PHZ9 (60S ribosomal protein L27a OS=Lepeophtheirus salmonis OX=72036 GN=RL27A PE=2 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 5.449e-64
Identity = 120/149 (80.54%), Postives = 138/149 (92.62%), Query Frame = 2
Query:   74 MSKIKVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            MSK+K  KTRKLRGHVSHGHGRVGKHRKHPGGRGNAGGQHHHRI+FDK+HPGYFGK+GMRHYHL+KN ++CP+VN++++WSLVS+KTY  Y+D  EGKAPVIDC+K G FKVLGKGVLPKVPVIVKA+FFSRQAEEKIK VGGAC++ A
Sbjct:    1 MSKLKPTKTRKLRGHVSHGHGRVGKHRKHPGGRGNAGGQHHHRINFDKYHPGYFGKIGMRHYHLKKNVSWCPTVNLDRVWSLVSEKTYQKYRDDKEGKAPVIDCVKHGYFKVLGKGVLPKVPVIVKARFFSRQAEEKIKKVGGACIITA 149          
BLAST of 60S ribosomal protein L27a vs. TrEMBL
Match: A0A4S2LM24 (Ribosomal_L18e/L15P domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_007592 PE=3 SV=1)

HSP 1 Score: 201.83 bits (512), Expect = 6.262e-63
Identity = 123/149 (82.55%), Postives = 135/149 (90.60%), Query Frame = 2
Query:   74 MSKIKVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            MSKIK KKTRKLRGHVSHGHGRVGKHRKHPGGRGNAG +HHHRI+ DK+HPGY GKVGMRHYH +KN  FCP++NIEKIWSLVS  TY+ YKDH +GKAPVIDCLK G FKVLGKGVLPKVPVIVKA+FFSR+AEEKI++VGGACVL A
Sbjct:   32 MSKIKPKKTRKLRGHVSHGHGRVGKHRKHPGGRGNAGLEHHHRINMDKYHPGYIGKVGMRHYHYKKNPYFCPAINIEKIWSLVSQDTYEKYKDHADGKAPVIDCLKKGYFKVLGKGVLPKVPVIVKARFFSRRAEEKIRAVGGACVLTA 180          
BLAST of 60S ribosomal protein L27a vs. TrEMBL
Match: A0A068YJK8 (Large subunit ribosomal protein L27Ae OS=Echinococcus multilocularis OX=6211 GN=EmuJ_001015500 PE=3 SV=1)

HSP 1 Score: 198.749 bits (504), Expect = 3.718e-62
Identity = 114/149 (76.51%), Postives = 137/149 (91.95%), Query Frame = 2
Query:   74 MSKIKVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            MSKIK KKTRKLRGHVSHG GRVGKHRKHPGGRGNAGG+HHHRI+F+K+HPGYFGK+GM HYHLR+N  +CP++N++K+WSLVSD TY+ YK+  +GKAPVIDC++ G FKVLGKG+LPKVPVIVKA+FFSR+AEE+IK+VGGAC+L A
Sbjct:    1 MSKIKAKKTRKLRGHVSHGMGRVGKHRKHPGGRGNAGGEHHHRINFNKYHPGYFGKIGMPHYHLRRNPYYCPTINLDKLWSLVSDATYEKYKNSKDGKAPVIDCVRKGYFKVLGKGILPKVPVIVKARFFSRRAEERIKAVGGACILTA 149          
BLAST of 60S ribosomal protein L27a vs. TrEMBL
Match: A0A068WCE3 (Large subunit ribosomal protein L27Ae OS=Echinococcus granulosus OX=6210 GN=EgrG_001015500 PE=3 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 1.412e-61
Identity = 113/149 (75.84%), Postives = 137/149 (91.95%), Query Frame = 2
Query:   74 MSKIKVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            MSKIK KKTRKLRGHVSHG GRVGKHRKHPGGRGNAGG+HHHRI+F+K+HPGYFGK+GM HYHL++N  +CP++N++K+WSLVSD TY+ YK+  +GKAPVIDC++ G FKVLGKG+LPKVPVIVKA+FFSR+AEE+IK+VGGAC+L A
Sbjct:    1 MSKIKAKKTRKLRGHVSHGMGRVGKHRKHPGGRGNAGGEHHHRINFNKYHPGYFGKIGMPHYHLKRNPYYCPTINLDKLWSLVSDATYEKYKNSKDGKAPVIDCVRKGYFKVLGKGILPKVPVIVKARFFSRRAEERIKAVGGACILTA 149          
BLAST of 60S ribosomal protein L27a vs. TrEMBL
Match: A0A1I8H897 (Ribosomal_L18e/L15P domain-containing protein OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 195.667 bits (496), Expect = 5.724e-61
Identity = 118/150 (78.67%), Postives = 135/150 (90.00%), Query Frame = 2
Query:   74 MSKIKVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKD-HPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            MSK+K  KTRKLRGHVSHGHGR+GKHRKHP GRGNAGG HHHR +FDK+HPGYFGKVGMRH+HLR+N  +CP+VN +K+WSLVS +TYD YKD + EGK PVIDCL+AG FKVLGKG +PKVPVIVKAKFFS++AEEKIKSVGG+CVLVA
Sbjct:    1 MSKLKPTKTRKLRGHVSHGHGRIGKHRKHPSGRGNAGGAHHHRTNFDKYHPGYFGKVGMRHFHLRRNPYYCPAVNTDKLWSLVSQQTYDKYKDGNKEGKVPVIDCLQAGFFKVLGKGNMPKVPVIVKAKFFSKKAEEKIKSVGGSCVLVA 150          
BLAST of 60S ribosomal protein L27a vs. Ensembl Cavefish
Match: RPL27A (ribosomal protein L27a [Source:HGNC Symbol;Acc:HGNC:10329])

HSP 1 Score: 167.162 bits (422), Expect = 1.182e-53
Identity = 77/111 (69.37%), Postives = 94/111 (84.68%), Query Frame = 2
Query:  188 QHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
             HHHRI+FDK+HPGYFGKVGMRHYHL++N  FCP++N++K+W+LVS++T   +    +G APVID ++AG FKVLGKG LPK PVIVKAKFFSR+AEEKIK VGGACVL A
Sbjct:    1 MHHHRINFDKYHPGYFGKVGMRHYHLKRNTVFCPTINLDKLWTLVSEQTRVNHAKKLDGPAPVIDVVRAGYFKVLGKGKLPKQPVIVKAKFFSRRAEEKIKGVGGACVLTA 111          
BLAST of 60S ribosomal protein L27a vs. Ensembl Yeast
Match: RPL28 (Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance [Source:SGD;Acc:S000003071])

HSP 1 Score: 132.88 bits (333), Expect = 1.810e-40
Identity = 63/115 (54.78%), Postives = 85/115 (73.91%), Query Frame = 2
Query:  179 AGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTY-KDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            AGGQHHHRI+ DK+HPGYFGKVGMR++H ++   + P +N++K+W+L+ +   D Y K   +  APVID L AG  K+LGKG +P VPVIVKA+F S+ AEEKI++ GG   L+A
Sbjct:   35 AGGQHHHRINMDKYHPGYFGKVGMRYFHKQQAHFWKPVLNLDKLWTLIPEDKRDQYLKSASKETAPVIDTLAAGYGKILGKGRIPNVPVIVKARFVSKLAEEKIRAAGGVVELIA 149          
BLAST of 60S ribosomal protein L27a vs. Ensembl Yeast
Match: YGL102C_mRNA (Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein [Source:SGD;Acc:S000003070])

HSP 1 Score: 59.6918 bits (143), Expect = 2.293e-12
Identity = 39/104 (37.50%), Postives = 57/104 (54.81%), Query Frame = -2
Query:  128 NFRQNSFTQNFENTSFQTINNWCFSFRMIFVSIVSFITY*-TPNFLDIYTWAKGTIFS*MIMSHTNFTEITRMEFIKMNSMVMLTTSITTTSRMLPVFAYSTMT 436
            N   ++ TQ+   T  Q++NNW    R  F  +VS + +   P F+ +  W        + +SHTN TEIT M FI +NS+VMLTT  T+T+ +L V   +T T
Sbjct:   32 NIWNSTLTQDLTVTGCQSVNNWSSFLRSRFQVLVSLVFWDQCPQFVQVQDWLPEMSLLLVEVSHTNLTEITWMVFIHVNSVVMLTTGHTSTTGVLSVLTDTTFT 135          
BLAST of 60S ribosomal protein L27a vs. Ensembl Nematostella
Match: EDO47596 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RLF6])

HSP 1 Score: 174.866 bits (442), Expect = 9.751e-57
Identity = 81/115 (70.43%), Postives = 100/115 (86.96%), Query Frame = 2
Query:  176 NAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            NAGGQHHHRI+FDK+HPGYFGKVGMRH+HL +N    PS+N++K+WSLVS++T   YK+  +G  PVID +KAG +KVLGKG+LPK PVIVKAKFFSR+AE+KIK+VGGACVL+A
Sbjct:   20 NAGGQHHHRINFDKYHPGYFGKVGMRHFHLTRNAYHKPSINLDKVWSLVSEQTRQNYKNKKDGPVPVIDVVKAGYYKVLGKGLLPKQPVIVKAKFFSRRAEDKIKAVGGACVLMA 134          
BLAST of 60S ribosomal protein L27a vs. Ensembl Medaka
Match: RPL27A (ribosomal protein L27a [Source:NCBI gene;Acc:101160900])

HSP 1 Score: 184.111 bits (466), Expect = 8.801e-60
Identity = 83/115 (72.17%), Postives = 102/115 (88.70%), Query Frame = 2
Query:  176 NAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            NAGG HHHRI+FDK+HPGYFGKVGMRHYHL++N T+CP++N++K+W+LVS++T   Y   P+G APVID ++AG +KVLGKG LPK PVIVKAKFFSRQAEEKIK+VGGACVL+A
Sbjct:   34 NAGGLHHHRINFDKYHPGYFGKVGMRHYHLKRNTTYCPTINLDKLWTLVSEQTRVNYGKKPDGPAPVIDAVRAGYYKVLGKGKLPKQPVIVKAKFFSRQAEEKIKAVGGACVLMA 148          
BLAST of 60S ribosomal protein L27a vs. Ensembl Medaka
Match: RPL27A (ribosomal protein L27a [Source:NCBI gene;Acc:101160900])

HSP 1 Score: 157.147 bits (396), Expect = 1.418e-49
Identity = 71/100 (71.00%), Postives = 88/100 (88.00%), Query Frame = 2
Query:  221 HPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            HPGYFGKVGMRHYHL++N T+CP++N++K+W+LVS++T   Y   P+G APVID ++AG +KVLGKG LPK PVIVKAKFFSRQAEEKIK+VGGACVL+A
Sbjct:   25 HPGYFGKVGMRHYHLKRNTTYCPTINLDKLWTLVSEQTRVNYGKKPDGPAPVIDAVRAGYYKVLGKGKLPKQPVIVKAKFFSRQAEEKIKAVGGACVLMA 124          
BLAST of 60S ribosomal protein L27a vs. Planmine SMEST
Match: SMESG000034631.1 (SMESG000034631.1)

HSP 1 Score: 249.21 bits (635), Expect = 1.113e-85
Identity = 149/149 (100.00%), Postives = 149/149 (100.00%), Query Frame = 2
Query:   74 MSKIKVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            MSKIKVKKTRKLRGHVSHGHGRVGKHRKHPGGRGNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA
Sbjct:    1 MSKIKVKKTRKLRGHVSHGHGRVGKHRKHPGGRGNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 149          
BLAST of 60S ribosomal protein L27a vs. Planmine SMEST
Match: SMESG000005086.1 (SMESG000005086.1)

HSP 1 Score: 231.491 bits (589), Expect = 1.110e-78
Identity = 139/149 (93.29%), Postives = 145/149 (97.32%), Query Frame = 2
Query:   74 MSKIKVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 520
            MSKIKVKKTRKLRGHVSHGHGRVGKHRKHPGGRGNAGGQHHHRIH DKFHPGYFGKVGMRHYHLRKNRTFCPS+NIEK+WSLVSDKTY+TYK++ EGKAPVID LKAG FK+LGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA
Sbjct:    1 MSKIKVKKTRKLRGHVSHGHGRVGKHRKHPGGRGNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 149          
The following BLAST results are available for this feature:
BLAST of 60S ribosomal protein L27a vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RPL27A1.248e-5673.10ribosomal protein L27a [Source:HGNC Symbol;Acc:HGN... [more]
RPL27A2.300e-3964.84ribosomal protein L27a [Source:HGNC Symbol;Acc:HGN... [more]
RPL27A2.300e-3964.84ribosomal protein L27a [Source:HGNC Symbol;Acc:HGN... [more]
RPL27A2.300e-3964.84ribosomal protein L27a [Source:HGNC Symbol;Acc:HGN... [more]
RPL27A2.300e-3964.84ribosomal protein L27a [Source:HGNC Symbol;Acc:HGN... [more]
back to top
BLAST of 60S ribosomal protein L27a vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Y37E3.82.730e-5170.83pep chromosome:WBcel235:I:2069681:2070607:-1 gene:... [more]
Y37E3.87.380e-3460.44pep chromosome:WBcel235:I:2069683:2070209:-1 gene:... [more]
Y37E3.87.380e-3460.44pep chromosome:WBcel235:I:2069683:2070574:-1 gene:... [more]
back to top
BLAST of 60S ribosomal protein L27a vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
RpL27A5.855e-4865.77gene:FBgn0285948 transcript:FBtr0345285[more]
RpL27A5.855e-4865.77gene:FBgn0285948 transcript:FBtr0077452[more]
RpL27A5.855e-4865.77gene:FBgn0285948 transcript:FBtr0335136[more]
back to top
BLAST of 60S ribosomal protein L27a vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of 60S ribosomal protein L27a vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
rpl27a6.769e-6075.86ribosomal protein L27a [Source:NCBI gene;Acc:10017... [more]
rpl27a7.479e-6075.86ribosomal protein L27a [Source:NCBI gene;Acc:10017... [more]
rpl27a7.649e-6075.86ribosomal protein L27a [Source:NCBI gene;Acc:10017... [more]
back to top
BLAST of 60S ribosomal protein L27a vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Rpl27a1.163e-5673.10ribosomal protein L27A [Source:MGI Symbol;Acc:MGI:... [more]
back to top
BLAST of 60S ribosomal protein L27a vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P47830|RL27A_XENLA2.970e-5775.1760S ribosomal protein L27a OS=Xenopus laevis OX=83... [more]
sp|Q56K03|RL27A_BOVIN3.787e-5673.1060S ribosomal protein L27a OS=Bos taurus OX=9913 G... [more]
sp|Q5REY2|RL27A_PONAB5.992e-5673.1060S ribosomal protein L27a OS=Pongo abelii OX=9601... [more]
sp|P46776|RL27A_HUMAN5.992e-5673.1060S ribosomal protein L27a OS=Homo sapiens OX=9606... [more]
sp|P18445|RL27A_RAT6.612e-5673.1060S ribosomal protein L27a OS=Rattus norvegicus OX... [more]
back to top
BLAST of 60S ribosomal protein L27a vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
D3PHZ95.449e-6480.5460S ribosomal protein L27a OS=Lepeophtheirus salmo... [more]
A0A4S2LM246.262e-6382.55Ribosomal_L18e/L15P domain-containing protein OS=O... [more]
A0A068YJK83.718e-6276.51Large subunit ribosomal protein L27Ae OS=Echinococ... [more]
A0A068WCE31.412e-6175.84Large subunit ribosomal protein L27Ae OS=Echinococ... [more]
A0A1I8H8975.724e-6178.67Ribosomal_L18e/L15P domain-containing protein OS=M... [more]
back to top
BLAST of 60S ribosomal protein L27a vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
RPL27A1.182e-5369.37ribosomal protein L27a [Source:HGNC Symbol;Acc:HGN... [more]
back to top
BLAST of 60S ribosomal protein L27a vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of 60S ribosomal protein L27a vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
RPL281.810e-4054.78Ribosomal 60S subunit protein L28; homologous to m... [more]
YGL102C_mRNA2.293e-1237.50Dubious open reading frame unlikely to encode a fu... [more]
back to top
BLAST of 60S ribosomal protein L27a vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO475969.751e-5770.43Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of 60S ribosomal protein L27a vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
RPL27A8.801e-6072.17ribosomal protein L27a [Source:NCBI gene;Acc:10116... [more]
RPL27A1.418e-4971.00ribosomal protein L27a [Source:NCBI gene;Acc:10116... [more]
back to top
BLAST of 60S ribosomal protein L27a vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000034631.11.113e-85100.00SMESG000034631.1[more]
SMESG000005086.11.110e-7893.29SMESG000005086.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016163 ID=SMED30016163|Name=60S ribosomal protein L27a|organism=Schmidtea mediterranea sexual|type=transcript|length=563bp
TTTAAATTACTTAATTTTTTTATTAAAATTTGTTTTAAATGAGTTTTTGA
AATTGTTTTTCATATTTTGAATTATGTCCAAAATTAAGGTAAAGAAGACC
AGGAAGCTAAGAGGTCATGTTAGCCATGGTCATGGTAGAGTAGGCAAACA
CAGGAAGCATCCTGGAGGTCGTGGTAATGCTGGTGGTCAACATCACCATA
GAATTCATTTTGATAAATTCCATCCTGGTTATTTCGGTAAAGTTGGTATG
AGACATTATCATTTAAGAAAAAATCGTACCTTTTGCCCAAGTGTAAATAT
CGAGAAAATTTGGAGTTTAGTAAGTGATAAAACTTACGATACTTACAAAG
ATCATCCTGAAGGAAAAGCACCAGTTATTGATTGTTTGAAAGCTGGTATT
TTCAAAGTTCTGGGTAAAGGAGTTTTGCCTAAAGTTCCTGTAATAGTTAA
GGCTAAATTCTTCTCGAGACAAGCTGAAGAAAAAATTAAATCAGTTGGTG
GAGCTTGTGTTTTGGTTGCCTAAATAAAAATTAATTTGGATTAAAAAAAA
AAGATCGGAAGAG
back to top

protein sequence of SMED30016163-orf-1

>SMED30016163-orf-1 ID=SMED30016163-orf-1|Name=SMED30016163-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=150bp
MSKIKVKKTRKLRGHVSHGHGRVGKHRKHPGGRGNAGGQHHHRIHFDKFH
PGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAP
VIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000039gamma neoblast
PLANA:0000429neoblast
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR036227L18e/L15P_sf
IPR021131Ribosomal_L18e/L15P
IPR001196Ribosomal_L15_CS
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
Vocabulary: cellular component
TermDefinition
GO:0005622intracellular
GO:0005840ribosome
GO:0015934large ribosomal subunit
GO:0030529intracellular ribonucleoprotein complex
Vocabulary: biological process
TermDefinition
GO:0006412translation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.100.10.10coord: 59..149
e-value: 7.9E-39
score: 133.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..39
NoneNo IPR availablePANTHERPTHR1172160S RIBOSOMAL PROTEIN L27Acoord: 5..149
NoneNo IPR availablePANTHERPTHR11721:SF1360S RIBOSOMAL PROTEIN L27Acoord: 5..149
IPR021131Ribosomal protein L18e/L15PPFAMPF00828Ribosomal_L27Acoord: 29..146
e-value: 3.7E-23
score: 82.6
IPR001196Ribosomal protein L15, conserved sitePROSITEPS00475RIBOSOMAL_L15coord: 111..142
IPR036227Ribosomal L18e/L15P superfamilySUPERFAMILYSSF52080Ribosomal proteins L15p and L18ecoord: 7..148