Small integral membrane protein 14

Overview
NameSmall integral membrane protein 14
Smed IDSMED30016109
Length (bp)538
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Small integral membrane protein 14 (SMED30016109) t-SNE clustered cells

Violin plots show distribution of expression levels for Small integral membrane protein 14 (SMED30016109) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Small integral membrane protein 14 (SMED30016109) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Small integral membrane protein 14 (SMED30016109) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30016109

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30016109SMESG000040023.1 dd_Smed_v4_2632_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30016109SMESG000040023.1 dd_Smed_v4_2632_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30016109SMESG000040023.1 dd_Smed_v4_2632_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Small integral membrane protein 14 vs. Ensembl Human
Match: SMIM14 (small integral membrane protein 14 [Source:HGNC Symbol;Acc:HGNC:27321])

HSP 1 Score: 57.7658 bits (138), Expect = 5.912e-11
Identity = 44/102 (43.14%), Postives = 57/102 (55.88%), Query Frame = 1 
Query:   88 QSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDTSSDPMNNFXXXXXXXXXXXAMVGMLLAGYRP--GRRNRLPEKPSRIDQNRNDEDPP 387
            + GFD CEC+  HE+AMRRL+NLLRQSQSYCTD  C Q LPGP   +   +          L    ++ ++L   RP   R + LP KP+      N +DPP
Sbjct:    3 EGGFDPCECVCSHEHAMRRLINLLRQSQSYCTDTECLQELPGPSGDNGISVTMI-------LVAWMVIALILFLLRPPNLRGSSLPGKPT---SPHNGQDPP 94
[Close]
BLAST of Small integral membrane protein 14 vs. Ensembl Human
Match: SMIM14 (small integral membrane protein 14 [Source:HGNC Symbol;Acc:HGNC:27321])

HSP 1 Score: 55.4546 bits (132), Expect = 4.240e-10
Identity = 40/90 (44.44%), Postives = 52/90 (57.78%), Query Frame = 1 
Query:   88 QSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDTSSDPMNNFXXXXXXXXXXXAMVGMLLAGYRPG--RRNRLPEKPS 351
            + GFD CEC+  HE+AMRRL+NLLRQSQSYCTD  C Q LPGP   +   +          L    ++ ++L   RP   R + LP KP+
Sbjct:    3 EGGFDPCECVCSHEHAMRRLINLLRQSQSYCTDTECLQELPGPSGDNGISVTMI-------LVAWMVIALILFLLRPPNLRGSSLPGKPT 85
[Close]
BLAST of Small integral membrane protein 14 vs. Ensembl Human
Match: SMIM14 (small integral membrane protein 14 [Source:HGNC Symbol;Acc:HGNC:27321])

HSP 1 Score: 44.669 bits (104), Expect = 1.754e-6
Identity = 27/38 (71.05%), Postives = 31/38 (81.58%), Query Frame = 1 
Query:   88 QSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQ 201
            + GFD CEC+  HE+AMRRL+NLLRQSQSYCTD  C Q
Sbjct:    3 EGGFDPCECVCSHEHAMRRLINLLRQSQSYCTDTECLQ 40
[Close]
BLAST of Small integral membrane protein 14 vs. Ensembl Zebrafish
Match: smim14 (small integral membrane protein 14 [Source:ZFIN;Acc:ZDB-GENE-040426-22])

HSP 1 Score: 53.1434 bits (126), Expect = 2.273e-9
Identity = 31/46 (67.39%), Postives = 36/46 (78.26%), Query Frame = 1 
Query:   88 QSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDT 225
            + GFD CECI  HE+AMRRL+NLLRQSQSYCTD  C Q +PGP  +
Sbjct:    3 EGGFDPCECICSHEHAMRRLINLLRQSQSYCTDTECLQDMPGPNSS 48
[Close]
BLAST of Small integral membrane protein 14 vs. Ensembl Zebrafish
Match: smim14 (small integral membrane protein 14 [Source:ZFIN;Acc:ZDB-GENE-040426-22])

HSP 1 Score: 53.1434 bits (126), Expect = 2.273e-9
Identity = 31/46 (67.39%), Postives = 36/46 (78.26%), Query Frame = 1 
Query:   88 QSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDT 225
            + GFD CECI  HE+AMRRL+NLLRQSQSYCTD  C Q +PGP  +
Sbjct:    3 EGGFDPCECICSHEHAMRRLINLLRQSQSYCTDTECLQDMPGPNSS 48
[Close]
BLAST of Small integral membrane protein 14 vs. Ensembl Zebrafish
Match: smim14 (small integral membrane protein 14 [Source:ZFIN;Acc:ZDB-GENE-040426-22])

HSP 1 Score: 54.299 bits (129), Expect = 2.353e-9
Identity = 31/46 (67.39%), Postives = 36/46 (78.26%), Query Frame = 1 
Query:   88 QSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDT 225
            + GFD CECI  HE+AMRRL+NLLRQSQSYCTD  C Q +PGP  +
Sbjct:   42 EGGFDPCECICSHEHAMRRLINLLRQSQSYCTDTECLQDMPGPNSS 87
[Close]
BLAST of Small integral membrane protein 14 vs. Ensembl Xenopus
Match: prim2 (DNA primase subunit 2 [Source:Xenbase;Acc:XB-GENE-1004020])

HSP 1 Score: 56.9954 bits (136), Expect = 1.214e-10
Identity = 46/103 (44.66%), Postives = 57/103 (55.34%), Query Frame = 1 
Query:   88 QSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDTSSDPMNNFXXXXXXXXXXXAMVGMLLAGYRPG--RRNRLPEKPSRIDQNRNDEDPPP 390
            +  FD CECI  HE+AMRRL+NLLRQSQSYCTDN C Q LPGP  ++    N     I +      ++G+ L   RP   R N    KP+    N+  E P P
Sbjct:    3 EGNFDPCECICSHEHAMRRLINLLRQSQSYCTDNECFQELPGPNSSTDGGFN-----IAMIAMAWLVIGVTLYLLRPRSLRGNGPSAKPNSPHSNQGPEPPAP 100
[Close]
BLAST of Small integral membrane protein 14 vs. Ensembl Mouse
Match: Smim14 (small integral membrane protein 14 [Source:MGI Symbol;Acc:MGI:1915802])

HSP 1 Score: 60.077 bits (144), Expect = 6.632e-12
Identity = 45/102 (44.12%), Postives = 56/102 (54.90%), Query Frame = 1 
Query:   88 QSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDTSSDPMNNFXXXXXXXXXXXAMVGMLLAGYRP--GRRNRLPEKPSRIDQNRNDEDPP 387
            + GFD CEC+  HE+AMRRL+NLLRQSQSYCTD  C + LPGP   S   +          L    ++ MLL   RP   R + LP KPS     ++   PP
Sbjct:    3 EGGFDPCECVCSHEHAMRRLINLLRQSQSYCTDTECLRELPGPSSDSGISITVI-------LMAWMVIAMLLFLLRPPNLRGSSLPGKPSSPHSGQDPPAPP 97
[Close]
BLAST of Small integral membrane protein 14 vs. Ensembl Mouse
Match: Smim14 (small integral membrane protein 14 [Source:MGI Symbol;Acc:MGI:1915802])

HSP 1 Score: 60.077 bits (144), Expect = 6.632e-12
Identity = 45/102 (44.12%), Postives = 56/102 (54.90%), Query Frame = 1 
Query:   88 QSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDTSSDPMNNFXXXXXXXXXXXAMVGMLLAGYRP--GRRNRLPEKPSRIDQNRNDEDPP 387
            + GFD CEC+  HE+AMRRL+NLLRQSQSYCTD  C + LPGP   S   +          L    ++ MLL   RP   R + LP KPS     ++   PP
Sbjct:    3 EGGFDPCECVCSHEHAMRRLINLLRQSQSYCTDTECLRELPGPSSDSGISITVI-------LMAWMVIAMLLFLLRPPNLRGSSLPGKPSSPHSGQDPPAPP 97
[Close]
BLAST of Small integral membrane protein 14 vs. Ensembl Mouse
Match: Smim14 (small integral membrane protein 14 [Source:MGI Symbol;Acc:MGI:1915802])

HSP 1 Score: 58.9214 bits (141), Expect = 1.249e-11
Identity = 43/90 (47.78%), Postives = 52/90 (57.78%), Query Frame = 1 
Query:   88 QSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDTSSDPMNNFXXXXXXXXXXXAMVGMLLAGYRP--GRRNRLPEKPS 351
            + GFD CEC+  HE+AMRRL+NLLRQSQSYCTD  C + LPGP   S   +          L    ++ MLL   RP   R + LP KPS
Sbjct:    3 EGGFDPCECVCSHEHAMRRLINLLRQSQSYCTDTECLRELPGPSSDSGISITVI-------LMAWMVIAMLLFLLRPPNLRGSSLPGKPS 85
[Close]
BLAST of Small integral membrane protein 14 vs. UniProt/SwissProt
Match: sp|Q91VT8|SIM14_MOUSE (Small integral membrane protein 14 OS=Mus musculus OX=10090 GN=Smim14 PE=1 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 4.639e-11
Identity = 45/102 (44.12%), Postives = 56/102 (54.90%), Query Frame = 1 
Query:   88 QSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDTSSDPMNNFXXXXXXXXXXXAMVGMLLAGYRP--GRRNRLPEKPSRIDQNRNDEDPP 387
            + GFD CEC+  HE+AMRRL+NLLRQSQSYCTD  C + LPGP   S   +          L    ++ MLL   RP   R + LP KPS     ++   PP
Sbjct:    3 EGGFDPCECVCSHEHAMRRLINLLRQSQSYCTDTECLRELPGPSSDSGISITVI-------LMAWMVIAMLLFLLRPPNLRGSSLPGKPSSPHSGQDPPAPP 97
[Close]
BLAST of Small integral membrane protein 14 vs. UniProt/SwissProt
Match: sp|Q498C7|SIM14_RAT (Small integral membrane protein 14 OS=Rattus norvegicus OX=10116 GN=Smim14 PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 1.388e-10
Identity = 45/102 (44.12%), Postives = 56/102 (54.90%), Query Frame = 1 
Query:   88 QSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDTSSDPMNNFXXXXXXXXXXXAMVGMLLAGYRP--GRRNRLPEKPSRIDQNRNDEDPP 387
            + GFD CECI  HE+AMRRL+NLLRQSQSYCTD  C + LPGP   S   +          L    ++ +LL   RP   R + LP KPS     ++   PP
Sbjct:    3 EGGFDPCECICSHEHAMRRLINLLRQSQSYCTDTECLRELPGPSGDSGISITVI-------LMAWMVIAVLLFLLRPPNLRGSSLPGKPSSPHSGQDPPAPP 97
[Close]
BLAST of Small integral membrane protein 14 vs. UniProt/SwissProt
Match: sp|Q96QK8|SIM14_HUMAN (Small integral membrane protein 14 OS=Homo sapiens OX=9606 GN=SMIM14 PE=1 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 2.839e-10
Identity = 44/102 (43.14%), Postives = 57/102 (55.88%), Query Frame = 1 
Query:   88 QSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDTSSDPMNNFXXXXXXXXXXXAMVGMLLAGYRP--GRRNRLPEKPSRIDQNRNDEDPP 387
            + GFD CEC+  HE+AMRRL+NLLRQSQSYCTD  C Q LPGP   +   +          L    ++ ++L   RP   R + LP KP+      N +DPP
Sbjct:    3 EGGFDPCECVCSHEHAMRRLINLLRQSQSYCTDTECLQELPGPSGDNGISVTMI-------LVAWMVIALILFLLRPPNLRGSSLPGKPT---SPHNGQDPP 94
[Close]
BLAST of Small integral membrane protein 14 vs. UniProt/SwissProt
Match: sp|Q5RF07|SIM14_PONAB (Small integral membrane protein 14 OS=Pongo abelii OX=9601 GN=SMIM14 PE=3 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 3.289e-10
Identity = 44/102 (43.14%), Postives = 57/102 (55.88%), Query Frame = 1 
Query:   88 QSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDTSSDPMNNFXXXXXXXXXXXAMVGMLLAGYRP--GRRNRLPEKPSRIDQNRNDEDPP 387
            + GFD CEC+  HE+AMRRL+NLLRQSQSYCTD  C Q LPGP   +   +          L    ++ ++L   RP   R + LP KP+      N +DPP
Sbjct:    3 EGGFDPCECVCSHEHAMRRLINLLRQSQSYCTDTECLQELPGPSGDNGISVTMI-------LVAWMVIALILFLLRPPNLRGSNLPGKPT---SPHNGQDPP 94
[Close]
BLAST of Small integral membrane protein 14 vs. UniProt/SwissProt
Match: sp|Q2KIK3|SIM14_BOVIN (Small integral membrane protein 14 OS=Bos taurus OX=9913 GN=SMIM14 PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 4.433e-9
Identity = 43/102 (42.16%), Postives = 56/102 (54.90%), Query Frame = 1 
Query:   88 QSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDTSSDPMNNFXXXXXXXXXXXAMVGMLLAGYRP--GRRNRLPEKPSRIDQNRNDEDPP 387
            + GFD CEC+  HE+AMRRL+NLLRQSQSYCTD  C Q LPGP   +   +          L    ++ ++L   RP   R + L  KP+      N +DPP
Sbjct:    3 EGGFDPCECVCSHEHAMRRLINLLRQSQSYCTDTECLQELPGPSSDNGISITMI-------LMAWMVIAVILFLLRPPNLRGSNLTGKPA---SPHNGQDPP 94
[Close]
BLAST of Small integral membrane protein 14 vs. TrEMBL
Match: A0A2B4RQ32 (Small integral membrane protein 14 OS=Stylophora pistillata OX=50429 GN=SMIM14 PE=4 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.323e-10
Identity = 48/99 (48.48%), Postives = 56/99 (56.57%), Query Frame = 1 
Query:   79 MDDQSG--FDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQ--DTSSDPMNNFXXXXXXXXXXXAMVGMLLAGYRPGRRNRLPEKPSRIDQ 363
            MD   G  FD CECI  HE AMRRL++LLR SQSYCTDN C Q LPGPQ  DT  D    F       +    ++  +L   RP    + PEKPSR D+
Sbjct:    1 MDGGGGDSFDPCECICSHEGAMRRLISLLRSSQSYCTDNQCLQELPGPQGNDTGGDSHTAF-----YIMIAWLIIAFVLYLLRPQSMRQGPEKPSRSDR 94
[Close]
BLAST of Small integral membrane protein 14 vs. TrEMBL
Match: A0A1Y1KUW6 (Uncharacterized protein OS=Photinus pyralis OX=7054 GN=PPYR_11792 PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.976e-10
Identity = 48/105 (45.71%), Postives = 61/105 (58.10%), Query Frame = 1 
Query:   79 MDDQSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDTSSDPMNNFXXXXXXXXXXXAMVGMLLAGYRPGRRN-RLPEKPSRIDQNRNDEDPPP 390
            M D+ GFD CEC++GHE AMRRLL+LLRQSQS+CTDN C QT  G Q+ S+D   NF    ++         +L+  +RP   +   P KPS  D   N   P P
Sbjct:    1 MADEGGFDPCECVWGHEMAMRRLLSLLRQSQSHCTDNECFQTADGNQNLSTD---NFVFTTLIIA-----FALLMYYFRPQSASLEGPSKPSNNDNGSNGFPPTP 97
[Close]
BLAST of Small integral membrane protein 14 vs. TrEMBL
Match: E0VG88 (Uncharacterized protein OS=Pediculus humanus subsp. corporis OX=121224 GN=8236787 PE=4 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.145e-9
Identity = 46/107 (42.99%), Postives = 56/107 (52.34%), Query Frame = 1 
Query:   85 DQSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDTSSDPMNNFXXXXXXXXXXXAMVGMLLAGYRPGRRNRLPEKPSRIDQNRND-----EDPPP 390
            D +GFD CECI+ H  AMRRLL++LRQSQ+YCTDN C   LPG    S  P  NF            ++ MLL   RP    R  +   ++  N ND      DPPP
Sbjct:    2 DGNGFDPCECIWNHNMAMRRLLSILRQSQAYCTDNECLNELPGSNIDS--PEQNF----FFVAAFWFLIAMLLYFLRPNTFRRREDDILKLRNNENDSSDNSSDPPP 102
[Close]
BLAST of Small integral membrane protein 14 vs. TrEMBL
Match: A0A1W7RIU7 (Putative C4orf34-like protein OS=Agkistrodon contortrix contortrix OX=8713 PE=4 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.412e-9
Identity = 46/100 (46.00%), Postives = 63/100 (63.00%), Query Frame = 1 
Query:   88 QSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDTSSDPMNNFXXXXXXXXXXXAMVGMLLAGYRPGRRNRLPEKPSRIDQNRNDEDPP 387
            + GFD CECI  HE+AMRRL++LLRQSQSYCTD  C Q LPGP  ++    N+F + +++  WL+  + + L      RR+   EKPSR     + +DPP
Sbjct:    3 EGGFDPCECICAHEHAMRRLIDLLRQSQSYCTDTECLQELPGPNPSAD---NDFSVTVMMVGWLVLALVLFLMRPTSLRRSITTEKPSR---PHSGQDPP 96
[Close]
BLAST of Small integral membrane protein 14 vs. TrEMBL
Match: T1DM56 (Putative C4orf34-like protein OS=Crotalus horridus OX=35024 PE=2 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 2.422e-9
Identity = 47/100 (47.00%), Postives = 64/100 (64.00%), Query Frame = 1 
Query:   88 QSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDTSSDPMNNFXXXXXXXXXXXAMVGMLLAGYRPGRRNRLPEKPSRIDQNRNDEDPP 387
            + GFD CECI  HE+AMRRL++LLRQSQSYCTD  C Q LPGP  ++    N+FG+ +++  WL+  + + L      RR+   EKPSR     + +DPP
Sbjct:    3 EGGFDPCECICAHEHAMRRLIDLLRQSQSYCTDTECLQELPGPNPSAD---NDFGVTMMMVGWLVLALVLFLMRPTSLRRSITAEKPSR---PHSGQDPP 96
[Close]
BLAST of Small integral membrane protein 14 vs. Ensembl Cavefish
Match: smim14 (small integral membrane protein 14 [Source:NCBI gene;Acc:103045654])

HSP 1 Score: 58.5362 bits (140), Expect = 1.767e-11
Identity = 34/54 (62.96%), Postives = 41/54 (75.93%), Query Frame = 1 
Query:   88 QSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDTSSDPMNNF 249
            + GFD CECI  HE+AMRRL+NLLRQSQSYCTDN C Q LPGP  ++ + +  F
Sbjct:    3 EGGFDPCECICSHEHAMRRLINLLRQSQSYCTDNECLQELPGPGASAGEDLTVF 56
[Close]
BLAST of Small integral membrane protein 14 vs. Ensembl Nematostella
Match: EDO47460 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RM84])

HSP 1 Score: 55.0694 bits (131), Expect = 1.514e-10
Identity = 44/99 (44.44%), Postives = 55/99 (55.56%), Query Frame = 1 
Query:   97 FDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDTSSDPMNNFXXXXXXXXXXXAMVGMLLAGYRPGRRNRLP-EKPSRIDQNRNDEDPPP 390
            FD CECI  ++ AMRRL++LLR SQSYCTDN C Q LPGPQ T + P   +    ++ +   A V  LL   RP    + P EKP+  D       P P
Sbjct:   11 FDPCECICSYDGAMRRLISLLRSSQSYCTDNQCLQELPGPQGTDTGP--EYFSTALIMMAWFA-VAFLLYLLRPQSMRQPPDEKPTNNDSGSGSNPPAP 106
[Close]
BLAST of Small integral membrane protein 14 vs. Ensembl Medaka
Match: smim14 (small integral membrane protein 14 [Source:NCBI gene;Acc:101166686])

HSP 1 Score: 59.6918 bits (143), Expect = 7.445e-12
Identity = 50/103 (48.54%), Postives = 58/103 (56.31%), Query Frame = 1 
Query:   88 QSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDTSSDPMNNFXXXXXXXXXXXAMVGMLLAGYRPGR-RNRLPE-KPSRIDQNRNDEDPPP 390
            + GFD CECI  HE AMRRL+NLLRQSQSYCTD  CPQ LPGP         +  L + + L   A+V ++L   RP   R R P  KPS    N   E P P
Sbjct:    3 EGGFDPCECICSHEFAMRRLINLLRQSQSYCTDAECPQELPGPNGLVG---GDGDLTLPMVLMGWAVVALVLFLLRPSSLRGRRPTGKPSGPQNNHGREPPAP 102
[Close]
BLAST of Small integral membrane protein 14 vs. Planmine SMEST
Match: SMESG000040023.1 (SMESG000040023.1)

HSP 1 Score: 176.022 bits (445), Expect = 1.972e-57
Identity = 106/106 (100.00%), Postives = 106/106 (100.00%), Query Frame = 1 
Query:   79 MDDQSGFDACECIYGHENAMXXXXXXXXQSQSYCTDNHCPQTLPGPQDTSSDPMNNFXXXXXXXXXXXAMVGMLLAGYRPGRRNRLPEKPSRIDQNRNDEDPPPIA 396
            MDDQSGFDACECIYGHENAMRRLLNLLRQSQSYCTDNHCPQTLPGPQDTSSDPMNNFGLWIILGLWLLAMVGMLLAGYRPGRRNRLPEKPSRIDQNRNDEDPPPIA
Sbjct:    1 MDDQSGFDACECIYGHENAMRRLLNLLRQSQSYCTDNHCPQTLPGPQDTSSDPMNNFGLWIILGLWLLAMVGMLLAGYRPGRRNRLPEKPSRIDQNRNDEDPPPIA 106
The following BLAST results are available for this feature:
BLAST of Small integral membrane protein 14 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
SMIM145.912e-1143.14small integral membrane protein 14 [Source:HGNC Sy... [more]
SMIM144.240e-1044.44small integral membrane protein 14 [Source:HGNC Sy... [more]
SMIM141.754e-671.05small integral membrane protein 14 [Source:HGNC Sy... [more]
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BLAST of Small integral membrane protein 14 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Small integral membrane protein 14 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Small integral membrane protein 14 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
smim142.273e-967.39small integral membrane protein 14 [Source:ZFIN;Ac... [more]
smim142.273e-967.39small integral membrane protein 14 [Source:ZFIN;Ac... [more]
smim142.353e-967.39small integral membrane protein 14 [Source:ZFIN;Ac... [more]
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BLAST of Small integral membrane protein 14 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
prim21.214e-1044.66DNA primase subunit 2 [Source:Xenbase;Acc:XB-GENE-... [more]
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BLAST of Small integral membrane protein 14 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Smim146.632e-1244.12small integral membrane protein 14 [Source:MGI Sym... [more]
Smim146.632e-1244.12small integral membrane protein 14 [Source:MGI Sym... [more]
Smim141.249e-1147.78small integral membrane protein 14 [Source:MGI Sym... [more]
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BLAST of Small integral membrane protein 14 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q91VT8|SIM14_MOUSE4.639e-1144.12Small integral membrane protein 14 OS=Mus musculus... [more]
sp|Q498C7|SIM14_RAT1.388e-1044.12Small integral membrane protein 14 OS=Rattus norve... [more]
sp|Q96QK8|SIM14_HUMAN2.839e-1043.14Small integral membrane protein 14 OS=Homo sapiens... [more]
sp|Q5RF07|SIM14_PONAB3.289e-1043.14Small integral membrane protein 14 OS=Pongo abelii... [more]
sp|Q2KIK3|SIM14_BOVIN4.433e-942.16Small integral membrane protein 14 OS=Bos taurus O... [more]
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BLAST of Small integral membrane protein 14 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2B4RQ321.323e-1048.48Small integral membrane protein 14 OS=Stylophora p... [more]
A0A1Y1KUW62.976e-1045.71Uncharacterized protein OS=Photinus pyralis OX=705... [more]
E0VG881.145e-942.99Uncharacterized protein OS=Pediculus humanus subsp... [more]
A0A1W7RIU71.412e-946.00Putative C4orf34-like protein OS=Agkistrodon conto... [more]
T1DM562.422e-947.00Putative C4orf34-like protein OS=Crotalus horridus... [more]
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BLAST of Small integral membrane protein 14 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
smim141.767e-1162.96small integral membrane protein 14 [Source:NCBI ge... [more]
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BLAST of Small integral membrane protein 14 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Small integral membrane protein 14 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Small integral membrane protein 14 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO474601.514e-1044.44Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Small integral membrane protein 14 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
smim147.445e-1248.54small integral membrane protein 14 [Source:NCBI ge... [more]
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BLAST of Small integral membrane protein 14 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000040023.11.972e-57100.00SMESG000040023.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016109 ID=SMED30016109|Name=Small integral membrane protein 14|organism=Schmidtea mediterranea sexual|type=transcript|length=538bp
TTATGATTGTTTTTCCTTATCAAATGTAATATGAGTATAATCTATATACG
CTTATAAACCTGTTTGATTAATACTAAAATGGATGATCAAAGTGGATTTG
ATGCGTGTGAATGTATTTATGGTCATGAAAATGCAATGCGTCGATTACTT
AATTTATTAAGACAATCTCAATCATATTGCACAGATAATCATTGTCCACA
AACATTACCGGGTCCTCAAGACACGAGCTCAGATCCTATGAATAATTTTG
GGCTTTGGATTATTTTAGGATTATGGTTACTTGCGATGGTAGGGATGCTG
TTGGCTGGATACCGACCAGGGCGTAGGAATAGACTTCCTGAAAAACCAAG
TAGAATTGACCAAAATAGAAATGATGAGGATCCCCCGCCTATTGCATAAT
TTATGTGTATATCAAAAACTGAACAATTCCTCTAAGTTTCTGGTTATTTC
CTGGATTTTGGTGAAATGTTTTTGCTTATTGTGTTAATCTATATGCTTCT
TCATATTTTGTAATACAAAAAATAATTATTAGAAACAA
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protein sequence of SMED30016109-orf-1

>SMED30016109-orf-1 ID=SMED30016109-orf-1|Name=SMED30016109-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=107bp
MDDQSGFDACECIYGHENAMRRLLNLLRQSQSYCTDNHCPQTLPGPQDTS
SDPMNNFGLWIILGLWLLAMVGMLLAGYRPGRRNRLPEKPSRIDQNRNDE
DPPPIA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR020309Uncharacterised_CD034/YQF4
Vocabulary: molecular function
TermDefinition
GO:0003674molecular_function
Vocabulary: cellular component
TermDefinition
GO:0005783endoplasmic reticulum
GO:0005789endoplasmic reticulum membrane
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: biological process
TermDefinition
GO:0008150biological_process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020309Uncharacterised protein family, CD034/YQF4PFAMPF11027DUF2615coord: 4..104
e-value: 9.5E-27
score: 93.2
IPR020309Uncharacterised protein family, CD034/YQF4PANTHERPTHR31019UNCHARACTERIZEDcoord: 4..104
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 84..99
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 84..106
NoneNo IPR availablePRODOMPD288703coord: 4..103
e-value: 2.0E-9
score: 144.0
NoneNo IPR availableTMHMMTMhelixcoord: 56..78