PH domain-containing protein

Overview
NamePH domain-containing protein
Smed IDSMED30016107
Length (bp)1584
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of PH domain-containing protein (SMED30016107) t-SNE clustered cells

Violin plots show distribution of expression levels for PH domain-containing protein (SMED30016107) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of PH domain-containing protein (SMED30016107) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for PH domain-containing protein (SMED30016107) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30016107

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30016107SMESG000007865.1 Contig6613GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
Smed sexual biotypeSMED30016107SMESG000077556.1 SMESG000073618.1 SMESG000068262.1 SMESG000067334.1 SMESG000060260.1 SMESG000050479.1 SMESG000044971.1 SMESG000043973.1 SMESG000039741.1 SMESG000039356.1 SMESG000033763.1 SMESG000021640.1 SMESG000021522.1 SMESG000018320.1 SMESG000012464.1 SMESG000007865.1 SMESG000007864.1 SMESG000007021.1 Contig20246newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016107SMESG000077556.1 SMESG000073618.1 SMESG000068262.1 SMESG000067334.1 SMESG000060260.1 SMESG000050479.1 SMESG000044971.1 SMESG000043973.1 SMESG000039741.1 SMESG000039356.1 SMESG000033763.1 SMESG000021640.1 SMESG000021522.1 SMESG000018320.1 SMESG000012464.1 SMESG000007865.1 SMESG000007864.1 SMESG000007021.1 Contig20246uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016107SMESG000007865.1 Contig48091newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016107SMESG000007865.1 Contig48091uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
protonephridiaSMED30016107SMESG000007865.1 dd_Smed_v4_3737_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016107SMESG000007865.1 dd_Smed_v4_3737_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30016107SMESG000007865.1 dd_Smed_v4_3737_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of PH domain-containing protein vs. Ensembl Human
Match: PLEKHD1 (pleckstrin homology and coiled-coil domain containing D1 [Source:HGNC Symbol;Acc:HGNC:20148])

HSP 1 Score: 71.633 bits (174), Expect = 1.255e-12
Identity = 37/138 (26.81%), Postives = 68/138 (49.28%), Query Frame = 1 
Query:  145 GFLLKRS-DQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDLMQTTSTTKYARVKQQFSD 555
            G L KR   + + KW  RF I+K+++ L+Y E E    + +KYFN  P+GVI L  C++    + S      I +  F   ++ + +++  +  W+E L  +  + + N    +  +K L       + A+ KQ++ D
Sbjct:   33 GVLWKRPFGRPSAKWSRRFFIIKESFLLYYSESEKKSFETNKYFNIHPKGVIPLGGCLVEPKEEPSMPYAMKISHQDFHGNILLAAESEFEQTQWLEMLQESGKVTWKNAQLGEAMIKSL--EAQGLQLAKEKQEYLD 168
[Close]
BLAST of PH domain-containing protein vs. Ensembl Celegans
Match: F31D4.5 (Pleckstrin homology domain-containing family D member 1 [Source:UniProtKB/Swiss-Prot;Acc:O45420])

HSP 1 Score: 56.9954 bits (136), Expect = 2.455e-8
Identity = 26/121 (21.49%), Postives = 60/121 (49.59%), Query Frame = 1 
Query:  145 GFLLKRSDQQ--TNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDL 501
            G L+K+  ++  + +W  RF +LK+ + ++Y        +K++  +  P+G+I L  C I    D  +  C +I +      ++ +  +   +E W++ L   + I+Y N +  +  +++L
Sbjct:   88 GILMKKYKRKNRSARWAKRFFVLKECFLIYYSTSYKKVFEKTRRIDLHPKGIIPLIGCSIVSGGDVDKKNCLLIAHPQLPSAIIVAASDHQTQEMWLKALRSATKISYKNTVVGETMIREL 208
[Close]
BLAST of PH domain-containing protein vs. Ensembl Celegans
Match: F31D4.5 (Pleckstrin homology domain-containing family D member 1 [Source:UniProtKB/Swiss-Prot;Acc:O45420])

HSP 1 Score: 56.6102 bits (135), Expect = 2.725e-8
Identity = 26/121 (21.49%), Postives = 60/121 (49.59%), Query Frame = 1 
Query:  145 GFLLKRSDQQ--TNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDL 501
            G L+K+  ++  + +W  RF +LK+ + ++Y        +K++  +  P+G+I L  C I    D  +  C +I +      ++ +  +   +E W++ L   + I+Y N +  +  +++L
Sbjct:   88 GILMKKYKRKNRSARWAKRFFVLKECFLIYYSTSYKKVFEKTRRIDLHPKGIIPLIGCSIVSGGDVDKKNCLLIAHPQLPSAIIVAASDHQTQEMWLKALRSATKISYKNTVVGETMIREL 208
[Close]
BLAST of PH domain-containing protein vs. Ensembl Zebrafish
Match: plekhd1 (pleckstrin homology domain containing, family D (with coiled-coil domains) member 1 [Source:ZFIN;Acc:ZDB-GENE-130926-1])

HSP 1 Score: 72.4034 bits (176), Expect = 3.247e-13
Identity = 38/138 (27.54%), Postives = 68/138 (49.28%), Query Frame = 1 
Query:  145 GFLLKRS-DQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDLMQTTSTTKYARVKQQFSD 555
            G L KR   + + KW  RF I+K+++ L+Y E+E    + +KYFN  P+GVI L  CI+    D       +I +  F   +V + ++   +  W+E L  +  + + N    +  ++ L       + A+ KQ++ D
Sbjct:   34 GVLWKRPFGRPSAKWSRRFFIIKESFLLYYAENEKKSFETNKYFNIHPKGVIPLGGCIVEAREDQGMPFAMVINHEDFTGNIVLAAESVSEQSQWVEMLQESGKVTWKNAQLGEAMIESL--EAQGLQLAKEKQEYLD 169
[Close]
BLAST of PH domain-containing protein vs. Ensembl Zebrafish
Match: plekhd1 (pleckstrin homology domain containing, family D (with coiled-coil domains) member 1 [Source:ZFIN;Acc:ZDB-GENE-130926-1])

HSP 1 Score: 70.0922 bits (170), Expect = 3.888e-13
Identity = 39/151 (25.83%), Postives = 72/151 (47.68%), Query Frame = 1 
Query:  106 SGGFELDKHAEKSGFLLKRS-DQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDLMQTTSTTKYARVKQQFSD 555
            S   ++    +  G L KR   + + KW  RF I+K+++ L+Y E+E    + +KYFN  P+GVI L  CI+    D       +I +  F   +V + ++   +  W+E L  +  + + N    +  ++ L       + A+ KQ++ D
Sbjct:   34 SDALDISTKVQLHGVLWKRPFGRPSAKWSRRFFIIKESFLLYYAENEKKSFETNKYFNIHPKGVIPLGGCIVEAREDQGMPFAMVINHEDFTGNIVLAAESVSEQSQWVEMLQESGKVTWKNAQLGEAMIESL--EAQGLQLAKEKQEYLD 182
[Close]
BLAST of PH domain-containing protein vs. Ensembl Zebrafish
Match: plekhd1 (pleckstrin homology domain containing, family D (with coiled-coil domains) member 1 [Source:ZFIN;Acc:ZDB-GENE-130926-1])

HSP 1 Score: 71.633 bits (174), Expect = 4.791e-13
Identity = 39/151 (25.83%), Postives = 72/151 (47.68%), Query Frame = 1 
Query:  106 SGGFELDKHAEKSGFLLKRS-DQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDLMQTTSTTKYARVKQQFSD 555
            S   ++    +  G L KR   + + KW  RF I+K+++ L+Y E+E    + +KYFN  P+GVI L  CI+    D       +I +  F   +V + ++   +  W+E L  +  + + N    +  ++ L       + A+ KQ++ D
Sbjct:   21 SDALDISTKVQLHGVLWKRPFGRPSAKWSRRFFIIKESFLLYYAENEKKSFETNKYFNIHPKGVIPLGGCIVEAREDQGMPFAMVINHEDFTGNIVLAAESVSEQSQWVEMLQESGKVTWKNAQLGEAMIESL--EAQGLQLAKEKQEYLD 169
[Close]
BLAST of PH domain-containing protein vs. Ensembl Zebrafish
Match: plekhd1 (pleckstrin homology domain containing, family D (with coiled-coil domains) member 1 [Source:ZFIN;Acc:ZDB-GENE-130926-1])

HSP 1 Score: 71.633 bits (174), Expect = 5.499e-13
Identity = 38/138 (27.54%), Postives = 68/138 (49.28%), Query Frame = 1 
Query:  145 GFLLKRS-DQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDLMQTTSTTKYARVKQQFSD 555
            G L KR   + + KW  RF I+K+++ L+Y E+E    + +KYFN  P+GVI L  CI+    D       +I +  F   +V + ++   +  W+E L  +  + + N    +  ++ L       + A+ KQ++ D
Sbjct:   34 GVLWKRPFGRPSAKWSRRFFIIKESFLLYYAENEKKSFETNKYFNIHPKGVIPLGGCIVEAREDQGMPFAMVINHEDFTGNIVLAAESVSEQSQWVEMLQESGKVTWKNAQLGEAMIESL--EAQGLQLAKEKQEYLD 169
[Close]
BLAST of PH domain-containing protein vs. Ensembl Zebrafish
Match: plekhd1 (pleckstrin homology domain containing, family D (with coiled-coil domains) member 1 [Source:ZFIN;Acc:ZDB-GENE-130926-1])

HSP 1 Score: 71.633 bits (174), Expect = 6.312e-13
Identity = 38/138 (27.54%), Postives = 68/138 (49.28%), Query Frame = 1 
Query:  145 GFLLKRS-DQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDLMQTTSTTKYARVKQQFSD 555
            G L KR   + + KW  RF I+K+++ L+Y E+E    + +KYFN  P+GVI L  CI+    D       +I +  F   +V + ++   +  W+E L  +  + + N    +  ++ L       + A+ KQ++ D
Sbjct:   34 GVLWKRPFGRPSAKWSRRFFIIKESFLLYYAENEKKSFETNKYFNIHPKGVIPLGGCIVEAREDQGMPFAMVINHEDFTGNIVLAAESVSEQSQWVEMLQESGKVTWKNAQLGEAMIESL--EAQGLQLAKEKQEYLD 169
[Close]
BLAST of PH domain-containing protein vs. Ensembl Xenopus
Match: PLEKHD1 (pleckstrin homology and coiled-coil domain containing D1 [Source:NCBI gene;Acc:100485012])

HSP 1 Score: 83.9593 bits (206), Expect = 1.440e-16
Identity = 103/394 (26.14%), Postives = 188/394 (47.72%), Query Frame = 1 
Query:  115 FELDKHAEKSGFLLKRS-DQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHN------MLET------------QKFVKDLMQTTSTTKYARVKQQFSDVKDENQVLIXXXXXXXXXXXXXXQFIKDLQLEFSKFKEEMDSAYLVLNTLXXXXXXXXXXXXXXXXXXSNLECERLETSRLLSATCSK--------EDEILSLQKKLDEINSLRDKIDQDRENIKKQLAEKEDISIYLERENKRIVNQAQEXXXXXXXXXXXXXDVERXXXXXXXXXXXXXXXXXXXXMSLGRLHDNIVEGGTKYTNEKKTEMTSDVNKLKDYFESVI 1215
            F++    +  G L K+   +Q+ KW  RF I+KD++ L+Y E+E    + ++YFN  P+GVI L  C++    +       +I +  F   +V + +++L    W+E L  +  + + N      M+E+            Q++   LM+   T +    ++Q  +++  NQ+L       E E    E+ +++L+LE  K K+++D     L  + + K  L      L+  +  L  E+  T  +L AT S         E     LQ  L +I S   K+ +++   +K++ E E+    LE E    V+Q+Q L   + +L  +    ER+L+++   +++ E+RLREAE +L RL   +         +K+ +M +DV+ LK +FE  I
Sbjct:   23 FDIKTKVQLQGVLWKKPFGRQSAKWSKRFFIIKDSFLLYYAENERKNFESNRYFNIHPKGVIPLGGCMVNAKEEPHMPYVIVISHEDFHGSIVLAAESELEHSQWLEMLHESGKVTWQNAQLGEAMIESLEAQGLQLAKERQEYFDKLME--ETEELCSQREQKEELERLNQIL-------ESEKSHFEELVQELRLEQEKIKKDLDITVQCLKGVGEEKQELHCLTQHLQKTMEELSVEKQRTLAILEATKSHVPAPTNHSELPPQGLQSNLQQIESKMQKLLEEKLQAEKRMKENEEKFRALEEERTFYVSQSQALQQSLTELTAEKEQKERELKVQLKVQMDLEKRLREAEEALKRLELEL--NSAVINGDKEEKMRADVSHLKKFFEECI 405
[Close]
BLAST of PH domain-containing protein vs. Ensembl Xenopus
Match: PLEKHD1 (pleckstrin homology and coiled-coil domain containing D1 [Source:NCBI gene;Acc:100485012])

HSP 1 Score: 80.1073 bits (196), Expect = 2.137e-15
Identity = 99/394 (25.13%), Postives = 180/394 (45.69%), Query Frame = 1 
Query:  115 FELDKHAEKSGFLLKRS-DQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHN------MLET------------QKFVKDLMQTTSTTKYARVKQQFSDVKDENQVLIXXXXXXXXXXXXXXQFIKDLQLEFSKFKEEMDSAYLVLNTLXXXXXXXXXXXXXXXXXXSNLECERLETSRLLSATCSK--------EDEILSLQKKLDEINSLRDKIDQDRENIKKQLAEKEDISIYLERENKRIVNQAQEXXXXXXXXXXXXXDVERXXXXXXXXXXXXXXXXXXXXMSLGRLHDNIVEGGTKYTNEKKTEMTSDVNKLKDYFESVI 1215
            F++    +  G L K+   +Q+ KW  RF I+KD++ L+Y E+E    + ++YFN  P+GVI L  C++    +       +I +  F   +V + +++L    W+E L  +  + + N      M+E+            Q++   LM+   T +    ++Q  +++  NQ+L       E E    E+ +++L+LE  K K+++D     L  + + K  L      L+  +  L  E+  T  +L AT S         E     LQ  L +I S   K+ +++   +K++ E E+    LE E    V+Q+Q L   + +L  +    ER+L++ K      +R     E +L RL   +         +K+ +M +DV+ LK +FE  I
Sbjct:   23 FDIKTKVQLQGVLWKKPFGRQSAKWSKRFFIIKDSFLLYYAENERKNFESNRYFNIHPKGVIPLGGCMVNAKEEPHMPYVIVISHEDFHGSIVLAAESELEHSQWLEMLHESGKVTWQNAQLGEAMIESLEAQGLQLAKERQEYFDKLME--ETEELCSQREQKEELERLNQIL-------ESEKSHFEELVQELRLEQEKIKKDLDITVQCLKGVGEEKQELHCLTQHLQKTMEELSVEKQRTLAILEATKSHVPAPTNHSELPPQGLQSNLQQIESKMQKLLEEKLQAEKRMKENEEKFRALEEERTFYVSQSQALQQSLTELTAEKEQKERELKVLKVQMDLEKRLREAEE-ALKRLELEL--NSAVINGDKEEKMRADVSHLKKFFEECI 404
[Close]
BLAST of PH domain-containing protein vs. Ensembl Mouse
Match: Plekhd1 (pleckstrin homology domain containing, family D (with coiled-coil domains) member 1 [Source:MGI Symbol;Acc:MGI:3036228])

HSP 1 Score: 71.2478 bits (173), Expect = 1.233e-12
Identity = 37/138 (26.81%), Postives = 68/138 (49.28%), Query Frame = 1 
Query:  145 GFLLKRS-DQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDLMQTTSTTKYARVKQQFSD 555
            G L KR   + + KW  RF I+K+++ L+Y E E    + +KYFN  P+GVI L  C++    + S      I +  F   ++ + +++  +  W+E L  +  + + N    +  +K L       + A+ KQ++ D
Sbjct:   33 GVLWKRPFGRSSAKWSRRFFIIKESFLLYYSESERKSFETNKYFNIHPKGVIPLGGCLVEAREEPSMPYAMKISHQDFHGNVLLAAESEFEQTQWLEMLQESGKVTWKNAQLGEAMIKSL--EAQGLQLAKEKQEYLD 168
[Close]
BLAST of PH domain-containing protein vs. UniProt/SwissProt
Match: sp|A6NEE1|PLHD1_HUMAN (Pleckstrin homology domain-containing family D member 1 OS=Homo sapiens OX=9606 GN=PLEKHD1 PE=2 SV=3)

HSP 1 Score: 71.633 bits (174), Expect = 6.027e-12
Identity = 37/138 (26.81%), Postives = 68/138 (49.28%), Query Frame = 1 
Query:  145 GFLLKRS-DQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDLMQTTSTTKYARVKQQFSD 555
            G L KR   + + KW  RF I+K+++ L+Y E E    + +KYFN  P+GVI L  C++    + S      I +  F   ++ + +++  +  W+E L  +  + + N    +  +K L       + A+ KQ++ D
Sbjct:   33 GVLWKRPFGRPSAKWSRRFFIIKESFLLYYSESEKKSFETNKYFNIHPKGVIPLGGCLVEPKEEPSMPYAMKISHQDFHGNILLAAESEFEQTQWLEMLQESGKVTWKNAQLGEAMIKSL--EAQGLQLAKEKQEYLD 168
[Close]
BLAST of PH domain-containing protein vs. UniProt/SwissProt
Match: sp|B2RPU2|PLHD1_MOUSE (Pleckstrin homology domain-containing family D member 1 OS=Mus musculus OX=10090 GN=Plekhd1 PE=1 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 8.627e-12
Identity = 37/138 (26.81%), Postives = 68/138 (49.28%), Query Frame = 1 
Query:  145 GFLLKRS-DQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDLMQTTSTTKYARVKQQFSD 555
            G L KR   + + KW  RF I+K+++ L+Y E E    + +KYFN  P+GVI L  C++    + S      I +  F   ++ + +++  +  W+E L  +  + + N    +  +K L       + A+ KQ++ D
Sbjct:   33 GVLWKRPFGRSSAKWSRRFFIIKESFLLYYSESERKSFETNKYFNIHPKGVIPLGGCLVEAREEPSMPYAMKISHQDFHGNVLLAAESEFEQTQWLEMLQESGKVTWKNAQLGEAMIKSL--EAQGLQLAKEKQEYLD 168
[Close]
BLAST of PH domain-containing protein vs. UniProt/SwissProt
Match: sp|B1WBU8|PLHD1_RAT (Pleckstrin homology domain-containing family D member 1 OS=Rattus norvegicus OX=10116 GN=Plekhd1 PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 8.936e-12
Identity = 37/138 (26.81%), Postives = 68/138 (49.28%), Query Frame = 1 
Query:  145 GFLLKRS-DQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDLMQTTSTTKYARVKQQFSD 555
            G L KR   + + KW  RF I+K+++ L+Y E E    + +KYFN  P+GVI L  C++    + S      I +  F   ++ + +++  +  W+E L  +  + + N    +  +K L       + A+ KQ++ D
Sbjct:   33 GVLWKRPFGRSSAKWSRRFFIIKESFLLYYSESERKSFETNKYFNIHPKGVIPLGGCLVEAREEPSMPYAMKISHQDFHGNVLLAAESEFEQTQWLEMLQESGKVTWKNAQLGEAMIKSL--EAQGLQLAKEKQEYLD 168
[Close]
BLAST of PH domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9ST43|PH1_ARATH (Pleckstrin homology domain-containing protein 1 OS=Arabidopsis thaliana OX=3702 GN=PH1 PE=2 SV=2)

HSP 1 Score: 58.5362 bits (140), Expect = 5.145e-9
Identity = 38/129 (29.46%), Postives = 62/129 (48.06%), Query Frame = 1 
Query:  112 GFELDKHAEKSGFLLKRSDQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKD 498
            G E   + E+SG+L K+ D     W+ R+ +LK    LW+K+  A  ++ S      P+GVI + DC+  +  +   N  F  E ++    + F  DN+  +E WI ++    SI  H+   T   V D
Sbjct:   20 GIEFWSNPERSGWLTKQGDY-IKTWRRRWFVLKRGKLLWFKDQAAAGIRGST-----PRGVISVGDCLTVKGAEDVVNKPFAFELSSGSYTMFFIADNEKEKEEWINSI--GRSIVQHSRSVTDSEVLD 140
[Close]
BLAST of PH domain-containing protein vs. UniProt/SwissProt
Match: sp|O45420|PLHD1_CAEEL (Pleckstrin homology domain-containing family D member 1 OS=Caenorhabditis elegans OX=6239 GN=F31D4.5 PE=3 SV=2)

HSP 1 Score: 56.6102 bits (135), Expect = 3.466e-7
Identity = 26/121 (21.49%), Postives = 60/121 (49.59%), Query Frame = 1 
Query:  145 GFLLKRSDQQ--TNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDL 501
            G L+K+  ++  + +W  RF +LK+ + ++Y        +K++  +  P+G+I L  C I    D  +  C +I +      ++ +  +   +E W++ L   + I+Y N +  +  +++L
Sbjct:   88 GILMKKYKRKNRSARWAKRFFVLKECFLIYYSTSYKKVFEKTRRIDLHPKGIIPLIGCSIVSGGDVDKKNCLLIAHPQLPSAIIVAASDHQTQEMWLKALRSATKISYKNTVVGETMIREL 208
[Close]
BLAST of PH domain-containing protein vs. TrEMBL
Match: A0A267GRL2 (PH domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig018124g1 PE=4 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 2.454e-22
Identity = 131/465 (28.17%), Postives = 212/465 (45.59%), Query Frame = 1 
Query:  115 FELDKHAEKSGFLLKRSDQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFV-----KDLMQTTSTTKYARVKQQFSDVKDENQVLIXXXXXXXXXXXXXXQF----IKDLQLEFSKFKEEMDSAYLVLNTLXXXXXXXXXXXXXXXXXXSNLECERLETSRLLSATCSKEDEILSLQKKLDEINSLRDKIDQD---------RENIKKQLAEKEDISIYLERENKRIVNQAQEXXXXXXXXXXXXXDVERXXXXXXXXXXXXXXXXXXXXMSLGRLHDNIVEGGTKYTNEKKTEMTSDVNKLKDYFESVINSQSTSPKGVDENHATRGRSSSKSRVLNSRKNSIDVDKFDKNSIDNQTKLSLQMPKSYRDSIRVREESQKLREEHRKRIS 1455
            F LD   +  GFLLKRS   +NKWQ RFCI+K+ + L +KE EA ++K+S ++N K    I L DC+I  + D++  C   I         +FS +N+  ++ W   L   S I + N   + K +     + + + T + +Y ++ ++  +++          E  + E ++++++    +++L+ E +  + EM  A   L                             E +R   A   K  + L  Q+K        D+  QD         R  +++++ EK  ++  LE E      QAQEL+N + +L  Q      +L  E+ AR  AE RLREAE SL RLH ++ E  + YT E+K EM +DV +LK YFE++I S+ST    V    ++R                          +  Q +  LQ P+  +    VR  SQ+LRE HR R+S
Sbjct:   14 FHLDNRVQYHGFLLKRS-HLSNKWQRRFCIIKEGFLLVFKEQEADRIKRSSFYNVKVNAAIPLGDCVINLVSDATVQCGIEINIFVPSVRFLFSCENRSIQQDWYSALSDASRITHANFQISDKLIATLESQGMEKVTQSERYRKMLEEEVELRHNESEKASRLEAQQTELQQEKEYAELAMRELESELAALRSEMTDAQTTL-----------------------------ERTREEKAALEKRAKQLLEQEK----QRAGDEESQDNSGEELAKLRREVEEKIREKSALASALEGERSAFDQQAQELSNAVRQLSEQRARASAELAAEQRARQLAEARLREAENSLARLHASVSEERSVYTPEQKEEMKADVGQLKAYFENLIASKSTDSGAVKLRRSSR--------------------------LQQQQQQVLQQPEQQQQPFLVRARSQRLRERHRSRLS 418
[Close]
BLAST of PH domain-containing protein vs. TrEMBL
Match: A0A1I8I260 (PH domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 5.731e-16
Identity = 47/129 (36.43%), Postives = 69/129 (53.49%), Query Frame = 1 
Query:  115 FELDKHAEKSGFLLKRSDQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDL 501
            F LD   +  GFLLKRS   +NKWQ RFCI+K+ + L +KE EA ++K+S ++N K    I L DC+I  I D++  C   I         +FS +N+  ++ W   L   S I + N   + K +  L
Sbjct:   12 FHLDNKVQHHGFLLKRS-HLSNKWQRRFCIIKEGFLLVFKEQEAERIKRSSFYNVKANAAIPLGDCVINLISDATVQCGIDINIFVPSVRFLFSCENRSNQQDWYSALSDASRITHANFQISDKLIATL 139
[Close]
BLAST of PH domain-containing protein vs. TrEMBL
Match: A0A1I8I492 (PH domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.266e-15
Identity = 47/129 (36.43%), Postives = 69/129 (53.49%), Query Frame = 1 
Query:  115 FELDKHAEKSGFLLKRSDQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDL 501
            F LD   +  GFLLKRS   +NKWQ RFCI+K+ + L +KE EA ++K+S ++N K    I L DC+I  I D++  C   I         +FS +N+  ++ W   L   S I + N   + K +  L
Sbjct:   12 FHLDNKVQHHGFLLKRS-HLSNKWQRRFCIIKEGFLLVFKEQEAERIKRSSFYNVKVNAAIPLGDCVINLISDATVQCGIDINIFVPSVRFLFSCENRSNQQDWYSALSDASRITHANFQISDKLIATL 139
[Close]
BLAST of PH domain-containing protein vs. TrEMBL
Match: A0A3S0ZSH2 (PH domain-containing protein OS=Elysia chlorotica OX=188477 GN=EGW08_007486 PE=4 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 1.291e-15
Identity = 115/414 (27.78%), Postives = 207/414 (50.00%), Query Frame = 1 
Query:  145 GFLLKRS-DQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDL------MQTTSTTKYARVKQQFSDVKDE---NQVLIXXXXXXXXXXXXXXQFIKDLQLEFSKFKEEMDSAYLVLNTLXXXXXXXXXXXXXXXXXXSNLECERLETSRLLS---------ATCSKEDEILS-----LQKKLDEINSLRDKIDQDRENIKKQLAEKEDISIYLERENKRIVNQAQEXXXXXXXXXXXXXDVERXXXXXXXXXXXXXXXXXXXXMSLGRLHDNIVEGGTKYTNEKKTEMTSDVNKLKDYFESVIN-SQSTSPKGVDENHATRGRSSSKSRVLNSRKN 1311
            G LLKR    Q+ KW  RF ++KD + ++Y  +E    ++ +YFN  P+GV+ L +C  +   +  Q  CF+IE+      LV + D+   R+ W+E L  +  + + N     + ++ L      M         R++ +   + +E    + L K+  +LEKE E+ E F K++Q E  + KEE+D    +++ +E  +  L +  ++ +  L +L  ++    R+LS          T S+E + LS     LQK+L+EI+S   + +  R + + +L + ++    LE E + I   A EL + I+ L +Q    E++L  E  AR+ AE+RL+EAE SL  + + ++    +  ++ K +M  +V KLK++FE +   S+ +S + +   H    R +   R    R N
Sbjct:   28 GVLLKRPFAHQSTKWTKRFFLVKDGFLMYYDANEKKDYERREYFNIHPKGVLPLGECSFKACKEPQQPFCFLIESPEIDGKLVLAADSAYERDQWLEILESSRRVTWKNSQLGDEMIRRLEDQGLQMAIEKQDYIDRLQSEVMALTEERLKTEELEKVNLELEKEKEKLELFTKEMQDEHDRLKEELDETAFIMHQVESDRVKLYESLTEQQQQLESLHQDK---QRILSELHASVEEQTTLSQEKQSLSQRSEELQKQLEEIDSKTRQAEDIRSSAEDRLKDNQERLDQLEEEKQAISQHAHELESTIHDLVSQKEMTEKELREEIKARMSAEQRLQEAEASLTCIDNAVIAQSHRLDSDAKEDMAVNVKKLKNFFEDLAQESKISSEQPLIIRHGMMARKTLARRAKTVRYN 438
[Close]
BLAST of PH domain-containing protein vs. TrEMBL
Match: A0A267E9Z7 (PH domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000389g1 PE=4 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.489e-15
Identity = 47/129 (36.43%), Postives = 69/129 (53.49%), Query Frame = 1 
Query:  115 FELDKHAEKSGFLLKRSDQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDL 501
            F LD   +  GFLLKRS   +NKWQ RFCI+K+ + L +KE EA ++K+S ++N K    I L DC+I  I D++  C   I         +FS +N+  ++ W   L   S I + N   + K +  L
Sbjct:   16 FHLDNKVQHHGFLLKRS-HLSNKWQRRFCIIKEGFLLVFKEQEAERIKRSSFYNVKVNAAIPLGDCVINLISDATVQCGIDINIFVPSVRFLFSCENRSNQQDWYSALSDASRITHANFQISDKLIATL 143
[Close]
BLAST of PH domain-containing protein vs. Ensembl Medaka
Match: plekhd1 (solute carrier family 39 member 9 [Source:NCBI gene;Acc:101172541])

HSP 1 Score: 69.3218 bits (168), Expect = 3.821e-12
Identity = 41/164 (25.00%), Postives = 80/164 (48.78%), Query Frame = 1 
Query:  145 GFLLKRS-DQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHN------MLET------------QKFVKDLMQTTSTTKYARVKQQFSDVKDENQVL 579
            G L KR   + + KW  RF I+KD++ L+Y E+E    + +++FN  P+GVI L  C++    D       ++    F   +V + +++  +  W+E L  +  + + N      M+E+            Q+++  LM+ T    + R +++  +++  NQVL
Sbjct:   44 GVLWKRPFGRPSAKWSRRFFIIKDSFLLYYGENEKKSFESNRFFNIHPKGVIPLGGCVVTANEDMGMPFAIVVNLEDFTGAVVLAAESEAEQLQWMEMLQESGKVTWKNAQLGEAMIESLEAQGLQLAKEKQEYLDKLMEETEELSHQRAQRE--ELERLNQVL 205
[Close]
BLAST of PH domain-containing protein vs. Planmine SMEST
Match: SMESG000007865.1 (SMESG000007865.1)

HSP 1 Score: 792.727 bits (2046), Expect = 0.000e+0
Identity = 463/464 (99.78%), Postives = 464/464 (100.00%), Query Frame = 1 
Query:   88 MSINIHSGGFELDKHAEKSGFLLKRSDQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDLMQTTSTTKYARVKQQFSDVKDENQVLIXXXXXXXXXXXXXXQFIKDLQLEFSKFKEEMDSAYLVLNTLXXXXXXXXXXXXXXXXXXSNLECERLETSRLLSATCSKEDEILSLQKKLDEINSLRDKIDQDRENIKKQLAEKEDISIYLERENKRIVNQAQEXXXXXXXXXXXXXDVERXXXXXXXXXXXXXXXXXXXXMSLGRLHDNIVEGGTKYTNEKKTEMTSDVNKLKDYFESVINSQSTSPKGVDENHATRGRSSSKSRVLNSRKNSIDVDKFDKNSIDNQTKLSLQMPKSYRDSIRVREESQKLREEHRKRISQIQWADAD 1479
            MSINIHSGGFELDKHAEKSGFLLKRSDQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDLMQTTSTTKYARVKQQFSDVKDENQVLIKMKEQLEKENEEKEQFIKDLQLEFSKFKEEMDSAYLVLNTLEQSKSNLEKKNSKLENDLSNLECERLETSRLLSATCSKEDEILSLQKKLDEINSLRDKIDQDRENIKKQLAEKEDISIYLERENKRIVNQAQELNNLINKLKNQNIDVERKLELEKAARIEAERRLREAEMSLGRLHD+IVEGGTKYTNEKKTEMTSDVNKLKDYFESVINSQSTSPKGVDENHATRGRSSSKSRVLNSRKNSIDVDKFDKNSIDNQTKLSLQMPKSYRDSIRVREESQKLREEHRKRISQIQWADAD
Sbjct:    1 MSINIHSGGFELDKHAEKSGFLLKRSDQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDLMQTTSTTKYARVKQQFSDVKDENQVLIKMKEQLEKENEEKEQFIKDLQLEFSKFKEEMDSAYLVLNTLEQSKSNLEKKNSKLENDLSNLECERLETSRLLSATCSKEDEILSLQKKLDEINSLRDKIDQDRENIKKQLAEKEDISIYLERENKRIVNQAQELNNLINKLKNQNIDVERKLELEKAARIEAERRLREAEMSLGRLHDSIVEGGTKYTNEKKTEMTSDVNKLKDYFESVINSQSTSPKGVDENHATRGRSSSKSRVLNSRKNSIDVDKFDKNSIDNQTKLSLQMPKSYRDSIRVREESQKLREEHRKRISQIQWADAD 464
[Close]
BLAST of PH domain-containing protein vs. Planmine SMEST
Match: SMESG000007865.1 (SMESG000007865.1)

HSP 1 Score: 778.474 bits (2009), Expect = 0.000e+0
Identity = 457/459 (99.56%), Postives = 458/459 (99.78%), Query Frame = 1 
Query:   88 MSINIHSGGFELDKHAEKSGFLLKRSDQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDLMQTTSTTKYARVKQQFSDVKDENQVLIXXXXXXXXXXXXXXQFIKDLQLEFSKFKEEMDSAYLVLNTLXXXXXXXXXXXXXXXXXXSNLECERLETSRLLSATCSKEDEILSLQKKLDEINSLRDKIDQDRENIKKQLAEKEDISIYLERENKRIVNQAQEXXXXXXXXXXXXXDVERXXXXXXXXXXXXXXXXXXXXMSLGRLHDNIVEGGTKYTNEKKTEMTSDVNKLKDYFESVINSQSTSPKGVDENHATRGRSSSKSRVLNSRKNSIDVDKFDKNSIDNQTKLSLQMPKSYRDSIRVREESQKLREEHRKRISQIQ 1464
            MSINIHSGGFELDKHAEKSGFLLKRSDQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDLMQTTSTTKYARVKQQFSDVKDENQVLIKMKEQLEKENEEKEQFIKDLQLEFSKFKEEMDSAYLVLNTLEQSKSNLEKKNSKLENDLSNLECERLETSRLLSATCSKEDEILSLQKKLDEINSLRDKIDQDRENIKKQLAEKEDISIYLERENKRIVNQAQELNNLINKLKNQNIDVERKLELEKAARIEAERRLREAEMSLGRLHD+IVEGGTKYTNEKKTEMTSDVNKLKDYFESVINSQSTSPKGVDENHATRGRSSSKSRVLNSRKNSIDVDKFDKNSIDNQTKLSLQMPKSYRDSIRVREESQKLREEHRKRISQI 
Sbjct:    1 MSINIHSGGFELDKHAEKSGFLLKRSDQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDLMQTTSTTKYARVKQQFSDVKDENQVLIKMKEQLEKENEEKEQFIKDLQLEFSKFKEEMDSAYLVLNTLEQSKSNLEKKNSKLENDLSNLECERLETSRLLSATCSKEDEILSLQKKLDEINSLRDKIDQDRENIKKQLAEKEDISIYLERENKRIVNQAQELNNLINKLKNQNIDVERKLELEKAARIEAERRLREAEMSLGRLHDSIVEGGTKYTNEKKTEMTSDVNKLKDYFESVINSQSTSPKGVDENHATRGRSSSKSRVLNSRKNSIDVDKFDKNSIDNQTKLSLQMPKSYRDSIRVREESQKLREEHRKRISQIH 459
[Close]
BLAST of PH domain-containing protein vs. Planmine SMEST
Match: SMESG000007865.1 (SMESG000007865.1)

HSP 1 Score: 536.569 bits (1381), Expect = 0.000e+0
Identity = 299/299 (100.00%), Postives = 299/299 (100.00%), Query Frame = 1 
Query:   88 MSINIHSGGFELDKHAEKSGFLLKRSDQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDLMQTTSTTKYARVKQQFSDVKDENQVLIXXXXXXXXXXXXXXQFIKDLQLEFSKFKEEMDSAYLVLNTLXXXXXXXXXXXXXXXXXXSNLECERLETSRLLSATCSKEDEILSLQKKLDEINSLRDKIDQDRENIKKQLAEKEDISIYLERENKRIVNQAQE 984
            MSINIHSGGFELDKHAEKSGFLLKRSDQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDLMQTTSTTKYARVKQQFSDVKDENQVLIKMKEQLEKENEEKEQFIKDLQLEFSKFKEEMDSAYLVLNTLEQSKSNLEKKNSKLENDLSNLECERLETSRLLSATCSKEDEILSLQKKLDEINSLRDKIDQDRENIKKQLAEKEDISIYLERENKRIVNQAQE
Sbjct:    1 MSINIHSGGFELDKHAEKSGFLLKRSDQQTNKWQNRFCILKDNYFLWYKEDEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPLVFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDLMQTTSTTKYARVKQQFSDVKDENQVLIKMKEQLEKENEEKEQFIKDLQLEFSKFKEEMDSAYLVLNTLEQSKSNLEKKNSKLENDLSNLECERLETSRLLSATCSKEDEILSLQKKLDEINSLRDKIDQDRENIKKQLAEKEDISIYLERENKRIVNQAQE 299
The following BLAST results are available for this feature:
BLAST of PH domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
PLEKHD11.255e-1226.81pleckstrin homology and coiled-coil domain contain... [more]
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BLAST of PH domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
F31D4.52.455e-821.49Pleckstrin homology domain-containing family D mem... [more]
F31D4.52.725e-821.49Pleckstrin homology domain-containing family D mem... [more]
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BLAST of PH domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PH domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
plekhd13.247e-1327.54pleckstrin homology domain containing, family D (w... [more]
plekhd13.888e-1325.83pleckstrin homology domain containing, family D (w... [more]
plekhd14.791e-1325.83pleckstrin homology domain containing, family D (w... [more]
plekhd15.499e-1327.54pleckstrin homology domain containing, family D (w... [more]
plekhd16.312e-1327.54pleckstrin homology domain containing, family D (w... [more]
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BLAST of PH domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
PLEKHD11.440e-1626.14pleckstrin homology and coiled-coil domain contain... [more]
PLEKHD12.137e-1525.13pleckstrin homology and coiled-coil domain contain... [more]
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BLAST of PH domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Plekhd11.233e-1226.81pleckstrin homology domain containing, family D (w... [more]
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BLAST of PH domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|A6NEE1|PLHD1_HUMAN6.027e-1226.81Pleckstrin homology domain-containing family D mem... [more]
sp|B2RPU2|PLHD1_MOUSE8.627e-1226.81Pleckstrin homology domain-containing family D mem... [more]
sp|B1WBU8|PLHD1_RAT8.936e-1226.81Pleckstrin homology domain-containing family D mem... [more]
sp|Q9ST43|PH1_ARATH5.145e-929.46Pleckstrin homology domain-containing protein 1 OS... [more]
sp|O45420|PLHD1_CAEEL3.466e-721.49Pleckstrin homology domain-containing family D mem... [more]
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BLAST of PH domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267GRL22.454e-2228.17PH domain-containing protein (Fragment) OS=Macrost... [more]
A0A1I8I2605.731e-1636.43PH domain-containing protein OS=Macrostomum lignan... [more]
A0A1I8I4921.266e-1536.43PH domain-containing protein OS=Macrostomum lignan... [more]
A0A3S0ZSH21.291e-1527.78PH domain-containing protein OS=Elysia chlorotica ... [more]
A0A267E9Z71.489e-1536.43PH domain-containing protein (Fragment) OS=Macrost... [more]
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BLAST of PH domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PH domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PH domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PH domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PH domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
plekhd13.821e-1225.00solute carrier family 39 member 9 [Source:NCBI gen... [more]
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BLAST of PH domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000007865.10.000e+099.78SMESG000007865.1[more]
SMESG000007865.10.000e+099.56SMESG000007865.1[more]
SMESG000007865.10.000e+0100.00SMESG000007865.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016107 ID=SMED30016107|Name=PH domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1584bp
TTTAAGATTAATATTTCAATTGTTCGATTTTATACAACCAGTTATTTATT
TAAACTTTAATATTTATTTTCCCAAAAATCTGTTTAAATGTCAATTAATA
TTCATTCTGGAGGTTTTGAACTTGATAAGCACGCAGAAAAAAGTGGATTT
TTGTTAAAGCGATCTGATCAGCAAACTAACAAATGGCAAAATCGATTTTG
TATTTTGAAAGATAATTATTTTTTATGGTACAAAGAGGATGAAGCTATGA
AAATGAAAAAATCGAAGTATTTTAATACGAAACCACAAGGAGTTATTTAT
TTAAACGATTGCATTATACGTGAGATAATTGATAGTAGTCAAAACTGTTG
TTTCATAATTGAAAATGCTGCTTTTCAAAAACCACTTGTCTTTTCCGTTG
ATAATCAGTTGGCAAGGGAGTCGTGGATCGAAAACTTAATATTTAATAGT
TCGATAAATTATCACAACATGCTTGAAACCCAAAAATTCGTTAAAGATTT
AATGCAAACGACTTCAACAACGAAGTATGCAAGAGTCAAACAGCAATTCA
GCGACGTCAAGGATGAAAATCAAGTATTAATTAAAATGAAGGAACAATTA
GAAAAAGAGAATGAGGAAAAAGAGCAATTCATTAAAGATTTACAGTTGGA
ATTTTCGAAATTTAAAGAAGAAATGGATTCTGCGTATTTAGTTTTGAATA
CTCTTGAACAATCAAAGTCAAATTTAGAAAAAAAGAATTCCAAGTTGGAA
AATGATTTAAGTAACTTGGAATGTGAAAGATTAGAAACTTCGAGACTCCT
GAGTGCCACTTGTAGTAAAGAAGATGAAATATTATCGCTTCAGAAGAAAT
TAGATGAAATAAATAGTTTGAGAGATAAAATCGATCAAGACCGAGAAAAT
ATTAAGAAACAATTGGCCGAGAAAGAAGATATTTCAATCTATCTCGAAAG
GGAAAATAAAAGAATTGTAAATCAAGCTCAAGAACTAAATAATCTAATAA
ATAAATTGAAAAATCAGAACATTGATGTAGAAAGAAAACTAGAATTGGAA
AAAGCCGCTCGAATTGAAGCTGAAAGACGCTTAAGGGAAGCTGAGATGTC
ACTTGGACGGTTGCATGATAACATAGTGGAAGGCGGTACAAAATATACAA
ATGAGAAAAAAACAGAAATGACGTCAGATGTTAATAAATTAAAAGATTAT
TTCGAGAGTGTTATTAACTCACAATCAACATCACCTAAAGGAGTAGATGA
GAATCATGCAACTCGAGGGAGGTCTTCGAGTAAATCACGAGTTCTTAATT
CTAGAAAGAATTCCATTGATGTGGACAAGTTTGACAAAAATTCAATAGAT
AATCAAACAAAATTGTCATTGCAAATGCCAAAATCGTACAGGGATTCCAT
TCGTGTACGCGAAGAATCCCAAAAGCTAAGAGAAGAACATAGGAAAAGAA
TCAGTCAAATTCAATGGGCCGATGCAGATTAGATTATATTCCAGATGATT
ATATTCAGTCCAGTGTCAGCTAGTGTTTCTAGAGTGCTAATCAATATTTT
GTAAAATAAATATTAATTAATTTTATGAAAAAAA
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protein sequence of SMED30016107-orf-1

>SMED30016107-orf-1 ID=SMED30016107-orf-1|Name=SMED30016107-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=465bp
MSINIHSGGFELDKHAEKSGFLLKRSDQQTNKWQNRFCILKDNYFLWYKE
DEAMKMKKSKYFNTKPQGVIYLNDCIIREIIDSSQNCCFIIENAAFQKPL
VFSVDNQLARESWIENLIFNSSINYHNMLETQKFVKDLMQTTSTTKYARV
KQQFSDVKDENQVLIKMKEQLEKENEEKEQFIKDLQLEFSKFKEEMDSAY
LVLNTLEQSKSNLEKKNSKLENDLSNLECERLETSRLLSATCSKEDEILS
LQKKLDEINSLRDKIDQDRENIKKQLAEKEDISIYLERENKRIVNQAQEL
NNLINKLKNQNIDVERKLELEKAARIEAERRLREAEMSLGRLHDNIVEGG
TKYTNEKKTEMTSDVNKLKDYFESVINSQSTSPKGVDENHATRGRSSSKS
RVLNSRKNSIDVDKFDKNSIDNQTKLSLQMPKSYRDSIRVREESQKLREE
HRKRISQIQWADAD*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000070intestinal phagocyte
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR001849PH_domain
IPR011993PH-like_dom_sf
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 286..338
NoneNo IPR availableCOILSCoilCoilcoord: 154..237
NoneNo IPR availableCOILSCoilCoilcoord: 251..282
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 435..464
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 406..420
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 378..464
NoneNo IPR availablePANTHERPTHR14383SWAP-70 RECOMBINASEcoord: 17..454
NoneNo IPR availablePANTHERPTHR14383:SF1PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY D MEMBER 1coord: 17..454
NoneNo IPR availableSUPERFAMILYSSF50729PH domain-likecoord: 13..118
IPR001849Pleckstrin homology domainSMARTSM00233PH_updatecoord: 16..124
e-value: 3.2E-16
score: 69.9
IPR001849Pleckstrin homology domainPFAMPF00169PHcoord: 17..119
e-value: 2.8E-16
score: 59.9
IPR001849Pleckstrin homology domainPROSITEPS50003PH_DOMAINcoord: 15..122
score: 14.981
IPR011993PH-like domain superfamilyGENE3DG3DSA:2.30.29.30coord: 9..118
e-value: 1.3E-17
score: 65.8