Cell wall integrity and stress response component 1

Overview
NameCell wall integrity and stress response component 1
Smed IDSMED30016089
Length (bp)903
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Cell wall integrity and stress response component 1 (SMED30016089) t-SNE clustered cells

Violin plots show distribution of expression levels for Cell wall integrity and stress response component 1 (SMED30016089) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Cell wall integrity and stress response component 1 (SMED30016089) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Cell wall integrity and stress response component 1 (SMED30016089) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30016089

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30016089h1SMcG0010322 Contig3696GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30016089h1SMcG0020071 Contig3696GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30016089h1SMcG0020071 dd_Smed_v4_9472_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016089h1SMcG0020071 dd_Smed_v4_9472_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Match: A0A5P8I4L8 (Uncharacterized protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 316.62 bits (810), Expect = 3.350e-105
Identity = 149/262 (56.87%), Postives = 193/262 (73.66%), Query Frame = 3 
Query:   93 MCKKNHELSVIGAGQMRTGTKSLKMALEKLYNGKCYHMAEIFENHRNHISLWISVFDKNGLDESTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTRNSDDWLKSVRNTTLKKGSDVTFLSKIFFMFSGLGRFPEFSEKLINHAYKNSYETLINDDAILKQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNLPIPDEPYPFVNSTEEFNKYINKTYLADFVKYGIFVTIFAGAISLIIKTKF*FKHFYCKIF 878
            M  + +E+ VIGAGQMRTGT SLK ALEKLYN  CYHMAEI  +H++H+ LWISVFD   ++E TA  + SDY ATVDFPS AVYKDLM++YPNAKV+LTTRNSDDW+KSV++TTL+     +F+ KI F F+    FP   +K+   AYK  ++ L  DD +LKQCYE+FN+EVR+ VPKERLLEFN+K+GWEPLC FLNLP+P+EPYPFVN+T++F + +    L   ++ G+ +++  G +SLII      KH Y K F
Sbjct:    1 MNHRKNEILVIGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVPNEPYPFVNTTKDFQRNVRGMMLKSVIRSGLVLSLTIGCLSLII------KHIYDKYF 256
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Match: A0A1I8I195 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 4.985e-54
Identity = 91/235 (38.72%), Postives = 140/235 (59.57%), Query Frame = 3 
Query:   93 MCKKNHELSVIGAGQMRTGTKSLKMALEKLYNGKCYHMAEIFENH--RNHISLWISVFDKNGLDESTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTRNSDDWLKSVRNTTLKKGSDVTFLSKIFFMFSGLGRFPEFSEKLINHAYKNSYETLINDDAILKQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNLPIPDEPYPFVNSTEEFNKYIN----KTYLAD 779
            M +K H L VIGAG  RTGT S K+ALE L    CYHM E    H  R HI +WI   + +GL +S A+ +L +Y A VD+P+ + YK+LM+ YPNAKVIL+ R++  W++S++++ LK    +    +      G+G+  +  + +    + ++Y   + D   + + +E+ N  +R  VP ERLLEF    GW PLC FLNLP+PD+P+P +N  ++  + +N     TY++D
Sbjct:    1 MPQKTHSLVVIGAGAPRTGTVSQKVALELLLGQPCYHMGEALGPHNSRRHIPMWIQAVE-SGLSKSLADEILGNYAACVDYPTASFYKELMQHYPNAKVILSVRDTSSWVESMQSSILKAMHTMERFPRPLKWLLGIGQADKMIDAVFRREFGDNYN--LADSEFMARRFEERNDSIRRVVPSERLLEFRVSDGWAPLCQFLNLPVPDKPFPRLNDRKQVERALNIMTAITYVSD 232
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Match: A0A1I8HRJ1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 5.376e-54
Identity = 91/235 (38.72%), Postives = 141/235 (60.00%), Query Frame = 3 
Query:   93 MCKKNHELSVIGAGQMRTGTKSLKMALEKLYNGKCYHMAEIF--ENHRNHISLWISVFDKNGLDESTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTRNSDDWLKSVRNTTLKKGSDVTFLSKIFFMFSGLGRFPEFSEKLINHAYKNSYETLINDDAILKQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNLPIPDEPYPFVNSTEEFNKYIN----KTYLAD 779
            M +K H L VIGAG  RTGT S K+ALE L    CYHM E     N R HI +WI   + +GL +S A+ +L +Y A VD+P+ + YK+LM+ YPNAKVIL+ R+++ W++S++++ LK    +    +      G+G+  +  + +    + ++Y   + D   + + +E+ N  +R  VP ERLLEF    GW PLC FLNLP+PD+P+P +N  ++  + +N     TY++D
Sbjct:    1 MPQKTHSLVVIGAGAPRTGTVSQKVALELLLGQPCYHMGEALGLHNSRRHIPMWIQAVE-SGLSKSLADEILGNYSACVDYPTASFYKELMQHYPNAKVILSVRDTNSWVESMQSSILKAMHTMERFPRPLKWLLGIGQADKMIDAVFRREFGDNYN--LADSEFMARQFEERNDSIRRVVPSERLLEFRVSDGWAPLCQFLNLPVPDKPFPRLNDRKQVERALNIMTAITYVSD 232
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Match: A0A267EUL9 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig019766g2 PE=4 SV=1)

HSP 1 Score: 185.652 bits (470), Expect = 8.869e-54
Identity = 91/235 (38.72%), Postives = 140/235 (59.57%), Query Frame = 3 
Query:   93 MCKKNHELSVIGAGQMRTGTKSLKMALEKLYNGKCYHMAEIFENH--RNHISLWISVFDKNGLDESTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTRNSDDWLKSVRNTTLKKGSDVTFLSKIFFMFSGLGRFPEFSEKLINHAYKNSYETLINDDAILKQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNLPIPDEPYPFVNSTEEFNKYIN----KTYLAD 779
            M +K H L VIGAG  RTGT S K+ALE L    CYHM E    H  R HI +WI   + +GL +S A+ +L +Y A VD+P+ + YK+LM+ YPNAKVIL+ R++  W++S++++ LK    +    +      G+G+  +  + +    + ++Y   + D   + + +E+ N  +R  VP ERLLEF    GW PLC FLNLP+PD+P+P +N  ++  + +N     TY++D
Sbjct:   11 MPQKTHSLVVIGAGAPRTGTVSQKVALELLLGQPCYHMGEALGPHNSRRHIPMWIQAVE-SGLSKSLADEILGNYAACVDYPTASFYKELMQHYPNAKVILSVRDTSSWVESMQSSILKAMHTMERFPRPLKWLLGIGQADKMIDAVFRREFGDNYN--LADSEFMARRFEERNDSIRRVVPSERLLEFRVSDGWAPLCQFLNLPVPDKPFPRLNDRKQVERALNIMTAITYVSD 242
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Match: A0A267EKC2 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig019766g3 PE=4 SV=1)

HSP 1 Score: 185.267 bits (469), Expect = 1.121e-53
Identity = 91/235 (38.72%), Postives = 141/235 (60.00%), Query Frame = 3 
Query:   93 MCKKNHELSVIGAGQMRTGTKSLKMALEKLYNGKCYHMAEIF--ENHRNHISLWISVFDKNGLDESTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTRNSDDWLKSVRNTTLKKGSDVTFLSKIFFMFSGLGRFPEFSEKLINHAYKNSYETLINDDAILKQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNLPIPDEPYPFVNSTEEFNKYIN----KTYLAD 779
            M +K H L VIGAG  RTGT S K+ALE L    CYHM E     N R HI +WI   + +GL +S A+ +L +Y A VD+P+ + YK+LM+ YPNAKVIL+ R+++ W++S++++ LK    +    +      G+G+  +  + +    + ++Y   + D   + + +E+ N  +R  VP ERLLEF    GW PLC FLNLP+PD+P+P +N  ++  + +N     TY++D
Sbjct:   12 MPQKTHSLVVIGAGAPRTGTVSQKVALELLLGQPCYHMGEALGLHNSRRHIPMWIQAVE-SGLSKSLADEILGNYSACVDYPTASFYKELMQHYPNAKVILSVRDTNSWVESMQSSILKAMHTMERFPRPLKWLLGIGQADKMIDAVFRREFGDNYN--LADSEFMARQFEERNDSIRRVVPSERLLEFRVSDGWAPLCQFLNLPVPDKPFPRLNDRKQVERALNIMTAITYVSD 243
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Match: EDO39565 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9S8])

HSP 1 Score: 96.2857 bits (238), Expect = 1.189e-23
Identity = 75/211 (35.55%), Postives = 105/211 (49.76%), Query Frame = 3 
Query:  114 LSVIGAGQMRTGTKSLKMALEKL-YNGKCYHMAEIFENHRNHISLWISVFDKNGLDESTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTRNSDDWLKSVRNTTLKKGSDVTFLSKIFFMFSGLGRFPEFSEKLINHAYKNSYETLINDDAILKQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNLPIPDEPYPF--VNST 737
            + +IGAG  +TGTKSL  AL +L +N   +H     E  + H   +I+ F          E +  +  AT D P+   YK++ K +P+AKVIL+ R+ ++   S R + LK  S V+  +    M  GL   P    KL    Y N  + + N+       Y   N EV  T+P ++LL FN K GWE LC FL   +P  PYP   VNST
Sbjct:    1 MKIIGAGLPKTGTKSLASALRRLGFNVHDHH-----EQLQFHCDEYIAAFKG---PMPNFEEMYENVDATTDLPACIFYKEIFKAFPDAKVILSVRDDEE---SWRKSFLKTESIVSSATSNLLMILGLFLTPS-GRKLWRLGY-NVKKYVKNELD-----YTKHNNEVMATIPAKQLLVFNPKDGWESLCAFLGAEVPAIPYPRENVNST 193
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Match: EDO38503 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCW7])

HSP 1 Score: 93.9745 bits (232), Expect = 6.621e-23
Identity = 75/228 (32.89%), Postives = 106/228 (46.49%), Query Frame = 3 
Query:  114 LSVIGAGQMRTGTKSLKMALEKLYNGKCYHMAEIFENHRNHISLWISVFDKNGLDESTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTR-NSDDWLKSVRNTTLKKGSDVTF------LSKIFFMFSGLGRFPEFSEKLINHAYKNSYETLI-NDDAILKQC-YEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNLPIPDEPYPFVNSTEEF-NKYINKT 767
            +  I AG  +TGTKSL  AL  L     Y + +I EN R H+  WI  F     D +   ++ +     +D P     +D++   P  KVILT R N+++WL     T+L +  D+ +        K+  + +  GR                + TLI +  A LK   Y    +EV+  VP +RLL FN K+GWEPLC FL    P  P+P  N   EF    INK+
Sbjct:    1 MRFICAGLSKTGTKSLVAALRYL----GYTVYDIEENWRYHMDEWIKAFQGTNPDFT---AMYASVDTAIDLPPYLFVRDILVALPELKVILTVRENAEEWL-----TSLTRSLDIWWSVYNQRWLKLARVLTPTGR---------------KWATLIRHQTAALKTLDYTRHIEEVKAIVPADRLLVFNVKEGWEPLCAFLGHEAPSIPFPHRNKKSEFIPDMINKS 201
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Match: EDO49231 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGU4])

HSP 1 Score: 93.2041 bits (230), Expect = 1.844e-22
Identity = 71/247 (28.74%), Postives = 117/247 (47.37%), Query Frame = 3 
Query:  114 LSVIGAGQMRTGTKSLKMALEKLYNGKCYHMAEIFENHRNHISLWISVFDKNGLDESTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTRNSDDWLKSVR-NTTLKKGSDVTFLSKIFFMFSGLGRFPEFSEKLINHAYKNSYETLINDDA--ILKQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNLPIPDEPYPFVNSTEEFNKYINKTYLAD-------FVKYGIFVTIFAGAI 824
            + VI AG  +T TKS+  AL  L     Y++ ++ E        W+ + +   L +   E++  +  A  D P    +++++K  P+AKVILT R  + WL+S +    + +G      SK++F+   L        K+ N      Y T IN ++  I+ +  E  NQ VR TVP  +LLE++  +GW PLC FL    P E +P  N   EF K        +        ++ G+ +++ AG +
Sbjct:    1 MKVICAGICKTATKSMAKALRIL----GYNVYDVEEQICELFDDWLDLIEHGRLPDF--EAMFENVDAITDLPGNFFFEEILKASPDAKVILTVREQEAWLRSSKKQLKMVQG----LFSKLWFL--SLSPIYLGIRKITNGMVAALYGT-INAESTWIIAKALEMHNQRVRSTVPPGQLLEYSVTEGWGPLCDFLGHATPKEKFPHENVKGEFTKRWEGVPSGEKKLGRRILLEIGVRLSVIAGGL 234
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Match: EDO47446 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RM57])

HSP 1 Score: 91.6633 bits (226), Expect = 2.304e-22
Identity = 60/213 (28.17%), Postives = 108/213 (50.70%), Query Frame = 3 
Query:  114 LSVIGAGQMRTGTKSLKMALEKLYNGKCYHMAEIFENHRNHISLWISVFDKNGLDESTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTRNSDD-WLKSV-RNTTLKKGSDVTFLSKIFFMFSGLGRFPEFSEKLINHAYKNSYETLINDDAILKQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNLPIPDEPYPFVNSTEEF 746
            + ++GAG  +TGTKSL  AL  L     Y + +  +  ++H+  +I+ F+ N      ++ +     AT   P+   YK++   +PNA+VIL+ R++++ W KS+ ++  + K S  +    +    S  GR  +  +  I++         + +       Y+  N +V  ++   +LL FN K+GWEPLC FL + +P  P+P VN   E+
Sbjct:    1 MKIVGAGLPKTGTKSLAAALRHL----GYKVHDGPQQWQHHLDEYIAAFNGN---IPNSKEMYECVDATTASPACFFYKEISAAFPNARVILSVRDNEEIWRKSLDKSLQVSKASSRSLWMMVGKQISPTGRKWKTIQDFISNTL------FVKNTDKDSNIYQKHNDDVIASISANQLLVFNPKEGWEPLCAFLGVEVPAIPFPRVNVDSEY 200
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Match: EDO41210 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4T7])

HSP 1 Score: 92.8189 bits (229), Expect = 3.824e-22
Identity = 69/235 (29.36%), Postives = 119/235 (50.64%), Query Frame = 3 
Query:  114 LSVIGAGQMRTGTKSLKMALE----KLYNGK---CYHMAEIFENHRNHISLWISVFDKNGLDESTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTR-NSDDWLKSV--RNTTLKKGSDVTFLSKIFFMFSGLG-RFPEFSEKLINHAYKNSYETLINDDAIL--KQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNLPIPDEPYPFVN-STEEFNKYINKTYLA 776
            + VI AG  +TGTKSL  AL     K+Y+      YHM E           WI  F+ + + +     +  D  A  D P    Y++L   +P AKVIL+ R N+D W +S+      LK+   +  +  + F+ +  G +       ++N A+K++     N +A +  K+ Y + N+ V+  +PK++LL F  +QGW PLC FL +  P+  +P+ N ++E+   +++ + +A
Sbjct:   23 MKVICAGMPKTGTKSLAKALRILGLKVYDADEHCIYHMEE-----------WIQAFETSKMPDLY--RMYKDVDAVTDVPPMFWYEELHYAFPEAKVILSVRDNTDVWYESLLWNQKVLKEAMSLRVM--LGFLLTPTGWKMSRMMSDMMNAAFKST-----NPNAAILNKKAYREHNERVKNVIPKDKLLVFKVEQGWPPLCRFLEIAEPEIFFPYENVNSEDVKAFLHDSKIA 237
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Match: SMESG000035040.1 (SMESG000035040.1)

HSP 1 Score: 518.464 bits (1334), Expect = 0.000e+0
Identity = 250/252 (99.21%), Postives = 251/252 (99.60%), Query Frame = 3 
Query:   93 MCKKNHELSVIGAGQMRTGTKSLKMALEKLYNGKCYHMAEIFENHRNHISLWISVFDKNGLDESTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTRNSDDWLKSVRNTTLKKGSDVTFLSKIFFMFSGLGRFPEFSEKLINHAYKNSYETLINDDAILKQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNLPIPDEPYPFVNSTEEFNKYINKTYLADFVKYGIFVTIFAGAISLIIKTKF 848
            M KKNHELSVIGAGQMRTGTKSLKMALEKLYNGKCYHMAEIFENHRNHISLWISVFDKNGLD+STAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTRNSDDWLKSVRNTTLKKGSDVTFLSKIFFMFSGLGRFPEFSEKLINHAYKNSYETLINDDAILKQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNLPIPDEPYPFVNSTEEFNKYINKTYLADFVKYGIFVTIFAGAISLIIKTKF
Sbjct:    1 MRKKNHELSVIGAGQMRTGTKSLKMALEKLYNGKCYHMAEIFENHRNHISLWISVFDKNGLDKSTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTRNSDDWLKSVRNTTLKKGSDVTFLSKIFFMFSGLGRFPEFSEKLINHAYKNSYETLINDDAILKQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNLPIPDEPYPFVNSTEEFNKYINKTYLADFVKYGIFVTIFAGAISLIIKTKF 252
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Match: SMESG000035036.1 (SMESG000035036.1)

HSP 1 Score: 315.464 bits (807), Expect = 2.208e-108
Identity = 150/262 (57.25%), Postives = 192/262 (73.28%), Query Frame = 3 
Query:   93 MCKKNHELSVIGAGQMRTGTKSLKMALEKLYNGKCYHMAEIFENHRNHISLWISVFDKNGLDESTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTRNSDDWLKSVRNTTLKKGSDVTFLSKIFFMFSGLGRFPEFSEKLINHAYKNSYETLINDDAILKQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNLPIPDEPYPFVNSTEEFNKYINKTYLADFVKYGIFVTIFAGAISLIIKTKF*FKHFYCKIF 878
            M  + +EL VIGAGQMRTGT SLK ALEKLYN  CYHMAEI  +H++H+ LWISVFD   ++E TA  + SDY ATVDFPS AVYKDLM++YPNAKV+LTTRNSDDW+KSV++TTL+     +F+ KI F F+    FP   +K+   AYK  ++ L  DD +LKQCYEDFN+EVR+ VP+ERLLEFN+K+GWEPLC FLNLP+P+EPYPFVN+T++F   I    L   ++ G+ +++  G +S+II      KH Y K F
Sbjct:    1 MNHRKNELLVIGAGQMRTGTFSLKFALEKLYNCPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEDFNEEVRKYVPRERLLEFNAKEGWEPLCRFLNLPVPNEPYPFVNTTKDFQLTIRGMMLKSVIRSGLVLSLTIGCLSIII------KHIYDKYF 256
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Match: SMESG000035037.1 (SMESG000035037.1)

HSP 1 Score: 314.694 bits (805), Expect = 4.744e-108
Identity = 149/262 (56.87%), Postives = 192/262 (73.28%), Query Frame = 3 
Query:   93 MCKKNHELSVIGAGQMRTGTKSLKMALEKLYNGKCYHMAEIFENHRNHISLWISVFDKNGLDESTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTRNSDDWLKSVRNTTLKKGSDVTFLSKIFFMFSGLGRFPEFSEKLINHAYKNSYETLINDDAILKQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNLPIPDEPYPFVNSTEEFNKYINKTYLADFVKYGIFVTIFAGAISLIIKTKF*FKHFYCKIF 878
            M  + +E+ VIGAGQMRTGT SLK ALEKLYN  CYHMAEI  +H++H+ LWISVFD   ++E TA  + SDY ATVDFPS AVYKDLM++YPNAKV+LTTRNSDDW+KSV++TTL+     +F+ KI F F+    FP   +K+   AYK  ++ L  DD +LKQCYEDFN+EVR+ VPKERLLEFN+K+GWEPLC FLNL +P+EPYPFVN+T++F + +    L   ++ G+ +++  G +SLII      KH Y K F
Sbjct:    1 MNHRKNEILVIGAGQMRTGTFSLKFALEKLYNCPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMFLKAYKEPFDALRTDDELLKQCYEDFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLQVPNEPYPFVNTTKDFQRNVRGMMLKSVIRSGLVLSLTIGCLSLII------KHIYDKYF 256
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Match: SMESG000035096.1 (SMESG000035096.1)

HSP 1 Score: 314.309 bits (804), Expect = 7.588e-108
Identity = 149/262 (56.87%), Postives = 188/262 (71.76%), Query Frame = 3 
Query:   93 MCKKNHELSVIGAGQMRTGTKSLKMALEKLYNGKCYHMAEIFENHRNHISLWISVFDKNGLDESTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTRNSDDWLKSVRNTTLKKGSDVTFLSKIFFMFSGLGRFPEFSEKLINHAYKNSYETLINDDAILKQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNLPIPDEPYPFVNSTEEFNKYINKTYLADFVKYGIFVTIFAGAISLIIKTKF*FKHFYCKIF 878
            M  + +E+ VIGAGQMRTGT SLK ALEKLYN  CYHMAEI  +H++H+ LWISVFD   ++E TA  + SDY ATVDFPS AVYKDLM++YPNAKV+LTTRNSDDW+KSVR+TTLK     +   K    FS    FP   +K+   AYK  ++ +  DD +LKQCYEDFN+EVR  VPKERLLEFN+K+GWEPLC FLNLP+P+EPYPFVN+T++F + +    L   ++ G+ +++  G +SLII      KH Y K F
Sbjct:    1 MNHRKNEILVIGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVRSTTLKDEMRTSLFDKFLLFFSIGTEFPVMCDKMFLKAYKKPFDAVRADDELLKQCYEDFNEEVRIYVPKERLLEFNAKEGWEPLCRFLNLPVPNEPYPFVNTTQDFQRNVRGIMLKSLIRSGLAISLTIGCLSLII------KHIYDKYF 256
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BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Match: SMESG000035035.1 (SMESG000035035.1)

HSP 1 Score: 270.396 bits (690), Expect = 8.320e-91
Identity = 129/245 (52.65%), Postives = 165/245 (67.35%), Query Frame = 3 
Query:  114 LSVIGAGQMRTGTKSLKMALEKLYNGKCYHMAEIFENHRNHISLWISVFDKNGLDESTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTRNSDDWLKSVRNTTLKKGSDVTFLSKIFFMFSGLGRFPEFSEKLINHAYKNSYETLINDDAILKQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNLPIPDEPYPFVNSTEEFNKYINKTYLADFVKYGIFVTIFAGAISLIIKTKF 848
            + VIGAGQMRTGT SLK+ALEKLYNGKCY++ E+F+ H++H  +W SV +   L +  AES+LS+YCA  D P + VYKDLM++YP+AKVILT RNSDDW+KSVR TT+          K  F   G   F E   K I+  YK  +E L+ +D +LK+ YE++N EVR  VP ERLLEFN  +GWEPLC FLNLP P+EP+PFVNSTEEF  Y NK     F + G F   F  ++ ++  + F
Sbjct:    1 MYVIGAGQMRTGTNSLKIALEKLYNGKCYNIKEVFDYHQHHFEVWNSVLETEELRKDVAESILSEYCAVTDIPCSIVYKDLMEIYPDAKVILTIRNSDDWIKSVRMTTMTSEVHKMMRWKFLFWIFGSWTFMELVAKSISRIYKQPFELLMTNDVVLKKAYEEYNNEVRTFVPPERLLEFNVNEGWEPLCKFLNLPFPNEPFPFVNSTEEFLNYFNKKIKKYFARIGCFSIFFGFSVGIVATSYF 245
The following BLAST results are available for this feature:
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5P8I4L83.350e-10556.87Uncharacterized protein OS=Schmidtea mediterranea ... [more]
A0A1I8I1954.985e-5438.72Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8HRJ15.376e-5438.72Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267EUL98.869e-5438.72Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A267EKC21.121e-5338.72Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO395651.189e-2335.55Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO385036.621e-2332.89Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO492311.844e-2228.74Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO474462.304e-2228.17Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO412103.824e-2229.36Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000035040.10.000e+099.21SMESG000035040.1[more]
SMESG000035036.12.208e-10857.25SMESG000035036.1[more]
SMESG000035037.14.744e-10856.87SMESG000035037.1[more]
SMESG000035096.17.588e-10856.87SMESG000035096.1[more]
SMESG000035035.18.320e-9152.65SMESG000035035.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016089 ID=SMED30016089|Name=Cell wall integrity and stress response component 1|organism=Schmidtea mediterranea sexual|type=transcript|length=903bp
TCTAATTATTCTAATACTTCAATTGTGCGTATTTTGTGGTGTTAGTGAAC
TTTAACATATATGGAATGACGTAGTTGTATCATTTATACACAATGTGTAA
AAAAAATCATGAGCTCTCCGTAATTGGAGCCGGCCAAATGAGAACTGGGA
CGAAGTCGTTGAAAATGGCGCTAGAGAAACTATACAATGGGAAGTGTTAT
CATATGGCGGAAATATTTGAGAATCACCGTAATCATATCAGTTTATGGAT
ATCAGTGTTTGACAAAAATGGCCTAGACGAAAGCACCGCTGAAAGCTTAC
TATCAGACTATTGTGCGACTGTTGATTTTCCGTCGACTGCCGTTTACAAA
GATTTAATGAAACTCTATCCTAATGCCAAAGTGATATTAACAACGAGAAA
TTCAGATGACTGGCTAAAGAGTGTAAGAAACACGACACTGAAAAAAGGCT
CCGATGTCACTTTTTTGAGTAAAATATTTTTTATGTTCAGTGGATTGGGG
AGATTTCCTGAATTTTCTGAAAAATTAATTAATCATGCGTATAAAAATTC
GTATGAAACCCTCATAAATGATGATGCAATTCTCAAGCAATGCTATGAAG
ACTTTAATCAAGAGGTCAGAGAGACTGTTCCCAAGGAAAGATTATTGGAA
TTCAACTCGAAACAAGGATGGGAGCCTTTATGTACATTCCTAAATTTACC
CATTCCGGACGAACCGTATCCATTTGTCAATTCGACTGAAGAATTCAACA
AATATATAAACAAAACATATTTAGCTGATTTTGTGAAATATGGAATTTTT
GTTACGATTTTTGCTGGTGCGATTAGTTTGATCATCAAAACAAAATTCTA
ATTTAAACATTTTTATTGCAAAATTTTTTACAAAAATAAATTTATTATAA
TTT
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protein sequence of SMED30016089-orf-1

>SMED30016089-orf-1 ID=SMED30016089-orf-1|Name=SMED30016089-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=253bp
MCKKNHELSVIGAGQMRTGTKSLKMALEKLYNGKCYHMAEIFENHRNHIS
LWISVFDKNGLDESTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILT
TRNSDDWLKSVRNTTLKKGSDVTFLSKIFFMFSGLGRFPEFSEKLINHAY
KNSYETLINDDAILKQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFL
NLPIPDEPYPFVNSTEEFNKYINKTYLADFVKYGIFVTIFAGAISLIIKT
KF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
Vocabulary: INTERPRO
TermDefinition
IPR040632Sulfotransfer_4
IPR027417P-loop_NTPase
IPR000159RA_dom
Vocabulary: biological process
TermDefinition
GO:0007165signal transduction
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR040632Sulfotransferase, S. mansonii-typePFAMPF17784Sulfotransfer_4coord: 10..218
e-value: 2.3E-63
score: 213.9
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 1..233
e-value: 6.9E-70
score: 237.5
NoneNo IPR availablePANTHERPTHR36978FAMILY NOT NAMEDcoord: 4..226
NoneNo IPR availableTMHMMTMhelixcoord: 230..248
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 10..220