SMED30016072
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30016072 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Alignments
SMED30016072 aligns in the following genomic locations:
Homology
BLAST of SMED30016072 vs. TrEMBL
Match: A0A267H992 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig005923g1 PE=4 SV=1) HSP 1 Score: 77.7962 bits (190), Expect = 7.957e-13 Identity = 51/141 (36.17%), Postives = 81/141 (57.45%), Query Frame = 1 Query: 394 IQQESHDIQLHRKIRQPWYGVGKYPFRIAVAXXXXXXXXXXXXXXXXXXXSFGNIFLSMDTGQILGPSFVVLAIICFAIGIKSHYKSYLISEKSRSKLKFRGVDGKMVSMTT------KLPESNTRDTLVSGTQSEMAVTK 798 I++E D +L R ++ P YG G+ PF IAVA ++L ++ G+ +++L NI LS+D G+I+GP + L I A GIK Y +Y+IS R+++KF ++ TT K P + + L+SGT S AV++ Sbjct: 108 IRREYQDKKLRRNLQSPMYGKGRTPFFIAVALFVLGGVLILLGVVVLLLRFHNNIMLSIDKGEIIGPLLISLGIASLAGGIKFLYDAYIISNNERNRIKFTSASDSLIQATTNRAGPVKAPPT---EMLMSGTVSANAVSR 245
BLAST of SMED30016072 vs. TrEMBL
Match: A0A267EJ81 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig032634g1 PE=4 SV=1) HSP 1 Score: 77.411 bits (189), Expect = 8.359e-13 Identity = 51/141 (36.17%), Postives = 81/141 (57.45%), Query Frame = 1 Query: 394 IQQESHDIQLHRKIRQPWYGVGKYPFRIAVAXXXXXXXXXXXXXXXXXXXSFGNIFLSMDTGQILGPSFVVLAIICFAIGIKSHYKSYLISEKSRSKLKFRGVDGKMVSMTT------KLPESNTRDTLVSGTQSEMAVTK 798 I++E D +L R ++ P YG G+ PF IAVA ++L ++ G+ +++L NI LS+D G+I+GP + L I A GIK Y +Y+IS R+++KF ++ TT K P + + L+SGT S AV++ Sbjct: 108 IRREYQDKKLRRNLQSPMYGKGRTPFFIAVALFVLGSVLILLGVVVLLLRFHNNIMLSIDKGEIIGPLLISLGIASLAGGIKFLYDAYIISNNERNRIKFTSASDSLIQATTNRAGPVKAPPT---EMLMSGTVSANAVSR 245
BLAST of SMED30016072 vs. TrEMBL
Match: A0A267E0J3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig007171g1 PE=4 SV=1) HSP 1 Score: 77.411 bits (189), Expect = 9.786e-13 Identity = 51/141 (36.17%), Postives = 81/141 (57.45%), Query Frame = 1 Query: 394 IQQESHDIQLHRKIRQPWYGVGKYPFRIAVAXXXXXXXXXXXXXXXXXXXSFGNIFLSMDTGQILGPSFVVLAIICFAIGIKSHYKSYLISEKSRSKLKFRGVDGKMVSMTT------KLPESNTRDTLVSGTQSEMAVTK 798 I++E D +L R ++ P YG G+ PF IAVA ++L ++ G+ +++L NI LS+D G+I+GP + L I A GIK Y +Y+IS R+++KF ++ TT K P + + L+SGT S AV++ Sbjct: 108 IRREYQDKKLRRNLQSPMYGKGRTPFFIAVALFVLGSVLILLGVVVLLLRFHNNIMLSIDKGEIIGPLLISLGIASLAGGIKFLYDAYVISNNERNRIKFTSASDSLIQATTNRAGPVKAPPT---EMLMSGTVSANAVSR 245
BLAST of SMED30016072 vs. TrEMBL
Match: A0A267GI24 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig015535g1 PE=4 SV=1) HSP 1 Score: 75.0998 bits (183), Expect = 7.051e-12 Identity = 50/140 (35.71%), Postives = 81/140 (57.86%), Query Frame = 1 Query: 394 IQQESHDIQLHRKIRQPWYGVGKYPFRIAVAXXXXXXXXXXXXXXXXXXXSFGNIFLSMDTGQILGPSFVVLAIICFAIGIKSHYKSYLISEKSRSKLKFRGVDGKMVSMTT-----KLPESNTRDTLVSGTQSEMAVTK 798 I+QE HD +L + P YG G+ PFRI++A +++ ++ G+TL++L NI LS+D G+I+GP + + + A G+K Y +Y IS R K+KF D ++S T K ++ + LVSGT + AV++ Sbjct: 107 IRQEYHDKKLRTNFQSPNYGRGRTPFRISIALFVVGGIIIMVGVTLLLLRFHNNIMLSVDKGEIIGPILIAIGVGAIAGGVKFCYDAYKISNSERQKIKFTAADQSLISARTMDSARKFKQA-PGEVLVSGTVTANAVSR 245
BLAST of SMED30016072 vs. TrEMBL
Match: A0A267G8F9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig015535g3 PE=4 SV=1) HSP 1 Score: 75.0998 bits (183), Expect = 7.120e-12 Identity = 50/140 (35.71%), Postives = 81/140 (57.86%), Query Frame = 1 Query: 394 IQQESHDIQLHRKIRQPWYGVGKYPFRIAVAXXXXXXXXXXXXXXXXXXXSFGNIFLSMDTGQILGPSFVVLAIICFAIGIKSHYKSYLISEKSRSKLKFRGVDGKMVSMTT-----KLPESNTRDTLVSGTQSEMAVTK 798 I+QE HD +L + P YG G+ PFRI++A +++ ++ G+TL++L NI LS+D G+I+GP + + + A G+K Y +Y IS R K+KF D ++S T K ++ + LVSGT + AV++ Sbjct: 107 IRQEYHDKKLRTNFQSPNYGRGRTPFRISIALFVVGGIIIMVGVTLLLLRFHNNIMLSVDKGEIIGPILIAIGVGAIAGGVKFCYDAYKISNSERQKIKFTAADQSLISARTMDSARKFKQA-PGEVLVSGTVTANAVSR 245
BLAST of SMED30016072 vs. Planmine SMEST
Match: SMESG000064318.1 (SMESG000064318.1) HSP 1 Score: 396.741 bits (1018), Expect = 9.303e-141 Identity = 222/254 (87.40%), Postives = 225/254 (88.58%), Query Frame = 1 Query: 58 MSEFSHKNKLKQLKKDHSTSSSHNYQNDFHGNQSILSYNSTRSSNDILSSQPSFLSGEISIKSKDRDLATNFTNSAHVIGVTQSVRSLKCNMPXXXXXXXXXXXXXXXXXXXIQQESHDIQLHRKIRQPWYGVGKYPFRIAVAXXXXXXXXXXXXXXXXXXXSFGNIFLSMDTGQILGPSFVVLAIICFAIGIKSHYKSYLISEKSRSKLKFRGVDGKMVSMTTKLPESNTRDTLVSGTQSEMAVTKKYDQFSH 819 MSEFSHKNKLKQLKKDHSTSSSHNYQNDFHGNQSILSYNSTRSSNDILSSQPSFLSGEISIKSKDRDLATNFTNSAHVIG++ + IQQESHDIQLHRKIRQPWYGVGKYPFRIAVAFYILFLTFLITGLTLIVLTSFGNIFLSMDTGQILGPSFVVLAIICFAIGIKSHYKSYLISEKSRSKLKFRGVDGKMVSMTTKLPESNTRDTLVSGTQSEMAVTKKYDQFSH Sbjct: 1 MSEFSHKNKLKQLKKDHSTSSSHNYQNDFHGNQSILSYNSTRSSNDILSSQPSFLSGEISIKSKDRDLATNFTNSAHVIGISYN----------------------------IQQESHDIQLHRKIRQPWYGVGKYPFRIAVAFYILFLTFLITGLTLIVLTSFGNIFLSMDTGQILGPSFVVLAIICFAIGIKSHYKSYLISEKSRSKLKFRGVDGKMVSMTTKLPESNTRDTLVSGTQSEMAVTKKYDQFSH 226
BLAST of SMED30016072 vs. Planmine SMEST
Match: SMESG000035447.1 (SMESG000035447.1) HSP 1 Score: 108.612 bits (270), Expect = 1.612e-27 Identity = 59/136 (43.38%), Postives = 92/136 (67.65%), Query Frame = 1 Query: 394 IQQESHDIQLHRKIRQPWYGVGKYPFRIAVAXXXXXXXXXXXXXXXXXXXSFGNIFLSMDTGQILGPSFVVLAIICFAIGIKSHYKSYLISEKSRSKLKFRGVDGKMVSMTTKLPESNTRDTLVSGTQSEMAVTKK 801 IQQE HDI++ +K+ PW+G + PF +A+ F+ L + FL GLT++ L FGNIFLS+D G+I+GP F+ L+II A+GIK +Y I+E R K+KFRG + M+++T ++ LV+GT ++ A+++K Sbjct: 125 IQQEYHDIKVQKKLTSPWFGAARKPFMLAMIFFSLAVLFLGIGLTILFLRGFGNIFLSLDVGEIIGPIFIALSIIWLALGIKFIIDAYNIAEMERRKIKFRGDNATMINVTKGEITKRPQEKLVTGTNTKEAISRK 260
BLAST of SMED30016072 vs. Planmine SMEST
Match: SMESG000035447.1 (SMESG000035447.1) HSP 1 Score: 108.612 bits (270), Expect = 1.820e-27 Identity = 59/136 (43.38%), Postives = 92/136 (67.65%), Query Frame = 1 Query: 394 IQQESHDIQLHRKIRQPWYGVGKYPFRIAVAXXXXXXXXXXXXXXXXXXXSFGNIFLSMDTGQILGPSFVVLAIICFAIGIKSHYKSYLISEKSRSKLKFRGVDGKMVSMTTKLPESNTRDTLVSGTQSEMAVTKK 801 IQQE HDI++ +K+ PW+G + PF +A+ F+ L + FL GLT++ L FGNIFLS+D G+I+GP F+ L+II A+GIK +Y I+E R K+KFRG + M+++T ++ LV+GT ++ A+++K Sbjct: 131 IQQEYHDIKVQKKLTSPWFGAARKPFMLAMIFFSLAVLFLGIGLTILFLRGFGNIFLSLDVGEIIGPIFIALSIIWLALGIKFIIDAYNIAEMERRKIKFRGDNATMINVTKGEITKRPQEKLVTGTNTKEAISRK 266
BLAST of SMED30016072 vs. Planmine SMEST
Match: SMESG000035447.1 (SMESG000035447.1) HSP 1 Score: 108.612 bits (270), Expect = 2.237e-27 Identity = 59/136 (43.38%), Postives = 92/136 (67.65%), Query Frame = 1 Query: 394 IQQESHDIQLHRKIRQPWYGVGKYPFRIAVAXXXXXXXXXXXXXXXXXXXSFGNIFLSMDTGQILGPSFVVLAIICFAIGIKSHYKSYLISEKSRSKLKFRGVDGKMVSMTTKLPESNTRDTLVSGTQSEMAVTKK 801 IQQE HDI++ +K+ PW+G + PF +A+ F+ L + FL GLT++ L FGNIFLS+D G+I+GP F+ L+II A+GIK +Y I+E R K+KFRG + M+++T ++ LV+GT ++ A+++K Sbjct: 142 IQQEYHDIKVQKKLTSPWFGAARKPFMLAMIFFSLAVLFLGIGLTILFLRGFGNIFLSLDVGEIIGPIFIALSIIWLALGIKFIIDAYNIAEMERRKIKFRGDNATMINVTKGEITKRPQEKLVTGTNTKEAISRK 277
BLAST of SMED30016072 vs. Planmine SMEST
Match: SMESG000078527.1 (SMESG000078527.1) HSP 1 Score: 77.0258 bits (188), Expect = 5.105e-16 Identity = 51/139 (36.69%), Postives = 77/139 (55.40%), Query Frame = 1 Query: 394 IQQESHDIQLHRKIRQPWYGVGKYPFRIAVAXXXXXXXXXXXXXXXXXXXSFGNIFLSMDTGQILGPSFVVLAIICFAIGIKSHYKSYLISEKSRSKLKFRGVDGKMVSMTTKLPESNTRDT----LVSGTQSEMAVTK 798 IQQE HDI++ +KI YG K PFR ++ +++ F + GLT+I+ +G +FLSMD G+I+GP F+V +I ++GIK +Y + R +L FR MV + T P N T + SG+ A+ K Sbjct: 134 IQQEFHDIKVQKKINSLAYGHAKRPFRYSIICFVIGGVFFLIGLTVIMFRLYGQLFLSMDKGEIIGPIFIVSGVILLSVGIKFMVDAYRYGQLERKRLGFRAAGPNMVVLKTT-PNHNVTPTANNYVQSGSAGAQAINK 271 The following BLAST results are available for this feature:
BLAST of SMED30016072 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30016072 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30016072 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30016072 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30016072 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30016072 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30016072 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30016072 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30016072 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30016072 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30016072 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30016072 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30016072 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30016072 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30016072 ID=SMED30016072|Name=SMED30016072|organism=Schmidtea mediterranea sexual|type=transcript|length=934bpback to top protein sequence of SMED30016072-orf-1 >SMED30016072-orf-1 ID=SMED30016072-orf-1|Name=SMED30016072-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=255bp MSEFSHKNKLKQLKKDHSTSSSHNYQNDFHGNQSILSYNSTRSSNDILSSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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