Retrovirus-related Pol polyprotein from transposon

Overview
NameRetrovirus-related Pol polyprotein from transposon
Smed IDSMED30016067
Length (bp)609
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Retrovirus-related Pol polyprotein from transposon (SMED30016067) t-SNE clustered cells

Violin plots show distribution of expression levels for Retrovirus-related Pol polyprotein from transposon (SMED30016067) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30016067

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30016067SMESG000073188.1 SMESG000070137.1 SMESG000003871.1 dd_Smed_v4_22501_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016067SMESG000073188.1 SMESG000070137.1 SMESG000003871.1 dd_Smed_v4_22501_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30016067SMESG000073188.1 SMESG000070137.1 SMESG000003871.1 dd_Smed_v4_22501_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Zebrafish
Match: BX890543.1 (pep chromosome:GRCz11:14:42224893:42231293:-1 gene:ENSDARG00000114583.1 transcript:ENSDART00000185486.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX890543.1)

HSP 1 Score: 75.8702 bits (185), Expect = 7.136e-24
Identity = 33/92 (35.87%), Postives = 55/92 (59.78%), Query Frame = 3 
Query:   42 HLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNVI 317
            H++ V +V +RLLE  L +K  KC +    +  +G I S   +Q +P  +R + ++PTP     +++FLG AN+YR+F +NF  +A PL  +
Sbjct:  679 HIQHVRQVLQRLLENQLYVKAEKCVFHAQSIPFLGFIISAGEIQADPCKIRAVAEWPTPDSRKALQRFLGFANFYRRFIRNFGQIAAPLTAL 770

HSP 2 Score: 52.7582 bits (125), Expect = 7.136e-24
Identity = 29/98 (29.59%), Postives = 48/98 (48.98%), Query Frame = 1 
Query:  334 FKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIGE----------KDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIG 597
            FKW +  QEAFD+L    +   +L  P+ ++ FI+  +AS+  +G           K H  A+ S  L   E+NY    ++ LA+   +  +R +L G
Sbjct:  777 FKWNSDAQEAFDELKSRFVSAPVLSIPDPEQQFIVEVDASDVGVGAVLSQRSCLDGKVHPCAFFSHRLNPSERNYDVGNRELLAVRLALGEWRHWLEG 874
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Zebrafish
Match: CR354395.2 (pep chromosome:GRCz11:12:13553730:13556536:-1 gene:ENSDARG00000101547.2 transcript:ENSDART00000166396.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR354395.2)

HSP 1 Score: 77.411 bits (189), Expect = 8.475e-24
Identity = 30/99 (30.30%), Postives = 65/99 (65.66%), Query Frame = 3 
Query:   27 NNIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNVIKE 323
            N + EH++ V  V +R+++  L  K  KCE+ ++++  +G++ S  G+  +   V+ ++++P P+++ ++++FLG AN+YR+F + FS +A PL  + +
Sbjct:  335 NTMKEHVEHVRMVLQRMIQHRLYAKLEKCEFHQTQIAFLGYVISAEGITMDDTKVQAVQRWPLPQNLKELQRFLGFANFYRRFIRGFSSIAAPLTAMTK 433

HSP 2 Score: 51.2174 bits (121), Expect = 8.475e-24
Identity = 31/106 (29.25%), Postives = 48/106 (45.28%), Query Frame = 1 
Query:  310 MLLKKQTKFKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIG----------EKDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIG 597
            M  +   K  W++  ++AF  L        ILR PN    FI+  +ASN  +G           K +  A+ SR L S E+NY    ++ LA+   +  +R +L G
Sbjct:  431 MTKRNSHKLSWSSEARQAFSDLKTQFTTAPILRHPNPDLPFIVEVDASNTGVGAVLSQRQGQPSKMYPCAFFSRKLTSAERNYDVGNRELLAMKLALEEWRHWLEG 536
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Zebrafish
Match: BX571665.1 (pep chromosome:GRCz11:17:35431724:35437222:1 gene:ENSDARG00000111789.1 transcript:ENSDART00000190293.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX571665.1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.268e-23
Identity = 30/99 (30.30%), Postives = 65/99 (65.66%), Query Frame = 3 
Query:   27 NNIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNVIKE 323
            N + EH++ V  V +R+++  L  K  KCE+ ++++  +G++ S  G+  +   V+ ++++P P+++ ++++FLG AN+YR+F + FS +A PL  + +
Sbjct:  670 NTMKEHVEHVRMVLQRMIQHRLYAKLEKCEFHQTQIAFLGYVISAEGITMDDTKVQAVQRWPLPQNLKELQRFLGFANFYRRFIRGFSSIAAPLTAMTK 768

HSP 2 Score: 51.6026 bits (122), Expect = 1.268e-23
Identity = 31/106 (29.25%), Postives = 48/106 (45.28%), Query Frame = 1 
Query:  310 MLLKKQTKFKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIG----------EKDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIG 597
            M  +   K  W++  ++AF  L        ILR PN    FI+  +ASN  +G           K +  A+ SR L S E+NY    ++ LA+   +  +R +L G
Sbjct:  766 MTKRNSHKLSWSSEARQAFSDLKTQFTTAPILRHPNPDLPFIVEVDASNTGVGAVLSQRQGQPSKMYPCAFFSRKLTSAERNYDVGNRELLAMKLALEEWRHWLEG 871
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Zebrafish
Match: CR533578.3 (pep chromosome:GRCz11:16:23466094:23471387:-1 gene:ENSDARG00000115891.1 transcript:ENSDART00000180899.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR533578.3)

HSP 1 Score: 76.6406 bits (187), Expect = 1.311e-23
Identity = 30/99 (30.30%), Postives = 65/99 (65.66%), Query Frame = 3 
Query:   27 NNIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNVIKE 323
            N + EH++ V  V +R+++  L  K  KCE+ ++++  +G++ S  G+  +   V+ ++++P P+++ ++++FLG AN+YR+F + FS +A PL  + +
Sbjct:  677 NTMKEHVEHVRMVLQRMIQHRLYAKLEKCEFHQTQIAFLGYVISAEGITMDDTKVQAVQRWPLPQNLKELQRFLGFANFYRRFIRGFSSIAAPLTAMTK 775

HSP 2 Score: 51.2174 bits (121), Expect = 1.311e-23
Identity = 31/106 (29.25%), Postives = 48/106 (45.28%), Query Frame = 1 
Query:  310 MLLKKQTKFKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIG----------EKDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIG 597
            M  +   K  W++  ++AF  L        ILR PN    FI+  +ASN  +G           K +  A+ SR L S E+NY    ++ LA+   +  +R +L G
Sbjct:  773 MTKRNSHKLSWSSEARQAFSDLKTQFTTAPILRHPNPDLPFIVEVDASNTGVGAVLSQRQGQPSKMYPCAFFSRKLTSAERNYDVGNRELLAMKLALEEWRHWLEG 878
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Zebrafish
Match: CU929458.1 (pep chromosome:GRCz11:11:43363075:43368382:1 gene:ENSDARG00000114395.1 transcript:ENSDART00000179934.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CU929458.1)

HSP 1 Score: 75.8702 bits (185), Expect = 4.247e-23
Identity = 30/99 (30.30%), Postives = 65/99 (65.66%), Query Frame = 3 
Query:   27 NNIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNVIKE 323
            N + EH++ V  V +R+++  L  K  KCE+ ++++  +G++ S  G+  +   V+ ++++P P+++ ++++FLG AN+YR+F + FS +A PL  + +
Sbjct:  677 NTMKEHVEHVRIVLQRMIQHRLYAKLEKCEFHQTQIAFLGYVISAEGITMDDTKVQAVQRWPLPQNLKELQRFLGFANFYRRFIRGFSSIAAPLTAMTK 775

HSP 2 Score: 50.0618 bits (118), Expect = 4.247e-23
Identity = 31/106 (29.25%), Postives = 48/106 (45.28%), Query Frame = 1 
Query:  310 MLLKKQTKFKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIG----------EKDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIG 597
            M  +   K  W++  ++AF  L        ILR PN    FI+  +ASN  +G           K +  A+ SR L S E+NY    ++ LA+   +  +R +L G
Sbjct:  773 MTKRNSHKLSWSSEARQAFCDLKTQFTTAPILRHPNPDLPFIVEVDASNTGVGAVLSQRQGQPSKMYPCAFFSRKLTSAERNYDVGNRELLAMKLALEEWRHWLEG 878
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Xenopus
Match: anxa6 (annexin A6 [Source:Xenbase;Acc:XB-GENE-989741])

HSP 1 Score: 78.9518 bits (193), Expect = 4.285e-30
Identity = 38/90 (42.22%), Postives = 55/90 (61.11%), Query Frame = 3 
Query:   39 EHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPL 308
            EHL+ +++VF ++ +  L +KP KC    +EV  +GH      L+P+P  V  I ++P PK   QV  FLG + YYRKF  N+S VA+PL
Sbjct:  501 EHLQHLQRVFAQIQDAGLTLKPEKCHLAMAEVQYLGHRVGGGQLRPDPAKVEAICQWPIPKTQKQVLAFLGTSGYYRKFIPNYSTVAKPL 590

HSP 2 Score: 71.633 bits (174), Expect = 4.285e-30
Identity = 35/98 (35.71%), Postives = 54/98 (55.10%), Query Frame = 1 
Query:  340 WATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIGE---------KDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIGRKF 606
            W    + A + L ++L    +L  P+F + FIL T+ASNF +G          ++H  AY SR L   E  Y+TI+K+ LA+V+ +   + YL GR+F
Sbjct:  604 WTPECESAMNALKQALASSPVLAAPDFSRRFILQTDASNFGLGAVLSQVNTYGEEHPVAYLSRKLLPREAAYATIEKECLAIVWALQKLQPYLYGREF 701
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Xenopus
Match: ENSXETT00000006041.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:6:37317867:37322724:1 gene:ENSXETG00000003070.1 transcript:ENSXETT00000006041.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 87.4261 bits (215), Expect = 2.564e-23
Identity = 38/100 (38.00%), Postives = 61/100 (61.00%), Query Frame = 3 
Query:   30 NIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNVIKEAD 329
             + EH + + KVF RL +  LK+ P KC++ +  V  VGH+ S  G+  +P  +  +  +P P+ + ++R FLG   YYR+F K FS VAQPLN + +++
Sbjct:  488 TLEEHEQRLLKVFDRLEKEGLKLSPEKCQFCQPSVNYVGHVVSAEGIATDPSKIEAVSSWPKPRTITELRSFLGFCGYYRRFVKGFSKVAQPLNQLLQSN 587

HSP 2 Score: 40.0466 bits (92), Expect = 2.564e-23
Identity = 28/98 (28.57%), Postives = 45/98 (45.92%), Query Frame = 1 
Query:  340 WATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIG--------EKDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITN-FRQYLIGRKF 606
            W     +AFD+L   L    +L   +  K + L  +AS   +G        ++    A+ SRSL   E+NY   K + LAL + + +   +YL G +F
Sbjct:  616 WTEECDKAFDQLKYCLTHAPVLAYADATKPYTLHIDASREGLGGILYQEYNKELRPVAFISRSLSPSERNYPAHKLEFLALKWAVVDKLHEYLYGAEF 713
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Xenopus
Match: ENSXETT00000011934.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:3:51966053:51967075:1 gene:ENSXETG00000005538.1 transcript:ENSXETT00000011934.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 70.0922 bits (170), Expect = 2.596e-22
Identity = 35/103 (33.98%), Postives = 55/103 (53.40%), Query Frame = 1 
Query:  322 KQTKFKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIGE--------KDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIGRKF 606
            K     W    +EAF  L  SL +  +L  PNFKK F++ T+AS   +G         ++H  AY SR L   E  Y+ ++++ LA+ + + + R YL+GR+F
Sbjct:   72 KAGAITWTPEAEEAFLNLKASLCRYPVLIAPNFKKEFLVQTDASEVGLGAVLSQVVNGEEHPVAYLSRKLTPAECRYAIVERECLAIKWALESLRYYLLGRQF 174

HSP 2 Score: 54.299 bits (129), Expect = 2.596e-22
Identity = 23/71 (32.39%), Postives = 39/71 (54.93%), Query Frame = 3 
Query:  120 GKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNVIKEADQ 332
            G  E   +G++  R  ++P    +  I+++P P    QVR FLG+  YYR+F  NF+ +A PL  + +  +
Sbjct:    2 GLEEARYLGYVIGRGVVKPQVSKIEGIQQWPRPNSKKQVRAFLGIVGYYRRFVPNFASLASPLTDLTKGSK 72
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Xenopus
Match: ENSXETT00000035618.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:2:3692667:3694042:1 gene:ENSXETG00000010959.1 transcript:ENSXETT00000035618.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 75.0998 bits (183), Expect = 2.013e-20
Identity = 32/90 (35.56%), Postives = 53/90 (58.89%), Query Frame = 3 
Query:   60 KVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNVIKEAD 329
            KV  RL +  LK+   KC++ +S V  VGH+ S  G+  +P  +  +  +P P+ + ++R FLG   YYR+F + FS  A+PL+ + + D
Sbjct:    2 KVLDRLEKEGLKLSLDKCKFFQSSVTYVGHVVSAEGISTDPSKIEAVTSWPRPRTITELRSFLGFCGYYRRFVEGFSKTARPLSQLLQND 91

HSP 2 Score: 42.743 bits (99), Expect = 2.013e-20
Identity = 28/99 (28.28%), Postives = 47/99 (47.47%), Query Frame = 1 
Query:  337 KWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIG--------EKDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITN-FRQYLIGRKF 606
            +W +  + AF++L   L    +L   + +K + L  +AS   +G        +K    A+ SRSL   E+NY   K + LAL + + +    YL G +F
Sbjct:  119 RWTSECEGAFEQLKYCLTHAPVLAYADSRKPYTLHVDASREGLGGVLYQEHDQKLRPVAFISRSLSPTERNYPAHKLEFLALKWAVVDKLHDYLYGAEF 217
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Xenopus
Match: ENSXETT00000042189.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV460667.1:82848:87648:1 gene:ENSXETG00000017765.1 transcript:ENSXETT00000042189.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 72.0182 bits (175), Expect = 3.072e-20
Identity = 35/100 (35.00%), Postives = 60/100 (60.00%), Query Frame = 3 
Query:   30 NIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNF-SHVAQPLNVIKEA 326
            ++  H   V++   RL E  L  K  KC +   ++  +G+I S RG + +P  V  I+K+P P+    +++F+G ANYYR+F K+F SH+A  L++I++ 
Sbjct:  682 DLETHRSQVKEALSRLRENFLFAKLEKCTFEVPKISFLGYIISSRGFEMDPAKVSAIQKWPLPQSTKAIQRFIGFANYYRQFIKDFSSHIAPILSLIRKG 781

HSP 2 Score: 42.743 bits (99), Expect = 3.072e-20
Identity = 27/96 (28.12%), Postives = 41/96 (42.71%), Query Frame = 1 
Query:  340 WATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIGE----------KDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIG 597
            W     EAF  L ++ +  S+LR P     F +  +AS+   G           K H  AY S+   S E+NY    ++ LA+   +  +R  L G
Sbjct:  787 WPPVALEAFQSLKDAFISASVLRHPEPHLPFFIEVDASDVGAGAILSQRHSADGKLHPCAYFSKKFSSAEQNYDIGNRELLAVKLALEEWRHLLEG 882

HSP 3 Score: 21.9422 bits (45), Expect = 3.072e-20
Identity = 7/11 (63.64%), Postives = 11/11 (100.00%), Query Frame = 1 
Query:    7 LVYIDDIIIFT 39
            +VY+DDI+IF+
Sbjct:  670 VVYLDDILIFS 680
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. UniProt/SwissProt
Match: sp|P20825|POL2_DROME (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 9.670e-39
Identity = 39/93 (41.94%), Postives = 60/93 (64.52%), Query Frame = 3 
Query:   30 NIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPL 308
            ++ EHL  ++ VF +L + NLK++  KCE+ K E   +GHI +  G++PNP  V+ I  YP P    ++R FLGL  YYRKF  N++ +A+P+
Sbjct:  373 SLTEHLNSIQLVFTKLADANLKLQLDKCEFLKKEANFLGHIVTPDGIKPNPIKVKAIVSYPIPTKDKEIRAFLGLTGYYRKFIPNYADIAKPM 465

HSP 2 Score: 85.1149 bits (209), Expect = 9.670e-39
Identity = 44/103 (42.72%), Postives = 67/103 (65.05%), Query Frame = 1 
Query:  316 LKKQTKFKWATAQQ-EAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIG----EKDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIGRKFV 609
            LKK+TK      +  EAF+KL   +++  IL+ P+F+K F+L T+ASN A+G    +  H  ++ SR+L  HE NYS I+K+ LA+V+    FR YL+GR+F+
Sbjct:  469 LKKRTKIDTQKLEYIEAFEKLKALIIRDPILQLPDFEKKFVLTTDASNLALGAVLSQNGHPISFISRTLNDHELNYSAIEKELLAIVWATKTFRHYLLGRQFL 571

HSP 3 Score: 28.1054 bits (61), Expect = 9.670e-39
Identity = 10/13 (76.92%), Postives = 12/13 (92.31%), Query Frame = 1 
Query:    1 HALVYIDDIIIFT 39
            H LVY+DDIIIF+
Sbjct:  359 HCLVYLDDIIIFS 371
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. UniProt/SwissProt
Match: sp|P10394|POL4_DROME (Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaster OX=7227 GN=POL PE=4 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.064e-25
Identity = 33/82 (40.24%), Postives = 50/82 (60.98%), Query Frame = 3 
Query:   45 LKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFS 290
            LK++ +VF +  E NLK+ P KC +   EV  +GH  + +G+ P+ +    I+ YP P D D  R+F+   NYYR+F KNF+
Sbjct:  487 LKNLTEVFGKCREYNLKLHPEKCSFFMHEVTFLGHKCTDKGILPDDKKYDVIQNYPVPHDADSARRFVAFCNYYRRFIKNFA 568

HSP 2 Score: 64.6994 bits (156), Expect = 1.064e-25
Identity = 39/119 (32.77%), Postives = 62/119 (52.10%), Query Frame = 1 
Query:  274 FKKISRMSHNL*MLLKKQTKFKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIGE---KDH-----VTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIGRKF 606
             K  +  S ++  L KK   F+W    Q+AF  L   L+  ++L+ P+F K F + T+AS  A G    ++H       AYASR+    E N ST +++  A+ + I +FR Y+ G+ F
Sbjct:  564 IKNFADYSRHITRLCKKNVPFEWTDECQKAFIHLKSQLINPTLLQYPDFSKEFCITTDASKQACGAVLTQNHNGHQLPVAYASRAFTKGESNKSTTEQELAAIHWAIIHFRPYIYGKHF 682
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. UniProt/SwissProt
Match: sp|Q8I7P9|POL5_DROME (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 3.038e-25
Identity = 30/90 (33.33%), Postives = 57/90 (63.33%), Query Frame = 3 
Query:   42 HLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLN 311
            H K++  V   L + NL++   K  +  ++V  +G+I +  G++ +P+ VR I + P P  V ++++FLG+ +YYRKF ++++ VA+PL 
Sbjct:  294 HWKNLRLVLASLSKANLQVNLEKSHFLDTQVEFLGYIVTADGIKADPKKVRAISEMPPPTSVKELKRFLGMTSYYRKFIQDYAKVAKPLT 383

HSP 2 Score: 67.781 bits (164), Expect = 3.038e-25
Identity = 37/88 (42.05%), Postives = 51/88 (57.95%), Query Frame = 1 
Query:  358 EAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIGE--------KDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIG 597
            ++F+ L   L    IL  P F K F L T+ASN+AIG         +D   AY SRSL   E+NY+TI+K+ LA+++ + N R YL G
Sbjct:  411 QSFNDLKSILCSSEILAFPCFTKPFHLTTDASNWAIGAVLSQDDQGRDRPIAYISRSLNKTEENYATIEKEMLAIIWSLDNLRAYLYG 498
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. UniProt/SwissProt
Match: sp|P04323|POL3_DROME (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.078e-21
Identity = 48/133 (36.09%), Postives = 82/133 (61.65%), Query Frame = 3 
Query:   27 NNIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLN-VIKEADQI*MGNGSARS-FRQTQ*KLNETVNIKMPKF 419
             ++ EHL+ +  VF++L + NLK++  KCE+ K E   +GH+ +  G++PNPE +  I+KYP P    +++ FLGL  YYRKF  NF+ +A+P+   +K+  +I   N    S F++ +  ++E   +K+P F
Sbjct:  373 TSLDEHLQSLGLVFEKLAKANLKLQLDKCEFLKQETTFLGHVLTPDGIKPNPEKIEAIQKYPIPTKPKEIKAFLGLTGYYRKFIPNFADIAKPMTKCLKKNMKIDTTNPEYDSAFKKLKYLISEDPILKVPDF 505

HSP 2 Score: 77.7962 bits (190), Expect = 1.496e-15
Identity = 43/102 (42.16%), Postives = 60/102 (58.82%), Query Frame = 1 
Query:  316 LKKQTKFKWATAQQE-AFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIG----EKDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIGRKF 606
            LKK  K      + + AF KL   + +  IL+ P+F K F L T+AS+ A+G    +  H  +Y SR+L  HE NYSTI+K+ LA+V+    FR YL+GR F
Sbjct:  470 LKKNMKIDTTNPEYDSAFKKLKYLISEDPILKVPDFTKKFTLTTDASDVALGAVLSQDGHPLSYISRTLNEHEINYSTIEKELLAIVWATKTFRHYLLGRHF 571
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. UniProt/SwissProt
Match: sp|Q99315|YG31B_YEAST (Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-G PE=1 SV=3)

HSP 1 Score: 70.0922 bits (170), Expect = 9.052e-21
Identity = 34/92 (36.96%), Postives = 52/92 (56.52%), Query Frame = 3 
Query:   39 EHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNV 314
            EH K ++ V +RL   NL +K  KC++   E   +G+    + + P       I  +PTPK V Q ++FLG+ NYYR+F  N S +AQP+ +
Sbjct:  759 EHWKHLDTVLERLKNENLIVKKKKCKFASEETEFLGYSIGIQKIAPLQHKCAAIRDFPTPKTVKQAQRFLGMINYYRRFIPNCSKIAQPIQL 850

HSP 2 Score: 48.9062 bits (115), Expect = 9.052e-21
Identity = 34/102 (33.33%), Postives = 52/102 (50.98%), Query Frame = 1 
Query:  331 KFKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIG----EKDH------VTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIGRKF 606
            K +W   Q +A DKL ++L    +L   N K  + L T+AS   IG    E D+      V  Y S+SL+S +KNY   + + L ++  + +FR  L G+ F
Sbjct:  855 KSQWTEKQDKAIDKLKDALCNSPVLVPFNNKANYRLTTDASKDGIGAVLEEVDNKNKLVGVVGYFSKSLESAQKNYPAGELELLGIIKALHHFRYMLHGKHF 956

HSP 3 Score: 21.9422 bits (45), Expect = 9.052e-21
Identity = 7/10 (70.00%), Postives = 10/10 (100.00%), Query Frame = 1 
Query:   10 VYIDDIIIFT 39
            VY+DDI+IF+
Sbjct:  745 VYLDDILIFS 754
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. TrEMBL
Match: X1WXM3 (Reverse transcriptase domain-containing protein OS=Acyrthosiphon pisum OX=7029 PE=4 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 1.836e-39
Identity = 38/100 (38.00%), Postives = 70/100 (70.00%), Query Frame = 3 
Query:   30 NIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNVIKEAD 329
            ++ +H + +++VF RL E NLK++PSKC + + EV  +GH+ + +G++PNP+ +  + K+PTP +  +++ FLGL+ YYR+F  N+  +A+PL  + + D
Sbjct:  123 DLEDHSRKLKEVFDRLREANLKLQPSKCSFMRKEVNYLGHVITDKGVRPNPQKIDCVVKFPTPTNAKEIKSFLGLSGYYRRFVPNYGQIAKPLTSLLKKD 222

HSP 2 Score: 92.4337 bits (228), Expect = 1.836e-39
Identity = 48/106 (45.28%), Postives = 67/106 (63.21%), Query Frame = 1 
Query:  313 LLKKQTKFKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIG--------EKDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIGRKF 606
            LLKK   F W+   QE+FDKL   L K+ +L+ P+F++ F L  +ASNFAIG         KD   AYASR+L   E+NY+T +K+  A+V+G+  +R YL G+KF
Sbjct:  218 LLKKDVPFYWSDLCQESFDKLKAILTKEPLLQYPDFEQPFNLTCDASNFAIGCILSQGPIGKDLPIAYASRTLNKAEQNYNTTEKELCAIVWGVKQYRPYLYGQKF 323

HSP 3 Score: 23.0978 bits (48), Expect = 1.836e-39
Identity = 8/11 (72.73%), Postives = 10/11 (90.91%), Query Frame = 1 
Query:    4 ALVYIDDIIIF 36
            A VY+DDIII+
Sbjct:  110 AFVYLDDIIIY 120
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. TrEMBL
Match: A0A164SAA4 (Uncharacterized protein (Fragment) OS=Daphnia magna OX=35525 GN=APZ42_026526 PE=4 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 1.267e-36
Identity = 45/95 (47.37%), Postives = 60/95 (63.16%), Query Frame = 3 
Query:   27 NNIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLN 311
            +    HL+D+ +VF  L   NLK+K  KC++ K  V  +GHI S+ G+ P+PE + KI  YP PK  D +R FLGLA YYR+F K F  +AQPL 
Sbjct:  138 DTFEAHLEDIREVFNLLKIANLKLKLKKCQFIKHSVNYLGHIISKEGIAPDPEKIDKILNYPVPKSADDIRSFLGLAGYYRRFIKGFGSIAQPLT 232

HSP 2 Score: 77.411 bits (189), Expect = 1.267e-36
Identity = 39/100 (39.00%), Postives = 57/100 (57.00%), Query Frame = 1 
Query:  334 FKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIGE--------KDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIGRKFV 609
            F W    Q AF+ L   L  + IL  P+F K F+L T+A N+ IG          + V AYASR L+  E NY+T++K+ LA+++ I +FR YL+   F+
Sbjct:  244 FVWTDEDQVAFETLRTCLTTKPILAYPDFSKQFLLFTDACNYGIGAILSQIQDGNEVVIAYASRQLRKPELNYATVEKEALAVIFAIKSFRHYLLDEPFL 343

HSP 3 Score: 26.1794 bits (56), Expect = 1.267e-36
Identity = 9/13 (69.23%), Postives = 13/13 (100.00%), Query Frame = 1 
Query:    4 ALVYIDDIIIFTN 42
            ALVY+DD+IIF++
Sbjct:  126 ALVYLDDVIIFSD 138
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. TrEMBL
Match: A0A2S2QW04 (Retrovirus-related Pol polyprotein OS=Sipha flava OX=143950 GN=POL_31 PE=4 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.706e-36
Identity = 37/102 (36.27%), Postives = 67/102 (65.69%), Query Frame = 3 
Query:   30 NIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNVIKEADQI 335
            +I +H + ++ V  RL   NLK++PSKC + + EV  +GH+ +  G++P+P+ ++ + ++PTP +  QV+ FLGL+ YYR+F   +  +A+PL  + + D I
Sbjct:  231 SISDHSEKLKAVLDRLRTFNLKLQPSKCAFMRKEVNYLGHVITDEGVKPDPQKIKSVVEFPTPTNEKQVKSFLGLSGYYRRFVPGYGQIAKPLTSLLKKDTI 332

HSP 2 Score: 88.1965 bits (217), Expect = 2.706e-36
Identity = 46/106 (43.40%), Postives = 64/106 (60.38%), Query Frame = 1 
Query:  313 LLKKQTKFKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIG--------EKDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIGRKF 606
            LLKK T F W    QE+FDKL + L  + +L+ P+F + F L  +ASN+AIG          D    YASR+L   E+NY+T +K+  A+V+G+  FR YL G+KF
Sbjct:  326 LLKKDTIFNWTDLCQESFDKLKKILTTEPLLQYPDFTRKFNLTCDASNYAIGCVLLQGPIGTDPPITYASRTLNRAEQNYNTTEKELCAIVWGVKQFRPYLFGQKF 431

HSP 3 Score: 23.483 bits (49), Expect = 2.706e-36
Identity = 8/11 (72.73%), Postives = 10/11 (90.91%), Query Frame = 1 
Query:    4 ALVYIDDIIIF 36
            A VY+DDIII+
Sbjct:  218 AFVYLDDIIIY 228
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. TrEMBL
Match: A0A085MTL3 (Uncharacterized protein OS=Trichuris suis OX=68888 GN=M514_27263 PE=4 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 3.327e-36
Identity = 40/98 (40.82%), Postives = 68/98 (69.39%), Query Frame = 3 
Query:   36 HEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNVIKEAD 329
            ++H+K +  VF+R+    L ++  KC  G SEV  +GH+FS +G+QP+P  VR I  +P P +V++V++F+GLA+YYR+F  +F+ +A PL+ + + D
Sbjct:  218 NDHMKHLAVVFERISAAGLTLRGRKCRIGVSEVSFLGHVFSSKGMQPDPSKVRCIVNWPKPTNVNEVQRFIGLASYYRRFIAHFADIAAPLHSLTQKD 315

HSP 2 Score: 86.2705 bits (212), Expect = 3.327e-36
Identity = 42/102 (41.18%), Postives = 63/102 (61.76%), Query Frame = 1 
Query:  313 LLKKQTKFKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIG----EKDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIGRKF 606
            L +K   F W+   QE+F+ L E+L  + IL  P+    F L T+AS   +G    +++HV AYASR L+  E+NYS I+K+ LA+V+ +  FR YL+G +F
Sbjct:  311 LTQKDAPFVWSEKCQESFETLKEALSSEPILALPDCAVEFDLFTDASQHGLGAILEQRNHVIAYASRVLRPAERNYSVIEKECLAIVFAVKQFRHYLLGSRF 412
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. TrEMBL
Match: K7JAG9 (Reverse transcriptase domain-containing protein OS=Nasonia vitripennis OX=7425 PE=4 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 6.972e-36
Identity = 40/97 (41.24%), Postives = 64/97 (65.98%), Query Frame = 3 
Query:   27 NNIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNVI 317
            N + EH + V KVF+RL +  L+++  KC++   E+  +GHI  + G++P+P  +  +E++P P    ++RQFLGLA YYR+F  NF+  AQPLN +
Sbjct:  218 NTLEEHEEKVNKVFERLSDAGLRLQIDKCQFLTPEITYLGHIIDKNGVRPDPSKISAVEEFPPPTSQTKIRQFLGLAGYYRRFILNFAEKAQPLNSL 314

HSP 2 Score: 83.9593 bits (206), Expect = 6.972e-36
Identity = 45/106 (42.45%), Postives = 64/106 (60.38%), Query Frame = 1 
Query:  313 LLKKQTKFKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIGE--------KDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIGRKF 606
            LL+K  K++W  AQ+++F+ L ++L +  +L   +  K FIL T+AS+ AIG          DH  AY SRSL   EKNYST +K+ LA++  +  FR YL GR F
Sbjct:  314 LLQKNKKWEWTDAQKQSFETLKQALCEAPVLASVDLDKPFILTTDASDEAIGAVFSQGKPGDDHPVAYMSRSLNKVEKNYSTTEKECLAVIDAVDYFRHYLYGRHF 419
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Cavefish
Match: ENSAMXT00000052546.1 (pep primary_assembly:Astyanax_mexicanus-2.0:2:13031363:13036570:1 gene:ENSAMXG00000033629.1 transcript:ENSAMXT00000052546.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 86.2705 bits (212), Expect = 3.303e-31
Identity = 42/96 (43.75%), Postives = 60/96 (62.50%), Query Frame = 3 
Query:   30 NIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNVI 317
            +   H + +E VF RL    LK+KPSKC   K EV  +GHI S +G+Q + E VR +E +P PK V  +RQ LG  +YYR+F   F+ +A+PL+ +
Sbjct:  884 DFKSHCERLELVFNRLRHHGLKLKPSKCFLLKPEVKFLGHIISAKGVQVDAEKVRVLETWPIPKTVKDIRQVLGFMSYYRRFVPKFAQLAKPLHAL 979

HSP 2 Score: 63.1586 bits (152), Expect = 3.303e-31
Identity = 40/101 (39.60%), Postives = 53/101 (52.48%), Query Frame = 1 
Query:  340 WATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIGE--------KDHVTAYASRSLKSHE---KNYSTIKKKTLALVYGIT-NFRQYLIGRKF 606
            W+   Q AFDKL   LM   +L  P+F + FIL T+ S   +G          + V AYASR L+  E   KNYS  K + LAL + +T  FR +L+  KF
Sbjct:  995 WSGECQTAFDKLKGCLMSPPVLAYPDFTQPFILTTDGSLHGLGAVLSQKQGGTERVIAYASRGLRGSEKNDKNYSAFKLELLALKWAVTEKFRDFLMFAKF 1095

HSP 3 Score: 24.2534 bits (51), Expect = 3.303e-31
Identity = 9/11 (81.82%), Postives = 11/11 (100.00%), Query Frame = 1 
Query:    7 LVYIDDIIIFT 39
            LVY+DDIIIF+
Sbjct:  872 LVYLDDIIIFS 882
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Cavefish
Match: ENSAMXT00000038088.1 (pep primary_assembly:Astyanax_mexicanus-2.0:23:13869500:13874588:-1 gene:ENSAMXG00000037864.1 transcript:ENSAMXT00000038088.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 87.8113 bits (216), Expect = 1.003e-30
Identity = 36/90 (40.00%), Postives = 63/90 (70.00%), Query Frame = 3 
Query:   42 HLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLN 311
            H++ +E+VF+RL    LK++  KC+  + +V  +GH+ +++G+ P+PE V  ++++ TP  + +VR FLGLA YYR+F   F+ +A+PLN
Sbjct:  503 HVQHLEQVFERLSRYGLKLRSDKCKLFRRQVKFLGHVVNKQGVHPDPEKVAAVQEWATPTTIREVRAFLGLAGYYRRFIDGFAKLARPLN 592

HSP 2 Score: 63.929 bits (154), Expect = 1.003e-30
Identity = 40/102 (39.22%), Postives = 56/102 (54.90%), Query Frame = 1 
Query:  340 WATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIGE--------KDHVTAYASRSLKSHEK---NYSTIKKKTLALVYGIT-NFRQYLIGRKFV 609
            W    Q AF+ L   L++  IL   +F   FI+ T+AS+  +G         ++ V AYASRSL   EK   NYS+ K + LAL + +T  FR YL+G KF+
Sbjct:  611 WTPECQNAFECLKSCLLQAPILAYADFSAPFIVYTDASSQGLGAVLSQMQDGRERVIAYASRSLHPSEKNDANYSSFKLELLALKWAVTEKFRDYLMGSKFM 712
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Cavefish
Match: ENSAMXT00000048059.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001896.1:45492:48938:1 gene:ENSAMXG00000034382.1 transcript:ENSAMXT00000048059.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 89.3521 bits (220), Expect = 3.510e-30
Identity = 39/94 (41.49%), Postives = 62/94 (65.96%), Query Frame = 3 
Query:   30 NIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLN 311
            + + HL  ++ VF RL +  LK+KP KC+  +S V  +GH+ S  G+ P+P+ V  ++++  PK + ++R FLGLA YYR+F K FS +A PL+
Sbjct:  302 DFNSHLDRLDFVFSRLSQQGLKLKPDKCKLLRSSVQYLGHVVSDAGIAPDPDKVMAVQQWRVPKSITELRAFLGLAGYYRRFVKGFSQIAAPLH 395

HSP 2 Score: 60.4622 bits (145), Expect = 3.510e-30
Identity = 36/103 (34.95%), Postives = 58/103 (56.31%), Query Frame = 1 
Query:  334 FKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIGE--------KDHVTAYASRSLKSHEK---NYSTIKKKTLALVYGIT-NFRQYLIGRKF 606
            +KW+   + AF+ L + L++  +L   +F   F+L T+AS+  +G         ++ V AYASR L+  E+   NYS+ K + LAL + IT  F +YL+G  F
Sbjct:  415 WKWSNECETAFESLKQGLVQAPVLAFADFTLPFLLYTDASHRGLGAVLSQKQDGQEKVIAYASRGLQGAERNDANYSSFKLELLALKWAITEKFHEYLLGSDF 517
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Cavefish
Match: ENSAMXT00000051531.1 (pep primary_assembly:Astyanax_mexicanus-2.0:16:23481988:23484356:-1 gene:ENSAMXG00000035923.1 transcript:ENSAMXT00000051531.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 87.4261 bits (215), Expect = 8.383e-30
Identity = 39/94 (41.49%), Postives = 61/94 (64.89%), Query Frame = 3 
Query:   30 NIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLN 311
            + + HL  ++ VF RL +  LK+KP KC+  +S V  +GH+ S  G+ P+P+ V  ++++  PK + ++R FLGLA YYR+F K FS  A PL+
Sbjct:   41 DFNSHLDRLDFVFSRLSQQGLKLKPDKCKLLRSSVQYLGHVVSDAGIAPDPDKVTAVQQWRVPKFITELRAFLGLAGYYRRFVKGFSQTAAPLH 134

HSP 2 Score: 61.2326 bits (147), Expect = 8.383e-30
Identity = 37/103 (35.92%), Postives = 58/103 (56.31%), Query Frame = 1 
Query:  334 FKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIGE--------KDHVTAYASRSLKSHEK---NYSTIKKKTLALVYGIT-NFRQYLIGRKF 606
            +KWA   + AF+ L + L++  +L   +F   F+L T+AS+  +G         ++ V AYASR L+  E+   NYS+ K + LAL + IT  F +YL+G  F
Sbjct:  154 WKWANECETAFESLKQGLVQAPVLAFADFTLPFLLYTDASHRGLGAVLSQKQDGQEKVIAYASRGLQGAERNDANYSSFKWELLALKWAITEKFHEYLLGSDF 256
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Cavefish
Match: ENSAMXT00000040218.1 (pep primary_assembly:Astyanax_mexicanus-2.0:3:9815041:9817149:1 gene:ENSAMXG00000041896.1 transcript:ENSAMXT00000040218.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 80.1073 bits (196), Expect = 6.143e-29
Identity = 36/91 (39.56%), Postives = 58/91 (63.74%), Query Frame = 3 
Query:   39 EHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLN 311
            EH++ +E V +RL ET LKIK  KC + + +V  +GH  S  G+  +P+ V  ++++P P  +  +R FLG  +YYR+F + FS +A PL+
Sbjct:   60 EHVERLETVLRRLQETGLKIKIEKCHFLQEKVKFLGHQVSAEGIATDPDKVEAVKQWPVPTTLKALRSFLGFCSYYRRFVQGFSKIAGPLH 150

HSP 2 Score: 63.1586 bits (152), Expect = 6.143e-29
Identity = 41/101 (40.59%), Postives = 56/101 (55.45%), Query Frame = 1 
Query:  340 WATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIGE--------KDHVTAYASRSLKSHE---KNYSTIKKKTLALVYGIT-NFRQYLIGRKF 606
            W+T  Q +FD L E L+   +L   +F   F + T+AS+  +G         K  V AYASR L+  E   KNYS++K + LAL + IT  FR YL+G KF
Sbjct:  173 WSTECQTSFDVLKEKLVSAPVLGFADFTCPFTVETDASSHGLGAVLCQQQDGKRRVIAYASRRLRKAEQNDKNYSSMKLELLALKWAITEKFRGYLLGAKF 273

HSP 3 Score: 22.3274 bits (46), Expect = 6.143e-29
Identity = 7/11 (63.64%), Postives = 11/11 (100.00%), Query Frame = 1 
Query:    7 LVYIDDIIIFT 39
            LVY+DDI++F+
Sbjct:   45 LVYLDDILVFS 55
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Nematostella
Match: EDO33875 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SR01])

HSP 1 Score: 46.595 bits (109), Expect = 1.797e-7
Identity = 20/80 (25.00%), Postives = 47/80 (58.75%), Query Frame = 3 
Query:   24 HNNIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANY 263
            H++  EHL+ +E++ + +  +  K    K ++    V  +GH+  + G++P PE +  I ++ TP + +++R+F+G+  +
Sbjct:    7 HSSFEEHLRGIERMLQAVRASGFK-SIKKSQFATRSVKFLGHVIDQNGVRPQPEKL-DIRQWETPTNEEELRKFIGVCTF 84
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Medaka
Match: ENSORLT00000037716.1 (pep primary_assembly:ASM223467v1:20:9739400:9742664:-1 gene:ENSORLG00000027308.1 transcript:ENSORLT00000037716.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 90.8929 bits (224), Expect = 4.538e-32
Identity = 41/97 (42.27%), Postives = 63/97 (64.95%), Query Frame = 3 
Query:   21 RHNNIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLN 311
            +H+    HL   ++VF RL +  LK++P KC   + EV  +GHI  R G++P+PE V+ ++ +P P  +  VR FLGLA YYR+F   F+++A+PLN
Sbjct:  281 QHSLTEGHL---DEVFGRLWQHGLKLRPDKCRLFQQEVKFLGHIVDRSGVRPDPEKVKAVQDWPIPSTIKGVRAFLGLAGYYRRFVAGFANIARPLN 374

HSP 2 Score: 65.0846 bits (157), Expect = 4.538e-32
Identity = 39/101 (38.61%), Postives = 57/101 (56.44%), Query Frame = 1 
Query:  340 WATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIGE--------KDHVTAYASRSLKSHEK---NYSTIKKKTLALVYGIT-NFRQYLIGRKF 606
            W    + +FD L E+L +  IL   +F K F++ T+AS+  +G         ++ V AYASRSL   E+   NYS+ K + LAL + IT  F+ YL+G KF
Sbjct:  393 WTPDCKASFDALKEALTQAPILAYADFNKPFVVYTDASHHGLGAVLAQVQEGRERVIAYASRSLHPSERNDANYSSFKLELLALKWAITEKFKDYLMGAKF 493
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Medaka
Match: ENSORLT00000041940.1 (pep primary_assembly:ASM223467v1:3:114973:116062:1 gene:ENSORLG00000022411.1 transcript:ENSORLT00000041940.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 82.0333 bits (201), Expect = 1.697e-29
Identity = 34/93 (36.56%), Postives = 60/93 (64.52%), Query Frame = 3 
Query:   42 HLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNVIK 320
            H++D+  VF+RL    L +  SKC + + E+  +GH+ S +G++ +PE  + +  +PTP D+  +++FLGLA +Y KF  +F+ +  PLN +K
Sbjct:   53 HMEDLTAVFRRLKAAGLSLNMSKCHFFRRELRFLGHLVSEKGVEVDPEKTQAVTDFPTPTDIKSLQRFLGLAGWYHKFIPHFADITAPLNHLK 145

HSP 2 Score: 65.4698 bits (158), Expect = 1.697e-29
Identity = 38/106 (35.85%), Postives = 59/106 (55.66%), Query Frame = 1 
Query:  313 LLKKQTKFKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIG--------EKDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIGRKF 606
            L KK  ++ W    Q++ + L E+L    IL  P   + F + T+AS   +G        + + V AYASR L+  EKNYST +K+ LA+V+ +  +R YL G++F
Sbjct:  144 LKKKGVEWNWTKECQQSVEMLKEALKTPPILSQP-VSRAFQVHTDASEVGLGAILTQDTPQGECVIAYASRVLRGPEKNYSTSEKECLAVVWAVEKWRHYLEGQEF 248
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Medaka
Match: ENSORLT00000044989.1 (pep primary_assembly:ASM223467v1:1:17572028:17576089:-1 gene:ENSORLG00000028051.1 transcript:ENSORLT00000044989.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 80.8777 bits (198), Expect = 5.395e-29
Identity = 37/90 (41.11%), Postives = 58/90 (64.44%), Query Frame = 3 
Query:   42 HLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLN 311
            HL  ++ VF+RL ET LK+K  KC + +S V  +GH  S  G+  +P+ V  ++++P P  V ++R FLG  +YYR+F + FS +A PL+
Sbjct:  438 HLTRLQTVFQRLRETGLKVKVEKCNFLQSSVRFLGHQISADGIGTDPDKVAAVKQWPVPASVKELRSFLGFCSYYRRFLQGFSQLAGPLH 527

HSP 2 Score: 63.929 bits (154), Expect = 5.395e-29
Identity = 39/101 (38.61%), Postives = 56/101 (55.45%), Query Frame = 1 
Query:  340 WATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIGE--------KDHVTAYASRSLKSHEK---NYSTIKKKTLALVYGIT-NFRQYLIGRKF 606
            W    Q++F++L E L    +L   NF   FIL T+AS+  +G         +  V AYASR L+  EK   NYS++K + LAL + ++  FR YL+G KF
Sbjct:  550 WTAECQDSFEELKERLTSAPLLGYANFSLPFILETDASSLGLGAVLYQIQDGEKRVIAYASRRLRGAEKNDRNYSSMKLELLALKWAVSEKFRGYLLGAKF 650

HSP 3 Score: 21.1718 bits (43), Expect = 5.395e-29
Identity = 6/11 (54.55%), Postives = 11/11 (100.00%), Query Frame = 1 
Query:    7 LVYIDDIIIFT 39
            LVY+DDI++++
Sbjct:  422 LVYLDDILVYS 432
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Medaka
Match: ENSORLT00000026768.1 (pep primary_assembly:ASM223467v1:14:3409724:3412345:1 gene:ENSORLG00000026986.1 transcript:ENSORLT00000026768.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 78.5666 bits (192), Expect = 3.447e-28
Identity = 34/98 (34.69%), Postives = 64/98 (65.31%), Query Frame = 3 
Query:   39 EHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNVIKEADQ 332
            EH++ +++V +RL E+ L++K SKC++ + EV+ +GH   + GL P P  V+ +++ P+P  V +++ +LGL N+Y +F  N S +  PL+ +   D+
Sbjct:  154 EHIQILDQVLQRLEESGLRLKRSKCQFLEKEVIFLGHKVDKTGLHPLPAKVKAVQEAPSPTSVTELKAYLGLLNFYNRFLPNLSTLLAPLHKLLRKDE 251

HSP 2 Score: 64.6994 bits (156), Expect = 3.447e-28
Identity = 35/108 (32.41%), Postives = 58/108 (53.70%), Query Frame = 1 
Query:  313 LLKKQTKFKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIGE---------KDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIGRKFV 609
            LL+K  K+ WA  Q+  F    E L    +L   + +K  IL  +AS + +G          ++    +ASR+L + E+NYS + K+ L +++G+  F +YL GRKF+
Sbjct:  246 LLRKDEKWVWAPDQERVFKLSKELLQSDRVLVHYDERKELILSCDASPYGVGAVLAHRMPDGQEKPIGFASRTLGAAERNYSQLDKEGLPVIFGVQYFHKYLYGRKFI 353
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Medaka
Match: ENSORLT00000037270.1 (pep primary_assembly:ASM223467v1:9:1559612:1561330:1 gene:ENSORLG00000027443.1 transcript:ENSORLT00000037270.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 77.7962 bits (190), Expect = 1.194e-27
Identity = 34/93 (36.56%), Postives = 58/93 (62.37%), Query Frame = 3 
Query:   30 NIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPL 308
            NI +H + + ++  R+ + NLK+  SKC+   +E+  +GH+ S +GL+P+PE V+ I+  P P+D   +++F GL  Y  KF  N S ++ PL
Sbjct:  350 NIEQHDRRLRQLLDRIRKINLKLNKSKCKIRMTEITYIGHVLSEQGLKPDPEKVQAIQNMPAPEDKAALQRFTGLLQYLSKFIPNLSDISAPL 442

HSP 2 Score: 63.5438 bits (153), Expect = 1.194e-27
Identity = 33/99 (33.33%), Postives = 56/99 (56.57%), Query Frame = 1 
Query:  316 LKKQTKFKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIG----EKDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIGR 600
            L+   ++ W T QQ++F+KL   + +  +L+  +  K   L  +AS+  +G    ++    AY SR+L   +K Y+ I+K+ LA+V+G   F QYL GR
Sbjct:  445 LEGSVEWHWETEQQQSFEKLKTLVSQAPVLKYYDVNKPVTLSVDASSEGLGAVLLQEGQPVAYGSRALTDCQKRYAQIEKELLAIVFGCEKFHQYLYGR 543
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Planmine SMEST
Match: SMESG000080881.1 (SMESG000080881.1)

HSP 1 Score: 167.933 bits (424), Expect = 8.133e-48
Identity = 81/114 (71.05%), Postives = 93/114 (81.58%), Query Frame = 3 
Query:   27 NNIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNV-IKEADQI*MGNGSARSF 365
            N   EHLKDVE VF+RLL+ NLKIKPSKCEWGKSEVV VGHI SR GLQPNPENV+KI++YP P++VDQVRQFLGLA+YYRKF K+FSHVAQPLNV +K+  Q   G     +F
Sbjct: 3542 NTFKEHLKDVETVFRRLLKANLKIKPSKCEWGKSEVVYVGHIISRNGLQPNPENVKKIKEYPIPENVDQVRQFLGLASYYRKFIKSFSHVAQPLNVLLKKQTQFKWGKSQQEAF 3655

HSP 2 Score: 132.88 bits (333), Expect = 1.327e-35
Identity = 95/209 (45.45%), Postives = 123/209 (58.85%), Query Frame = 1 
Query:    1 HALVYIDDIIIFTNI*KMWKRYSRDY*RQT*K*SRQSASGENQKLCVW----VIYFQDEVC-NQIQKT*EKSRNIRP---PRMWIKFDNFLD*PITTENF-KKISRMSHNL*MLLKKQTKFKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAI---------GEKDHVTAYASRSLKSHEKNYSTIKKKTLALVYGIT 573
            HALVYIDDIIIF+N    +K + +D      +  + +   +  K C W    V+Y    +  N +Q   E  + I+    P    +   FL        F K  S ++  L +LLKKQT+FKW  +QQEAF  L +SL+K+ ILRCPNF+  FILMT+ASNFA+         GEKDHV AYASR+LK HEKNYSTI+K+TLAL YGI 
Sbjct: 3529 HALVYIDDIIIFSN---TFKEHLKDVETVFRRLLKANLKIKPSK-CEWGKSEVVYVGHIISRNGLQPNPENVKKIKEYPIPENVDQVRQFLGLASYYRKFIKSFSHVAQPLNVLLKKQTQFKWGKSQQEAFKLLKKSLIKKPILRCPNFRIPFILMTDASNFALGAVLGQQVEGEKDHVIAYASRTLKPHEKNYSTIEKETLALAYGIA 3733
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Planmine SMEST
Match: SMESG000057446.1 (SMESG000057446.1)

HSP 1 Score: 163.696 bits (413), Expect = 2.428e-46
Identity = 106/221 (47.96%), Postives = 130/221 (58.82%), Query Frame = 1 
Query:    1 HALVYIDDIIIFTNI*KMWKRYSRDY*RQT*K*SRQSASGENQKLCVW----VIYFQDEVC-NQIQKT*EKSRNIRP---PRMWIKFDNFLD*PITTENF-KKISRMSHNL*MLLKKQTKFKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIG---------EKDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIGRKFV 609
            HALVYIDD+IIF+N  K   +      R+  K   +    +    C W    V+Y    +  N +Q   E  R I+    P    +   FL        F K  S ++  L +LLKKQ + KW  AQQEAF+ L ESL+K+ ILRCPNF+  FILMT+ASNFA G         EKDHV AYASR+LK HEKNYSTI+K+TLALVYGI  FRQYL+GRKFV
Sbjct: 2010 HALVYIDDVIIFSNTFKEHLKDVETVFRRLLKAKLKIKPSK----CEWGKSEVVYVGHIISRNGLQPNPENVRKIKEYPIPENVDQVRQFLGLASYYRKFIKSFSHVAQLLNVLLKKQIQSKWGKAQQEAFELLKESLIKKPILRCPNFRIPFILMTDASNFAFGAVLGQQVEGEKDHVIAYASRTLKPHEKNYSTIEKETLALVYGIATFRQYLVGRKFV 2226

HSP 2 Score: 161.384 bits (407), Expect = 1.307e-45
Identity = 76/97 (78.35%), Postives = 85/97 (87.63%), Query Frame = 3 
Query:   27 NNIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNVI 317
            N   EHLKDVE VF+RLL+  LKIKPSKCEWGKSEVV VGHI SR GLQPNPENVRKI++YP P++VDQVRQFLGLA+YYRKF K+FSHVAQ LNV+
Sbjct: 2023 NTFKEHLKDVETVFRRLLKAKLKIKPSKCEWGKSEVVYVGHIISRNGLQPNPENVRKIKEYPIPENVDQVRQFLGLASYYRKFIKSFSHVAQLLNVL 2119
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Planmine SMEST
Match: SMESG000057446.1 (SMESG000057446.1)

HSP 1 Score: 163.696 bits (413), Expect = 2.476e-46
Identity = 106/221 (47.96%), Postives = 130/221 (58.82%), Query Frame = 1 
Query:    1 HALVYIDDIIIFTNI*KMWKRYSRDY*RQT*K*SRQSASGENQKLCVW----VIYFQDEVC-NQIQKT*EKSRNIRP---PRMWIKFDNFLD*PITTENF-KKISRMSHNL*MLLKKQTKFKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIG---------EKDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIGRKFV 609
            HALVYIDD+IIF+N  K   +      R+  K   +    +    C W    V+Y    +  N +Q   E  R I+    P    +   FL        F K  S ++  L +LLKKQ + KW  AQQEAF+ L ESL+K+ ILRCPNF+  FILMT+ASNFA G         EKDHV AYASR+LK HEKNYSTI+K+TLALVYGI  FRQYL+GRKFV
Sbjct: 2020 HALVYIDDVIIFSNTFKEHLKDVETVFRRLLKAKLKIKPSK----CEWGKSEVVYVGHIISRNGLQPNPENVRKIKEYPIPENVDQVRQFLGLASYYRKFIKSFSHVAQLLNVLLKKQIQSKWGKAQQEAFELLKESLIKKPILRCPNFRIPFILMTDASNFAFGAVLGQQVEGEKDHVIAYASRTLKPHEKNYSTIEKETLALVYGIATFRQYLVGRKFV 2236

HSP 2 Score: 161.384 bits (407), Expect = 1.359e-45
Identity = 76/97 (78.35%), Postives = 85/97 (87.63%), Query Frame = 3 
Query:   27 NNIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNVI 317
            N   EHLKDVE VF+RLL+  LKIKPSKCEWGKSEVV VGHI SR GLQPNPENVRKI++YP P++VDQVRQFLGLA+YYRKF K+FSHVAQ LNV+
Sbjct: 2033 NTFKEHLKDVETVFRRLLKAKLKIKPSKCEWGKSEVVYVGHIISRNGLQPNPENVRKIKEYPIPENVDQVRQFLGLASYYRKFIKSFSHVAQLLNVL 2129
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Planmine SMEST
Match: SMESG000057446.1 (SMESG000057446.1)

HSP 1 Score: 163.31 bits (412), Expect = 2.694e-46
Identity = 106/221 (47.96%), Postives = 130/221 (58.82%), Query Frame = 1 
Query:    1 HALVYIDDIIIFTNI*KMWKRYSRDY*RQT*K*SRQSASGENQKLCVW----VIYFQDEVC-NQIQKT*EKSRNIRP---PRMWIKFDNFLD*PITTENF-KKISRMSHNL*MLLKKQTKFKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIG---------EKDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIGRKFV 609
            HALVYIDD+IIF+N  K   +      R+  K   +    +    C W    V+Y    +  N +Q   E  R I+    P    +   FL        F K  S ++  L +LLKKQ + KW  AQQEAF+ L ESL+K+ ILRCPNF+  FILMT+ASNFA G         EKDHV AYASR+LK HEKNYSTI+K+TLALVYGI  FRQYL+GRKFV
Sbjct: 2020 HALVYIDDVIIFSNTFKEHLKDVETVFRRLLKAKLKIKPSK----CEWGKSEVVYVGHIISRNGLQPNPENVRKIKEYPIPENVDQVRQFLGLASYYRKFIKSFSHVAQLLNVLLKKQIQSKWGKAQQEAFELLKESLIKKPILRCPNFRIPFILMTDASNFAFGAVLGQQVEGEKDHVIAYASRTLKPHEKNYSTIEKETLALVYGIATFRQYLVGRKFV 2236

HSP 2 Score: 161.384 bits (407), Expect = 1.219e-45
Identity = 76/97 (78.35%), Postives = 85/97 (87.63%), Query Frame = 3 
Query:   27 NNIHEHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNVI 317
            N   EHLKDVE VF+RLL+  LKIKPSKCEWGKSEVV VGHI SR GLQPNPENVRKI++YP P++VDQVRQFLGLA+YYRKF K+FSHVAQ LNV+
Sbjct: 2033 NTFKEHLKDVETVFRRLLKAKLKIKPSKCEWGKSEVVYVGHIISRNGLQPNPENVRKIKEYPIPENVDQVRQFLGLASYYRKFIKSFSHVAQLLNVL 2129
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Planmine SMEST
Match: SMESG000066603.1 (SMESG000066603.1)

HSP 1 Score: 160.229 bits (404), Expect = 3.571e-45
Identity = 79/121 (65.29%), Postives = 95/121 (78.51%), Query Frame = 1 
Query:  274 FKKISRMSHNL*MLLKKQTKFKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNFAIG---------EKDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIGRKFV 609
             K  S ++  L +LLKKQ+KF W  +QQEAF+ L ESL KQ ILRCP+FKK FI+MT+ASNFA+G         EKDHV AYASR+LKSHEKNYSTI+K+TLALVYGIT FR+YL+GRKF+
Sbjct: 1201 IKDFSHIAQPLNVLLKKQSKFVWKKSQQEAFELLKESLTKQPILRCPDFKKHFIVMTDASNFALGAVLGQQVEGEKDHVIAYASRTLKSHEKNYSTIEKETLALVYGITTFRKYLVGRKFL 1321

HSP 2 Score: 139.428 bits (350), Expect = 6.007e-38
Identity = 66/93 (70.97%), Postives = 75/93 (80.65%), Query Frame = 3 
Query:   39 EHLKDVEKVFKRLLETNLKIKPSKCEWGKSEVVCVGHIFSRRGLQPNPENVRKIEKYPTPKDVDQVRQFLGLANYYRKF*KNFSHVAQPLNVI 317
            EHL DVE VFKR  + NLKIKPSKCEWGKSEVV VGH  SR GLQ NP+NV KI  YP P ++DQ+RQFL  A+YYRKF K+FSH+AQPLNV+
Sbjct: 1122 EHLIDVEVVFKRRRKANLKIKPSKCEWGKSEVVYVGHFISRNGLQSNPDNVIKIRDYPVPNNLDQIRQFLISASYYRKFIKDFSHIAQPLNVL 1214
The following BLAST results are available for this feature:
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
BX890543.17.136e-2435.87pep chromosome:GRCz11:14:42224893:42231293:-1 gene... [more]
CR354395.28.475e-2430.30pep chromosome:GRCz11:12:13553730:13556536:-1 gene... [more]
BX571665.11.268e-2330.30pep chromosome:GRCz11:17:35431724:35437222:1 gene:... [more]
CR533578.31.311e-2330.30pep chromosome:GRCz11:16:23466094:23471387:-1 gene... [more]
CU929458.14.247e-2330.30pep chromosome:GRCz11:11:43363075:43368382:1 gene:... [more]
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BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
anxa64.285e-3042.22annexin A6 [Source:Xenbase;Acc:XB-GENE-989741][more]
ENSXETT00000006041.12.564e-2338.00pep primary_assembly:Xenopus_tropicalis_v9.1:6:373... [more]
ENSXETT00000011934.12.596e-2233.98pep primary_assembly:Xenopus_tropicalis_v9.1:3:519... [more]
ENSXETT00000035618.12.013e-2035.56pep primary_assembly:Xenopus_tropicalis_v9.1:2:369... [more]
ENSXETT00000042189.13.072e-2035.00pep primary_assembly:Xenopus_tropicalis_v9.1:KV460... [more]
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BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Retrovirus-related Pol polyprotein from transposon vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P20825|POL2_DROME9.670e-3941.94Retrovirus-related Pol polyprotein from transposon... [more]
sp|P10394|POL4_DROME1.064e-2540.24Retrovirus-related Pol polyprotein from transposon... [more]
sp|Q8I7P9|POL5_DROME3.038e-2533.33Retrovirus-related Pol polyprotein from transposon... [more]
sp|P04323|POL3_DROME1.078e-2136.09Retrovirus-related Pol polyprotein from transposon... [more]
sp|Q99315|YG31B_YEAST9.052e-2136.96Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomy... [more]
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BLAST of Retrovirus-related Pol polyprotein from transposon vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
X1WXM31.836e-3938.00Reverse transcriptase domain-containing protein OS... [more]
A0A164SAA41.267e-3647.37Uncharacterized protein (Fragment) OS=Daphnia magn... [more]
A0A2S2QW042.706e-3636.27Retrovirus-related Pol polyprotein OS=Sipha flava ... [more]
A0A085MTL33.327e-3640.82Uncharacterized protein OS=Trichuris suis OX=68888... [more]
K7JAG96.972e-3641.24Reverse transcriptase domain-containing protein OS... [more]
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BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000052546.13.303e-3143.75pep primary_assembly:Astyanax_mexicanus-2.0:2:1303... [more]
ENSAMXT00000038088.11.003e-3040.00pep primary_assembly:Astyanax_mexicanus-2.0:23:138... [more]
ENSAMXT00000048059.13.510e-3041.49pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000051531.18.383e-3041.49pep primary_assembly:Astyanax_mexicanus-2.0:16:234... [more]
ENSAMXT00000040218.16.143e-2939.56pep primary_assembly:Astyanax_mexicanus-2.0:3:9815... [more]
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BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO338751.797e-725.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000037716.14.538e-3242.27pep primary_assembly:ASM223467v1:20:9739400:974266... [more]
ENSORLT00000041940.11.697e-2936.56pep primary_assembly:ASM223467v1:3:114973:116062:1... [more]
ENSORLT00000044989.15.395e-2941.11pep primary_assembly:ASM223467v1:1:17572028:175760... [more]
ENSORLT00000026768.13.447e-2834.69pep primary_assembly:ASM223467v1:14:3409724:341234... [more]
ENSORLT00000037270.11.194e-2736.56pep primary_assembly:ASM223467v1:9:1559612:1561330... [more]
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BLAST of Retrovirus-related Pol polyprotein from transposon vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000080881.18.133e-4871.05SMESG000080881.1[more]
SMESG000057446.12.428e-4647.96SMESG000057446.1[more]
SMESG000057446.12.476e-4647.96SMESG000057446.1[more]
SMESG000057446.12.694e-4647.96SMESG000057446.1[more]
SMESG000066603.13.571e-4565.29SMESG000066603.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016067 ID=SMED30016067|Name=Retrovirus-related Pol polyprotein from transposon|organism=Schmidtea mediterranea sexual|type=transcript|length=609bp
CACGCATTAGTATACATCGACGACATAATAATATTCACGAACATTTGAAA
GATGTGGAAAAGGTATTCAAGAGATTATTAGAGACAAACTTAAAAATAAA
GCCGTCAAAGTGCGAGTGGGGAAAATCAGAAGTTGTGTGTGTGGGTCATA
TATTTTCAAGACGAGGTTTGCAACCAAATCCAGAAAACGTGAGAAAAATC
GAGAAATATCCGACCCCCAAGGATGTGGATCAAGTTCGACAATTTCTTGG
ATTAGCCAATTACTACAGAAAATTTTAAAAAAATTTCTCGCATGTCGCAC
AACCTTTAAATGTTATTAAAGAAGCAGACCAAATTTAAATGGGCAACGGC
TCAGCAAGAAGCTTTCGACAAACTCAATGAAAGCTTAATGAAACAGTCAA
TATTAAGATGCCCAAATTTTAAAAAGCTATTTATATTAATGACGAACGCC
AGTAATTTTGCAATAGGTGAAAAAGATCATGTTACTGCTTATGCCAGCCG
ATCATTAAAATCTCATGAGAAGAATTATTCAACAATTAAGAAAAAAACGT
TAGCTTTAGTTTATGGAATAACTAATTTCCGACAATACTTAATTGGAAGA
AAATTTGTC
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protein sequence of SMED30016067-orf-1

>SMED30016067-orf-1 ID=SMED30016067-orf-1|Name=SMED30016067-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=100bp
MLLKKQTKFKWATAQQEAFDKLNESLMKQSILRCPNFKKLFILMTNASNF
AIGEKDHVTAYASRSLKSHEKNYSTIKKKTLALVYGITNFRQYLIGRKFV
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0004190aspartic-type endopeptidase activity
GO:0008270zinc ion binding
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
GO:0015074DNA integration