SMED30016022
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30016022 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 19
Alignments
SMED30016022 aligns in the following genomic locations:
Homology
BLAST of SMED30016022 vs. Planmine SMEST
Match: SMESG000053405.1 (SMESG000053405.1) HSP 1 Score: 855.899 bits (2210), Expect = 0.000e+0 Identity = 480/510 (94.12%), Postives = 481/510 (94.31%), Query Frame = 2 Query: 65 MRHIHLIMATLVFNQFVDIFQGFLIGHLKENFRNKMEIDIFIYFKPISNRRLLLAIDKNNNSFTVLTSKYFHKDFQLIIDFNGLNYSVTSQSAIDLVFDEEIFKINLQLSIGNNNVYELGSTRILLENIFNHTNPTSFDDLLNAYNFHQMIDKTIIFVGNIDNIQLNGAINILFEEIIGCIQGFYVNEYKTKFIQAGDYFYPYCSKRATESICEERNCRNRSIDYYTLCCSLSGINAGEHCYKIRTEQQKSSYRISAEDSNIRIAKFAWRPKLLENECLIQIVTKNNSLITIYKNPLGEILLVQQNRQSEIWYKKSLYKLQSNRTHILXXXXXXXXXXXXXXXXXXXXXFYFPLDASAGYVNVGWTHNNDTCEPIKSGFGYXXXXXXXXXXXXXAFHYDRDHFIASIETNQENNFWFXXXXXXXXXXXXXFVIGSVLITKHVXXXXXXXXXXXXXXNIYEEINQAPEDSMYSNMVENQRNVIRSLANYQFHLRSNPNRDTAVYSEIDIAQ 1594 MRHIHLIMATLVFNQFVDIF+GFLIGHLKENFRNKMEIDIFIYFKPISNRRLLLAIDKNNNSFTVLTSKYFHKDFQLIIDFNGLNYSVTSQSAIDLVFDEEIFKINLQLSIGNNNVYELGSTRILLENIFNHTNPTSFDDLLNAYNFHQMIDKTIIFVGNIDNIQLNGAINILFEEIIGC IQAGDYFYPYCSKRATESICEERNCRNRSIDYYTLCCSLSGINAGEHCYKIRTEQQKSSYRISAEDSNIRIAKFAWRPKLLENECLIQIVTKNNSLITIYKNPLGEILLVQQNRQSEIWYKKSLYKLQSNRTHILIVKFVDSI IIKISSGDFIKKFYFPLDASAGYVNVGWTHNNDTCEPIKSGF AFHYDRDHFIASIETNQENNFWFIISISLLSNLLLIFVIGSVLITKHVTKTKGKISTSISKINIYEEINQAPEDSMYSNMVENQRNVIRSLANYQFHLRSNPNRDTAVYSEIDIAQ Sbjct: 1 MRHIHLIMATLVFNQFVDIFRGFLIGHLKENFRNKMEIDIFIYFKPISNRRLLLAIDKNNNSFTVLTSKYFHKDFQLIIDFNGLNYSVTSQSAIDLVFDEEIFKINLQLSIGNNNVYELGSTRILLENIFNHTNPTSFDDLLNAYNFHQMIDKTIIFVGNIDNIQLNGAINILFEEIIGC-------------IQAGDYFYPYCSKRATESICEERNCRNRSIDYYTLCCSLSGINAGEHCYKIRTEQQKSSYRISAEDSNIRIAKFAWRPKLLENECLIQIVTKNNSLITIYKNPLGEILLVQQNRQSEIWYKKSLYKLQSNRTHILIVKFVDSIYIIKISSGDFIKKFYFPLDASAGYVNVGWTHNNDTCEPIKSGF---------------AFHYDRDHFIASIETNQENNFWFIISISLLSNLLLIFVIGSVLITKHVTKTKGKISTSISKINIYEEINQAPEDSMYSNMVENQRNVIRSLANYQFHLRSNPNRDTAVYSEIDIAQ 482
BLAST of SMED30016022 vs. Planmine SMEST
Match: SMESG000053405.1 (SMESG000053405.1) HSP 1 Score: 840.877 bits (2171), Expect = 0.000e+0 Identity = 473/503 (94.04%), Postives = 474/503 (94.23%), Query Frame = 2 Query: 86 MATLVFNQFVDIFQGFLIGHLKENFRNKMEIDIFIYFKPISNRRLLLAIDKNNNSFTVLTSKYFHKDFQLIIDFNGLNYSVTSQSAIDLVFDEEIFKINLQLSIGNNNVYELGSTRILLENIFNHTNPTSFDDLLNAYNFHQMIDKTIIFVGNIDNIQLNGAINILFEEIIGCIQGFYVNEYKTKFIQAGDYFYPYCSKRATESICEERNCRNRSIDYYTLCCSLSGINAGEHCYKIRTEQQKSSYRISAEDSNIRIAKFAWRPKLLENECLIQIVTKNNSLITIYKNPLGEILLVQQNRQSEIWYKKSLYKLQSNRTHILXXXXXXXXXXXXXXXXXXXXXFYFPLDASAGYVNVGWTHNNDTCEPIKSGFGYXXXXXXXXXXXXXAFHYDRDHFIASIETNQENNFWFXXXXXXXXXXXXXFVIGSVLITKHVXXXXXXXXXXXXXXNIYEEINQAPEDSMYSNMVENQRNVIRSLANYQFHLRSNPNRDTAVYSEIDIAQ 1594 MATLVFNQFVDIF+GFLIGHLKENFRNKMEIDIFIYFKPISNRRLLLAIDKNNNSFTVLTSKYFHKDFQLIIDFNGLNYSVTSQSAIDLVFDEEIFKINLQLSIGNNNVYELGSTRILLENIFNHTNPTSFDDLLNAYNFHQMIDKTIIFVGNIDNIQLNGAINILFEEIIGC IQAGDYFYPYCSKRATESICEERNCRNRSIDYYTLCCSLSGINAGEHCYKIRTEQQKSSYRISAEDSNIRIAKFAWRPKLLENECLIQIVTKNNSLITIYKNPLGEILLVQQNRQSEIWYKKSLYKLQSNRTHILIVKFVDSI IIKISSGDFIKKFYFPLDASAGYVNVGWTHNNDTCEPIKSGF AFHYDRDHFIASIETNQENNFWFIISISLLSNLLLIFVIGSVLITKHVTKTKGKISTSISKINIYEEINQAPEDSMYSNMVENQRNVIRSLANYQFHLRSNPNRDTAVYSEIDIAQ Sbjct: 1 MATLVFNQFVDIFRGFLIGHLKENFRNKMEIDIFIYFKPISNRRLLLAIDKNNNSFTVLTSKYFHKDFQLIIDFNGLNYSVTSQSAIDLVFDEEIFKINLQLSIGNNNVYELGSTRILLENIFNHTNPTSFDDLLNAYNFHQMIDKTIIFVGNIDNIQLNGAINILFEEIIGC-------------IQAGDYFYPYCSKRATESICEERNCRNRSIDYYTLCCSLSGINAGEHCYKIRTEQQKSSYRISAEDSNIRIAKFAWRPKLLENECLIQIVTKNNSLITIYKNPLGEILLVQQNRQSEIWYKKSLYKLQSNRTHILIVKFVDSIYIIKISSGDFIKKFYFPLDASAGYVNVGWTHNNDTCEPIKSGF---------------AFHYDRDHFIASIETNQENNFWFIISISLLSNLLLIFVIGSVLITKHVTKTKGKISTSISKINIYEEINQAPEDSMYSNMVENQRNVIRSLANYQFHLRSNPNRDTAVYSEIDIAQ 475
BLAST of SMED30016022 vs. Planmine SMEST
Match: SMESG000056437.1 (SMESG000056437.1) HSP 1 Score: 139.043 bits (349), Expect = 1.425e-35 Identity = 99/326 (30.37%), Postives = 164/326 (50.31%), Query Frame = 2 Query: 101 FNQFVDIFQ-GFLIGHLKENFRNKMEIDIFIYFKPISNRRLLLAIDKNNNSFTVLTSKYFHKDFQLIIDFNGLNYSVTSQSAIDLVFDEEIFKINLQLSIGNNNVYELGSTRILLENIFNH--TNPTSFDDLLNAYNFHQMIDKTIIFVGNIDNIQLNGAINILFE----EIIGCIQGFYVNEYKTKFIQAGDYFYPYCSKRATESICEERNCRNRSIDYYTLCCSLSGINAGEHCYK--IRTEQQKSSYRISAEDSNIRIAKFAWRPKLLENECLIQIVTKNNSLITIYKNPLGEILL-VQQNRQSEIWYKKSLYKLQSNRTHIL 1048 F ++ +F G+L G L ENF N M+ I + F+ +S+ LL AID+N N F + +++ + +++ +FNG ++ T+ F I LQ N + + L N+FN TNP +F + + + T ++GN+ G +F+ +++GC+ F +N + KF GD P+C K+ +E+IC + C+ Y L CSLS +N E+CY+ + Q SY RI K W+ + ++ CL++I TK S I +Y N L EI+L ++ NR E W +++L K+Q N H+L Sbjct: 14 FINYIQLFSSGYLYGKLDENFENGMQFYIDLDFRDVSHGILLYAIDRNRNRFLIESAEKYRGRRKIVFNFNGQIFNSTNTYW--RFSGTSTFTIFLQFKYDNLYIMDYSYRHATLLNVFNRNKTNPDTFVGYPISDDHNSTTRTTDFWLGNVG--PSIGKDKTVFQPSKVQMVGCVYNFRINYKEIKFTTYGDDLLPFCRKQFSETICIKGKCKKEQRRSYILDCSLSEMNTKEYCYEDFLTYMGQTLSYLTVGLGEEPRIMKMVWKQEFSKDYCLLEIETKKESSILVYANRLNEIVLNIRSNRSVE-WTRRTLCKMQPNLIHVL 334
BLAST of SMED30016022 vs. Planmine SMEST
Match: SMESG000056437.1 (SMESG000056437.1) HSP 1 Score: 139.043 bits (349), Expect = 1.708e-35 Identity = 99/326 (30.37%), Postives = 164/326 (50.31%), Query Frame = 2 Query: 101 FNQFVDIFQ-GFLIGHLKENFRNKMEIDIFIYFKPISNRRLLLAIDKNNNSFTVLTSKYFHKDFQLIIDFNGLNYSVTSQSAIDLVFDEEIFKINLQLSIGNNNVYELGSTRILLENIFNH--TNPTSFDDLLNAYNFHQMIDKTIIFVGNIDNIQLNGAINILFE----EIIGCIQGFYVNEYKTKFIQAGDYFYPYCSKRATESICEERNCRNRSIDYYTLCCSLSGINAGEHCYK--IRTEQQKSSYRISAEDSNIRIAKFAWRPKLLENECLIQIVTKNNSLITIYKNPLGEILL-VQQNRQSEIWYKKSLYKLQSNRTHIL 1048 F ++ +F G+L G L ENF N M+ I + F+ +S+ LL AID+N N F + +++ + +++ +FNG ++ T+ F I LQ N + + L N+FN TNP +F + + + T ++GN+ G +F+ +++GC+ F +N + KF GD P+C K+ +E+IC + C+ Y L CSLS +N E+CY+ + Q SY RI K W+ + ++ CL++I TK S I +Y N L EI+L ++ NR E W +++L K+Q N H+L Sbjct: 13 FINYIQLFSSGYLYGKLDENFENGMQFYIDLDFRDVSHGILLYAIDRNRNRFLIESAEKYRGRRKIVFNFNGQIFNSTNTYW--RFSGTSTFTIFLQFKYDNLYIMDYSYRHATLLNVFNRNKTNPDTFVGYPISDDHNSTTRTTDFWLGNVG--PSIGKDKTVFQPSKVQMVGCVYNFRINYKEIKFTTYGDDLLPFCRKQFSETICIKGKCKKEQRRSYILDCSLSEMNTKEYCYEDFLTYMGQTLSYLTVGLGEEPRIMKMVWKQEFSKDYCLLEIETKKESSILVYANRLNEIVLNIRSNRSVE-WTRRTLCKMQPNLIHVL 333
BLAST of SMED30016022 vs. Planmine SMEST
Match: SMESG000003492.1 (SMESG000003492.1) HSP 1 Score: 139.428 bits (350), Expect = 1.713e-35 Identity = 121/375 (32.27%), Postives = 179/375 (47.73%), Query Frame = 2 Query: 95 LVFNQFVDIFQGFLIGHLKENFRNKMEIDIFIYFKPISNRRLLLAIDKNNNSFTVLTSKYFHKDFQLIIDFNGLNYSVT----SQSAIDLVFDEEIFKINLQLSIGNNNVYELGSTRILLENIFNHTNPTSFDDLLNAYNFHQMIDKTIIFVGNIDNIQLNGAINILFEEIIGCIQGFYVNEYKTKFIQAGDYFYPYCSKRATESICEERNCRNRSIDY-YTLCCSLSGINAGEHCYKIRTEQQKSSYRISAEDSNIRIAKFAWRPKLLENECLIQIVTKNNSLITIYKNPLGEILLVQQNRQSEIWYKKSLYKLQSNRTHILXXXXXXXXXXXXXXXXXXXXXFYFPLDASAGYVNVGWTHNNDTCEPIKSGFG 1204 LV I +LIG ++ FRN + I I I F IS++ LL AID N N ++ TSK F D +LI++FNG + S A DE I SI ++R + EN + P + D M ++ I++G + N +IIGC++ VN F++ G+ FY C R TESICE+ CRN + ++ Y L C L G++ HCY T+ + K AW P+L +CLIQI+T+N+S+I IY N +I++ + +S K + Y++ S H L + F+D I+IS+G FIK P +N+GW N C+PI+ FG Sbjct: 22 LVLVSIFGISSSYLIGEIEGEFRNDVTIGIRIGFSRISDKYLLHAIDANQNKLSIKTSKSFASDSKLILNFNGATRAFKCLENSLYAAKWPSDELIISNISYSSI---------NSRSVTENDW----PAAGD----------MSNRMKIWLGYDHDWISKSHSNWPTNQIIGCVELIDVNGKSIIFVKHGNDFYMDCYNRVTESICEKSQCRNETDNHQYILNCRLPGLSTSPHCYPDITDNTGGLHSYQPITHGHVAVKIAWIPQLSIRQCLIQIITRNDSIIDIYINSANQIMIEITDNKSIKSSKYNSYQISSTDVHALSMGFIDHKLTIRISNGYFIKTRMLPNHFRVETINIGWNSNPYLCQPIRGKFG 373 The following BLAST results are available for this feature:
BLAST of SMED30016022 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30016022 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30016022 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30016022 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30016022 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30016022 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30016022 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30016022 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30016022 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30016022 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30016022 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30016022 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30016022 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30016022 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30016022 ID=SMED30016022|Name=SMED30016022|organism=Schmidtea mediterranea sexual|type=transcript|length=1611bpback to top protein sequence of SMED30016022-orf-1 >SMED30016022-orf-1 ID=SMED30016022-orf-1|Name=SMED30016022-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=511bp MRHIHLIMATLVFNQFVDIFQGFLIGHLKENFRNKMEIDIFIYFKPISNRback to top Annotated Terms
The following terms have been associated with this transcript:
|