SMED30015991

Overview
NameSMED30015991
Smed IDSMED30015991
Length (bp)1342
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30015991 (SMED30015991) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30015991 (SMED30015991) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30015991 (SMED30015991) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30015991 (SMED30015991) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30015991

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30015991 dd_Smed_v4_13448_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30015991 dd_Smed_v4_13448_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30015991 dd_Smed_v4_13448_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30015991 vs. Ensembl Xenopus
Match: ENSXETT00000011934.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:3:51966053:51967075:1 gene:ENSXETG00000005538.1 transcript:ENSXETT00000011934.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 40.4318 bits (93), Expect = 6.804e-7
Identity = 20/38 (52.63%), Postives = 27/38 (71.05%), Query Frame = -2 
Query:  466 VFPDFKKEFILHTDASFDTIGAVLSQRDEQGHKHVIAY 579
            + P+FKKEF++ TDAS   +GAVLSQ    G +H +AY
Sbjct:  100 IAPNFKKEFLVQTDASEVGLGAVLSQ-VVNGEEHPVAY 136

HSP 2 Score: 33.113 bits (74), Expect = 6.804e-7
Identity = 20/63 (31.75%), Postives = 33/63 (52.38%), Query Frame = -1 
Query:  290 LSHAMSTHERVYCITRKELLAIFFS-RHFNYYLYCKRFVLRTDHKAISFMLTSKKP---ITTW 466
            LS  ++  E  Y I  +E LAI ++     YYL  ++F L TDH  + +M  +++    +T W
Sbjct:  137 LSRKLTPAECRYAIVERECLAIKWALESLRYYLLGRQFKLITDHAPLKWMAQNREKNARVTRW 199
[Close]
BLAST of SMED30015991 vs. Ensembl Xenopus
Match: ENSXETT00000024810.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV464121.1:1437:3201:1 gene:ENSXETG00000014958.1 transcript:ENSXETT00000024810.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 49.2914 bits (116), Expect = 1.567e-6
Identity = 26/55 (47.27%), Postives = 33/55 (60.00%), Query Frame = -2 
Query:  409 PDFKKEFILHTDASFDTIGAVLSQRDEQGHKHVIAYYHMP*VPTKEYIVSHEKNS 573
            PDF + FI+HTDAS   IGAVLSQ DE+G +H I Y     +P +    + EK  
Sbjct:  178 PDFSRGFIVHTDASTYGIGAVLSQVDEKGGEHPIIYLSRKLLPREVAYATIEKEC 232

HSP 2 Score: 23.0978 bits (48), Expect = 1.567e-6
Identity = 8/23 (34.78%), Postives = 11/23 (47.83%), Query Frame = -3 
Query:  582 IIWTE*CEKDFEEMKIALTTAPI 650
            + WT  C      +K AL  AP+
Sbjct:  152 VTWTPECATAMSALKSALVNAPV 174
[Close]
BLAST of SMED30015991 vs. UniProt/SwissProt
Match: sp|P10394|POL4_DROME (Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaster OX=7227 GN=POL PE=4 SV=1)

HSP 1 Score: 38.5058 bits (88), Expect = 1.169e-6
Identity = 19/37 (51.35%), Postives = 25/37 (67.57%), Query Frame = -2 
Query:  466 FPDFKKEFILHTDASFDTIGAVLSQRDEQGHKHVIAY 576
            +PDF KEF + TDAS    GAVL+Q +  GH+  +AY
Sbjct:  609 YPDFSKEFCITTDASKQACGAVLTQ-NHNGHQLPVAY 644

HSP 2 Score: 36.1946 bits (82), Expect = 1.169e-6
Identity = 22/67 (32.84%), Postives = 36/67 (53.73%), Query Frame = -1 
Query:  266 SHAMSTHERVYCITRKELLAIFFSR-HFNYYLYCKRFVLRTDHKAISFMLTSKKPITTWNSEKVQRI 463
            S A +  E     T +EL AI ++  HF  Y+Y K F ++TDH+ ++++ +   P     S K+ RI
Sbjct:  646 SRAFTKGESNKSTTEQELAAIHWAIIHFRPYIYGKHFTVKTDHRPLTYLFSMVNP-----SSKLTRI 707
[Close]
BLAST of SMED30015991 vs. UniProt/SwissProt
Match: sp|P20825|POL2_DROME (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 38.891 bits (89), Expect = 1.359e-6
Identity = 18/56 (32.14%), Postives = 35/56 (62.50%), Query Frame = -1 
Query:  302 LSHAMSTHERVYCITRKELLAIFF-SRHFNYYLYCKRFVLRTDHKAISFMLTSKKP 466
            +S  ++ HE  Y    KELLAI + ++ F +YL  ++F++ +DH+ + ++   K+P
Sbjct:  533 ISRTLNDHELNYSAIEKELLAIVWATKTFRHYLLGRQFLIASDHQPLRWLHNLKEP 588

HSP 2 Score: 35.4242 bits (80), Expect = 1.359e-6
Identity = 18/31 (58.06%), Postives = 22/31 (70.97%), Query Frame = -2 
Query:  484 FPDFKKEFILHTDASFDTIGAVLSQRDEQGH 576
             PDF+K+F+L TDAS   +GAVLSQ    GH
Sbjct:  501 LPDFEKKFVLTTDASNLALGAVLSQ---NGH 528
[Close]
BLAST of SMED30015991 vs. TrEMBL
Match: A0A087T293 (Retrovirus-related Pol polyprotein from transposon 412 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_23274 PE=4 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 8.793e-18
Identity = 32/70 (45.71%), Postives = 47/70 (67.14%), Query Frame = -1 
Query:  266 SHAMSTHERVYCITRKELLAIFFS-RHFNYYLYCKRFVLRTDHKAISFMLTSKKP---ITTWNSEKVQRI 463
            S  +S  ER YC+TRKELLAI  +  HF++YLY ++F+LRTDH +++++L  K P   I  W    +QR+
Sbjct: 1026 SKCLSKPERNYCVTRKELLAIVKAIEHFHHYLYGRKFLLRTDHASLTWLLNFKNPEGQIARW----IQRL 1091

HSP 2 Score: 41.2022 bits (95), Expect = 8.793e-18
Identity = 20/38 (52.63%), Postives = 28/38 (73.68%), Query Frame = -2 
Query:  463 FPDFKKEFILHTDASFDTIGAVLSQRDEQGHKHVIAYY 576
            +P   K+FIL TDAS +++GAVLSQ  + G + VIAY+
Sbjct:  989 YPQIDKQFILDTDASNESVGAVLSQMID-GEERVIAYW 1025

HSP 3 Score: 35.4242 bits (80), Expect = 8.793e-18
Identity = 13/24 (54.17%), Postives = 18/24 (75.00%), Query Frame = -3 
Query:  582 QIIWTE*CEKDFEEMKIALTTAPI 653
            + +WTE CEK F E+K ALT+ P+
Sbjct:  963 KFVWTEECEKSFNELKTALTSTPV 986
[Close]
BLAST of SMED30015991 vs. TrEMBL
Match: A0A498N0Y8 (Transposon Ty3-G Gag-Pol poly OS=Labeo rohita OX=84645 GN=ROHU_019690 PE=4 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 3.318e-17
Identity = 27/55 (49.09%), Postives = 39/55 (70.91%), Query Frame = -1 
Query:  302 SHAMSTHERVYCITRKELLAIFFS-RHFNYYLYCKRFVLRTDHKAISFMLTSKKP 463
            S ++S  ER YC+TR+ELLA+  + RHF  YL   RF LRTDH ++++ML  ++P
Sbjct:  565 SCSLSRPERNYCVTRRELLAVILAVRHFRPYLLGTRFTLRTDHASLTWMLNFRQP 619

HSP 2 Score: 41.2022 bits (95), Expect = 3.318e-17
Identity = 20/38 (52.63%), Postives = 27/38 (71.05%), Query Frame = -2 
Query:  463 FPDFKKEFILHTDASFDTIGAVLSQRDEQGHKHVIAYY 576
            +PD  + F++ TDAS   +GAVLSQR E G + V+AYY
Sbjct:  528 YPDPNQPFLVDTDASNVGVGAVLSQRGETGER-VVAYY 564

HSP 3 Score: 28.8758 bits (63), Expect = 3.318e-17
Identity = 10/24 (41.67%), Postives = 15/24 (62.50%), Query Frame = -3 
Query:  582 QIIWTE*CEKDFEEMKIALTTAPI 653
            Q  W+E C   F+++K AL  AP+
Sbjct:  502 QFGWSEDCAAAFQQLKAALIDAPV 525

HSP 4 Score: 26.5646 bits (57), Expect = 3.318e-17
Identity = 14/44 (31.82%), Postives = 22/44 (50.00%), Query Frame = -3 
Query:  201 YANVKEAYN-NMEFRKGTENINADLLDRNTCLT--CVQCLMDHE 323
            +  + + Y+  ++ R G ++ NAD L R  CLT  C  C    E
Sbjct:  626 WLEILQEYDFEVQHRPGRQHANADALSRRPCLTDECRYCSRQEE 669
[Close]
BLAST of SMED30015991 vs. TrEMBL
Match: A0A087T3I4 (Retrovirus-related Pol polyprotein from transposon 412 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_13332 PE=4 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 3.540e-17
Identity = 32/70 (45.71%), Postives = 47/70 (67.14%), Query Frame = -1 
Query:  266 SHAMSTHERVYCITRKELLAIFFS-RHFNYYLYCKRFVLRTDHKAISFMLTSKKP---ITTWNSEKVQRI 463
            S  +S  ER YC+TRKELLAI  +  HF++YLY ++F+LRTDH +++++L  K P   I  W    +QR+
Sbjct: 1010 SKCLSKPERNYCVTRKELLAIVKAVEHFHHYLYGRKFLLRTDHASLAWLLNFKNPEGQIARW----IQRL 1075

HSP 2 Score: 40.817 bits (94), Expect = 3.540e-17
Identity = 19/38 (50.00%), Postives = 27/38 (71.05%), Query Frame = -2 
Query:  463 FPDFKKEFILHTDASFDTIGAVLSQRDEQGHKHVIAYY 576
            +P   + FIL TDAS +++GAVLSQ  E G + V+AY+
Sbjct:  973 YPKLDRPFILDTDASNESVGAVLSQEIE-GQERVVAYW 1009

HSP 3 Score: 31.5722 bits (70), Expect = 3.540e-17
Identity = 12/24 (50.00%), Postives = 18/24 (75.00%), Query Frame = -3 
Query:  582 QIIWTE*CEKDFEEMKIALTTAPI 653
            + +WT+ CE  F+ +K ALT+API
Sbjct:  947 KFVWTKECENAFKNLKEALTSAPI 970

HSP 4 Score: 21.557 bits (44), Expect = 3.540e-17
Identity = 9/17 (52.94%), Postives = 10/17 (58.82%), Query Frame = -3 
Query:  234 RKGTENINADLLDRNTC 284
            RKG  + NAD L R  C
Sbjct: 1085 RKGQSHGNADALSRRPC 1101
[Close]
BLAST of SMED30015991 vs. TrEMBL
Match: A0A087SUZ9 (Retrovirus-related Pol polyprotein from transposon 412 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_16630 PE=4 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 7.188e-17
Identity = 32/70 (45.71%), Postives = 47/70 (67.14%), Query Frame = -1 
Query:  266 SHAMSTHERVYCITRKELLAIFFS-RHFNYYLYCKRFVLRTDHKAISFMLTSKKP---ITTWNSEKVQRI 463
            S  +S  ER YC+TRKELLAI  +  HF++YLY ++F+LRTDH +++++L  K P   I  W    +QR+
Sbjct:  996 SKCLSKPERNYCVTRKELLAIVKAVEHFHHYLYGRKFLLRTDHASLAWLLNFKNPEGQIARW----IQRL 1061

HSP 2 Score: 40.4318 bits (93), Expect = 7.188e-17
Identity = 19/38 (50.00%), Postives = 27/38 (71.05%), Query Frame = -2 
Query:  463 FPDFKKEFILHTDASFDTIGAVLSQRDEQGHKHVIAYY 576
            +P   + FIL TDAS +++GAVLSQ  E G + V+AY+
Sbjct:  959 YPQLDRPFILDTDASNESVGAVLSQEIE-GQERVVAYW 995

HSP 3 Score: 31.187 bits (69), Expect = 7.188e-17
Identity = 12/24 (50.00%), Postives = 18/24 (75.00%), Query Frame = -3 
Query:  582 QIIWTE*CEKDFEEMKIALTTAPI 653
            + +WT+ CE  F+ +K ALT+API
Sbjct:  933 KFVWTKECENAFKNLKEALTSAPI 956

HSP 4 Score: 21.557 bits (44), Expect = 7.188e-17
Identity = 9/17 (52.94%), Postives = 10/17 (58.82%), Query Frame = -3 
Query:  234 RKGTENINADLLDRNTC 284
            RKG  + NAD L R  C
Sbjct: 1071 RKGQSHGNADALSRRPC 1087
[Close]
BLAST of SMED30015991 vs. TrEMBL
Match: A0A5S6QIC1 (Uncharacterized protein OS=Trichuris muris OX=70415 PE=4 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 1.216e-15
Identity = 23/37 (62.16%), Postives = 29/37 (78.38%), Query Frame = -2 
Query:  466 FPDFKKEFILHTDASFDTIGAVLSQRDEQGHKHVIAY 576
            FPDF + FIL TDAS   IGAVLSQ D++GH+H +A+
Sbjct:  889 FPDFTRPFILDTDASTTAIGAVLSQLDQEGHEHPVAF 925

HSP 2 Score: 53.1434 bits (126), Expect = 1.216e-15
Identity = 29/68 (42.65%), Postives = 42/68 (61.76%), Query Frame = -1 
Query:  269 SHAMSTHERVYCITRKELLAIF-FSRHFNYYLYCKRFVLRTDHKAISFMLTSKKPITTWN--SEKVQR 463
            S  +S  E+ YC+TR+E+LA+  F +HF  YL    FVLRTDH ++ ++ + K P   W    EK+QR
Sbjct:  927 SRILSRAEQKYCVTRREMLAVVTFVQHFAPYLQ-DEFVLRTDHGSLQWLQSFKDPDGQWARWQEKLQR 993

HSP 3 Score: 26.5646 bits (57), Expect = 1.216e-15
Identity = 11/25 (44.00%), Postives = 16/25 (64.00%), Query Frame = -3 
Query:  573 IWTE*CEKDFEEMKIALTTAPIPCF 647
            +W+  CE+ F  +K ALT+ PI  F
Sbjct:  865 VWSRDCEQAFLTLKKALTSYPILSF 889
[Close]
BLAST of SMED30015991 vs. Ensembl Cavefish
Match: ENSAMXT00000048272.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000106.1:219951:223307:1 gene:ENSAMXG00000032559.1 transcript:ENSAMXT00000048272.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.8398 bits (133), Expect = 7.383e-14
Identity = 23/55 (41.82%), Postives = 39/55 (70.91%), Query Frame = -1 
Query:  302 SHAMSTHERVYCITRKELLAIF-FSRHFNYYLYCKRFVLRTDHKAISFMLTSKKP 463
            S  +S+ E+ YC TR+ELLA+  F+RHF  YL  + F++R+DH ++ +++  K+P
Sbjct:  404 SRRLSSTEQNYCTTRRELLAVVEFTRHFRQYLLGRPFIVRSDHSSLRWLVNMKEP 458

HSP 2 Score: 28.8758 bits (63), Expect = 7.383e-14
Identity = 18/37 (48.65%), Postives = 22/37 (59.46%), Query Frame = -2 
Query:  466 FPDFKKEFILHTDASFDTIGAVLSQRDEQGHKHVIAY 576
            +P      IL TDAS   IGAVLSQ  + G + V+AY
Sbjct:  367 YPRDHGNLILDTDASNFGIGAVLSQVQD-GAERVLAY 402

HSP 3 Score: 28.1054 bits (61), Expect = 7.383e-14
Identity = 10/21 (47.62%), Postives = 15/21 (71.43%), Query Frame = -3 
Query:  582 WTE*CEKDFEEMKIALTTAPI 644
            WT  C+  FEE+K +LT+ P+
Sbjct:  344 WTPECQTAFEELKSSLTSTPV 364

HSP 4 Score: 21.9422 bits (45), Expect = 7.383e-14
Identity = 10/25 (40.00%), Postives = 13/25 (52.00%), Query Frame = -3 
Query:  210 RKGTENINADLLDRNTCLTCVQCLM 284
            R G  + NAD++ R  C T   C M
Sbjct:  479 RPGHHHQNADVMSRRPCRTTCPCNM 503
[Close]
BLAST of SMED30015991 vs. Ensembl Cavefish
Match: ENSAMXT00000052614.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001686.1:18497:23810:-1 gene:ENSAMXG00000038033.1 transcript:ENSAMXT00000052614.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 54.299 bits (129), Expect = 1.890e-13
Identity = 23/55 (41.82%), Postives = 39/55 (70.91%), Query Frame = -1 
Query:  302 SHAMSTHERVYCITRKELLAIF-FSRHFNYYLYCKRFVLRTDHKAISFMLTSKKP 463
            S  +S+ E+ YC TR+ELLA+  F+RHF  YL  + F++R+DH ++ +++  K+P
Sbjct:  229 SRRLSSTEQNYCTTRRELLAVVEFTRHFRQYLLGRPFIVRSDHSSLRWLVNIKEP 283

HSP 2 Score: 28.8758 bits (63), Expect = 1.890e-13
Identity = 18/37 (48.65%), Postives = 22/37 (59.46%), Query Frame = -2 
Query:  466 FPDFKKEFILHTDASFDTIGAVLSQRDEQGHKHVIAY 576
            +P      IL TDAS   IGAVLSQ  + G + V+AY
Sbjct:  192 YPRDHGNLILDTDASNFGIGAVLSQVQD-GAERVLAY 227

HSP 3 Score: 28.4906 bits (62), Expect = 1.890e-13
Identity = 10/21 (47.62%), Postives = 15/21 (71.43%), Query Frame = -3 
Query:  582 WTE*CEKDFEEMKIALTTAPI 644
            WT  C+  FEE+K +LT+ P+
Sbjct:  169 WTPECQAAFEELKSSLTSTPV 189

HSP 4 Score: 21.9422 bits (45), Expect = 1.890e-13
Identity = 10/25 (40.00%), Postives = 13/25 (52.00%), Query Frame = -3 
Query:  210 RKGTENINADLLDRNTCLTCVQCLM 284
            R G  + NAD++ R  C T   C M
Sbjct:  304 RPGHHHQNADVMSRRPCRTTCPCNM 328
[Close]
BLAST of SMED30015991 vs. Ensembl Cavefish
Match: ENSAMXT00000039384.1 (pep primary_assembly:Astyanax_mexicanus-2.0:25:21146036:21149703:-1 gene:ENSAMXG00000035834.1 transcript:ENSAMXT00000039384.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.9878 bits (123), Expect = 2.833e-13
Identity = 23/55 (41.82%), Postives = 37/55 (67.27%), Query Frame = -1 
Query:  302 SHAMSTHERVYCITRKELLAIF-FSRHFNYYLYCKRFVLRTDHKAISFMLTSKKP 463
            S A+S  E+ Y  T+KELLA+  F+ +F +YL  +RF+LRTDH ++ ++    +P
Sbjct:  178 SRALSRQEKNYATTKKELLAVVHFTSYFRHYLLGRRFLLRTDHSSLRWLHNFSQP 232

HSP 2 Score: 36.5798 bits (83), Expect = 2.833e-13
Identity = 20/38 (52.63%), Postives = 26/38 (68.42%), Query Frame = -2 
Query:  466 VFPDFKKEFILHTDASFDTIGAVLSQRDEQGHKHVIAY 579
             FPD  + FIL TDAS   IGA+LSQ+ + G + V+AY
Sbjct:  140 TFPDPGQTFILDTDASDVAIGAILSQKID-GFEKVVAY 176

HSP 3 Score: 25.409 bits (54), Expect = 2.833e-13
Identity = 10/24 (41.67%), Postives = 15/24 (62.50%), Query Frame = -3 
Query:  573 WTE*CEKDFEEMKIALTTAPIPCF 644
            WT+ C+  F+ +K  L +API  F
Sbjct:  118 WTQECQTAFQTLKDKLVSAPILTF 141
[Close]
BLAST of SMED30015991 vs. Ensembl Cavefish
Match: ENSAMXT00000038919.1 (pep primary_assembly:Astyanax_mexicanus-2.0:25:7611858:7613942:-1 gene:ENSAMXG00000031762.1 transcript:ENSAMXT00000038919.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.447 bits (119), Expect = 1.563e-12
Identity = 23/55 (41.82%), Postives = 36/55 (65.45%), Query Frame = -1 
Query:  302 SHAMSTHERVYCITRKELLAIF-FSRHFNYYLYCKRFVLRTDHKAISFMLTSKKP 463
            S  +S  E+ YC TR+ELLA+  F+ HF  YL  + F +RTDH ++ +++  K+P
Sbjct:  165 SRRLSQTEQNYCTTRRELLAVVEFTSHFRQYLLGRTFTVRTDHSSLRWLMRMKEP 219

HSP 2 Score: 29.6462 bits (65), Expect = 1.563e-12
Identity = 12/30 (40.00%), Postives = 19/30 (63.33%), Query Frame = -3 
Query:  555 WTE*CEKDFEEMKIALTTAPIPCFRISRKN 644
            WT  C++ FEE+K  LT+AP+  + +   N
Sbjct:  105 WTAECQEAFEELKRRLTSAPVLGYPLDTGN 134

HSP 3 Score: 25.7942 bits (55), Expect = 1.563e-12
Identity = 17/37 (45.95%), Postives = 21/37 (56.76%), Query Frame = -2 
Query:  466 FPDFKKEFILHTDASFDTIGAVLSQRDEQGHKHVIAY 576
            +P      IL TDAS   IGAVLSQ  +   + V+AY
Sbjct:  128 YPLDTGNIILDTDASNFGIGAVLSQ-VQNDEERVLAY 163

HSP 4 Score: 24.2534 bits (51), Expect = 1.563e-12
Identity = 10/40 (25.00%), Postives = 18/40 (45.00%), Query Frame = -3 
Query:  165 RKGTENINADLLDRNTCLTCVQCLMDHENPKSGRLKTRLL 284
            R G ++ NAD + R  C     C++   +  S R   + +
Sbjct:  240 RPGAQHCNADAMSRRPCRKSCPCMVPDPSSSSERFSDQAV 279
[Close]
BLAST of SMED30015991 vs. Ensembl Cavefish
Match: ENSAMXT00000039316.1 (pep primary_assembly:Astyanax_mexicanus-2.0:15:30933509:30934681:1 gene:ENSAMXG00000029182.1 transcript:ENSAMXT00000039316.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.521 bits (114), Expect = 2.237e-12
Identity = 23/55 (41.82%), Postives = 37/55 (67.27%), Query Frame = -1 
Query:  302 SHAMSTHERVYCITRKELLAIF-FSRHFNYYLYCKRFVLRTDHKAISFMLTSKKP 463
            S A+S  E+ Y  T+KELLA+  F+ +F +YL  +RF+LRTDH ++ ++    +P
Sbjct:  190 SRALSRQEKNYATTKKELLAVVHFTSYFRHYLLGRRFLLRTDHSSLRWLHNFSQP 244

HSP 2 Score: 36.1946 bits (82), Expect = 2.237e-12
Identity = 20/38 (52.63%), Postives = 26/38 (68.42%), Query Frame = -2 
Query:  466 VFPDFKKEFILHTDASFDTIGAVLSQRDEQGHKHVIAY 579
             FPD  + FIL TDAS   IGA+LSQ+ + G + V+AY
Sbjct:  152 TFPDPGQTFILDTDASDVAIGAILSQKID-GFEKVVAY 188

HSP 3 Score: 26.1794 bits (56), Expect = 2.237e-12
Identity = 10/24 (41.67%), Postives = 15/24 (62.50%), Query Frame = -3 
Query:  573 WTE*CEKDFEEMKIALTTAPIPCF 644
            WT+ C+  F+ +K  L +API  F
Sbjct:  130 WTQECQTAFQTLKDKLVSAPILTF 153
[Close]
BLAST of SMED30015991 vs. Ensembl Medaka
Match: ENSORLT00000036827.1 (pep primary_assembly:ASM223467v1:16:28613823:28617539:1 gene:ENSORLG00000023550.1 transcript:ENSORLT00000036827.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.3806 bits (137), Expect = 9.427e-16
Identity = 29/66 (43.94%), Postives = 40/66 (60.61%), Query Frame = -1 
Query:  281 LSHAMSTHERVYCITRKELLAIFFS-RHFNYYLYCKRFVLRTDHKAISFMLTSKKP---ITTWNSE 466
             S  +S  ER YC+TR+ELLA+  +  HF YYL    F +RTDH A+ +++T K+P   I  W  E
Sbjct:  598 FSKTLSKAERRYCVTRRELLAVVKAIGHFRYYLCGLPFTVRTDHSALQWLMTFKEPEGQIARWLEE 663

HSP 2 Score: 35.4242 bits (80), Expect = 9.427e-16
Identity = 20/37 (54.05%), Postives = 24/37 (64.86%), Query Frame = -2 
Query:  463 PDFKKEFILHTDASFDTIGAVLSQRDEQGHKHVIAYY 573
            PD K  FIL TDAS   +GAVLSQ    G + V+AY+
Sbjct:  563 PDPKLPFILDTDASDVGMGAVLSQMGSAGER-VVAYF 598

HSP 3 Score: 29.6462 bits (65), Expect = 9.427e-16
Identity = 11/24 (45.83%), Postives = 16/24 (66.67%), Query Frame = -3 
Query:  582 QIIWTE*CEKDFEEMKIALTTAPI 653
              +WT+ CE+ F  +K ALT +PI
Sbjct:  536 DFVWTQECEQAFSSLKKALTNSPI 559
[Close]
BLAST of SMED30015991 vs. Ensembl Medaka
Match: ENSORLT00000040305.1 (pep primary_assembly:ASM223467v1:1:25459511:25466649:-1 gene:ENSORLG00000028409.1 transcript:ENSORLT00000040305.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.4546 bits (132), Expect = 2.211e-13
Identity = 25/55 (45.45%), Postives = 37/55 (67.27%), Query Frame = -1 
Query:  302 SHAMSTHERVYCITRKELLAIF-FSRHFNYYLYCKRFVLRTDHKAISFMLTSKKP 463
            S  ++T E+ YC TR+ELLA+  F+ HF  YL  + FVLRTDH ++ ++   K+P
Sbjct: 1158 SRRLTTTEQNYCTTRRELLAVVEFTSHFRQYLLGRSFVLRTDHSSLRWLTRLKEP 1212

HSP 2 Score: 30.0314 bits (66), Expect = 2.211e-13
Identity = 12/21 (57.14%), Postives = 16/21 (76.19%), Query Frame = -3 
Query:  582 WTE*CEKDFEEMKIALTTAPI 644
            WT  C++ FEE+K  LT+API
Sbjct: 1098 WTTECQEAFEELKERLTSAPI 1118

HSP 3 Score: 28.4906 bits (62), Expect = 2.211e-13
Identity = 17/37 (45.95%), Postives = 23/37 (62.16%), Query Frame = -2 
Query:  466 FPDFKKEFILHTDASFDTIGAVLSQRDEQGHKHVIAY 576
            +P    E +L TDAS   +GAVLSQ  + G + V+AY
Sbjct: 1121 YPLDSGELLLDTDASDWGVGAVLSQV-QGGEERVLAY 1156
[Close]
BLAST of SMED30015991 vs. Ensembl Medaka
Match: ENSORLT00000033471.1 (pep primary_assembly:ASM223467v1:20:15234491:15238537:-1 gene:ENSORLG00000027117.1 transcript:ENSORLT00000033471.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 39.2762 bits (90), Expect = 4.554e-9
Identity = 20/55 (36.36%), Postives = 36/55 (65.45%), Query Frame = -1 
Query:  305 SHAMSTHERVYCITRKELLAIFFS--RHFNYYLYCKRFVLRTDHKAISFMLTSKK 463
            S  +S+ ER Y + + E LA+ ++    F+ YLY  +FV+RTD+  ++++LT+ K
Sbjct:  569 SRKLSSAERNYPVHQLEFLALKWAVVDKFHDYLYGAQFVVRTDNNPLTYVLTTAK 623

HSP 2 Score: 35.8094 bits (81), Expect = 4.554e-9
Identity = 16/34 (47.06%), Postives = 22/34 (64.71%), Query Frame = -2 
Query:  475 FPDFKKEFILHTDASFDTIGAVLSQRDEQGHKHV 576
            F D  K ++LH DASF  +GAVL+Q   +G + V
Sbjct:  532 FADPSKPYVLHVDASFHGLGAVLNQEHPEGLRPV 565

HSP 3 Score: 23.8682 bits (50), Expect = 4.554e-9
Identity = 9/24 (37.50%), Postives = 14/24 (58.33%), Query Frame = -3 
Query:  573 WTE*CEKDFEEMKIALTTAPIPCF 644
            W E C + F+++   LT AP+  F
Sbjct:  509 WDESCTEAFQQILQCLTNAPVLAF 532
[Close]
BLAST of SMED30015991 vs. Ensembl Medaka
Match: ENSORLT00000043530.1 (pep primary_assembly:ASM223467v1:7:30263126:30267112:-1 gene:ENSORLG00000028233.1 transcript:ENSORLT00000043530.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 43.1282 bits (100), Expect = 1.637e-8
Identity = 28/61 (45.90%), Postives = 34/61 (55.74%), Query Frame = -1 
Query:  305 RHCIL--SHAMSTHERVYCITRKELLAIFFS-RHFNYYLYCKRFVLRTDHKAISFMLTSKK 478
            RH I   S A+S  E  Y  T KE LAI +S  HF+ YLY   F L TDHKA+  +  + K
Sbjct:  768 RHVIAYASRALSDVETRYSQTEKEALAIVWSCEHFHLYLYGHPFTLVTDHKALEAIWNNPK 828

HSP 2 Score: 35.039 bits (79), Expect = 1.637e-8
Identity = 17/32 (53.12%), Postives = 21/32 (65.62%), Query Frame = -2 
Query:  466 KEFILHTDASFDTIGAVLSQRDEQGHKHVIAY 561
            +E  L  DAS   +GA+L QRDE   +HVIAY
Sbjct:  742 RETELLVDASPVGLGAILCQRDETAERHVIAY 773
[Close]
BLAST of SMED30015991 vs. Ensembl Medaka
Match: ENSORLT00000040051.1 (pep primary_assembly:ASM223467v1:1:37354481:37359289:-1 gene:ENSORLG00000026212.1 transcript:ENSORLT00000040051.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 34.2686 bits (77), Expect = 3.023e-8
Identity = 19/38 (50.00%), Postives = 24/38 (63.16%), Query Frame = -2 
Query:  466 VFPDFKKEFILHTDASFDTIGAVLSQRDEQGHKHVIAY 579
            V PD    F +HTDAS   +GAVL+Q   +G + VIAY
Sbjct: 1020 VQPDLSLPFEVHTDASDLGLGAVLAQMTSEGER-VIAY 1056

HSP 2 Score: 31.9574 bits (71), Expect = 3.023e-8
Identity = 20/69 (28.99%), Postives = 35/69 (50.72%), Query Frame = -1 
Query:  269 SHAMSTHERVYCITRKELLAIFFS-RHFNYYLYCKRFVLRTDHKAISFMLTSKKP---ITTWNSEKVQR 463
            S  +   ER Y  + KE LA+ ++   + +YL    F + TDH A+++     K    +T W + ++QR
Sbjct: 1058 SRGLKGAERNYSTSEKECLAVVWAVEKWQHYLEGAEFTVFTDHAALTWAFNCPKTSSRLTRW-TLRLQR 1125

HSP 3 Score: 30.4166 bits (67), Expect = 3.023e-8
Identity = 11/21 (52.38%), Postives = 15/21 (71.43%), Query Frame = -3 
Query:  582 WTE*CEKDFEEMKIALTTAPI 644
            WTE C++ F ++K AL  API
Sbjct:  998 WTEECQRSFNQLKQALAKAPI 1018
[Close]
BLAST of SMED30015991 vs. Planmine SMEST
Match: SMESG000029259.1 (SMESG000029259.1)

HSP 1 Score: 99.3673 bits (246), Expect = 4.385e-37
Identity = 47/63 (74.60%), Postives = 55/63 (87.30%), Query Frame = -1 
Query:  290 SHAMSTHERVYCITRKELLAIF-FSRHFNYYLYCKRFVLRTDHKAISFMLTSKKPIT----TW 463
            SHAMS+HE+ YCITRKELLAI+ F +HFN+YLY KRFVLRTDHKAI+FM+T+KKPIT    TW
Sbjct: 3056 SHAMSSHEKGYCITRKELLAIYYFCKHFNHYLYGKRFVLRTDHKAITFMVTTKKPITAQFQTW 3118

HSP 2 Score: 75.0998 bits (183), Expect = 4.385e-37
Identity = 33/38 (86.84%), Postives = 37/38 (97.37%), Query Frame = -2 
Query:  466 VFPDFKKEFILHTDASFDTIGAVLSQRDEQGHKHVIAY 579
            VFPDF+KEFIL TDASFDTIGAVLSQ+DE+GH+HVIAY
Sbjct: 3017 VFPDFRKEFILDTDASFDTIGAVLSQKDEKGHEHVIAY 3054

HSP 3 Score: 98.9821 bits (245), Expect = 1.186e-21
Identity = 73/200 (36.50%), Postives = 101/200 (50.50%), Query Frame = -3 
Query:  108 QIIWTE*CEKDFEEMKIALTTAPIPCFRISRKNSYYIQTQALIQ-----LEQYYHKGMNRDINTSLHIITCHE----YPRKSIL---YHTKRTLGYIFXXX---XXXXSIL*TVCVTH*S*SNIF--YANVKEAYN-NMEFRKGTENINADLLDRNTCLTCVQCLMDHENPKSGRLKTRLLTLMTDNNGEKWQRDNAEIQ 653
            +I WTE CEK F EMK AL TAP+  F   RK  + + T A        L Q   KG    I    H ++ HE      RK +L   Y  K    Y++            +  +  T    +  F  + N   + +  ME+RKGT + NAD+L R TC TCVQC+M+HE+ K+G++KTR+LT+  +    KWQ DN E+Q
Sbjct: 2992 KIRWTEMCEKAFGEMKEALITAPVLVFPDFRK-EFILDTDASFDTIGAVLSQKDEKGHEHVIAYGSHAMSSHEKGYCITRKELLAIYYFCKHFNHYLYGKRFVLRTDHKAITFMVTTKKPITAQFQTWINYLSSLDIKMEYRKGTSHTNADMLSRKTCGTCVQCMMEHEDAKTGKIKTRILTVTAEGGYNKWQNDNMEVQ 3190
[Close]
BLAST of SMED30015991 vs. Planmine SMEST
Match: SMESG000016546.1 (SMESG000016546.1)

HSP 1 Score: 88.5817 bits (218), Expect = 3.397e-28
Identity = 44/63 (69.84%), Postives = 52/63 (82.54%), Query Frame = -1 
Query:  290 SHAMSTHERVYCITRKELLAIF-FSRHFNYYLYCKRFVLRTDHKAISFMLTSKKPIT----TW 463
            SHAMSTHE+ YCI RKELLAI+ F +HFN++LY KRF LRTD KAI+FML++KKPIT    TW
Sbjct:  442 SHAMSTHEKGYCIKRKELLAIYYFCQHFNHHLYGKRFTLRTDLKAITFMLSTKKPITAQFKTW 504

HSP 2 Score: 55.8398 bits (133), Expect = 3.397e-28
Identity = 23/37 (62.16%), Postives = 31/37 (83.78%), Query Frame = -2 
Query:  466 FPDFKKEFILHTDASFDTIGAVLSQRDEQGHKHVIAY 576
            +PDF ++FI+ TD+SFDTIG VLSQ+D  G++ VIAY
Sbjct:  404 YPDFTRDFIIDTDSSFDTIGDVLSQKDNNGYEKVIAY 440
[Close]
BLAST of SMED30015991 vs. Planmine SMEST
Match: SMESG000080555.1 (SMESG000080555.1)

HSP 1 Score: 58.151 bits (139), Expect = 9.448e-19
Identity = 30/53 (56.60%), Postives = 38/53 (71.70%), Query Frame = -1 
Query:  308 SHAMSTHERVYCITRKELLAIF-FSRHFNYYLYCKRFVLRTDHKAISFMLTSK 463
            S  ++ HE  YC+TRKELLA+  F  HF  YLY +RFV  TDHKA++FM T+K
Sbjct:  184 SRHLTKHEMGYCVTRKELLALHEFIVHFRQYLYWRRFVDTTDHKALTFMNTTK 236

HSP 2 Score: 39.6614 bits (91), Expect = 9.448e-19
Identity = 19/30 (63.33%), Postives = 23/30 (76.67%), Query Frame = -2 
Query:  466 FILHTDASFDTIGAVLSQRDEQGHKHVIAY 555
            FI+ TDASF+ IG+VLSQ  E G + VIAY
Sbjct:  153 FIIDTDASFEAIGSVLSQIKEDGTEGVIAY 182

HSP 3 Score: 34.2686 bits (77), Expect = 9.448e-19
Identity = 17/54 (31.48%), Postives = 31/54 (57.41%), Query Frame = -3 
Query:  165 FYANVKEAYNNMEFRKGTENINADLLDRNTCLTCVQCLMDHENPKSGRLKTRLL 326
            + AN+ E    +++RKG E+ NAD + R     C  C M++++ K  + +TR +
Sbjct:  245 WMANLSEYDFALQYRKGEEHGNADGMSRLNNTICSHCQMENKDAKEAKCRTRYI 298
[Close]
BLAST of SMED30015991 vs. Planmine SMEST
Match: SMESG000029320.1 (SMESG000029320.1)

HSP 1 Score: 70.8626 bits (172), Expect = 2.267e-17
Identity = 37/63 (58.73%), Postives = 45/63 (71.43%), Query Frame = -1 
Query:  290 SHAMSTHERVYCITRKELLAIF-FSRHFNYYLYCKRFVLRTDHKAISFMLTSKKPIT----TW 463
            S  ++ HE  YC+TRKELLAI  F  HF  YLY +RFV RTDHKA++FM T+KKPI+    TW
Sbjct:  528 SRHLAKHEMGYCVTRKELLAIHEFIVHFRQYLYGRRFVARTDHKALTFMNTAKKPISPQFQTW 590

HSP 2 Score: 37.3502 bits (85), Expect = 2.267e-17
Identity = 19/30 (63.33%), Postives = 21/30 (70.00%), Query Frame = -2 
Query:  466 FILHTDASFDTIGAVLSQRDEQGHKHVIAY 555
            FI+ TDASF  IGAVLSQ  E   + VIAY
Sbjct:  497 FIIDTDASFGAIGAVLSQIKEDSTEGVIAY 526
[Close]
BLAST of SMED30015991 vs. Planmine SMEST
Match: SMESG000038955.1 (SMESG000038955.1)

HSP 1 Score: 63.929 bits (154), Expect = 2.812e-17
Identity = 34/63 (53.97%), Postives = 44/63 (69.84%), Query Frame = -1 
Query:  290 SHAMSTHERVYCITRKELLAIF-FSRHFNYYLYCKRFVLRTDHKAISFMLTSKKPIT----TW 463
            S  ++ HE  +C+T KELLA+  F  HF  YLY +RFV RTDHKA++FM T+KKPI+    TW
Sbjct:  426 SRHLTKHEMGHCVTIKELLALHEFIVHFRQYLYGRRFVARTDHKALTFMNTTKKPISPQFQTW 488

HSP 2 Score: 37.7354 bits (86), Expect = 2.812e-17
Identity = 19/37 (51.35%), Postives = 24/37 (64.86%), Query Frame = -2 
Query:  466 FPDFKKEFILHTDASFDTIGAVLSQRDEQGHKHVIAY 576
            +P   + F++ TDASF  IGAVLSQ  E   + VIAY
Sbjct:  388 YPRADRLFVIDTDASFGAIGAVLSQIKEDCTEVVIAY 424

HSP 3 Score: 25.409 bits (54), Expect = 2.812e-17
Identity = 11/23 (47.83%), Postives = 13/23 (56.52%), Query Frame = -3 
Query:  582 IIWTE*CEKDFEEMKIALTTAPI 650
            I+WTE C   F  +K  L  API
Sbjct:  363 IVWTEECRNSFINLKKLLCEAPI 385
The following BLAST results are available for this feature:
BLAST of SMED30015991 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015991 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015991 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015991 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015991 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSXETT00000011934.16.804e-752.63pep primary_assembly:Xenopus_tropicalis_v9.1:3:519... [more]
ENSXETT00000024810.11.567e-647.27pep primary_assembly:Xenopus_tropicalis_v9.1:KV464... [more]
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BLAST of SMED30015991 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015991 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|P10394|POL4_DROME1.169e-651.35Retrovirus-related Pol polyprotein from transposon... [more]
sp|P20825|POL2_DROME1.359e-632.14Retrovirus-related Pol polyprotein from transposon... [more]
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BLAST of SMED30015991 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A087T2938.793e-1845.71Retrovirus-related Pol polyprotein from transposon... [more]
A0A498N0Y83.318e-1749.09Transposon Ty3-G Gag-Pol poly OS=Labeo rohita OX=8... [more]
A0A087T3I43.540e-1745.71Retrovirus-related Pol polyprotein from transposon... [more]
A0A087SUZ97.188e-1745.71Retrovirus-related Pol polyprotein from transposon... [more]
A0A5S6QIC11.216e-1562.16Uncharacterized protein OS=Trichuris muris OX=7041... [more]
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BLAST of SMED30015991 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000048272.17.383e-1441.82pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000052614.11.890e-1341.82pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000039384.12.833e-1341.82pep primary_assembly:Astyanax_mexicanus-2.0:25:211... [more]
ENSAMXT00000038919.11.563e-1241.82pep primary_assembly:Astyanax_mexicanus-2.0:25:761... [more]
ENSAMXT00000039316.12.237e-1241.82pep primary_assembly:Astyanax_mexicanus-2.0:15:309... [more]
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BLAST of SMED30015991 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015991 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015991 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015991 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000036827.19.427e-1643.94pep primary_assembly:ASM223467v1:16:28613823:28617... [more]
ENSORLT00000040305.12.211e-1345.45pep primary_assembly:ASM223467v1:1:25459511:254666... [more]
ENSORLT00000033471.14.554e-936.36pep primary_assembly:ASM223467v1:20:15234491:15238... [more]
ENSORLT00000043530.11.637e-845.90pep primary_assembly:ASM223467v1:7:30263126:302671... [more]
ENSORLT00000040051.13.023e-850.00pep primary_assembly:ASM223467v1:1:37354481:373592... [more]
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BLAST of SMED30015991 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000029259.14.385e-3774.60SMESG000029259.1[more]
SMESG000016546.13.397e-2869.84SMESG000016546.1[more]
SMESG000080555.19.448e-1956.60SMESG000080555.1[more]
SMESG000029320.12.267e-1758.73SMESG000029320.1[more]
SMESG000038955.12.812e-1753.97SMESG000038955.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015991 ID=SMED30015991|Name=SMED30015991|organism=Schmidtea mediterranea sexual|type=transcript|length=1342bp
CTCGTCTTTTTGTTGCCTGTATCCATATTTTGTTTTGATTTGTGTAAATA
ATATTTCCTTTTAAAATATATTTATTATTTCTCTTTTGATTAATTTGATT
TTTTTTCTTGTATCTCTGCATTATCTCTTTGCCATTTTTCTCCATTATTA
TCAGTCATCAATGTTAACAATCTAGTTTTCAATCGCCCTGATTTTGGATT
TTCATGGTCCATCAAACATTGTACACATGTTAAGCATGTATTTCTATCAA
GCAAATCTGCGTTTATATTCTCTGTACCTTTTCTGAATTCCATGTTGTTA
TAGGCTTCTTTGACGTTAGCATAAAAGATATTGCTTTATGATCAGTGCGT
AACACAAACCGTTTACAATATAGATAATAGTTGAAGTGACGACTGAAAAA
TATAGCCAAGAGTTCTTTTCGTGTGATACAATATACTCTTTCGTGGGTAC
TCATGGCATGTGATAATATGCAATGACGTGTTTATGTCCCTGTTCATCCC
TTTGTGATAATACTGCTCCAATTGTATCAAAGCTTGCGTCTGTATGTAAT
ATGAATTCTTTCTTGAAATCCGGAAACACGGAATTGGTGCTGTGGTTAAC
GCTATTTTCATTTCTTCGAAGTCTTTCTCACACTACTCAGTCCAGATTAT
TTGGTTTGTATAATAATTACCATTTCTCAGGTGTGAATTTGTTCGAAACT
TCCTTTCTTTGTATCCACGTAAGTGACATCTTTGACATTGAATTGTTGAG
CACTCTGGTCGTATGTGACTTTCCCTCCTCATGCAAAACACATCATTTTC
TTTTTCGTTTGGCATTCTCGACTGATGTTACCAGTTTTCTTACATTTCCA
TCATTCAATTTGTTGGTTATTGACGTTTCGTTGCTTGAAATTAGTATTCA
AGTTTACCAATCTTCTTTGAGCAGCATCTCTGTAGCTTCTGTAGTTTTTA
ACAGCCGGTGAAACCTTCAAAACATTAACATGGCCCATAATCATATTTCA
TTAGTGTTTCATTATATTTTAATGAAATTTGATCAAATCCATCTTGGAAA
TGAGTTTTGCGTTTATTGATAAATATATTTTCATACAGCATTGTTTGTTA
GGGATTTTGTCTTAAACTTTATAATGTGCGACATCTTCTTTAGTCAATCA
AATAAAGTTTTTGATGTCAGAAAAGTGCACAAACGGTTCTCTAGAAATAC
ATCAAACACTAGTATATAATCAAAAGATAAACTAAAAAGAGTTGTCAACA
GGAAGATATCCAAGTTGAGAAAACAAAATTCTAAGTTCGATCGAAGAAGG
AGAGACAAACATGAAATTAATTATTAGTCTTATTATATTTTA
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protein sequence of SMED30015991-orf-1

>SMED30015991-orf-1 ID=SMED30015991-orf-1|Name=SMED30015991-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=163bp
MTCLCPCSSLCDNTAPIVSKLASVCNMNSFLKSGNTELVLWLTLFSFLRS
LSHTTQSRLFGLYNNYHFSGVNLFETSFLCIHVSDIFDIELLSTLVVCDF
PSSCKTHHFLFRLAFSTDVTSFLTFPSFNLLVIDVSLLEISIQVYQSSLS
SISVASVVFNSR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR001878Znf_CCHC
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0008270zinc ion binding