CAP-Gly domain-containing linker protein

Overview
NameCAP-Gly domain-containing linker protein
Smed IDSMED30015988
Length (bp)2241
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of CAP-Gly domain-containing linker protein (SMED30015988) t-SNE clustered cells

Violin plots show distribution of expression levels for CAP-Gly domain-containing linker protein (SMED30015988) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of CAP-Gly domain-containing linker protein (SMED30015988) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for CAP-Gly domain-containing linker protein (SMED30015988) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30015988

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30015988h1SMcG0004754 Contig4561uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30015988h1SMcG0004754 Contig4561newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30015988h1SMcG0006764 Contig4561uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30015988h1SMcG0006764 Contig4561newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
nervous systemSMED30015988h1SMcG0004754 dd_Smed_v4_6540_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30015988h1SMcG0004754 dd_Smed_v4_6540_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30015988h1SMcG0004754 dd_Smed_v4_6540_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30015988h1SMcG0004754 dd_Smed_v4_6540_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30015988h1SMcG0004754 dd_Smed_v4_6540_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Human
Match: CLIP4 (CAP-Gly domain containing linker protein family member 4 [Source:HGNC Symbol;Acc:HGNC:26108])

HSP 1 Score: 191.815 bits (486), Expect = 3.574e-51
Identity = 109/255 (42.75%), Postives = 148/255 (58.04%), Query Frame = 3
Query: 1353 VAAKVDTGLNKSP------------LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHS------SMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL--CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLI 2057
            ++++   GLN S             L++G+RVLV GQR G IRF G T F+PG WYGIEL+KP GKNDG+V GV+YF C    G+FAP SR+  +         I + +Q    NHS      S  T S + Q    +  N  S +++ L  S S + T         G   G V L EG QVL   +NE+G +RYVG  +F+ GIW+G+ELR   GK+DG V +KRYF C P+HG++VRPSR++ +GING+ L+
Sbjct:  460 LSSRASAGLNSSATSTANNSRCEGELRLGERVLVVGQRLGTIRFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRVQRVTDSLDTLSEISSNKQ----NHSYPGFRRSFSTTSASSQ-KEINRRNAFSKSKAALRRSWSSTPTA--------GGIEGSVKLHEGSQVLLTSSNEMGTVRYVGPTDFASGIWLGLELRSAKGKNDGSVGDKRYFTCKPNHGVLVRPSRVTYRGINGSKLV 701          

HSP 2 Score: 166.777 bits (421), Expect = 1.242e-42
Identity = 182/646 (28.17%), Postives = 262/646 (40.56%), Query Frame = 3
Query:  135 APLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQGEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYF------DVAPAVEL---LLDRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNMTTIRNNLGTLLCPTSPPGHTYYNANHRKPPETGVLSPPIRRQTPGPFPPSHLDLVSISDENEIYEAPTPVSRPVSMWRD------EIDNRNLASPSRVSAKVTLQAMXXXXXXXXXXXXXXXXSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL-CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRI 2024
            AP+  +C      FFD +   C ++L   + ++ +LFA+LRQW   VQ  ++ I  ++ K G NVNDRDGLT                                         DMT LHY          DV  AV+    L+D GADI    R     N LH AA     +  RV++           +   P D     S+   N  T  +     LC  +        AN     + G        Q P    P  +D+       E+ +A         M  D       I    L +   V+ K  L ++GL LGDRV++ G     G LR+CG  EFA G WAG+ELDEP+GKN+G+V  V YF+C  +YG+FAP++++SK     + R + +T          ++    +D++ V +KV+TGL  S         ++                PG     E  K V + D A+            G  +  S    L  R    +     +QN +S++   M+ S +           LS   S  + SS+ ST +         +S     LR G +VL     LG IR+ G  NF+ G W G+EL    GK+DG V   +YF C P +GI   PSR+
Sbjct:   38 APMPSDCE---FSFFDPNDASCQEILFDPKTSVSELFAILRQWVPQVQQNIDIIGNEILKRGCNVNDRDGLT-----------------------------------------DMTLLHYTCKSGAHGIGDVETAVKFATQLIDLGADISLRSR-WTNMNALHYAAYFDVPELIRVIL-----------KTSKPKDVDATCSDF--NFGTALHIAAYNLCAGAVKCLLEQGANPAFRNDKG--------QIPADVVPDPVDM-----PLEMADAAATAKEIKQMLLDAVPLSCNISKAMLPNYDHVTGKAMLTSLGLKLGDRVVIAGQKV--GTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGKVQYFKCAPKYGIFAPLSKISK----AKGRRKNITHTPSTKAAVPLIRSQKIDVAHVTSKVNTGLMTSKKDSASESTLS--------------LPPG-----EELKTVTEKDVAL-----------LGSVSSCSSTSSLEHRQSYPK-----KQNAISSNKKTMSKSPS-----------LSSRASAGLNSSATSTAN---------NSRCEGELRLGERVLVVGQRLGTIRFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRV 551          

HSP 3 Score: 114.39 bits (285), Expect = 1.448e-25
Identity = 66/192 (34.38%), Postives = 94/192 (48.96%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPR-NPLNP-------QTVVVDHGTVDISGVAAKVDTGLNKS--------------PLQIGDRVLVAGQRK-GVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL 1592
            G +R+ G   FA G W G+EL++P GKN G+V GV YF C  +YG+FAP +RV +V   ++      + + N   P        T       ++     +K    L +S               L  G +VL+    + G +R+VGPT+F+ G W G+EL    GKNDG+V   RYF C  N GV    SR+
Sbjct:  499 GTIRFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRVQRVTDSLDTLSEISSNKQNHSYPGFRRSFSTTSASSQKEINRRNAFSKSKAALRRSWSSTPTAGGIEGSVKLHEGSQVLLTSSNEMGTVRYVGPTDFASGIWLGLELRSAKGKNDGSVGDKRYFTCKPNHGVLVRPSRV 690          

HSP 4 Score: 62.7734 bits (151), Expect = 1.685e-9
Identity = 47/164 (28.66%), Postives = 74/164 (45.12%), Query Frame = 3
Query:  819 PETGVLSPPIRRQTPGPFPPSHLDLVS-ISDENEIYEAP---TPVSRPVSMWRDEIDNRNLASPSRVSAKVT---------LQAMXXXXXXXXXXXXXXXXSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEK 1271
            P  G+ +PP R Q         LD +S IS   + +  P      S   +  + EI+ RN  S S+ + + +         ++    L     ++    +  G +RY GP +FA G+W G+EL   +GKN G+V    YF C+  +GV    +RV+  GI   K
Sbjct:  540 PRYGIFAPPSRVQRV----TDSLDTLSEISSNKQNHSYPGFRRSFSTTSASSQKEINRRNAFSKSKAALRRSWSSTPTAGGIEGSVKLHEGSQVLLTSSNEMGTVRYVGPTDFASGIWLGLELRSAKGKNDGSVGDKRYFTCKPNHGVLVRPSRVTYRGINGSK 699          

HSP 5 Score: 55.0694 bits (131), Expect = 4.764e-7
Identity = 23/61 (37.70%), Postives = 35/61 (57.38%), Query Frame = 3
Query: 1845 VLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRIS 2027
            V+   ++G +R+ G   F+ G W G+EL    GK++G V   +YF C P +GI    S+IS
Sbjct:  290 VIAGQKVGTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGKVQYFKCAPKYGIFAPLSKIS 350          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Human
Match: CLIP4 (CAP-Gly domain containing linker protein family member 4 [Source:HGNC Symbol;Acc:HGNC:26108])

HSP 1 Score: 191.815 bits (486), Expect = 3.574e-51
Identity = 109/255 (42.75%), Postives = 148/255 (58.04%), Query Frame = 3
Query: 1353 VAAKVDTGLNKSP------------LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHS------SMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL--CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLI 2057
            ++++   GLN S             L++G+RVLV GQR G IRF G T F+PG WYGIEL+KP GKNDG+V GV+YF C    G+FAP SR+  +         I + +Q    NHS      S  T S + Q    +  N  S +++ L  S S + T         G   G V L EG QVL   +NE+G +RYVG  +F+ GIW+G+ELR   GK+DG V +KRYF C P+HG++VRPSR++ +GING+ L+
Sbjct:  460 LSSRASAGLNSSATSTANNSRCEGELRLGERVLVVGQRLGTIRFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRVQRVTDSLDTLSEISSNKQ----NHSYPGFRRSFSTTSASSQ-KEINRRNAFSKSKAALRRSWSSTPTA--------GGIEGSVKLHEGSQVLLTSSNEMGTVRYVGPTDFASGIWLGLELRSAKGKNDGSVGDKRYFTCKPNHGVLVRPSRVTYRGINGSKLV 701          

HSP 2 Score: 166.777 bits (421), Expect = 1.242e-42
Identity = 182/646 (28.17%), Postives = 262/646 (40.56%), Query Frame = 3
Query:  135 APLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQGEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYF------DVAPAVEL---LLDRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNMTTIRNNLGTLLCPTSPPGHTYYNANHRKPPETGVLSPPIRRQTPGPFPPSHLDLVSISDENEIYEAPTPVSRPVSMWRD------EIDNRNLASPSRVSAKVTLQAMXXXXXXXXXXXXXXXXSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL-CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRI 2024
            AP+  +C      FFD +   C ++L   + ++ +LFA+LRQW   VQ  ++ I  ++ K G NVNDRDGLT                                         DMT LHY          DV  AV+    L+D GADI    R     N LH AA     +  RV++           +   P D     S+   N  T  +     LC  +        AN     + G        Q P    P  +D+       E+ +A         M  D       I    L +   V+ K  L ++GL LGDRV++ G     G LR+CG  EFA G WAG+ELDEP+GKN+G+V  V YF+C  +YG+FAP++++SK     + R + +T          ++    +D++ V +KV+TGL  S         ++                PG     E  K V + D A+            G  +  S    L  R    +     +QN +S++   M+ S +           LS   S  + SS+ ST +         +S     LR G +VL     LG IR+ G  NF+ G W G+EL    GK+DG V   +YF C P +GI   PSR+
Sbjct:   38 APMPSDCE---FSFFDPNDASCQEILFDPKTSVSELFAILRQWVPQVQQNIDIIGNEILKRGCNVNDRDGLT-----------------------------------------DMTLLHYTCKSGAHGIGDVETAVKFATQLIDLGADISLRSR-WTNMNALHYAAYFDVPELIRVIL-----------KTSKPKDVDATCSDF--NFGTALHIAAYNLCAGAVKCLLEQGANPAFRNDKG--------QIPADVVPDPVDM-----PLEMADAAATAKEIKQMLLDAVPLSCNISKAMLPNYDHVTGKAMLTSLGLKLGDRVVIAGQKV--GTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGKVQYFKCAPKYGIFAPLSKISK----AKGRRKNITHTPSTKAAVPLIRSQKIDVAHVTSKVNTGLMTSKKDSASESTLS--------------LPPG-----EELKTVTEKDVAL-----------LGSVSSCSSTSSLEHRQSYPK-----KQNAISSNKKTMSKSPS-----------LSSRASAGLNSSATSTAN---------NSRCEGELRLGERVLVVGQRLGTIRFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRV 551          

HSP 3 Score: 114.39 bits (285), Expect = 1.448e-25
Identity = 66/192 (34.38%), Postives = 94/192 (48.96%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPR-NPLNP-------QTVVVDHGTVDISGVAAKVDTGLNKS--------------PLQIGDRVLVAGQRK-GVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL 1592
            G +R+ G   FA G W G+EL++P GKN G+V GV YF C  +YG+FAP +RV +V   ++      + + N   P        T       ++     +K    L +S               L  G +VL+    + G +R+VGPT+F+ G W G+EL    GKNDG+V   RYF C  N GV    SR+
Sbjct:  499 GTIRFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRVQRVTDSLDTLSEISSNKQNHSYPGFRRSFSTTSASSQKEINRRNAFSKSKAALRRSWSSTPTAGGIEGSVKLHEGSQVLLTSSNEMGTVRYVGPTDFASGIWLGLELRSAKGKNDGSVGDKRYFTCKPNHGVLVRPSRV 690          

HSP 4 Score: 62.7734 bits (151), Expect = 1.685e-9
Identity = 47/164 (28.66%), Postives = 74/164 (45.12%), Query Frame = 3
Query:  819 PETGVLSPPIRRQTPGPFPPSHLDLVS-ISDENEIYEAP---TPVSRPVSMWRDEIDNRNLASPSRVSAKVT---------LQAMXXXXXXXXXXXXXXXXSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEK 1271
            P  G+ +PP R Q         LD +S IS   + +  P      S   +  + EI+ RN  S S+ + + +         ++    L     ++    +  G +RY GP +FA G+W G+EL   +GKN G+V    YF C+  +GV    +RV+  GI   K
Sbjct:  540 PRYGIFAPPSRVQRV----TDSLDTLSEISSNKQNHSYPGFRRSFSTTSASSQKEINRRNAFSKSKAALRRSWSSTPTAGGIEGSVKLHEGSQVLLTSSNEMGTVRYVGPTDFASGIWLGLELRSAKGKNDGSVGDKRYFTCKPNHGVLVRPSRVTYRGINGSK 699          

HSP 5 Score: 55.0694 bits (131), Expect = 4.764e-7
Identity = 23/61 (37.70%), Postives = 35/61 (57.38%), Query Frame = 3
Query: 1845 VLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRIS 2027
            V+   ++G +R+ G   F+ G W G+EL    GK++G V   +YF C P +GI    S+IS
Sbjct:  290 VIAGQKVGTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGKVQYFKCAPKYGIFAPLSKIS 350          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Human
Match: CLIP3 (CAP-Gly domain containing linker protein 3 [Source:HGNC Symbol;Acc:HGNC:24314])

HSP 1 Score: 166.777 bits (421), Expect = 1.885e-43
Identity = 87/186 (46.77%), Postives = 115/186 (61.83%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSP---------------LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLH 1595
            +G LR+CG  EFA G W GVELDEP+GKN G+V GV YF C  + G+FA V+++SK    V+  P  VT      P+T  +D   V  +G   +   G  K+P                ++GD+VLVAGQ++G++RF G T+F+PG WYGIELD+P GK+DG+V GVRYF C    GVFAP SR+ 
Sbjct:  307 TGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKQGLFASVSKISKA---VDAPPSSVTS----TPRTPRMDFSRV--TGKGRREHKGKKKTPSSPSLGSLQQRDGAKAEVGDQVLVAGQKQGIVRFYGKTDFAPGYWYGIELDQPTGKHDGSVFGVRYFTCPPRHGVFAPASRIQ 483          

HSP 2 Score: 161.77 bits (408), Expect = 1.135e-41
Identity = 86/208 (41.35%), Postives = 125/208 (60.10%), Query Frame = 3
Query:   96 ERRKYPETHPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLLDRGADIRAI---CRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLP 704
            ERR+ P  HP+  APL     +Y   FFD +   C ++L   + TI +LFA++RQW   VQ +++ I  ++ + G +VNDRDGLTDMT+LHYA K+GA   G+   AVR+   LL  GA +  R +WT+M  LHYA YFDV   V +LL +GA  R +   C +   G+ LHIAA+ L L  A+ L++ GA+    + + + P + +P
Sbjct:   36 ERRQKPVVHPSAPAPLPK---DYAFTFFDPNDPACQEILFDPQTTIPELFAIVRQWVPQVQHKIDVIGNEILRRGCHVNDRDGLTDMTLLHYACKAGAHGVGDPAAAVRLSQQLLALGADVTLRSRWTNMNALHYAAYFDVPDLVRVLL-KGARPRVVNSTCSDFNHGSALHIAASSLCLGAAKCLLEHGANPALRNRKGQVPAEVVP 239          

HSP 3 Score: 139.428 bits (350), Expect = 4.533e-34
Identity = 78/216 (36.11%), Postives = 112/216 (51.85%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL-CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKG 2036
            L++GDRVL+ GQ+ G +RF G TEF+ G W G+ELD+P GKNDG+V GVRYF C   +G+FA +S+  I ++       + +T +    + S +    + +      +P+  S+           S   +  +    GD           QVL    + GI+R+ G  +F+ G W G+EL   TGKHDG V   RYF CPP HG+    SRI   G
Sbjct:  294 LRLGDRVLLDGQKTGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKQGLFASVSK--ISKAVDAPPSSVTSTPRTPRMDFSRVTGKGRREHKGKKKTPSSPSLG----------SLQQRDGAKAEVGD-----------QVLVAGQKQGIVRFYGKTDFAPGYWYGIELDQPTGKHDGSVFGVRYFTCPPRHGVFAPASRIQRIG 486          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Human
Match: CLIP3 (CAP-Gly domain containing linker protein 3 [Source:HGNC Symbol;Acc:HGNC:24314])

HSP 1 Score: 166.777 bits (421), Expect = 1.885e-43
Identity = 87/186 (46.77%), Postives = 115/186 (61.83%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSP---------------LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLH 1595
            +G LR+CG  EFA G W GVELDEP+GKN G+V GV YF C  + G+FA V+++SK    V+  P  VT      P+T  +D   V  +G   +   G  K+P                ++GD+VLVAGQ++G++RF G T+F+PG WYGIELD+P GK+DG+V GVRYF C    GVFAP SR+ 
Sbjct:  307 TGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKQGLFASVSKISKA---VDAPPSSVTS----TPRTPRMDFSRV--TGKGRREHKGKKKTPSSPSLGSLQQRDGAKAEVGDQVLVAGQKQGIVRFYGKTDFAPGYWYGIELDQPTGKHDGSVFGVRYFTCPPRHGVFAPASRIQ 483          

HSP 2 Score: 161.77 bits (408), Expect = 1.135e-41
Identity = 86/208 (41.35%), Postives = 125/208 (60.10%), Query Frame = 3
Query:   96 ERRKYPETHPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLLDRGADIRAI---CRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLP 704
            ERR+ P  HP+  APL     +Y   FFD +   C ++L   + TI +LFA++RQW   VQ +++ I  ++ + G +VNDRDGLTDMT+LHYA K+GA   G+   AVR+   LL  GA +  R +WT+M  LHYA YFDV   V +LL +GA  R +   C +   G+ LHIAA+ L L  A+ L++ GA+    + + + P + +P
Sbjct:   36 ERRQKPVVHPSAPAPLPK---DYAFTFFDPNDPACQEILFDPQTTIPELFAIVRQWVPQVQHKIDVIGNEILRRGCHVNDRDGLTDMTLLHYACKAGAHGVGDPAAAVRLSQQLLALGADVTLRSRWTNMNALHYAAYFDVPDLVRVLL-KGARPRVVNSTCSDFNHGSALHIAASSLCLGAAKCLLEHGANPALRNRKGQVPAEVVP 239          

HSP 3 Score: 139.428 bits (350), Expect = 4.533e-34
Identity = 78/216 (36.11%), Postives = 112/216 (51.85%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL-CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKG 2036
            L++GDRVL+ GQ+ G +RF G TEF+ G W G+ELD+P GKNDG+V GVRYF C   +G+FA +S+  I ++       + +T +    + S +    + +      +P+  S+           S   +  +    GD           QVL    + GI+R+ G  +F+ G W G+EL   TGKHDG V   RYF CPP HG+    SRI   G
Sbjct:  294 LRLGDRVLLDGQKTGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKQGLFASVSK--ISKAVDAPPSSVTSTPRTPRMDFSRVTGKGRREHKGKKKTPSSPSLG----------SLQQRDGAKAEVGD-----------QVLVAGQKQGIVRFYGKTDFAPGYWYGIELDQPTGKHDGSVFGVRYFTCPPRHGVFAPASRIQRIG 486          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Human
Match: CLIP4 (CAP-Gly domain containing linker protein family member 4 [Source:HGNC Symbol;Acc:HGNC:26108])

HSP 1 Score: 166.777 bits (421), Expect = 4.409e-43
Identity = 182/646 (28.17%), Postives = 262/646 (40.56%), Query Frame = 3
Query:  135 APLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQGEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYF------DVAPAVEL---LLDRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNMTTIRNNLGTLLCPTSPPGHTYYNANHRKPPETGVLSPPIRRQTPGPFPPSHLDLVSISDENEIYEAPTPVSRPVSMWRD------EIDNRNLASPSRVSAKVTLQAMXXXXXXXXXXXXXXXXSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL-CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRI 2024
            AP+  +C      FFD +   C ++L   + ++ +LFA+LRQW   VQ  ++ I  ++ K G NVNDRDGLT                                         DMT LHY          DV  AV+    L+D GADI    R     N LH AA     +  RV++           +   P D     S+   N  T  +     LC  +        AN     + G        Q P    P  +D+       E+ +A         M  D       I    L +   V+ K  L ++GL LGDRV++ G     G LR+CG  EFA G WAG+ELDEP+GKN+G+V  V YF+C  +YG+FAP++++SK     + R + +T          ++    +D++ V +KV+TGL  S         ++                PG     E  K V + D A+            G  +  S    L  R    +     +QN +S++   M+ S +           LS   S  + SS+ ST +         +S     LR G +VL     LG IR+ G  NF+ G W G+EL    GK+DG V   +YF C P +GI   PSR+
Sbjct:   38 APMPSDCE---FSFFDPNDASCQEILFDPKTSVSELFAILRQWVPQVQQNIDIIGNEILKRGCNVNDRDGLT-----------------------------------------DMTLLHYTCKSGAHGIGDVETAVKFATQLIDLGADISLRSR-WTNMNALHYAAYFDVPELIRVIL-----------KTSKPKDVDATCSDF--NFGTALHIAAYNLCAGAVKCLLEQGANPAFRNDKG--------QIPADVVPDPVDM-----PLEMADAAATAKEIKQMLLDAVPLSCNISKAMLPNYDHVTGKAMLTSLGLKLGDRVVIAGQKV--GTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGKVQYFKCAPKYGIFAPLSKISK----AKGRRKNITHTPSTKAAVPLIRSQKIDVAHVTSKVNTGLMTSKKDSASESTLS--------------LPPG-----EELKTVTEKDVAL-----------LGSVSSCSSTSSLEHRQSYPK-----KQNAISSNKKTMSKSPS-----------LSSRASAGLNSSATSTAN---------NSRCEGELRLGERVLVVGQRLGTIRFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRV 551          

HSP 2 Score: 100.523 bits (249), Expect = 2.755e-21
Identity = 46/86 (53.49%), Postives = 58/86 (67.44%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQN 1649
            L++G+RVLV GQR G IRF G T F+PG WYGIEL+KP GKNDG+V GV+YF C    G+FAP SR+  +         I + +QN
Sbjct:  485 LRLGERVLVVGQRLGTIRFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRVQRVTDSLDTLSEISSNKQN 570          

HSP 3 Score: 73.1738 bits (178), Expect = 9.578e-13
Identity = 29/56 (51.79%), Postives = 39/56 (69.64%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKV 1253
            G +R+ G   FA G W G+EL++P GKN G+V GV YF C  +YG+FAP +RV +V
Sbjct:  499 GTIRFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRVQRV 554          

HSP 4 Score: 55.0694 bits (131), Expect = 3.968e-7
Identity = 23/61 (37.70%), Postives = 35/61 (57.38%), Query Frame = 3
Query: 1845 VLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRIS 2027
            V+   ++G +R+ G   F+ G W G+EL    GK++G V   +YF C P +GI    S+IS
Sbjct:  290 VIAGQKVGTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGKVQYFKCAPKYGIFAPLSKIS 350          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Celegans
Match: tbcb-1 (Tubulin-specific chaperone B [Source:UniProtKB/Swiss-Prot;Acc:Q20728])

HSP 1 Score: 65.0846 bits (157), Expect = 1.415e-11
Identity = 31/65 (47.69%), Postives = 42/65 (64.62%), Query Frame = 3
Query: 1392 LQIGDR--VLVAGQ--RKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVF 1574
            + +G+R  V V  Q  R+G + +VG T+F  G W G++ D+PVGKNDG+V GVRYF C    G F
Sbjct:  146 IMVGNRCEVTVGAQMARRGEVAYVGATKFKEGVWVGVKYDEPVGKNDGSVAGVRYFDCDPKYGGF 210          

HSP 2 Score: 59.6918 bits (143), Expect = 1.142e-9
Identity = 25/47 (53.19%), Postives = 32/47 (68.09%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVF 1226
            G + Y G  +F +GVW GV+ DEP GKN G+V GV YF C+ +YG F
Sbjct:  164 GEVAYVGATKFKEGVWVGVKYDEPVGKNDGSVAGVRYFDCDPKYGGF 210          

HSP 3 Score: 59.6918 bits (143), Expect = 1.164e-9
Identity = 26/55 (47.27%), Postives = 33/55 (60.00%), Query Frame = 3
Query: 1866 GIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISV 2030
            G + YVGA  F +G+WVGV+     GK+DG V   RYF C P +G  VRP  + V
Sbjct:  164 GEVAYVGATKFKEGVWVGVKYDEPVGKNDGSVAGVRYFDCDPKYGGFVRPVDVKV 218          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Celegans
Match: dnc-1 (DyNactin Complex component [Source:UniProtKB/TrEMBL;Acc:E2JKZ0])

HSP 1 Score: 65.4698 bits (158), Expect = 1.478e-10
Identity = 41/121 (33.88%), Postives = 62/121 (51.24%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMT 1754
             +IG RV  +    G + F G T+F+ G W G+ LD   GKN+G V  V+YF+C  N GVF   S + +  +  ++   +KA   + +   SS+M+ S   + S  SSP     A S  +T
Sbjct:    3 FEIGTRVKTSSG-NGRVVFCGQTQFAEGDWVGVILDTATGKNNGTVQNVQYFECEPNFGVFVKSSAVELEDASKRSG--LKAPAASAIRKDSSVMSRSAGSKASPGSSPGMSPAASSEKLT 120          

HSP 2 Score: 62.7734 bits (151), Expect = 9.549e-10
Identity = 26/54 (48.15%), Postives = 35/54 (64.81%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRV 1244
            +G + +CG  +FA+G W GV LD   GKN+GTV  V YF+CE  +GVF   + V
Sbjct:   15 NGRVVFCGQTQFAEGDWVGVILDTATGKNNGTVQNVQYFECEPNFGVFVKSSAV 68          

HSP 3 Score: 54.6842 bits (130), Expect = 2.961e-7
Identity = 24/66 (36.36%), Postives = 40/66 (60.61%), Query Frame = 3
Query: 1836 GLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVK 2033
            G +V  ++  G + + G   F++G WVGV L   TGK++G V N +YF C P+ G+ V+ S + ++
Sbjct:    6 GTRVKTSSGNGRVVFCGQTQFAEGDWVGVILDTATGKNNGTVQNVQYFECEPNFGVFVKSSAVELE 71          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Celegans
Match: dnc-1 (DyNactin Complex component [Source:UniProtKB/TrEMBL;Acc:E2JKZ0])

HSP 1 Score: 65.4698 bits (158), Expect = 1.484e-10
Identity = 41/121 (33.88%), Postives = 62/121 (51.24%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMT 1754
             +IG RV  +    G + F G T+F+ G W G+ LD   GKN+G V  V+YF+C  N GVF   S + +  +  ++   +KA   + +   SS+M+ S   + S  SSP     A S  +T
Sbjct:    3 FEIGTRVKTSSG-NGRVVFCGQTQFAEGDWVGVILDTATGKNNGTVQNVQYFECEPNFGVFVKSSAVELEDASKRSG--LKAPAASAIRKDSSVMSRSAGSKASPGSSPGMSPAASSEKLT 120          

HSP 2 Score: 62.7734 bits (151), Expect = 9.752e-10
Identity = 26/54 (48.15%), Postives = 35/54 (64.81%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRV 1244
            +G + +CG  +FA+G W GV LD   GKN+GTV  V YF+CE  +GVF   + V
Sbjct:   15 NGRVVFCGQTQFAEGDWVGVILDTATGKNNGTVQNVQYFECEPNFGVFVKSSAV 68          

HSP 3 Score: 54.6842 bits (130), Expect = 3.079e-7
Identity = 24/66 (36.36%), Postives = 40/66 (60.61%), Query Frame = 3
Query: 1836 GLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVK 2033
            G +V  ++  G + + G   F++G WVGV L   TGK++G V N +YF C P+ G+ V+ S + ++
Sbjct:    6 GTRVKTSSGNGRVVFCGQTQFAEGDWVGVILDTATGKNNGTVQNVQYFECEPNFGVFVKSSAVELE 71          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Celegans
Match: dnc-1 (DyNactin Complex component [Source:UniProtKB/TrEMBL;Acc:E2JKZ0])

HSP 1 Score: 65.0846 bits (157), Expect = 1.563e-10
Identity = 41/121 (33.88%), Postives = 62/121 (51.24%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMT 1754
             +IG RV  +    G + F G T+F+ G W G+ LD   GKN+G V  V+YF+C  N GVF   S + +  +  ++   +KA   + +   SS+M+ S   + S  SSP     A S  +T
Sbjct:    3 FEIGTRVKTSSG-NGRVVFCGQTQFAEGDWVGVILDTATGKNNGTVQNVQYFECEPNFGVFVKSSAVELEDASKRSG--LKAPAASAIRKDSSVMSRSAGSKASPGSSPGMSPAASSEKLT 120          

HSP 2 Score: 62.7734 bits (151), Expect = 9.432e-10
Identity = 26/54 (48.15%), Postives = 35/54 (64.81%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRV 1244
            +G + +CG  +FA+G W GV LD   GKN+GTV  V YF+CE  +GVF   + V
Sbjct:   15 NGRVVFCGQTQFAEGDWVGVILDTATGKNNGTVQNVQYFECEPNFGVFVKSSAV 68          

HSP 3 Score: 54.6842 bits (130), Expect = 3.102e-7
Identity = 24/66 (36.36%), Postives = 40/66 (60.61%), Query Frame = 3
Query: 1836 GLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVK 2033
            G +V  ++  G + + G   F++G WVGV L   TGK++G V N +YF C P+ G+ V+ S + ++
Sbjct:    6 GTRVKTSSGNGRVVFCGQTQFAEGDWVGVILDTATGKNNGTVQNVQYFECEPNFGVFVKSSAVELE 71          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Celegans
Match: dnc-1 (DyNactin Complex component [Source:UniProtKB/TrEMBL;Acc:E2JKZ0])

HSP 1 Score: 65.0846 bits (157), Expect = 1.570e-10
Identity = 41/121 (33.88%), Postives = 62/121 (51.24%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMT 1754
             +IG RV  +    G + F G T+F+ G W G+ LD   GKN+G V  V+YF+C  N GVF   S + +  +  ++   +KA   + +   SS+M+ S   + S  SSP     A S  +T
Sbjct:    3 FEIGTRVKTSSG-NGRVVFCGQTQFAEGDWVGVILDTATGKNNGTVQNVQYFECEPNFGVFVKSSAVELEDASKRSG--LKAPAASAIRKDSSVMSRSAGSKASPGSSPGMSPAASSEKLT 120          

HSP 2 Score: 62.7734 bits (151), Expect = 9.635e-10
Identity = 26/54 (48.15%), Postives = 35/54 (64.81%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRV 1244
            +G + +CG  +FA+G W GV LD   GKN+GTV  V YF+CE  +GVF   + V
Sbjct:   15 NGRVVFCGQTQFAEGDWVGVILDTATGKNNGTVQNVQYFECEPNFGVFVKSSAV 68          

HSP 3 Score: 54.299 bits (129), Expect = 3.171e-7
Identity = 24/66 (36.36%), Postives = 40/66 (60.61%), Query Frame = 3
Query: 1836 GLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVK 2033
            G +V  ++  G + + G   F++G WVGV L   TGK++G V N +YF C P+ G+ V+ S + ++
Sbjct:    6 GTRVKTSSGNGRVVFCGQTQFAEGDWVGVILDTATGKNNGTVQNVQYFECEPNFGVFVKSSAVELE 71          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Fly
Match: CLIP-190 (gene:FBgn0020503 transcript:FBtr0332455)

HSP 1 Score: 138.272 bits (347), Expect = 8.065e-36
Identity = 76/225 (33.78%), Postives = 122/225 (54.22%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQIGDRVLVA---GQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLM 1751
            G + + G   FA G WAGV LDEP GKN G V+G  YFQCE + G+F+ +TR++   +   + P   +P    +P        T  I     +      K+ + +GDRV+V+   G R G++R++G T+F+PG W G+ELD+P GKNDG V+ +RYF+C    GVF P++++ +  S  K       T+ +R  +  S+ +    + ++  ++      AQ  L+
Sbjct:   45 GQIAFIGDTHFAAGEWAGVVLDEPNGKNDGCVSGKRYFQCEPKRGIFSRLTRLTTYPLAGAQTP--TSPLAKSSPDRSRTVSPTASIRSSMLRSPGIGGKNGMAVGDRVIVSSGFGSRPGILRYLGETQFAPGNWCGVELDEPSGKNDGTVDDIRYFECKPKYGVFVPIAKVSLSPSSKK-------TRLSRTGSRESLTSIGTMNSIATTATSRMRMNAQQILI 260          

HSP 2 Score: 117.087 bits (292), Expect = 1.525e-28
Identity = 78/226 (34.51%), Postives = 107/226 (47.35%), Query Frame = 3
Query: 1380 NKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTY----FGDSSG-----RVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISV 2030
            N     IG RV + G R G I F+G T F+ G W G+ LD+P GKNDG V+G RYFQC   RG+F+ L+RL                                T  L+ A +P    +A+S    S +VS T    S        G  +G     RV +  G      +  GI+RY+G   F+ G W GVEL   +GK+DG V + RYF C P +G+ V  +++S+
Sbjct:   27 NTEQFIIGQRVWLGGTRPGQIAFIGDTHFAAGEWAGVVLDEPNGKNDGCVSGKRYFQCEPKRGIFSRLTRL-------------------------------TTYPLAGAQTPTS-PLAKSSPDRSRTVSPTASIRSSMLRSPGIGGKNGMAVGDRVIVSSGF----GSRPGILRYLGETQFAPGNWCGVELDEPSGKNDGTVDDIRYFECKPKYGVFVPIAKVSL 216          

HSP 3 Score: 56.225 bits (134), Expect = 4.600e-8
Identity = 28/69 (40.58%), Postives = 37/69 (53.62%), Query Frame = 3
Query: 1866 GIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLIPPELA 2072
            G I ++G  +F+ G W GV L    GK+DG V  KRYF C P  GI  R +R++   + GA      LA
Sbjct:   45 GQIAFIGDTHFAAGEWAGVVLDEPNGKNDGCVSGKRYFQCEPKRGIFSRLTRLTTYPLAGAQTPTSPLA 113          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Fly
Match: CLIP-190 (gene:FBgn0020503 transcript:FBtr0332452)

HSP 1 Score: 137.887 bits (346), Expect = 1.037e-35
Identity = 70/180 (38.89%), Postives = 105/180 (58.33%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQIGDRVLVA---GQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHK 1616
            G + + G   FA G WAGV LDEP GKN G V+G  YFQCE + G+F+ +TR++   +   + P   +P    +P        T  I     +      K+ + +GDRV+V+   G R G++R++G T+F+PG W G+ELD+P GKNDG V+ +RYF+C    GVF P++++ +  S  K
Sbjct:   99 GQIAFIGDTHFAAGEWAGVVLDEPNGKNDGCVSGKRYFQCEPKRGIFSRLTRLTTYPLAGAQTP--TSPLAKSSPDRSRTVSPTASIRSSMLRSPGIGGKNGMAVGDRVIVSSGFGSRPGILRYLGETQFAPGNWCGVELDEPSGKNDGTVDDIRYFECKPKYGVFVPIAKVSLSPSSKK 276          

HSP 2 Score: 117.472 bits (293), Expect = 1.223e-28
Identity = 75/217 (34.56%), Postives = 102/217 (47.00%), Query Frame = 3
Query: 1380 NKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISV 2030
            N     IG RV + G R G I F+G T F+ G W G+ LD+P GKNDG V+G RYFQC   RG+F+ L+RL                           +  +QT     A S    S   SP  +  S        SP   G +   V  R  +     +  GI+RY+G   F+ G W GVEL   +GK+DG V + RYF C P +G+ V  +++S+
Sbjct:   81 NTEQFIIGQRVWLGGTRPGQIAFIGDTHFAAGEWAGVVLDEPNGKNDGCVSGKRYFQCEPKRGIFSRLTRLTTY-----------------------PLAGAQTPTSPLAKSSPDRSRTVSPTASIRS----SMLRSPGIGGKNGMAVGDRVIVSSGFGSRPGILRYLGETQFAPGNWCGVELDEPSGKNDGTVDDIRYFECKPKYGVFVPIAKVSL 270          

HSP 3 Score: 57.3806 bits (137), Expect = 1.953e-8
Identity = 28/69 (40.58%), Postives = 37/69 (53.62%), Query Frame = 3
Query: 1866 GIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLIPPELA 2072
            G I ++G  +F+ G W GV L    GK+DG V  KRYF C P  GI  R +R++   + GA      LA
Sbjct:   99 GQIAFIGDTHFAAGEWAGVVLDEPNGKNDGCVSGKRYFQCEPKRGIFSRLTRLTTYPLAGAQTPTSPLA 167          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Fly
Match: CLIP-190 (gene:FBgn0020503 transcript:FBtr0290263)

HSP 1 Score: 137.887 bits (346), Expect = 1.939e-35
Identity = 70/181 (38.67%), Postives = 105/181 (58.01%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQIGDRVLVA---GQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKN 1619
            G + + G   FA G WAGV LDEP GKN G V+G  YFQCE + G+F+ +TR++   +   + P   +P    +P        T  I     +      K+ + +GDRV+V+   G R G++R++G T+F+PG W G+ELD+P GKNDG V+ +RYF+C    GVF P++++ +  S  K 
Sbjct:   45 GQIAFIGDTHFAAGEWAGVVLDEPNGKNDGCVSGKRYFQCEPKRGIFSRLTRLTTYPLAGAQTP--TSPLAKSSPDRSRTVSPTASIRSSMLRSPGIGGKNGMAVGDRVIVSSGFGSRPGILRYLGETQFAPGNWCGVELDEPSGKNDGTVDDIRYFECKPKYGVFVPIAKVSLSPSSKKT 223          

HSP 2 Score: 117.472 bits (293), Expect = 1.866e-28
Identity = 78/226 (34.51%), Postives = 107/226 (47.35%), Query Frame = 3
Query: 1380 NKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTY----FGDSSG-----RVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISV 2030
            N     IG RV + G R G I F+G T F+ G W G+ LD+P GKNDG V+G RYFQC   RG+F+ L+RL                                T  L+ A +P    +A+S    S +VS T    S        G  +G     RV +  G      +  GI+RY+G   F+ G W GVEL   +GK+DG V + RYF C P +G+ V  +++S+
Sbjct:   27 NTEQFIIGQRVWLGGTRPGQIAFIGDTHFAAGEWAGVVLDEPNGKNDGCVSGKRYFQCEPKRGIFSRLTRL-------------------------------TTYPLAGAQTPTS-PLAKSSPDRSRTVSPTASIRSSMLRSPGIGGKNGMAVGDRVIVSSGF----GSRPGILRYLGETQFAPGNWCGVELDEPSGKNDGTVDDIRYFECKPKYGVFVPIAKVSL 216          

HSP 3 Score: 56.225 bits (134), Expect = 4.161e-8
Identity = 28/69 (40.58%), Postives = 37/69 (53.62%), Query Frame = 3
Query: 1866 GIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLIPPELA 2072
            G I ++G  +F+ G W GV L    GK+DG V  KRYF C P  GI  R +R++   + GA      LA
Sbjct:   45 GQIAFIGDTHFAAGEWAGVVLDEPNGKNDGCVSGKRYFQCEPKRGIFSRLTRLTTYPLAGAQTPTSPLA 113          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Fly
Match: CLIP-190 (gene:FBgn0020503 transcript:FBtr0339796)

HSP 1 Score: 137.887 bits (346), Expect = 1.952e-35
Identity = 70/181 (38.67%), Postives = 105/181 (58.01%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQIGDRVLVA---GQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKN 1619
            G + + G   FA G WAGV LDEP GKN G V+G  YFQCE + G+F+ +TR++   +   + P   +P    +P        T  I     +      K+ + +GDRV+V+   G R G++R++G T+F+PG W G+ELD+P GKNDG V+ +RYF+C    GVF P++++ +  S  K 
Sbjct:   45 GQIAFIGDTHFAAGEWAGVVLDEPNGKNDGCVSGKRYFQCEPKRGIFSRLTRLTTYPLAGAQTP--TSPLAKSSPDRSRTVSPTASIRSSMLRSPGIGGKNGMAVGDRVIVSSGFGSRPGILRYLGETQFAPGNWCGVELDEPSGKNDGTVDDIRYFECKPKYGVFVPIAKVSLSPSSKKT 223          

HSP 2 Score: 117.472 bits (293), Expect = 1.901e-28
Identity = 78/226 (34.51%), Postives = 107/226 (47.35%), Query Frame = 3
Query: 1380 NKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTY----FGDSSG-----RVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISV 2030
            N     IG RV + G R G I F+G T F+ G W G+ LD+P GKNDG V+G RYFQC   RG+F+ L+RL                                T  L+ A +P    +A+S    S +VS T    S        G  +G     RV +  G      +  GI+RY+G   F+ G W GVEL   +GK+DG V + RYF C P +G+ V  +++S+
Sbjct:   27 NTEQFIIGQRVWLGGTRPGQIAFIGDTHFAAGEWAGVVLDEPNGKNDGCVSGKRYFQCEPKRGIFSRLTRL-------------------------------TTYPLAGAQTPTS-PLAKSSPDRSRTVSPTASIRSSMLRSPGIGGKNGMAVGDRVIVSSGF----GSRPGILRYLGETQFAPGNWCGVELDEPSGKNDGTVDDIRYFECKPKYGVFVPIAKVSL 216          

HSP 3 Score: 56.225 bits (134), Expect = 4.327e-8
Identity = 28/69 (40.58%), Postives = 37/69 (53.62%), Query Frame = 3
Query: 1866 GIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLIPPELA 2072
            G I ++G  +F+ G W GV L    GK+DG V  KRYF C P  GI  R +R++   + GA      LA
Sbjct:   45 GQIAFIGDTHFAAGEWAGVVLDEPNGKNDGCVSGKRYFQCEPKRGIFSRLTRLTTYPLAGAQTPTSPLA 113          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Fly
Match: CLIP-190 (gene:FBgn0020503 transcript:FBtr0290262)

HSP 1 Score: 137.887 bits (346), Expect = 3.604e-35
Identity = 70/180 (38.89%), Postives = 105/180 (58.33%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQIGDRVLVA---GQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHK 1616
            G + + G   FA G WAGV LDEP GKN G V+G  YFQCE + G+F+ +TR++   +   + P   +P    +P        T  I     +      K+ + +GDRV+V+   G R G++R++G T+F+PG W G+ELD+P GKNDG V+ +RYF+C    GVF P++++ +  S  K
Sbjct:   45 GQIAFIGDTHFAAGEWAGVVLDEPNGKNDGCVSGKRYFQCEPKRGIFSRLTRLTTYPLAGAQTP--TSPLAKSSPDRSRTVSPTASIRSSMLRSPGIGGKNGMAVGDRVIVSSGFGSRPGILRYLGETQFAPGNWCGVELDEPSGKNDGTVDDIRYFECKPKYGVFVPIAKVSLSPSSKK 222          

HSP 2 Score: 117.857 bits (294), Expect = 3.113e-28
Identity = 78/226 (34.51%), Postives = 107/226 (47.35%), Query Frame = 3
Query: 1380 NKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTY----FGDSSG-----RVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISV 2030
            N     IG RV + G R G I F+G T F+ G W G+ LD+P GKNDG V+G RYFQC   RG+F+ L+RL                                T  L+ A +P    +A+S    S +VS T    S        G  +G     RV +  G      +  GI+RY+G   F+ G W GVEL   +GK+DG V + RYF C P +G+ V  +++S+
Sbjct:   27 NTEQFIIGQRVWLGGTRPGQIAFIGDTHFAAGEWAGVVLDEPNGKNDGCVSGKRYFQCEPKRGIFSRLTRL-------------------------------TTYPLAGAQTPTS-PLAKSSPDRSRTVSPTASIRSSMLRSPGIGGKNGMAVGDRVIVSSGF----GSRPGILRYLGETQFAPGNWCGVELDEPSGKNDGTVDDIRYFECKPKYGVFVPIAKVSL 216          

HSP 3 Score: 56.6102 bits (135), Expect = 4.374e-8
Identity = 26/62 (41.94%), Postives = 35/62 (56.45%), Query Frame = 3
Query: 1866 GIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGAN 2051
            G I ++G  +F+ G W GV L    GK+DG V  KRYF C P  GI  R +R++   + GA 
Sbjct:   45 GQIAFIGDTHFAAGEWAGVVLDEPNGKNDGCVSGKRYFQCEPKRGIFSRLTRLTTYPLAGAQ 106          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Zebrafish
Match: si:dkeyp-47f9.4 (si:dkeyp-47f9.4 [Source:ZFIN;Acc:ZDB-GENE-131126-9])

HSP 1 Score: 174.096 bits (440), Expect = 1.071e-46
Identity = 102/245 (41.63%), Postives = 141/245 (57.55%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMM--TASQTDQLSNASSPNHLSMAQSPLMTS-----SSVST---THQFNS-----PTYFG------DSSGRVFLREGLQVL--CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLI 2057
            + +G+RVLV GQR GV+RF G T F+PG W GIELDKP GKNDG+V GVRYF C    GVFAP SR+  +   H + + +     +R++   + +  T S +  ++    P+  S A      +     S+VS      + NS     P   G       S G V L  G+QVL    NE+  IRY+G   F+ G+W+G+ELR   GK+DG V ++RYF C P HG++VRPSR++ +GINGA L+
Sbjct:  236 VHLGERVLVVGQRTGVVRFYGKTSFAPGFWLGIELDKPGGKNDGSVGGVRYFSCPPKHGVFAPPSRVQRI---HGSVDCLSELSSSRMNYSFTGIRRTFSTSSAIATQREPSRKSQASRNRSNNLRRRWSTVSAGWGASRANSSAGPGPIRVGGNTSCPGSDGTVKLHLGMQVLLISANEMATIRYIGTAEFAPGLWLGLELRSPKGKNDGSVGSRRYFSCRPGHGVLVRPSRVTYRGINGAKLV 477          

HSP 2 Score: 131.724 bits (330), Expect = 3.829e-32
Identity = 86/260 (33.08%), Postives = 124/260 (47.69%), Query Frame = 3
Query: 1362 KVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKND--------------EIIKATQQ---------------NRLSNHSSMMTASQTDQLSNASSPNHLSMAQS----PLMTSSSVSTTHQF--NSPTYFGDSSG----RVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRI 2024
            K  T L+   L +GD+V++AGQ+ G +RF G TEF+ G W G+EL +PVGKNDG+V GV+YF C    G+FAPLS++  L    K+               ++ + T +               +  S  +S        +      P    +A S    P  + S  ST H     S T    S+      V L E + V+     G++R+ G  +F+ G W+G+EL    GK+DG V   RYF CPP HG+   PSR+
Sbjct:   44 KARTQLSSMGLHLGDKVVIAGQKVGSLRFCGSTEFAGGLWAGVELQQPVGKNDGSVAGVQYFTCPIKHGIFAPLSKISKLSEHRKHSPRQTCVPVPHPRRIDLARVTSKVNTGLLSRSCSTSSSSLDSRQTSRRPPRPLPRQRPPLHPWQERVAPSTRSTPAPSRSHTSTPHSIIARSRTPLASSTAHESPEVHLGERVLVV-GQRTGVVRFYGKTSFAPGFWLGIELDKPGGKNDGSVGGVRYFSCPPKHGVFAPPSRV 302          

HSP 3 Score: 108.997 bits (271), Expect = 1.258e-24
Identity = 63/218 (28.90%), Postives = 96/218 (44.04%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKV------------------------------GIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQIGDR------------------VLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL 1592
            +G++R+ G   FA G W G+ELD+P GKN G+V GV YF C  ++GVFAP +RV ++                               I  ++ P   +  +      +     TV     A++ ++     P+++G                    +L++      IR++G  EF+PG W G+EL  P GKNDG+V   RYF C    GV    SR+
Sbjct:  249 TGVVRFYGKTSFAPGFWLGIELDKPGGKNDGSVGGVRYFSCPPKHGVFAPPSRVQRIHGSVDCLSELSSSRMNYSFTGIRRTFSTSSAIATQREPSRKSQASRNRSNNLRRRWSTVSAGWGASRANSSAGPGPIRVGGNTSCPGSDGTVKLHLGMQVLLISANEMATIRYIGTAEFAPGLWLGLELRSPKGKNDGSVGSRRYFSCRPGHGVLVRPSRV 466          

HSP 4 Score: 85.1149 bits (209), Expect = 6.960e-17
Identity = 43/99 (43.43%), Postives = 62/99 (62.63%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPR--CVTPRNPLNPQTVVVDHGTVDISGVAAKVDTG 1376
            G LR+CG  EFA G+WAGVEL +P GKN G+V GV YF C  ++G+FAP++++SK+    +  PR  CV   +P            +D++ V +KV+TG
Sbjct:   68 GSLRFCGSTEFAGGLWAGVELQQPVGKNDGSVAGVQYFTCPIKHGIFAPLSKISKLSEHRKHSPRQTCVPVPHPRR----------IDLARVTSKVNTG 156          

HSP 5 Score: 62.7734 bits (151), Expect = 1.010e-9
Identity = 26/61 (42.62%), Postives = 38/61 (62.30%), Query Frame = 3
Query: 1845 VLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRIS 2027
            V+   ++G +R+ G+  F+ G+W GVEL+   GK+DG V   +YF CP  HGI    S+IS
Sbjct:   61 VIAGQKVGSLRFCGSTEFAGGLWAGVELQQPVGKNDGSVAGVQYFTCPIKHGIFAPLSKIS 121          

HSP 6 Score: 58.151 bits (139), Expect = 2.867e-8
Identity = 27/60 (45.00%), Postives = 35/60 (58.33%), Query Frame = 3
Query: 1092 LRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEK 1271
            +RY G  EFA G+W G+EL  P+GKN G+V    YF C   +GV    +RV+  GI   K
Sbjct:  416 IRYIGTAEFAPGLWLGLELRSPKGKNDGSVGSRRYFSCRPGHGVLVRPSRVTYRGINGAK 475          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Zebrafish
Match: clip3 (CAP-GLY domain containing linker protein 3 [Source:ZFIN;Acc:ZDB-GENE-131127-193])

HSP 1 Score: 172.17 bits (435), Expect = 1.686e-45
Identity = 87/212 (41.04%), Postives = 127/212 (59.91%), Query Frame = 3
Query:   81 VDTISERRKYPETHPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLL--DRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLP 704
            V  + E RK P  HPA +APL     +Y   FFD +   CM++L   R TI +LFA++RQW   VQ +++ I  ++ K G +VNDRDGLTDMT+LHY  K+GA   G+ E A+R+   LL +GA +  R +WT+M  LHYA YFDV   + +LL   +   + + C + C G  LHIAA+ L L   + L++ GA+    +D+ + P + +P
Sbjct:   19 VSPVHEPRKKPMVHPAAQAPLP---KDYAFTFFDPNDPACMEILTDPRTTIPELFAIIRQWVPQVQHKIDIIGNEILKRGCHVNDRDGLTDMTLLHYCCKAGAHGVGDPEAALRLSNQLLAQGADVSLRSRWTNMNALHYAAYFDVPELIRILLKASKPKVLNSTCSDFCHGTALHIAASNLCLGAVKCLLEHGANPAVRNDKGQDPAEVVP 227          

HSP 2 Score: 163.696 bits (413), Expect = 1.422e-42
Identity = 84/208 (40.38%), Postives = 126/208 (60.58%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVT--PRNPLNPQTVVVDHGTVDISGVAA------KVDTGLNKSP----LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHIL---RSRHKNDEIIKATQQNRLSN 1661
            +G LR+CG  EFA G W G+ELDEP+GKN G+V G+ YF C  + G+FAPV++++K    VE+ P  VT  P+ P    + V      +            K  +G++  P    +++GD+VLVAGQ++G++RF G T+F+PG W+G+EL++P GK+DG+V GVRYF C    GVFAP SR+  +   +    +  ++K   Q  +S 
Sbjct:  295 TGTLRFCGTTEFASGQWVGLELDEPEGKNDGSVGGIRYFICSAKQGIFAPVSKITKA---VEQTPSSVTSTPKTPRMDLSRVTGKIKKEKKEKDREKTPRKKSLSGVSLDPDGVKVEVGDQVLVAGQKQGIVRFFGKTDFAPGYWFGVELEQPTGKHDGSVFGVRYFHCLPKYGVFAPPSRVQRIGGPKDPQGDGTLVKKVHQVTMSQ 499          

HSP 3 Score: 136.346 bits (342), Expect = 2.702e-33
Identity = 78/216 (36.11%), Postives = 113/216 (52.31%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL-CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKG 2036
            L++GDRV++   + G +RF G TEF+ G W G+ELD+P GKNDG+V G+RYF C A +G+FAP+S+            I KA +Q      SS+ +  +T ++  +     ++              T +  S +        V +  G QVL    + GI+R+ G  +F+ G W GVEL   TGKHDG V   RYF C P +G+   PSR+   G
Sbjct:  282 LKLGDRVVLDETKTGTLRFCGTTEFASGQWVGLELDEPEGKNDGSVGGIRYFICSAKQGIFAPVSK------------ITKAVEQTP----SSVTSTPKTPRMDLS----RVTGKIKKEKKEKDREKTPRKKSLSGVSLDPDGVKVEVGDQVLVAGQKQGIVRFFGKTDFAPGYWFGVELEQPTGKHDGSVFGVRYFHCLPKYGVFAPPSRVQRIG 477          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Zebrafish
Match: CABZ01072096.1 (pep chromosome:GRCz11:10:44408675:44411032:-1 gene:ENSDARG00000079483.4 transcript:ENSDART00000111509.4 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CABZ01072096.1)

HSP 1 Score: 146.362 bits (368), Expect = 1.128e-38
Identity = 73/183 (39.89%), Postives = 118/183 (64.48%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLN-----PQTVVVD-HGTVDISGVAAKVDTGLNKS--------PLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL 1592
            G++++ G  +FA G WAG+ LDEP GKN G+V+GV YFQC++ YG+F   +++++  ++  +     TP +  +     PQT  V+   + +++  A++  + L+++         L++GDRVLV G + GV+RF+G T+F+ G W G+ELD+P+GKNDGAV G RYFQC    G+FAP  ++
Sbjct:   60 GLVQFVGGTQFAPGQWAGIVLDEPIGKNDGSVSGVRYFQCKDLYGIFTRPSKLTRSPVQEREPNGTQTPADKTSEPAAAPQTTTVNTRNSSNLTRTASESASNLSETDSAKKIQRELKLGDRVLVGGSKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGGRYFQCLPKYGLFAPTHKV 242          

HSP 2 Score: 128.642 bits (322), Expect = 1.606e-32
Identity = 73/208 (35.10%), Postives = 106/208 (50.96%), Query Frame = 3
Query: 1386 SPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMV 2009
            S   +G+RV V G + G+++FVG T+F+PG W GI LD+P+GKNDG+V+GVRYFQC    G+F   S+L    +R    E      Q      S    A QT  ++  +S N    A       S   +  +       GD   RV       ++  ++ G++R++G  +F+ G W GVEL    GK+DG V   RYF C P +G+  
Sbjct:   44 SDFSVGERVWVNGNKPGLVQFVGGTQFAPGQWAGIVLDEPIGKNDGSVSGVRYFQCKDLYGIFTRPSKL----TRSPVQEREPNGTQTPADKTSEPAAAPQTTTVNTRNSSNLTRTASESASNLSETDSAKKIQRELKLGD---RV-------LVGGSKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGGRYFQCLPKYGLFA 237          

HSP 3 Score: 75.8702 bits (185), Expect = 1.757e-14
Identity = 31/59 (52.54%), Postives = 42/59 (71.19%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGI 1259
            +G++R+ G  +FA G W GVELDEP GKN G V G  YFQC  +YG+FAP  +V+++G 
Sbjct:  189 AGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGGRYFQCLPKYGLFAPTHKVTRIGF 247          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Zebrafish
Match: clip1a (CAP-GLY domain containing linker protein 1a [Source:ZFIN;Acc:ZDB-GENE-060526-282])

HSP 1 Score: 154.836 bits (390), Expect = 3.076e-38
Identity = 74/193 (38.34%), Postives = 109/193 (56.48%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVE------------------------KRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL 1592
            G++++ G  +FA G WAG+ LDEP GKN G+V+GV YFQCE   G+F   +++S    E +                        K+P   +P     P + +V   +  +S ++        +  L+I DRVLVAG + GV+RF+G T+F+ G W G+ELD+P+GKNDGAV G RYFQC    G+FAP+ ++
Sbjct:   66 GVVQFLGETQFAPGQWAGIVLDEPIGKNDGSVSGVRYFQCEALRGIFTRPSKLSSTEGEADGTTTAPPSRAASPTPSTTSVSASSKKP--ASPTKAAAPTSDLVRSKSESVSNLSESGSVKKGERELKINDRVLVAGSKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQPKYGLFAPVHKV 256          

HSP 2 Score: 133.265 bits (334), Expect = 2.475e-31
Identity = 81/224 (36.16%), Postives = 122/224 (54.46%), Query Frame = 3
Query: 1356 AAKVDTGLNKSP-----LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL-CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMV 2009
            AA+ D    ++P      ++GDRV V G + GV++F+G T+F+PG W GI LD+P+GKNDG+V+GVRYFQC A RG+F   S+L    +  + D    A      S   S  + S + +    +SP   +   S L+ S S S ++   S +      G   L+   +VL   ++ G++R++G  +F+ G W GVEL    GK+DG V   RYF C P +G+  
Sbjct:   35 AAQSDKSSAEAPDAGEQFKVGDRVWVNGNKPGVVQFLGETQFAPGQWAGIVLDEPIGKNDGSVSGVRYFQCEALRGIFTRPSKLS--STEGEADGTTTAPPSRAASPTPSTTSVSASSK--KPASPTKAAAPTSDLVRSKSESVSNLSESGSV---KKGERELKINDRVLVAGSKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQPKYGLFA 251          

HSP 3 Score: 85.5001 bits (210), Expect = 1.796e-16
Identity = 32/59 (54.24%), Postives = 44/59 (74.58%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGI 1259
            +G++R+ G  +FA G W GVELDEP GKN G V G  YFQC+ +YG+FAPV +V+++G 
Sbjct:  203 AGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQPKYGLFAPVHKVTRIGF 261          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Zebrafish
Match: zgc:103755 (zgc:103755 [Source:ZFIN;Acc:ZDB-GENE-041010-101])

HSP 1 Score: 142.51 bits (358), Expect = 6.799e-38
Identity = 91/250 (36.40%), Postives = 127/250 (50.80%), Query Frame = 3
Query:   51 MTSQDVLVMSVDTISERRKY---------PETHPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLL--DRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNMTTIRNNLGTL 761
            MT +DVL  S +  S R  Y         P  H    AP+  +C      FFD +   C +VL   + T+ +LFAVLRQW   VQ  +  I  ++ K G  VNDRDGLTDMT+LHY  K+GA   G+ E A      LL  GA    R +WT+M  LHYA YFDV P ++++L   +  ++ + C +   G  LHIAA+ L     R L++ GA+  F   R   P    P +  +  ++ T  + L T+
Sbjct:    1 MTKEDVLRESSEGDSVRGCYSFIPCNSDRPVIHQVASAPMPSDCE---FSFFDPNDPLCQEVLQDPQTTVPELFAVLRQWVPQVQRNISIIGNEILKRGCGVNDRDGLTDMTLLHYCCKAGAPGIGDAESAAGFAHQLLSLGAEPSLRSRWTNMNALHYAAYFDVPPLIQVVLQASQPGEVDSTCSDFDFGTALHIAASNLCTSAVRCLLELGANPAFKFLRSGCPSSRNPSLGNIHIHIQTHTHTLRTI 247          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Xenopus
Match: antxr1 (ANTXR cell adhesion molecule 1 [Source:Xenbase;Acc:XB-GENE-986530])

HSP 1 Score: 168.703 bits (426), Expect = 1.665e-44
Identity = 89/181 (49.17%), Postives = 115/181 (63.54%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDT--------GLNKSPLQI--GDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLH 1595
            +G LR+CG  EFA G W GVELDEP+GKN G+V G+ YF C  + G+FAPV+++SK     ++ P  VT      P+T  VD   V   G   K  T         L+K  L+I  GD+VLVAGQ++G +RF G T+F+PG W+GIEL+KP GK+DG+V GVRYF C    GVFAP SR+ 
Sbjct:  262 AGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGIRYFICPPKQGIFAPVSKISKA---PDQPPSSVTS----TPRTPRVDFSRVTGKGRKEKKATHKKSLSVGSLDKEGLKIDIGDQVLVAGQKQGFVRFYGKTDFAPGYWFGIELEKPTGKHDGSVFGVRYFTCSPKHGVFAPPSRVQ 435          

HSP 2 Score: 140.969 bits (354), Expect = 6.916e-35
Identity = 71/183 (38.80%), Postives = 107/183 (58.47%), Query Frame = 3
Query:  168 LCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLL--DRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLP 704
              FFD +   C ++L   + ++ +LFA++RQW   VQ +++ I  ++ K G +VNDRDGLTDMT+LHYA K+GA   G+   AVR+   LL  GA +  R +WT+M  LHYA YFDV   +  LL   +   + + C +   G  LHIAA+ L L   + L++ GA+    + + + P D +P
Sbjct:   12 FTFFDPNDPACQEILFDPQTSVPELFAIIRQWVPQVQHKIDIIGSEILKRGCHVNDRDGLTDMTLLHYACKAGAHGVGDPAAAVRLTNQLLLLGADITLRSRWTNMNALHYAAYFDVPELLRTLLKASKPKVLNSTCNDFNHGTALHIAASNLCLGAVKCLLEHGANPSVRNSKGQVPADVVP 194          

HSP 3 Score: 133.265 bits (334), Expect = 2.401e-32
Identity = 72/216 (33.33%), Postives = 111/216 (51.39%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL-CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKG 2036
            +++GDR+L+  ++ G +RF G TEF+ G W G+ELD+P GKNDG+V G+RYF C   +G+FAP+S+  I ++  +    + +T +    + S +    + ++ +                 S SV +  +       GD           QVL    + G +R+ G  +F+ G W G+EL   TGKHDG V   RYF C P HG+   PSR+   G
Sbjct:  249 MKLGDRILLDAEKAGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGIRYFICPPKQGIFAPVSK--ISKAPDQPPSSVTSTPRTPRVDFSRVTGKGRKEKKATHKK-------------SLSVGSLDKEGLKIDIGD-----------QVLVAGQKQGFVRFYGKTDFAPGYWFGIELEKPTGKHDGSVFGVRYFTCSPKHGVFAPPSRVQRTG 438          

HSP 4 Score: 68.5514 bits (166), Expect = 2.104e-11
Identity = 28/57 (49.12%), Postives = 40/57 (70.18%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVG 1256
            G +R+ G  +FA G W G+EL++P GK+ G+V GV YF C  ++GVFAP +RV + G
Sbjct:  382 GFVRFYGKTDFAPGYWFGIELEKPTGKHDGSVFGVRYFTCSPKHGVFAPPSRVQRTG 438          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Xenopus
Match: antxr1 (ANTXR cell adhesion molecule 1 [Source:Xenbase;Acc:XB-GENE-986530])

HSP 1 Score: 169.474 bits (428), Expect = 1.871e-44
Identity = 89/181 (49.17%), Postives = 115/181 (63.54%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDT--------GLNKSPLQI--GDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLH 1595
            +G LR+CG  EFA G W GVELDEP+GKN G+V G+ YF C  + G+FAPV+++SK     ++ P  VT      P+T  VD   V   G   K  T         L+K  L+I  GD+VLVAGQ++G +RF G T+F+PG W+GIEL+KP GK+DG+V GVRYF C    GVFAP SR+ 
Sbjct:  297 AGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGIRYFICPPKQGIFAPVSKISKA---PDQPPSSVTS----TPRTPRVDFSRVTGKGRKEKKATHKKSLSVGSLDKEGLKIDIGDQVLVAGQKQGFVRFYGKTDFAPGYWFGIELEKPTGKHDGSVFGVRYFTCSPKHGVFAPPSRVQ 470          

HSP 2 Score: 157.532 bits (397), Expect = 2.288e-40
Identity = 80/207 (38.65%), Postives = 121/207 (58.45%), Query Frame = 3
Query:   96 ERRKYPETHPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLL--DRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLP 704
            E+R+ P+ HP+  APL     +Y   FFD +   C ++L   + ++ +LFA++RQW   VQ +++ I  ++ K G +VNDRDGLTDMT+LHYA K+GA   G+   AVR+   LL  GA +  R +WT+M  LHYA YFDV   +  LL   +   + + C +   G  LHIAA+ L L   + L++ GA+    + + + P D +P
Sbjct:   26 EKRRRPKVHPSAPAPLP---KDYAFTFFDPNDPACQEILFDPQTSVPELFAIIRQWVPQVQHKIDIIGSEILKRGCHVNDRDGLTDMTLLHYACKAGAHGVGDPAAAVRLTNQLLLLGADITLRSRWTNMNALHYAAYFDVPELLRTLLKASKPKVLNSTCNDFNHGTALHIAASNLCLGAVKCLLEHGANPSVRNSKGQVPADVVP 229          

HSP 3 Score: 134.42 bits (337), Expect = 1.452e-32
Identity = 72/216 (33.33%), Postives = 112/216 (51.85%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL-CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKG 2036
            +++GDR+L+  ++ G +RF G TEF+ G W G+ELD+P GKNDG+V G+RYF C   +G+FAP+S+  I ++  +    + +T +    + S +    + ++                         TH+ +      D  G + +  G QVL    + G +R+ G  +F+ G W G+EL   TGKHDG V   RYF C P HG+   PSR+   G
Sbjct:  284 MKLGDRILLDAEKAGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGIRYFICPPKQGIFAPVSK--ISKAPDQPPSSVTSTPRTPRVDFSRVTGKGRKEK-----------------------KATHKKSLSVGSLDKEG-LKIDIGDQVLVAGQKQGFVRFYGKTDFAPGYWFGIELEKPTGKHDGSVFGVRYFTCSPKHGVFAPPSRVQRTG 473          

HSP 4 Score: 68.9366 bits (167), Expect = 1.767e-11
Identity = 28/57 (49.12%), Postives = 40/57 (70.18%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVG 1256
            G +R+ G  +FA G W G+EL++P GK+ G+V GV YF C  ++GVFAP +RV + G
Sbjct:  417 GFVRFYGKTDFAPGYWFGIELEKPTGKHDGSVFGVRYFTCSPKHGVFAPPSRVQRTG 473          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Xenopus
Match: antxr1 (ANTXR cell adhesion molecule 1 [Source:Xenbase;Acc:XB-GENE-986530])

HSP 1 Score: 168.703 bits (426), Expect = 3.147e-44
Identity = 89/181 (49.17%), Postives = 115/181 (63.54%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDT--------GLNKSPLQI--GDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLH 1595
            +G LR+CG  EFA G W GVELDEP+GKN G+V G+ YF C  + G+FAPV+++SK     ++ P  VT      P+T  VD   V   G   K  T         L+K  L+I  GD+VLVAGQ++G +RF G T+F+PG W+GIEL+KP GK+DG+V GVRYF C    GVFAP SR+ 
Sbjct:  294 AGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGIRYFICPPKQGIFAPVSKISKA---PDQPPSSVTS----TPRTPRVDFSRVTGKGRKEKKATHKKSLSVGSLDKEGLKIDIGDQVLVAGQKQGFVRFYGKTDFAPGYWFGIELEKPTGKHDGSVFGVRYFTCSPKHGVFAPPSRVQ 467          

HSP 2 Score: 140.969 bits (354), Expect = 8.861e-35
Identity = 71/184 (38.59%), Postives = 107/184 (58.15%), Query Frame = 3
Query:  165 GLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLL--DRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLP 704
               FFD +   C ++L   + ++ +LFA++RQW   VQ +++ I  ++ K G +VNDRDGLTDMT+LHYA K+GA   G+   AVR+   LL  GA +  R +WT+M  LHYA YFDV   +  LL   +   + + C +   G  LHIAA+ L L   + L++ GA+    + + + P D +P
Sbjct:   43 AFTFFDPNDPACQEILFDPQTSVPELFAIIRQWVPQVQHKIDIIGSEILKRGCHVNDRDGLTDMTLLHYACKAGAHGVGDPAAAVRLTNQLLLLGADITLRSRWTNMNALHYAAYFDVPELLRTLLKASKPKVLNSTCNDFNHGTALHIAASNLCLGAVKCLLEHGANPSVRNSKGQVPADVVP 226          

HSP 3 Score: 133.265 bits (334), Expect = 3.559e-32
Identity = 72/216 (33.33%), Postives = 111/216 (51.39%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL-CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKG 2036
            +++GDR+L+  ++ G +RF G TEF+ G W G+ELD+P GKNDG+V G+RYF C   +G+FAP+S+  I ++  +    + +T +    + S +    + ++ +                 S SV +  +       GD           QVL    + G +R+ G  +F+ G W G+EL   TGKHDG V   RYF C P HG+   PSR+   G
Sbjct:  281 MKLGDRILLDAEKAGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGIRYFICPPKQGIFAPVSK--ISKAPDQPPSSVTSTPRTPRVDFSRVTGKGRKEKKATHKK-------------SLSVGSLDKEGLKIDIGD-----------QVLVAGQKQGFVRFYGKTDFAPGYWFGIELEKPTGKHDGSVFGVRYFTCSPKHGVFAPPSRVQRTG 470          

HSP 4 Score: 68.5514 bits (166), Expect = 2.443e-11
Identity = 28/57 (49.12%), Postives = 40/57 (70.18%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVG 1256
            G +R+ G  +FA G W G+EL++P GK+ G+V GV YF C  ++GVFAP +RV + G
Sbjct:  414 GFVRFYGKTDFAPGYWFGIELEKPTGKHDGSVFGVRYFTCSPKHGVFAPPSRVQRTG 470          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Xenopus
Match: antxr1 (ANTXR cell adhesion molecule 1 [Source:Xenbase;Acc:XB-GENE-986530])

HSP 1 Score: 167.933 bits (424), Expect = 4.776e-44
Identity = 88/177 (49.72%), Postives = 114/177 (64.41%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDT----GLNKSPLQI--GDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLH 1595
            +G LR+CG  EFA G W GVELDEP+GKN G+V G+ YF C  + G+FAPV+++SK     ++ P  VT      P+T  VD   V   G   K        L+K  L+I  GD+VLVAGQ++G +RF G T+F+PG W+GIEL+KP GK+DG+V GVRYF C    GVFAP SR+ 
Sbjct:  294 AGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGIRYFICPPKQGIFAPVSKISKA---PDQPPSSVTS----TPRTPRVDFSRVTGKGRKEKKACPSVGSLDKEGLKIDIGDQVLVAGQKQGFVRFYGKTDFAPGYWFGIELEKPTGKHDGSVFGVRYFTCSPKHGVFAPPSRVQ 463          

HSP 2 Score: 140.969 bits (354), Expect = 8.137e-35
Identity = 71/184 (38.59%), Postives = 107/184 (58.15%), Query Frame = 3
Query:  165 GLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLL--DRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLP 704
               FFD +   C ++L   + ++ +LFA++RQW   VQ +++ I  ++ K G +VNDRDGLTDMT+LHYA K+GA   G+   AVR+   LL  GA +  R +WT+M  LHYA YFDV   +  LL   +   + + C +   G  LHIAA+ L L   + L++ GA+    + + + P D +P
Sbjct:   43 AFTFFDPNDPACQEILFDPQTSVPELFAIIRQWVPQVQHKIDIIGSEILKRGCHVNDRDGLTDMTLLHYACKAGAHGVGDPAAAVRLTNQLLLLGADITLRSRWTNMNALHYAAYFDVPELLRTLLKASKPKVLNSTCNDFNHGTALHIAASNLCLGAVKCLLEHGANPSVRNSKGQVPADVVP 226          

HSP 3 Score: 131.724 bits (330), Expect = 9.547e-32
Identity = 77/216 (35.65%), Postives = 106/216 (49.07%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL-CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKG 2036
            +++GDR+L+  ++ G +RF G TEF+ G W G+ELD+P GKNDG+V G+RYF C   +G+FAP+S+            I KA  Q   S  S+  T       S  +          P     SV +  +       GD           QVL    + G +R+ G  +F+ G W G+EL   TGKHDG V   RYF C P HG+   PSR+   G
Sbjct:  281 MKLGDRILLDAEKAGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGIRYFICPPKQGIFAPVSK------------ISKAPDQPPSSVTSTPRTPRV--DFSRVTGKGRKEKKACP-----SVGSLDKEGLKIDIGD-----------QVLVAGQKQGFVRFYGKTDFAPGYWFGIELEKPTGKHDGSVFGVRYFTCSPKHGVFAPPSRVQRTG 466          

HSP 4 Score: 68.1662 bits (165), Expect = 2.575e-11
Identity = 28/57 (49.12%), Postives = 40/57 (70.18%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVG 1256
            G +R+ G  +FA G W G+EL++P GK+ G+V GV YF C  ++GVFAP +RV + G
Sbjct:  410 GFVRFYGKTDFAPGYWFGIELEKPTGKHDGSVFGVRYFTCSPKHGVFAPPSRVQRTG 466          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Xenopus
Match: CLIP4 (CAP-GLY domain containing linker protein family, member 4 [Source:NCBI gene;Acc:100495216])

HSP 1 Score: 162.925 bits (411), Expect = 1.904e-42
Identity = 85/213 (39.91%), Postives = 119/213 (55.87%), Query Frame = 3
Query:  105 KYPETHPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLL--DRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNM 731
            K P T     AP+  +C      FFD +   C  +L     ++ +LFA+LRQW   VQ  ++ I  ++ K G  VNDRDGLTDMT+LHY  KSGAQ  G+ E A    + L+D GA +  R +WT+M  LHYA YFDV   + ++L   +  D+ A C +   G  LHIAA+ L L   R L++ GA+  F DD+ + P D +P   ++P  M
Sbjct:   30 KRPVTFSICAAPMPSDCE---FSFFDPNDASCQDILFDPNTSVPELFAILRQWVPQVQQSIDVIGNEILKRGCGVNDRDGLTDMTLLHYTCKSGAQGIGDAETAATFASRLIDLGADINLRSRWTNMNALHYAAYFDVPELIGVILKNSKPKDVDATCSDFDFGTALHIAASNLCLGAVRSLLEHGANPAFRDDKGQVPADVVPDPVDMPLEM 239          

HSP 2 Score: 94.7449 bits (234), Expect = 8.990e-20
Identity = 40/74 (54.05%), Postives = 55/74 (74.32%), Query Frame = 3
Query: 1371 TGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL 1592
            + L  + L+IGDRVL+AGQ+ G +RF G TEF+ G W GIE+D+  GKNDG++ GV YF+C    G+FAPLS++
Sbjct:  278 SSLLAAALKIGDRVLIAGQKVGTLRFCGSTEFASGQWAGIEMDESEGKNDGSIGGVTYFKCPQKHGIFAPLSKI 351          

HSP 3 Score: 82.0333 bits (201), Expect = 1.024e-15
Identity = 29/56 (51.79%), Postives = 45/56 (80.36%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKV 1253
            G LR+CG  EFA G WAG+E+DE +GKN G++ GV YF+C +++G+FAP++++S+ 
Sbjct:  299 GTLRFCGSTEFASGQWAGIEMDESEGKNDGSIGGVTYFKCPQKHGIFAPLSKISRA 354          

HSP 4 Score: 56.6102 bits (135), Expect = 1.197e-7
Identity = 26/68 (38.24%), Postives = 39/68 (57.35%), Query Frame = 3
Query: 1827 LREGLQVL-CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRIS 2027
            L+ G +VL    ++G +R+ G+  F+ G W G+E+    GK+DG +    YF CP  HGI    S+IS
Sbjct:  285 LKIGDRVLIAGQKVGTLRFCGSTEFASGQWAGIEMDESEGKNDGSIGGVTYFKCPQKHGIFAPLSKIS 352          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Mouse
Match: Clip4 (CAP-GLY domain containing linker protein family, member 4 [Source:MGI Symbol;Acc:MGI:1919100])

HSP 1 Score: 188.734 bits (478), Expect = 2.602e-50
Identity = 105/245 (42.86%), Postives = 143/245 (58.37%), Query Frame = 3
Query: 1347 SGVAAKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHS------SMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL--CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLI 2057
            S   +  +   ++  L +G+RVLV GQR G I+F G T F+PG WYGIEL+KP GKNDG+V GV+YF C    G+FAP SR+  L         I + +Q    NHS      S  T S + Q    +  N  +  ++ L  S S STT         G   G V L EG QVL   +NE+  +RYVG  +F+ GIW+G+ELR   GK+DG V +KRYF C P++G++VRPSR++ +GI+G+ LI
Sbjct:  469 SSATSAANNSHHEGALHLGERVLVVGQRVGTIKFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRVQRLSDSLDTLSEISSNKQ----NHSYPGFRRSFSTTSASSQ-KEINRRNAFAKTKTTLRRSWSSSTTA--------GGLEGTVKLHEGSQVLLTSSNEMATVRYVGPTDFASGIWLGLELRSAKGKNDGAVGDKRYFTCKPNYGVLVRPSRVTYRGISGSKLI 700          

HSP 2 Score: 159.073 bits (401), Expect = 3.370e-40
Identity = 80/203 (39.41%), Postives = 117/203 (57.64%), Query Frame = 3
Query:  135 APLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLL--DRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNM 731
            AP+  +C      FFD +   C ++L   + ++ +LFA+LRQW   VQ  ++ I  ++ K G NVNDRDGLTDMT+LHY  KSGA   G+ E AV+  A L+D GA    R +WT+M  LHYA YFDV   + ++L   +  D+ A C +   G  LHIAA  L     + L++ GA+  F +D+ + P + +P   ++P  M
Sbjct:   38 APMPSDCE---FSFFDPNDASCQEILFDPKTSVSELFAILRQWVPQVQQNIDIIGNEILKRGCNVNDRDGLTDMTLLHYTCKSGAHGIGDIETAVKFAAQLIDLGADASLRSRWTNMNALHYASYFDVPELIRVILKTSKPKDVDATCSDFNFGTALHIAAHNLCAGAVKTLLELGANPAFRNDKGQIPAEVVPDPVDMPLEM 237          

HSP 3 Score: 125.176 bits (313), Expect = 3.606e-29
Identity = 89/313 (28.43%), Postives = 142/313 (45.37%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRI 2024
            G LR+CG  EFA G WAG+ELDEP+GKN+G+V  V YF+C  +YG+FAP++++SK+     K  R  T   P    T       VD++   ++V++GL  S  +      +          + P+E  P         K V +ND A  G                                 ++  +   ++   +T S   + + + SP+  S A + L +S++ +  +  +         G + L E + V+    +G I++ G  NF+ G W G+EL    GK+DG V   +YF C P +GI   PSR+
Sbjct:  297 GTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGRVQYFKCAPKYGIFAPLSKISKL-----KDGRKTTTHTPSTRATPHARSQKVDVAHFTSRVNSGLTTSKKETASESTLT---------LPPSE-EP---------KTVAENDAAQPGSMS--------------------------SSSSSSSLDHKQSYPKKLTTSSGGKKTLSKSPSLPSRASAGLKSSATSAANNSHH--------EGALHLGERVLVV-GQRVGTIKFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRV 550          

HSP 4 Score: 114.39 bits (285), Expect = 8.922e-26
Identity = 66/192 (34.38%), Postives = 94/192 (48.96%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPR-NPLNP-------QTVVVDHGTVDISGVAAKVDTGLNKS--------------PLQIGDRVLVAGQRK-GVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL 1592
            G +++ G   FA G W G+EL++P GKN G+V GV YF C  +YG+FAP +RV ++   ++      + + N   P        T       ++     AK  T L +S               L  G +VL+    +   +R+VGPT+F+ G W G+EL    GKNDGAV   RYF C  N GV    SR+
Sbjct:  498 GTIKFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRVQRLSDSLDTLSEISSNKQNHSYPGFRRSFSTTSASSQKEINRRNAFAKTKTTLRRSWSSSTTAGGLEGTVKLHEGSQVLLTSSNEMATVRYVGPTDFASGIWLGLELRSAKGKNDGAVGDKRYFTCKPNYGVLVRPSRV 689          

HSP 5 Score: 62.003 bits (149), Expect = 2.439e-9
Identity = 45/163 (27.61%), Postives = 70/163 (42.94%), Query Frame = 3
Query:  819 PETGVLSPPIRRQTPGPFPPSHLDLVSISDENEIYEAP---TPVSRPVSMWRDEIDNRNLASPSRVSAKVT---------LQAMXXXXXXXXXXXXXXXXSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEK 1271
            P  G+ +PP R Q       S   L  IS   + +  P      S   +  + EI+ RN  + ++ + + +         L+    L     ++    +    +RY GP +FA G+W G+EL   +GKN G V    YF C+  YGV    +RV+  GI   K
Sbjct:  539 PRYGIFAPPSRVQR---LSDSLDTLSEISSNKQNHSYPGFRRSFSTTSASSQKEINRRNAFAKTKTTLRRSWSSSTTAGGLEGTVKLHEGSQVLLTSSNEMATVRYVGPTDFASGIWLGLELRSAKGKNDGAVGDKRYFTCKPNYGVLVRPSRVTYRGISGSK 698          

HSP 6 Score: 55.0694 bits (131), Expect = 3.078e-7
Identity = 23/61 (37.70%), Postives = 35/61 (57.38%), Query Frame = 3
Query: 1845 VLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRIS 2027
            V+   ++G +R+ G   F+ G W G+EL    GK++G V   +YF C P +GI    S+IS
Sbjct:  290 VIAGQKVGTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGRVQYFKCAPKYGIFAPLSKIS 350          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Mouse
Match: Clip3 (CAP-GLY domain containing linker protein 3 [Source:MGI Symbol;Acc:MGI:1923936])

HSP 1 Score: 167.162 bits (422), Expect = 1.089e-43
Identity = 88/180 (48.89%), Postives = 112/180 (62.22%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVT--PRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQ-------IGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLH 1595
            +G LR+CG  EFA G W GVELDEP+GKN G+V GV YF C  + G+FA V++VSK    V+  P  VT  PR P    + V   G  +  G      +    S  Q       +GD+VLVAGQ++G++RF G T+F+PG WYGIELD+P GK+DG+V GVRYF C    GVFAP SR+ 
Sbjct:  307 TGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKQGLFASVSKVSKA---VDAPPSSVTSTPRTPRMDFSRVTGKGRREHKGKKKSPSSPSLGSLQQREGAKAEVGDQVLVAGQKQGIVRFYGKTDFAPGYWYGIELDQPTGKHDGSVFGVRYFTCAPRHGVFAPASRIQ 483          

HSP 2 Score: 161.77 bits (408), Expect = 8.737e-42
Identity = 86/208 (41.35%), Postives = 125/208 (60.10%), Query Frame = 3
Query:   96 ERRKYPETHPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLLDRGADIRAI---CRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLP 704
            ERR+ P  HP+  APL     +Y   FFD +   C ++L   + TI +LFA++RQW   VQ +++ I  ++ + G +VNDRDGLTDMT+LHYA K+GA   G+   AVR+   LL  GA +  R +WT+M  LHYA YFDV   V +LL +GA  R +   C +   G+ LHIAA+ L L  A+ L++ GA+    + + + P + +P
Sbjct:   36 ERRQKPVVHPSAPAPLPK---DYAFTFFDPNDPACQEILFDPKTTIPELFAIVRQWVPQVQHKIDVIGNEILRRGCHVNDRDGLTDMTLLHYACKAGAHGVGDPAAAVRLSQQLLALGADVTLRSRWTNMNALHYAAYFDVPDLVRVLL-KGARPRVVNSTCSDFNHGSALHIAASNLCLGAAKCLLEHGANPALRNRKGQVPAEVVP 239          

HSP 3 Score: 129.798 bits (325), Expect = 3.928e-31
Identity = 78/226 (34.51%), Postives = 111/226 (49.12%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTT----HQFNSPTYFGDSSGRVFLREGLQ-------VLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKG 2036
            L++GDRVL+ GQ+ G +RF G TEF+ G W G+ELD+P GKNDG+V GVRYF C   +G+FA +S++                                  +  +A   +  S  ++P M  S V+      H+    +    S G +  REG +       ++   + GI+R+ G  +F+ G W G+EL   TGKHDG V   RYF C P HG+    SRI   G
Sbjct:  294 LRLGDRVLLDGQKTGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKQGLFASVSKV---------------------------------SKAVDAPPSSVTSTPRTPRMDFSRVTGKGRREHKGKKKSPSSPSLGSLQQREGAKAEVGDQVLVAGQKQGIVRFYGKTDFAPGYWYGIELDQPTGKHDGSVFGVRYFTCAPRHGVFAPASRIQRIG 486          

HSP 4 Score: 73.1738 bits (178), Expect = 5.375e-13
Identity = 29/57 (50.88%), Postives = 42/57 (73.68%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVG 1256
            GI+R+ G  +FA G W G+ELD+P GK+ G+V GV YF C  ++GVFAP +R+ ++G
Sbjct:  430 GIVRFYGKTDFAPGYWYGIELDQPTGKHDGSVFGVRYFTCAPRHGVFAPASRIQRIG 486          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Mouse
Match: Clip4 (CAP-GLY domain containing linker protein family, member 4 [Source:MGI Symbol;Acc:MGI:1919100])

HSP 1 Score: 153.68 bits (387), Expect = 3.472e-41
Identity = 80/203 (39.41%), Postives = 117/203 (57.64%), Query Frame = 3
Query:  135 APLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLL--DRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNM 731
            AP+  +C      FFD +   C ++L   + ++ +LFA+LRQW   VQ  ++ I  ++ K G NVNDRDGLTDMT+LHY  KSGA   G+ E AV+  A L+D GA    R +WT+M  LHYA YFDV   + ++L   +  D+ A C +   G  LHIAA  L     + L++ GA+  F +D+ + P + +P   ++P  M
Sbjct:   38 APMPSDCE---FSFFDPNDASCQEILFDPKTSVSELFAILRQWVPQVQQNIDIIGNEILKRGCNVNDRDGLTDMTLLHYTCKSGAHGIGDIETAVKFAAQLIDLGADASLRSRWTNMNALHYASYFDVPELIRVILKTSKPKDVDATCSDFNFGTALHIAAHNLCAGAVKTLLELGANPAFRNDKGQIPAEVVPDPVDMPLEM 237          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Mouse
Match: Clip4 (CAP-GLY domain containing linker protein family, member 4 [Source:MGI Symbol;Acc:MGI:1919100])

HSP 1 Score: 155.992 bits (393), Expect = 6.131e-41
Identity = 80/203 (39.41%), Postives = 117/203 (57.64%), Query Frame = 3
Query:  135 APLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLL--DRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNM 731
            AP+  +C      FFD +   C ++L   + ++ +LFA+LRQW   VQ  ++ I  ++ K G NVNDRDGLTDMT+LHY  KSGA   G+ E AV+  A L+D GA    R +WT+M  LHYA YFDV   + ++L   +  D+ A C +   G  LHIAA  L     + L++ GA+  F +D+ + P + +P   ++P  M
Sbjct:   38 APMPSDCE---FSFFDPNDASCQEILFDPKTSVSELFAILRQWVPQVQQNIDIIGNEILKRGCNVNDRDGLTDMTLLHYTCKSGAHGIGDIETAVKFAAQLIDLGADASLRSRWTNMNALHYASYFDVPELIRVILKTSKPKDVDATCSDFNFGTALHIAAHNLCAGAVKTLLELGANPAFRNDKGQIPAEVVPDPVDMPLEM 237          

HSP 2 Score: 95.1301 bits (235), Expect = 2.761e-20
Identity = 40/75 (53.33%), Postives = 56/75 (74.67%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHK 1616
            L++GDRV++AGQ+ G +RF G TEF+ G W GIELD+P GKN+G+V  V+YF+C    G+FAPLS++  L+   K
Sbjct:  283 LKLGDRVVIAGQKVGTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGRVQYFKCAPKYGIFAPLSKISKLKDGRK 357          

HSP 3 Score: 90.1225 bits (222), Expect = 1.222e-18
Identity = 42/97 (43.30%), Postives = 61/97 (62.89%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTG 1376
            G LR+CG  EFA G WAG+ELDEP+GKN+G+V  V YF+C  +YG+FAP++++SK+     K  R  T   P    T       VD++   ++V++G
Sbjct:  297 GTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGRVQYFKCAPKYGIFAPLSKISKL-----KDGRKTTTHTPSTRATPHARSQKVDVAHFTSRVNSG 388          

HSP 4 Score: 54.299 bits (129), Expect = 3.750e-7
Identity = 23/61 (37.70%), Postives = 35/61 (57.38%), Query Frame = 3
Query: 1845 VLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRIS 2027
            V+   ++G +R+ G   F+ G W G+EL    GK++G V   +YF C P +GI    S+IS
Sbjct:  290 VIAGQKVGTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGRVQYFKCAPKYGIFAPLSKIS 350          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Mouse
Match: Clip4 (CAP-GLY domain containing linker protein family, member 4 [Source:MGI Symbol;Acc:MGI:1919100])

HSP 1 Score: 158.688 bits (400), Expect = 8.692e-41
Identity = 80/203 (39.41%), Postives = 117/203 (57.64%), Query Frame = 3
Query:  135 APLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLL--DRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNM 731
            AP+  +C      FFD +   C ++L   + ++ +LFA+LRQW   VQ  ++ I  ++ K G NVNDRDGLTDMT+LHY  KSGA   G+ E AV+  A L+D GA    R +WT+M  LHYA YFDV   + ++L   +  D+ A C +   G  LHIAA  L     + L++ GA+  F +D+ + P + +P   ++P  M
Sbjct:   38 APMPSDCE---FSFFDPNDASCQEILFDPKTSVSELFAILRQWVPQVQQNIDIIGNEILKRGCNVNDRDGLTDMTLLHYTCKSGAHGIGDIETAVKFAAQLIDLGADASLRSRWTNMNALHYASYFDVPELIRVILKTSKPKDVDATCSDFNFGTALHIAAHNLCAGAVKTLLELGANPAFRNDKGQIPAEVVPDPVDMPLEM 237          

HSP 2 Score: 149.443 bits (376), Expect = 1.134e-37
Identity = 85/226 (37.61%), Postives = 115/226 (50.88%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRV---SKVGIEVEKRPRCVTPRNPLNP-------QTVVVDH-------------GTVDIS-----------GVAAKVDTGLNKS----PLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQN 1649
            G LR+CG  EFA G WAG+ELDEP+GKN+G+V  V YF+C  +YG+          S + +   + P+ V   +   P        +  +DH             G   +S           G+ +   +  N S     L +G+RVLV GQR G I+F G T F+PG WYGIEL+KP GKNDG+V GV+YF C    G+FAP SR+  L         I + +QN
Sbjct:  297 GTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGRVQYFKCAPKYGLTTSKKETASESTLTLPPSEEPKTVAENDAAQPGSMSSSSSSSSLDHKQSYPKKLTTSSGGKKTLSKSPSLPSRASAGLKSSATSAANNSHHEGALHLGERVLVVGQRVGTIKFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRVQRLSDSLDTLSEISSNKQN 522          

HSP 3 Score: 119.013 bits (297), Expect = 1.448e-27
Identity = 75/230 (32.61%), Postives = 119/230 (51.74%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGV------FAPLSRLHILRSRH-----KNDEI--------IKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRI 2024
            L++GDRV++AGQ+ G +RF G TEF+ G W GIELD+P GKN+G+V  V+YF+C    G+       A  S L +  S       +ND            ++  +   ++   +T S   + + + SP+  S A + L +S++ +  +  +         G + L E + V+    +G I++ G  NF+ G W G+EL    GK+DG V   +YF C P +GI   PSR+
Sbjct:  283 LKLGDRVVIAGQKVGTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGRVQYFKCAPKYGLTTSKKETASESTLTLPPSEEPKTVAENDAAQPGSMSSSSSSSSLDHKQSYPKKLTTSSGGKKTLSKSPSLPSRASAGLKSSATSAANNSHH--------EGALHLGERVLVV-GQRVGTIKFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRV 503          
BLAST of CAP-Gly domain-containing linker protein vs. UniProt/SwissProt
Match: sp|Q8N3C7|CLIP4_HUMAN (CAP-Gly domain-containing linker protein 4 OS=Homo sapiens OX=9606 GN=CLIP4 PE=1 SV=1)

HSP 1 Score: 191.815 bits (486), Expect = 1.716e-50
Identity = 109/255 (42.75%), Postives = 148/255 (58.04%), Query Frame = 3
Query: 1353 VAAKVDTGLNKSP------------LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHS------SMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL--CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLI 2057
            ++++   GLN S             L++G+RVLV GQR G IRF G T F+PG WYGIEL+KP GKNDG+V GV+YF C    G+FAP SR+  +         I + +Q    NHS      S  T S + Q    +  N  S +++ L  S S + T         G   G V L EG QVL   +NE+G +RYVG  +F+ GIW+G+ELR   GK+DG V +KRYF C P+HG++VRPSR++ +GING+ L+
Sbjct:  460 LSSRASAGLNSSATSTANNSRCEGELRLGERVLVVGQRLGTIRFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRVQRVTDSLDTLSEISSNKQ----NHSYPGFRRSFSTTSASSQ-KEINRRNAFSKSKAALRRSWSSTPTA--------GGIEGSVKLHEGSQVLLTSSNEMGTVRYVGPTDFASGIWLGLELRSAKGKNDGSVGDKRYFTCKPNHGVLVRPSRVTYRGINGSKLV 701          

HSP 2 Score: 166.777 bits (421), Expect = 5.964e-42
Identity = 182/646 (28.17%), Postives = 262/646 (40.56%), Query Frame = 3
Query:  135 APLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQGEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYF------DVAPAVEL---LLDRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNMTTIRNNLGTLLCPTSPPGHTYYNANHRKPPETGVLSPPIRRQTPGPFPPSHLDLVSISDENEIYEAPTPVSRPVSMWRD------EIDNRNLASPSRVSAKVTLQAMXXXXXXXXXXXXXXXXSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL-CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRI 2024
            AP+  +C      FFD +   C ++L   + ++ +LFA+LRQW   VQ  ++ I  ++ K G NVNDRDGLT                                         DMT LHY          DV  AV+    L+D GADI    R     N LH AA     +  RV++           +   P D     S+   N  T  +     LC  +        AN     + G        Q P    P  +D+       E+ +A         M  D       I    L +   V+ K  L ++GL LGDRV++ G     G LR+CG  EFA G WAG+ELDEP+GKN+G+V  V YF+C  +YG+FAP++++SK     + R + +T          ++    +D++ V +KV+TGL  S         ++                PG     E  K V + D A+            G  +  S    L  R    +     +QN +S++   M+ S +           LS   S  + SS+ ST +         +S     LR G +VL     LG IR+ G  NF+ G W G+EL    GK+DG V   +YF C P +GI   PSR+
Sbjct:   38 APMPSDCE---FSFFDPNDASCQEILFDPKTSVSELFAILRQWVPQVQQNIDIIGNEILKRGCNVNDRDGLT-----------------------------------------DMTLLHYTCKSGAHGIGDVETAVKFATQLIDLGADISLRSR-WTNMNALHYAAYFDVPELIRVIL-----------KTSKPKDVDATCSDF--NFGTALHIAAYNLCAGAVKCLLEQGANPAFRNDKG--------QIPADVVPDPVDM-----PLEMADAAATAKEIKQMLLDAVPLSCNISKAMLPNYDHVTGKAMLTSLGLKLGDRVVIAGQKV--GTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGKVQYFKCAPKYGIFAPLSKISK----AKGRRKNITHTPSTKAAVPLIRSQKIDVAHVTSKVNTGLMTSKKDSASESTLS--------------LPPG-----EELKTVTEKDVAL-----------LGSVSSCSSTSSLEHRQSYPK-----KQNAISSNKKTMSKSPS-----------LSSRASAGLNSSATSTAN---------NSRCEGELRLGERVLVVGQRLGTIRFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRV 551          

HSP 3 Score: 114.39 bits (285), Expect = 6.953e-25
Identity = 66/192 (34.38%), Postives = 94/192 (48.96%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPR-NPLNP-------QTVVVDHGTVDISGVAAKVDTGLNKS--------------PLQIGDRVLVAGQRK-GVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL 1592
            G +R+ G   FA G W G+EL++P GKN G+V GV YF C  +YG+FAP +RV +V   ++      + + N   P        T       ++     +K    L +S               L  G +VL+    + G +R+VGPT+F+ G W G+EL    GKNDG+V   RYF C  N GV    SR+
Sbjct:  499 GTIRFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRVQRVTDSLDTLSEISSNKQNHSYPGFRRSFSTTSASSQKEINRRNAFSKSKAALRRSWSSTPTAGGIEGSVKLHEGSQVLLTSSNEMGTVRYVGPTDFASGIWLGLELRSAKGKNDGSVGDKRYFTCKPNHGVLVRPSRV 690          

HSP 4 Score: 62.7734 bits (151), Expect = 8.090e-9
Identity = 47/164 (28.66%), Postives = 74/164 (45.12%), Query Frame = 3
Query:  819 PETGVLSPPIRRQTPGPFPPSHLDLVS-ISDENEIYEAP---TPVSRPVSMWRDEIDNRNLASPSRVSAKVT---------LQAMXXXXXXXXXXXXXXXXSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEK 1271
            P  G+ +PP R Q         LD +S IS   + +  P      S   +  + EI+ RN  S S+ + + +         ++    L     ++    +  G +RY GP +FA G+W G+EL   +GKN G+V    YF C+  +GV    +RV+  GI   K
Sbjct:  540 PRYGIFAPPSRVQRV----TDSLDTLSEISSNKQNHSYPGFRRSFSTTSASSQKEINRRNAFSKSKAALRRSWSSTPTAGGIEGSVKLHEGSQVLLTSSNEMGTVRYVGPTDFASGIWLGLELRSAKGKNDGSVGDKRYFTCKPNHGVLVRPSRVTYRGINGSK 699          

HSP 5 Score: 55.0694 bits (131), Expect = 2.288e-6
Identity = 23/61 (37.70%), Postives = 35/61 (57.38%), Query Frame = 3
Query: 1845 VLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRIS 2027
            V+   ++G +R+ G   F+ G W G+EL    GK++G V   +YF C P +GI    S+IS
Sbjct:  290 VIAGQKVGTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGKVQYFKCAPKYGIFAPLSKIS 350          
BLAST of CAP-Gly domain-containing linker protein vs. UniProt/SwissProt
Match: sp|Q8CI96|CLIP4_MOUSE (CAP-Gly domain-containing linker protein 4 OS=Mus musculus OX=10090 GN=Clip4 PE=1 SV=1)

HSP 1 Score: 188.734 bits (478), Expect = 1.820e-49
Identity = 105/245 (42.86%), Postives = 143/245 (58.37%), Query Frame = 3
Query: 1347 SGVAAKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHS------SMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL--CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLI 2057
            S   +  +   ++  L +G+RVLV GQR G I+F G T F+PG WYGIEL+KP GKNDG+V GV+YF C    G+FAP SR+  L         I + +Q    NHS      S  T S + Q    +  N  +  ++ L  S S STT         G   G V L EG QVL   +NE+  +RYVG  +F+ GIW+G+ELR   GK+DG V +KRYF C P++G++VRPSR++ +GI+G+ LI
Sbjct:  469 SSATSAANNSHHEGALHLGERVLVVGQRVGTIKFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRVQRLSDSLDTLSEISSNKQ----NHSYPGFRRSFSTTSASSQ-KEINRRNAFAKTKTTLRRSWSSSTTA--------GGLEGTVKLHEGSQVLLTSSNEMATVRYVGPTDFASGIWLGLELRSAKGKNDGAVGDKRYFTCKPNYGVLVRPSRVTYRGISGSKLI 700          

HSP 2 Score: 159.073 bits (401), Expect = 2.357e-39
Identity = 80/203 (39.41%), Postives = 117/203 (57.64%), Query Frame = 3
Query:  135 APLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLL--DRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNM 731
            AP+  +C      FFD +   C ++L   + ++ +LFA+LRQW   VQ  ++ I  ++ K G NVNDRDGLTDMT+LHY  KSGA   G+ E AV+  A L+D GA    R +WT+M  LHYA YFDV   + ++L   +  D+ A C +   G  LHIAA  L     + L++ GA+  F +D+ + P + +P   ++P  M
Sbjct:   38 APMPSDCE---FSFFDPNDASCQEILFDPKTSVSELFAILRQWVPQVQQNIDIIGNEILKRGCNVNDRDGLTDMTLLHYTCKSGAHGIGDIETAVKFAAQLIDLGADASLRSRWTNMNALHYASYFDVPELIRVILKTSKPKDVDATCSDFNFGTALHIAAHNLCAGAVKTLLELGANPAFRNDKGQIPAEVVPDPVDMPLEM 237          

HSP 3 Score: 125.176 bits (313), Expect = 2.522e-28
Identity = 89/313 (28.43%), Postives = 142/313 (45.37%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRI 2024
            G LR+CG  EFA G WAG+ELDEP+GKN+G+V  V YF+C  +YG+FAP++++SK+     K  R  T   P    T       VD++   ++V++GL  S  +      +          + P+E  P         K V +ND A  G                                 ++  +   ++   +T S   + + + SP+  S A + L +S++ +  +  +         G + L E + V+    +G I++ G  NF+ G W G+EL    GK+DG V   +YF C P +GI   PSR+
Sbjct:  297 GTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGRVQYFKCAPKYGIFAPLSKISKL-----KDGRKTTTHTPSTRATPHARSQKVDVAHFTSRVNSGLTTSKKETASESTLT---------LPPSE-EP---------KTVAENDAAQPGSMS--------------------------SSSSSSSLDHKQSYPKKLTTSSGGKKTLSKSPSLPSRASAGLKSSATSAANNSHH--------EGALHLGERVLVV-GQRVGTIKFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRV 550          

HSP 4 Score: 114.39 bits (285), Expect = 6.241e-25
Identity = 66/192 (34.38%), Postives = 94/192 (48.96%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPR-NPLNP-------QTVVVDHGTVDISGVAAKVDTGLNKS--------------PLQIGDRVLVAGQRK-GVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL 1592
            G +++ G   FA G W G+EL++P GKN G+V GV YF C  +YG+FAP +RV ++   ++      + + N   P        T       ++     AK  T L +S               L  G +VL+    +   +R+VGPT+F+ G W G+EL    GKNDGAV   RYF C  N GV    SR+
Sbjct:  498 GTIKFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRVQRLSDSLDTLSEISSNKQNHSYPGFRRSFSTTSASSQKEINRRNAFAKTKTTLRRSWSSSTTAGGLEGTVKLHEGSQVLLTSSNEMATVRYVGPTDFASGIWLGLELRSAKGKNDGAVGDKRYFTCKPNYGVLVRPSRV 689          

HSP 5 Score: 62.003 bits (149), Expect = 1.706e-8
Identity = 45/163 (27.61%), Postives = 70/163 (42.94%), Query Frame = 3
Query:  819 PETGVLSPPIRRQTPGPFPPSHLDLVSISDENEIYEAP---TPVSRPVSMWRDEIDNRNLASPSRVSAKVT---------LQAMXXXXXXXXXXXXXXXXSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEK 1271
            P  G+ +PP R Q       S   L  IS   + +  P      S   +  + EI+ RN  + ++ + + +         L+    L     ++    +    +RY GP +FA G+W G+EL   +GKN G V    YF C+  YGV    +RV+  GI   K
Sbjct:  539 PRYGIFAPPSRVQR---LSDSLDTLSEISSNKQNHSYPGFRRSFSTTSASSQKEINRRNAFAKTKTTLRRSWSSSTTAGGLEGTVKLHEGSQVLLTSSNEMATVRYVGPTDFASGIWLGLELRSAKGKNDGAVGDKRYFTCKPNYGVLVRPSRVTYRGISGSK 698          

HSP 6 Score: 55.0694 bits (131), Expect = 2.153e-6
Identity = 23/61 (37.70%), Postives = 35/61 (57.38%), Query Frame = 3
Query: 1845 VLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRIS 2027
            V+   ++G +R+ G   F+ G W G+EL    GK++G V   +YF C P +GI    S+IS
Sbjct:  290 VIAGQKVGTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGRVQYFKCAPKYGIFAPLSKIS 350          
BLAST of CAP-Gly domain-containing linker protein vs. UniProt/SwissProt
Match: sp|Q5U243|CLIP3_XENLA (CAP-Gly domain-containing linker protein 3 OS=Xenopus laevis OX=8355 GN=clip3 PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.813e-44
Identity = 89/181 (49.17%), Postives = 116/181 (64.09%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDT--------GLNKSPL--QIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLH 1595
            +G LR+CG  EFA G W GVELDEP GKN G+V G+ YF C  + G+FAPV+++SK     ++ P  VT      P+T  VD   V   G   K  T         L++  L  +IGD+VLVAGQ++G++RF G T+F+PG W+GIEL+KP GK+DG+V GVRYF C A  GVFAP SR+ 
Sbjct:  297 AGTLRFCGTTEFASGQWVGVELDEPDGKNDGSVGGIRYFICPPKQGIFAPVSKISKAP---DQPPSSVTS----TPRTPRVDFSRVTGKGRKEKKATHKKSLSVGSLDREGLKIEIGDQVLVAGQKQGIVRFYGKTDFAPGYWFGIELEKPTGKHDGSVFGVRYFTCSAKNGVFAPPSRVQ 470          

HSP 2 Score: 156.377 bits (394), Expect = 3.041e-39
Identity = 80/207 (38.65%), Postives = 121/207 (58.45%), Query Frame = 3
Query:   96 ERRKYPETHPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLL--DRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLP 704
            E+R+ P  HP+  APL     +Y   FFD +   C ++LL  + ++ +LFA++RQW   VQ +++ I  ++ K G +VNDRDGLTDMT+LHYA K+GA   G+   AVR+   LL  GA +  R +WT+M  LHYA YFDV   +  LL   +   + + C +   G  +HIAA+ L L   + L++ GA+    + + + P D +P
Sbjct:   26 EKRRRPVVHPSAPAPLP---KDYAFTFFDPNDPACQEILLDPQTSVPELFAIIRQWVPQVQHKIDIIGSEILKRGCHVNDRDGLTDMTLLHYACKAGAHGVGDPAAAVRLTNQLLLLGADITLRSRWTNMNALHYAAYFDVPELLRTLLKASKPKVLNSTCSDFNHGTAMHIAASNLCLGAVKCLLEHGANPSARNSKSQVPADVVP 229          

HSP 3 Score: 131.339 bits (329), Expect = 9.011e-31
Identity = 75/222 (33.78%), Postives = 111/222 (50.00%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNEL-------GIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKG 2036
            L++GDR+L+  ++ G +RF G TEF+ G W G+ELD+P GKNDG+V G+RYF C   +G+FAP+S+            I KA  Q      SS+ +  +T ++                  S       +    T+    S     REGL++   +++       GI+R+ G  +F+ G W G+EL   TGKHDG V   RYF C   +G+   PSR+   G
Sbjct:  284 LKLGDRILLDAEKAGTLRFCGTTEFASGQWVGVELDEPDGKNDGSVGGIRYFICPPKQGIFAPVSK------------ISKAPDQ----PPSSVTSTPRTPRVD----------------FSRVTGKGRKEKKATHKKSLSVGSLDREGLKIEIGDQVLVAGQKQGIVRFYGKTDFAPGYWFGIELEKPTGKHDGSVFGVRYFTCSAKNGVFAPPSRVQRMG 473          

HSP 4 Score: 69.707 bits (169), Expect = 5.184e-11
Identity = 29/57 (50.88%), Postives = 41/57 (71.93%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVG 1256
            GI+R+ G  +FA G W G+EL++P GK+ G+V GV YF C  + GVFAP +RV ++G
Sbjct:  417 GIVRFYGKTDFAPGYWFGIELEKPTGKHDGSVFGVRYFTCSAKNGVFAPPSRVQRMG 473          
BLAST of CAP-Gly domain-containing linker protein vs. UniProt/SwissProt
Match: sp|Q5R686|CLIP3_PONAB (CAP-Gly domain-containing linker protein 3 OS=Pongo abelii OX=9601 GN=CLIP3 PE=2 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 5.497e-43
Identity = 85/180 (47.22%), Postives = 114/180 (63.33%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVT--PRNPLNPQTVVVDHGTVDISGVAAKVDT-------GLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLH 1595
            +G LR+CG  EFA G W GVELDEP+GKN G+V GV YF C  + G+FA V+++SK    V+  P  VT  PR P    + V   G  +  G      +         +++  ++GD+VLVAGQ++G++RF G T+F+PG WYGIELD+P GK+DG+V GVRYF C    GVFAP SR+ 
Sbjct:  307 TGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKQGLFASVSKISKA---VDAPPSSVTSTPRTPRMDFSRVTGKGRREHKGKKKTPSSPSLGSLQQRDRAKAEVGDQVLVAGQKQGIVRFYGKTDFAPGYWYGIELDQPTGKHDGSVFGVRYFTCPPRHGVFAPASRIQ 483          

HSP 2 Score: 161.77 bits (408), Expect = 5.997e-41
Identity = 86/208 (41.35%), Postives = 125/208 (60.10%), Query Frame = 3
Query:   96 ERRKYPETHPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLLDRGADIRAI---CRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLP 704
            ERR+ P  HP+  APL     +Y   FFD +   C ++L   + TI +LFA++RQW   VQ +++ I  ++ + G +VNDRDGLTDMT+LHYA K+GA   G+   AVR+   LL  GA +  R +WT+M  LHYA YFDV   V +LL +GA  R +   C +   G+ LHIAA+ L L  A+ L++ GA+    + + + P + +P
Sbjct:   36 ERRQKPVVHPSAPAPLPK---DYAFTFFDPNDPACQEILFDPQTTIPELFAIVRQWVPQVQHKIDVIGNEILRRGCHVNDRDGLTDMTLLHYACKAGAHGVGDPAAAVRLSQQLLALGADVTLRSRWTNMNALHYAAYFDVPDLVRVLL-KGARPRVVNSTCSDFNHGSALHIAASSLCLGAAKCLLEHGANPALRNRKGQVPAEVVP 239          

HSP 3 Score: 140.198 bits (352), Expect = 9.745e-34
Identity = 79/216 (36.57%), Postives = 113/216 (52.31%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL-CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKG 2036
            L++GDRVL+ GQ+ G +RF G TEF+ G W G+ELD+P GKNDG+V GVRYF C   +G+FA +S+  I ++       + +T +    + S +    + +      +P           +S S+ +  Q +          R     G QVL    + GI+R+ G  +F+ G W G+EL   TGKHDG V   RYF CPP HG+    SRI   G
Sbjct:  294 LRLGDRVLLDGQKTGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKQGLFASVSK--ISKAVDAPPSSVTSTPRTPRMDFSRVTGKGRREHKGKKKTP-----------SSPSLGSLQQRD----------RAKAEVGDQVLVAGQKQGIVRFYGKTDFAPGYWYGIELDQPTGKHDGSVFGVRYFTCPPRHGVFAPASRIQRIG 486          
BLAST of CAP-Gly domain-containing linker protein vs. UniProt/SwissProt
Match: sp|B9EHT4|CLIP3_MOUSE (CAP-Gly domain-containing linker protein 3 OS=Mus musculus OX=10090 GN=Clip3 PE=1 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 7.617e-43
Identity = 88/180 (48.89%), Postives = 112/180 (62.22%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVT--PRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQ-------IGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLH 1595
            +G LR+CG  EFA G W GVELDEP+GKN G+V GV YF C  + G+FA V++VSK    V+  P  VT  PR P    + V   G  +  G      +    S  Q       +GD+VLVAGQ++G++RF G T+F+PG WYGIELD+P GK+DG+V GVRYF C    GVFAP SR+ 
Sbjct:  307 TGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKQGLFASVSKVSKA---VDAPPSSVTSTPRTPRMDFSRVTGKGRREHKGKKKSPSSPSLGSLQQREGAKAEVGDQVLVAGQKQGIVRFYGKTDFAPGYWYGIELDQPTGKHDGSVFGVRYFTCAPRHGVFAPASRIQ 483          

HSP 2 Score: 161.77 bits (408), Expect = 6.112e-41
Identity = 86/208 (41.35%), Postives = 125/208 (60.10%), Query Frame = 3
Query:   96 ERRKYPETHPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLLDRGADIRAI---CRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLP 704
            ERR+ P  HP+  APL     +Y   FFD +   C ++L   + TI +LFA++RQW   VQ +++ I  ++ + G +VNDRDGLTDMT+LHYA K+GA   G+   AVR+   LL  GA +  R +WT+M  LHYA YFDV   V +LL +GA  R +   C +   G+ LHIAA+ L L  A+ L++ GA+    + + + P + +P
Sbjct:   36 ERRQKPVVHPSAPAPLPK---DYAFTFFDPNDPACQEILFDPKTTIPELFAIVRQWVPQVQHKIDVIGNEILRRGCHVNDRDGLTDMTLLHYACKAGAHGVGDPAAAVRLSQQLLALGADVTLRSRWTNMNALHYAAYFDVPDLVRVLL-KGARPRVVNSTCSDFNHGSALHIAASNLCLGAAKCLLEHGANPALRNRKGQVPAEVVP 239          

HSP 3 Score: 129.798 bits (325), Expect = 2.748e-30
Identity = 78/226 (34.51%), Postives = 111/226 (49.12%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTT----HQFNSPTYFGDSSGRVFLREGLQ-------VLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKG 2036
            L++GDRVL+ GQ+ G +RF G TEF+ G W G+ELD+P GKNDG+V GVRYF C   +G+FA +S++                                  +  +A   +  S  ++P M  S V+      H+    +    S G +  REG +       ++   + GI+R+ G  +F+ G W G+EL   TGKHDG V   RYF C P HG+    SRI   G
Sbjct:  294 LRLGDRVLLDGQKTGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKQGLFASVSKV---------------------------------SKAVDAPPSSVTSTPRTPRMDFSRVTGKGRREHKGKKKSPSSPSLGSLQQREGAKAEVGDQVLVAGQKQGIVRFYGKTDFAPGYWYGIELDQPTGKHDGSVFGVRYFTCAPRHGVFAPASRIQRIG 486          

HSP 4 Score: 73.1738 bits (178), Expect = 3.759e-12
Identity = 29/57 (50.88%), Postives = 42/57 (73.68%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVG 1256
            GI+R+ G  +FA G W G+ELD+P GK+ G+V GV YF C  ++GVFAP +R+ ++G
Sbjct:  430 GIVRFYGKTDFAPGYWYGIELDQPTGKHDGSVFGVRYFTCAPRHGVFAPASRIQRIG 486          
BLAST of CAP-Gly domain-containing linker protein vs. TrEMBL
Match: A0A0B7AJW1 (Uncharacterized protein OS=Arion vulgaris OX=1028688 GN=ORF119659 PE=4 SV=1)

HSP 1 Score: 478.404 bits (1230), Expect = 1.626e-156
Identity = 276/666 (41.44%), Postives = 379/666 (56.91%), Query Frame = 3
Query:  120 HPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQG--EEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLLD--RGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVS--ELPSNMTTIRNNLGTLLCPTSPPGHTYYNANHRKPPETGVLSPPIRRQTPGPFPPSHLDLVSISDENEIYEAPTPVSRPVSMWRDEIDNRNLASPSRVSAKVTLQAMXXXXXXXXXXXXXXXXSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSP------LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQ---TDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLIPPELA 2072
            HP V+ PLCD+C    L FFD  C  C ++L++   T+ ++FA++RQWT   Q  L  +++++ K GAN+NDRDGLTDMT+LHYA+KSGA G  + E A+RV+  L+D GA +  RC+WT+MT LHYA YFDV P +++LL   +  DI + C E   G  LHIAA+ LA +  ++L++ GA+    DD  R P DC+P  S  E  SNM  +   +   L    PP                              PP + DLV                                      +KVTLQA+GL LGD++LV G    SGILRYCGP +FA G+WAGVELDE  GKN+G+V G+ YF C    G+FAPV++++K+G  +++       + P  P+    D  ++D+S V AKVDTGL K        +++GDRV+VAGQRKG +RF+G  +F+ G WYG+ELD+PVGKNDG+VNGVRYF C A  GVFAPL+R+  L  R             R S+  S+ T S    +++L   S+    +  Q+P       S  HQ  S +     S    L  G  VLCNNELG+IRY+G     +G W+GVELR   GK+DG V  KRYF CPP+ G++V+PSR++++GINGA ++  + A
Sbjct:   30 HPCVDPPLCDSCTRLELSFFDPICPDCREILVNPSTTVPEIFAIMRQWTPQTQQSLALLVDEILKRGANINDRDGLTDMTLLHYASKSGAAGIGDPESAMRVVLMLIDAGADVNIRCRWTNMTALHYAAYFDVVPVLKILLKATKALDIDSTCSEFEHGTALHIAASNLAHEACKILLQNGANPSLKDDLGRMPVDCVPDPSSYEEDSNMQQLVAKVKKTLSEALPPSQKQ--------------------------PPPNYDLVQ-------------------------------------SKVTLQALGLALGDKILVGGMK--SGILRYCGPTDFAPGLWAGVELDESSGKNNGSVGGINYFNCPNNCGIFAPVSKITKLG-AIQRS------QTPPTPKWFSRDAASIDLSRVTAKVDTGLYKQMSLDTEIIEVGDRVVVAGQRKGTVRFIGEAQFAVGMWYGVELDRPVGKNDGSVNGVRYFNCKAKHGVFAPLTRIQKLGDR-------------RFSSSESLDTISWGALSEKLERKSTSGMSNHIQTP-----PRSLKHQNLSLSRTPGQSPSQVLELGAMVLCNNELGVIRYMGPTELGEGTWLGVELRKPKGKNDGSVQGKRYFTCPPNCGLLVKPSRVTLRGINGAKILAEQNA 605          
BLAST of CAP-Gly domain-containing linker protein vs. TrEMBL
Match: A0A504Y6Y0 (CAP-Gly domain-containing linker protein 4 OS=Fasciola gigantica OX=46835 GN=FGIG_01115 PE=4 SV=1)

HSP 1 Score: 481.485 bits (1238), Expect = 3.470e-153
Identity = 325/891 (36.48%), Postives = 423/891 (47.47%), Query Frame = 3
Query:   90 ISERRKYPETHPAVEAPLCDNCAEY---------------------------------GLCFFDLD-----CEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQGEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLLDRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRG-----------ADRYFLDDRQRTPYDCLPHVSELPSNMTTIRNNLGTLLCPT---------------------SP-----------PGHTYYNANHRKPP------------ETGVLSPPIRRQTPGP---------FPPSHLDLVSISDENEIYEAPTPVS--RPVSMWRDEID-NRNLASPSRVSAKVTLQAM-----------------------XXXXXXXXXXXXXXXXSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSK--------------VGIEVEKRPRCVTPRN--PLNP--------------------------------------------QTVVVDHGTVDISGVAAKVDTGLN-KSP--------LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHIL-----RSRHKNDEIIKATQQNRLSNHSSMMTA--SQTDQLSNASSPNH---LSMAQSPLMTSSSVS-----------------TTHQFNSPTYFGDSSGRVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLIPPELAYLEKKI 2090
            I+ RR +P  HP  E P C+ C +                                     F  L      CE C  ++ S  +TI  LFAVLRQWT   Q+QL TI++++ + GA+V+DRDGLTDMT+LH++AKSGA G+EE A R+  +LLD GA LEARC+WTDMT LHYA YFD    VELL+ RGA + A  R       LH+AATQL+L   RVL+              A +  LD   RTPY CLP  ++L   + ++R+ L  LL P                      +P           P H+     H   P            E+G LSP + R  P P          PP+ +   S +    + + P+P +  RP +     I  N  L + S +SAKVTLQ++                                      R G LRYCGPV FA GVW GVELDEP G+N+GTV GV YF C   YGVFAP+ RV K              V   V  R R  TPR   P +P                                              +VV H  +D+S V AK+DTGL  +SP        L +GDRVLVAGQRKGVIRF+G T+F+PG WYG+ELD+ VGKN+G+V GVRYFQC    G+FAPLSRLH L     RS     +  +A        H+SM  +    T   S ++ P H    S   SP++  + V                  T  +   P  F       +L EG+QVLC  E+GI+RY+G + F++GIW+GVELR   G+HDG V  KRYF C P HG++VRPSR+  +GIN  +L+PP LA +E+++
Sbjct:  121 IAPRRSHPIVHPPCEVPFCETCRQMDADMAAAAASEANNPHHSPTPPTPASDDDDESRSWIFKQLPGPWWWCEDCQALVSSPEITIAHLFAVLRQWTPYAQLQLITIVDEILRRGAHVDDRDGLTDMTLLHFSAKSGALGDEESACRIAEFLLDNGANLEARCRWTDMTALHYAAYFDCPMLVELLIRRGAQLEARSRMADGATPLHLAATQLSLGATRVLLHASTINLVQAESDFAGKESLDGLGRTPYQCLPPTNQLAEPLCSLRDRLSELLGPAVRLPVNGLEQDPLNERTDSLPTPRLSRATTTNGFPAHSVQCNGHDSQPNPPADGMSWLVAESGYLSPRVPRVQPTPPKAPVISAEAPPAPIK--SRTPLPVVQQPPSPATLPRPSTASNKPIALNHPLPATSSISAKVTLQSLGLALDDRVCIGPGNTGNSAKMTPSRSHARDPGPIAMNSRIGHLRYCGPVAFAAGVWVGVELDEPLGRNNGTVAGVQYFSCAANYGVFAPIGRVYKAVCCDGANSQQWLPVRQSVSSRMRPGTPRQHTPQSPDHEGDASDGSRGPIRVSRSSYSLTGSLSSNENEHGSQHSGGTGSSGIVVSHPPIDVSHVTAKIDTGLRVRSPDMNASAKQLALGDRVLVAGQRKGVIRFIGQTQFAPGIWYGVELDQAVGKNNGSVAGVRYFQCAVGHGIFAPLSRLHRLPPTCVRSATSTPQCSRAVAAGL--RHASMTGSMYGPTAGSSRSTRPTHRQAWSTTTSPMVGRAVVGRPALPQELINALHAAGHTPKEIKDPPVF-------YLAEGMQVLCAGEIGILRYIGPITFAEGIWLGVELRKPRGRHDGSVAGKRYFTCQPGHGLLVRPSRVFCRGINALDLLPPPLAAMEREL 1000          
BLAST of CAP-Gly domain-containing linker protein vs. TrEMBL
Match: H2KRA0 (Phosphatidylinositol glycan class U OS=Clonorchis sinensis OX=79923 GN=CLF_105792 PE=4 SV=1)

HSP 1 Score: 477.633 bits (1228), Expect = 5.072e-151
Identity = 314/804 (39.05%), Postives = 416/804 (51.74%), Query Frame = 3
Query:   78 SVDTISERRKYPETHPAVEAPLCDNC----------------------------AEYGLCFFDLD-----CEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQGEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLLDRGADIRAICRELCRGNCLHIAATQLALDTARVLIK-----------RGADRYFLDDRQRTPYDCLPHVSELPSNMTTIRNNLGTLL-CPTS--PPG-----HTYYNAN---------------HRKPPETGVLSPP-------IRRQTPGPFPPSHLDLVS-----------ISDENEIYEAPTPVSRPVSMWRDEIDNRNLASPSRVSAKVTLQAMXXXXXXXXXXXXXXXXS--------------GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKV----GIEVEKRP---------RCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGL---------NKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKAT--QQNRLSNHSSMMTASQTDQLSNASSPNH----------LSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLIPPELAYLEKKI 2090
            S   +  RR YP +HP  E P C +C                            A     F  L      CE C   + S ++TI  LFAVLRQWT   Q+QL T++E++F+ GA+V+DRDGLTDMT+LH+AAKSGA G+EE A R+  +LL+ GA LEARC+WTDMTPLHYA YFD    VEL + RGA + A          LH+ A QL+L  ARVL+            R A +  LD   RTPYDCLP V++L   + ++R+ L  LL  PT   P G     H  YN                   KPP   V + P        R+ TP P   S L   S            S+ + I   PT  +   +  +  + N  L S S VSAKVTLQA+GL L DRV +      S              G LRYCGPV FA GVW GVELD+P G+N+GTV+GV YF C   YG+FAP+ RV K     G   + RP         +  + R+    Q V    G       A+  DT L         +    Q+GDRVLVAGQR+GVIR++G TEF+PGTWYGIELD+P+GKN+G+V GVRYFQC    G+FA ++R+  L +R    +I K    + N   + S       +D+L   +S +           +     P    ++++           G      ++ EG+QVLC+ E+GI+RY+G + F+DGIW+G+ELR   G+HDG V  KRYF C P HG++VRPSR+  +GIN  NL+PP LA +E+++
Sbjct:  242 STSELPRRRSYPISHPPSEVPFCASCRQQDEEMLAASEEKSPSPTGVPPLEDEDASRSWIFKQLPGPWWWCEDCQASVSSPQITIPHLFAVLRQWTPYAQLQLITVVEEIFRRGAHVDDRDGLTDMTLLHFAAKSGAVGDEESACRIAEFLLNGGANLEARCRWTDMTPLHYAAYFDCPMLVELFIQRGAQLEARSSLADEATPLHLCAIQLSLGAARVLLHASVQSLAQAGVRYAGKDALDALGRTPYDCLPPVTQLAEPLCSLRDRLAELLGSPTKDRPAGFPIGTHADYNVTTPDGGIMPVENGTYVSTKPPYQPVATAPSLENAASSRQPTPQPDGLSWLVAESGYLSPRNRRSTASNSDSISPPPTRPATAPASRQPHVINHPLPSTSSVSAKVTLQALGLSLDDRVCIGPGSSASLNGSVDRVSVNGRIGRLRYCGPVGFASGVWVGVELDQPLGRNNGTVSGVQYFSCAPNYGIFAPIGRVYKAVSDGGATQKWRPIQGKLPSSAKSPSMRSSAQKQQV----GATSDEDAASSTDTSLRIQSSTDGSDTQQFQLGDRVLVAGQRRGVIRYIGSTEFAPGTWYGIELDQPLGKNNGSVAGVRYFQCPVGHGIFASVNRIQRLPNRPGTPQISKPIPFKPNTSMSGSWYGDPVGSDRLRTRTSWSTTTSPMIGRAVVGRPSLPAELVNALTAAGHGAKQAMAGKDEPAFYITEGMQVLCSGEMGIVRYIGPITFADGIWLGIELRKPRGRHDGSVAGKRYFNCRPGHGLLVRPSRVFCRGINAVNLLPPALAAIEREL 1041          
BLAST of CAP-Gly domain-containing linker protein vs. TrEMBL
Match: G4LY96 (Restin-like OS=Schistosoma mansoni OX=6183 GN=Smp_133390 PE=4 SV=1)

HSP 1 Score: 466.848 bits (1200), Expect = 1.181e-144
Identity = 308/837 (36.80%), Postives = 420/837 (50.18%), Query Frame = 3
Query:   99 RRKYPETHPAVEAPLCDNC--------AEYG-------------------LCFFDLD---------------CEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQGEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLLDRGADIRAICRELCRGNCLHIAATQLALDTARVLIK-------RGADRYFLDDRQ----RTPYDCLPHVSELPSNMTTIRNNLGTLLCP------------------TSPPGHTYYNANHRKPPETGVLSPPIRRQTPGPFPPSHLD-LVSISD--ENEIYEAPTPVSRPVSMWRDEIDNRN-LASPSR---VSAKVTLQAM------------------XXXXXXXXXXXXXXXXSGI------LRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSK-VGIEVEKRPRCVTP-------------------------------------------RNPLNPQTVVVDHGTVDISGVAAKVDTGLN-KSP-------LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTAS----QTDQLSNAS-----SPNHLSMAQSPLMTSSSVST----THQFNSPTYFGDSSGRVF------LREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLIPPELAYLEKKI 2090
            RR  P  HPA E P C+NC         EY                    +   D +               CE C + + S  +TI QLFA+LRQWT   Q+QL TI+E++F+ GA+V+DRDGL+DMT+LH+ AKSGA G+E+ + R+  YLLDEGA LEARCKWTDMTPLHYA YFD     +LL++RGA I A    +     LH+AA+QL+L TAR+LI+       +   RY   D +    RTPY+CLP   +LP  + T+R+ L  LL P                  TS    T Y   H         S P           + +D LV+ S         +PT + +P++      D ++   S S+   VSAKVTLQ+M                                  +G+      LRYCG V F  G+W GVELDEP G+N+G+V G+ YF C  Q+G+FAP+ RV K V I+ ++    VT                                            R P  P T+         S V AK+DTGL  +SP        QIGDRVLVAGQR+GV+RF+G T+F+PG WYGIEL++ VGKN+G++NG+RYF C    G+FAP+SR+  L +R     + K      LS++S+ MT S    Q D ++  S     +P   S   SP++  + +      T   N+    G      F      L EG+QVLC  E+GI+RY+G + F++GIW+G+ELR   G+HDG V  +RYF C P HG++VRP+R+   GIN  NL+PP LA +E+++
Sbjct:  465 RRSQPVVHPASEIPFCENCRVMDEEMSKEYDPLTGSPVNNDNDNHHLPEEMIHDDEEESRSWVMRMPVGPWWCEDCQESVTSTNLTISQLFAILRQWTPYAQLQLITIVEEIFRRGAHVDDRDGLSDMTLLHFTAKSGALGDEQASCRIANYLLDEGANLEARCKWTDMTPLHYAAYFDCPLLADLLINRGACISARTCLIDHSTPLHLAASQLSLGTARILIQAADFSVTKTGQRYDAKDAKDGYGRTPYECLPPSGQLPEPLCTLRDLLAELLRPTSPQQTDDIDIEKKIIQTTSNDYSTEYRNEHITNDYVNNQSLPTENSHSQQSTYNGVDWLVAESGFLSPRTKRSPTNIQQPLNNNNINNDVKSPTTSQSKKFTVSAKVTLQSMGLALGDRVCIAPGNSVPSSSSAMQTSGKNQPQLTTGVSGRIGKLRYCGSVSFGSGIWVGVELDEPVGRNNGSVAGIQYFSCANQHGIFAPIGRVYKTVNIDGKQNWHPVTSSTNTTISNRRSSNSSNISTTSKKLNSSLRNQHKHSTTVSSQLSRTPTPPSTL---QSPSSFSHVTAKIDTGLRIRSPDISRNHHFQIGDRVLVAGQRRGVLRFIGQTQFAPGIWYGIELEQAVGKNNGSINGIRYFDCAVGHGIFAPISRIQKLPARPNTPNLTK------LSDNSTTMTTSFHSEQVDGVAGRSWCCRRTP--WSSTTSPMIGRAVIGRPPLPTELVNALKAVGRVPAESFEEPVFYLTEGMQVLCAGEIGIVRYIGPITFAEGIWLGIELRKPRGRHDGCVAGRRYFTCRPGHGLLVRPARVFCHGINAVNLLPPALAEIERQL 1290          
BLAST of CAP-Gly domain-containing linker protein vs. TrEMBL
Match: T1KTP7 (Uncharacterized protein OS=Tetranychus urticae OX=32264 GN=107367353 PE=4 SV=1)

HSP 1 Score: 429.483 bits (1103), Expect = 2.452e-137
Identity = 266/688 (38.66%), Postives = 381/688 (55.38%), Query Frame = 3
Query:   72 VMSVDTISERRKYPETHPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQGEEEL--AVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLL---DRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNMTTIRNNLGTLLCPTSPPGHTYYNANHRKPPETGVLSPPIRRQTPGPFPPSHLDLVSISDENEIYEAPTPVSRPVSMWRDEIDNRNLASPSRVSAKVTLQAMXXXXXXXXXXXXXXXXSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTV-VVDHGTVDISGVAAKVDTGLNK------SPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSR-LHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLM----------TSSSVSTTHQFNS--PTYFGDSSGRVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLIP 2060
            V  + TI  +   P+ H A + P C  C +    FF++ C  C ++L +   TI QLFA+LRQW   +Q  +E ++ ++ K GA+ ND+DGLTDMT+LHYA K+G+ G  ++  A+R +  L+ +GA L  RC+WTDM  +HYAVYFDV+  V+ LL   DR A I  +C +   G  LHIAA  L +D+ +VL+K GA+    DD  RT  DC+P       + TT+ N+     C  S  G        R   E   LS                   +I  E   +E P                        V+ KV LQA+GL LGD+V + G    SGILR+CG  +FA G+WAG+EL+EP+GK+ GT  GV YF+C   +G+FAP++++ +     +   R +   N    QTV +V+H  VD+S V  KV+TGLN       +  ++GD+V++  +R GV+RFVG T F+ G WYGIEL KP+GKNDG++ GV+YF C  + G+F P  R L + +S  K  + I A  ++     S    +        A SP  L+M +              TS  V++++  NS  PT    SS   +L  G+ V  NNE+G++RY+G V+F+DGIW+G+ELR   GK+DG V  KRYF C P HG++VRP ++SV+GING+ L+P
Sbjct:   43 VYEIMTIESKWNSPKIHVASDPPFCKTCEQLDTTFFNIQCHNCREILTNPDTTISQLFAILRQWVPQIQYCIELLVAEILKRGAHHNDKDGLTDMTLLHYACKAGSSGVGDVRAALRTVHLLISKGADLSIRCRWTDMAAIHYAVYFDVSEIVDYLLTISDR-ACIDDVCHDFEGGTPLHIAAANLCIDSVKVLLKHGANVLLKDDHGRTALDCVP-------DPTTLLNS-----CILSKQGAVEVATELRALLEKAFLS-------------------TIPGETFTFEGP------------------------VTGKVVLQALGLQLGDKVAINGVK--SGILRFCGATQFACGIWAGIELEEPEGKHDGTFQGVTYFRCPPNHGIFAPISKICRY----DNLWRNIRGTN----QTVRIVNHPGVDVSRVTPKVETGLNSLKYRSANVAEVGDKVVLVDKRIGVVRFVGETSFASGIWYGIELSKPIGKNDGSIEGVKYFDCKPSHGLFVPPGRILRVTKSPQKPIDEIFADDES-----SECSLSLSRSSSERAKSP--LTMRRLRSRSSLKSSSFSPTSYPVTSSNNLNSLPPTNNNSSS---WLTLGVNVFVNNEVGVVRYIGPVDFADGIWLGLELRSANGKNDGAVQGKRYFTCKPGHGLLVRPKKVSVRGINGSKLLP 654          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Cavefish
Match: clip3 (CAP-Gly domain containing linker protein 3 [Source:NCBI gene;Acc:103042224])

HSP 1 Score: 162.155 bits (409), Expect = 3.615e-42
Identity = 82/209 (39.23%), Postives = 125/209 (59.81%), Query Frame = 3
Query:   90 ISERRKYPETHPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLLDRGAD--IRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLP 704
            + E RK P  HP+ +APL     +Y   FFD +   C+++L   R TI +LFA++RQW   VQ +++ I E++ K G +VNDRDGLTDMT+LHY+ K+GA   G+ E ++R+   L+  GA +  R +WT+M  LHYA YFDV   + +LL       + + C +   G  LHIAA+ L L   + L++ GA+    +D+ + P + +P
Sbjct:   22 VHEPRKKPMVHPSAQAPLP---KDYTFTFFDPNDPACLEILTDPRTTIPELFAIIRQWVPQVQHKIDIIGEEILKRGCHVNDRDGLTDMTLLHYSCKAGAHGVGDPEASLRLTNQLIALGADVSLRSRWTNMNALHYAAYFDVPELIRVLLKASKPRVLNSTCSDFYHGTALHIAASNLCLGAVKCLLEHGANPTVRNDKGQGPAEVVP 227          

HSP 2 Score: 156.762 bits (395), Expect = 2.810e-40
Identity = 80/185 (43.24%), Postives = 115/185 (62.16%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVT--PRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPL------------QIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLH 1595
            +G LR+CG  EFA G W G+ELDEP+GKN G+V GV YF C  + G+FAPV++++K    V++ P  VT  P+ P    + V   G +       + +  + K  L            ++GD+VLVAGQ++G++RF G T+F+PG W+G+EL++  GK+DG+V GVRYF C    GVFAP SR+ 
Sbjct:  295 AGTLRFCGTTEFASGQWVGIELDEPEGKNDGSVGGVRYFICPPKLGIFAPVSKINKA---VDQTPSSVTSTPKTPRMDFSRVT--GKIKKDKKEKEREKNMRKKSLSVASLDPDGVKVEVGDQVLVAGQKQGIVRFYGKTDFAPGYWFGVELEQATGKHDGSVFGVRYFTCLPKYGVFAPPSRVQ 474          

HSP 3 Score: 132.88 bits (333), Expect = 2.593e-32
Identity = 79/216 (36.57%), Postives = 112/216 (51.85%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL-CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKG 2036
            L++GDRV +   + G +RF G TEF+ G W GIELD+P GKNDG+V GVRYF C    G+FAP+S+++  ++  +    + +T +    + S +    + D+       N         M   S+S            D  G V +  G QVL    + GI+R+ G  +F+ G W GVEL   TGKHDG V   RYF C P +G+   PSR+   G
Sbjct:  282 LKLGDRVCLDDMKAGTLRFCGTTEFASGQWVGIELDEPEGKNDGSVGGVRYFICPPKLGIFAPVSKIN--KAVDQTPSSVTSTPKTPRMDFSRVTGKIKKDKKEKEREKN---------MRKKSLSVASL--------DPDG-VKVEVGDQVLVAGQKQGIVRFYGKTDFAPGYWFGVELEQATGKHDGSVFGVRYFTCLPKYGVFAPPSRVQRIG 477          

HSP 4 Score: 69.707 bits (169), Expect = 6.034e-12
Identity = 30/57 (52.63%), Postives = 41/57 (71.93%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVG 1256
            GI+R+ G  +FA G W GVEL++  GK+ G+V GV YF C  +YGVFAP +RV ++G
Sbjct:  421 GIVRFYGKTDFAPGYWFGVELEQATGKHDGSVFGVRYFTCLPKYGVFAPPSRVQRIG 477          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Cavefish
Match: si:dkeyp-47f9.4 (si:dkeyp-47f9.4 [Source:ZFIN;Acc:ZDB-GENE-131126-9])

HSP 1 Score: 147.132 bits (370), Expect = 2.418e-37
Identity = 82/228 (35.96%), Postives = 120/228 (52.63%), Query Frame = 3
Query:   63 DVLVMSVDTISERRKYPETHPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLL--DRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNMT 734
            D ++     +S     P  H    AP+     +    FFD     C +VLL  + ++ +LFAVLRQW   VQ  +  I  ++ K G  VNDRDGLTDM++LHY  K+GA   G+ E A      LL  GA    R +WT+M+ LHYA YFDV P + ++L   +  ++ A C +   G  LHIAA+ +     R L++ GA+  F +D+ + P D +P   ++P  M 
Sbjct:   38 DAVLERYSVVSHTAARPVIHQVASAPMP---TDSDFSFFDPTDPQCREVLLDPQTSVPELFAVLRQWVPQVQKNISRIGNEILKRGCGVNDRDGLTDMSLLHYCCKAGAPGIGDAENAASFARKLLSLGAEPSLRSRWTNMSALHYAAYFDVPPLIRVVLQASQPGEVDATCSDFDFGTALHIAASNICTAAVRCLLELGANPAFRNDKGQCPADVVPEPLDMPLEMA 262          

HSP 2 Score: 92.4337 bits (228), Expect = 2.864e-19
Identity = 40/91 (43.96%), Postives = 60/91 (65.93%), Query Frame = 3
Query: 1344 ISGVAAKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHK 1616
            +S +  K  + L+   + +GD+V++AGQ+ G +RF G TEF+ G W G+EL +P GKNDG+V GV+YF C    G+FAPLS++     R +
Sbjct:  294 LSLLTEKARSQLSSMGICLGDKVIIAGQKVGTLRFCGSTEFAGGLWAGVELQQPEGKNDGSVAGVQYFTCPVKHGIFAPLSKITKFSERRR 384          

HSP 3 Score: 92.0485 bits (227), Expect = 4.312e-19
Identity = 45/97 (46.39%), Postives = 64/97 (65.98%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTG 1376
            G LR+CG  EFA G+WAGVEL +P+GKN G+V GV YF C  ++G+FAP+++++K      +RP   +PR P  P   V     +D++ V +KVDTG
Sbjct:  324 GTLRFCGSTEFAGGLWAGVELQQPEGKNDGSVAGVQYFTCPVKHGIFAPLSKITK--FSERRRP---SPRQPAAP---VRPPRHIDLARVKSKVDTG 412          

HSP 4 Score: 62.003 bits (149), Expect = 1.298e-9
Identity = 24/61 (39.34%), Postives = 38/61 (62.30%), Query Frame = 3
Query: 1845 VLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRIS 2027
            ++   ++G +R+ G+  F+ G+W GVEL+   GK+DG V   +YF CP  HGI    S+I+
Sbjct:  317 IIAGQKVGTLRFCGSTEFAGGLWAGVELQQPEGKNDGSVAGVQYFTCPVKHGIFAPLSKIT 377          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Cavefish
Match: ENSAMXT00000039156.1 (pep primary_assembly:Astyanax_mexicanus-2.0:20:1742506:1745198:-1 gene:ENSAMXG00000038021.1 transcript:ENSAMXT00000039156.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 138.272 bits (347), Expect = 5.366e-36
Identity = 74/178 (41.57%), Postives = 108/178 (60.67%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGI-EVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLN--------KSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL 1592
            G +++ G  +FA G WAG+ LDEP GKN G+V GV YFQCE+  G+F   +++S+  I E E  P       P + QT   +  + +++   +   T L+        K  L++GDRVL+ G + GV+RF+G T+F+ G W G+ELD+P+GKNDGAV G  YFQC    G+FAP  ++
Sbjct:   63 GYIQFIGATQFAPGQWAGIVLDEPIGKNDGSVAGVRYFQCEDLRGIFTRPSKLSRAPIPERETNP-------PADGQTAAANKTSTNLAKDTSGSVTNLSEADSAKKAKRELKLGDRVLIGGTKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGA-YFQCLPKYGLFAPTHKV 232          

HSP 2 Score: 117.087 bits (292), Expect = 8.997e-29
Identity = 72/207 (34.78%), Postives = 109/207 (52.66%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNN-ELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMV 2009
              +G+RV V G + G I+F+G T+F+PG W GI LD+P+GKNDG+V GVRYFQC   RG+F   S+L             +A    R +N  +       D  + A++    ++A+    TS SV+   + +S         +  L+ G +VL    + G++R++G  +F+ G W GVEL    GK+DG V    YF C P +G+  
Sbjct:   49 FSVGERVWVNGNKPGYIQFIGATQFAPGQWAGIVLDEPIGKNDGSVAGVRYFQCEDLRGIFTRPSKLS------------RAPIPERETNPPA-------DGQTAAANKTSTNLAKD---TSGSVTNLSEADS-----AKKAKRELKLGDRVLIGGTKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGA-YFQCLPKYGLFA 227          

HSP 3 Score: 72.7886 bits (177), Expect = 1.218e-13
Identity = 31/59 (52.54%), Postives = 42/59 (71.19%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGI 1259
            +G++R+ G  +FA G W GVELDEP GKN G V G  YFQC  +YG+FAP  +V+++G 
Sbjct:  180 AGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGA-YFQCLPKYGLFAPTHKVTRIGF 237          

HSP 4 Score: 58.151 bits (139), Expect = 8.770e-9
Identity = 28/65 (43.08%), Postives = 38/65 (58.46%), Query Frame = 3
Query: 1836 GLQVLCN-NELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRIS 2027
            G +V  N N+ G I+++GA  F+ G W G+ L    GK+DG V   RYF C    GI  RPS++S
Sbjct:   52 GERVWVNGNKPGYIQFIGATQFAPGQWAGIVLDEPIGKNDGSVAGVRYFQCEDLRGIFTRPSKLS 116          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Cavefish
Match: clip1a (CAP-Gly domain-containing linker protein 1-like [Source:NCBI gene;Acc:111191575])

HSP 1 Score: 137.502 bits (345), Expect = 8.935e-33
Identity = 71/193 (36.79%), Postives = 104/193 (53.89%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSK------------------------VGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL 1592
            G +++ G  +FA G WAG+ LDE  GKN G+V GV YFQ E   G+F   +++S+                        V        +  TP  P  P + +    +  +S ++        +  L++GDRVLVAG + GV+RF+G T+F+ G W G+ELD+P+GKNDGAV G RYFQC    G+FAP+ ++
Sbjct:   69 GFIQFLGETQFAPGQWAGIVLDEAIGKNDGSVAGVRYFQSEPLRGIFTRPSKLSRTEGEANGTTTAPPSPTPSTTAAPSVSSTTSTTKKAATPTKPDAPASNLTRANSESMSNLSETGSVKKGERELKMGDRVLVAGSKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQPKYGLFAPVHKV 261          

HSP 2 Score: 123.25 bits (308), Expect = 2.956e-28
Identity = 75/216 (34.72%), Postives = 116/216 (53.70%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL-CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKG 2036
             +IGDRV V G + G I+F+G T+F+PG W GI LD+ +GKNDG+V GVRYFQ    RG+F   S+L   R+  + +    A      S  ++   +S T     A++P       S L  ++S S ++   + +      G   L+ G +VL   ++ G++R++G  +F+ G W GVEL    GK+DG V   RYF C P +G+     +++  G
Sbjct:   55 FEIGDRVWVNGNKPGFIQFLGETQFAPGQWAGIVLDEAIGKNDGSVAGVRYFQSEPLRGIFTRPSKLS--RTEGEANGTTTAPPSPTPSTTAAPSVSSTTSTTKKAATPTKPDAPASNLTRANSESMSNLSETGSV---KKGERELKMGDRVLVAGSKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQPKYGLFAPVHKVTRIG 265          

HSP 3 Score: 82.0333 bits (201), Expect = 1.865e-15
Identity = 32/58 (55.17%), Postives = 44/58 (75.86%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVG 1256
            +G++R+ G  +FA G W GVELDEP GKN G V G  YFQC+ +YG+FAPV +V+++G
Sbjct:  208 AGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQPKYGLFAPVHKVTRIG 265          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Cavefish
Match: clip1a (CAP-Gly domain-containing linker protein 1-like [Source:NCBI gene;Acc:111191575])

HSP 1 Score: 137.502 bits (345), Expect = 9.048e-33
Identity = 71/193 (36.79%), Postives = 104/193 (53.89%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSK------------------------VGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL 1592
            G +++ G  +FA G WAG+ LDE  GKN G+V GV YFQ E   G+F   +++S+                        V        +  TP  P  P + +    +  +S ++        +  L++GDRVLVAG + GV+RF+G T+F+ G W G+ELD+P+GKNDGAV G RYFQC    G+FAP+ ++
Sbjct:   69 GFIQFLGETQFAPGQWAGIVLDEAIGKNDGSVAGVRYFQSEPLRGIFTRPSKLSRTEGEANGTTTAPPSPTPSTTAAPSVSSTTSTTKKAATPTKPDAPASNLTRANSESMSNLSETGSVKKGERELKMGDRVLVAGSKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQPKYGLFAPVHKV 261          

HSP 2 Score: 123.25 bits (308), Expect = 2.995e-28
Identity = 75/216 (34.72%), Postives = 116/216 (53.70%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVL-CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKG 2036
             +IGDRV V G + G I+F+G T+F+PG W GI LD+ +GKNDG+V GVRYFQ    RG+F   S+L   R+  + +    A      S  ++   +S T     A++P       S L  ++S S ++   + +      G   L+ G +VL   ++ G++R++G  +F+ G W GVEL    GK+DG V   RYF C P +G+     +++  G
Sbjct:   55 FEIGDRVWVNGNKPGFIQFLGETQFAPGQWAGIVLDEAIGKNDGSVAGVRYFQSEPLRGIFTRPSKLS--RTEGEANGTTTAPPSPTPSTTAAPSVSSTTSTTKKAATPTKPDAPASNLTRANSESMSNLSETGSV---KKGERELKMGDRVLVAGSKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQPKYGLFAPVHKVTRIG 265          

HSP 3 Score: 82.0333 bits (201), Expect = 1.860e-15
Identity = 32/58 (55.17%), Postives = 44/58 (75.86%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVG 1256
            +G++R+ G  +FA G W GVELDEP GKN G V G  YFQC+ +YG+FAPV +V+++G
Sbjct:  208 AGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQPKYGLFAPVHKVTRIG 265          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Sea Lamprey
Match: si:dkeyp-47f9.4 (si:dkeyp-47f9.4 [Source:ZFIN;Acc:ZDB-GENE-131126-9])

HSP 1 Score: 172.94 bits (437), Expect = 6.665e-46
Identity = 98/224 (43.75%), Postives = 132/224 (58.93%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLC--NNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLI 2057
            L++G+R+LV+GQR GV+RF G T F+PG WYGIEL++P G+NDG+V GVRYFQC A  GVFAP SR+  L     + E +      + S+  S M+      LS+  +    S+ Q  L   S      ++++        G   L  G QVL     E+G +R+VG V F+ G W+G+ELR   GKHDG V   RYF C P  G+MVRP R+S +GINGA L+
Sbjct:  437 LELGERLLVSGQRAGVLRFQGTTHFAPGHWYGIELEQPSGRNDGSVAGVRYFQCPAKHGVFAPPSRVQRLTV---SMESLLDLPTGKASHPLSGMSLCFQAALSHWKT----SLNQRKLFQVSKTILYRKWSTSPVDRPVKGPPKLASGNQVLVAGTGEMGTVRFVGEVGFAAGTWLGLELRAPRGKHDGAVGGHRYFSCRPGCGVMVRPGRVSYRGINGARLL 653          

HSP 2 Score: 133.65 bits (335), Expect = 8.870e-33
Identity = 77/195 (39.49%), Postives = 105/195 (53.85%), Query Frame = 3
Query:  168 LCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLLDRGA--DIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNMTTI 740
              FFD     C  +LLS   T+ QLFAVLRQW   VQ  +  I  +M K G + NDRD LTDMT+LHY  K+GA   G+ E A      LL   A    R +WT M  LHYA YFDV   + ++L      ++ A C +   G  LHIAA+ L L  A+ L++  A+  F ++R + P D +P  +++P  M  +
Sbjct:    2 FAFFDPKEPSCQTLLLSPSTTVSQLFAVLRQWVPQVQQHVALIGNEMLKRGCHPNDRDELTDMTLLHYTCKAGAPGIGDAEAAAAFAERLLALEANPSLRSRWTGMNALHYAAYFDVPEIIPVVLQAAGPKEVDATCSDFDFGTALHIAASNLCLSAAKSLLEHSANPLFKNNRGQVPCDVVPDAADMPLEMAEV 196          

HSP 3 Score: 117.087 bits (292), Expect = 2.003e-27
Identity = 70/187 (37.43%), Postives = 102/187 (54.55%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPR--NPLNPQTV----VVDHGTVDISG---------------VAAKVDTGLNKSP-LQIGDRVLVAGQRK-GVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVF 1574
            +G+LR+ G   FA G W G+EL++P G+N G+V GV YFQC  ++GVFAP +RV ++ + +E      T +  +PL+  ++     + H    ++                  + VD  +   P L  G++VLVAG  + G +RFVG   F+ GTW G+EL  P GK+DGAV G RYF C    GV 
Sbjct:  450 AGVLRFQGTTHFAPGHWYGIELEQPSGRNDGSVAGVRYFQCPAKHGVFAPPSRVQRLTVSMESLLDLPTGKASHPLSGMSLCFQAALSHWKTSLNQRKLFQVSKTILYRKWSTSPVDRPVKGPPKLASGNQVLVAGTGEMGTVRFVGEVGFAAGTWLGLELRAPRGKHDGAVGGHRYFSCRPGCGVM 636          

HSP 4 Score: 87.4261 bits (215), Expect = 4.558e-18
Identity = 36/67 (53.73%), Postives = 50/67 (74.63%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL 1592
            L +G RV+V G++ GV+RF GPTEF+   W G+ELD+  GKN+G+V+G RYF C    G+FAPLS++
Sbjct:  234 LTVGQRVIVGGRKTGVLRFCGPTEFAGDQWAGVELDEADGKNNGSVDGTRYFTCAPRHGIFAPLSKV 300          

HSP 5 Score: 79.7221 bits (195), Expect = 9.650e-16
Identity = 31/57 (54.39%), Postives = 45/57 (78.95%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKV 1253
            +G+LR+CGP EFA   WAGVELDE  GKN+G+V+G  YF C  ++G+FAP+++V++ 
Sbjct:  247 TGVLRFCGPTEFAGDQWAGVELDEADGKNNGSVDGTRYFTCAPRHGIFAPLSKVARA 303          

HSP 6 Score: 55.8398 bits (133), Expect = 2.696e-8
Identity = 44/157 (28.03%), Postives = 63/157 (40.13%), Query Frame = 3
Query:  816 PPETGVLSPPIRRQTPGPFPPSHLDLVSISDENEIYEAPTPVSRPVSMWRDEIDNRNLASPSRV---------SAKVTLQAMXXXXXXXXXXXXXXXXSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGI 1259
            P + GV +PP R Q       S LDL +    + +          +S W+  ++ R L   S+               ++    L     ++       G +R+ G V FA G W G+EL  P+GK+ G V G  YF C    GV     RVS  GI
Sbjct:  491 PAKHGVFAPPSRVQRLTVSMESLLDLPTGKASHPLSGMSLCFQAALSHWKTSLNQRKLFQVSKTILYRKWSTSPVDRPVKGPPKLASGNQVLVAGTGEMGTVRFVGEVGFAAGTWLGLELRAPRGKHDGAVGGHRYFSCRPGCGVMVRPGRVSYRGI 647          

HSP 7 Score: 52.7582 bits (125), Expect = 2.188e-7
Identity = 22/61 (36.07%), Postives = 34/61 (55.74%), Query Frame = 3
Query: 1845 VLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRIS 2027
            ++   + G++R+ G   F+   W GVEL    GK++G V   RYF C P HGI    S+++
Sbjct:  241 IVGGRKTGVLRFCGPTEFAGDQWAGVELDEADGKNNGSVDGTRYFTCAPRHGIFAPLSKVA 301          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006020.1 (pep scaffold:Pmarinus_7.0:GL478150:21952:49796:1 gene:ENSPMAG00000005440.1 transcript:ENSPMAT00000006020.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 125.561 bits (314), Expect = 7.361e-30
Identity = 76/237 (32.07%), Postives = 108/237 (45.57%), Query Frame = 3
Query: 1092 LRYCGPVEF--------ADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVE------------------------------------KRPRCVTPRNPLNPQTV--------------------------VVDHGTVDISGVAAKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL 1592
             R  GPV F        A   WAG+EL+EP GKN G+V GV YFQC   +GVF   ++++    E E                                      P+ +TP +P  P+++                           +  G+V     A  +  G  +  L+IGDRVLV+G + GV+RF+G  +F+ G W G+ELD+P+GKNDG+V G RYFQC    G+FAP  ++
Sbjct:   64 FRLDGPVFFWGYGLTSHAVNEWAGIELEEPVGKNDGSVAGVRYFQCRPLHGVFTRPSKLAPTAGEGEAAETDASPNTAAATTATRASSSGATPPSSSSEAATVSSPQVMTPASPATPKSIPTTTTGPPSLPPPTARPPSSTPASMSKMTSGSVSNLSEAGSMKKG--ERELKIGDRVLVSGTKAGVVRFLGEADFAKGEWCGVELDEPLGKNDGSVAGTRYFQCQPKYGLFAPTHKV 298          

HSP 2 Score: 89.7373 bits (221), Expect = 1.149e-18
Identity = 65/218 (29.82%), Postives = 101/218 (46.33%), Query Frame = 3
Query: 1452 GPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTA-----SQTDQLSNASSPNHLS-----------------------MAQSPLMTSSSVS--TTHQFNSPTYFGD-SSGRVFLREGLQVLCN-NELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMV 2009
            G T  +   W GIEL++PVGKNDG+V GVRYFQC    GVF   S+L       +  E   +      +  +   ++     S + + +  SSP  ++                        A+ P  T +S+S  T+   ++ +  G    G   L+ G +VL +  + G++R++G  +F+ G W GVEL    GK+DG V   RYF C P +G+  
Sbjct:   76 GLTSHAVNEWAGIELEEPVGKNDGSVAGVRYFQCRPLHGVFTRPSKLAPTAGEGEAAETDASPNTAAATTATRASSSGATPPSSSSEAATVSSPQVMTPASPATPKSIPTTTTGPPSLPPPTARPPSSTPASMSKMTSGSVSNLSEAGSMKKGERELKIGDRVLVSGTKAGVVRFLGEADFAKGEWCGVELDEPLGKNDGSVAGTRYFQCQPKYGLFA 293          

HSP 3 Score: 82.8037 bits (203), Expect = 1.544e-16
Identity = 31/59 (52.54%), Postives = 44/59 (74.58%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGI 1259
            +G++R+ G  +FA G W GVELDEP GKN G+V G  YFQC+ +YG+FAP  +V+++G 
Sbjct:  245 AGVVRFLGEADFAKGEWCGVELDEPLGKNDGSVAGTRYFQCQPKYGLFAPTHKVTRIGF 303          

HSP 4 Score: 51.2174 bits (121), Expect = 8.024e-7
Identity = 19/39 (48.72%), Postives = 25/39 (64.10%), Query Frame = 3
Query: 1911 WVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRIS 2027
            W G+EL    GK+DG V   RYF C P HG+  RPS+++
Sbjct:   85 WAGIELEEPVGKNDGSVAGVRYFQCRPLHGVFTRPSKLA 123          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006027.1 (pep scaffold:Pmarinus_7.0:GL478150:21952:49796:1 gene:ENSPMAG00000005440.1 transcript:ENSPMAT00000006027.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 124.79 bits (312), Expect = 1.163e-29
Identity = 70/212 (33.02%), Postives = 100/212 (47.17%), Query Frame = 3
Query: 1131 WAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVE------------------------------------KRPRCVTPRNPLNPQTV--------------------------VVDHGTVDISGVAAKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAP 1580
            WAG+EL+EP GKN G+V GV YFQC   +GVF   ++++    E E                                      P+ +TP +P  P+++                           +  G+V     A  +  G  +  L+IGDRVLV+G + GV+RF+G  +F+ G W G+ELD+P+GKNDG+V G RYFQC    G+FAP
Sbjct:   89 WAGIELEEPVGKNDGSVAGVRYFQCRPLHGVFTRPSKLAPTAGEGEAAETDASPNTAAATTATRASSSGATPPSSSSEAATVSSPQVMTPASPATPKSIPTTTTGPPSLPPPTARPPSSTPASMSKMTSGSVSNLSEAGSMKKG--ERELKIGDRVLVSGTKAGVVRFLGEADFAKGEWCGVELDEPLGKNDGSVAGTRYFQCQPKYGLFAP 298          

HSP 2 Score: 89.3521 bits (220), Expect = 1.433e-18
Identity = 63/209 (30.14%), Postives = 98/209 (46.89%), Query Frame = 3
Query: 1479 WYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTA-----SQTDQLSNASSPNHLS-----------------------MAQSPLMTSSSVS--TTHQFNSPTYFGD-SSGRVFLREGLQVLCN-NELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMV 2009
            W GIEL++PVGKNDG+V GVRYFQC    GVF   S+L       +  E   +      +  +   ++     S + + +  SSP  ++                        A+ P  T +S+S  T+   ++ +  G    G   L+ G +VL +  + G++R++G  +F+ G W GVEL    GK+DG V   RYF C P +G+  
Sbjct:   89 WAGIELEEPVGKNDGSVAGVRYFQCRPLHGVFTRPSKLAPTAGEGEAAETDASPNTAAATTATRASSSGATPPSSSSEAATVSSPQVMTPASPATPKSIPTTTTGPPSLPPPTARPPSSTPASMSKMTSGSVSNLSEAGSMKKGERELKIGDRVLVSGTKAGVVRFLGEADFAKGEWCGVELDEPLGKNDGSVAGTRYFQCQPKYGLFA 297          

HSP 3 Score: 82.8037 bits (203), Expect = 1.390e-16
Identity = 31/59 (52.54%), Postives = 44/59 (74.58%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGI 1259
            +G++R+ G  +FA G W GVELDEP GKN G+V G  YFQC+ +YG+FAP  +V+++G 
Sbjct:  249 AGVVRFLGEADFAKGEWCGVELDEPLGKNDGSVAGTRYFQCQPKYGLFAPTHKVTRIGF 307          

HSP 4 Score: 57.3806 bits (137), Expect = 1.219e-8
Identity = 26/62 (41.94%), Postives = 36/62 (58.06%), Query Frame = 3
Query: 1848 LCNNELGIIRYVGAVNFS--DGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRIS 2027
            +CNN  G + Y+   N S  +  W G+EL    GK+DG V   RYF C P HG+  RPS+++
Sbjct:   67 ICNNAQGFVTYL-FFNQSPLEKNWAGIELEEPVGKNDGSVAGVRYFQCRPLHGVFTRPSKLA 127          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004647.1 (pep scaffold:Pmarinus_7.0:GL484285:5077:12891:1 gene:ENSPMAG00000004232.1 transcript:ENSPMAT00000004647.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 110.923 bits (276), Expect = 3.002e-26
Identity = 74/233 (31.76%), Postives = 112/233 (48.07%), Query Frame = 3
Query: 1398 IGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLH--------------ILRSRHKNDEIIKATQQNRLSNHSSMMTASQTD--------------QLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLC-NNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMV 2009
            IG+RV V G + G I+++G T+F+PG W GI LD+  GKNDGAV+GVRYF C A RG+F   S+L                 RS          +  + LS+  + + +S ++                + AS P++L+   S  + + S S + +               L+ G +VL    + G++R+VG  +F+ G W GVEL    GK+DG V   RYF C   +G+  
Sbjct:   30 IGERVWVNGSKPGHIQYLGETQFAPGQWAGIVLDEATGKNDGAVSGVRYFMCEAGRGIFTRPSKLSRRPLAPGVFAGGVGAARSSSTTPLTTPRSSCSALSSRDAGLLSSSSNVAAAAVATTAAAAVAAAVASGPSNLARTGSQSVGNLSESGSAKRAERD----------LKMGDRVLVGGTKAGVVRFVGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQQRYGLFA 252          

HSP 2 Score: 97.4413 bits (241), Expect = 7.723e-22
Identity = 40/67 (59.70%), Postives = 53/67 (79.10%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL 1592
            L++GDRVLV G + GV+RFVG T+F+ G W G+ELD+P+GKNDGAV G RYFQC    G+FAP+ ++
Sbjct:  191 LKMGDRVLVGGTKAGVVRFVGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQQRYGLFAPIHKV 257          

HSP 3 Score: 83.5741 bits (205), Expect = 2.650e-17
Identity = 32/59 (54.24%), Postives = 45/59 (76.27%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGI 1259
            +G++R+ G  +FA G W GVELDEP GKN G V G  YFQC+++YG+FAP+ +V+K+G 
Sbjct:  204 AGVVRFVGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQQRYGLFAPIHKVAKIGF 262          

HSP 4 Score: 60.8474 bits (146), Expect = 4.965e-10
Identity = 26/55 (47.27%), Postives = 36/55 (65.45%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSK 1250
            G ++Y G  +FA G WAG+ LDE  GKN G V+GV YF CE   G+F   +++S+
Sbjct:   42 GHIQYLGETQFAPGQWAGIVLDEATGKNDGAVSGVRYFMCEAGRGIFTRPSKLSR 96          

HSP 5 Score: 60.077 bits (144), Expect = 7.837e-10
Identity = 25/54 (46.30%), Postives = 33/54 (61.11%), Query Frame = 3
Query: 1866 GIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRIS 2027
            G I+Y+G   F+ G W G+ L   TGK+DG V   RYF+C    GI  RPS++S
Sbjct:   42 GHIQYLGETQFAPGQWAGIVLDEATGKNDGAVSGVRYFMCEAGRGIFTRPSKLS 95          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Sea Lamprey
Match: dctn1a (dynactin 1a [Source:ZFIN;Acc:ZDB-GENE-070117-2205])

HSP 1 Score: 73.9442 bits (180), Expect = 1.049e-13
Identity = 32/72 (44.44%), Postives = 46/72 (63.89%), Query Frame = 3
Query: 1389 PLQIGDRVLVAGQRK-GVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHIL 1601
            PL++G RV V G+ + G + +VG T F+ G W G+ LD+  GKNDG V G RYF C  N G+F   S++ ++
Sbjct:   24 PLKVGSRVEVIGKGQIGTVAYVGATMFATGKWVGVILDEAKGKNDGTVQGKRYFTCEDNHGIFVRQSQIQLV 95          

HSP 2 Score: 70.4774 bits (171), Expect = 1.042e-12
Identity = 33/76 (43.42%), Postives = 46/76 (60.53%), Query Frame = 3
Query: 1839 LQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLIPPE 2066
            ++V+   ++G + YVGA  F+ G WVGV L    GK+DG V  KRYF C  +HGI VR S+I +   +G +   PE
Sbjct:   31 VEVIGKGQIGTVAYVGATMFATGKWVGVILDEAKGKNDGTVQGKRYFTCEDNHGIFVRQSQIQLVE-DGGDTTSPE 105          

HSP 3 Score: 62.3882 bits (150), Expect = 3.043e-10
Identity = 31/72 (43.06%), Postives = 41/72 (56.94%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNP 1301
            G + Y G   FA G W GV LDE +GKN GTV G  YF CE+ +G+F    R S++ + VE      +P +P
Sbjct:   40 GTVAYVGATMFATGKWVGVILDEAKGKNDGTVQGKRYFTCEDNHGIF---VRQSQIQL-VEDGGDTTSPESP 107          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Yeast
Match: NIP100 (Large subunit of the dynactin complex; dynactin is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) [Source:SGD;Acc:S000006095])

HSP 1 Score: 83.5741 bits (205), Expect = 5.132e-17
Identity = 36/70 (51.43%), Postives = 48/70 (68.57%), Query Frame = 3
Query: 1359 AKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRG 1568
             +VDT + K  LQ  D VLV  + KG ++F+G T+F+ G WYGIELDKP+GKNDG+ NG+RYF     + 
Sbjct:    6 VQVDTNMQKISLQ--DTVLV-NEMKGRVKFIGETQFAKGIWYGIELDKPLGKNDGSANGIRYFDIDLKKA 72          

HSP 2 Score: 56.225 bits (134), Expect = 1.694e-8
Identity = 22/55 (40.00%), Postives = 34/55 (61.82%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQ--------YGVF 1226
            G +++ G  +FA G+W G+ELD+P GKN G+ NG+ YF  + +        YG+F
Sbjct:   28 GRVKFIGETQFAKGIWYGIELDKPLGKNDGSANGIRYFDIDLKKANSNGGYYGLF 82          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Yeast
Match: BIK1 (Microtubule-associated protein; component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 [Source:SGD;Acc:S000000534])

HSP 1 Score: 46.9802 bits (110), Expect = 8.920e-6
Identity = 32/105 (30.48%), Postives = 55/105 (52.38%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEE-QYGVFAPVTRVSKVGIEVEKRPRCVTP-RNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPL 1394
            G L+Y GPV+   G++AGV+L    GKN G+  G  YFQ E  Q G+F  + +V+ +   +EK     T  R  + P ++  +   V ++   + +D   + +P+
Sbjct:   20 GQLKYVGPVDTKAGMFAGVDLLANIGKNDGSFMGKKYFQTEYPQSGLFIQLQKVASL---IEKASISQTSRRTTMEPLSIPKNRSIVRLTNQFSPMDDPKSPTPM 121          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Nematostella
Match: EDO43343 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZ45])

HSP 1 Score: 181.415 bits (459), Expect = 2.944e-50
Identity = 149/500 (29.80%), Postives = 208/500 (41.60%), Query Frame = 3
Query:  168 LCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQGEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYF------DVAPA---VELLLDRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNMTTIRNNLGTLLCPTSPPGHTYYNANHRKPPETGVLSPPIRRQTPGPFPPSHLD-LVSISDENEIYEAPTPVSRPVSMWRDEIDNRNLASPSRVSAKVTLQAMXXXXXXXXXXXXXXXXSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVE-KRPRCVTPRNPLNPQTVVVDH--------------GTVDISGVAAKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL 1592
            L FFD +C  C  VL     TI Q+FA++RQW   VQ  +E  I++                                         LL  GA L  R   TDM  LHYA         D+A A   +  L+ +GAD+   CR       LH A    A    R+L+K    +   D   R    C    +    ++           C        Y      + P   V  P +    P   P    + +  +  E E  EAP P          E +N++ A+   +  +V +                   SG+LR+CG  EFA G W GVELD+P GKN G+V+GV YFQCE  +G+FAP ++V++ G      R +    R P    T+V                         + ++D        QIG+ VLVAGQ+ G+++F+G T+F+ G W GIEL  PVGKNDG+V GVRYF C    GVFAP++++
Sbjct:    3 LTFFDPNCPSCYDVLQLPTTTIPQVFAIMRQWVPQVQQNIELFIQE-----------------------------------------LLRRGAHLNDRDGLTDMCVLHYACKAGAVGVGDIAAASNVISGLIAKGADVSQKCR-WTDMLPLHYATFFDAAPVIRILLKESHSK---DVNTR----CKEFEAGTALHIAATNLCFEAARCLLQHGADAYLRDKLNRTPRECV--PDVSAYDPESEPAIVAEKMTRLLSEAEPLEAPKP----------ETENQD-ANELTIGERVYIGGTK---------------SGVLRFCGRTEFASGEWVGVELDQPVGKNDGSVDGVQYFQCEPNHGIFAPASKVTRNGTTQHISRLKPRASRRPPGESTLVTSQPNSRPSSRPSSRPNSRSPSPTPSPRLDDSEIARDFQIGEHVLVAGQKPGIVQFIGQTQFASGWWLGIELSNPVGKNDGSVGGVRYFTCKPRFGVFAPVAKV 425          

HSP 2 Score: 125.176 bits (313), Expect = 9.266e-31
Identity = 75/224 (33.48%), Postives = 114/224 (50.89%), Query Frame = 3
Query: 1380 NKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL-------HILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNEL-GIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRIS 2027
            + + L IG+RV + G + GV+RF G TEF+ G W G+ELD+PVGKNDG+V+GV+YFQC  N G+FAP S++       HI R        +K     R    S+++T+               +   S   +S   S +          DS      + G  VL   +  GI++++G   F+ G W+G+EL +  GK+DG V   RYF C P  G+    ++++
Sbjct:  224 DANELTIGERVYIGGTKSGVLRFCGRTEFASGEWVGVELDQPVGKNDGSVDGVQYFQCEPNHGIFAPASKVTRNGTTQHISR--------LKPRASRRPPGESTLVTS-------------QPNSRPSSRPSSRPNSRSPSPTPSPRLDDSEIARDFQIGEHVLVAGQKPGIVQFIGQTQFASGWWLGIELSNPVGKNDGSVGGVRYFTCKPRFGVFAPVAKVT 426          

HSP 3 Score: 70.0922 bits (170), Expect = 1.058e-12
Identity = 29/55 (52.73%), Postives = 40/55 (72.73%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSK 1250
            GI+++ G  +FA G W G+EL  P GKN G+V GV YF C+ ++GVFAPV +V+K
Sbjct:  373 GIVQFIGQTQFASGWWLGIELSNPVGKNDGSVGGVRYFTCKPRFGVFAPVAKVTK 427          

HSP 4 Score: 59.6918 bits (143), Expect = 2.398e-9
Identity = 25/57 (43.86%), Postives = 35/57 (61.40%), Query Frame = 3
Query: 1866 GIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKG 2036
            G++R+ G   F+ G WVGVEL    GK+DG V   +YF C P+HGI    S+++  G
Sbjct:  242 GVLRFCGRTEFASGEWVGVELDQPVGKNDGSVDGVQYFQCEPNHGIFAPASKVTRNG 298          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Nematostella
Match: EDO33600 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRR5])

HSP 1 Score: 142.51 bits (358), Expect = 2.083e-37
Identity = 69/169 (40.83%), Postives = 100/169 (59.17%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL 1592
            G++ + G  +FA G WAG+ LD+P GKN G+V G  YF+C+  +GVF  + +++KV    E      +P +P+                         N S L+IGDRV+V+G + G +R+VG TEF+ G W G+ELD+P+GKNDGAV G RYFQC    G+FAP+ ++
Sbjct:   21 GVIAFLGETKFAKGDWAGIILDDPTGKNDGSVAGEKYFECKPLHGVFTKLEKITKVNQVTE------SPGSPVE------------------------NSSSLEIGDRVVVSGNKIGTLRYVGTTEFAKGEWAGVELDEPLGKNDGAVAGTRYFQCIQGYGLFAPVHKV 159          

HSP 2 Score: 112.849 bits (281), Expect = 3.449e-27
Identity = 72/205 (35.12%), Postives = 96/205 (46.83%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIM 2006
             Q+GDRVLV   + GVI F+G T+F+ G W GI LD P GKNDG+V G +YF+C    GVF  L ++               T+ N+++                  SP       SP+  SSS+            GD   RV       V+  N++G +RYVG   F+ G W GVEL    GK+DG V   RYF C   +G+ 
Sbjct:    7 FQVGDRVLVNNTKPGVIAFLGETKFAKGDWAGIILDDPTGKNDGSVAGEKYFECKPLHGVFTKLEKI---------------TKVNQVTE-----------------SPG------SPVENSSSLE----------IGD---RV-------VVSGNKIGTLRYVGTTEFAKGEWAGVELDEPLGKNDGAVAGTRYFQCIQGYGLF 153          

HSP 3 Score: 58.5362 bits (140), Expect = 3.798e-9
Identity = 26/65 (40.00%), Postives = 39/65 (60.00%), Query Frame = 3
Query: 1836 GLQVLCNNEL-GIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRIS 2027
            G +VL NN   G+I ++G   F+ G W G+ L   TGK+DG V  ++YF C P HG+  +  +I+
Sbjct:   10 GDRVLVNNTKPGVIAFLGETKFAKGDWAGIILDDPTGKNDGSVAGEKYFECKPLHGVFTKLEKIT 74          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Nematostella
Match: EDO30218 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T1F2])

HSP 1 Score: 73.559 bits (179), Expect = 1.696e-16
Identity = 33/57 (57.89%), Postives = 40/57 (70.18%), Query Frame = 3
Query: 1428 RKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHI 1598
            + GVIRF+G TEFSPG W G+ELDK  GKNDG+V+GVRYF C    G F    ++ I
Sbjct:    1 KTGVIRFIGATEFSPGPWVGVELDKAGGKNDGSVSGVRYFACKPRFGSFVRPDKVKI 57          

HSP 2 Score: 63.1586 bits (152), Expect = 7.282e-13
Identity = 28/55 (50.91%), Postives = 35/55 (63.64%), Query Frame = 3
Query: 1866 GIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISV 2030
            G+IR++GA  FS G WVGVEL    GK+DG V   RYF C P  G  VRP ++ +
Sbjct:    3 GVIRFIGATEFSPGPWVGVELDKAGGKNDGSVSGVRYFACKPRFGSFVRPDKVKI 57          

HSP 3 Score: 60.077 bits (144), Expect = 1.059e-11
Identity = 28/59 (47.46%), Postives = 39/59 (66.10%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGI 1259
            +G++R+ G  EF+ G W GVELD+  GKN G+V+GV YF C+ ++G F    R  KV I
Sbjct:    2 TGVIRFIGATEFSPGPWVGVELDKAGGKNDGSVSGVRYFACKPRFGSF---VRPDKVKI 57          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Nematostella
Match: EDO44433 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVR2])

HSP 1 Score: 77.7962 bits (190), Expect = 1.298e-14
Identity = 36/71 (50.70%), Postives = 43/71 (60.56%), Query Frame = 3
Query: 1398 IGDRV---LVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHIL 1601
            +GDRV   L    R GV+RF G T FSPG W GI LD   GKNDG+V GVRYF C  + G+F    ++  L
Sbjct:  202 LGDRVVCVLNKRLRTGVLRFTGTTHFSPGEWCGIVLDDACGKNDGSVKGVRYFDCKQDHGIFVHAHKVRAL 272          

HSP 2 Score: 61.6178 bits (148), Expect = 9.965e-10
Identity = 23/54 (42.59%), Postives = 35/54 (64.81%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRV 1244
            +G+LR+ G   F+ G W G+ LD+  GKN G+V GV YF C++ +G+F    +V
Sbjct:  216 TGVLRFTGTTHFSPGEWCGIVLDDACGKNDGSVKGVRYFDCKQDHGIFVHAHKV 269          

HSP 3 Score: 57.3806 bits (137), Expect = 2.205e-8
Identity = 28/67 (41.79%), Postives = 38/67 (56.72%), Query Frame = 3
Query: 1836 GLQVLC--NNEL--GIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRI 2024
            G +V+C  N  L  G++R+ G  +FS G W G+ L    GK+DG V   RYF C  DHGI V   ++
Sbjct:  203 GDRVVCVLNKRLRTGVLRFTGTTHFSPGEWCGIVLDDACGKNDGSVKGVRYFDCKQDHGIFVHAHKV 269          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Nematostella
Match: EDO48890 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RHP8])

HSP 1 Score: 70.4774 bits (171), Expect = 1.076e-12
Identity = 30/62 (48.39%), Postives = 42/62 (67.74%), Query Frame = 3
Query: 1398 IGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPL 1583
            +GD V+V G+ KG+I+++GP +    ++ G+ LD PVG NDG +NGVRYF C  N G F  L
Sbjct:  358 LGDYVVVQGEIKGIIKYLGPIKGELSSYAGVHLDSPVGNNDGTLNGVRYFFCPQNYGAFVLL 419          

HSP 2 Score: 56.6102 bits (135), Expect = 2.352e-8
Identity = 24/47 (51.06%), Postives = 31/47 (65.96%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVF 1226
            GI++Y GP++     +AGV LD P G N GT+NGV YF C + YG F
Sbjct:  370 GIIKYLGPIKGELSSYAGVHLDSPVGNNDGTLNGVRYFFCPQNYGAF 416          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Medaka
Match: si:dkeyp-47f9.4 (CAP-Gly domain containing linker protein family member 4 [Source:NCBI gene;Acc:101171960])

HSP 1 Score: 208.764 bits (530), Expect = 2.090e-57
Identity = 137/427 (32.08%), Postives = 205/427 (48.01%), Query Frame = 3
Query:  120 HPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLL--DRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNM---TTIRNNLGTLLCPTSPPGHTYYNANHRKPPETGVLSPPIRRQTPGPFPPSHLDLVSISDENEIYEAPTPVSRPVSMWRDEIDNRNLASPSRVSAKVTLQAMXXXXXXXXXXXXXXXXSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGL 1379
            H    AP+  +C      FF+     C ++L  +  TI +LFAVLRQW   VQ  +  I  ++ K G  VNDRDGLTDM++LHY  K+GA   G+ E+A      LL  GA    R +WT+M  LHYA YFDV   + ++L   R  DI A C +   G  LHIAA+ L + T + L++ GA+  F +++ + P D +P   ++P  M     +   L  LL  T P                       R  +P PF      + ++SD+ ++  A   +                    R+  +V +    +               G LR+CG  EF+ G+WAGVELD+ +GKN+G V GV YF C  ++G+FAP++++SK    +EKR          N  T+    G +D S  ++K++TG+
Sbjct:   31 HQVASAPMPSDCE---FSFFNPSEPQCKEILQDSNTTIPELFAVLRQWVPQVQKNIHLIGSEILKRGCGVNDRDGLTDMSLLHYCCKAGAPGIGDAEIAASFARQLLALGADANLRSRWTNMRALHYAAYFDVPELIRVILQASRPGDIDATCSDFDFGTALHIAASNLCISTVKCLLELGANHTFKNEKGQCPVDVVPDPLDMPLEMADAAAVAKELRVLLRRTLP-----------------------RPSSPLPFIIQPQSIPALSDKAKMQLASMGI--------------------RMGDRVVIAGQKV---------------GTLRFCGSTEFSGGLWAGVELDKAEGKNNGAVAGVQYFTCRMKHGIFAPLSKISK---PLEKRKTSSVK----NSTTIRPSRG-IDSSRTSSKINTGI 388          

HSP 2 Score: 175.637 bits (444), Expect = 7.844e-46
Identity = 98/236 (41.53%), Postives = 139/236 (58.90%), Query Frame = 3
Query: 1386 SPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGD----------SSGRVFLREGLQVLCN--NELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLI 2057
            S L++G+RVLV GQR GV++F G T F+PG W GIELDKP GKNDG+V GVRYF+C    GVFAP S +  +   H + + +     +R S H    T  ++   S A +    S  +SP+  + S     + N  +              S G+V LR G+QV  N  N++  IRY G  +F+ G+W+G+ELR   GK+DG V  +RYF C P +G++VRPSR++ +GING+ L+
Sbjct:  465 SELRLGERVLVVGQRTGVVQFCGKTSFAPGIWLGIELDKPNGKNDGSVGGVRYFRCPPKHGVFAPPSYVQRI---HGSVDCLSELSSSRFS-HPFKGTIRRSFSTSTAIAALKESNRKSPINRNHSNPLRRRSNHLSGGSGGISRGSSPLLSDGQVRLRLGMQVFLNSANDMAFIRYFGTADFAPGLWLGLELRSPKGKNDGSVGGRRYFTCRPGYGVLVRPSRVTYRGINGSRLV 696          

HSP 3 Score: 135.576 bits (340), Expect = 1.203e-32
Identity = 92/265 (34.72%), Postives = 131/265 (49.43%), Query Frame = 3
Query: 1344 ISGVAAKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQ----------------------------------TDQLSNASSPNHLSMAQSPLMTSSSVSTTHQ-FNSPTYFGDSS--GRVFLREGLQVL-CNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRI 2024
            I  ++ K    L    +++GDRV++AGQ+ G +RF G TEFS G W G+ELDK  GKN+GAV GV+YF C    G+FAPLS++     + K   +  +T         S  T+S+                                  T Q  +A+S N  + + + L +S + STT   F S T  G SS      LR G +VL      G++++ G  +F+ GIW+G+EL    GK+DG V   RYF CPP HG+   PS +
Sbjct:  269 IPALSDKAKMQLASMGIRMGDRVVIAGQKVGTLRFCGSTEFSGGLWAGVELDKAEGKNNGAVAGVQYFTCRMKHGIFAPLSKISKPLEKRKTSSVKNSTTIRPSRGIDSSRTSSKINTGILSRSSSSSSLDSRRGPDARPRPLPRRRISTRQRKDATSQNPRTASTTALRSSRTTSTTTAGFRSRTPSGSSSLCDGSELRLGERVLVVGQRTGVVQFCGKTSFAPGIWLGIELDKPNGKNDGSVGGVRYFRCPPKHGVFAPPSYV 533          

HSP 4 Score: 107.457 bits (267), Expect = 1.189e-23
Identity = 65/208 (31.25%), Postives = 99/208 (47.60%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSP-------------------------------------LQIGDRVLV-AGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL 1592
            +G++++CG   FA G+W G+ELD+P GKN G+V GV YF+C  ++GVFAP + V ++   V+      + R   +P    +       + +AA  ++   KSP                                     L++G +V + +      IR+ G  +F+PG W G+EL  P GKNDG+V G RYF C    GV    SR+
Sbjct:  480 TGVVQFCGKTSFAPGIWLGIELDKPNGKNDGSVGGVRYFRCPPKHGVFAPPSYVQRIHGSVDCLSELSSSRFS-HPFKGTIRRSFSTSTAIAALKESN-RKSPINRNHSNPLRRRSNHLSGGSGGISRGSSPLLSDGQVRLRLGMQVFLNSANDMAFIRYFGTADFAPGLWLGLELRSPKGKNDGSVGGRRYFTCRPGYGVLVRPSRV 685          

HSP 5 Score: 61.2326 bits (147), Expect = 3.149e-9
Identity = 27/57 (47.37%), Postives = 35/57 (61.40%), Query Frame = 3
Query: 1089 ILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGI 1259
             +RY G  +FA G+W G+EL  P+GKN G+V G  YF C   YGV    +RV+  GI
Sbjct:  634 FIRYFGTADFAPGLWLGLELRSPKGKNDGSVGGRRYFTCRPGYGVLVRPSRVTYRGI 690          

HSP 6 Score: 57.7658 bits (138), Expect = 3.925e-8
Identity = 25/61 (40.98%), Postives = 36/61 (59.02%), Query Frame = 3
Query: 1845 VLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRIS 2027
            V+   ++G +R+ G+  FS G+W GVEL    GK++G V   +YF C   HGI    S+IS
Sbjct:  292 VIAGQKVGTLRFCGSTEFSGGLWAGVELDKAEGKNNGAVAGVQYFTCRMKHGIFAPLSKIS 352          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Medaka
Match: clip3 (CAP-Gly domain containing linker protein 3 [Source:NCBI gene;Acc:101171507])

HSP 1 Score: 170.629 bits (431), Expect = 4.160e-45
Identity = 91/210 (43.33%), Postives = 129/210 (61.43%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDIS-------------GVAAKVD-TGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHIL---RSRHKNDEIIKATQQNRLSN 1661
            +G LR+CG  EFA G W GVELDEP+GKN G+V GV YF C  + G+FAPV+++SK    V++ P  VT   P  P+  +    T  ++              +AA +D  G N   +++GD+VLVAGQ+ G++RF G T+F+PG W+GIELD+P GK+DG+V GVRYF C    GVFAP SR+  +   +   +N+ ++K   Q  +S 
Sbjct:  284 TGSLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKLGIFAPVSKISKA---VDQMPSSVT-STPRTPRIDLASRLTPSLARPLKDRRRSQKKKSLAANLDPEGTN---VEVGDQVLVAGQKNGIVRFYGKTDFAPGYWFGIELDQPTGKHDGSVFGVRYFSCLPKYGVFAPPSRVQRIGGPKDGSQNNSMVKKVHQVTMSQ 486          

HSP 2 Score: 159.458 bits (402), Expect = 2.765e-41
Identity = 80/209 (38.28%), Postives = 125/209 (59.81%), Query Frame = 3
Query:   90 ISERRKYPETHPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLLDRGAD--IRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLP 704
            + E R+    HP+ +APL     +Y   FFD +   C+++L+  R TI +LFA++RQW   VQ +++ I +++ K G +VNDRDGLTDMT+LHY+ K+GA   G+   A+R+ A L+  GA +  R +WT+M  LHYA YFDV   + +LL       + + C +   G  LHIAA  L L + + L++ GA+    +D+ + P + +P
Sbjct:   11 VHEPRRRAMVHPSAQAPLP---KDYAFTFFDPNDPACLEILMDPRTTIPELFAIVRQWVPQVQHKIDIIGKEILKRGCHVNDRDGLTDMTLLHYSCKAGAHGVGDPAAALRLSAQLISLGADVSLRSRWTNMNALHYAAYFDVPELIRVLLKAAKPRVLNSTCSDFHHGTALHIAACNLCLGSVKCLLEHGANPTVRNDKGQVPAEVVP 216          

HSP 3 Score: 129.413 bits (324), Expect = 3.494e-31
Identity = 77/219 (35.16%), Postives = 112/219 (51.14%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSN---ASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNEL-GIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKG 2036
            L++GDRV++   + G +RF G TEF+ G W G+ELD+P GKNDG+V GVRYF C    G+FAP+S+  I ++  +    + +T +    + +S +T S    L +   +     L+    P  T+  V                       G QVL   +  GI+R+ G  +F+ G W G+EL   TGKHDG V   RYF C P +G+   PSR+   G
Sbjct:  271 LKLGDRVVLDDNKTGSLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKLGIFAPVSK--ISKAVDQMPSSVTSTPRTPRIDLASRLTPSLARPLKDRRRSQKKKSLAANLDPEGTNVEV-----------------------GDQVLVAGQKNGIVRFYGKTDFAPGYWFGIELDQPTGKHDGSVFGVRYFSCLPKYGVFAPPSRVQRIG 464          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Medaka
Match: clip3 (CAP-Gly domain containing linker protein 3 [Source:NCBI gene;Acc:101171507])

HSP 1 Score: 168.703 bits (426), Expect = 6.829e-44
Identity = 91/210 (43.33%), Postives = 126/210 (60.00%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVT--PRNP------------LNPQTVVVDHGTVDISGVAAKVD-TGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHIL---RSRHKNDEIIKATQQNRLS 1658
            +G LR+CG  EFA G W GVELDEP+GKN G+V GV YF C  + G+FAPV+++SK    V++ P  VT  PR P               +             +AA +D  G N   +++GD+VLVAGQ+ G++RF G T+F+PG W+GIELD+P GK+DG+V GVRYF C    GVFAP SR+  +   +   +N+ ++K   Q  +S
Sbjct:  395 TGSLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKLGIFAPVSKISKA---VDQMPSSVTSTPRTPRIDLASRLAGKTKKEKEKKEKERAQKKKSLAANLDPEGTN---VEVGDQVLVAGQKNGIVRFYGKTDFAPGYWFGIELDQPTGKHDGSVFGVRYFSCLPKYGVFAPPSRVQRIGGPKDGSQNNSMVKKVHQVTMS 598          

HSP 2 Score: 159.844 bits (403), Expect = 7.569e-41
Identity = 80/209 (38.28%), Postives = 125/209 (59.81%), Query Frame = 3
Query:   90 ISERRKYPETHPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLLDRGAD--IRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLP 704
            + E R+    HP+ +APL     +Y   FFD +   C+++L+  R TI +LFA++RQW   VQ +++ I +++ K G +VNDRDGLTDMT+LHY+ K+GA   G+   A+R+ A L+  GA +  R +WT+M  LHYA YFDV   + +LL       + + C +   G  LHIAA  L L + + L++ GA+    +D+ + P + +P
Sbjct:  122 VHEPRRRAMVHPSAQAPLP---KDYAFTFFDPNDPACLEILMDPRTTIPELFAIVRQWVPQVQHKIDIIGKEILKRGCHVNDRDGLTDMTLLHYSCKAGAHGVGDPAAALRLSAQLISLGADVSLRSRWTNMNALHYAAYFDVPELIRVLLKAAKPRVLNSTCSDFHHGTALHIAACNLCLGSVKCLLEHGANPTVRNDKGQVPAEVVP 327          

HSP 3 Score: 129.028 bits (323), Expect = 1.171e-30
Identity = 80/216 (37.04%), Postives = 110/216 (50.93%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNEL-GIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKG 2036
            L++GDRV++   + G +RF G TEF+ G W G+ELD+P GKNDG+V GVRYF C    G+FAP+S+            I KA  Q      SS+ +  +T ++  AS    L+                +  S     D  G   +  G QVL   +  GI+R+ G  +F+ G W G+EL   TGKHDG V   RYF C P +G+   PSR+   G
Sbjct:  382 LKLGDRVVLDDNKTGSLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKLGIFAPVSK------------ISKAVDQMP----SSVTSTPRTPRIDLAS---RLAGKTKKEKEKKEKERAQKKKSLAANLDPEG-TNVEVGDQVLVAGQKNGIVRFYGKTDFAPGYWFGIELDQPTGKHDGSVFGVRYFSCLPKYGVFAPPSRVQRIG 577          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Medaka
Match: clip3 (CAP-Gly domain containing linker protein 3 [Source:NCBI gene;Acc:101171507])

HSP 1 Score: 167.933 bits (424), Expect = 1.080e-43
Identity = 91/211 (43.13%), Postives = 126/211 (59.72%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVT--PRNP------------LNPQTVVVDHGTVDISGVAAKVD-TGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHIL---RSRHKNDEIIKATQQNRLSN 1661
            +G LR+CG  EFA G W GVELDEP+GKN G+V GV YF C  + G+FAPV+++SK    V++ P  VT  PR P               +             +AA +D  G N   +++GD+VLVAGQ+ G++RF G T+F+PG W+GIELD+P GK+DG+V GVRYF C    GVFAP SR+  +   +   +N+ ++K   Q  +S 
Sbjct:  370 TGSLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKLGIFAPVSKISKA---VDQMPSSVTSTPRTPRIDLASRLAGKTKKEKEKKEKERAQKKKSLAANLDPEGTN---VEVGDQVLVAGQKNGIVRFYGKTDFAPGYWFGIELDQPTGKHDGSVFGVRYFSCLPKYGVFAPPSRVQRIGGPKDGSQNNSMVKKVHQVTMSQ 574          

HSP 2 Score: 158.688 bits (400), Expect = 1.353e-40
Identity = 82/210 (39.05%), Postives = 127/210 (60.48%), Query Frame = 3
Query:   90 ISERRKYPETHPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQ--GEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLLDRGADIRAI---CRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLP 704
            + E R+    HP+ +APL     +Y   FFD +   C+++L+  R TI +LFA++RQW   VQ +++ I +++ K G +VNDRDGLTDMT+LHY+ K+GA   G+   A+R+ A L+  GA +  R +WT+M  LHYA YFDV   + +LL + A  R +   C +   G  LHIAA  L L + + L++ GA+    +D+ + P + +P
Sbjct:   97 VHEPRRRAMVHPSAQAPLP---KDYAFTFFDPNDPACLEILMDPRTTIPELFAIVRQWVPQVQHKIDIIGKEILKRGCHVNDRDGLTDMTLLHYSCKAGAHGVGDPAAALRLSAQLISLGADVSLRSRWTNMNALHYAAYFDVPELIRVLL-KAAKPRVLNSTCSDFHHGTALHIAACNLCLGSVKCLLEHGANPTVRNDKGQVPAEVVP 302          

HSP 3 Score: 129.028 bits (323), Expect = 1.020e-30
Identity = 80/216 (37.04%), Postives = 110/216 (50.93%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNEL-GIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKG 2036
            L++GDRV++   + G +RF G TEF+ G W G+ELD+P GKNDG+V GVRYF C    G+FAP+S+            I KA  Q      SS+ +  +T ++  AS    L+                +  S     D  G   +  G QVL   +  GI+R+ G  +F+ G W G+EL   TGKHDG V   RYF C P +G+   PSR+   G
Sbjct:  357 LKLGDRVVLDDNKTGSLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKLGIFAPVSK------------ISKAVDQMP----SSVTSTPRTPRIDLAS---RLAGKTKKEKEKKEKERAQKKKSLAANLDPEG-TNVEVGDQVLVAGQKNGIVRFYGKTDFAPGYWFGIELDQPTGKHDGSVFGVRYFSCLPKYGVFAPPSRVQRIG 552          
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Medaka
Match: ENSORLT00000001833.2 (pep primary_assembly:ASM223467v1:12:2504412:2509132:-1 gene:ENSORLG00000001483.2 transcript:ENSORLT00000001833.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 141.354 bits (355), Expect = 5.972e-37
Identity = 74/176 (42.05%), Postives = 105/176 (59.66%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGI----EVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVD-TGLNKS--PLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL 1592
            G + + G  +FA G WAG+ LDEP GKN G+V GV YFQCE+  G+F   +++S+  +    E  +R  CV        +   +          +   D   L KS   L++GDRVLV+G + GV+RF+G T+F+ G W G+ELD+P+GKNDGAV G RYFQC    G+FAP+ ++
Sbjct:   55 GYVHFVGGTQFAPGQWAGIVLDEPIGKNDGSVAGVRYFQCEDGRGIFTRPSKLSRTALPEKEESRRRRFCVCSAQGTGIKMSALTRMAASSESASNLSDPDSLKKSRRELRLGDRVLVSGSKAGVVRFLGETDFAKGDWCGVELDEPLGKNDGAVAGARYFQCLPRFGLFAPVHKV 230          

HSP 2 Score: 128.642 bits (322), Expect = 1.744e-32
Identity = 74/212 (34.91%), Postives = 105/212 (49.53%), Query Frame = 3
Query: 1392 LQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDE-----IIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLCN-NELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMV 2009
             Q GDRV V G + G + FVG T+F+PG W GI LD+P+GKNDG+V GVRYFQC   RG+F   S+L       K +       + + Q   +   +    A+ ++  SN S P+ L  +                           R  LR G +VL + ++ G++R++G  +F+ G W GVEL    GK+DG V   RYF C P  G+  
Sbjct:   41 FQTGDRVWVNGNKPGYVHFVGGTQFAPGQWAGIVLDEPIGKNDGSVAGVRYFQCEDGRGIFTRPSKLSRTALPEKEESRRRRFCVCSAQGTGIKMSALTRMAASSESASNLSDPDSLKKS---------------------------RRELRLGDRVLVSGSKAGVVRFLGETDFAKGDWCGVELDEPLGKNDGAVAGARYFQCLPRFGLFA 225          

HSP 3 Score: 77.411 bits (189), Expect = 4.590e-15
Identity = 34/70 (48.57%), Postives = 46/70 (65.71%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTP 1292
            +G++R+ G  +FA G W GVELDEP GKN G V G  YFQC  ++G+FAPV +V+++G        C TP
Sbjct:  177 AGVVRFLGETDFAKGDWCGVELDEPLGKNDGAVAGARYFQCLPRFGLFAPVHKVTRIGFP------CTTP 240          
BLAST of CAP-Gly domain-containing linker protein vs. Planmine SMEST
Match: SMESG000015465.1 (SMESG000015465.1)

HSP 1 Score: 1347.8 bits (3487), Expect = 0.000e+0
Identity = 667/671 (99.40%), Postives = 669/671 (99.70%), Query Frame = 3
Query:   45 MKMTSQDVLVMSVDTISERRKYPETHPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQGEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLLDRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNMTTIRNNLGTLLCPTSPPGHTYYNANHRKPPETGVLSPPIRRQTPGPFPPSHLDLVSISDENEIYEAPTPVSRPVSMWRDEIDNRNLASPSRVSAKVTLQAMXXXXXXXXXXXXXXXXSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLI 2057
            MKMTSQDVLVMSVDTISERRKYPETHPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQGEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLLDRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNMTTIRNNLGTLLCPTSPPGHTYYNANHRKPPETGVLSPPIRRQTPGPFPPSHLDLVSISDENEIYEAPTPVSRPVSMWRDEIDNRNLASPSRVSAKVTLQAMGLLLGDRVLVRGDVDRSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQI DRVLVAGQRKGVIRFVGPT+FSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLH LRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSS+VSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLI
Sbjct:    1 MKMTSQDVLVMSVDTISERRKYPETHPAVEAPLCDNCAEYGLCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKLGANVNDRDGLTDMTMLHYAAKSGAQGEEELAVRVIAYLLDEGALLEARCKWTDMTPLHYAVYFDVAPAVELLLDRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNMTTIRNNLGTLLCPTSPPGHTYYNANHRKPPETGVLSPPIRRQTPGPFPPSHLDLVSISDENEIYEAPTPVSRPVSMWRDEIDNRNLASPSRVSAKVTLQAMGLLLGDRVLVRGDVDRSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQICDRVLVAGQRKGVIRFVGPTKFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHKLRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSAVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLI 671          
BLAST of CAP-Gly domain-containing linker protein vs. Planmine SMEST
Match: SMESG000015470.1 (SMESG000015470.1)

HSP 1 Score: 1080.47 bits (2793), Expect = 0.000e+0
Identity = 540/544 (99.26%), Postives = 542/544 (99.63%), Query Frame = 3
Query:  477 MTPLHYAVYFDVAPAVELLLDRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNMTTIRNNLGTLLCPTSPPGHTYYNANHRKPPETGVLSPPIRRQTPGPFPPSHLDLVSISDENEIYEAPTPVSRPVSMWRDEIDNRNLASPSRVSAKVTLQAMXXXXXXXXXXXXXXXXSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLIPPELAYLEKKILRNSPM 2108
            MTPLHYAVYFDVAPAVELLLDRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNMTTIRNNLGTLLCPTSPPGHTYYNANHRKPPETGVLSPPIRRQTPGPFPPSHLDLVSISDENEIYEAPTPVSRPVSMWRDEIDNRNLASPSRVSAKVTLQAMGLLLGDRVLVRGDVDRSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQI DRVLVAGQRKGVIRFVGPT+FSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLH LRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSS+VSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLIPPELAYLEKKILRNSPM
Sbjct:    1 MTPLHYAVYFDVAPAVELLLDRGADIRAICRELCRGNCLHIAATQLALDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNMTTIRNNLGTLLCPTSPPGHTYYNANHRKPPETGVLSPPIRRQTPGPFPPSHLDLVSISDENEIYEAPTPVSRPVSMWRDEIDNRNLASPSRVSAKVTLQAMGLLLGDRVLVRGDVDRSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQICDRVLVAGQRKGVIRFVGPTKFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHKLRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSAVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLIPPELAYLEKKILRNSPM 544          
BLAST of CAP-Gly domain-containing linker protein vs. Planmine SMEST
Match: SMESG000015470.1 (SMESG000015470.1)

HSP 1 Score: 929.858 bits (2402), Expect = 0.000e+0
Identity = 468/472 (99.15%), Postives = 470/472 (99.58%), Query Frame = 3
Query:  693 DCLPHVSELPSNMTTIRNNLGTLLCPTSPPGHTYYNANHRKPPETGVLSPPIRRQTPGPFPPSHLDLVSISDENEIYEAPTPVSRPVSMWRDEIDNRNLASPSRVSAKVTLQAMXXXXXXXXXXXXXXXXSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLIPPELAYLEKKILRNSPM 2108
            DCLPHVSELPSNMTTIRNNLGTLLCPTSPPGHTYYNANHRKPPETGVLSPPIRRQTPGPFPPSHLDLVSISDENEIYEAPTPVSRPVSMWRDEIDNRNLASPSRVSAKVTLQAMGLLLGDRVLVRGDVDRSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQI DRVLVAGQRKGVIRFVGPT+FSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLH LRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSS+VSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLIPPELAYLEKKILRNSPM
Sbjct:    7 DCLPHVSELPSNMTTIRNNLGTLLCPTSPPGHTYYNANHRKPPETGVLSPPIRRQTPGPFPPSHLDLVSISDENEIYEAPTPVSRPVSMWRDEIDNRNLASPSRVSAKVTLQAMGLLLGDRVLVRGDVDRSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKVDTGLNKSPLQICDRVLVAGQRKGVIRFVGPTKFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHKLRSRHKNDEIIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSAVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKGINGANLIPPELAYLEKKILRNSPM 478          
BLAST of CAP-Gly domain-containing linker protein vs. Planmine SMEST
Match: SMESG000015698.1 (SMESG000015698.1)

HSP 1 Score: 132.109 bits (331), Expect = 2.345e-31
Identity = 67/177 (37.85%), Postives = 99/177 (55.93%), Query Frame = 3
Query: 1083 SGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHG------TVDISGVAAKVDTGLNKSPLQIGDRVLVAG------QRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFA 1577
            +GI+ Y G  +F+DG+W GV LD+P GK++G V GV YFQC+E++G+F     +      ++ +P      N  N      D+        +DI            +S   +GD+V V G      Q+ G +RF+G T+F+ G W GIELD P+G+NDG+V+GVRYF C +  G+FA
Sbjct:   45 TGIVTYNGVTKFSDGIWIGVALDQPLGKHNGIVQGVTYFQCDEKHGIFVRKNNLR----HIKDKPEFEIQLNNSNLLKNSDDYPFFVPDPIMDI------------RSNFFLGDKVCVIGKSSSSTQKIGTLRFIGNTKFAEGIWVGIELDNPIGRNDGSVDGVRYFSCKSQYGLFA 205          

HSP 2 Score: 102.064 bits (253), Expect = 6.723e-22
Identity = 63/218 (28.90%), Postives = 102/218 (46.79%), Query Frame = 3
Query: 1398 IGDRVLVAG--QRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDE---IIKATQQNRLSNHSSMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSGRVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRISVKG 2036
            +GD+V +A   ++ G++ + G T+FS G W G+ LD+P+GK++G V GV YFQC    G+F   + L     RH  D+    I+    N L N                 S ++      P+M            S  + GD    +    G       ++G +R++G   F++GIWVG+EL +  G++DG V   RYF C   +G+    +++   G
Sbjct:   32 VGDKVYIASNPEKTGIVTYNGVTKFSDGIWIGVALDQPLGKHNGIVQGVTYFQCDEKHGIFVRKNNL-----RHIKDKPEFEIQLNNSNLLKN-----------------SDDYPFFVPDPIMD---------IRSNFFLGDKVCVI----GKSSSSTQKIGTLRFIGNTKFAEGIWVGIELDNPIGRNDGSVDGVRYFSCKSQYGLFALENKVQKYG 214          

HSP 3 Score: 79.7221 bits (195), Expect = 4.569e-15
Identity = 34/77 (44.16%), Postives = 49/77 (63.64%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQT 1316
            G LR+ G  +FA+G+W G+ELD P G+N G+V+GV YF C+ QYG+FA   +V K G  V    R +   N ++ Q+
Sbjct:  158 GTLRFIGNTKFAEGIWVGIELDNPIGRNDGSVDGVRYFSCKSQYGLFALENKVQKYGSIV---SRNIKTNNRMDNQS 231          
BLAST of CAP-Gly domain-containing linker protein vs. Planmine SMEST
Match: SMESG000029463.1 (SMESG000029463.1)

HSP 1 Score: 82.8037 bits (203), Expect = 6.341e-16
Identity = 33/69 (47.83%), Postives = 44/69 (63.77%), Query Frame = 3
Query: 1386 SPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKNDGAVNGVRYFQCGANRGVFAPLSRL 1592
            S  +I D VL  G  KG + F+G T F+ G WYG++LD P GKNDG+V G  YF+C  N G+F   ++L
Sbjct:    2 SDFKINDIVLYNGTLKGTVEFIGTTHFASGVWYGVKLDSPKGKNDGSVAGKSYFKCDKNHGIFVRNTQL 70          

HSP 2 Score: 68.5514 bits (166), Expect = 1.629e-11
Identity = 31/83 (37.35%), Postives = 48/83 (57.83%), Query Frame = 3
Query: 1086 GILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQYGVFAPVTRVSKV------GIEVEKRPRCVTPRNPLNPQT 1316
            G + + G   FA GVW GV+LD P+GKN G+V G  YF+C++ +G+F   T+++KV          E + R    R P++ +T
Sbjct:   18 GTVEFIGTTHFASGVWYGVKLDSPKGKNDGSVAGKSYFKCDKNHGIFVRNTQLAKVDGDESTNTSAESKVRPSALRKPMSIKT 100          

HSP 3 Score: 58.9214 bits (141), Expect = 1.251e-8
Identity = 29/71 (40.85%), Postives = 42/71 (59.15%), Query Frame = 3
Query: 1845 VLCNNEL-GIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLNKRYFLCPPDHGIMVRPSRIS-VKGINGAN 2051
            VL N  L G + ++G  +F+ G+W GV+L    GK+DG V  K YF C  +HGI VR ++++ V G    N
Sbjct:   10 VLYNGTLKGTVEFIGTTHFASGVWYGVKLDSPKGKNDGSVAGKSYFKCDKNHGIFVRNTQLAKVDGDESTN 80          
The following BLAST results are available for this feature:
BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CLIP43.574e-5142.75CAP-Gly domain containing linker protein family me... [more]
CLIP43.574e-5142.75CAP-Gly domain containing linker protein family me... [more]
CLIP31.885e-4346.77CAP-Gly domain containing linker protein 3 [Source... [more]
CLIP31.885e-4346.77CAP-Gly domain containing linker protein 3 [Source... [more]
CLIP44.409e-4328.17CAP-Gly domain containing linker protein family me... [more]
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BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tbcb-11.415e-1147.69Tubulin-specific chaperone B [Source:UniProtKB/Sw... [more]
dnc-11.478e-1033.88DyNactin Complex component [Source:UniProtKB/TrEM... [more]
dnc-11.484e-1033.88DyNactin Complex component [Source:UniProtKB/TrEM... [more]
dnc-11.563e-1033.88DyNactin Complex component [Source:UniProtKB/TrEM... [more]
dnc-11.570e-1033.88DyNactin Complex component [Source:UniProtKB/TrEM... [more]
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BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CLIP-1908.065e-3633.78gene:FBgn0020503 transcript:FBtr0332455[more]
CLIP-1901.037e-3538.89gene:FBgn0020503 transcript:FBtr0332452[more]
CLIP-1901.939e-3538.67gene:FBgn0020503 transcript:FBtr0290263[more]
CLIP-1901.952e-3538.67gene:FBgn0020503 transcript:FBtr0339796[more]
CLIP-1903.604e-3538.89gene:FBgn0020503 transcript:FBtr0290262[more]
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BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
si:dkeyp-47f9.41.071e-4641.63si:dkeyp-47f9.4 [Source:ZFIN;Acc:ZDB-GENE-131126-9... [more]
clip31.686e-4541.04CAP-GLY domain containing linker protein 3 [Source... [more]
CABZ01072096.11.128e-3839.89pep chromosome:GRCz11:10:44408675:44411032:-1 gene... [more]
clip1a3.076e-3838.34CAP-GLY domain containing linker protein 1a [Sourc... [more]
zgc:1037556.799e-3836.40zgc:103755 [Source:ZFIN;Acc:ZDB-GENE-041010-101][more]
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BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
antxr11.665e-4449.17ANTXR cell adhesion molecule 1 [Source:Xenbase;Acc... [more]
antxr11.871e-4449.17ANTXR cell adhesion molecule 1 [Source:Xenbase;Acc... [more]
antxr13.147e-4449.17ANTXR cell adhesion molecule 1 [Source:Xenbase;Acc... [more]
antxr14.776e-4449.72ANTXR cell adhesion molecule 1 [Source:Xenbase;Acc... [more]
CLIP41.904e-4239.91CAP-GLY domain containing linker protein family, m... [more]
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BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Clip42.602e-5042.86CAP-GLY domain containing linker protein family, m... [more]
Clip31.089e-4348.89CAP-GLY domain containing linker protein 3 [Source... [more]
Clip43.472e-4139.41CAP-GLY domain containing linker protein family, m... [more]
Clip46.131e-4139.41CAP-GLY domain containing linker protein family, m... [more]
Clip48.692e-4139.41CAP-GLY domain containing linker protein family, m... [more]
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BLAST of CAP-Gly domain-containing linker protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q8N3C7|CLIP4_HUMAN1.716e-5042.75CAP-Gly domain-containing linker protein 4 OS=Homo... [more]
sp|Q8CI96|CLIP4_MOUSE1.820e-4942.86CAP-Gly domain-containing linker protein 4 OS=Mus ... [more]
sp|Q5U243|CLIP3_XENLA1.813e-4449.17CAP-Gly domain-containing linker protein 3 OS=Xeno... [more]
sp|Q5R686|CLIP3_PONAB5.497e-4347.22CAP-Gly domain-containing linker protein 3 OS=Pong... [more]
sp|B9EHT4|CLIP3_MOUSE7.617e-4348.89CAP-Gly domain-containing linker protein 3 OS=Mus ... [more]
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BLAST of CAP-Gly domain-containing linker protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0B7AJW11.626e-15641.44Uncharacterized protein OS=Arion vulgaris OX=10286... [more]
A0A504Y6Y03.470e-15336.48CAP-Gly domain-containing linker protein 4 OS=Fasc... [more]
H2KRA05.072e-15139.05Phosphatidylinositol glycan class U OS=Clonorchis ... [more]
G4LY961.181e-14436.80Restin-like OS=Schistosoma mansoni OX=6183 GN=Smp_... [more]
T1KTP72.452e-13738.66Uncharacterized protein OS=Tetranychus urticae OX=... [more]
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BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
clip33.615e-4239.23CAP-Gly domain containing linker protein 3 [Source... [more]
si:dkeyp-47f9.42.418e-3735.96si:dkeyp-47f9.4 [Source:ZFIN;Acc:ZDB-GENE-131126-9... [more]
ENSAMXT00000039156.15.366e-3641.57pep primary_assembly:Astyanax_mexicanus-2.0:20:174... [more]
clip1a8.935e-3336.79CAP-Gly domain-containing linker protein 1-like [S... [more]
clip1a9.048e-3336.79CAP-Gly domain-containing linker protein 1-like [S... [more]
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BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
si:dkeyp-47f9.46.665e-4643.75si:dkeyp-47f9.4 [Source:ZFIN;Acc:ZDB-GENE-131126-9... [more]
ENSPMAT00000006020.17.361e-3032.07pep scaffold:Pmarinus_7.0:GL478150:21952:49796:1 g... [more]
ENSPMAT00000006027.11.163e-2933.02pep scaffold:Pmarinus_7.0:GL478150:21952:49796:1 g... [more]
ENSPMAT00000004647.13.002e-2631.76pep scaffold:Pmarinus_7.0:GL484285:5077:12891:1 ge... [more]
dctn1a1.049e-1344.44dynactin 1a [Source:ZFIN;Acc:ZDB-GENE-070117-2205][more]
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BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
NIP1005.132e-1751.43Large subunit of the dynactin complex; dynactin is... [more]
BIK18.920e-630.48Microtubule-associated protein; component of the i... [more]
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BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO433432.944e-5029.80Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO336002.083e-3740.83Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO302181.696e-1657.89Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO444331.298e-1450.70Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO488901.076e-1248.39Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of CAP-Gly domain-containing linker protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
si:dkeyp-47f9.42.090e-5732.08CAP-Gly domain containing linker protein family me... [more]
clip34.160e-4543.33CAP-Gly domain containing linker protein 3 [Source... [more]
clip36.829e-4443.33CAP-Gly domain containing linker protein 3 [Source... [more]
clip31.080e-4343.13CAP-Gly domain containing linker protein 3 [Source... [more]
ENSORLT00000001833.25.972e-3742.05pep primary_assembly:ASM223467v1:12:2504412:250913... [more]
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BLAST of CAP-Gly domain-containing linker protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000015465.10.000e+099.40SMESG000015465.1[more]
SMESG000015470.10.000e+099.26SMESG000015470.1[more]
SMESG000015470.10.000e+099.15SMESG000015470.1[more]
SMESG000015698.12.345e-3137.85SMESG000015698.1[more]
SMESG000029463.16.341e-1647.83SMESG000029463.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015988 ID=SMED30015988|Name=CAP-Gly domain-containing linker protein|organism=Schmidtea mediterranea sexual|type=transcript|length=2241bp
GTTTTATATAAAATTGAATGCCTTCGTTTAAATTTAAAAGGAAAATGAAA
ATGACATCACAAGATGTCTTAGTGATGAGTGTGGATACGATTTCTGAACG
ACGGAAATATCCAGAAACTCATCCGGCTGTTGAAGCACCTCTCTGTGATA
ATTGTGCTGAATATGGTCTGTGTTTTTTCGATTTGGATTGCGAAGGTTGT
ATGAAGGTTTTATTGAGTAATAGGGTAACAATAGGTCAATTGTTCGCTGT
TTTACGGCAATGGACAAAGGCTGTGCAGATTCAGCTGGAAACAATAATAG
AGCAAATGTTCAAATTAGGTGCTAATGTTAATGATAGAGATGGCTTAACT
GACATGACAATGTTACATTATGCTGCTAAATCAGGAGCTCAAGGAGAAGA
GGAATTAGCAGTGAGAGTTATAGCTTATTTGTTGGATGAAGGCGCGCTTT
TAGAAGCTCGATGCAAATGGACTGATATGACGCCATTACATTATGCTGTT
TATTTCGATGTTGCTCCAGCTGTAGAACTTCTGTTAGATCGCGGAGCAGA
CATAAGAGCCATTTGTAGAGAATTATGTAGAGGAAATTGCCTTCATATTG
CTGCCACTCAACTAGCATTGGATACAGCGAGAGTTTTAATTAAACGAGGA
GCTGATAGATATTTTCTTGATGATCGTCAGAGGACTCCTTATGACTGTTT
ACCTCATGTTTCTGAATTACCAAGTAACATGACAACGATTCGCAATAATT
TGGGAACATTACTTTGTCCAACATCTCCCCCGGGTCATACGTATTACAAT
GCTAATCATCGGAAGCCACCTGAAACGGGTGTTCTTTCCCCGCCAATTAG
AAGACAAACACCAGGTCCATTTCCTCCATCGCATTTAGACCTGGTTTCTA
TTTCAGATGAAAATGAAATATACGAGGCTCCAACTCCAGTTTCTAGACCT
GTTAGTATGTGGAGAGATGAAATAGATAATAGAAATCTTGCTAGTCCTAG
TCGCGTTTCTGCTAAAGTTACTTTACAAGCTATGGGTCTATTGCTTGGTG
ACAGGGTATTAGTTAGAGGTGATGTAGATAGGTCTGGAATCTTAAGATAT
TGCGGTCCTGTGGAATTTGCAGATGGTGTATGGGCCGGAGTTGAACTTGA
TGAACCGCAAGGCAAAAATAGTGGTACTGTCAATGGAGTATGGTATTTTC
AATGCGAAGAACAATATGGAGTATTTGCTCCAGTTACTAGAGTCAGTAAA
GTGGGAATTGAAGTTGAAAAGCGACCTCGTTGTGTTACACCAAGAAATCC
ATTAAATCCTCAAACAGTTGTCGTTGATCATGGAACCGTCGATATTTCTG
GAGTAGCGGCAAAAGTTGATACAGGTTTAAACAAATCACCCTTGCAAATA
GGTGATCGTGTATTAGTTGCTGGCCAGCGAAAAGGAGTAATTCGTTTTGT
TGGACCGACTGAATTTTCACCTGGAACATGGTACGGTATTGAATTAGACA
AACCAGTAGGTAAAAATGATGGAGCAGTTAATGGAGTACGTTATTTTCAA
TGTGGAGCCAATCGTGGTGTTTTTGCACCACTTTCTAGATTGCATATACT
TAGAAGTAGGCATAAAAATGATGAAATTATAAAAGCCACACAACAAAACA
GGTTGAGTAATCATTCCTCAATGATGACTGCTTCACAAACTGATCAGTTG
TCTAATGCTTCCTCTCCAAATCACCTTTCCATGGCTCAATCACCTCTTAT
GACATCATCATCCGTATCAACTACTCATCAATTCAATTCTCCTACATATT
TTGGTGATTCCTCCGGTCGTGTGTTTTTGAGGGAAGGTCTTCAAGTTCTT
TGCAACAATGAATTAGGTATTATTCGATATGTTGGAGCAGTTAATTTTTC
TGATGGAATTTGGGTAGGAGTTGAGTTACGTCACAAAACTGGTAAACATG
ATGGGATAGTCTTAAATAAACGCTATTTTCTTTGTCCACCCGATCATGGC
ATTATGGTGAGACCAAGTCGCATTAGCGTGAAGGGAATCAATGGAGCAAA
TCTTATTCCTCCTGAATTAGCATATCTAGAAAAGAAAATTTTGCGCAATT
CTCCAATGAGTAACAAATCTTAAAATAGCATTTAGCTTGTATCTGTTTTA
TCTTGTTTGATTTTTTATGGCTTTTATTTAAAAGATTGATGCAATAAACA
AGGAATTACTATTAAATATCTATGTTAATATTGAAAGTGAT
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protein sequence of SMED30015988-orf-1

>SMED30015988-orf-1 ID=SMED30015988-orf-1|Name=SMED30015988-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=702bp
MPSFKFKRKMKMTSQDVLVMSVDTISERRKYPETHPAVEAPLCDNCAEYG
LCFFDLDCEGCMKVLLSNRVTIGQLFAVLRQWTKAVQIQLETIIEQMFKL
GANVNDRDGLTDMTMLHYAAKSGAQGEEELAVRVIAYLLDEGALLEARCK
WTDMTPLHYAVYFDVAPAVELLLDRGADIRAICRELCRGNCLHIAATQLA
LDTARVLIKRGADRYFLDDRQRTPYDCLPHVSELPSNMTTIRNNLGTLLC
PTSPPGHTYYNANHRKPPETGVLSPPIRRQTPGPFPPSHLDLVSISDENE
IYEAPTPVSRPVSMWRDEIDNRNLASPSRVSAKVTLQAMGLLLGDRVLVR
GDVDRSGILRYCGPVEFADGVWAGVELDEPQGKNSGTVNGVWYFQCEEQY
GVFAPVTRVSKVGIEVEKRPRCVTPRNPLNPQTVVVDHGTVDISGVAAKV
DTGLNKSPLQIGDRVLVAGQRKGVIRFVGPTEFSPGTWYGIELDKPVGKN
DGAVNGVRYFQCGANRGVFAPLSRLHILRSRHKNDEIIKATQQNRLSNHS
SMMTASQTDQLSNASSPNHLSMAQSPLMTSSSVSTTHQFNSPTYFGDSSG
RVFLREGLQVLCNNELGIIRYVGAVNFSDGIWVGVELRHKTGKHDGIVLN
KRYFLCPPDHGIMVRPSRISVKGINGANLIPPELAYLEKKILRNSPMSNK
S*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
GO:0042765GPI-anchor transamidase complex
Vocabulary: biological process
TermDefinition
GO:0016255attachment of GPI anchor to protein
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
Vocabulary: INTERPRO
TermDefinition
IPR000938CAP-Gly_domain
IPR002110Ankyrin_rpt
IPR020683Ankyrin_rpt-contain_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0005509calcium ion binding