SMED30015948

Overview


Name	SMED30015948
Smed ID	SMED30015948
Length (bp)	1068

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30015948 (SMED30015948) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30015948 (SMED30015948) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30015948 (SMED30015948) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30015948 (SMED30015948) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30015948

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
pharynx	SMED30015948	SMESG000042166.1	dd_Smed_v4_4173_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
protonephridia	SMED30015948	SMESG000042166.1	dd_Smed_v4_4173_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30015948	SMESG000042166.1	dd_Smed_v4_4173_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30015948	SMESG000042166.1	dd_Smed_v4_4173_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
head region	SMED30015948	SMESG000042166.1	dd_Smed_v6_4173_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
epidermis	SMED30015948	SMESG000042166.1	dd_Smed_v4_4173_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Alignments

SMED30015948 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.001565:40768..54500 -	5062

Homology

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BLAST of SMED30015948 vs. Ensembl Celegans
Match: sli-1 (Suppressor of LIneage defect [Source:UniProtKB/TrEMBL;Acc:Q95ZR5])

HSP 1 Score: 52.7582 bits (125), Expect = 2.179e-7
Identity = 63/262 (24.05%), Postives = 114/262 (43.51%), Query Frame = 2

Query:  116 DHKFQKIFID--INNC--LYEIFATDEHKQNPIYNMLHFFEDKIDELMKLINVPDEFTNFCIQFAFIHYDLKKLFPEGSF-NKLYKLDEIAAENWWKEHFNNDLFVKAEEFGAKISKRFSES---PDIHCLLQSISFTKFDWYGNINKCSFNNFLKYYGGWENFPRYWSQIFN-NPAHLHMVTMEYSTEKLLKVKSTLGEFRSIEKSFIICLNWRIPDGQWNLVYGDETDDITVSIFNPRELLRELENKNK---YMYPLGRD 865
            D+ + KIF++  IN C  + ++F T       IYN      D+ +E  KL       T   + F+ + +++K LFPEG +    +++ +  AE++W  HF     V    F   + K    +    +   L  +I  +  D+  N     F+ F + +  ++   + W  +   +P +   +T +   ++L K+    G       S+I  L+   P GQW + Y      I  +I   + L++ L   +K   Y+YP GRD
Sbjct:  104 DNDYLKIFLESMINKCKEIIKLFKTSA-----IYN------DQSEERRKL-------TKMSLTFSHMLFEIKALFPEGIYIEDRFRMTKKEAESFWSHHFTKKNIVPWSTFFTALEKHHGSTIGKMEAAELKATIDLSGDDFISNFE---FDVFTRLFYPFKTLIKNWQTLTTAHPGYCAFLTYDEVKKRLEKLTKKPG-------SYIFRLSCTRP-GQWAIGYVAPDGKIYQTIPQNKSLIQALHEGHKEGFYIYPNGRD 336

[Close]

BLAST of SMED30015948 vs. Ensembl Celegans
Match: sli-1 (Suppressor of LIneage defect [Source:UniProtKB/TrEMBL;Acc:Q95ZR5])

HSP 1 Score: 52.373 bits (124), Expect = 2.655e-7
Identity = 63/262 (24.05%), Postives = 114/262 (43.51%), Query Frame = 2

Query:  116 DHKFQKIFID--INNC--LYEIFATDEHKQNPIYNMLHFFEDKIDELMKLINVPDEFTNFCIQFAFIHYDLKKLFPEGSF-NKLYKLDEIAAENWWKEHFNNDLFVKAEEFGAKISKRFSES---PDIHCLLQSISFTKFDWYGNINKCSFNNFLKYYGGWENFPRYWSQIFN-NPAHLHMVTMEYSTEKLLKVKSTLGEFRSIEKSFIICLNWRIPDGQWNLVYGDETDDITVSIFNPRELLRELENKNK---YMYPLGRD 865
            D+ + KIF++  IN C  + ++F T       IYN      D+ +E  KL       T   + F+ + +++K LFPEG +    +++ +  AE++W  HF     V    F   + K    +    +   L  +I  +  D+  N     F+ F + +  ++   + W  +   +P +   +T +   ++L K+    G       S+I  L+   P GQW + Y      I  +I   + L++ L   +K   Y+YP GRD
Sbjct:  104 DNDYLKIFLESMINKCKEIIKLFKTSA-----IYN------DQSEERRKL-------TKMSLTFSHMLFEIKALFPEGIYIEDRFRMTKKEAESFWSHHFTKKNIVPWSTFFTALEKHHGSTIGKMEAAELKATIDLSGDDFISNFE---FDVFTRLFYPFKTLIKNWQTLTTAHPGYCAFLTYDEVKKRLEKLTKKPG-------SYIFRLSCTRP-GQWAIGYVAPDGKIYQTIPQNKSLIQALHEGHKEGFYIYPNGRD 336

[Close]

BLAST of SMED30015948 vs. Ensembl Celegans
Match: sli-1 (Suppressor of LIneage defect [Source:UniProtKB/TrEMBL;Acc:Q95ZR5])

HSP 1 Score: 52.373 bits (124), Expect = 2.837e-7
Identity = 63/262 (24.05%), Postives = 114/262 (43.51%), Query Frame = 2

Query:  116 DHKFQKIFID--INNC--LYEIFATDEHKQNPIYNMLHFFEDKIDELMKLINVPDEFTNFCIQFAFIHYDLKKLFPEGSF-NKLYKLDEIAAENWWKEHFNNDLFVKAEEFGAKISKRFSES---PDIHCLLQSISFTKFDWYGNINKCSFNNFLKYYGGWENFPRYWSQIFN-NPAHLHMVTMEYSTEKLLKVKSTLGEFRSIEKSFIICLNWRIPDGQWNLVYGDETDDITVSIFNPRELLRELENKNK---YMYPLGRD 865
            D+ + KIF++  IN C  + ++F T       IYN      D+ +E  KL       T   + F+ + +++K LFPEG +    +++ +  AE++W  HF     V    F   + K    +    +   L  +I  +  D+  N     F+ F + +  ++   + W  +   +P +   +T +   ++L K+    G       S+I  L+   P GQW + Y      I  +I   + L++ L   +K   Y+YP GRD
Sbjct:  121 DNDYLKIFLESMINKCKEIIKLFKTSA-----IYN------DQSEERRKL-------TKMSLTFSHMLFEIKALFPEGIYIEDRFRMTKKEAESFWSHHFTKKNIVPWSTFFTALEKHHGSTIGKMEAAELKATIDLSGDDFISNFE---FDVFTRLFYPFKTLIKNWQTLTTAHPGYCAFLTYDEVKKRLEKLTKKPG-------SYIFRLSCTRP-GQWAIGYVAPDGKIYQTIPQNKSLIQALHEGHKEGFYIYPNGRD 353

[Close]

BLAST of SMED30015948 vs. TrEMBL
Match: A0A0K0FQI5 (E3 ubiquitin-protein ligase CBL-B (inferred by orthology to a human protein) OS=Strongyloides venezuelensis OX=75913 PE=4 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 9.783e-8
Identity = 58/226 (25.66%), Postives = 111/226 (49.12%), Query Frame = 2

Query:  209 MLHFFEDKIDELMKLINVPD-EFTNFCIQFAFIHYDLKKLFPEGSFNKLYKLDEIAAENWWKEHFNNDLFVKAEEFGA--KISKRFSES-PDIHCLLQSISFTKFDWYGNINKCSFNNFLKYYGGWENFPRYWSQI-FNNPAHLHMVTMEYSTEKLLKVKSTLGEFRSIEKSFIICLNWRIPDGQWNLVYGDETDDITVSIFNPRELLRELENKNK---YMYPLGR 862
            ++  F+D  D +    ++   + T   + F+ ++Y+L  L PEG+FN  YK+ ++ AE +WK +FN+ + V  EEF    K    F E+  + +CL +++  T  ++   I+K  F+ F + +  W N    W  +   +PA+   +T +       +V+  L  + S   S++  L+     G W + Y     +I  +I + + L++ L + N+   Y YP G+
Sbjct:  119 VIRLFKDNKDLICDKTSLARLKLTKHILTFSHMYYELIALSPEGNFNFNYKMTKLDAEEFWKRNFNDKIIVSWEEFQPILKNVHHFEENCEESNCLKKTMDLTSSNY---ISKFEFDVFTRLFQPWSNLLDNWRVLALKHPAYGSFLTYD-------QVRLILQNYLSKPGSYVFRLSC-TQLGNWAIGYVAPNKNIYQTIPSNKSLIQALVDGNRDGYYCYPNGQ 333

[Close]

BLAST of SMED30015948 vs. TrEMBL
Match: A0A0N5BL95 (Uncharacterized protein OS=Strongyloides papillosus OX=174720 PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 1.442e-7
Identity = 55/205 (26.83%), Postives = 102/205 (49.76%), Query Frame = 2

Query:  269 EFTNFCIQFAFIHYDLKKLFPEGSFNKLYKLDEIAAENWWKEHFNNDLFVKAEEFGA--KISKRFSES-PDIHCLLQSISFTKFDWYGNINKCSFNNFLKYYGGWENFPRYWSQI-FNNPAHLHMVTMEYSTEKLLKVKSTLGEFRSIEKSFIICLNWRIPDGQWNLVYGDETDDITVSIFNPRELLRELENKNK---YMYPLGR 862
            + T   + F+ ++Y+L  L PEG+FN  YK+ ++ AE +WK +FN+ + V  EEF    K    F E+  + +CL +++  T  ++   I+K  F+ F + +  W N    W  +   +PA+   +T +       +V+  L  + S   S++  L+     G W + Y     +I  +I + + L++ L + N+   Y YP G+
Sbjct:  140 KLTKHILTFSHMYYELIALSPEGNFNFNYKMTKLDAEEFWKGNFNDKIIVSWEEFQPILKNVHHFEENCEESNCLKKTMDLTSSNY---ISKFEFDVFTRLFQPWSNLLDNWRVLALKHPAYGSFLTYD-------QVRLILQNYLSKPGSYVFRLSC-TQLGNWAIGYVAPNKNIYQTIPSNKSLIQALVDGNRDGYYCYPNGQ 333

[Close]

BLAST of SMED30015948 vs. TrEMBL
Match: A0A0K0E5H0 (Uncharacterized protein OS=Strongyloides stercoralis OX=6248 PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 1.472e-7
Identity = 55/227 (24.23%), Postives = 110/227 (48.46%), Query Frame = 2

Query:  209 MLHFFEDKIDELMKLINVPD-EFTNFCIQFAFIHYDLKKLFPEGSFNKLYKLDEIAAENWWKEHFNNDLFVKAEEFGAKISK--RFSE-SPDIHCLLQSISFTKFDWYGNINKCSFNNFLKYYGGWENFPRYWSQI-FNNPAHLHMVTMEYSTEKLLKVKSTLGEFRSIEKSFIICLNWRIPDGQWNLVYGDETDDITVSIFNPRELLRELENKNK---YMYPLGRD 865
            ++  F+D  D +    ++   + T   + F+ ++Y+L  L PEG+F+  YK+ ++ A+ +WKE+F +   +  E+F   + K   F E   + +CL +++  T  +W   I+K  F+ F + +  W N    W  +   +PA+ + +T +       +V+  L  + S   S++  L+     G W + Y     +I  +I   + L++ L +  +   Y YP G+D
Sbjct:  119 VIKLFKDNKDSIYDKASLARFKLTKHILTFSHMYYELIALSPEGNFDIEYKITKLDADEFWKENFKDKTIISWEDFQFTLKKIHHFEEDCEESNCLKRTMDLTSNNW---ISKFEFDVFTRLFQPWSNLLDNWRILALKHPAYANFLTYD-------QVRLVLSNYLSKPGSYVFRLSC-TQLGNWAIGYVAPNKNIYQTIPTNKSLIQALVDGYREGYYCYPNGQD 334

[Close]

BLAST of SMED30015948 vs. TrEMBL
Match: W6V060 (E3 ubiquitin-protein ligase CBL-B OS=Echinococcus granulosus OX=6210 GN=EGR_00761 PE=4 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 2.276e-7
Identity = 47/197 (23.86%), Postives = 92/197 (46.70%), Query Frame = 2

Query:  200 IYNMLHFFEDKIDELMKLINVPDEFTNFCIQFAFIHYDLKKLFPEGSFNKLYKLDEIAAENWWKEHFNNDLFVKAEEFGAKISKRFSE-SPDIHCLLQSISFTKFDWYGNINKCSFNNFLKYYGGWENFPRYWSQIFNNPAHLHMVTMEYSTEKLLKVKSTLGEFRSIEKSFIICLNWRIPDGQWNLVYGDETDDIT 787
            +  +L  FE ++    K  ++ + F+ +   F  +  ++K LFP G F          A+NWW+ +FN  + V + EF A+    F + + D   L+ ++ F+  D+   I+K   + F + +G W    R W ++ N+P +    T   + + L + ++T G +     +F I ++    +G W++ Y      IT
Sbjct:  195 LTKLLKQFESRLSTGSKNFDM-ETFSKYTFFFYHMAEEIKFLFPSGKFATNQVFGRKDAQNWWERNFNPRVAVASAEFLARFRNEFPDVNEDGDALIDTMGFSSLDY---ISKYDLDIFTRLFGAWPFVTRIWRRLVNHPGYQKYGTFNTTFDVLNRCRTTPGSY-----TFRISMSR---NGYWSIGYVSAEGKIT 379

[Close]

BLAST of SMED30015948 vs. TrEMBL
Match: A0A068YJL3 (Expressed protein OS=Echinococcus multilocularis OX=6211 GN=EmuJ_001016000 PE=4 SV=2)

HSP 1 Score: 63.5438 bits (153), Expect = 2.690e-7
Identity = 44/179 (24.58%), Postives = 86/179 (48.04%), Query Frame = 2

Query:  254 INVPDEFTNFCIQFAFIHYDLKKLFPEGSFNKLYKLDEIAAENWWKEHFNNDLFVKAEEFGAKISKRFSE-SPDIHCLLQSISFTKFDWYGNINKCSFNNFLKYYGGWENFPRYWSQIFNNPAHLHMVTMEYSTEKLLKVKSTLGEFRSIEKSFIICLNWRIPDGQWNLVYGDETDDIT 787
            + +  ++T+F   F  +  ++K LFP G F          A+NWW+ +FN+ + V + EF A+    F + + D   L+ ++ F+  D+   I+K   + F + +G W    R W ++ N+P +    T   + + L + ++T G +     +F I ++    +G W++ Y      IT
Sbjct:  109 MEIISKYTSF---FYHMAEEIKFLFPSGKFATNQVFGRKDAQNWWERNFNSRVAVASAEFLARFRNEFPDVNEDGDALIDTMGFSSLDY---ISKYDLDLFTRLFGAWPFVTRIWRRLVNHPGYQKYGTFNTTFDVLNRCRTTPGSY-----TFRISMSR---NGYWSIGYVSAEGKIT 273

[Close]

BLAST of SMED30015948 vs. Ensembl Cavefish
Match: cblc (E3 ubiquitin-protein ligase CBL-like [Source:NCBI gene;Acc:103042889])

HSP 1 Score: 55.4546 bits (132), Expect = 5.062e-8
Identity = 50/207 (24.15%), Postives = 96/207 (46.38%), Query Frame = 2

Query:  272 FTNFCIQFAFIHYDLKKLFPEGSF-NKLYKLDEIAAENWWKEHFNNDLFVKAEEFGAKISK--RFSESPDIHCLLQSISFTKFDWYGNINKCSFNNFLKYYGGWENFPRYWSQI-FNNPAHLHMVTMEYSTEKLLKVKSTLGEFRSIEKSFIICLNW-RIPDGQWNLVYGDETDDITVSIFNPRELLRELEN---KNKYMYPLGRDL 868
             T   + F+ + ++L+ +FP+G F   +YKL +  A+ +WK+ F     V+  +F  ++ +   F E  +   L  ++  T  D   +I+   F+ F + +  W    R W+Q+   +P ++  +T +       +V++ L   R    S+I  L+  R+  GQW + +      I  +I     L + L     +  Y+YP GRD+
Sbjct:  132 LTKLSLLFSHMLWELRAMFPDGRFQGDIYKLTKTEAQEFWKKTFGYRCIVQWSQFKHQLKRVHGFEEGMEAMALKSTVDLTCND---HISVFEFDIFTRLFQPWSTLLRNWNQLAVTHPGYMAFLTYD-------QVRARLEHHRHRPGSYIFRLSCTRM--GQWAVGHVTFDGSIVQTIPENTPLFQALIQGFREGCYLYPDGRDV 326

[Close]

Query:  272 FTNFCIQFAFIHYDLKKLFPEGSF-NKLYKLDEIAAENWWKEHFNNDLFVKAEEFGAKISK--RFSESPDIHCLLQSISFTKFDWYGNINKCSFNNFLKYYGGWENFPRYWSQI-FNNPAHLHMVTMEYSTEKLLKVKSTLGEFRSIEKSFIICLNW-RIPDGQWNLVYGDETDDITVSIFNPRELLRELEN---KNKYMYPLGRDL 868
             T   + F+ + ++L+ +FP+G F   +YKL +  A+ +WK+ F     V+  +F  ++ +   F E  +   L  ++  T  D   +I+   F+ F + +  W    R W+Q+   +P ++  +T +       +V++ L   R    S+I  L+  R+  GQW + +      I  +I     L + L     +  Y+YP GRD+
Sbjct:  132 LTKLSLLFSHMLWELRAMFPDGRFQGDIYKLTKTEAQEFWKKTFGYRCIVQWSQFKHQLKRVHGFEEGMEAMALKSTVDLTCND---HISVFEFDIFTRLFQPWSTLLRNWNQLAVTHPGYMAFLTYD-------QVRARLEHHRHRPGSYIFRLSCTRM--GQWAVGHVTFDGSIVQTIPENTPLFQALIQGFREGCYLYPDGRDV 326

[Close]

BLAST of SMED30015948 vs. Ensembl Medaka
Match: cblc (E3 ubiquitin-protein ligase CBL [Source:NCBI gene;Acc:101158476])

HSP 1 Score: 53.1434 bits (126), Expect = 2.260e-7
Identity = 53/207 (25.60%), Postives = 90/207 (43.48%), Query Frame = 2

Query:  272 FTNFCIQFAFIHYDLKKLFPEGSF-NKLYKLDEIAAENWWKEHFNNDLFVKAEEFGAKISK--RFSESPDIHCLLQSISFTKFDWYGNINKCSFNNFLKYYGGWENFPRYWSQI-FNNPAHLHMVTMEYSTEKLLKVKSTLGEFRSIEKSFIICLNW-RIPDGQWNLVYGDETDDITVSIFNPRELLRELENKNK---YMYPLGRDL 868
             T   + F+ + ++LK +FP G F    YKL +  AE +W+  F N   V    F  ++     F E  +   L  +I  T  D+   I+   F+ F + +  W +  R W+ +   +P ++  +T +   E+L       G       S+I  L+  R+  GQW + +     DI  ++     L + L    K   Y+YP GRD+
Sbjct:  129 LTKLSLLFSHMLWELKAMFPGGHFQGDTYKLTKSKAEEFWRRSFGNKCIVPWNHFKQQLRTVHPFEERMEAMALKSTIDLTCNDY---ISVFEFDIFTRLFQPWSSLIRNWNHMAVTHPGYMAFLTYDQVVERLEHYLHKPG-------SYIFRLSCTRM--GQWAVGHVTAEGDIIQTLPGDTPLYQALIQGFKEGCYLYPDGRDV 323

[Close]

Query:  272 FTNFCIQFAFIHYDLKKLFPEGSF-NKLYKLDEIAAENWWKEHFNNDLFVKAEEFGAKISK--RFSESPDIHCLLQSISFTKFDWYGNINKCSFNNFLKYYGGWENFPRYWSQI-FNNPAHLHMVTMEYSTEKLLKVKSTLGEFRSIEKSFIICLNW-RIPDGQWNLVYGDETDDITVSIFNPRELLRELENKNK---YMYPLGRDL 868
             T   + F+ + ++LK +FP G F    YKL +  AE +W+  F N   V    F  ++     F E  +   L  +I  T  D+   I+   F+ F + +  W +  R W+ +   +P ++  +T +   E+L       G       S+I  L+  R+  GQW + +     DI  ++     L + L    K   Y+YP GRD+
Sbjct:  129 LTKLSLLFSHMLWELKAMFPGGHFQGDTYKLTKSKAEEFWRRSFGNKCIVPWNHFKQQLRTVHPFEERMEAMALKSTIDLTCNDY---ISVFEFDIFTRLFQPWSSLIRNWNHMAVTHPGYMAFLTYDQVVERLEHYLHKPG-------SYIFRLSCTRM--GQWAVGHVTAEGDIIQTLPGDTPLYQALIQGFKEGCYLYPDGRDV 323

[Close]

BLAST of SMED30015948 vs. Planmine SMEST
Match: SMESG000042166.1 (SMESG000042166.1)

HSP 1 Score: 471.085 bits (1211), Expect = 6.757e-169
Identity = 228/236 (96.61%), Postives = 231/236 (97.88%), Query Frame = 2

Query:  209 MLHFFEDKIDELMKLINVPDEFTNFCIQFAFIHYDLKKLFPEGSFNKLYKLDEIAAENWWKEHFNNDLFVKAEEFGAKISKRFSESPDIHCLLQSISFTKFDWYGNINKCSFNNFLKYYGGWENFPRYWSQIFNNPAHLHMVTMEYSTEKLLKVKSTLGEFRSIEKSFIICLNWRIPDGQWNLVYGDETDDITVSIFNPRELLRELENKNKYMYPLGRDLKRNSIRYLIPFKMMKI 916
            MLHFFEDKIDELMKLINVPDEFTNFCIQFAFIHYDLKKLFPEGSFNKLYKLDEIAAENWWKEHFNNDLFVKAEEFGAKISKRFSESPDIHCLLQSISFTKFDWYGNINKCSFNNFLKYYGGWENFPRYWSQIFNNPAHLHMVTMEYSTEKLLKVKSTLGEFRSIEKSFIICLNWRIPDGQWNLVYGDETDDITVSIFNPRELLRELENKNKYMYPLGRDLKRNSI+ L P  + +I
Sbjct:    1 MLHFFEDKIDELMKLINVPDEFTNFCIQFAFIHYDLKKLFPEGSFNKLYKLDEIAAENWWKEHFNNDLFVKAEEFGAKISKRFSESPDIHCLLQSISFTKFDWYGNINKCSFNNFLKYYGGWENFPRYWSQIFNNPAHLHMVTMEYSTEKLLKVKSTLGEFRSIEKSFIICLNWRIPDGQWNLVYGDETDDITVSIFNPRELLRELENKNKYMYPLGRDLKRNSIQ-LRPISLPEI 235

[Close]

BLAST of SMED30015948 vs. Planmine SMEST
Match: SMESG000042167.1 (SMESG000042167.1)

HSP 1 Score: 155.992 bits (393), Expect = 6.608e-46
Identity = 75/195 (38.46%), Postives = 118/195 (60.51%), Query Frame = 2

Query:  278 NFCIQFAFIHYDLKKLFPEGSFNKLYKLDEIAAENWWKEHFNNDLFVKAEEFGAKISKRFSESPDIHCLLQSISFTKFDWYGNINKCSFNNFLKYYGGWENFPRYWSQIFNNPAHLHMVTMEYSTEKLLKVKSTLG-EFRSIEKSFIICLNWRIPDGQWNLVYGDETDDITVSIFNPRELLRELENKNKYMYPLG 859
            N CI F F++ DL+ LFP G F K Y L+E +AE WWK +FNN++F++ E F    S +F     I+ +++ I FT  +W G INK SFN FL+YYGGW     +W  I  +P +++++T+E   E+  K++  +    ++IEKS++  ++W  PDG W L+YGD+  +I   I    +L+  L+   K+ +P G
Sbjct:    2 NMCIHFKFLNRDLETLFPNGVFVKKYFLEEDSAEEWWKNNFNNEIFIEPERFFIPFSDKFPTLSRINDVMKRICFTSLEWCGYINKNSFNMFLRYYGGWYKIDSFWLDITTHPGNMNLITLESGLEEFEKIQRMMAFSNKNIEKSYLFFIDWTSPDGLWILIYGDKNVEIKYEILKIEQLMSSLKTSTKFQFPRG 196

[Close]

BLAST of SMED30015948 vs. Planmine SMEST
Match: SMESG000042167.1 (SMESG000042167.1)

HSP 1 Score: 154.451 bits (389), Expect = 2.898e-45
Identity = 74/193 (38.34%), Postives = 117/193 (60.62%), Query Frame = 2

Query:  284 CIQFAFIHYDLKKLFPEGSFNKLYKLDEIAAENWWKEHFNNDLFVKAEEFGAKISKRFSESPDIHCLLQSISFTKFDWYGNINKCSFNNFLKYYGGWENFPRYWSQIFNNPAHLHMVTMEYSTEKLLKVKSTLG-EFRSIEKSFIICLNWRIPDGQWNLVYGDETDDITVSIFNPRELLRELENKNKYMYPLG 859
            CI F F++ DL+ LFP G F K Y L+E +AE WWK +FNN++F++ E F    S +F     I+ +++ I FT  +W G INK SFN FL+YYGGW     +W  I  +P +++++T+E   E+  K++  +    ++IEKS++  ++W  PDG W L+YGD+  +I   I    +L+  L+   K+ +P G
Sbjct:    2 CIHFKFLNRDLETLFPNGVFVKKYFLEEDSAEEWWKNNFNNEIFIEPERFFIPFSDKFPTLSRINDVMKRICFTSLEWCGYINKNSFNMFLRYYGGWYKIDSFWLDITTHPGNMNLITLESGLEEFEKIQRMMAFSNKNIEKSYLFFIDWTSPDGLWILIYGDKNVEIKYEILKIEQLMSSLKTSTKFQFPRG 194

[Close]

BLAST of SMED30015948 vs. Planmine SMEST
Match: SMESG000006795.1 (SMESG000006795.1)

HSP 1 Score: 51.2174 bits (121), Expect = 7.718e-7
Identity = 50/232 (21.55%), Postives = 94/232 (40.52%), Query Frame = 2

Query:  191 QNPIYNMLHFFEDKIDELMKLINVPDE-----FTNFCIQFAFIHYDLKKLFPEGSFNKL-YKLDEIAAENWWKEHFNNDLFVKAEEFGAKISKRFSESPDIHCLLQSISFTKFDWYGNINKCSFNNFLKYYGGWENFPRYWSQIFNNPAHLHMVTMEYSTEKLLKVKSTLGEFRSIEKSFIICLNWRIPDGQWNLVY---GDETDDITVSIFNPRELLRELENKNKYMYPLG 859
            +N + N+ + F   + +   LI   D+     F    + F F++++++  FP G ++    K+ +  A  WW++ F +++FV  +     +SK                             +F+ F + Y  WE     W  I+++PA+LH  T           K TL  + +   +++  ++ +   G W + Y     E   + V   N  E L E   +N Y+YP G
Sbjct:   64 KNNMINLYNSFAKFVQDNGPLIGTKDDHMRKTFFTKVVHFQFLYFEIQAYFPNGIYSPHNVKISKNPARMWWEKKFGDNIFVDLDVRKYSLSK-----------------------------TFDLFTRLYSPWERILEVWRVIYSHPAYLHFSTYG-------DTKDTLSRYLNEPGTYVFRISCKDL-GFWTIGYVKSSKEMGQVKVDKSNLIEALVEGNKRNMYLYPNG 258

The following BLAST results are available for this feature:

BLAST of SMED30015948 vs. Ensembl Human
BLAST of SMED30015948 vs. Ensembl Celegans
BLAST of SMED30015948 vs. Ensembl Fly
BLAST of SMED30015948 vs. Ensembl Zebrafish
BLAST of SMED30015948 vs. Ensembl Xenopus
BLAST of SMED30015948 vs. Ensembl Mouse
BLAST of SMED30015948 vs. UniProt/SwissProt
BLAST of SMED30015948 vs. TrEMBL
BLAST of SMED30015948 vs. Ensembl Cavefish
BLAST of SMED30015948 vs. Ensembl Sea Lamprey
BLAST of SMED30015948 vs. Ensembl Yeast
BLAST of SMED30015948 vs. Ensembl Nematostella
BLAST of SMED30015948 vs. Ensembl Medaka
BLAST of SMED30015948 vs. Planmine SMEST

BLAST of SMED30015948 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30015948 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3


Match Name	E-value	Identity	Description
sli-1	2.179e-7	24.05	Suppressor of LIneage defect [Source:UniProtKB/Tr...	[more]
sli-1	2.655e-7	24.05	Suppressor of LIneage defect [Source:UniProtKB/Tr...	[more]
sli-1	2.837e-7	24.05	Suppressor of LIneage defect [Source:UniProtKB/Tr...	[more]

BLAST of SMED30015948 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30015948 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30015948 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30015948 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30015948 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30015948 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K0FQI5	9.783e-8	25.66	E3 ubiquitin-protein ligase CBL-B (inferred by ort...	[more]
A0A0N5BL95	1.442e-7	26.83	Uncharacterized protein OS=Strongyloides papillosu...	[more]
A0A0K0E5H0	1.472e-7	24.23	Uncharacterized protein OS=Strongyloides stercoral...	[more]
W6V060	2.276e-7	23.86	E3 ubiquitin-protein ligase CBL-B OS=Echinococcus ...	[more]
A0A068YJL3	2.690e-7	24.58	Expressed protein OS=Echinococcus multilocularis O...	[more]

BLAST of SMED30015948 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
cblc	5.062e-8	24.15	E3 ubiquitin-protein ligase CBL-like [Source:NCBI ...	[more]
cblc	5.062e-8	24.15	E3 ubiquitin-protein ligase CBL-like [Source:NCBI ...	[more]

BLAST of SMED30015948 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30015948 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30015948 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30015948 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2


Match Name	E-value	Identity	Description
cblc	2.260e-7	25.60	E3 ubiquitin-protein ligase CBL [Source:NCBI gene;...	[more]
cblc	2.260e-7	25.60	E3 ubiquitin-protein ligase CBL [Source:NCBI gene;...	[more]

BLAST of SMED30015948 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4


Match Name	E-value	Identity	Description
SMESG000042166.1	6.757e-169	96.61	SMESG000042166.1	[more]
SMESG000042167.1	6.608e-46	38.46	SMESG000042167.1	[more]
SMESG000042167.1	2.898e-45	38.34	SMESG000042167.1	[more]
SMESG000006795.1	7.718e-7	21.55	SMESG000006795.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30015948 ID=SMED30015948|Name=SMED30015948|organism=Schmidtea mediterranea sexual|type=transcript|length=1068bp
AAATCTATATAAATCTTATATGTCTTTGACTGAGGTGTTTACATCTGAAA
AATTTCGAAGGTTTTCCGGAATTTTAGAAAACGTCGTAACAAATATTGTT
AGAAATGACAATTTTGACCACAAATTTCAAAAGATTTTTATTGACATCAA
CAATTGTCTCTATGAAATTTTTGCCACTGATGAACACAAGCAAAATCCTA
TTTATAATATGCTACATTTTTTTGAAGACAAAATTGATGAATTGATGAAA
TTAATTAATGTTCCAGATGAATTTACAAATTTTTGTATTCAATTTGCTTT
TATCCACTATGATCTAAAGAAATTATTTCCCGAAGGATCATTTAATAAAT
TATACAAATTGGATGAAATTGCTGCTGAAAATTGGTGGAAAGAGCATTTT
AATAACGATTTATTTGTGAAAGCTGAAGAATTCGGTGCTAAAATCAGTAA
AAGATTCTCAGAATCCCCAGACATTCACTGTTTGTTACAATCTATTTCAT
TTACCAAGTTTGATTGGTATGGTAATATCAATAAATGCAGTTTTAATAAT
TTTTTAAAATATTATGGAGGGTGGGAAAATTTCCCCAGATATTGGTCACA
AATTTTTAATAATCCAGCCCATTTGCACATGGTGACAATGGAATACTCCA
CGGAGAAACTATTGAAAGTCAAATCAACTTTAGGAGAATTTAGAAGCATT
GAAAAATCTTTCATAATCTGCTTAAATTGGCGTATACCCGATGGACAATG
GAATTTAGTTTACGGTGACGAAACTGATGACATTACAGTTTCAATTTTCA
ATCCTCGCGAGCTTTTAAGAGAATTGGAAAATAAAAACAAATATATGTAT
CCTCTTGGACGTGACTTAAAAAGAAACTCAATTAGATACCTGATACCATT
CAAAATGATGAAAATTATTCAACAATTGGATCATACGAGTCTACTCAATA
ACTGTGGGGGTACAATTCGGAAACTTGCTAACAAACAGAATAAAATTATG
ACATATATATCACTTGTTTTTAATAATTTAAAATTAAAATACATTTGTTT
TTAATATTGTAAAAAAAA

protein sequence of SMED30015948-orf-1

>SMED30015948-orf-1 ID=SMED30015948-orf-1|Name=SMED30015948-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=351bp
NLYKSYMSLTEVFTSEKFRRFSGILENVVTNIVRNDNFDHKFQKIFIDIN
NCLYEIFATDEHKQNPIYNMLHFFEDKIDELMKLINVPDEFTNFCIQFAF
IHYDLKKLFPEGSFNKLYKLDEIAAENWWKEHFNNDLFVKAEEFGAKISK
RFSESPDIHCLLQSISFTKFDWYGNINKCSFNNFLKYYGGWENFPRYWSQ
IFNNPAHLHMVTMEYSTEKLLKVKSTLGEFRSIEKSFIICLNWRIPDGQW
NLVYGDETDDITVSIFNPRELLRELENKNKYMYPLGRDLKRNSIRYLIPF
KMMKIIQQLDHTSLLNNCGGTIRKLANKQNKIMTYISLVFNNLKLKYICF
*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000020	protonephridia
PLANA:0000034	epidermis
PLANA:0000418	head
PLANA:0002032	epidermal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR011992	EF-hand-dom_pair
IPR014741	Adaptor_Cbl_EF_hand-like
IPR024159	Cbl_PTB

Vocabulary: **molecular function**
Term	Definition
GO:0005509	calcium ion binding
GO:0001784	phosphotyrosine binding
GO:0004842	ubiquitin-protein transferase activity
GO:0004871	signal transducer activity
GO:0008270	zinc ion binding
GO:0016874	ligase activity

Vocabulary: **cellular component**
Term	Definition
GO:0005634	nucleus

Vocabulary: **biological process**
Term	Definition
GO:0007166	cell surface receptor signaling pathway
GO:0016567	protein ubiquitination
GO:0023051	regulation of signaling

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014741	Adaptor protein Cbl, EF hand-like	PFAM	PF02761	Cbl_N2	coord: 49..131 e-value: 9.0E-5 score: 23.1
IPR024159	Adaptor protein Cbl, PTB domain	PROSITE	PS51506	CBL_PTB	coord: 1..226 score: 12.762

Main menu

SMED30015948

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Owlery

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