Transposon Ty3-I Gag-Pol polyprotein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30015933 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Zebrafish
Match: CR925755.2 (pep chromosome:GRCz11:17:42486740:42492668:-1 gene:ENSDARG00000116402.1 transcript:ENSDART00000183946.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR925755.2) HSP 1 Score: 140.969 bits (354), Expect = 5.174e-34 Identity = 119/425 (28.00%), Postives = 187/425 (44.00%), Query Frame = 1 Query: 772 MEDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNSXXXXXXXXXXXXXXXS*DHFSD*RTTTEDSSKTM------AESQNRFMSQ----HLYVTN---------------ESFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHVKG----ICYSLWFETYDSV*KRLLYNEEKPSRCT*ICYEF*RIFLRK---KFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLA----EYDFELKYKKGEEHSNADGLSRL 1938 ME I + AK+I+ S A V KKDGS+R C+DYRGLN+IT+K ++ + + L A F+ +DL AY+ V + P + +TAF+ G F + L LS AP FQ L++ VL +M+ + + VYLDDILI+ S ++H + + + +R+ GL + EKC F+ + FLGHT + E ++ + + + T DS K + A RF+ +TN +F LK APIL P R F++E S +G++ S I +F S +R + + E R +L FI+ TDH ++ + K + + A A ++F + Y+ G ++ D LSRL Sbjct: 513 MEKYISDSLAAKIIRPSSSPAGAGFFFVKKKDGSLRPCIDYRGLNSITVKNTYPLPLMSSAFERLQGANFFTKLDLRNAYHLVRIRPGDEWKTAFNTPRGHFEYCVLPFGLSNAPAVFQALVNDVLRDMIDQFIYVYLDDILIFSHSLQEHVQHVRRVLQRLLENGLYVKAEKCVFHAQSVQFLGHTVSVEGMRMDPEKIQAVV----------------NWPTPDSRKALQRFLGFANFYRRFIRNFRQLAAPLTNLTSSKTPFRWSNAAEAAFSKLKGCFVSAPILIAPDPSRQFVVEVDVSEVGVGAILSQRSALDGKIHPCAYFSHRLSAAERNYDIGNRELLAVKLALEEWRHWLEGSGVPFIVSTDHKNLEYIKSAK-----RLNSRQARWALFFGRFNFSISYRPGSKNIKPDALSRL 916
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Zebrafish
Match: BX511082.1 (pep chromosome:GRCz11:9:14291932:14297132:1 gene:ENSDARG00000113678.1 transcript:ENSDART00000183119.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX511082.1) HSP 1 Score: 139.043 bits (349), Expect = 1.991e-33 Identity = 121/433 (27.94%), Postives = 186/433 (42.96%), Query Frame = 1 Query: 772 MEDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNSXXXXXXXXXXXXXXXS*DHFSD*RTTTEDSSKTM------AESQNRFMSQHLYVTNE-------------------SFIVLKNRLCVAPILDYPREDRTFILETYAS---FGIIGSVWSHVKGICYSLWF---------ETYDSV*KRLLYNEEKPSRCT*ICYEF*RIFLRK---KFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLA----EYDFELKYKKGEEHSNADGLSRL 1938 ME I + AK+I+ S A V KKDGS+R C+DYRGLNAIT+K ++ + + L A F+ +DL AY+ V + + +TAF+ G F + L LS AP FQ L++ VL +ML + + VYLDDILI+ S ++H + + + +R+ GL + EKC F+ + FLGH + E ++ + + + T DS K + A RF+ + +F LK+ APIL P R F++E AS G I S S G + + + YD + LL + E R +L FI+ TDH ++ + K + + A A +DF + Y+ G ++ D LSR+ Sbjct: 540 MEKYISDSLAAKIIRPSSSPAGAGFFFVKKKDGSLRPCIDYRGLNAITVKNTYPLPLMSSAFERLQGASFFTKLDLRNAYHLVRIREGDEWKTAFNTPRGHFEYCVLPFGLSNAPAVFQALVNDVLRDMLDQFIYVYLDDILIFSHSLQEHVQHVRRVLQRLLENGLYVKAEKCVFHAQSVPFLGHIVSVEGMRMDPEKVQAVVDW----------------PTPDSRKALQRFLGFANFYRRFIRNFSQLAAPLTALTSLKTPFRWSNAAQVAFDRLKSCFVSAPILIAPDPSRQFVVEVDASEVGVGAILSQRSSSDGKMHPCAYFSHRLNNAEQNYDIGNRELL--------AVKLALEEWRHWLEGSGVPFIVWTDHKNLEYIQSAK-----RLNSRQARWALFFGRFDFSISYRPGSKNVKPDALSRI 943
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Zebrafish
Match: BX511224.1 (pep chromosome:GRCz11:2:18017000:18022765:1 gene:ENSDARG00000113243.1 transcript:ENSDART00000186877.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX511224.1) HSP 1 Score: 138.272 bits (347), Expect = 3.865e-33 Identity = 119/423 (28.13%), Postives = 190/423 (44.92%), Query Frame = 1 Query: 772 MEDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNSXXXXXXXXXXXXXXXS---------------*DHFSD*RTTTEDSSKTMAESQNRFMSQHLYVTNESFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSH-------VKGICY-----SLWFETYDSV*KRLLYNEEKPSRCT*ICYEF*RIFLRK---KFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLA----EYDFELKYKKGEEHSNADGLSRL 1938 ME I + AK+I+ S A V KKDGS+R C+DYRGLN+IT+K ++ + + L A F+ +DL AY+ V + P + +TAF+ G F + L LS AP FQ L++ VL +M+ + + VYLDDILI+ S ++H + + + +R+ GL + EKC F+ + FLGH + E ++ + + + +FS + +S T +++ R+ S +F LK APIL P R F++E AS +G++ S V Y S YD + LL + + E R +L FI+ TDH ++ + K + + A A ++F + Y+ G ++ D LSRL Sbjct: 540 MEKYISDSLAAKIIRPSSSPAGAGFFFVKKKDGSLRPCIDYRGLNSITVKNTYPLPLMSSAFERLQGANFFTKLDLRNAYHLVCIRPGDEWKTAFNTPRGHFEYCVLPFGLSNAPAVFQALVNDVLRDMIDQFIYVYLDDILIFSHSLQEHIQHVRRVLQRLLENGLYVKAEKCVFHAQSVQFLGHIVSVEGMRMDPEKIQAVVNWPTPDSRKALQRFLGFANFYRRFIHNFS--QLAAPLTSLTSSKTPFRWSS----AAEAAFSKLKGCFVSAPILIAPDPSRQFVVEVDASEVGVGAILSQRSASDGKVHPCAYFSHRLSSAERNYDIGNRELLAVK--------LALEEWRHWLEGSGVPFIVWTDHKNLEYIKSAK-----RLNSRQARWALFFGRFNFTISYRPGSKNIKPDALSRL 943
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Zebrafish
Match: BX546500.1 (pep chromosome:GRCz11:23:12926092:12931693:-1 gene:ENSDARG00000086495.3 transcript:ENSDART00000122176.3 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX546500.1) HSP 1 Score: 138.272 bits (347), Expect = 4.184e-33 Identity = 119/433 (27.48%), Postives = 187/433 (43.19%), Query Frame = 1 Query: 772 MEDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNSXXXXXXXXXXXXXXXS*DHFSD*RTTTEDSSKTM------AESQNRFMS-------------------QHLYVTNESFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSH-------VKGICY-----SLWFETYDSV*KRLLYNEEKPSRCT*ICYEF*RIFLRK---KFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLA----EYDFELKYKKGEEHSNADGLSRL 1938 ME I + AK+I+ S A V KKDGS+R C+DYRGLN+IT+K ++ + + L A F+ +DL AY+ V + P + +TAF+ G F + L LS AP FQ L++ VL +M+ + + VYLDDILI+ S ++H + + + +R+ GL + EKC F+ + FLGH + E ++ + + + T DS K + A RF+ + +F LK APIL P R F++E AS +G++ S + Y S YD + LL + + E R +L FI+ TDH ++ + K + + A A ++F + Y+ G ++ D LSRL Sbjct: 514 MEKYISDSLAAKIIRPSSSPAGAGFFFVKKKDGSLRPCIDYRGLNSITVKNTYPLPLMSSAFERLQGANFFTKLDLRNAYHLVRIRPGDEWKTAFNTPRGHFEYCVLPFGLSNAPAVFQALVNDVLRDMIDQFIYVYLDDILIFSHSLQEHVQHVRRVLQRLLENGLYVKAEKCVFHAQSVQFLGHIVSVEGMRMDPEKIQAVVDW----------------PTPDSRKALQRFLGFANFYRRFIRNFSQLAAPLTSLTSSKMPFRWSSAAEAAFSKLKGCFVSAPILIAPDPSRQFVVEVDASEVGVGAILSQRSSSDGKIHPCAYFSHRLSPAERNYDIGNRELLAVK--------LALEEWRHWLEGSGVPFIVWTDHKNLEYIRSAK-----RLNSRQARWALFFGRFNFTISYRPGSKNIKPDALSRL 917
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Zebrafish
Match: CR749164.1 (pep chromosome:GRCz11:21:30355767:30361679:1 gene:ENSDARG00000116832.1 transcript:ENSDART00000189948.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR749164.1) HSP 1 Score: 130.568 bits (327), Expect = 1.085e-30 Identity = 113/426 (26.53%), Postives = 191/426 (44.84%), Query Frame = 1 Query: 739 IHGVNQCTEKRMEDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNSXXXXXXXXXXXXXXXS-----------*DHFSD*RTTTEDSSKTMAESQNRFMSQHLYVTNESFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSH---VKGICYSLWF---------ETYDSV*KRLLYNEEKPSRCT*ICYEF*RIFL---RKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSRL 1938 I ++Q + M+ I + ++ I+ S +A V KKDGS+R C+DYRGLN IT+K + + L+ L A F+ +DL AY + + + +T FS +G + + + L+ +P+ FQ ++ + +ML K V+VY+DDIL+Y S +H + + + KR+ L KCEF++T + FLG+ + E + + + Q Q F ++ M ++ N + + +F LK R APIL +P ++ F++E YAS IG++ S V + F YD + LL + E R +L + FI+ TDH ++ + K + P+ +DF++ Y G ++ AD LSRL Sbjct: 522 IFPLSQPETEAMKKYISEELEKGFIRPSTSPASAGFFFVKKKDGSLRPCIDYRGLNEITVKYRYPLPLVPAALEQLRSAQYFTKLDLRSAYNLIRIRQGDEWKTGFSTIDGHYEYLVMPFGLANSPSVFQAFVNEIFRDMLNKWVIVYIDDILVYSNSLSEHIQHVRAVLKRLIKNQLYAKSSKCEFHQTCISFLGYIISPEGVAMDQQKVDSVTQWPQPETIRQLQRFLGFANFYRRFIRNFSSVAAPLTAMVKANNARLKWNPDAV-RAFTQLKTRFSSAPILRHPDPEQPFVVEIYASNTGIGAILSQRSLVNKKLHPCAFYSRKLNSAERNYDVGNRELLAMK--------AALEEWRHWLEGAKHPFIVITDHKNLEYIRSCKR-LNPRQARWALFFTRFDFQVTYIPGSKNIKADALSRL 937
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Xenopus
Match: ENSXETT00000006041.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:6:37317867:37322724:1 gene:ENSXETG00000003070.1 transcript:ENSXETT00000006041.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 156.377 bits (394), Expect = 1.472e-43 Identity = 77/200 (38.50%), Postives = 129/200 (64.50%), Query Frame = 1 Query: 772 MEDL---IKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNS 1362 +EDL +++L A +I+ +S + + +V+V KK+GSIRMCVDYR LN T+ + ++ + L+CL + FS +DL YYQ+ + P+ +E+TAF G F FNR+ L AP TFQ LM + +M L V+VYLDD++++ ++ ++H++ L ++F R++ GLK++PEKC+F + + ++GH + E I T+ Sbjct: 339 LEDLRKHLEELKAAGIIKESRSPYASPIVVVRKKNGSIRMCVDYRTLNQRTIPDQYTTPRIEDALNCLVGSKWFSVLDLRSGYYQLPMHPEDKEKTAFICPLGFFEFNRMPQGLCGAPATFQRLMERTVGDMHLLEVLVYLDDLIVFGRTLEEHEQRLLKVFDRLEKEGLKLSPEKCQFCQPSVNYVGHVVSAEGIATDP 538 HSP 2 Score: 41.5874 bits (96), Expect = 1.472e-43 Identity = 15/41 (36.59%), Postives = 26/41 (63.41%), Query Frame = 3 Query: 1362 QVEEIEKAEEPKCSKQLRSFLRLTNYYRRFIKDYGRIAEPF 1484 ++E + +P+ +LRSFL YYRRF+K + ++A+P Sbjct: 540 KIEAVSSWPKPRTITELRSFLGFCGYYRRFVKGFSKVAQPL 580
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Xenopus
Match: anxa6 (annexin A6 [Source:Xenbase;Acc:XB-GENE-989741]) HSP 1 Score: 159.844 bits (403), Expect = 2.151e-40 Identity = 117/411 (28.47%), Postives = 200/411 (48.66%), Query Frame = 1 Query: 748 VNQCTEKRMEDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNSX--------XXXXXXXXXXXXXXS*DHFSD*RTTTEDSSKTMAESQNRFMSQHLYVTNE---SFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHVK--GICYSLWFETYDSV*KRLLYNE-EKPSRCT*ICYEF*RI---FLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGL 1929 V + M+ I +++ VI S W + +V+V KKDGS R CVDYR LN +T +++ M LLD L +A +++DL + Y+Q+ LAP QE++AF G + F + + AP TFQ L++ +LE M YLDDI ++ ++ ++H + L+ +F +IQ AGL + PEKC E+ +LGH ++ + +++ + ++ +K + + +R S+ + T E + LK L +P+L P R FIL+T AS +G+V S V G + + + + + + Y EK C I + ++ ++F + TDH ++ + L L +Y+F ++++KG+EH NADGL Sbjct: 345 VAEAVRPEMKSQIDEMLAFGVITPSHSPWASPVVLVPKKDGSTRFCVDYRRLNDVTTTDAYPMPRVDELLDRLGNAKYLTTLDLSRGYWQIPLAPSAQEKSAFLTPFGLYQFTVMPFGMRNAPATFQRLVNRLLEGM-QDFAQAYLDDIAVFSQTWEEHLQHLQRVFAQIQDAGLTLKPEKCHLAMAEVQYLGHRVGGGQLRPDPAKVEAICQWPIPKTQKQVLAFLGTSGYYRKFIPNYSTVAKPLTDLTSRQRSRTIVWTPECESAMNALKQALASSPVLAAPDFSRRFILQTDASNFGLGAVLSQVNTYGEEHPVAYLSRKLLPREAAYATIEK--ECLAIVWALQKLQPYLYGREFTVVTDHNPLSWLQRVSGDNGKLLRWSLL-LQQYNFTIQHRKGKEHHNADGL 751
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Xenopus
Match: alkbh5 (alkB homolog 5, RNA demethylase [Source:Xenbase;Acc:XB-GENE-987580]) HSP 1 Score: 129.798 bits (325), Expect = 4.885e-36 Identity = 73/221 (33.03%), Postives = 118/221 (53.39%), Query Frame = 1 Query: 670 DIIEKFGVKYDTRCGRQEVQ*CNIHGVNQCTEKRMEDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVV-VYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGH 1329 DII GVK + R R + + + + IK++++ VI+ S W++ +V+V K DGSIR C D+R LN ++ +++ M L++ L A +++DL + Y+QV L +E+TAFS EGQ+ + RL L AP TFQ +M +L+ +G YLDD++I+ + H ++ + I AGL NP+KC E +LG+ Sbjct: 227 DIITGPGVKVNVRPYR----------IPEARRQAVAGEIKRMLELDVIEESHSEWSSPIVLVPKPDGSIRFCNDFRKLNEVSKFDAYPMPRVDELIERLGQARYITTLDLTKGYWQVPLTEGAKEKTAFSTPEGQWQYKRLPFGLHGAPATFQRMMDRILKPH--RGYASAYLDDVVIFSPDWESHLPRVQAVLDSIWKAGLTANPKKCAMGLEEARYLGY 435 HSP 2 Score: 43.1282 bits (100), Expect = 4.885e-36 Identity = 16/45 (35.56%), Postives = 28/45 (62.22%), Query Frame = 3 Query: 1362 QVEEIEKAEEPKCSKQLRSFLRLTNYYRRFIKDYGRIAEPFYEST 1496 ++E I++ P KQ+R+FL + YYRRF+ ++ +A P + T Sbjct: 448 KIEGIQQWPRPNSKKQVRAFLGIVGYYRRFVPNFASLASPLTDLT 492
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Xenopus
Match: ENSXETT00000034712.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:9:33567219:33572531:-1 gene:ENSXETG00000011772.1 transcript:ENSXETT00000034712.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 115.931 bits (289), Expect = 5.868e-30 Identity = 65/197 (32.99%), Postives = 104/197 (52.79%), Query Frame = 1 Query: 772 MEDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNS 1362 M++ I + ++ I+ S A V KKDG +R C+DYRGLN IT+K + + L D L A IFS +DL AY + + + +TAF+ R+G + + + L AP FQ ++ + ++L K VVVYLDDILI+ + + H ++E R++ L EKC F ++ FLG+ + + + Sbjct: 536 MKEYISENLQRGFIRPSTSPAGAGFFFVEKKDGGLRPCIDYRGLNKITVKNRYPLPLISELFDQLKGAKIFSKLDLRGAYNLIRIREGDEWKTAFNTRDGHYEYLVMPFGLCNAPAVFQEFVNDIFRDLLGKSVVVYLDDILIFSQDLETHRSQVKEALSRLRENFLFAKLEKCTFEVPKISFLGYIISSRGFEMDP 732 HSP 2 Score: 36.5798 bits (83), Expect = 5.868e-30 Identity = 17/39 (43.59%), Postives = 26/39 (66.67%), Query Frame = 3 Query: 1362 QVEEIEKAEEPKCSKQLRSFLRLTNYYRRFIKDY-GRIA 1475 +V I+K P+ +K ++ F+ NYYR+FIKD+ RIA Sbjct: 734 KVSAIQKWPLPQSTKAIQRFIGFANYYRQFIKDFSSRIA 772
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Xenopus
Match: ENSXETT00000035398.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:3:9869556:9871940:-1 gene:ENSXETG00000011182.1 transcript:ENSXETT00000035398.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 117.857 bits (294), Expect = 8.589e-30 Identity = 65/197 (32.99%), Postives = 104/197 (52.79%), Query Frame = 1 Query: 772 MEDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNS 1362 M++ I + ++ I+ S A V KKDG +R C+DYRGLN IT+K + + L D L A IFS +DL AY + + + +TAF+ R+G + + + L AP FQ ++ + ++L K VVVYLDDILI+ + + H ++E R++ L EKC F ++ FLG+ + + + Sbjct: 18 MKEYISENLQRGFIRPSTSPAGAGFFFVEKKDGGLRPCIDYRGLNKITVKNRYPLPLISELFDQLKGAKIFSKLDLRGAYNLIRIREGDEWKTAFNTRDGHYEYLVMPFGLCNAPAVFQEFVNDIFRDLLGKSVVVYLDDILIFSQDLETHRSQVKEALSRLRENSLFAKLEKCTFEVPKISFLGYIISSRGFEMDP 214 HSP 2 Score: 33.8834 bits (76), Expect = 8.589e-30 Identity = 16/39 (41.03%), Postives = 25/39 (64.10%), Query Frame = 3 Query: 1362 QVEEIEKAEEPKCSKQLRSFLRLTNYYRRFIKDY-GRIA 1475 +V I+K P+ +K ++ F+ NYYR+FIK + RIA Sbjct: 216 KVSAIQKWPLPQSTKAIQRFIGFANYYRQFIKGFSSRIA 254
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. UniProt/SwissProt
Match: sp|P20825|POL2_DROME (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1) HSP 1 Score: 148.673 bits (374), Expect = 1.034e-35 Identity = 125/441 (28.34%), Postives = 211/441 (47.85%), Query Frame = 1 Query: 754 QCTEKRMEDLIKKLIKAKVIQRCKSSWNARLVIVGKK-DGS----IRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNSXXXXXXXXXXXXXXXS--------*DHFSD*RTTTEDSSKTMA---ESQNRFMSQHL-YVTNESFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHVKGICYSLWFET-------YDSV*KRLLYNEEKPSRCT*ICYEF*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSRLKGMICTKRQTRHEESKEGKSNI 2004 Q E +E+ +++++ +I+ S +N+ +V KK D S R+ +DYR LN IT+ + + + N +L L F++IDL + ++Q+E+ + +TAFS + G + + R+ L AP TFQ M+ +L +L K +VYLDDI+I+ S +H ++ +F ++ A LK+ +KCEF K E FLGH + I+ N K ++ ++ D +K M + + + +Q L Y+ E+F LK + PIL P ++ F+L T AS +G+V S G S T Y ++ K LL F L ++F+I +DH +++ K P + + L+EY F++ Y KG+E+S AD LSR+K + ++E SN+ Sbjct: 216 QTHEIEVENQVQEMLNQGLIRESNSPYNSPTWVVPKKPDASGANKYRVVIDYRKLNEITIPDRYPIPNMDEILGKLGKCQYFTTIDLAKGFHQIEMDEESISKTAFSTKSGHYEYLRMPFGLRNAPATFQRCMNNILRPLLNKHCLVYLDDIIIFSTSLTEHLNSIQLVFTKLADANLKLQLDKCEFLKKEANFLGHIVTPDGIKPNPIKVKAIVSYPIPTKDKEIRAFLGLTGYYRKFIPNYADIAKPMTSCLKKRTKIDTQKLEYI--EAFEKLKALIIRDPILQLPDFEKKFVLTTDASNLALGAVLSQ-NGHPISFISRTLNDHELNYSAIEKELL-------AIVWATKTFRHYLLGRQFLIASDHQPLRWLHNLKEPG-AKLERWRVRLSEYQFKIDYIKGKENSVADALSRIKIEENHHSEATQHSAEEDNSNL 645
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. UniProt/SwissProt
Match: sp|P04323|POL3_DROME (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1) HSP 1 Score: 145.976 bits (367), Expect = 8.859e-35 Identity = 122/420 (29.05%), Postives = 199/420 (47.38%), Query Frame = 1 Query: 754 QCTEKRMEDLIKKLIKAKVIQRCKSSWNARLVIVGKK-DGS----IRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNSXXXXXXXXXXXXXXXS--------*DHFSD*RTTTEDSSKTMAESQNRFMSQHLYVTNE----SFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHVKGICYSLWFET-------YDSV*KRLLYNEEKPSRCT*ICYEF*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSRLK 1941 Q E+ +E I+ ++ +I+ S +N+ + +V KK D S R+ +DYR LN IT+ + + N +L L F++IDL + ++Q+E+ P+ +TAFS + G + + R+ L AP TFQ M+ +L +L K +VYLDDI+++ S +H + L +F+++ A LK+ +KCEF K E FLGH + I+ N + + ++ + ++ D +K M + + M + TN +F LK + PIL P + F L T AS +G+V S G S T Y ++ K LL F L + F I +DH ++ K P + L+E+DF++KY KG+E+ AD LSR+K Sbjct: 217 QAYEQEVESQIQDMLNQGIIRTSNSPYNSPIWVVPKKQDASGKQKFRIVIDYRKLNEITVGDRHPIPNMDEILGKLGRCNYFTTIDLAKGFHQIEMDPESVSKTAFSTKHGHYEYLRMPFGLKNAPATFQRCMNDILRPLLNKHCLVYLDDIIVFSTSLDEHLQSLGLVFEKLAKANLKLQLDKCEFLKQETTFLGHVLTPDGIKPNPEKIEAIQKYPIPTKPKEIKAFLGLTGYYRKFIPNFADIAKPMTKCLKKNMK--IDTTNPEYDSAFKKLKYLISEDPILKVPDFTKKFTLTTDASDVALGAVLSQ-DGHPLSYISRTLNEHEINYSTIEKELL-------AIVWATKTFRHYLLGRHFEISSDHQPLSWLYRMKDPN-SKLTRWRVKLSEFDFDIKYIKGKENCVADALSRIK 625
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. UniProt/SwissProt
Match: sp|Q99315|YG31B_YEAST (Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-G PE=1 SV=3) HSP 1 Score: 136.346 bits (342), Expect = 1.296e-31 Identity = 121/445 (27.19%), Postives = 212/445 (47.64%), Query Frame = 1 Query: 658 IGGIDIIEKFGVKYDTRCGRQEVQ*CNIHGVNQCTEKRMEDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFN----------CESIQTNSXXXXXXXXXXXXXXXS*DHFSD*RTTTEDSSKTMAESQNRFM---SQHLYVTNESFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHVK------GICYSLWFETYDSV*KRLLYNEEKPSRCT*ICYEF*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSR 1935 I I + +K R R + + V + E+ + +++KL+ K I KS ++ +V+V KKDG+ R+CVDYR LN T+ + F + LL + +A IF+++DL Y+Q+ + P + +TAF G++ + + L AP+TF M + L+ V VYLDDILI+ +S ++H + L+ + +R++ L + +KC+F E FLG++ C +I+ K +++ + +++ R + SK +A+ F+ SQ +++ LK+ LC +P+L + L T AS IG+V V G+ + ++ +S K E + + F + K F +RTDH + + + P + Q L +LA YDF L+Y G ++ AD +SR Sbjct: 579 INNIPVKHDIEIKPGARLPRLQP-----YHVTEKNEQEINKIVQKLLDNKFIVPSKSPCSSPVVLVPKKDGTFRLCVDYRTLNKATISDPFPLPRIDNLLSRIGNAQIFTTLDLHSGYHQIPMEPKDRYKTAFVTPSGKYEYTVMPFGLVNAPSTFARYMADTFRD--LRFVNVYLDDILIFSESPEEHWKHLDTVLERLKNENLIVKKKKCKFASEETEFLGYSIGIQKIAPLQHKCAAIRDFPTPKTVKQAQRFLGMI--NYY---RRFIPNCSK-IAQPIQLFICDKSQWTEKQDKAIDKLKDALCNSPVLVPFNNKANYRLTTDASKDGIGAVLEEVDNKNKLVGVV-GYFSKSLESAQKNYPAGELELLGIIKALHHFRYMLHGKHFTLRTDHISLLSLQNKNEPA-RRVQRWLDDLATYDFTLEYLAGPKNVVADAISR 1008
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. UniProt/SwissProt
Match: sp|Q7LHG5|YI31B_YEAST (Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-I PE=1 SV=2) HSP 1 Score: 135.576 bits (340), Expect = 2.035e-31 Identity = 121/445 (27.19%), Postives = 211/445 (47.42%), Query Frame = 1 Query: 658 IGGIDIIEKFGVKYDTRCGRQEVQ*CNIHGVNQCTEKRMEDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFN----------CESIQTNSXXXXXXXXXXXXXXXS*DHFSD*RTTTEDSSKTMAESQNRFM---SQHLYVTNESFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHVK------GICYSLWFETYDSV*KRLLYNEEKPSRCT*ICYEF*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSR 1935 I I + +K R R + + V + E+ + +++KL+ K I KS ++ +V+V KKDG+ R+CVDYR LN T+ + F + LL + +A IF+++DL Y+Q+ + P + +TAF G++ + + L AP+TF M + L+ V VYLDDILI+ +S ++H + L+ + +R++ L + +KC+F E FLG++ C +I+ K +++ + +++ R + SK +A+ F+ SQ +++ LK LC +P+L + L T AS IG+V V G+ + ++ +S K E + + F + K F +RTDH + + + P + Q L +LA YDF L+Y G ++ AD +SR Sbjct: 605 INNIPVKHDIEIKPGARLPRLQP-----YHVTEKNEQEINKIVQKLLDNKFIVPSKSPCSSPVVLVPKKDGTFRLCVDYRTLNKATISDPFPLPRIDNLLSRIGNAQIFTTLDLHSGYHQIPMEPKDRYKTAFVTPSGKYEYTVMPFGLVNAPSTFARYMADTFRD--LRFVNVYLDDILIFSESPEEHWKHLDTVLERLKNENLIVKKKKCKFASEETEFLGYSIGIQKIAPLQHKCAAIRDFPTPKTVKQAQRFLGMI--NYY---RRFIPNCSK-IAQPIQLFICDKSQWTEKQDKAIEKLKAALCNSPVLVPFNNKANYRLTTDASKDGIGAVLEEVDNKNKLVGVV-GYFSKSLESAQKNYPAGELELLGIIKALHHFRYMLHGKHFTLRTDHISLLSLQNKNEPA-RRVQRWLDDLATYDFTLEYLAGPKNVVADAISR 1034
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. UniProt/SwissProt
Match: sp|P10394|POL4_DROME (Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaster OX=7227 GN=POL PE=4 SV=1) HSP 1 Score: 106.686 bits (265), Expect = 1.898e-29 Identity = 62/188 (32.98%), Postives = 101/188 (53.72%), Query Frame = 1 Query: 784 IKKLIKAKVIQRCKSSWNARLVIVGKKDGS------IRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGH 1329 ++KLIK K+++ S +N+ L++V KK R+ +DYR +N L + F + +LD L A FS +DL ++Q+EL ++ T+FS G + F RL L AP +FQ +M + +Y+DD+++ S+K + L E+F + + LK++PEKC F E+ FLGH Sbjct: 334 VQKLIKDKIVEPSVSQYNSPLLLVPKKSSPNSDKKKWRLVIDYRQINKKLLADKFPLPRIDDILDQLGRAKYFSCLDLMSGFHQIELDEGSRDITSFSTSNGSYRFTRLPFGLKIAPNSFQRMMTIAFSGIEPSQAFLYMDDLIVIGCSEKHMLKNLTEVFGKCREYNLKLHPEKCSFFMHEVTFLGH 521 HSP 2 Score: 38.1206 bits (87), Expect = 1.898e-29 Identity = 38/147 (25.85%), Postives = 65/147 (44.22%), Query Frame = 1 Query: 1513 ESFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWS-----HVKGICYSLWFETYDSV*KRLLYNEEKPSRCT*ICYEF*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSRL 1938 ++FI LK++L +L YP + F + T AS G+V + H + Y+ T K E++ + F K F ++TDH ++ + P + L EY+F ++Y KG+++ AD LSR+ Sbjct: 592 KAFIHLKSQLINPTLLQYPDFSKEFCITTDASKQACGAVLTQNHNGHQLPVAYASRAFTKGESNKST--TEQELAAIHWAIIHFRPYIYGKHFTVKTDHRPLTYLFSMVNPS-SKLTRIRLELEEYNFTVEYLKGKDNHVADALSRI 735 HSP 3 Score: 27.7202 bits (60), Expect = 1.898e-29 Identity = 12/31 (38.71%), Postives = 17/31 (54.84%), Query Frame = 3 Query: 1374 IEKAEEPKCSKQLRSFLRLTNYYRRFIKDYG 1466 I+ P + R F+ NYYRRFIK++ Sbjct: 538 IQNYPVPHDADSARRFVAFCNYYRRFIKNFA 568
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. TrEMBL
Match: A0A4Y2PDL7 (Retrovirus-related Pol polyprotein from transposon 297 (Fragment) OS=Araneus ventricosus OX=182803 GN=pol_1756 PE=4 SV=1) HSP 1 Score: 162.54 bits (410), Expect = 2.611e-57 Identity = 75/196 (38.27%), Postives = 125/196 (63.78%), Query Frame = 1 Query: 775 EDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNS 1362 E L+K+++ +I+ W + +V+V KKDGS R CVDYR LN IT+K+S+ + LD L + FS++DL Y+QVE+ P+ +E+TAF+ +G + F + L AP TF+ LM TVL + + +VYLDDI+I ++ ++H + ++F+R+Q A LK++P+KC F + E+ +LGH + + ++T+ Sbjct: 718 ERLVKEMVDNGIIEESSGPWASPIVLVKKKDGSTRFCVDYRKLNEITIKDSYPLPRIDDTLDALNGSQWFSTLDLKSGYWQVEIQPEDKEKTAFTTGQGLWQFKVMPFGLCNAPATFERLMETVLRGLTSEACLVYLDDIIIVGRTFQEHLNNIRKVFQRLQKANLKLSPKKCRFFRKEVSYLGHIISADGVKTDP 913 HSP 2 Score: 72.4034 bits (176), Expect = 2.611e-57 Identity = 65/194 (33.51%), Postives = 88/194 (45.36%), Query Frame = 1 Query: 1519 FIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHVKGI--CYSLWFETYDSV*KRLLYNEEKPSRCT*ICYE-----------F*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSRLKGMICTKRQTRHEESKEGKSNIRYVYSIKRTETMEKYWSSS 2061 F LK L P+L YPR D+ FIL+T AS IG+V S G C +F K L KP R + + F +KF++RTDH + ++ + P Q + L EYDFE++++KG H NAD LSR C K +H + E K + S+K T E WSSS Sbjct: 975 FNSLKQALITPPVLTYPRTDKEFILDTDASNEGIGAVLSQKIGNEECVIAYFS------KSL----GKPERNYCVTRKELLAIVKSIEHFHHYLYGRKFLLRTDHASLRWLLNFREPE-GQIARWIQRLQEYDFEIQHRKGTSHGNADALSRRP---C-KENCKHCTNAEKKFGMETDISVK-VLTTEDAWSSS 1152 HSP 3 Score: 39.6614 bits (91), Expect = 2.611e-57 Identity = 20/48 (41.67%), Postives = 29/48 (60.42%), Query Frame = 3 Query: 1392 PKCSKQLRSFLRLTNYYRRFIKDYGRIAEPFYESTSVCDK*KFHSTEE 1535 P+ LRSFL L YYRRF++++ IA P ++ T + F+ TEE Sbjct: 925 PETVHDLRSFLGLCTYYRRFVRNFSAIARPLHKLTEA--RSNFNWTEE 970
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. TrEMBL
Match: A0A4Y2LPA5 (Transposon Ty3-I Gag-Pol polyprotein OS=Araneus ventricosus OX=182803 GN=TY3B-I_314 PE=4 SV=1) HSP 1 Score: 162.54 bits (410), Expect = 2.838e-57 Identity = 75/196 (38.27%), Postives = 125/196 (63.78%), Query Frame = 1 Query: 775 EDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNS 1362 E L+K+++ +I+ W + +V+V KKDGS R CVDYR LN IT+K+S+ + LD L + FS++DL Y+QVE+ P+ +E+TAF+ +G + F + L AP TF+ LM TVL + + +VYLDDI+I ++ ++H + ++F+R+Q A LK++P+KC F + E+ +LGH + + ++T+ Sbjct: 282 ERLVKEMVDNGIIEESSGPWASPIVLVKKKDGSTRFCVDYRKLNEITIKDSYPLPRIDDTLDALNGSQWFSTLDLKSGYWQVEIQPEDKEKTAFTTGQGLWQFKVMPFGLCNAPATFERLMETVLRGLTSEACLVYLDDIIIVGRTFQEHLNNIRKVFQRLQKANLKLSPKKCRFFRKEVSYLGHIISADGVKTDP 477 HSP 2 Score: 72.4034 bits (176), Expect = 2.838e-57 Identity = 66/196 (33.67%), Postives = 91/196 (46.43%), Query Frame = 1 Query: 1513 ESFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHVKGI--CYSLWFETYDSV*KRLLYNEEKPSRCT*ICYE-----------F*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSRLKGMICTKRQTRHEESKEGKSNIRYVYSIKRTETMEKYWSSS 2061 +SF LK L +P+L YPR D+ FIL+T AS IG+V S G C +F K L KP R + + F +KF++RTDH + ++ + P Q + L EYDFE++++KG H NAD LSR C K +H + E K + S+K T E WSSS Sbjct: 537 KSFNSLKQALITSPVLTYPRTDKEFILDTDASNEGIGAVLSQKIGNEECVIAYFS------KSL----GKPERNYCVTRKELLAIVKSIEHFHHYLYGRKFLLRTDHASLRWLLNFREPE-GQIARWIQRLQEYDFEIQHRKGTSHGNADALSRRP---C-KENCKHCTNAEKKFGMETDISVK-VLTTEDAWSSS 716 HSP 3 Score: 39.6614 bits (91), Expect = 2.838e-57 Identity = 20/48 (41.67%), Postives = 29/48 (60.42%), Query Frame = 3 Query: 1392 PKCSKQLRSFLRLTNYYRRFIKDYGRIAEPFYESTSVCDK*KFHSTEE 1535 P+ LRSFL L YYRRF++++ IA P ++ T + F+ TEE Sbjct: 489 PETVHDLRSFLGLCTYYRRFVRNFSAIARPLHKLTEA--RTNFNWTEE 534
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. TrEMBL
Match: A0A4Y2F7R8 (Transposon Ty3-I Gag-Pol polyprotein OS=Araneus ventricosus OX=182803 GN=TY3B-I_165 PE=4 SV=1) HSP 1 Score: 162.925 bits (411), Expect = 7.605e-57 Identity = 75/196 (38.27%), Postives = 125/196 (63.78%), Query Frame = 1 Query: 775 EDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNS 1362 E L+K+++ +I+ W + +V+V KKDGS R CVDYR LN IT+K+S+ + LD L + FS++DL Y+QVE+ P+ +E+TAF+ +G + F + L AP TF+ LM TVL + + +VYLDDI+I ++ ++H + ++F+R+Q A LK++P+KC F + E+ +LGH + + ++T+ Sbjct: 574 ERLVKEMVDNGIIEESSGPWASPIVLVKKKDGSTRFCVDYRKLNEITIKDSYPLPRIDDTLDALNGSQWFSTLDLKSGYWQVEIQPEDKEKTAFTTGQGLWQFKVMPFGLCNAPATFERLMETVLRGLTSEACLVYLDDIIIVGRTFQEHLNNIRKVFQRLQKANLKLSPKKCRFFRKEVSYLGHIISADGVKTDP 769 HSP 2 Score: 72.0182 bits (175), Expect = 7.605e-57 Identity = 65/197 (32.99%), Postives = 91/197 (46.19%), Query Frame = 1 Query: 1513 ESFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHVKGI--CYSLWFETYDSV*KRLLYNEEKPSRCT*ICYE-----------F*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSRLKGMICTKRQTRHEESKEGKSNIRYVYSIKRTETMEKYWSSSR 2064 +SF LK L +P+L YPR D+ FIL+T AS IG+V S G C +F K L KP R + + F +KF++RTDH + ++ + P Q + L EYDFE++++KG H NAD LSR C K +H + E K + S+K T E WS +R Sbjct: 829 KSFNSLKQALITSPVLTYPRTDKEFILDTDASNEGIGAVLSQKIGNEECVIAYFS------KSL----GKPERNYCVTRKELLAIVKSIEHFHHYLYGRKFLLRTDHASLRWLLNFREPE-GQIARWIQRLQEYDFEIQHRKGTSHGNADALSRRP---C-KESCKHCTNAEKKFGMETDISVK-VLTTEDAWSPNR 1009 HSP 3 Score: 38.5058 bits (88), Expect = 7.605e-57 Identity = 19/48 (39.58%), Postives = 29/48 (60.42%), Query Frame = 3 Query: 1392 PKCSKQLRSFLRLTNYYRRFIKDYGRIAEPFYESTSVCDK*KFHSTEE 1535 P+ +RSFL L YYRRF++++ IA P ++ T + F+ TEE Sbjct: 781 PETVHDVRSFLGLCTYYRRFVRNFSAIARPLHKLTEA--RSNFNWTEE 826
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. TrEMBL
Match: A0A4Y2GV68 (Transposon Ty3-I Gag-Pol polyprotein OS=Araneus ventricosus OX=182803 GN=TY3B-I_1030 PE=4 SV=1) HSP 1 Score: 162.925 bits (411), Expect = 1.195e-56 Identity = 75/196 (38.27%), Postives = 125/196 (63.78%), Query Frame = 1 Query: 775 EDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNS 1362 E L+K+++ +I+ W + +V+V KKDGS R CVDYR LN IT+K+S+ + LD L + FS++DL Y+QVE+ P+ +E+TAF+ +G + F + L AP TF+ LM TVL + + +VYLDDI+I ++ ++H + ++F+R+Q A LK++P+KC F + E+ +LGH + + ++T+ Sbjct: 720 ERLVKEMVDNGIIEESSGPWASPIVLVKKKDGSTRFCVDYRKLNEITIKDSYPLPRIDDTLDALNGSQWFSTLDLKSGYWQVEIQPEDKEKTAFTTGQGLWQFKVMPFGLCNAPATFERLMETVLRGLTSEACLVYLDDIIIVGRTFQEHINNIRKVFQRLQKANLKLSPKKCRFFRKEVSYLGHIISADGVKTDP 915 HSP 2 Score: 69.707 bits (169), Expect = 1.195e-56 Identity = 53/154 (34.42%), Postives = 74/154 (48.05%), Query Frame = 1 Query: 1513 ESFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHVKGI--CYSLWFETYDSV*KRLLYNEEKPSRCT*ICYE-----------F*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSR 1935 +SF LK L +P+L YPR D+ FIL+T AS IG+V S G C +F K L KP R + + F +KF++RTDH + ++ + P Q + L EYDFE++++KG H NAD LSR Sbjct: 975 KSFNSLKQALITSPVLTYPRTDKEFILDTDASNEGIGAVLSQKIGNEECVIAYFS------KSL----GKPERNYCVTRKELLAIVKSIEHFHHYLYGRKFLLRTDHASLRWLLNFREPE-GQIARWIQRLQEYDFEIQHRKGTSHGNADALSR 1117 HSP 3 Score: 40.0466 bits (92), Expect = 1.195e-56 Identity = 20/48 (41.67%), Postives = 29/48 (60.42%), Query Frame = 3 Query: 1392 PKCSKQLRSFLRLTNYYRRFIKDYGRIAEPFYESTSVCDK*KFHSTEE 1535 P+ LRSFL L YYRRF++++ IA P ++ T + F+ TEE Sbjct: 927 PETVHDLRSFLGLCTYYRRFVRNFSAIARPLHKLTEA--RTNFNWTEE 972
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. TrEMBL
Match: A0A4Y1ZYF3 (Transposon Ty3-I Gag-Pol polyprotein OS=Araneus ventricosus OX=182803 GN=TY3B-I_1211 PE=4 SV=1) HSP 1 Score: 167.548 bits (423), Expect = 1.205e-56 Identity = 77/196 (39.29%), Postives = 128/196 (65.31%), Query Frame = 1 Query: 775 EDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNS 1362 E LIK+++ ++I+ W + +V+V KKDGS R CVDYR LN IT+K+S+ + LD L + FS++DL Y+QVE+ P+ +E+TAF+ +G + F + L AP TF+ LM TVL E+ + +VYLDDI+I ++ ++H + + ++F+R+Q A LK++P+KC F + E+ +LGH + + ++T+ Sbjct: 30 ERLIKEMVDNRIIEESSGPWASPIVLVKKKDGSTRFCVDYRKLNEITIKDSYPLPRIDDTLDALNGSQWFSTLDLKSGYWQVEIQPEDKEKTAFTTGQGLWQFKVMPFGLCNAPATFERLMETVLRELTSEACLVYLDDIIIAGRTFQEHLDNIRKVFQRLQKANLKLSPKKCRFFRKEVSYLGHIISADGVKTDP 225 HSP 2 Score: 65.4698 bits (158), Expect = 1.205e-56 Identity = 65/196 (33.16%), Postives = 90/196 (45.92%), Query Frame = 1 Query: 1513 ESFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHVKGI--CYSLWFETYDSV*KRLLYNEEKPSRCT*ICYE-----------F*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSRLKGMICTKRQTRHEESKEGKSNIRYVYSIKRTETMEKYWSSS 2061 +SF LK L +P+L Y R D+ FIL+T AS IG+V S G C +F K L KP R + + F +KF++RTDH + ++ + P Q + L EYDFE++++KG H NAD LSR C K +H + E K + S+K T E WSSS Sbjct: 285 KSFNSLKQTLITSPVLTYLRTDKEFILDTDASNEGIGAVLSQKIGNEECVIAYFS------KSL----GKPERNYCVTRKELLAIVKSIEHFHHYLYGRKFLLRTDHASLRWLLNFREPE-GQIARWIQRLQEYDFEIQHRKGTSHGNADALSRRP---C-KENCKHCTNAEKKFGMETDISVK-VLTTEDAWSSS 464 HSP 3 Score: 39.6614 bits (91), Expect = 1.205e-56 Identity = 20/48 (41.67%), Postives = 29/48 (60.42%), Query Frame = 3 Query: 1392 PKCSKQLRSFLRLTNYYRRFIKDYGRIAEPFYESTSVCDK*KFHSTEE 1535 P+ LRSFL L YYRRF++++ IA P ++ T + F+ TEE Sbjct: 237 PETVHDLRSFLGLCTYYRRFVRNFSAIARPLHKLTEA--RSNFNWTEE 282
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Cavefish
Match: ENSAMXT00000034038.1 (pep primary_assembly:Astyanax_mexicanus-2.0:14:3120146:3124090:1 gene:ENSAMXG00000040885.1 transcript:ENSAMXT00000034038.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 147.902 bits (372), Expect = 4.811e-48 Identity = 71/193 (36.79%), Postives = 124/193 (64.25%), Query Frame = 1 Query: 784 IKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNS 1362 I ++ +A VI+ KS + + +V+V KKDGS+R+C+DYR LN+ + +++F + + L+ L +A FS++DL Y+QVE+A + +TAFS G F NR+ L +P+TFQ LM ++ + +++YLDDI+I+ ++ +H E L+ +F R++ GLK+ P+KC + E+ +LGH + + I+T+ Sbjct: 258 ITEMEEAGVIRPSKSPYASPIVVVTKKDGSLRICMDYRKLNSCSTRDAFPLPRVEEALEALGEAKYFSTLDLTSGYWQVEVAEADKHKTAFSTPMGLFEANRMPFGLQNSPSTFQRLMTCCFGDLNFESLLIYLDDIVIFSRTFDEHLERLQMVFDRLRKYGLKLKPQKCHLLRREVLYLGHVVSSDGIKTDP 450 HSP 2 Score: 47.3654 bits (111), Expect = 4.811e-48 Identity = 40/156 (25.64%), Postives = 73/156 (46.79%), Query Frame = 1 Query: 1516 SFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHVKGICYSLWFETYDSV*KRLLYNEE--KPSRCT*ICYEF*RIFLR-------------KKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSRL 1938 +F LK +L AP+L Y + F+L+T AS +G+V + V+ D + + Y P+ ++ + L+ KF + TD+ ++ TT + Q +A L+ +DF+++Y++G ++NADGLSR+ Sbjct: 526 AFQTLKTKLTTAPVLGYADYSQPFVLQTDASLAGLGAVLAQVQ-----------DGRERVIAYASRGLNPAETRYPAHKLEFLALKWAVTDKFYDHLYGHKFSVLTDNNPLKYVMTTAK-LDATGQRWVAQLSMFDFDIRYRQGGCNANADGLSRM 669 HSP 3 Score: 37.3502 bits (85), Expect = 4.811e-48 Identity = 17/46 (36.96%), Postives = 25/46 (54.35%), Query Frame = 3 Query: 1362 QVEEIEKAEEPKCSKQLRSFLRLTNYYRRFIKDYGRIAEPFYESTS 1499 ++ ++ + P+ +L FL YYRRFI+ Y IA P Y TS Sbjct: 452 KISKVAGWKVPENRHELLQFLGFAGYYRRFIEGYASIAAPLYRLTS 497
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Cavefish
Match: ENSAMXT00000052546.1 (pep primary_assembly:Astyanax_mexicanus-2.0:2:13031363:13036570:1 gene:ENSAMXG00000033629.1 transcript:ENSAMXT00000052546.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 144.05 bits (362), Expect = 3.890e-47 Identity = 73/193 (37.82%), Postives = 116/193 (60.10%), Query Frame = 1 Query: 784 IKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNS 1362 ++ L+ +++ S W + VIV KKDG++R C DYR LN +T K+++ + + LD L +A FS++DL Y+QV + +E+TA + G F ++R+ L AP TFQ LM VL ++ ++VYLDDI+I+ + K H E LE +F R++ GLK+ P KC K E+ FLGH + + +Q ++ Sbjct: 742 VQSLVDRGILEESCSPWASPAVIVIKKDGTVRFCCDYRRLNQVTCKDAYPLPRVEESLDALGNAQWFSTLDLTAGYFQVAVKDSDREKTAVTTPFGLFQWSRMPFGLCNAPATFQRLMGVVLGDLAFDILLVYLDDIIIFSRDFKSHCERLELVFNRLRHHGLKLKPSKCFLLKPEVKFLGHIISAKGVQVDA 934 HSP 2 Score: 52.373 bits (124), Expect = 3.890e-47 Identity = 54/201 (26.87%), Postives = 82/201 (40.80%), Query Frame = 1 Query: 1516 SFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHVKG-----ICYSLWFETYDSV*KRLLYNEEKPSRC------------T*ICYEF*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSRLKGMICTKRQTRHEESKEGKSNIRYVYSIKRTETMEKYWSSSRR 2067 +F LK L P+L YP + FIL T S +G+V S +G I Y+ R L EK + + +F + KF + TDH ++++ + Q +A +AEY+F++ YK G +++NAD LSRL HEE E K + I E W ++ R Sbjct: 1002 AFDKLKGCLMSPPVLAYPDFTQPFILTTDGSLHGLGAVLSQKQGGTERVIAYA----------SRGLRGSEKNDKNYSAFKLELLALKWAVTEKFRDFLMFAKFSVVTDHNPLRYLDSANLGVVEQRW--VAQMAEYNFDISYKPGRQNANADVLSRLPA---------HEEP-EAKDTGKDFILINTEEVRACLWPAAER 1180 HSP 3 Score: 33.4982 bits (75), Expect = 3.890e-47 Identity = 14/42 (33.33%), Postives = 24/42 (57.14%), Query Frame = 3 Query: 1362 QVEEIEKAEEPKCSKQLRSFLRLTNYYRRFIKDYGRIAEPFY 1487 +V +E PK K +R L +YYRRF+ + ++A+P + Sbjct: 936 KVRVLETWPIPKTVKDIRQVLGFMSYYRRFVPKFAQLAKPLH 977
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Cavefish
Match: ENSAMXT00000048318.1 (pep primary_assembly:Astyanax_mexicanus-2.0:14:10030146:10034843:1 gene:ENSAMXG00000044052.1 transcript:ENSAMXT00000048318.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 147.902 bits (372), Expect = 1.143e-46 Identity = 70/193 (36.27%), Postives = 129/193 (66.84%), Query Frame = 1 Query: 784 IKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNS 1362 +++L++A VI+ +SS+++ +V+V KK+G +R+CVDYR LN T+K+++++ N + LT + FS +DL YYQ+EL + +TAF G + FN+L ++ AP+TFQ LM + ++ LK V+V+LDD++++ ++ ++H+ L + +R++ GLK++PEKC F +T + +LGH + ++T+ Sbjct: 357 LEELLEAGVIRESESSFSSPIVVVRKKNGDVRLCVDYRKLNLQTVKDAYALPNLEETFSVLTGSQWFSVLDLKSGYYQIELNEADKHKTAFVCPLGFWEFNKLPQGITNAPSTFQRLMEKCMSDLNLKEVIVFLDDLIVFSETLEEHEHRLLRVLQRLKEYGLKLSPEKCRFFQTSVRYLGHIVSRNGVETDP 549 HSP 2 Score: 45.4394 bits (106), Expect = 1.143e-46 Identity = 19/46 (41.30%), Postives = 30/46 (65.22%), Query Frame = 3 Query: 1362 QVEEIEKAEEPKCSKQLRSFLRLTNYYRRFIKDYGRIAEPFYESTS 1499 ++E ++ PK K+LRSFL YYRRFI+D+ +I +P + T+ Sbjct: 551 KIEALKNWPNPKNLKELRSFLGFAGYYRRFIQDFSKIVKPLNDLTA 596 HSP 3 Score: 35.039 bits (79), Expect = 1.143e-46 Identity = 36/152 (23.68%), Postives = 70/152 (46.05%), Query Frame = 1 Query: 1513 ESFIVLKNRLCVAPILDYPREDRTFILETYAS-FGIIGSVWSHVKGICYSLWFETYDSV*KRLLYNEEK-PSRCT-------*ICYEF*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSRLK 1941 E+F + N+L AP+L Y +IL T AS G+ +++ G ++ F + + L ++E + P+ + +F F + TD ++ T+ ++ L+ L+ + F+L+Y+ G+ + +AD LSRL Sbjct: 631 EAFDTIINKLTSAPVLAYANPKLPYILHTDASTTGLGAALYQEQNGQIRAIAFAS-----RGLSHSESRYPAHKLEFLALKWAVTEKFSDYLYGNSFTVITDSNPLTYILTSAKLDATSYRW-LSALSTFSFKLQYRPGKHNLDADALSRLP 776
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Cavefish
Match: ENSAMXT00000048050.1 (pep primary_assembly:Astyanax_mexicanus-2.0:19:20134742:20139355:-1 gene:ENSAMXG00000043312.1 transcript:ENSAMXT00000048050.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 150.599 bits (379), Expect = 2.515e-46 Identity = 72/193 (37.31%), Postives = 129/193 (66.84%), Query Frame = 1 Query: 784 IKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNS 1362 +++L++A VI+ +SS+++ +V+V KK+G +R+CVDYR LN T+K+++++ N + LT + FS +DL YYQ+EL + +TAF G + FNRL ++ AP+TFQ LM + ++ LK V+V+LDD++I+ + ++H+ L + +R++ GLK++PEKC+F +T + +LGH + ++T+ Sbjct: 357 LEELLEAGVIRESESSFSSPIVVVRKKNGDVRLCVDYRKLNLQTVKDAYALPNLEETFSVLTGSQWFSVLDLKSGYYQIELEEADKHKTAFVCPMGFWEFNRLPQGITNAPSTFQRLMEKCMSDLHLKEVIVFLDDLIIFSATLEEHEHRLLRVLQRLKEYGLKLSPEKCKFFQTSVRYLGHIVSRNGVETDP 549 HSP 2 Score: 45.8246 bits (107), Expect = 2.515e-46 Identity = 19/46 (41.30%), Postives = 30/46 (65.22%), Query Frame = 3 Query: 1362 QVEEIEKAEEPKCSKQLRSFLRLTNYYRRFIKDYGRIAEPFYESTS 1499 +++ ++ PK K+LRSFL YYRRFI+DY +I +P + T+ Sbjct: 551 KIQSLKNWPNPKNLKELRSFLGFAGYYRRFIQDYSKIVKPLNDLTA 596 HSP 3 Score: 30.4166 bits (67), Expect = 2.515e-46 Identity = 33/145 (22.76%), Postives = 63/145 (43.45%), Query Frame = 1 Query: 1513 ESFIVLKNRLCVAPILDYPREDRTFILETYAS-FGIIGSVWSHVKGICYSLWFETYDSV*KRLLYNEEKPSRCT---*ICYEF*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSR 1935 E+F + ++L AP+L Y +IL T AS G+ +++ G ++ F + Y K + +F F + TD ++ T+ ++ L+ L+ + F+L+Y+ G+ + +AD LSR Sbjct: 631 EAFNTIISKLTSAPVLAYANSQLPYILHTDASTTGLGAALYQEQDGQIRAIAFASRGLSRSESRYPAHKLEFLALKWAVTEKFSDYLYGNSFTVITDSNPLTYILTSAKLDATSYRW-LSALSTFSFKLQYRPGKHNLDADALSR 774
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Cavefish
Match: ENSAMXT00000047338.1 (pep primary_assembly:Astyanax_mexicanus-2.0:9:32004976:32009589:-1 gene:ENSAMXG00000038997.1 transcript:ENSAMXT00000047338.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 150.599 bits (379), Expect = 2.753e-46 Identity = 72/193 (37.31%), Postives = 129/193 (66.84%), Query Frame = 1 Query: 784 IKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNS 1362 +++L++A VI+ +SS+++ +V+V KK+G +R+CVDYR LN T+K+++++ N + LT + FS +DL YYQ+EL + +TAF G + FNRL ++ AP+TFQ LM + ++ LK V+V+LDD++I+ + ++H+ L + +R++ GLK++PEKC+F +T + +LGH + ++T+ Sbjct: 357 LEELLEAGVIRESESSFSSPIVVVRKKNGDVRLCVDYRKLNLQTVKDAYALPNLEETFSVLTGSQWFSVLDLKSGYYQIELEEADKHKTAFVCPMGFWEFNRLPQGITNAPSTFQRLMEKCMSDLHLKEVIVFLDDLIIFSATLEEHEHRLLRVLQRLKEYGLKLSPEKCKFFQTSVRYLGHIVSRNGVETDP 549 HSP 2 Score: 45.8246 bits (107), Expect = 2.753e-46 Identity = 19/46 (41.30%), Postives = 30/46 (65.22%), Query Frame = 3 Query: 1362 QVEEIEKAEEPKCSKQLRSFLRLTNYYRRFIKDYGRIAEPFYESTS 1499 +++ ++ PK K+LRSFL YYRRFI+DY +I +P + T+ Sbjct: 551 KIQSLKNWPNPKNLKELRSFLGFAGYYRRFIQDYSKIVKPLNDLTA 596 HSP 3 Score: 30.4166 bits (67), Expect = 2.753e-46 Identity = 33/145 (22.76%), Postives = 63/145 (43.45%), Query Frame = 1 Query: 1513 ESFIVLKNRLCVAPILDYPREDRTFILETYAS-FGIIGSVWSHVKGICYSLWFETYDSV*KRLLYNEEKPSRCT---*ICYEF*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSR 1935 E+F + ++L AP+L Y +IL T AS G+ +++ G ++ F + Y K + +F F + TD ++ T+ ++ L+ L+ + F+L+Y+ G+ + +AD LSR Sbjct: 631 EAFNTIISKLTSAPVLAYANSQLPYILHTDASTTGLGAALYQEQDGQIRAIAFASRGLSRSESRYPAHKLEFLALKWAVTEKFSDYLYGNSFTVITDSNPLTYILTSAKLDATSYRW-LSALSTFSFKLQYRPGKHNLDADALSR 774
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Medaka
Match: ENSORLT00000040305.1 (pep primary_assembly:ASM223467v1:1:25459511:25466649:-1 gene:ENSORLG00000028409.1 transcript:ENSORLT00000040305.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 187.963 bits (476), Expect = 2.440e-49 Identity = 131/407 (32.19%), Postives = 209/407 (51.35%), Query Frame = 1 Query: 784 IKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNSXXXXXXXXXXXXXXXS*DHFSD*RTTTEDSS---------KTMAESQNRFMSQHL---YVT--NESFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHVKGICYSLWFETYDSV*KRLLYNEEKPSRCT---------*ICYEF*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSR 1935 +++ ++A + + SSW A +V+V KKD S R+CVDYR LN T+K+++ + + LD L+ A FS++DL Y+QVE+ P ++ AF R+G F +N + L AP TFQ LM VL + + +VYLDDI++ + E LE++F R++ A LK+ P KC + ++ +LGH + + I T+ + + + + S+ R +S T+A + ++ + T E+F LK RL APIL YP + +L+T AS +G+V S V+G + Y S +RL E+ + CT F + L + F++RTDH + ++ K P Q L LAEYDF++ ++ G+ H NAD LSR Sbjct: 852 VQQSLEAGLARHSNSSWAAPIVMVKKKDQSPRLCVDYRPLNDRTIKDAYPLPRIQDTLDTLSTAKYFSTLDLTSGYWQVEMTPRARKAAAFCTRKGLFEWNVMPFGLCNAPATFQRLMDRVLAGLQWEACLVYLDDIIVLGCNSNQMMERLEQVFTRLRQANLKLKPSKCRLFQEQVAYLGHVVSAQGISTDPQKIQKVQDWPIPK-----NISEVRQFVGLASYYRRFVADFATIARPLHELTKKYARFDWTTECQEAFEELKERLTSAPILGYPLDSGELLLDTDASDWGVGAVLSQVQGGEERVL--AYGS--RRLTTTEQ--NYCTTRRELLAVVEFTSHFRQYLLGRSFVLRTDHSSLRWLTRLKEPE-GQLARWLEKLAEYDFQVLHRPGKVHQNADALSR 1246 HSP 2 Score: 52.373 bits (124), Expect = 1.798e-6 Identity = 26/54 (48.15%), Postives = 34/54 (62.96%), Query Frame = 2 Query: 1643 KESVTAYGLRHMTAYEKGYCIMRKNLLAVHEYVMNFREYFYGKSL*FVQITMHS 1804 +E V AYG R +T E+ YC R+ LLAV E+ +FR+Y G+S FV T HS Sbjct: 1150 EERVLAYGSRRLTTTEQNYCTTRRELLAVVEFTSHFRQYLLGRS--FVLRTDHS 1201
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Medaka
Match: ENSORLT00000045600.1 (pep primary_assembly:ASM223467v1:4:26167161:26171153:-1 gene:ENSORLG00000023514.1 transcript:ENSORLT00000045600.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 151.754 bits (382), Expect = 1.208e-46 Identity = 75/193 (38.86%), Postives = 120/193 (62.18%), Query Frame = 1 Query: 784 IKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNS 1362 I++L++++VI+ S + + +V+V KKDG +RMCVDYR LNA T K++F + LD L A F+++DL Y QVE+A + +TAF G F FNR+ L AP+TFQ LM + + + V++YLDD++I+ S + H + LE++F R++ GLK+ KC F + ++ +LGH + E + T+ Sbjct: 360 IQQLLQSQVIRESSSPYASPIVLVQKKDGGLRMCVDYRQLNARTRKDAFPLPRIDESLDALAGAQWFTTLDLASGYSQVEVAEKDKAKTAFCTPFGLFEFNRMPFGLCNAPSTFQRLMERLFGDCRFQSVLLYLDDVIIFSSSVEQHLQRLEQVFSRLEAQGLKVKLSKCHFFQKQVKYLGHVVSAEGVSTDP 552 HSP 2 Score: 40.817 bits (94), Expect = 1.208e-46 Identity = 42/154 (27.27%), Postives = 67/154 (43.51%), Query Frame = 1 Query: 1513 ESFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHV-KGICYSLWFET------------YDSV*KRLLYNEEKPSRCT*ICYEF*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSR 1935 +SF LK L AP+L Y + F+LE AS G +G+V S +G + F + Y S+ L+ + + +F L + TD+ + T K + Q + LA +D +KY+ G +++NAD LSR Sbjct: 625 KSFQELKTALITAPVLAYANFQKPFVLEVDASHGGLGAVLSQEHEGRRRPVAFASRSLKPTERNMNNYSSMKLELVALKWA------VTEKFREYLLGNACTVFTDNNPLSHLATAK--LGATEQRWASELAAFDLTIKYRPGSQNANADALSR 770 HSP 3 Score: 35.8094 bits (81), Expect = 1.208e-46 Identity = 15/31 (48.39%), Postives = 18/31 (58.06%), Query Frame = 3 Query: 1392 PKCSKQLRSFLRLTNYYRRFIKDYGRIAEPF 1484 P LRSFL +YYRRFI + +IA P Sbjct: 564 PANLADLRSFLGFASYYRRFIAGFAKIASPL 594
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Medaka
Match: ENSORLT00000040501.1 (pep primary_assembly:ASM223467v1:1:1251188:1255180:-1 gene:ENSORLG00000023024.1 transcript:ENSORLT00000040501.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 149.828 bits (377), Expect = 2.449e-46 Identity = 75/193 (38.86%), Postives = 118/193 (61.14%), Query Frame = 1 Query: 784 IKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNS 1362 I++L++++VI+ S + + +V+V KKDG +RMCVDYR LNA T K++F LD L A F+++DL Y QVE+A + +TAF G F FNR+ L AP+TFQ LM + + + V++YLDD++I+ S + H + LE++F R+ GLK+ KC F + ++ +LGH + E + T+ Sbjct: 360 IQQLLQSQVIRESSSPYASPIVLVQKKDGGLRMCVDYRQLNARTRKDAFPSPRIDESLDALAGAQWFTTLDLASGYSQVEVAEKDKAKTAFCTPFGLFEFNRMPFGLCNAPSTFQRLMERLFGDCRFQSVLLYLDDVIIFSSSVEQHLQRLEQVFSRLDAQGLKVKLSKCHFFQKQVKYLGHVVSAEGVSTDP 552 HSP 2 Score: 42.3578 bits (98), Expect = 2.449e-46 Identity = 43/154 (27.92%), Postives = 67/154 (43.51%), Query Frame = 1 Query: 1513 ESFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHV-KGICYSLWFET------------YDSV*KRLLYNEEKPSRCT*ICYEF*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSR 1935 ESF LK L AP+L Y + F+LE AS G +G+V S +G + F + Y S+ L+ + + +F L + TD+ + T K + Q + LA +D +KY+ G +++NAD LSR Sbjct: 625 ESFQELKTALITAPVLAYADFQKPFVLEVDASHGGLGAVLSQEHEGKRRPVAFASRSLKPTERNMNNYSSMKLELVALKWA------VTEKFREYLLGNACTVFTDNNPLSHLATAK--LGATEQRWASELAAFDLTIKYRPGSQNANADALSR 770 HSP 3 Score: 35.4242 bits (80), Expect = 2.449e-46 Identity = 15/31 (48.39%), Postives = 18/31 (58.06%), Query Frame = 3 Query: 1392 PKCSKQLRSFLRLTNYYRRFIKDYGRIAEPF 1484 P LRSFL +YYRRFI + +IA P Sbjct: 564 PANLADLRSFLGFASYYRRFIAGFAKIASPL 594
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Medaka
Match: ENSORLT00000036827.1 (pep primary_assembly:ASM223467v1:16:28613823:28617539:1 gene:ENSORLG00000023550.1 transcript:ENSORLT00000036827.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 172.555 bits (436), Expect = 2.358e-44 Identity = 141/459 (30.72%), Postives = 213/459 (46.41%), Query Frame = 1 Query: 775 EDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGS-IRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNSXXXXXXXXXXXXXXXS*DHFSD*RTTTED--SSKTMAESQNRFMSQ---------HLYVTN----------ESFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHVKGI---CYSLWFETYDSV*KRLLYNEEKPSRCT*ICYEF*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSR----LKG-MICTKRQ------TRHEESKEGKSNI---RYVYSIKRTE 2034 + I+++++ +I+ S W + +V+V KK G +R CVDYR LN +T K+S+ + LD ++ + FSS+DL Y+QV L+P + +TAF G + F L L AP TF+ LM VL + + +VYLDDIL++ S K E L ++ +RI AGLK++P+KC F + EL FLGH E I T + + + TT D S +A RF+ HL + ++F LK L +PIL P FIL+T AS +G+V S + + + +T +R + F F +RTDH A ++ T K P Q L LA + F ++++ G H+NAD +SR L G C KR+ +R E+S G+S+ R V + TE Sbjct: 289 DSAIEEMLRGGIIEPSDSPWASGVVMVKKKKGPKMRFCVDYRPLNGVTKKDSYPLPRIDESLDLVSGSSWFSSLDLRSGYWQVPLSPAARPKTAFCTGRGLWQFRVLSFGLCNAPATFERLMEKVLASIPRQECLVYLDDILVHGGSFKAALESLRKVLQRIAAAGLKLHPDKCCFMRRELEFLGHKIGGEGISTLEEKVQAVRDWPTP------------TTLRDLKSFLGLASYYRRFVRGFSCIAAPLFHLQRKDCDFVWTQECEQAFSSLKKALTNSPILTPPDPKLPFILDTDASDVGMGAVLSQMGSAGERVVAYFSKTLSKAERRYCVTRRELLAVVKAIGHFRYYLCGLPFTVRTDHSALQWLMTFKEPE-GQIARWLEELASFSFTVEHRPGSRHANADAMSRRPCALAGCQYCEKREAREAVISREEQSHTGESSWPVCRLVQGVDSTE 734
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Medaka
Match: ENSORLT00000033871.1 (pep primary_assembly:ASM223467v1:5:24767545:24770648:1 gene:ENSORLG00000024878.1 transcript:ENSORLT00000033871.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 139.428 bits (350), Expect = 3.623e-44 Identity = 67/193 (34.72%), Postives = 127/193 (65.80%), Query Frame = 1 Query: 784 IKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNS 1362 +++L A VI+ +SS+++ +V+V KK+G +R+C+DYR LN T+K+++++ N + LT + FS +DL YYQ+E+ +++TAF G + FNR+ ++ AP+TFQ LM + ++ LK V+V+LDD++++ ++ ++H+ L + R++ GLK++ EKC+F +T + +LGH + ++T+ Sbjct: 206 LQELSHAGVIRESESSFSSPIVVVRKKNGDVRLCIDYRKLNMQTIKDAYALPNLEETFSALTGSKWFSVLDLKSGYYQIEVDEVDKQKTAFVCPLGFWEFNRMPQGITNAPSTFQRLMEKCMGDINLKEVLVFLDDLIVFSETLEEHEARLLNVLNRLRAYGLKLSLEKCKFFQTSVRYLGHIVSQHGVETDP 398 HSP 2 Score: 45.8246 bits (107), Expect = 3.623e-44 Identity = 21/45 (46.67%), Postives = 29/45 (64.44%), Query Frame = 3 Query: 1362 QVEEIEKAEEPKCSKQLRSFLRLTNYYRRFIKDYGRIAEPFYEST 1496 +VE ++ PK K+LRSFL YYRRFI+DY +I +P + T Sbjct: 400 KVEALKTWPVPKNLKELRSFLGFAGYYRRFIRDYSKIVKPLNDLT 444 HSP 3 Score: 34.6538 bits (78), Expect = 3.623e-44 Identity = 34/145 (23.45%), Postives = 64/145 (44.14%), Query Frame = 1 Query: 1513 ESFIVLKNRLCVAPILDYPREDRTFILETYAS-FGIIGSVWSHVKGICYSLWFETYDSV*KRLLYNEEKPSRCT---*ICYEF*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSR 1935 E+F + +L APIL + ++L T AS G+ +++ +G + F + Y K + +F F + TD ++ TT ++ L+ L+ + F+L+Y+ G+++ +ADGLSR Sbjct: 480 EAFETIIEKLTTAPILAFANPKLPYVLHTDASTIGLGAALYQEQEGELRVIAFASRGLSRSEARYPAHKLEFLALKWAVTEKFCDYLYGSFFTVVTDSNPLTYILTTAKLDATSYRW-LSALSTFSFQLQYRAGKQNMDADGLSR 623
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Planmine SMEST
Match: SMESG000081257.1 (SMESG000081257.1) HSP 1 Score: 358.221 bits (918), Expect = 7.177e-110 Identity = 202/458 (44.10%), Postives = 275/458 (60.04%), Query Frame = 1 Query: 736 NIHGVNQCTEKRMEDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNSXXXXXXXXXXXXXXXS*DHFSD*RTTTE-DSSKTMAESQNRFMSQH------LYVTN--------------ESFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHVKGICYSLWFETYDSV*KRLLYNEEKPSRCT*ICYEF*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSRLKGMICTKRQTRHEESKEGKSNIRYVYSIKRTETMEK 2046 N G E ++E+L++ L++A V+++C+S WN LVIVGK DGS+R+C+D+R LN++T K SF M + + LLDCL + IF SIDL QAYY VEL + Q +TAFS +EGQFCFNRL LSTAP TFQ LMH +LE ++ KGVVVYLDDILIY ++++ HD+LL E+F RI+ +GLK+NPEKC FNK+ L F+GHT + + IQTN + ++ ++ ++TTE S + RF+ + LY + FI LK LC APIL+YPR DR FI++T SF IG + H G C + K LL E F + ++F+ RTDH A FMNTTK PI PQFQT +AN +EYDF L+Y+KG EH NA G SRL IC+ Q H+++K+ K R + S++ + + K Sbjct: 456 NPRGQPMHLEAKVEELVQNLLEAGVVRKCQSPWNTPLVIVGKPDGSVRICLDFRLLNSVTEKFSFPMPDMQLLLDCLGKSKIFYSIDLGQAYYLVELNENSQIKTAFSTKEGQFCFNRLPFGLSTAPATFQKLMHQILEGLVFKGVVVYLDDILIYGENQETHDKLLFEVFTRIRDSGLKVNPEKCAFNKSVLNFIGHTVSEKGIQTNKRKISEI-----------ENATEPKSTTELRSFLGLTTYYRRFIKNYSMIAAPLYAATTGCDKMIVWTEECRKRFINLKKLLCEAPILEYPRADRLFIIDTDDSFEAIGHLTKHEIGYCVTR---------KELLVLHE-------FIVHFRQYLYGRRFVARTDHKALTFMNTTKKPISPQFQTWMANFSEYDFALQYRKGNEHGNAGGWSRLNNTICSHYQMEHKDAKKAKCRTRCINSLQGSSNIMK 886 HSP 2 Score: 70.4774 bits (171), Expect = 3.539e-12 Identity = 32/54 (59.26%), Postives = 40/54 (74.07%), Query Frame = 3 Query: 1350 SNK*QVEEIEKAEEPKCSKQLRSFLRLTNYYRRFIKDYGRIAEPFYESTSVCDK 1511 +NK ++ EIE A EPK + +LRSFL LT YYRRFIK+Y IA P Y +T+ CDK Sbjct: 662 TNKRKISEIENATEPKSTTELRSFLGLTTYYRRFIKNYSMIAAPLYAATTGCDK 715 HSP 3 Score: 66.2402 bits (160), Expect = 6.541e-11 Identity = 39/106 (36.79%), Postives = 62/106 (58.49%), Query Frame = 1 Query: 397 MYQTKDGRIWGKIILNSTEVDMLWDSGTSVTVMSKNLWEVIGKPDLQRSAILLCGVFST------GEESMVCMXXXXXXXXXXXXXXXXXXXFIGGIDIIEKFGVK 696 M++ D R W + +N V+MLW+SG S++VMS+ W +IG P L S ILL VFS G +V + + V+++++I P FIGG+D ++ FG++ Sbjct: 276 MFKKDDDRKWSIMEINGFHVEMLWNSGASISVMSEKCWRLIGSPILMDSRILLSEVFSNEDKKPLGSVKIVAKWNKKFRELNVIIVRKIHPNFIGGVDTMKIFGME 381 HSP 4 Score: 50.8322 bits (120), Expect = 3.400e-6 Identity = 20/37 (54.05%), Postives = 25/37 (67.57%), Query Frame = 2 Query: 2 KLTVGDCFCCGERLHKRYKCPLKN*CLKPGNEDHGFR 112 +L G CFCCG+ H R++CPLK+ CL G E H FR Sbjct: 180 RLKPGKCFCCGQNDHMRFECPLKDKCLICGKEGHSFR 216
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Planmine SMEST
Match: SMESG000038955.1 (SMESG000038955.1) HSP 1 Score: 354.755 bits (909), Expect = 1.007e-109 Identity = 203/437 (46.45%), Postives = 265/437 (60.64%), Query Frame = 1 Query: 763 EKRMEDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNSXXXXXXXXXXXXXXXS*DHFSD*RTTTEDSSKTMAESQNRFMSQHLYVTNESFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHVKGICYSLWFETYDSV*KRLLYNEEKPSRCT*I---------CYEF*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSRLKGMICTKRQTRHEESKEGKSNIRYVYSIKRTETMEK 2046 E ++E+L++ L++A V+++ S WN LVIVGK DGSIRMC+D+R LN++T K SF + K LLDCL ++ IFSSIDL QAYYQVEL + Q +TAFS +EGQFCFNRL LSTAP TFQ LMH +LE ++ KGVVVYLD+ILIY ++++ HD+LL E+F RI+ +GLK+NPEKC FNK+ L F+GHT N I + TT D E SFI LK LC APIL+YPR DR F+++T ASFG IG+V S +K C + Y S R L E C I F + ++F+ RTDH A FMNTTK PI PQFQT +ANL+E+DF L+Y+KGEEH NADG SRL C+ ++++KE K RY+ S++ + + K Sbjct: 154 EGKVEELVQNLLEAGVVRKSISPWNTPLVIVGKLDGSIRMCLDFRLLNSVTEKFSFYSPDMKLLLDCLGNSKIFSSIDLGQAYYQVELNENSQIKTAFSTKEGQFCFNRLPFGLSTAPATFQKLMHQILEGLVFKGVVVYLDEILIYGENQEIHDKLLFEVFTRIRDSGLKVNPEKCAFNKSVLNFIGHT-NYSIIAAPMYVA---------------------TTGNDKMIVWTEE-----------CRNSFINLKKLLCEAPILEYPRADRLFVIDTDASFGAIGAVLSQIKEDCTEVVI-AYGS---RHLTKHEM-GHCVTIKELLALHEFIVHFRQYLYGRRFVARTDHKALTFMNTTKKPISPQFQTWMANLSEHDFALQYRKGEEHGNADGKSRLNNTKCSHCLMENKDAKEAKCRTRYINSLQGSSNIMK 552 HSP 2 Score: 58.5362 bits (140), Expect = 1.422e-8 Identity = 26/48 (54.17%), Postives = 32/48 (66.67%), Query Frame = 1 Query: 439 LNSTEVDMLWDSGTSVTVMSKNLWEVIGKPDLQRSAILLCGVFSTGEE 582 +N VDMLWDSG S++VMS W +IG+P L S I L GVFS +E Sbjct: 3 INGFHVDMLWDSGASISVMSDKYWRLIGRPILMDSRIRLSGVFSKEDE 50
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Planmine SMEST
Match: SMESG000065302.1 (SMESG000065302.1) HSP 1 Score: 272.322 bits (695), Expect = 1.711e-91 Identity = 165/386 (42.75%), Postives = 227/386 (58.81%), Query Frame = 1 Query: 322 HFVGGVKTMNCGLRNERRTEKAEKEMYQTKDGRIWGKIILNSTEVDMLWDSGTSVTVMSKNLWEVIGKPDLQRSAILLCGVFST------GEESMVCMXXXXXXXXXXXXXXXXXXXFIGGIDIIEKFGVKY--------------------------------DTRCGRQEVQ*CNIHGVNQCTEKRMEDL--IKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTN 1359 HFV VKT++ +R + EM++ D R W + +N ++MLWDSG S++VMS+ W +IG P L S I L GVFS G +V + V+++++I P+FIGG+D ++ F +K +++ G Q NI ++ DL IK L+ A V+++C+SSWN LVIVGK DGS RMC+D+R LN++T K SF M + + LLDCL + I SSIDL Q YYQVEL + Q +TAFS +EGQFCFNRL LSTAP TFQ LMH +LE ++ K VVVYLDDIL Y ++++ HD+LL E+F RI +GLK++P KC FNK+ L F+ HT + + IQTN Sbjct: 14 HFVSKVKTVD---EFNKRNVGKKNEMFKKDDDRKWSMMEINGFHLEMLWDSGASISVMSEKCWRLIGSPILMDSRIRLSGVFSKEYEKPFGSVKIVAKRNKKVREFNVIIVRKIHPDFIGGVDTMKIFCMKLKEVNIIESSLVNKRFTDSDRLKNTLLTLKLDKNSKLGTLISQFSNIFMASKF------DLGHIKNLLVAGVVRKCQSSWNTPLVIVGKPDGSSRMCLDFRCLNSVTEKVSFPMPDMQLLLDCLGKSKILSSIDLRQVYYQVELNENSQIKTAFSTKEGQFCFNRLPFGLSTAPATFQKLMHQILEGLVFKRVVVYLDDILKYAENQETHDKLLFEVFTRIIDSGLKVSPAKCAFNKSLLNFINHTVSEKGIQTN 390 HSP 2 Score: 59.6918 bits (143), Expect = 1.711e-91 Identity = 34/65 (52.31%), Postives = 41/65 (63.08%), Query Frame = 3 Query: 1350 SNK*QVEEIEKAEEPKCSKQLRSFLRLTNYYRRFIKDYGRIAEPFYESTSVCDK*KFHSTEEQAV 1544 +NK + EI E K + +LRSFL LTNYYRRFIK+Y IA P Y +T+ DK TEE AV Sbjct: 389 TNKGKFSEIVNPIETKSTTELRSFLGLTNYYRRFIKNYSMIAAPLYAATTGNDK-MIVWTEECAV 452 HSP 3 Score: 46.595 bits (109), Expect = 1.711e-91 Identity = 19/39 (48.72%), Postives = 29/39 (74.36%), Query Frame = 2 Query: 1646 ESVTAYGLRHMTAYEKGYCIMRKNLLAVHEYVMNFREYF 1762 E V AY RH+T +E GYC++RK LL ++E +++FR+Y Sbjct: 462 EGVIAYVSRHLTNHEMGYCVIRKELLVLYELIVHFRQYL 500 HSP 4 Score: 51.6026 bits (122), Expect = 1.932e-6 Identity = 23/50 (46.00%), Postives = 32/50 (64.00%), Query Frame = 1 Query: 1897 KGEEHSNADGLSRLKGMICTKRQTRHEESKEGKSNIRYVYSIKRTETMEK 2046 KGEEH N+DG+SRL IC+ Q ++SKEGK RY+ S+ + + K Sbjct: 530 KGEEHGNSDGISRLNNTICSHCQIEQKDSKEGKCRTRYINSLHGSSNIMK 579
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Planmine SMEST
Match: SMESG000029259.1 (SMESG000029259.1) HSP 1 Score: 258.84 bits (660), Expect = 4.699e-73 Identity = 164/430 (38.14%), Postives = 245/430 (56.98%), Query Frame = 1 Query: 763 EKRMEDLIKKLIKAKVIQRCKSSWNARLVIVGKKDG-SIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQT-NSXXXXXXXXXXXXXXXS*DHF----SD*RTTTEDSSKTMAE-----SQNRFMSQHLYVTNESFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHV--KGICYSLWFETY--DSV*KRLLYNEEKPSRCT*ICYEF*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQT*LANLAEYDFELKYKKGEEHSNADGLSRLKGMICTKRQTRHEESKEGKSNIR 2007 E ++E+ IK L +I++C S WN LV V KK+ IR+C+D+R LN IT +++F M N +LD L + FSSIDL AYYQV+L + QE+TAFS +EGQFCFNR+ ++ AP TFQ LM VL+++ GV+VYLDDILI+ K+++DH + ++ +I AGL++NPEKC+ + E+ FLGH N + IQT N+K++ ++ + + F + R +D +K +N + + ++F +K L AP+L +P + FIL+T ASF IG+V S KG + + + ++ S K ++ C F K+F++RTDH A FM TTK PI QFQT + L+ D +++Y+KG H+NAD LSR C + HE++K GK R Sbjct: 2740 EDKIEEAIKNLENNGIIRKCNSPWNTPLVCVWKKEKKDIRLCLDFRQLNKITERQAFPMPNVDEILDILHGSRYFSSIDLGNAYYQVKLDKESQEKTAFSTKEGQFCFNRMPFGIAAAPGTFQELMTKVLKDLWKDGVMVYLDDILIFTKTEEDHYNIFGKVLGKIATAGLRLNPEKCQIFRKEVKFLGHIINKDGIQTDNTKIEAIQSFQKPKCVKNLRSFLGICNYYRRFIKDYAKKARALESICGKNNEKIRWTEMCEKAFGEMKEALITAPVLVFPDFRKEFILDTDASFDTIGAVLSQKDEKGHEHVIAYGSHAMSSHEKGYCITRKELLAIYYFCKHFNHYLYGKRFVLRTDHKAITFMVTTKKPITAQFQTWINYLSSLDIKMEYRKGTSHTNADMLSRKTCGTCVQCMMEHEDAKTGKIKTR 3169 HSP 2 Score: 52.7582 bits (125), Expect = 1.266e-6 Identity = 25/50 (50.00%), Postives = 33/50 (66.00%), Query Frame = 3 Query: 1362 QVEEIEKAEEPKCSKQLRSFLRLTNYYRRFIKDYGRIAEPFYESTSVCDK 1511 ++E I+ ++PKC K LRSFL + NYYRRFIKDY + A S+C K Sbjct: 2942 KIEAIQSFQKPKCVKNLRSFLGICNYYRRFIKDYAKKARAL---ESICGK 2988
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Planmine SMEST
Match: SMESG000035021.1 (SMESG000035021.1) HSP 1 Score: 224.557 bits (571), Expect = 2.635e-65 Identity = 177/528 (33.52%), Postives = 261/528 (49.43%), Query Frame = 1 Query: 412 DGRIWGKIILNSTEVDMLWDSGTSVTVMSKNLWEVIGKPDLQRSAILLCGVFSTGEESM-----VCMXXXXXXXXXXXXXXXXXXXFIGGIDIIEKFGVKY--------------------------------DTRCGRQEVQ*CNIHGVNQCTEKRMEDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKESFSMSNTKFLLDCLTDAIIFSSIDLDQAYYQVELAPDIQERTAFSIREGQFCFNRLLVRLSTAPTTFQ*LMHTVLEEMLLKGVVVYLDDILIYRKSKKDHDELLEELFKRIQMAGLKINPEKCEFNKTELCFLGHTFNCESIQTNSXXXXXXXXXXXXXXXS*DHFSD*RTTTE-DSSKTMAESQNRFMSQHLYVTNESFIVLKNRLCVAPILDYPREDRTFILETYASFGIIGSVWSHVKG------ICYSLWFET-----YDSV*KRLLYNEEKPSRCT*ICYEF*RIFLRKKFIIRTDHYAFVFMNTTKTPI*PQFQ 1848 DGR W + LN V+MLWDSG S++V+S+ W +IG P L S I L GVF +E + V + + V+++++I P+FIGG+D I+ FG++ +T+ G Q NI ++ ++ +I IK ++S + + GK + ++ L A L++S S NT ++ D ++ QAYYQVEL + Q +TAFS +EGQ CFNRL + LSTAP TFQ ++ L+ ++ KG V+YLDDILIY ++++ +D+LL E+F RI+ +GLK NPEKC FNK+ L F+GHT + + IQTN + ++ ++ ++TTE S + RF+ + + + APIL+YPR DR FI++T SFG IG+V S +K I Y T Y K L E F + K+F+ R DH A FMNTTK PI QF Sbjct: 4 DGRKWSMMRLNGFHVEMLWDSGASISVISEKCWRLIGSPILMDSRIRLSGVFPKEDEKLLGSKIVAKWNKKFRKLNVIIVRKIYPDFIGGVDTIKIFGMELKEVNNIESLLVNKRFTDSELLKITLLTLKFDKNTKLGTLIFQFSNIFMASRFDFGHIK-VIAHEIKTSDSPILQNSRRQLMHLEGKVEELVQNL-----LEAGVLRKSQSPWNTPLVIVGKPDDS--KNVSRFQAYYQVELNENSQIKTAFSTKEGQLCFNRLPIGLSTAPATFQKHINQTLKGLVFKGEVLYLDDILIYAENQETYDKLLFEVFIRIRDSGLKANPEKCAFNKSVLNFIGHTVSEKGIQTNKRKISEI-----------ENATEPKSTTELRSFLGLTNFYQRFIKNYSMIAEPLYTA-----TTAPILEYPRADRLFIIDTDDSFGAIGAVLSQIKEDGTEGVIAYGSRHLTEHEMEYCMTRKELFALHE-------FIVHFRQYLYGKRFVARMDHKALTFMNTTKKPISSQFH 500 HSP 2 Score: 56.9954 bits (136), Expect = 3.191e-8 Identity = 27/57 (47.37%), Postives = 36/57 (63.16%), Query Frame = 2 Query: 1622 GRFGATLK-------ESVTAYGLRHMTAYEKGYCIMRKNLLAVHEYVMNFREYFYGK 1771 G GA L E V AYG RH+T +E YC+ RK L A+HE++++FR+Y YGK Sbjct: 418 GAIGAVLSQIKEDGTEGVIAYGSRHLTEHEMEYCMTRKELFALHEFIVHFRQYLYGK 474 The following BLAST results are available for this feature:
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30015933 ID=SMED30015933|Name=Transposon Ty3-I Gag-Pol polyprotein|organism=Schmidtea mediterranea sexual|type=transcript|length=2084bpback to top protein sequence of SMED30015933-orf-1 >SMED30015933-orf-1 ID=SMED30015933-orf-1|Name=SMED30015933-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=129bp MNCGLRNERRTEKAEKEMYQTKDGRIWGKIILNSTEVDMLWDSGTSVTVMback to top protein sequence of SMED30015933-orf-2 >SMED30015933-orf-2 ID=SMED30015933-orf-2|Name=SMED30015933-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=120bp MEDLIKKLIKAKVIQRCKSSWNARLVIVGKKDGSIRMCVDYRGLNAITLKback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|