SMED30015908

Overview
NameSMED30015908
Smed IDSMED30015908
Length (bp)867
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30015908 (SMED30015908) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30015908 (SMED30015908) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30015908 (SMED30015908) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30015908 (SMED30015908) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30015908

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30015908h1SMcG0012248 dd_Smed_v4_8749_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30015908h1SMcG0012248 dd_Smed_v4_8749_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30015908h1SMcG0012248 dd_Smed_v4_8749_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30015908h1SMcG0012248 dd_Smed_v4_8749_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30015908h1SMcG0012248 dd_Smed_v4_8749_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30015908 vs. Planmine SMEST
Match: SMESG000056674.1 (SMESG000056674.1)

HSP 1 Score: 202.216 bits (513), Expect = 4.507e-66
Identity = 99/102 (97.06%), Postives = 99/102 (97.06%), Query Frame = 2
Query:  101 MYKTIHYDKCELDVIDPLTISTDFEGEAENKQISVKMFNKLNVVWLKWRGRVNGLLCIGCELKNKVECGTNGKFGSQKLFDCSEKRGVLIVINDNISTCVNA 406
            M KTIHYDKCELDVIDPLTI TDFEGEAENKQISVKMFNKLNVVWLKWRGRVNGLLCIGCELKNKVECGTNGKFGSQKLFDCSEKRGVLIVINDNISTCVN 
Sbjct:    1 MSKTIHYDKCELDVIDPLTIGTDFEGEAENKQISVKMFNKLNVVWLKWRGRVNGLLCIGCELKNKVECGTNGKFGSQKLFDCSEKRGVLIVINDNISTCVNG 102          
BLAST of SMED30015908 vs. Planmine SMEST
Match: SMESG000056668.1 (SMESG000056668.1)

HSP 1 Score: 173.711 bits (439), Expect = 3.466e-55
Identity = 85/85 (100.00%), Postives = 85/85 (100.00%), Query Frame = 2
Query:  470 MSLNELSKHAYFKIGVKICKTLKSESKCGTCKWVGLVNGVECIGAEMDSPQKNGTAGSFNGFNLFNCQGKHGVIYKADGVSVVKN 724
            MSLNELSKHAYFKIGVKICKTLKSESKCGTCKWVGLVNGVECIGAEMDSPQKNGTAGSFNGFNLFNCQGKHGVIYKADGVSVVKN
Sbjct:    1 MSLNELSKHAYFKIGVKICKTLKSESKCGTCKWVGLVNGVECIGAEMDSPQKNGTAGSFNGFNLFNCQGKHGVIYKADGVSVVKN 85          
BLAST of SMED30015908 vs. Planmine SMEST
Match: SMESG000007087.1 (SMESG000007087.1)

HSP 1 Score: 127.102 bits (318), Expect = 1.444e-32
Identity = 77/209 (36.84%), Postives = 112/209 (53.59%), Query Frame = 2
Query:  116 HYDKCELDVIDPLTISTDFEGEAENKQISVKMFNKLNVVWLKWRGRVNGLLCIGCELKNKVECGTNGKFGSQKLFDCSEKRGVLIVINDNISTCVNARHWEIAVC-------RHDT--VEKNSICCTMSLNELSKHAYFKIGVKICKTLKSESKC--GTCKWVGLVNGVECIGAEMDSPQKNGTAGSFNGFNLFNCQGKHGVIYKADGV 709
            H  +  L+V+DP  +     G   NK    K    + ++ +KWRG VNG LC+GCEL   VE GT+G FG++ +F C  +RGVLI   + I +C    H +I          + DT  +  + ICCT S   L KH   +I  +I K    E+K   G+C+W+GL+NG E +G  MD+  K GT G F+ F +F C+   GV+  A+ +
Sbjct:  948 HVKEINLNVVDPFNVREIENGRQINKLTCYKSKGAIKILNIKWRGIVNGELCVGCELSGPVEWGTDGTFGNKIVFKCPARRGVLIPTEELIDSCETTAHSKITTLPNSGWLWKGDTYFINPDCICCTESTESLQKHPDLRIKSRI-KVPNKENKQIGGSCEWIGLINGQEALGITMDNDVK-GTNGIFSCFRMFTCESGKGVLMLAENI 1154          

HSP 2 Score: 106.301 bits (264), Expect = 1.975e-25
Identity = 74/198 (37.37%), Postives = 107/198 (54.04%), Query Frame = 2
Query:   98 TMYKTIHYDKCELDVIDPLTIST--DFEGEAENKQISVKMFNKLNVVWLKWRGRVNGLLCIGCELKNKVECGTNGKFGSQKLFDCSEKRGVLIVINDNISTCVNARHW-EIAVCRHDTVEKNSICCTMSLNELSKHAYFKIGVKICKTLKSESKCGTCKWVGLVNGVECIGAEMDSPQKNGTAGSFNGFN-LFNCQGK 679
            ++  TI  DK  LDVI P  I      +G   NK I+++   K + V L+W+G++N + C+GCEL      GT G FG  KLF CS  RGVLI+IND + +C +A     + +  +D +  N+ICC   L++LSK       V  C+ LK+    G CKW+GL+NG   +G E D   +   A  FN  + + N  G+
Sbjct:   39 SVLPTICCDKLGLDVIKPGAIDQIGKLKGAQMNKYIAIQGKTK-SFVKLQWKGKINNINCLGCELPEFWSEGTEGTFGRYKLFTCSYGRGVLILINDAVKSCESAEESNHLPLADNDELTANNICCFEPLDKLSKDIKVNSQV-FCQQLKA---GGKCKWMGLINGKGYVGIEFDEIHRR--AERFNELSRILNIDGR 229          

HSP 3 Score: 98.9821 bits (245), Expect = 7.686e-23
Identity = 71/192 (36.98%), Postives = 95/192 (49.48%), Query Frame = 2
Query:  134 LDVIDPLTISTDFEGEA--ENKQISVKMFNKLNVVWLKWRGRVNGLLCIGCELKNKVECGTNGKFGSQKLFDCSEKRGVLIVINDNISTCVNARHWEIAVCRHDTVEKNSICCTMSLNELSKHAYFKIGVKICKTLKSESKCGTCKWVGLVNGVECIGAEMDSPQKNGTAGSFNGFNLFNCQGKHGVIYKAD 703
            + VIDP  I    EG A    K I     N+ + V LKW+G ++   C+GC    +   GTNG FG  KLF C +K G+LI +      C    H  I   R   +++NSICCT+  N     A  K+     K +KS  K    KW GLVN VE IG EM+   + GT G+F    LF    K G ++K++
Sbjct:  753 IPVIDPEFIRI-IEGVAVEPGKFIVFTCNNQRHSVQLKWKGLIDQKYCLGCVANFQCNFGTNGTFGQDKLFACEDKHGILIPLAAVSECCEMKGHKPIKWTRDGAIDENSICCTVEKNITKFQANCKL-----KNIKSNKK-AIWKWSGLVNDVEVIGVEMNESLEKGTDGNFCKIPLFRSVTKRGALFKSE 937          

HSP 4 Score: 66.6254 bits (161), Expect = 4.612e-12
Identity = 49/160 (30.63%), Postives = 71/160 (44.38%), Query Frame = 2
Query:  227 VVWLKWRGRVNGLLCIGCELKNKVECGTNGKFGSQKLFDCSEKRGVLIVINDNISTCVNARHW-EIAVCRHDTVEKNSICCTMSLNELSKHAYFKIGVKICKTLKSESKCGTCKWVGLVNGVECIGAEMDSPQKNGTAGSFNGFNLFNCQGKHGVIYKAD 703
             +W KW G VN +  IG E+   +E GT+G F    LF    KRG L    + I++    +H  EI +   D      I     +N+L+           C   K   K    KW G+VNG  C+G E+  P + GT G+F    +F C  + GV+   +
Sbjct:  888 AIW-KWSGLVNDVEVIGVEMNESLEKGTDGNFCKIPLFRSVTKRGALFKSEEFINSKSELKHVKEINLNVVDPFNVREIENGRQINKLT-----------CYKSKGAIKILNIKWRGIVNGELCVGCELSGPVEWGTDGTFGNKIVFKCPARRGVLIPTE 1035          

HSP 5 Score: 58.5362 bits (140), Expect = 2.681e-9
Identity = 44/156 (28.21%), Postives = 71/156 (45.51%), Query Frame = 2
Query:  236 LKWRGRVNGLLCIGCELKNKVECGTNGKFGSQKLFDCSEKRGVLIVI-NDNISTCVNARH-WEIAVCRHDTVEKNSICCTMSLNE--LSKHAYFKIGVKICKTLKSESKC-GTCKWVGLVNGVECIGAEMDSPQKNGTAGSFNGFNLFNCQGKHGV 688
            LKW G++N   C+G +++++ E G +G       FDC EK G++ V+ N N+    N  H     +   D   +    C  +  +  +      K G+   K L  ++   GT KW GL+N V     ++    +NGT G F    LF C+ K  +
Sbjct:  290 LKWIGKINNEKCVGLDMEDETEFGMDGIINQDNRFDCMEKHGLITVLSNVNLYKYDNNVHLLPSGIINPDKYLR----CKETFGDECIKAQRIKKDGMVNVKLLNGQANLKGTLKWHGLMNNVPVCAVQLLQEHENGTNGRFWRTRLFECRDKLAI 441          

HSP 6 Score: 52.373 bits (124), Expect = 2.204e-7
Identity = 45/160 (28.12%), Postives = 66/160 (41.25%), Query Frame = 2
Query:  236 LKWRGRVNGLLCIGCELKNKVECGTNGKFGSQKLFDCSEKRGVLIVINDNISTCVNARHWEIAVCRHDTVEKNSICCTMSLNELSKHAYFKIGVKICKTLKSESKCGTCKWVGL-VNGVECIGAEMDSPQKNGTAGSFNGFNLFNCQGKHGVIYKADGVS 712
            L+W G +N LL    EL    E G+NG F  +++ +   KR         I+T VN  +           E+  I   + +N   K+ +             +   GT KW G+  N     G E+D     GT G+F G  LF CQ  HGV+   + V+
Sbjct: 1322 LRWIGWMNKLLVCVIELDQDHENGSNGTFWRRRIVNSRGKRV--------IATSVNHVYKYEPYLSKAIPEEIQIFKEIVINSAVKYYFLT-------ATGYQDLFGTVKWTGVDENDCYFFGLELDDNIDIGTDGTFGGQRLFTCQNGHGVLAPLNTVA 1466          

HSP 7 Score: 51.6026 bits (122), Expect = 3.809e-7
Identity = 42/163 (25.77%), Postives = 71/163 (43.56%), Query Frame = 2
Query:  239 KWRGRVNGLLCIGCELKNKVECGTNGKFGSQKLFDCSEKRGVLIVINDNISTCVNARHWEIAVCRHDTVEKNSICCTMSLNELSKHAYFKIGVKICKTLKSESKCGTCKWVGLVNGVECIGAEM-DSPQKNGTAGSFNGFNLFNCQGKHGVIYKADGVSVVKN 724
            +W G +NG   +G  + N V+ GTNG F   ++F C   +GVL++  +        +   IA    D  +  SI      N +      KI +      ++    G  KW G +N  +C+G +M +   ++G  G      +F C  KHG++     V + +N
Sbjct: 1107 EWIGLINGQEALGITMDNDVK-GTNGIFSCFRMFTCESGKGVLMLAENIFHDKEREKPQFIADKIVDINKIYSISPCEGENGIGIGKPIKINLN-SPNGRNRDFIGEVKWTGRINDEKCVGLDMVEEEMEHGMDGMIGKSRIFQCMEKHGLVALLSDVELSEN 1267          
BLAST of SMED30015908 vs. Planmine SMEST
Match: SMESG000074227.1 (SMESG000074227.1)

HSP 1 Score: 126.716 bits (317), Expect = 1.980e-32
Identity = 73/202 (36.14%), Postives = 111/202 (54.95%), Query Frame = 2
Query:  134 LDVIDPLTISTDFEGEAENKQISVKMFNKLNVVWLKWRGRVNGLLCIGCELKNKVECGTNGKFGSQKLFDCSEKRGVLIVINDNISTCVNARHWEIAVC------RHDT---VEKNSICCTMSLNELSKHAYFKIGVKI-CKTLKSESKCGTCKWVGLVNGVECIGAEMDSPQKNGTAGSFNGFNLFNCQGKHGVIYKADGV 709
            L+V+DP  +     G   NK    K+   + ++ +KWRG V+G LC+GCEL   VECGT+GKFG++ LF   ++RGVLI   +   +C +  H EIA+       R +T   ++ + ICC      L +H   +I  +I    ++++   G C+W+GL+NG E +G  MD   K GT G F+ F +F C    GV+  AD +
Sbjct:  126 LNVVDPFNVREIENGRQINKSTCYKINGAVKILNIKWRGIVDGELCVGCELSGPVECGTDGKFGNKNLFKFPDRRGVLIPTGELRDSCESTAHSEIAILPGRGWPRKETTYFIDPDCICCIELTELLQQHPDLRIKSRIKIHNIENKQIGGLCEWIGLINGQEALGITMDKDVK-GTNGIFSCFRMFTCDKGKGVLVLADNI 326          

HSP 2 Score: 60.8474 bits (146), Expect = 3.568e-10
Identity = 48/167 (28.74%), Postives = 81/167 (48.50%), Query Frame = 2
Query:  197 ISVKMFN-KLNVV-WLKWRGRVNGLLCIGCELKNKVECGTNGKFGSQKLFDCSEKRGVLIVINDNISTCVNARHWEIAVCRHDTVEKNSICCTMSLNELSKHAYFKIGVKICKTLKSESKCGTCKWVGLVNGVECIGAEMDSPQKNGTAGSFNGFNLFNCQGKHGVI 691
            +SVK+ N + N+   LKW G +N +     EL  + E GTNG+F   +LF+C +K  +   +  N+S   +   + I     + V+  ++    +  +++K   +KI   +        K    KW G+V+G  C+G E+  P + GT G F   NLF    + GV+
Sbjct:   47 VSVKLLNGQANLKGTLKWHGSMNNVPVCAVELLQEHENGTNGRFWRTRLFECRDKLAI-ATLQQNLSPIKSELKF-IKEINLNVVDPFNVREIENGRQINKSTCYKINGAV--------KILNIKWRGIVDGELCVGCELSGPVECGTDGKFGNKNLFKFPDRRGVL 203          

HSP 3 Score: 55.0694 bits (131), Expect = 3.044e-8
Identity = 43/167 (25.75%), Postives = 73/167 (43.71%), Query Frame = 2
Query:  239 KWRGRVNGLLCIGCELKNKVECGTNGKFGSQKLFDCSEKRGVLIVINDNISTCVNARHWEIAVCRHDTVEKNSICCTMSLNELSKHAYFKIGVKICKTLKSESK-----CGTCKWVGLVNGVECIGAEMDSPQKNGTAGSFNGFNLFNCQGKHGVIYKADGVSVVKN 724
            +W G +NG   +G  +   V+ GTNG F   ++F C + +GVL V+ DNI   +     +     +  V+ N       ++         IG  I   L S +       G  KW+G +N  +C+G +M+   ++G  G       F C  +HG++     V + +N
Sbjct:  279 EWIGLINGQEALGITMDKDVK-GTNGIFSCFRMFTCDKGKGVL-VLADNIIHGILKESEKTQFIANKIVDINK---PYRISPCGGDNGISIGKPIKMNLNSPNGRNRDFIGEVKWIGRINDEKCVGLDMEDEMEHGMDGKIGQSQRFECMERHGLVALLSDVELHEN 440          
BLAST of SMED30015908 vs. Planmine SMEST
Match: SMESG000074227.1 (SMESG000074227.1)

HSP 1 Score: 126.331 bits (316), Expect = 2.085e-32
Identity = 73/202 (36.14%), Postives = 111/202 (54.95%), Query Frame = 2
Query:  134 LDVIDPLTISTDFEGEAENKQISVKMFNKLNVVWLKWRGRVNGLLCIGCELKNKVECGTNGKFGSQKLFDCSEKRGVLIVINDNISTCVNARHWEIAVC------RHDT---VEKNSICCTMSLNELSKHAYFKIGVKI-CKTLKSESKCGTCKWVGLVNGVECIGAEMDSPQKNGTAGSFNGFNLFNCQGKHGVIYKADGV 709
            L+V+DP  +     G   NK    K+   + ++ +KWRG V+G LC+GCEL   VECGT+GKFG++ LF   ++RGVLI   +   +C +  H EIA+       R +T   ++ + ICC      L +H   +I  +I    ++++   G C+W+GL+NG E +G  MD   K GT G F+ F +F C    GV+  AD +
Sbjct:  126 LNVVDPFNVREIENGRQINKSTCYKINGAVKILNIKWRGIVDGELCVGCELSGPVECGTDGKFGNKNLFKFPDRRGVLIPTGELRDSCESTAHSEIAILPGRGWPRKETTYFIDPDCICCIELTELLQQHPDLRIKSRIKIHNIENKQIGGLCEWIGLINGQEALGITMDKDVK-GTNGIFSCFRMFTCDKGKGVLVLADNI 326          

HSP 2 Score: 60.8474 bits (146), Expect = 3.842e-10
Identity = 48/167 (28.74%), Postives = 81/167 (48.50%), Query Frame = 2
Query:  197 ISVKMFN-KLNVV-WLKWRGRVNGLLCIGCELKNKVECGTNGKFGSQKLFDCSEKRGVLIVINDNISTCVNARHWEIAVCRHDTVEKNSICCTMSLNELSKHAYFKIGVKICKTLKSESKCGTCKWVGLVNGVECIGAEMDSPQKNGTAGSFNGFNLFNCQGKHGVI 691
            +SVK+ N + N+   LKW G +N +     EL  + E GTNG+F   +LF+C +K  +   +  N+S   +   + I     + V+  ++    +  +++K   +KI   +        K    KW G+V+G  C+G E+  P + GT G F   NLF    + GV+
Sbjct:   47 VSVKLLNGQANLKGTLKWHGSMNNVPVCAVELLQEHENGTNGRFWRTRLFECRDKLAI-ATLQQNLSPIKSELKF-IKEINLNVVDPFNVREIENGRQINKSTCYKINGAV--------KILNIKWRGIVDGELCVGCELSGPVECGTDGKFGNKNLFKFPDRRGVL 203          

HSP 3 Score: 55.0694 bits (131), Expect = 2.836e-8
Identity = 41/162 (25.31%), Postives = 73/162 (45.06%), Query Frame = 2
Query:  239 KWRGRVNGLLCIGCELKNKVECGTNGKFGSQKLFDCSEKRGVLIVINDNISTCVNARHWEIAVCRHDTVEKNSICCTMSLNELSKHAYFKIGVKICKTLKSESKCGTCKWVGLVNGVECIGAEMDSPQKNGTAGSFNGFNLFNCQGKHGVIYKADGVSVVKN 724
            +W G +NG   +G  +   V+ GTNG F   ++F C + +GVL V+ DNI     A+       + + +   SI   + +N  S +             ++    G  KW+G +N  +C+G +M+   ++G  G       F C  +HG++     V + +N
Sbjct:  279 EWIGLINGQEALGITMDKDVK-GTNGIFSCFRMFTCDKGKGVL-VLADNIIHEKKAK-------KRNLLRIKSIGKPIKMNLNSPNG------------RNRDFIGEVKWIGRINDEKCVGLDMEDEMEHGMDGKIGQSQRFECMERHGLVALLSDVELHEN 419          

HSP 4 Score: 49.6766 bits (117), Expect = 1.540e-6
Identity = 34/158 (21.52%), Postives = 75/158 (47.47%), Query Frame = 2
Query:  236 LKWRGRVNGLLCIGCELKNKVECGTNGKFGSQKLFDCSEKRGVLIVINDNISTCVNARHWEIAVCRHDTVEKNSICCTMSLNELSKHAYFKIGVKICKTLKSESKCGTCKWVGLVNGVECIGAEMDSPQKNGTAGSFNGFNLFNCQGKHGVIYKADGV 709
            +KW GR+N   C+G ++++++E G +GK G  + F+C E+ G++ +++D +    NA + +I          N I        ++ + + +   +  K    +      +++G +N +     E+D   +NG+ G F    + + +GK  +    + V
Sbjct:  365 VKWIGRINDEKCVGLDMEDEMEHGMDGKIGQSQRFECMERHGLVALLSD-VELHENAVNPQIL--------DNGI--------IAPNKFIRFQEEHSKRYLDDKNIKMNEFIGWMNKLLVCVIELDQDHENGSNGIFWRRRIVDSRGKRVIATHLNNV 505          
The following BLAST results are available for this feature:
BLAST of SMED30015908 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015908 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015908 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015908 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015908 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015908 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015908 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015908 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015908 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015908 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015908 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015908 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015908 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015908 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000056674.14.507e-6697.06SMESG000056674.1[more]
SMESG000056668.13.466e-55100.00SMESG000056668.1[more]
SMESG000007087.11.444e-3236.84SMESG000007087.1[more]
SMESG000074227.11.980e-3236.14SMESG000074227.1[more]
SMESG000074227.12.085e-3236.14SMESG000074227.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015908 ID=SMED30015908|Name=SMED30015908|organism=Schmidtea mediterranea sexual|type=transcript|length=867bp
GCCTTATTAAAAGAAGTCTTCGAATTTCTAAATTTTACTATTAAAATTAG
GATTTCAGATTGTTTTAATTTTGTTAACAAAGGCATAAACTTGGATGACA
ATGTATAAAACAATACATTATGATAAATGTGAGTTGGACGTGATAGACCC
TCTAACTATCAGTACAGATTTTGAAGGGGAGGCAGAAAATAAGCAAATTT
CCGTTAAAATGTTTAATAAACTTAATGTTGTATGGTTGAAATGGAGAGGA
AGAGTTAACGGATTGTTATGCATTGGATGTGAATTGAAAAATAAAGTTGA
ATGTGGAACAAATGGAAAGTTTGGCAGTCAGAAATTGTTTGATTGTTCTG
AAAAAAGAGGTGTTCTGATAGTCATAAATGACAATATTAGCACTTGTGTC
AATGCGCGTCATTGGGAAATTGCAGTTTGTCGCCATGATACGGTTGAAAA
GAACTCAATTTGCTGCACAATGTCTTTGAATGAGTTAAGTAAACATGCGT
ATTTTAAGATAGGTGTTAAAATTTGTAAGACATTAAAAAGTGAATCCAAA
TGCGGCACTTGCAAATGGGTTGGATTAGTAAATGGTGTCGAATGTATTGG
TGCAGAAATGGATTCACCACAGAAAAATGGTACTGCGGGCTCATTTAATG
GATTTAATTTATTTAATTGCCAAGGCAAACACGGGGTGATTTACAAGGCT
GATGGTGTCTCAGTTGTTAAAAATTAATTCTTGAAAATATGCTGACATAA
ATAACTTTGATATAATCTTTATTTTTTAAGTGATTATTTATCGTAAATTT
CATGAATTTTTATTTTGTGCCTTGTTTATATTTGTGAATTTGAATATAAT
TGCTGCTTAGGAACAAA
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protein sequence of SMED30015908-orf-1

>SMED30015908-orf-1 ID=SMED30015908-orf-1|Name=SMED30015908-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=211bp
MTMYKTIHYDKCELDVIDPLTISTDFEGEAENKQISVKMFNKLNVVWLKW
RGRVNGLLCIGCELKNKVECGTNGKFGSQKLFDCSEKRGVLIVINDNIST
CVNARHWEIAVCRHDTVEKNSICCTMSLNELSKHAYFKIGVKICKTLKSE
SKCGTCKWVGLVNGVECIGAEMDSPQKNGTAGSFNGFNLFNCQGKHGVIY
KADGVSVVKN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0002056medial muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR036859CAP-Gly_dom_sf
IPR000938CAP-Gly_domain
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000938CAP Gly-rich domainSMARTSM01052CAP_GLY_2coord: 139..206
e-value: 0.0019
score: 23.6
IPR000938CAP Gly-rich domainPFAMPF01302CAP_GLYcoord: 148..203
e-value: 6.8E-8
score: 32.4
IPR036859CAP Gly-rich domain superfamilyGENE3DG3DSA:2.30.30.190coord: 134..210
e-value: 3.4E-8
score: 35.1
IPR036859CAP Gly-rich domain superfamilySUPERFAMILYSSF74924Cap-Gly domaincoord: 133..208