SMED30015858

Overview
NameSMED30015858
Smed IDSMED30015858
Length (bp)1898
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30015858 (SMED30015858) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30015858 (SMED30015858) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30015858 (SMED30015858) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30015858 (SMED30015858) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30015858

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
testisSMED30015858SMESG000049146.1 Contig6142_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
early male germ cellSMED30015858SMESG000049146.1 Contig6142_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism asexual adult cDNA to DNA expression microarray evidence
testis primordiumSMED30015858SMESG000049146.1 Contig6142_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism juvenile cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Alignments
SMED30015858 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001241:78352..83506 -7329
Homology
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BLAST of SMED30015858 vs. Planmine SMEST
Match: SMESG000049146.1 (SMESG000049146.1)

HSP 1 Score: 467.618 bits (1202), Expect = 4.790e-163
Identity = 271/271 (100.00%), Postives = 271/271 (100.00%), Query Frame = 2 
Query:   83 MCTLEEDKKGFSLETCKSCGRNFNVDILDRHYPICQKVKAKPRKIIFDGSVGMTGKCLLASKSEEARSSHNSSLKEMKQIIGSIESEILPITEQLTNASSREAFQKRIQERDIFLSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTLRTGRSEFQYMGAQKEFMCKLSQSKIPKSTQWKIARVHLEPIPESRKSLVESNESAFNTIFGNQSQNRNPLSKNYPISTTQSILQKFANFCFQCGFKFSVEAAKFCSRCGTLRLAIE 895
            MCTLEEDKKGFSLETCKSCGRNFNVDILDRHYPICQKVKAKPRKIIFDGSVGMTGKCLLASKSEEARSSHNSSLKEMKQIIGSIESEILPITEQLTNASSREAFQKRIQERDIFLSKDLYNKKKMKQNKHKNDDWENSSSSISSSANADNSHDTLRTGRSEFQYMGAQKEFMCKLSQSKIPKSTQWKIARVHLEPIPESRKSLVESNESAFNTIFGNQSQNRNPLSKNYPISTTQSILQKFANFCFQCGFKFSVEAAKFCSRCGTLRLAIE
Sbjct:    1 MCTLEEDKKGFSLETCKSCGRNFNVDILDRHYPICQKVKAKPRKIIFDGSVGMTGKCLLASKSEEARSSHNSSLKEMKQIIGSIESEILPITEQLTNASSREAFQKRIQERDIFLSKDLYNKKKMKQNKHKNDDWENSSSSISSSANADNSHDTLRTGRSEFQYMGAQKEFMCKLSQSKIPKSTQWKIARVHLEPIPESRKSLVESNESAFNTIFGNQSQNRNPLSKNYPISTTQSILQKFANFCFQCGFKFSVEAAKFCSRCGTLRLAIE 271
[Close]
BLAST of SMED30015858 vs. Planmine SMEST
Match: SMESG000049146.1 (SMESG000049146.1)

HSP 1 Score: 462.611 bits (1189), Expect = 6.399e-161
Identity = 271/273 (99.27%), Postives = 271/273 (99.27%), Query Frame = 2 
Query:   83 MCTLEEDKKGFSLETCKSCGRNFNVDILDRHYPICQKVKAKPRKIIFDGSVGMTGKCLLASKSEEARSSHNSSLKEMKQIIGSIESEILPITEQLTN--ASSREAFQKRIQERDIFLSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTLRTGRSEFQYMGAQKEFMCKLSQSKIPKSTQWKIARVHLEPIPESRKSLVESNESAFNTIFGNQSQNRNPLSKNYPISTTQSILQKFANFCFQCGFKFSVEAAKFCSRCGTLRLAIE 895
            MCTLEEDKKGFSLETCKSCGRNFNVDILDRHYPICQKVKAKPRKIIFDGSVGMTGKCLLASKSEEARSSHNSSLKEMKQIIGSIESEILPITEQLTN  ASSREAFQKRIQERDIFLSKDLYNKKKMKQNKHKNDDWENSSSSISSSANADNSHDTLRTGRSEFQYMGAQKEFMCKLSQSKIPKSTQWKIARVHLEPIPESRKSLVESNESAFNTIFGNQSQNRNPLSKNYPISTTQSILQKFANFCFQCGFKFSVEAAKFCSRCGTLRLAIE
Sbjct:    1 MCTLEEDKKGFSLETCKSCGRNFNVDILDRHYPICQKVKAKPRKIIFDGSVGMTGKCLLASKSEEARSSHNSSLKEMKQIIGSIESEILPITEQLTNLDASSREAFQKRIQERDIFLSKDLYNKKKMKQNKHKNDDWENSSSSISSSANADNSHDTLRTGRSEFQYMGAQKEFMCKLSQSKIPKSTQWKIARVHLEPIPESRKSLVESNESAFNTIFGNQSQNRNPLSKNYPISTTQSILQKFANFCFQCGFKFSVEAAKFCSRCGTLRLAIE 273
The following BLAST results are available for this feature:
BLAST of SMED30015858 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015858 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015858 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015858 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015858 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015858 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015858 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015858 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015858 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015858 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015858 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015858 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015858 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015858 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000049146.14.790e-163100.00SMESG000049146.1[more]
SMESG000049146.16.399e-16199.27SMESG000049146.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015858 ID=SMED30015858|Name=SMED30015858|organism=Schmidtea mediterranea sexual|type=transcript|length=1898bp
TCTTAAACATTTGGTGTAAATAATCTAATTTAAATGGAAAGGAATATTGA
AATATTCGACCTTATCAAATAATCCTAACCAAATGTGCACCCTTGAAGAA
GATAAAAAGGGTTTCAGTTTAGAAACTTGTAAGTCTTGCGGTCGGAATTT
CAATGTGGATATTTTGGACAGGCATTATCCCATCTGTCAGAAAGTAAAAG
CTAAACCAAGGAAAATAATTTTTGATGGGTCTGTTGGTATGACTGGAAAA
TGCTTACTGGCATCAAAATCTGAGGAGGCGAGAAGTTCGCATAATTCGTC
ATTGAAAGAAATGAAACAAATTATCGGCAGTATCGAATCCGAAATTCTTC
CTATAACCGAACAATTAACAAACGCGAGCTCCAGAGAAGCCTTTCAGAAG
CGGATCCAAGAACGGGACATCTTTCTATCCAAGGATTTGTACAATAAGAA
AAAAATGAAACAGAATAAGCATAAAAATGATGACTGGGAAAACTCCTCTT
CCAGCATCAGCAGCAGCGCAAATGCCGACAATTCCCATGACACTTTAAGA
ACTGGCAGGTCGGAATTCCAATACATGGGAGCGCAGAAGGAATTCATGTG
CAAACTGTCTCAATCAAAAATTCCAAAGTCCACTCAATGGAAAATCGCAA
GGGTCCACCTGGAACCGATCCCCGAGTCCCGGAAGTCCTTAGTAGAGAGC
AATGAGAGCGCTTTCAACACTATATTTGGCAATCAAAGCCAGAATCGAAA
CCCGCTGAGTAAAAACTATCCTATTAGCACAACACAAAGTATTCTTCAGA
AATTTGCCAATTTTTGTTTTCAATGTGGATTCAAGTTTTCTGTTGAGGCG
GCGAAATTTTGCTCGCGGTGCGGAACGCTGCGTTTAGCAATCGAGTAAAT
GGAAGGAAACGATTGGGTGCTGGCAATTTGGTCAGAACCCTTCGCACTTT
CTGATCACACAATTTGTTGAAATCATTCAAATTTTACATGTAGACAAATA
TAAATTGAGAATATAAACTGCAAGTTAAGGTCTGTCCGGCATTTAAGCAA
GCAATGGGCATCACTAGATATAACTGTCACGGACGGGACGGATAGAGGGA
TTATTTTTAACACTCATGGGAACTAGGAGACTTGGGGCTCATTAATATTA
CGCAAGATTGGAACTAATGATAAATATGTGGAAAAATTGCAAATAAATAT
TTGAACTGAGAGGGAATTAACAATAACATTAAATCGGTCCAGAATAAATA
GACATGTGAATGTTACTGGATACGAGAGGGTGCTCATGAGCATTATTAAT
ATTTTTCGTGGATCACCAATAATATGACAAAGAATTATACGATAAGTATT
TAAAATAAAAGTATAAGTATAAAACGGTGATTCCTTGGTAGCAGCATAAA
TTAATATATCAGATAAGTGAATAACAAAATAAGCCCTTGCTGCGAAGTAA
TAATTATATTGTTGAAAAAAGTTTCGATTATTAAATAATCCTCTTCAGTT
TCTTGCAGAATTGTGGAGAAAGTCATAGGGAACAAAAGAATCAAAGAAAC
ATCTTGCAAACGTGTTTAGTTATCCAGTCAAGAGAATTGGTCTACTAAGT
ACAAAGACATGGATTTACCATAGTGGAGTCCCATTATTCCTTACGATTTA
ACAGGAGAATTAAACATCTTTGGCGAGCAGACATCACTAGGAGCGCACAA
ACACAATTCTGCAAGAAACTGAAGAGGATTATTATCGAAACTTGTTTCAA
CAATATAATTATTACTTTGCAGCAAGGGCTTATTTTATTATTTACTTATC
TGATATTTTAATTTATGCTGCTACCAAGGAATCACCGTTTTATACTTATT
ATTTATTTAATCCATTGTTTTCAGAATGCCGTCCGGAAATTAGGAGAC
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protein sequence of SMED30015858-orf-1

>SMED30015858-orf-1 ID=SMED30015858-orf-1|Name=SMED30015858-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=272bp
MCTLEEDKKGFSLETCKSCGRNFNVDILDRHYPICQKVKAKPRKIIFDGS
VGMTGKCLLASKSEEARSSHNSSLKEMKQIIGSIESEILPITEQLTNASS
REAFQKRIQERDIFLSKDLYNKKKMKQNKHKNDDWENSSSSISSSANADN
SHDTLRTGRSEFQYMGAQKEFMCKLSQSKIPKSTQWKIARVHLEPIPESR
KSLVESNESAFNTIFGNQSQNRNPLSKNYPISTTQSILQKFANFCFQCGF
KFSVEAAKFCSRCGTLRLAIE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000208testis
PLANA:0002030early male germ cell
PLANA:0003304testis primordium