HIT-type domain-containing protein

Overview
NameHIT-type domain-containing protein
Smed IDSMED30015846
Length (bp)1152
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of HIT-type domain-containing protein (SMED30015846) t-SNE clustered cells

Violin plots show distribution of expression levels for HIT-type domain-containing protein (SMED30015846) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of HIT-type domain-containing protein (SMED30015846) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for HIT-type domain-containing protein (SMED30015846) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30015846

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30015846SMESG000039467.1 SMESG000005538.1 SmedASXL_004978SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30015846SMESG000039467.1 SMESG000005538.1 Contig38075newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30015846SMESG000039467.1 SMESG000005538.1 Contig38075uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of HIT-type domain-containing protein vs. Ensembl Human
Match: ZNHIT2 (zinc finger HIT-type containing 2 [Source:HGNC Symbol;Acc:HGNC:1177])

HSP 1 Score: 88.1965 bits (217), Expect = 1.349e-18
Identity = 61/269 (22.68%), Postives = 107/269 (39.78%), Query Frame = 3 
Query:  129 KSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHV-SNEMKSKMEDILSKHSNPNIVPSNLTPESLEDRL-CDLALDETDEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWW-----VFEMVEEIDSKN-----------------------------LLRI---------PRIFDKINDIKMLSKHTNS-IIRYNIMSVLMSYVYICHRYNGDHHSFRDEIVDEIHEICPVLFSNMVFSNLEET--TGAFVLRASD 791
             ++Y CPRC+  YCS++CY +  H  C+ENFYRD  + EL+G     + + S +  +  +    +        E  E  L    A      +W  L   EK  F + +   E  + +  W+PWW       +++EE+D+                                 R+         P +  +I  I  LS+   S ++R+ + +VL +Y +    Y+G   +   +    +  +   L +  VF++ EE     A VL A +
Sbjct:   17 PARYTCPRCNAPYCSLRCYRT--HGTCAENFYRDQVLGELRGCSAPPSRLASALRRLRQQRETED--------EPGEAGLSSGPAPGGLSGLWERLAPGEKAAFERLLSRGEAGRLLPPWRPWWWNRGAGPQLLEELDNAPGSDAAELELAPARTPPDSVKDASAAEPAAAERVLGDVPGACTPVVPTRIPAIVSLSRGPVSPLVRFQLPNVLFAYAHTLALYHGGDDALLSDFCATLLGVSGALGAQQVFASAEEALQAAAHVLEAGE 275
[Close]
BLAST of HIT-type domain-containing protein vs. Ensembl Human
Match: ZNHIT2 (zinc finger HIT-type containing 2 [Source:HGNC Symbol;Acc:HGNC:1177])

HSP 1 Score: 50.8322 bits (120), Expect = 5.975e-7
Identity = 18/33 (54.55%), Postives = 25/33 (75.76%), Query Frame = 3 
Query:  132 SKYKCPRCDINYCSIKCYTSEKHNQCSENFYRD 230
            ++Y CPRC+  YCS++CY +  H  C+ENFYRD
Sbjct:   18 ARYTCPRCNAPYCSLRCYRT--HGTCAENFYRD 48
[Close]
BLAST of HIT-type domain-containing protein vs. Ensembl Celegans
Match: zhit-2 (Zinc finger, HIT-type [Source:UniProtKB/TrEMBL;Acc:Q7K7S3])

HSP 1 Score: 66.2402 bits (160), Expect = 1.393e-12
Identity = 51/177 (28.81%), Postives = 77/177 (43.50%), Query Frame = 3 
Query:   90 TDNIILCELCS-KLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHV-----SNEMKSKM------------------------EDI----------LSKHSNPNIVPSNLTPESLEDRLCDLAL-DETDEIWNILTSDEKEEFFKF---IESDEIRKFVKCWKP 488
            T + + C +C  + +  YKCPRCD+ YC+IKCY + KH++CSE FY++    EL G          E K KM                        ED+          + K     I    L    +E R+  L L D+ DE+ N LT +E+E F +    ++ +E+     C+ P
Sbjct:    3 TSSFLACGICGVEKREPYKCPRCDLLYCTIKCYRNPKHSECSEKFYQEQVKQELSGKKADISQKGEEYKEKMQKFLDGDWSGIEEGEPLDSDDEEQEDVADWQKEDTEAMKKTIQGTIDEYELEDGEIERRMTTLGLSDDIDELLNTLTPEEREAFKQLAAEMQEEELGLTNSCFAP 179
[Close]
BLAST of HIT-type domain-containing protein vs. Ensembl Fly
Match: CG31223 (gene:FBgn0051223 transcript:FBtr0475019)

HSP 1 Score: 107.842 bits (268), Expect = 9.608e-26
Identity = 74/277 (26.71%), Postives = 122/277 (44.04%), Query Frame = 3 
Query:  108 CELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHVSNEMKSKME---DILSKHS------NP-----------------------------NIVPSNLTPESLED----RLCDLALDETDEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWW--------VFEMVEEIDSKNLLRIPRIFDKINDIKMLSKHTNS-IIRYNIMSVLMSYVYICHRYNGDHHSFRDEIVDEIHEICPVLFSNMVFSNLEETTGAFVLRA 785
            C  C ++  KY CP+C+  YCS+ CY S++H +CSE FY+ C   EL    V+ E +  M    DIL +        NP                             N+   ++  E LE+    RL  + +++ DE+W+ LT++E++EF K I + +I K +  +KPWW        V     E  +K   R+P IF  I     + K T S  + YN+ ++L +Y  +   + G+  +   E V  +  +   L     F + E+   +  + A
Sbjct:    8 CHFCREVSFKYTCPKCNALYCSVGCYKSKEHLKCSEAFYKSCIQDELTQNTVTQESQEDMRKIYDILKRMRETDSGFNPQEFDTDGLENSLDSDGGEEGESEEDEAEENVTEGDILDEDLEEDIATRLKGVDINDADEVWSRLTTEEQDEFQKLITNGDIMKLMPDYKPWWIKPKNSKIVIIPTPEEAAKKESRVPEIFGSIAKFSDICKKTPSPCLHYNLWNILSAYACVARFFGGEQRTNASEAVAHLVNLSATLKYGTNFEDAEDAIVSVEMEA 284
[Close]
BLAST of HIT-type domain-containing protein vs. Ensembl Zebrafish
Match: BX248510.4 (pep chromosome:GRCz11:CHR_ALT_CTG10_2_4:26977650:26983865:-1 gene:ENSDARG00000110088.1 transcript:ENSDART00000192365.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX248510.4)

HSP 1 Score: 102.449 bits (254), Expect = 2.127e-23
Identity = 90/400 (22.50%), Postives = 156/400 (39.00%), Query Frame = 3 
Query:   96 NIILCELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQG------------------VHVSNEMKSKMEDILSKHSNPNIVPSNLTPESLEDRLCDLA----------------------------------------LD----ETDEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWW------VFEMVEEIDSKNLLR--------------------IPRIFDKINDIKMLSKHTNSIIRYNIMSVLMSYVYICHRYNGDHHSFRDEIVDEIHEICPVLFSNMVFSNLEETTGAFVLRASDAKIQFNN-----AMXXXXXXXXXXXXRAEETGRTFILSALSDFRLILKSGR--------AKMRQSY----KKVEFYISWL 980
            +I  C LC    S Y CPRC+I YC + CY S+ H++CSE FY++  + EL+                   +  S + +  M++ L      N+   +   ++L D LC LA                                        LD      +++W++L++ EKE+F   ++   I   V  W+PWW         ++EE+  +N +                     +P I  KI  +  L+ + + ++++N+++ L  Y +    +NGD      E    +  I   L +  VF+++ E   A + R+  A   F+       +  +         R+ E    + LSALS  R  L   +         +MRQ +    KK EF+ SW+
Sbjct:   74 SIKPCGLCLIKPSSYTCPRCNIPYCGLACYKSQSHSKCSEEFYKESVLQELKSRGATDEEGKSKMQEILLRLRQSADTEGGMQNFLGHLDGTNVTEGDA--QAL-DLLCKLAELQSGGDENSHEAQEILAKLEEADDDDDDDDEDLAEKLAGLDVDSLSEEQLWSLLSTQEKEKFENLLKVGGIAGMVVLWRPWWEQHEKETKTLIEELRFENQVEVRTAKKGKPQKVASDAKQSAVPPISAKIPPLHRLTSNPSPLVQFNLINALYGYTFSLCLFNGDISDTLLEFSQALVSISEFLGAGRVFNSVPEALDAGI-RSVSAGGYFDREDSSAPIRAVEAVAHVLTGRSREDAEGYTLSALSQVRSALSKAKMAAATDDDERMRQGFFQAGKKCEFFQSWV 469
[Close]
BLAST of HIT-type domain-containing protein vs. Ensembl Zebrafish
Match: znhit2 (zinc finger, HIT-type containing 2 [Source:ZFIN;Acc:ZDB-GENE-061103-46])

HSP 1 Score: 102.449 bits (254), Expect = 2.508e-23
Identity = 90/400 (22.50%), Postives = 156/400 (39.00%), Query Frame = 3 
Query:   96 NIILCELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQG------------------VHVSNEMKSKMEDILSKHSNPNIVPSNLTPESLEDRLCDLA----------------------------------------LD----ETDEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWW------VFEMVEEIDSKNLLR--------------------IPRIFDKINDIKMLSKHTNSIIRYNIMSVLMSYVYICHRYNGDHHSFRDEIVDEIHEICPVLFSNMVFSNLEETTGAFVLRASDAKIQFNN-----AMXXXXXXXXXXXXRAEETGRTFILSALSDFRLILKSGR--------AKMRQSY----KKVEFYISWL 980
            +I  C LC    S Y CPRC+I YC + CY S+ H++CSE FY++  + EL+                   +  S + +  M++ L      N+   +   ++L D LC LA                                        LD      +++W++L++ EKE+F   ++   I   V  W+PWW         ++EE+  +N +                     +P I  KI  +  L+ + + ++++N+++ L  Y +    +NGD      E    +  I   L +  VF+++ E   A + R+  A   F+       +  +         R+ E    + LSALS  R  L   +         +MRQ +    KK EF+ SW+
Sbjct:   74 SIKPCGLCLIKPSSYTCPRCNIPYCGLACYKSQSHSKCSEEFYKESVLQELKSRGATDEEGKSKMQEILLRLRQSADTEGGMQNFLGHLDGTNVTEGDA--QAL-DLLCKLAELQSGGDENSHEAQEILAKLEEADDDDDDDDEDLAEKLAGLDVDSLSEEQLWSLLSTQEKEKFENLLKVGGIAGMVVLWRPWWEQHEKETKTLIEELRFENQVEVRTAKKGKPQKVASDAKQSAVPPISAKIPPLHRLTSNPSPLVQFNLINALYGYTFSLCLFNGDISDTLLEFSQALVSISEFLGAGRVFNSVPEALDAGI-RSVSAGGYFDREDSSAPIRAVEAVAHVLTGRSREDAEGYTLSALSQVRSALSKAKMAAATDDDERMRQGFFQAGKKCEFFQSWV 469
[Close]
BLAST of HIT-type domain-containing protein vs. Ensembl Zebrafish
Match: BX088530.2 (pep chromosome:GRCz11:CHR_ALT_CTG10_1_25:26947297:26952654:-1 gene:ENSDARG00000110030.1 transcript:ENSDART00000185993.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX088530.2)

HSP 1 Score: 96.6709 bits (239), Expect = 1.873e-21
Identity = 96/397 (24.18%), Postives = 160/397 (40.30%), Query Frame = 3 
Query:   96 NIILCELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHVSN-EMKSKMEDIL--------SKHSNPNIV----PSNLTPESLE--DRLCDLA-----LDE-TDEIWNILT--------------------------SDEKEEFFKFIESDEIRKF------------VKCWKPWW------VFEMVEEIDSKNLLR--------------------IPRIFDKINDIKMLSKHTNSIIRYNIMSVLMSYVYICHRYNGDHHSFRDEIVDEIHEICPVLFSNMVFSNLEETTGAFVLRASDAKIQFNN-----AMXXXXXXXXXXXXRAEETGRTFILSALSDFRLILKSGR--------AKMRQSY----KKVEFYISWL 980
            +I  C LC   +S Y CPRC+I YC + CY S+ H++CSE FY++  + EL+    ++ E KSKM++IL        ++    N +     +N+T    +  D LC LA      DE + E   IL                           S  +E+ +  + + E  KF            V  W+PWW         ++EE+  +N +                     +P I  KI  +  L+ + + ++++N+++ L  Y +    +NGD      E    +  I   L +  VF+++ E   A + R+  A   F+       +  +         R+ E    + LSALS  R  L   +         +MRQ +    KK EF+ SW+
Sbjct:   74 SIKPCGLCLLKQSSYTCPRCNIPYCGLACYKSQSHSKCSEEFYKESVLQELKSRGATDEEGKSKMQEILLRLRQSADTEGGMQNFLRHLDGTNVTEGDAQALDLLCKLAELQSGGDENSHEAQEILAKLEEADDDDDDDDEDLAEKLAGLDVDSLSEEQLWSLLSTQEKEKFENLLKVGGIAGMVVLWRPWWEQHEKETKTLIEELRFENQVEVRTAKKGKPQKVASDAKQSAVPPISAKIPPLHRLTSNPSPLVQFNLINALYGYTFSLCLFNGDISDTLLEFSQALVSISEFLGAGRVFNSVPEALDAGI-RSVSAGGYFDREDSSAPIRAVEAVAHVLTGRSREDAEGYTLSALSQLRSALSKAKMAAAKDDDERMRQGFFQAGKKCEFFQSWV 469
[Close]
BLAST of HIT-type domain-containing protein vs. Ensembl Xenopus
Match: dnajc7 (DnaJ heat shock protein family (Hsp40) member C7 [Source:Xenbase;Acc:XB-GENE-946345])

HSP 1 Score: 90.8929 bits (224), Expect = 2.860e-19
Identity = 42/137 (30.66%), Postives = 68/137 (49.64%), Query Frame = 3 
Query:   93 DNIILCELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHVSNEMKSKMEDILSKHSNPNIVPSNLTPESLEDRLCDLALDETDEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWWVFE 503
            + + +C LC     +Y CPRC+  YCS+ CY   +H  CSE+FY++  +  L+        K ++E++L K     I   +       D L     +E   +WN LT  E+++F + ++S +I   V  W PWW  E
Sbjct:   30 EGLAVCALCLSNPGRYTCPRCNSPYCSLACYRGPRHQTCSEDFYKESVLQMLREEQAGAAGKRQVEEMLVKLREKAIAGDDAVGLDGIDSLG----EEAANLWNRLTPSEQKDFNRLLQSGDIGALVPKWNPWWEVE 162
[Close]
BLAST of HIT-type domain-containing protein vs. Ensembl Mouse
Match: Znhit2 (zinc finger, HIT domain containing 2 [Source:MGI Symbol;Acc:MGI:1352481])

HSP 1 Score: 93.9745 bits (232), Expect = 1.017e-20
Identity = 75/350 (21.43%), Postives = 131/350 (37.43%), Query Frame = 3 
Query:  105 LCELCSK---LKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHVSNEMKSKMEDILSKHSNPNIVP--SNLTPESLEDRLCDLALDETDEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWW-----VFEMVEEID------------------------------------SKNLLRIPRIFDKINDIKMLSKHTNS-IIRYNIMSVLMSYVYICHRY-NGDHHSFRDEIVDEIHEICPVLFSNMVFSNLEET--TGAFVLRASDAKIQFNNAMXXXXXXXXXXXXRAEETGRTFILSALSDF---------RLILKSGRAKMRQSYKKVEFYISW 977
            LC  C     L ++Y CPRC+  YCS++CY +  H  C+E+FYRD  + EL+G   S    +     L +       P  + L P +    L  L        W  LT  EK  F + +   E  + +  W+PWW        ++EE+D                                    +   L +P    +I  +  LS+   S ++R+ + +VL +Y +    Y  GD  +   +    + ++   L +  VF + EE     A VL A +           +               + + L+AL            + ++   R ++ ++ KK +F ++W
Sbjct:    6 LCGFCPAGEALPARYTCPRCNAPYCSLRCYRA--HGACAEDFYRDQVLRELRGRSASPSRLAGALRRLREQREAEDEPEEAGLGPGARPGGLSGL--------WERLTPAEKAAFERLLSRGEAGRLLPPWRPWWWGRGTGPRLLEELDHAANRDLAEPEPAPARTALQSGDDAAAAEPFAEDSCAARPLALP---ARIPALASLSRSPASPLVRFQLPNVLFAYAHTLALYHGGDDDALLSDFCATLLDVSGALGAQQVFGSTEEALQAAAHVLEAGEHPPGPLGTRGAMQEVARILLGEGPVNQKGYTLTALGHLAQTLGRARKQAVIGGERDRLYRARKKCQFLLAW 342
[Close]
BLAST of HIT-type domain-containing protein vs. UniProt/SwissProt
Match: sp|Q2TBW5|ZNHI2_BOVIN (Zinc finger HIT domain-containing protein 2 OS=Bos taurus OX=9913 GN=ZNHIT2 PE=2 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 1.611e-21
Identity = 74/343 (21.57%), Postives = 130/343 (37.90%), Query Frame = 3 
Query:  108 CELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHVSNEMKSKMEDILSKHSNPNIVP--SNLTPESLEDRLCDLALDETDEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWW-----VFEMVEEI------DSKNL----LRI------------------------PRIFDKINDIKMLSK-HTNSIIRYNIMSVLMSYVYICHRYNGDHHSFRDEIVDEIHEICPVLFSNMVFSNLEET--TGAFVLRASDAKIQFNNAMXXXXXXXXXXXXRAEETGRTFILSALSDFRLILKSGRA---------KMRQSYKKVEFYISW 977
            C       ++Y CPRC++ YCS++CY +  H  C+E FYRD  + EL+G   S    +     L +       P  + L P      L  L        W  L   EK  F + +   E  + +  W+PWW        ++EE+      D++ L     R+                        P +  +I  +  LS+  T+ ++R+ + +VL +Y +    Y+G   +   +    +  +   L +  VF++ EE     A VL A +           +               +++ L+AL D    L   R          ++ ++ KK +F +SW
Sbjct:   10 CPTGEAQPARYTCPRCNVPYCSLRCYRA--HGSCAEEFYRDQVLGELRGRSASPSRLATALRRLRQQRETEDEPGDAGLRPGPAPGGLSGL--------WERLAPAEKVAFERLLSRGEAGRLLPPWRPWWWGRGAGPRLLEELGDAPSGDAEELEPSPARMPPEPVRDEPAAVEQVLGDLPGACPPAVPTRIPALASLSRGRTSPLVRFQLPNVLFAYAHTLALYHGGDEALLSDFCATLLGVSGALGAQQVFASAEEALQAAAHVLEAGEHPPGPLGTRGAMREAARILLGEGPANQKSYTLAALGDLAQTLGRARKQAVAPEERDRLYRARKKCQFLLSW 342
[Close]
BLAST of HIT-type domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9QY66|ZNHI2_MOUSE (Zinc finger HIT domain-containing protein 2 OS=Mus musculus OX=10090 GN=Znhit2 PE=2 SV=2)

HSP 1 Score: 93.9745 bits (232), Expect = 7.111e-20
Identity = 75/350 (21.43%), Postives = 131/350 (37.43%), Query Frame = 3 
Query:  105 LCELCSK---LKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHVSNEMKSKMEDILSKHSNPNIVP--SNLTPESLEDRLCDLALDETDEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWW-----VFEMVEEID------------------------------------SKNLLRIPRIFDKINDIKMLSKHTNS-IIRYNIMSVLMSYVYICHRY-NGDHHSFRDEIVDEIHEICPVLFSNMVFSNLEET--TGAFVLRASDAKIQFNNAMXXXXXXXXXXXXRAEETGRTFILSALSDF---------RLILKSGRAKMRQSYKKVEFYISW 977
            LC  C     L ++Y CPRC+  YCS++CY +  H  C+E+FYRD  + EL+G   S    +     L +       P  + L P +    L  L        W  LT  EK  F + +   E  + +  W+PWW        ++EE+D                                    +   L +P    +I  +  LS+   S ++R+ + +VL +Y +    Y  GD  +   +    + ++   L +  VF + EE     A VL A +           +               + + L+AL            + ++   R ++ ++ KK +F ++W
Sbjct:    6 LCGFCPAGEALPARYTCPRCNAPYCSLRCYRA--HGACAEDFYRDQVLRELRGRSASPSRLAGALRRLREQREAEDEPEEAGLGPGARPGGLSGL--------WERLTPAEKAAFERLLSRGEAGRLLPPWRPWWWGRGTGPRLLEELDHAANRDLAEPEPAPARTALQSGDDAAAAEPFAEDSCAARPLALP---ARIPALASLSRSPASPLVRFQLPNVLFAYAHTLALYHGGDDDALLSDFCATLLDVSGALGAQQVFGSTEEALQAAAHVLEAGEHPPGPLGTRGAMQEVARILLGEGPVNQKGYTLTALGHLAQTLGRARKQAVIGGERDRLYRARKKCQFLLAW 342
[Close]
BLAST of HIT-type domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9UHR6|ZNHI2_HUMAN (Zinc finger HIT domain-containing protein 2 OS=Homo sapiens OX=9606 GN=ZNHIT2 PE=1 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 6.478e-18
Identity = 61/269 (22.68%), Postives = 107/269 (39.78%), Query Frame = 3 
Query:  129 KSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHV-SNEMKSKMEDILSKHSNPNIVPSNLTPESLEDRL-CDLALDETDEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWW-----VFEMVEEIDSKN-----------------------------LLRI---------PRIFDKINDIKMLSKHTNS-IIRYNIMSVLMSYVYICHRYNGDHHSFRDEIVDEIHEICPVLFSNMVFSNLEET--TGAFVLRASD 791
             ++Y CPRC+  YCS++CY +  H  C+ENFYRD  + EL+G     + + S +  +  +    +        E  E  L    A      +W  L   EK  F + +   E  + +  W+PWW       +++EE+D+                                 R+         P +  +I  I  LS+   S ++R+ + +VL +Y +    Y+G   +   +    +  +   L +  VF++ EE     A VL A +
Sbjct:   17 PARYTCPRCNAPYCSLRCYRT--HGTCAENFYRDQVLGELRGCSAPPSRLASALRRLRQQRETED--------EPGEAGLSSGPAPGGLSGLWERLAPGEKAAFERLLSRGEAGRLLPPWRPWWWNRGAGPQLLEELDNAPGSDAAELELAPARTPPDSVKDASAAEPAAAERVLGDVPGACTPVVPTRIPAIVSLSRGPVSPLVRFQLPNVLFAYAHTLALYHGGDDALLSDFCATLLGVSGALGAQQVFASAEEALQAAAHVLEAGE 275
[Close]
BLAST of HIT-type domain-containing protein vs. TrEMBL
Match: K1QXW4 (Zinc finger HIT domain-containing protein 2 OS=Crassostrea gigas OX=29159 GN=CGI_10028122 PE=4 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 2.049e-38
Identity = 101/321 (31.46%), Postives = 169/321 (52.65%), Query Frame = 3 
Query:  108 CELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHVSNEMKSKMEDILSKHSNPNIVPSNLTP---ESLEDRLCDLALD-ETDEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWWVFEMVE--EIDSKNL-LRIPRIFDKINDI-KMLSKHTNSI-IRYNIMSVLMSYVYICHRYNGDHHSFRDEIVDEIHEICPVLFSNMVFSNLEETTGAFVLRASDAKIQ-FNNAMXXXXXXXXXXXXRAEETGRTFILSALSDFRLILKSGRAKMRQ---------------------SYKKVEFYISW 977
            C++C+K ++KY CPRC++ YC + CY  ++H+ CSE FY++CCM  L+   VS+E K KM ++L +     +  S+      E L +RL  L LD ++++IWN LTS E+ +F + ++   +   V+ W PWW  + V+  ++DS+ L  + P +  KI DI K+L+K   S  IR N+++VL +YV++C  YNG H     E   ++ +   VL  N V S+  E     ++ +   + Q F   ++  +   L    R EE    F+++ALS+   +LK      ++                     + KK+EFY+ W
Sbjct:    7 CKICNKKQAKYTCPRCNLGYCGLSCYRHKEHSDCSEAFYKECCMEALEDQDVSSEEKQKMLEMLDRLEKEEVQNSDSNSDEGEELSERLSGLNLDTDSEKIWNRLTSKEQAQFNQMMKDGRLGNLVEVWTPWWDVKKVKIRDVDSEKLDDKTPDVLSKIPDISKLLTKSKPSADIRNNLVNVLFAYVFVCRLYNGTHWDCPRESALKLVKGSDVLSKNSVCSSTSEAIQLGLINSECKQSQEFLIELVSDVTKILRGPGRGEEL--KFVMAALSEILQLLKKCHTLYKKDIKVYPASSTDDREEKQLLFLAIKKLEFYLGW 325
[Close]
BLAST of HIT-type domain-containing protein vs. TrEMBL
Match: A0A1B6G750 (HIT-type domain-containing protein (Fragment) OS=Cuerna arida OX=1464854 GN=g.19432 PE=4 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 1.085e-36
Identity = 82/244 (33.61%), Postives = 135/244 (55.33%), Query Frame = 3 
Query:  105 LCELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHVSNEMKSKMEDILSK---HSNPNIVPSN--LTPESLEDRLCDLALDETDEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWWVF----EMVEEIDSKNLLRIPRIFDKINDIKMLSKHTNSI--------IRYNIMSVLMSYVYICHRYNGDHHSFRDEIVDEIHEICPVLFSNMVFSNLEETTGAFVLRA 785
            LC++C + +SKY CP+C+I YCS++CY SE H QCSE+FYR C + +++      E   +ME+IL +   +S  + V S+   T E L +RL  + LD+T+ +W ILT DE+++F KF+ + + R  +  W PWW +    +++E+++   L   P+       IK   +H + I        +RY++ +V+ +Y      YNGDH S   E    +  +   L ++ +F N EET  + V  A
Sbjct:    6 LCKICGERQSKYICPQCNIAYCSVQCYQSEVHAQCSESFYRKCVVEQMKCEESDPETHRRMEEILQRNLLYSEIDYVDSDDSDTCEDLVERLAGVDLDDTETVWGILTQDERKQFQKFVSTGDFRAVMPVWNPWWRYRKIKKLIEDLEESVLETNPQAKHYTPTIKQDIQHMSQICRVEPSPLVRYSVANVMAAYAVTTRVYNGDHQSVPLEAACMLCMLSGSLQNSQIFDN-EETAVSSVYMA 248
[Close]
BLAST of HIT-type domain-containing protein vs. TrEMBL
Match: A0A1B6J203 (HIT-type domain-containing protein OS=Homalodisca liturata OX=320908 GN=g.9622 PE=4 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 1.409e-36
Identity = 91/306 (29.74%), Postives = 155/306 (50.65%), Query Frame = 3 
Query:  108 CELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHVSNEMKSKMEDIL-------SKHSNPNIVPSNLTPE--SLEDRLCDLALDETDEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWWVF----EMVEEIDSKNLLRI--------PRIFDKINDIKMLSKHTNS-IIRYNIMSVLMSYVYICHRYNGDHHSFRDEIVDEIHEICPVLFSNMVFSNLEETTGAFVLRASDAKIQFNNAMXXXXXXXXXXXXRAEETGR--TFILSALSDFRLILKSGRAKMRQSYK 953
            C++C ++++KY CPRC+I YCS+ CY SE H QCSE+FYRDC + +++G  + +E + KM ++L       ++  +   + S+ +     L DRL  + LD  D +W  LT DE+++F +F+ S +I + +  W PWW +    E+V+E++  + +          P I   I  +  LSK T S ++R+N+++VL +Y      YNGD  S        +  I   L  N VF N E    +  + A +     +      + +D+   +      R    +L+ALS    IL   +  M+++ K
Sbjct:    7 CKMCLQIEAKYVCPRCNIPYCSLSCYQSESHVQCSESFYRDCVVEQIRGDQLDSESQQKMVEMLQRVQQIDTQFQDEEALDSDDSDSCAELSDRLAGVDLDNADSVWERLTEDERQQFHQFVTSGDIGELLPEWTPWWTYREKKELVKELNVSSSVEEESELASHCPSIKQDIQLLSKLSKMTPSPMVRWNVVNVLAAYAVTARVYNGDFQSSPQSAAVMLITISENLAINQVFDNPELAVTSVQMAAINYGCFLDGVEGTTLKEDVKMLVEGPSESRQNQHVLAALSHIHSILTDAKRIMKETTK 312
[Close]
BLAST of HIT-type domain-containing protein vs. TrEMBL
Match: D2A4C1 (Zinc finger HIT domain-containing protein 2-like Protein OS=Tribolium castaneum OX=7070 GN=TcasGA2_TC014859 PE=4 SV=2)

HSP 1 Score: 143.28 bits (360), Expect = 1.788e-36
Identity = 82/233 (35.19%), Postives = 129/233 (55.36%), Query Frame = 3 
Query:   93 DNIILCELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHVSNEMKSKMEDIL--SKHSNPNIVPSNLTPESLEDRLCDLALDETDEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWWVFEM--VEEIDSKNLLR--IPRIFDKINDIKMLSKHTNS-IIRYNIMSVLMSYVYICHRYNGDHHSFRDEIVDEIHEICPVLFSNMVFSNLEETTGA 770
            D    CE C    +KY CP+C   YC++ CY SEKH QCSE FY+DC + EL     + E K KM +IL  S      +   +     + DRL  + LD+ D++W  LT DE++EF  F++S+++ K +  W+PWW  +   VEEI+S  L +   P++  KI D + +SK   +  ++YN+++VL +Y +    +NG+HH F  E++  +  I   L +N  F + E    +
Sbjct:   10 DETNTCEFCCNALAKYTCPKCQSKYCTLVCYQSEKHLQCSEEFYKDCIVKEL-AQEDNEEAKKKMLEILKRSHLEEEELDSDDDDFADIADRLAGVDLDDADQVWEKLTKDEQQEFVAFLKSEDVTKLIPSWEPWWSHKSPNVEEIESCQLYKSECPQLI-KIRDFREISKKNPADCVKYNLINVLAAYAFATRYFNGEHHDFAKEVISCVVTISLTLKNNQNFDDYESAVKS 240
[Close]
BLAST of HIT-type domain-containing protein vs. TrEMBL
Match: A0A1J1HLD9 (CLUMA_CG002561, isoform A OS=Clunio marinus OX=568069 GN=CLUMA_CG002561 PE=4 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 2.690e-36
Identity = 94/306 (30.72%), Postives = 154/306 (50.33%), Query Frame = 3 
Query:  108 CELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHVSNEMKSKMEDILSK-HSNPN-------IVPSNLTPESLEDRLCDLALDETDEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWWVFEMVEEI------DSKNLLRI----PRIFDKINDIKMLS-KHTNSIIRYNIMSVLMSYVYICHRYNGDHHSFRDEIVDEIHEICPVLFSNMVFSNLEETTGAFVLRASDAK----IQFNNAMXXXXXXXXXXXXRAEETGRTFILSALSDFRLILKSGRAKMRQSYKK 956
            CE+C     KY CPRC+I YCS+ CY SE H QCSE FY++C + ELQ    + +   K+ ++L +    P                  LE+RL ++ L+  D +W+ LT +EK+EF   + + EI K V+  KPWW   + E++      D + +  I    P++F  + + + +S K   + I YNI +V+ +Y YI   YNGDH+S+  E  + +  IC  L SN  F ++     + ++   +A     +   +AM   + +       ++   +++ILS LSD   + KS +   RQ+  K
Sbjct:    3 CEICQSQSFKYSCPRCNIKYCSLDCYRSENHGQCSEAFYKECVLDELQHQKANKDDTRKILEMLQRIEDEPEQDFESLDSDDDESDVGDLEERLAEVNLNNADAVWDKLTDEEKQEFKSIVFNGEIDKIVQPVKPWWNNHLDEKLVRDIQEDERKITEILKECPKVFANVKEFQKISTKKPAACIIYNIANVIAAYTYIFRYYNGDHNSYDLEASNNLITICENLKSNANFDSISLAVDSVMMECHNANLFSDLSTKDAMHEDLKEIFNGPG-SKSHEKSYILSVLSDIINLFKSAKQSYRQNNPK 307
[Close]
BLAST of HIT-type domain-containing protein vs. Ensembl Cavefish
Match: znhit2 (zinc finger HIT-type containing 2 [Source:NCBI gene;Acc:103043260])

HSP 1 Score: 59.6918 bits (143), Expect = 2.650e-9
Identity = 28/79 (35.44%), Postives = 44/79 (55.70%), Query Frame = 3 
Query:  105 LCELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHVS-NEMKSKMEDILSKHSNPNIVPSNL 338
            LC LC      Y CPRC++ YC + CY    H+ CSE FY++    EL+   V+ +E +++M++IL +  +   V   L
Sbjct:   63 LCGLCLSKSCCYTCPRCNVPYCGLSCYRGPAHSACSEEFYKEFVSEELKCSDVTGDEGRAQMQEILLRLKSSAAVSGGL 141

HSP 2 Score: 48.9062 bits (115), Expect = 6.245e-6
Identity = 71/365 (19.45%), Postives = 139/365 (38.08%), Query Frame = 3 
Query:  174 IKCYTSEKHNQCSE-NFYRDCCMSELQGVHVSNEMKSKMEDILSKHSNPNIVPSNLTPESLEDRLCDLALDET---------------DEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWW------VFEMVEEIDSK---------------------------NLLRI------PRIFDKINDIKMLSKHTNS-----------------------IIRYNIMSVLMSYVYICHRYNGDHHSFRDEIVD---EIHEICPVLFSNMVFSNLEET-TGAFVLRASDA---KIQFNNAMXXXXXXXXXXXXRAEETGRTFILSALSDFRLILKSGRAKMRQ-----------SYKKVEFYISWL 980
            +KC   E     +E +F     ++ L  +  S + + ++++IL K  N    P +   E  E+   +   DET               +E+W++L S +KE+F + + +  I   V  W+PWW          VEE+ S+                           NL  +      P++   +    +LSK                           ++++++++ L  Y +    +NGD  S  ++++D    + EI   L +  VFS+L E   GA +  ++     +   +  +  L         ++++    + LSAL+   ++ K  RA + +           + KK EF+ SW+
Sbjct:  145 LKCEKEESREDVTEKDFVALELLTRLAKLQASGKEEKEVQEILQKLKNMEEGPHSEEEEEEEEEEEEEEKDETLACKLSGVNINSLTEEELWDLLPSQDKEQFERLVMAGGIAGLVPLWRPWWERHEQTTKSFVEELQSEKDEAQLSDQKGGQGFTGQNANKETQMNNLQPVQTDQCPPQVRQGLKKQNVLSKKKGGPNSAPLISTKILPLHKLSSSHSPLVQFSMVNTLYGYAFSLCLFNGD-ISESEQMLDFCRTVLEISESLDTGRVFSSLSEALEGATIAVSASGCSDRSDLDAPVRALHAVAHILTGQSKDDPTGYSLSALTQLWMVFKEARASVSKEEKQWKRRFFLAGKKCEFFQSWV 508
[Close]
BLAST of HIT-type domain-containing protein vs. Ensembl Yeast
Match: HIT1 (Protein involved in C/D snoRNP assembly; regulates abundance of Rsa1p; required for growth at high temperature; similar to human ZNHIT3 [Source:SGD;Acc:S000003816])

HSP 1 Score: 45.4394 bits (106), Expect = 2.268e-6
Identity = 17/39 (43.59%), Postives = 25/39 (64.10%), Query Frame = 3 
Query:   87 MTDNIILCELCSKLKSKYKCPRCDINYCSIKCYT-SEKH 200
            M  + + C +C  +  KYKCP+C + YCS+KCY  + KH
Sbjct:    1 MVSSAVKCGICRGVDGKYKCPKCGVRYCSLKCYKDAAKH 39
[Close]
BLAST of HIT-type domain-containing protein vs. Ensembl Nematostella
Match: EDO42122 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2D2])

HSP 1 Score: 60.8474 bits (146), Expect = 2.011e-10
Identity = 58/251 (23.11%), Postives = 107/251 (42.63%), Query Frame = 3 
Query:  348 SLEDRLCDLALD-ETDEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWWVFEMVEEID------------------SKNLLRIPRIFDKINDIKMLSK--HTNSIIRYNIMSVLMSYVYICHRYNGDHHSFRDEIVDEIHEICPVLFSNMVFSNLEETTGAFVLRASDAKIQFNN-------AMXXXXXXXXXXXXRAEETGRTFILSALSDFRLILKSGRAKMRQ-------------SYKKVEFYISW 977
             LE+RL D+ LD + D +W+ L   E++ F   ++S +    +   +PWW  E+ + ++                  SK   +IP + DK+  +  + K    N  ++++I+ VL SY Y    YN        +  +    +  V+  N+ F + +      +  + + K  FN+        + ++ I + F    +EE   +F+  ALSD   +L  G   +RQ             + KKV+F +SW
Sbjct:   38 GLEERLRDIDLDRDPDVLWDALGKAEQQGFLSALKSGKFSHSITLLEPWWKPEIKKHVEVLPPLEESNLDHHYTTSLSKTKSKIPSLIDKVPPLMAIIKTAQPNPAVKFSIVDVLYSYAYTMRLYNFTPQDDPRQASETFLALSSVISKNITFQSTDTVAHTSLNNSMEQKDLFNSREFSCNILLDVVEIINGFTSDTSEEV--SFVSLALSDCHRLLSRGLKALRQEQGDKDLEKKLWLAKKKVDFLLSW 286
[Close]
BLAST of HIT-type domain-containing protein vs. Ensembl Medaka
Match: znhit2 (zinc finger HIT-type containing 2 [Source:NCBI gene;Acc:101158226])

HSP 1 Score: 63.929 bits (154), Expect = 9.517e-11
Identity = 26/70 (37.14%), Postives = 42/70 (60.00%), Query Frame = 3 
Query:   93 DNIILCELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGV-HVSNEMKSKMEDIL 299
            + I  C  C    S+Y CPRC++ YC + CY S +H  CSE FY++  + EL+ +    +E + +M++IL
Sbjct:   69 NTITACTFCKCKPSRYTCPRCNLQYCGLACYQSREHTSCSEEFYKESVLQELKKMGKTESEGRRRMQEIL 138
[Close]
BLAST of HIT-type domain-containing protein vs. Planmine SMEST
Match: SMESG000039467.1 (SMESG000039467.1)

HSP 1 Score: 611.298 bits (1575), Expect = 0.000e+0
Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 3 
Query:   87 MTDNIILCELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHVSNEMKSKMEDILSKHSNPNIVPSNLTPESLEDRLCDLALDETDEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWWVFEMVEEIDSKNLLRIPRIFDKINDIKMLSKHTNSIIRYNIMSVLMSYVYICHRYNGDHHSFRDEIVDEIHEICPVLFSNMVFSNLEETTGAFVLRASDAKIQFNNAMXXXXXXXXXXXXRAEETGRTFILSALSDFRLILKSGRAKMRQSYKKVEFYISWLSTSRQFQGFXXXXXXXXXXXXXXSFRLK 1064
            MTDNIILCELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHVSNEMKSKMEDILSKHSNPNIVPSNLTPESLEDRLCDLALDETDEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWWVFEMVEEIDSKNLLRIPRIFDKINDIKMLSKHTNSIIRYNIMSVLMSYVYICHRYNGDHHSFRDEIVDEIHEICPVLFSNMVFSNLEETTGAFVLRASDAKIQFNNAMLLLIIDDLFYFLRAEETGRTFILSALSDFRLILKSGRAKMRQSYKKVEFYISWLSTSRQFQGFVEELKNELEVVKMVSFRLK
Sbjct:    1 MTDNIILCELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHVSNEMKSKMEDILSKHSNPNIVPSNLTPESLEDRLCDLALDETDEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWWVFEMVEEIDSKNLLRIPRIFDKINDIKMLSKHTNSIIRYNIMSVLMSYVYICHRYNGDHHSFRDEIVDEIHEICPVLFSNMVFSNLEETTGAFVLRASDAKIQFNNAMLLLIIDDLFYFLRAEETGRTFILSALSDFRLILKSGRAKMRQSYKKVEFYISWLSTSRQFQGFVEELKNELEVVKMVSFRLK 326
[Close]
BLAST of HIT-type domain-containing protein vs. Planmine SMEST
Match: SMESG000005538.1 (SMESG000005538.1)

HSP 1 Score: 605.134 bits (1559), Expect = 0.000e+0
Identity = 324/326 (99.39%), Postives = 324/326 (99.39%), Query Frame = 3 
Query:   87 MTDNIILCELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHVSNEMKSKMEDILSKHSNPNIVPSNLTPESLEDRLCDLALDETDEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWWVFEMVEEIDSKNLLRIPRIFDKINDIKMLSKHTNSIIRYNIMSVLMSYVYICHRYNGDHHSFRDEIVDEIHEICPVLFSNMVFSNLEETTGAFVLRASDAKIQFNNAMXXXXXXXXXXXXRAEETGRTFILSALSDFRLILKSGRAKMRQSYKKVEFYISWLSTSRQFQGFXXXXXXXXXXXXXXSFRLK 1064
            MTDNIILCELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHVSNEMKSKMEDILSKHSNPNIVPSNLTPESLEDRLCDLALDETDEIWNILTS EKEEFFKFIESDEIRKFVKCWKPWWVFEMVEEIDSKNLLRIPRIFDKINDIKMLSKHTNSIIRYNIMSVLMSYVYICHRYNGDHHSF DEIVDEIHEICPVLFSNMVFSNLEETTGAFVLRASDAKIQFNNAMLLLIIDDLFYFLRAEETGRTFILSALSDFRLILKSGRAKMRQSYKKVEFYISWLSTSRQFQGFVEELKNELEVVKMVSFRLK
Sbjct:    1 MTDNIILCELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHVSNEMKSKMEDILSKHSNPNIVPSNLTPESLEDRLCDLALDETDEIWNILTSVEKEEFFKFIESDEIRKFVKCWKPWWVFEMVEEIDSKNLLRIPRIFDKINDIKMLSKHTNSIIRYNIMSVLMSYVYICHRYNGDHHSFHDEIVDEIHEICPVLFSNMVFSNLEETTGAFVLRASDAKIQFNNAMLLLIIDDLFYFLRAEETGRTFILSALSDFRLILKSGRAKMRQSYKKVEFYISWLSTSRQFQGFVEELKNELEVVKMVSFRLK 326
[Close]
BLAST of HIT-type domain-containing protein vs. Planmine SMEST
Match: SMESG000038692.1 (SMESG000038692.1)

HSP 1 Score: 46.9802 bits (110), Expect = 2.067e-6
Identity = 39/110 (35.45%), Postives = 47/110 (42.73%), Query Frame = 3 
Query:  108 CELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCCMSELQGVHVSNEMKSKMEDILSKHSNPNIVPSNLTPESLEDRLCDLA-------LDETDEIWNILTS 416
            C +C   K KYKCP+C I YCSI CYTS K                       N +K+K E  L       I PS LTP S  D   D         LDE+D + +IL +
Sbjct:    3 CSVCKINKKKYKCPKCFIKYCSISCYTSHKGT-------------------CKNRLKTKQETQLV-----FIDPSKLTPLSYGDDNRDYVTHDQLKLLDESDRLKDILAN 88
The following BLAST results are available for this feature:
BLAST of HIT-type domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ZNHIT21.349e-1822.68zinc finger HIT-type containing 2 [Source:HGNC Sym... [more]
ZNHIT25.975e-754.55zinc finger HIT-type containing 2 [Source:HGNC Sym... [more]
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BLAST of HIT-type domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zhit-21.393e-1228.81Zinc finger, HIT-type [Source:UniProtKB/TrEMBL;Ac... [more]
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BLAST of HIT-type domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG312239.608e-2626.71gene:FBgn0051223 transcript:FBtr0475019[more]
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BLAST of HIT-type domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
BX248510.42.127e-2322.50pep chromosome:GRCz11:CHR_ALT_CTG10_2_4:26977650:2... [more]
znhit22.508e-2322.50zinc finger, HIT-type containing 2 [Source:ZFIN;Ac... [more]
BX088530.21.873e-2124.18pep chromosome:GRCz11:CHR_ALT_CTG10_1_25:26947297:... [more]
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BLAST of HIT-type domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
dnajc72.860e-1930.66DnaJ heat shock protein family (Hsp40) member C7 [... [more]
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BLAST of HIT-type domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Znhit21.017e-2021.43zinc finger, HIT domain containing 2 [Source:MGI S... [more]
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BLAST of HIT-type domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|Q2TBW5|ZNHI2_BOVIN1.611e-2121.57Zinc finger HIT domain-containing protein 2 OS=Bos... [more]
sp|Q9QY66|ZNHI2_MOUSE7.111e-2021.43Zinc finger HIT domain-containing protein 2 OS=Mus... [more]
sp|Q9UHR6|ZNHI2_HUMAN6.478e-1822.68Zinc finger HIT domain-containing protein 2 OS=Hom... [more]
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BLAST of HIT-type domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
K1QXW42.049e-3831.46Zinc finger HIT domain-containing protein 2 OS=Cra... [more]
A0A1B6G7501.085e-3633.61HIT-type domain-containing protein (Fragment) OS=C... [more]
A0A1B6J2031.409e-3629.74HIT-type domain-containing protein OS=Homalodisca ... [more]
D2A4C11.788e-3635.19Zinc finger HIT domain-containing protein 2-like P... [more]
A0A1J1HLD92.690e-3630.72CLUMA_CG002561, isoform A OS=Clunio marinus OX=568... [more]
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BLAST of HIT-type domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
znhit22.650e-935.44zinc finger HIT-type containing 2 [Source:NCBI gen... [more]
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BLAST of HIT-type domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of HIT-type domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
HIT12.268e-643.59Protein involved in C/D snoRNP assembly; regulates... [more]
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BLAST of HIT-type domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO421222.011e-1023.11Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of HIT-type domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
znhit29.517e-1137.14zinc finger HIT-type containing 2 [Source:NCBI gen... [more]
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BLAST of HIT-type domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000039467.10.000e+0100.00SMESG000039467.1[more]
SMESG000005538.10.000e+099.39SMESG000005538.1[more]
SMESG000038692.12.067e-635.45SMESG000038692.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015846 ID=SMED30015846|Name=HIT-type domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1152bp
TCAGTTTTAAGTCAATTTTATTCATAATATTTCTAAAAAAGAAATCGAGC
TATAAAAAATTGTTTTAATATTATGTAATTTTATAAATGACTGACAATAT
TATTTTATGTGAATTATGTTCTAAATTGAAGTCTAAATACAAATGCCCAA
GATGTGATATTAATTACTGTTCAATTAAATGCTATACATCAGAAAAGCAT
AATCAATGTTCAGAAAACTTTTATAGGGATTGTTGTATGTCAGAATTACA
GGGAGTTCATGTTTCAAATGAAATGAAATCAAAAATGGAAGATATCTTAT
CAAAACACTCAAATCCTAATATTGTTCCTTCAAATCTCACGCCGGAGTCT
CTGGAAGACAGGTTATGCGATTTAGCTTTAGATGAAACCGATGAAATATG
GAATATATTAACCTCTGACGAAAAAGAAGAATTCTTTAAATTTATAGAAT
CTGATGAAATAAGAAAATTTGTCAAGTGTTGGAAACCATGGTGGGTATTC
GAAATGGTAGAAGAGATAGATAGTAAAAATTTGTTGCGAATCCCTAGAAT
ATTCGACAAAATTAATGATATAAAAATGTTATCTAAACATACTAATAGTA
TAATTAGATATAATATTATGAGTGTTTTAATGTCATATGTTTATATATGT
CACAGGTATAATGGTGATCATCATAGTTTCCGCGACGAAATTGTTGATGA
AATTCATGAAATATGCCCAGTACTGTTTTCGAATATGGTATTTTCAAATT
TAGAAGAAACTACCGGGGCATTTGTTTTAAGGGCTTCAGATGCGAAAATC
CAGTTTAATAATGCTATGCTTTTATTAATTATCGATGATTTATTTTATTT
CCTGCGAGCGGAAGAAACAGGAAGGACATTTATTCTTTCTGCTCTGTCAG
ATTTTCGTTTGATTTTAAAATCTGGTAGAGCAAAAATGAGGCAATCTTAT
AAAAAAGTAGAATTTTATATTAGTTGGCTCTCGACTTCAAGACAATTTCA
AGGTTTTGTTGAGGAATTAAAAAATGAACTAGAAGTTGTAAAAATGGTCA
GCTTTAGATTGAAATAAAGTTTATTGTTTTTTCTTTTTTTATTATATTAT
TTATAATGACAAATCAACTTAGAAACTGTTTGAACCGTTTTTTTAATTGT
AA
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protein sequence of SMED30015846-orf-1

>SMED30015846-orf-1 ID=SMED30015846-orf-1|Name=SMED30015846-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=327bp
MTDNIILCELCSKLKSKYKCPRCDINYCSIKCYTSEKHNQCSENFYRDCC
MSELQGVHVSNEMKSKMEDILSKHSNPNIVPSNLTPESLEDRLCDLALDE
TDEIWNILTSDEKEEFFKFIESDEIRKFVKCWKPWWVFEMVEEIDSKNLL
RIPRIFDKINDIKMLSKHTNSIIRYNIMSVLMSYVYICHRYNGDHHSFRD
EIVDEIHEICPVLFSNMVFSNLEETTGAFVLRASDAKIQFNNAMLLLIID
DLFYFLRAEETGRTFILSALSDFRLILKSGRAKMRQSYKKVEFYISWLST
SRQFQGFVEELKNELEVVKMVSFRLK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR039646ZNHIT2
IPR007529Znf_HIT
Vocabulary: molecular function
TermDefinition
GO:0046872metal ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.30.60.190coord: 2..51
e-value: 2.4E-13
score: 51.5
NoneNo IPR availableSUPERFAMILYSSF144232HIT/MYND zinc finger-likecoord: 5..39
IPR007529Zinc finger, HIT-typePFAMPF04438zf-HITcoord: 7..33
e-value: 7.8E-8
score: 32.1
IPR007529Zinc finger, HIT-typePROSITEPS51083ZF_HITcoord: 8..41
score: 10.626
IPR039646Zinc finger HIT domain-containing protein 2PANTHERPTHR15555ZINC FINGER HIT DOMAIN CONTAINING PROTEIN 2 PROTEIN FON -RELATEDcoord: 5..315