SMED30015809
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30015809 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Alignments
SMED30015809 aligns in the following genomic locations:
Homology
BLAST of SMED30015809 vs. TrEMBL
Match: A0A267FPC5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig015604g2 PE=4 SV=1) HSP 1 Score: 164.081 bits (414), Expect = 3.878e-47 Identity = 73/192 (38.02%), Postives = 116/192 (60.42%), Query Frame = 3 Query: 69 YLISHFKESDAYSYRNSIIRQITILERNFCGREVAFCQKMQPIETNTENKFGIGIIHFPDFQIAKNWYESSHEIRQPDWLNNVTLILVPARNDVDFTMPIVEFAEWNFFRDVQKFQNEYLSHLNTADKGQNNIIAAPEYTYVKLRGNWCPKYLAVTQWASINALQNYADERGPMMLEILNSVAGGNIWAGVN 644 Y + F + AY+Y NS+ R +I++R+ G E AF +Q IE + +G G+++FP+F AK WYE ++E+RQ DWL + +++VPAR D M ++E +W+ FRD F++EYL LN +G+ NI+A P+Y ++R +W PKYL + QW+ + ++Y G M +VA G++ GVN Sbjct: 16 YAVIRFTQDIAYNYHNSVKRTASIIQRSHGGHECAFLPDVQRIEGDERRLYGYGVLYFPNFDAAKYWYECTNEVRQQDWLWSADIVIVPARGAFDKGMAVLEINDWSAFRDQTAFEDEYLKGLNNTTEGRWNIVACPDYLQYRMRSSWSPKYLVINQWSDVETWRSYRQAHGAAMHSFAQTVATGSVQVGVN 207
BLAST of SMED30015809 vs. TrEMBL
Match: A0A267DNX7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig015604g1 PE=4 SV=1) HSP 1 Score: 164.081 bits (414), Expect = 4.139e-47 Identity = 73/192 (38.02%), Postives = 116/192 (60.42%), Query Frame = 3 Query: 69 YLISHFKESDAYSYRNSIIRQITILERNFCGREVAFCQKMQPIETNTENKFGIGIIHFPDFQIAKNWYESSHEIRQPDWLNNVTLILVPARNDVDFTMPIVEFAEWNFFRDVQKFQNEYLSHLNTADKGQNNIIAAPEYTYVKLRGNWCPKYLAVTQWASINALQNYADERGPMMLEILNSVAGGNIWAGVN 644 Y + F + AY+Y NS+ R +I++R+ G E AF +Q IE + +G G+++FP+F AK WYE ++E+RQ DWL + +++VPAR D M ++E +W+ FRD F++EYL LN +G+ NI+A P+Y ++R +W PKYL + QW+ + ++Y G M +VA G++ GVN Sbjct: 16 YAVIRFTQDIAYNYHNSVKRTASIIQRSHGGHECAFLPDVQRIEGDERRLYGYGVLYFPNFDAAKYWYECTNEVRQQDWLWSADIVIVPARGAFDKGMAVLEINDWSAFRDQTAFEDEYLKGLNNTTEGRWNIVACPDYLQYRMRSSWSPKYLVINQWSDVETWRSYRQAHGAAMHSFAQTVATGSVQVGVN 207
BLAST of SMED30015809 vs. TrEMBL
Match: A0A267G5A5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig002947g3 PE=4 SV=1) HSP 1 Score: 161.77 bits (408), Expect = 4.201e-46 Identity = 79/207 (38.16%), Postives = 119/207 (57.49%), Query Frame = 3 Query: 33 MAEYYDFPNGE---CYLISHFKESDAYSYRNSIIRQITILERNFCGREVAFCQKMQPIETNTENKFGIGIIHFPDFQIAKNWYESSHEIRQPDWLNNVTLILVPARNDVDFTMPIVEFAEWNFFRDVQKFQNEYLSHLNTADKGQNNIIAAPEYTYVKLRGNWCPKYLAVTQWASINALQNYADERGPMMLEILNSVAGGNIWAGVN 644 MA Y+ E Y + F + AY+Y NSI R +I++R+ G++ AF +Q IE ++G G+++FP F+ AK WYE ++E+RQ DWL + ++LVPAR D M +VE +W+ FRD F+NEYL+ LN G+ N++A P+Y +LR +W PKYL + QW + A + Y G + + A G + GVN Sbjct: 1 MASYHGLKEPENVGYYAVIRFGKDIAYNYHNSIKRIASIIQRSHNGQDCAFLPDVQLIEGEDRRQYGYGLLYFPSFEAAKYWYECTNEVRQQDWLWSAAILLVPARGAFDKAMSVVEINDWSDFRDQTAFENEYLAELNDPSDGRWNVVACPDYLQYRLRSSWSPKYLVINQWPDVEAWRRYRQANGVRVHHFALTAATGTVAVGVN 207
BLAST of SMED30015809 vs. TrEMBL
Match: A0A267DBP7 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig002947g1 PE=4 SV=1) HSP 1 Score: 161.384 bits (407), Expect = 6.585e-46 Identity = 79/207 (38.16%), Postives = 119/207 (57.49%), Query Frame = 3 Query: 33 MAEYYDFPNGE---CYLISHFKESDAYSYRNSIIRQITILERNFCGREVAFCQKMQPIETNTENKFGIGIIHFPDFQIAKNWYESSHEIRQPDWLNNVTLILVPARNDVDFTMPIVEFAEWNFFRDVQKFQNEYLSHLNTADKGQNNIIAAPEYTYVKLRGNWCPKYLAVTQWASINALQNYADERGPMMLEILNSVAGGNIWAGVN 644 MA Y+ E Y + F + AY+Y NSI R +I++R+ G++ AF +Q IE ++G G+++FP F+ AK WYE ++E+RQ DWL + ++LVPAR D M +VE +W+ FRD F+NEYL+ LN G+ N++A P+Y +LR +W PKYL + QW + A + Y G + + A G + GVN Sbjct: 10 MASYHGLKEPENVGYYAVIRFGKDIAYNYHNSIKRIASIIQRSHNGQDCAFLPDVQLIEGEDRRQYGYGLLYFPSFEAAKYWYECTNEVRQQDWLWSAAILLVPARGAFDKAMSVVEINDWSDFRDQTAFENEYLAELNDPSDGRWNVVACPDYLQYRLRSSWSPKYLVINQWPDVEAWRRYRQANGVRVHHFALTAATGTVAVGVN 216
BLAST of SMED30015809 vs. TrEMBL
Match: A0A1I8FH46 (XendoU domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 115.161 bits (287), Expect = 7.291e-26 Identity = 51/129 (39.53%), Postives = 80/129 (62.02%), Query Frame = 3 Query: 69 YLISHFKESDAYSYRNSIIRQITILERNFCGREVAFCQKMQPIETNTENKFGIGIIHFPDFQIAKNWYESSHEIRQPDWLNNVTLILVPARNDVDFTMPIVEFAEWNFFRDVQKFQNEYLSHLNTADKG 455 Y + F + AY+Y NS+ R +I++R+ G E AF +Q IE + +G G+++FP+F AK WYE ++E+RQ DWL + +++VPAR D M ++E +W+ FRD F++EYL LN +G Sbjct: 436 YAVIRFTQDIAYNYHNSVKRTASIIQRSHGGHECAFLPDVQRIEGDERRLYGYGVLYFPNFDAAKYWYECTNEVRQQDWLWSADIVIVPARGAFDKGMAVLEINDWSAFRDQTAFEDEYLKGLNNTTEG 564
BLAST of SMED30015809 vs. Planmine SMEST
Match: SMESG000069776.1 (SMESG000069776.1) HSP 1 Score: 436.802 bits (1122), Expect = 2.406e-158 Identity = 207/208 (99.52%), Postives = 208/208 (100.00%), Query Frame = 3 Query: 33 MAEYYDFPNGECYLISHFKESDAYSYRNSIIRQITILERNFCGREVAFCQKMQPIETNTENKFGIGIIHFPDFQIAKNWYESSHEIRQPDWLNNVTLILVPARNDVDFTMPIVEFAEWNFFRDVQKFQNEYLSHLNTADKGQNNIIAAPEYTYVKLRGNWCPKYLAVTQWASINALQNYADERGPMMLEILNSVAGGNIWAGVNSKSK 656 MAEYYDFPNGECYLISHFKESDAYSYRNSI+RQITILERNFCGREVAFCQKMQPIETNTENKFGIGIIHFPDFQIAKNWYESSHEIRQPDWLNNVTLILVPARNDVDFTMPIVEFAEWNFFRDVQKFQNEYLSHLNTADKGQNNIIAAPEYTYVKLRGNWCPKYLAVTQWASINALQNYADERGPMMLEILNSVAGGNIWAGVNSKSK Sbjct: 1 MAEYYDFPNGECYLISHFKESDAYSYRNSIVRQITILERNFCGREVAFCQKMQPIETNTENKFGIGIIHFPDFQIAKNWYESSHEIRQPDWLNNVTLILVPARNDVDFTMPIVEFAEWNFFRDVQKFQNEYLSHLNTADKGQNNIIAAPEYTYVKLRGNWCPKYLAVTQWASINALQNYADERGPMMLEILNSVAGGNIWAGVNSKSK 208
BLAST of SMED30015809 vs. Planmine SMEST
Match: SMESG000007291.1 (SMESG000007291.1) HSP 1 Score: 71.2478 bits (173), Expect = 7.300e-15 Identity = 59/185 (31.89%), Postives = 92/185 (49.73%), Query Frame = 3 Query: 63 ECYLISHFKESDAYSYRNSIIRQITILER---NFCGREVAFCQKMQPIETNTENKFGIGIIHFPDFQIAKNWYESSHEIRQPDWLNNVTLILVPARNDVDFT------MPIVEFAEWNFFRDVQKFQNEYL--SHLNTADKGQNNIIAAPEYTYVKLRGNWCPKYLAVTQWASINALQNY--ADE 578 EC++ F +S A +YRN+ +++ ++ R + G+ V F +Q IE N K +G+ F + AK W ES +E RQ D+L NV + + P N+ D T M I+ E FF+D+ F ++YL +T + G + + E Y RG P + V Q+ SI + Y ADE Sbjct: 21 ECFVFILFPKSRAQAYRNAAVKEKAVISRYSIHKPGQIVGFAYHVQAIEGNWNCKLSVGVFRFATYNDAKMWLESDNEFRQQDFLTNVDIWIAPIINE-DLTRKGYAIMQIIS-QEVCFFKDI--FLHKYLPNEEASTINHGGHPFVINHENPYC-WRGVPPPGHTIVNQFPSIMNFERYLAADE 200
BLAST of SMED30015809 vs. Planmine SMEST
Match: SMESG000062647.1 (SMESG000062647.1) HSP 1 Score: 63.929 bits (154), Expect = 4.650e-12 Identity = 48/178 (26.97%), Postives = 89/178 (50.00%), Query Frame = 3 Query: 63 ECYLISHFKESDAYSYRNSIIRQITILER---NFCGREVAFCQKMQPIETNTENKFGIGIIHFPDFQIAKNWYESSHEIRQPDWLNNVTLILVPA-RNDVDFT-MPIVEFAEWNF-FRDVQKFQNEYLSHLN---TADKGQNNIIAAPEYTYVKLRGNWCPKYLAVTQWASINALQNY 569 EC+++ F ES +YRN+ +++ ++ R + G+ V F +++ IE + + IG + F ++ A W ES + RQ D+L +V + +VP R D+ PI++ + D+ F ++YL T+ +G ++ P T RG P ++ + Q+ S +A NY Sbjct: 53 ECFVLIIFPESRGQAYRNAAVKEKAVISRYSIHAPGKNVGFAYQVKVIEGYWDTQLSIGALKFASYRDASMWLESDNAFRQQDFLTHVDIWIVPINREDMSHKGYPILQMVSQKVCYPDI--FFHKYLPAEEAAVTSHEGVPFVVNHP--TPYAWRGKKPPGHIVINQFPSCDAFNNY 226
BLAST of SMED30015809 vs. Planmine SMEST
Match: SMESG000008612.1 (SMESG000008612.1) HSP 1 Score: 61.2326 bits (147), Expect = 4.935e-12 Identity = 34/93 (36.56%), Postives = 54/93 (58.06%), Query Frame = 3 Query: 279 SHEIRQPDWLNNVTLILVPAR---NDVDFTMPIVEFAEWNFFRDVQKFQNEYLSHLNTADKGQNNIIAAPEYTYVKLRGNWCPKYLAVTQWAS 548 S+E+RQ +WL V +++VP+R +D IVE E +R +F +E L +++A +NNI+A P Y LRG+W P Y+ + W+S Sbjct: 2 SYEVRQKEWLFPVDIVIVPSRWRIKSIDENT-IVEILEIVKYRSPSRFSSECLDKIDSA--KENNIVAVPRYATEVLRGSWNPLYIIINVWSS 91
BLAST of SMED30015809 vs. Planmine SMEST
Match: SMESG000004187.1 (SMESG000004187.1) HSP 1 Score: 54.6842 bits (130), Expect = 6.137e-9 Identity = 40/177 (22.60%), Postives = 84/177 (47.46%), Query Frame = 3 Query: 60 GECYLISHFKESDAYSYRNSIIRQITILERNFCGREVAFCQKMQPIETNTENKFGIGIIHFPDFQIAKNWYESSHEIRQPDWLNNVTLILVPARNDVDFTMPIVEFAEWNFFR----DVQKFQNEYLSHLNTADKG---QNNIIAAPEYTYVKLRGNWCPKYLAVTQWASINALQNY 569 GE ++ F + S+ N+++++ I+ G+ V F ++++ IE + K+ IG++ F + AK W S +Q DWL+ V + ++P P E+ F + ++F +++L +A G +N I + + R N+C ++ ++ W S++ N+ Sbjct: 20 GEVLVLILFDATLTKSFCNTMVKEKNIIRNYGNGQMVGFSEEIKVIEGDWPQKYTIGLLRFDTMKDAKKWNFSDPLTKQQDWLDQVEIYVMPLLK----AFPKKEYQVMELFNCDLINEEQFYHKFLPSYKSAIDGNGCENFIACTDKVERWRGRHNFC--HVMISLWPSLDDYMNW 190 The following BLAST results are available for this feature:
BLAST of SMED30015809 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30015809 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30015809 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30015809 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30015809 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30015809 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30015809 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30015809 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30015809 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30015809 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30015809 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30015809 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30015809 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30015809 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30015809 ID=SMED30015809|Name=SMED30015809|organism=Schmidtea mediterranea sexual|type=transcript|length=680bpback to top protein sequence of SMED30015809-orf-1 >SMED30015809-orf-1 ID=SMED30015809-orf-1|Name=SMED30015809-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=209bp MAEYYDFPNGECYLISHFKESDAYSYRNSIIRQITILERNFCGREVAFCQback to top Annotated Terms
The following terms have been associated with this transcript:
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