RNA pseudouridine synthase domain containing 1

Overview
NameRNA pseudouridine synthase domain containing 1
Smed IDSMED30015760
Length (bp)1051
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of RNA pseudouridine synthase domain containing 1 (SMED30015760) t-SNE clustered cells

Violin plots show distribution of expression levels for RNA pseudouridine synthase domain containing 1 (SMED30015760) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of RNA pseudouridine synthase domain containing 1 (SMED30015760) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for RNA pseudouridine synthase domain containing 1 (SMED30015760) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30015760

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30015760SMESG000021497.1 SmedASXL_011939SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30015760SMESG000021497.1 SmedASXL_011939SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Human
Match: RPUSD1 (RNA pseudouridine synthase domain containing 1 [Source:HGNC Symbol;Acc:HGNC:14173])

HSP 1 Score: 164.851 bits (416), Expect = 1.140e-47
Identity = 100/252 (39.68%), Postives = 131/252 (51.98%), Query Frame = 3 
Query:  240 YEDINILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYGTYP-YEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKWQSMLTFHS 992
            Y   + L+V+K  D+ I+     ++ LTL  QL Y     AD +T + FRFCHQLD+STSG +C+  NKA  G   + F++R   K YLAL+ GH+  +              IS  IG      ST    +  C      C  P+ + T+++VLEHGLY     SKVLL PLTGRTHQLRVHC  LGH ++GD  YG     E +  RMM HA  L +P+    E + V + DPF   LD  W       S
Sbjct:   13 YRSRDFLVVNKHWDVRID-SKAWRETLTLQKQLRYRFPELADPDTCYGFRFCHQLDFSTSGALCVALNKAAAGSAYRCFKERRVTKAYLALLRGHIQESRVT-----------ISHAIGRN----STEGRAHTMCIEGSQGCENPKPSLTDLVVLEHGLYAGDPVSKVLLKPLTGRTHQLRVHCSALGHPVVGDLTYGEVSGREDRPFRMMLHAFYLRIPTDT--ECVEVCTPDPFLPSLDACWSPHTLLQS 246
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Human
Match: RPUSD1 (RNA pseudouridine synthase domain containing 1 [Source:HGNC Symbol;Acc:HGNC:14173])

HSP 1 Score: 164.851 bits (416), Expect = 1.140e-47
Identity = 100/252 (39.68%), Postives = 131/252 (51.98%), Query Frame = 3 
Query:  240 YEDINILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYGTYP-YEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKWQSMLTFHS 992
            Y   + L+V+K  D+ I+     ++ LTL  QL Y     AD +T + FRFCHQLD+STSG +C+  NKA  G   + F++R   K YLAL+ GH+  +              IS  IG      ST    +  C      C  P+ + T+++VLEHGLY     SKVLL PLTGRTHQLRVHC  LGH ++GD  YG     E +  RMM HA  L +P+    E + V + DPF   LD  W       S
Sbjct:   13 YRSRDFLVVNKHWDVRID-SKAWRETLTLQKQLRYRFPELADPDTCYGFRFCHQLDFSTSGALCVALNKAAAGSAYRCFKERRVTKAYLALLRGHIQESRVT-----------ISHAIGRN----STEGRAHTMCIEGSQGCENPKPSLTDLVVLEHGLYAGDPVSKVLLKPLTGRTHQLRVHCSALGHPVVGDLTYGEVSGREDRPFRMMLHAFYLRIPTDT--ECVEVCTPDPFLPSLDACWSPHTLLQS 246
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Human
Match: RPUSD1 (RNA pseudouridine synthase domain containing 1 [Source:HGNC Symbol;Acc:HGNC:14173])

HSP 1 Score: 98.9821 bits (245), Expect = 2.153e-24
Identity = 61/169 (36.09%), Postives = 89/169 (52.66%), Query Frame = 3 
Query:  240 YEDINILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVL 746
            Y   + L+V+K  D+ I+     ++ LTL  QL Y     AD +T + FRFCHQLD+STSG +C+  NKA  G   + F++R   K YLAL+ GH+  +              IS  IG +  T    ++  I+ +     C  P+ + T+++VLEHGLY     SKVL
Sbjct:   13 YRSRDFLVVNKHWDVRID-SKAWRETLTLQKQLRYRFPELADPDTCYGFRFCHQLDFSTSGALCVALNKAAAGSAYRCFKERRVTKAYLALLRGHIQESRVT-----------ISHAIG-RNSTEGRAHTMCIEGSQGVAGCENPKPSLTDLVVLEHGLYAGDPVSKVL 168
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Human
Match: RPUSD1 (RNA pseudouridine synthase domain containing 1 [Source:HGNC Symbol;Acc:HGNC:14173])

HSP 1 Score: 97.8265 bits (242), Expect = 6.861e-24
Identity = 51/105 (48.57%), Postives = 63/105 (60.00%), Query Frame = 3 
Query:  663 CIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYGTYP-YEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKWQS 974
            C  P+ + T+++VLEHGLY     SKVLL PLTGRTHQLRVHC  LGH ++GD  YG     E +  RMM HA  L +P+    E + V + DPF   LD  W  
Sbjct:    9 CENPKPSLTDLVVLEHGLYAGDPVSKVLLKPLTGRTHQLRVHCSALGHPVVGDLTYGEVSGREDRPFRMMLHAFYLRIPTDT--ECVEVCTPDPFLPSLDACWSP 111
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Human
Match: RPUSD1 (RNA pseudouridine synthase domain containing 1 [Source:HGNC Symbol;Acc:HGNC:14173])

HSP 1 Score: 98.2117 bits (243), Expect = 7.730e-24
Identity = 51/105 (48.57%), Postives = 63/105 (60.00%), Query Frame = 3 
Query:  663 CIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYGTYP-YEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKWQS 974
            C  P+ + T+++VLEHGLY     SKVLL PLTGRTHQLRVHC  LGH ++GD  YG     E +  RMM HA  L +P+    E + V + DPF   LD  W  
Sbjct:   12 CENPKPSLTDLVVLEHGLYAGDPVSKVLLKPLTGRTHQLRVHCSALGHPVVGDLTYGEVSGREDRPFRMMLHAFYLRIPTDT--ECVEVCTPDPFLPSLDACWSP 114
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Zebrafish
Match: rpusd1 (RNA pseudouridine synthase domain containing 1 [Source:NCBI gene;Acc:767786])

HSP 1 Score: 160.614 bits (405), Expect = 2.071e-46
Identity = 101/248 (40.73%), Postives = 133/248 (53.63%), Query Frame = 3 
Query:  240 YEDINILIVSKSHDLMINHGN-RNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYG----TYPYEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKW 968
            Y+  N ++V+K  D+ I+      KQ  T+ +QL +     AD  TY+ FRFCHQLD+STSG +C+  NKA  GQ  + F+ R   K YLALV G +   N +     G    + +TE  T           ++ CT     C  P+   TE+ VLE+G YD    +KVLL PLTGRTHQLRVHC  +GH I+GD  Y       PY     RMM HA+ L++P     E I+V + DPF   LD KW
Sbjct:   13 YQSTNYIVVNKHWDIRIDSKMWYEKQ--TVQSQLKHRFPELADPGTYYGFRFCHQLDFSTSGALCVALNKAAAGQAYRCFKDRRVTKAYLALVRGTVTEENLSLDFAIG----KNTTEGKT-----------HMMCTEGTEGCENPKPCQTEVTVLEYGTYDGDPVTKVLLQPLTGRTHQLRVHCSAIGHPIVGDYTYSLRTDNSPY-----RMMLHAYFLHIPLHN--EPIHVTAPDPFVPSLDAKW 236
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Zebrafish
Match: rpusd1 (RNA pseudouridine synthase domain containing 1 [Source:NCBI gene;Acc:767786])

HSP 1 Score: 160.614 bits (405), Expect = 2.071e-46
Identity = 101/248 (40.73%), Postives = 133/248 (53.63%), Query Frame = 3 
Query:  240 YEDINILIVSKSHDLMINHGN-RNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYG----TYPYEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKW 968
            Y+  N ++V+K  D+ I+      KQ  T+ +QL +     AD  TY+ FRFCHQLD+STSG +C+  NKA  GQ  + F+ R   K YLALV G +   N +     G    + +TE  T           ++ CT     C  P+   TE+ VLE+G YD    +KVLL PLTGRTHQLRVHC  +GH I+GD  Y       PY     RMM HA+ L++P     E I+V + DPF   LD KW
Sbjct:   13 YQSTNYIVVNKHWDIRIDSKMWYEKQ--TVQSQLKHRFPELADPGTYYGFRFCHQLDFSTSGALCVALNKAAAGQAYRCFKDRRVTKAYLALVRGTVTEENLSLDFAIG----KNTTEGKT-----------HMMCTEGTEGCENPKPCQTEVTVLEYGTYDGDPVTKVLLQPLTGRTHQLRVHCSAIGHPIVGDYTYSLRTDNSPY-----RMMLHAYFLHIPLHN--EPIHVTAPDPFVPSLDAKW 236
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Xenopus
Match: RPUSD1 (RNA pseudouridine synthase domain containing 1 [Source:NCBI gene;Acc:100380010])

HSP 1 Score: 157.147 bits (396), Expect = 5.792e-45
Identity = 99/254 (38.98%), Postives = 136/254 (53.54%), Query Frame = 3 
Query:  240 YEDINILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECY-RISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYGTYPYEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKWQSMLTFHSCS 998
            Y+  + L+V+K  D+ I+     ++ LT+ +QL +     AD  TY+ FRFCHQLD+STSG +C+  NK   G+  + F+ RT  K YLALV G ++            E Y RIS  IG       T    ++ C    P C   +   T+++VL+HG Y     +KVLL PLTGRTHQLRVHC  LG  I+GD  Y ++  + +  RMM HA+ L +P+    E I V + DPFQ   D  W    T  S S
Sbjct:   13 YQSADFLVVNKHWDIRIDSKMWYEK-LTVQSQLKHRFPELADPGTYYGFRFCHQLDFSTSGALCVALNKEAAGRAYQCFKDRTVTKAYLALVRGTVS------------EKYMRISLAIGKNTQEGLT----HMMCVEGAPGCKNAKPCQTDLVVLQHGSYKGDPVTKVLLQPLTGRTHQLRVHCSALGFPIVGDFTY-SFKKDSEPYRMMLHAYYLRIPTD--HELIEVTAPDPFQPDTDPNWTPHDTLCSLS 246
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Xenopus
Match: RPUSD2 (RNA pseudouridylate synthase domain containing 2 [Source:NCBI gene;Acc:100497757])

HSP 1 Score: 50.8322 bits (120), Expect = 1.887e-6
Identity = 41/147 (27.89%), Postives = 64/147 (43.54%), Query Frame = 3 
Query:  396 HQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYGT 836
            H+LD  TSGV+          ++ +   +R  +K+Y+  V G         ++T     + +S +IG               C +D     K +   T    L +    N  +S V   P TGRTHQ+RVH ++LGH I+ D +Y T
Sbjct:  218 HRLDRMTSGVLMFAKTLEVSKKIDEQVRQRQLEKEYVCRVCGEFP----KEEVTCEEPIFVVSYKIGV--------------CRVDP----KGKPCKTVFQRLSY----NGKSSVVKCFPYTGRTHQIRVHLQYLGHPIVNDPIYNT 338
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Mouse
Match: Rpusd1 (RNA pseudouridylate synthase domain containing 1 [Source:MGI Symbol;Acc:MGI:1919186])

HSP 1 Score: 167.933 bits (424), Expect = 4.409e-49
Identity = 103/244 (42.21%), Postives = 132/244 (54.10%), Query Frame = 3 
Query:  240 YEDINILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYG-TYPYEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKW 968
            Y+  + L+V+K  DL I+     ++ LTL  QL ++    AD +T + FRFCHQLD+STSG +C+  NKA  G   K F++R   K YLALV GH+       ++T      R STE  T           +  C      C  P+ + TE++VLEHGLY     SKVLL PLTGRTHQLRVHC  LGH I+GD  YG     E Q  RMM HA  L +P+    E +   + DPF   LD  W
Sbjct:   13 YQSSDFLVVNKHWDLRID-SKTWRETLTLQKQLRHHFPELADPDTCYGFRFCHQLDFSTSGALCVALNKAAAGSAYKCFKERRVTKAYLALVRGHVQ----ESQVTINYAIGRNSTEGRT-----------HTMCIEGTHGCENPKPSLTELLVLEHGLYAGDPVSKVLLKPLTGRTHQLRVHCSALGHPIVGDLTYGQAEDQEDQPFRMMLHAFYLRIPTQA--ERVEACTPDPFLPALDACW 238
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Mouse
Match: Rpusd1 (RNA pseudouridylate synthase domain containing 1 [Source:MGI Symbol;Acc:MGI:1919186])

HSP 1 Score: 167.933 bits (424), Expect = 4.409e-49
Identity = 103/244 (42.21%), Postives = 132/244 (54.10%), Query Frame = 3 
Query:  240 YEDINILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYG-TYPYEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKW 968
            Y+  + L+V+K  DL I+     ++ LTL  QL ++    AD +T + FRFCHQLD+STSG +C+  NKA  G   K F++R   K YLALV GH+       ++T      R STE  T           +  C      C  P+ + TE++VLEHGLY     SKVLL PLTGRTHQLRVHC  LGH I+GD  YG     E Q  RMM HA  L +P+    E +   + DPF   LD  W
Sbjct:   13 YQSSDFLVVNKHWDLRID-SKTWRETLTLQKQLRHHFPELADPDTCYGFRFCHQLDFSTSGALCVALNKAAAGSAYKCFKERRVTKAYLALVRGHVQ----ESQVTINYAIGRNSTEGRT-----------HTMCIEGTHGCENPKPSLTELLVLEHGLYAGDPVSKVLLKPLTGRTHQLRVHCSALGHPIVGDLTYGQAEDQEDQPFRMMLHAFYLRIPTQA--ERVEACTPDPFLPALDACW 238
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Mouse
Match: Rpusd1 (RNA pseudouridylate synthase domain containing 1 [Source:MGI Symbol;Acc:MGI:1919186])

HSP 1 Score: 101.293 bits (251), Expect = 2.788e-25
Identity = 53/103 (51.46%), Postives = 62/103 (60.19%), Query Frame = 3 
Query:  663 CIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYG-TYPYEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKW 968
            C  P+ + TE++VLEHGLY     SKVLL PLTGRTHQLRVHC  LGH I+GD  YG     E Q  RMM HA  L +P+    E +   + DPF   LD  W
Sbjct:    9 CENPKPSLTELLVLEHGLYAGDPVSKVLLKPLTGRTHQLRVHCSALGHPIVGDLTYGQAEDQEDQPFRMMLHAFYLRIPTQA--ERVEACTPDPFLPALDACW 109
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Mouse
Match: Rpusd2 (RNA pseudouridylate synthase domain containing 2 [Source:MGI Symbol;Acc:MGI:1918066])

HSP 1 Score: 49.2914 bits (116), Expect = 5.376e-6
Identity = 41/147 (27.89%), Postives = 61/147 (41.50%), Query Frame = 3 
Query:  396 HQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYGT 836
            H+LD  TSGV+      A   ++ +    R  +K+Y+  V G                      E+  K+      Y   + C +D      P+    E +     L  N  +S V   PLTGRTHQ+RVH + LGH I+ D +Y +
Sbjct:  284 HRLDRLTSGVLMFAKTAAVSEKIHEQVRDRQLEKEYVCRVAGEFP-----------------DKEVICKEPILVVSYKVGV-CRVD------PRGKPCETVF--QRLSYNGRSSVVQCRPLTGRTHQIRVHLQFLGHPILNDPIYNS 404
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. UniProt/SwissProt
Match: sp|Q17QT4|RUSD1_BOVIN (RNA pseudouridylate synthase domain-containing protein 1 OS=Bos taurus OX=9913 GN=RPUSD1 PE=2 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 3.263e-49
Identity = 103/248 (41.53%), Postives = 134/248 (54.03%), Query Frame = 3 
Query:  252 NILIVSKSHDLMINHGNRN--------KQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYG-TYPYEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKW 968
            N+ +V +SHD ++ + + +        ++ LTL  QL +     AD +TY+ FRFCHQLD+STSG +C+  NKA  G   + F+ R   K YLALV GH+       +MT       IS  IG      ST    +  C      C  P+ + TE++VLEHGLY     SKVLL PLTGRTHQLRVHC  LGH I+GD  YG     E Q  RMM HA  L +P++   E +   + DPF   LD  W
Sbjct:    8 NLCVVYRSHDFLVVNKHWDVRIDSKAWRETLTLQKQLRHRFPELADPDTYYGFRFCHQLDFSTSGALCVALNKAAAGSAYRCFKDRRVTKAYLALVRGHVQ----ESRMT-------ISYAIGKN----STEGRTHTMCIEGTQGCENPKPSLTELVVLEHGLYAGDPVSKVLLQPLTGRTHQLRVHCSALGHPIVGDLTYGHALGQEDQPFRMMLHAFYLRIPTSA--ECVEACTPDPFVPSLDACW 238
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. UniProt/SwissProt
Match: sp|Q8VCZ8|RUSD1_MOUSE (RNA pseudouridylate synthase domain-containing protein 1 OS=Mus musculus OX=10090 GN=Rpusd1 PE=2 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 3.084e-48
Identity = 103/244 (42.21%), Postives = 132/244 (54.10%), Query Frame = 3 
Query:  240 YEDINILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYG-TYPYEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKW 968
            Y+  + L+V+K  DL I+     ++ LTL  QL ++    AD +T + FRFCHQLD+STSG +C+  NKA  G   K F++R   K YLALV GH+       ++T      R STE  T           +  C      C  P+ + TE++VLEHGLY     SKVLL PLTGRTHQLRVHC  LGH I+GD  YG     E Q  RMM HA  L +P+    E +   + DPF   LD  W
Sbjct:   13 YQSSDFLVVNKHWDLRID-SKTWRETLTLQKQLRHHFPELADPDTCYGFRFCHQLDFSTSGALCVALNKAAAGSAYKCFKERRVTKAYLALVRGHVQ----ESQVTINYAIGRNSTEGRT-----------HTMCIEGTHGCENPKPSLTELLVLEHGLYAGDPVSKVLLKPLTGRTHQLRVHCSALGHPIVGDLTYGQAEDQEDQPFRMMLHAFYLRIPTQA--ERVEACTPDPFLPALDACW 238
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. UniProt/SwissProt
Match: sp|Q9UJJ7|RUSD1_HUMAN (RNA pseudouridylate synthase domain-containing protein 1 OS=Homo sapiens OX=9606 GN=RPUSD1 PE=1 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 5.477e-47
Identity = 100/252 (39.68%), Postives = 131/252 (51.98%), Query Frame = 3 
Query:  240 YEDINILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYGTYP-YEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKWQSMLTFHS 992
            Y   + L+V+K  D+ I+     ++ LTL  QL Y     AD +T + FRFCHQLD+STSG +C+  NKA  G   + F++R   K YLAL+ GH+  +              IS  IG      ST    +  C      C  P+ + T+++VLEHGLY     SKVLL PLTGRTHQLRVHC  LGH ++GD  YG     E +  RMM HA  L +P+    E + V + DPF   LD  W       S
Sbjct:   13 YRSRDFLVVNKHWDVRID-SKAWRETLTLQKQLRYRFPELADPDTCYGFRFCHQLDFSTSGALCVALNKAAAGSAYRCFKERRVTKAYLALLRGHIQESRVT-----------ISHAIGRN----STEGRAHTMCIEGSQGCENPKPSLTDLVVLEHGLYAGDPVSKVLLKPLTGRTHQLRVHCSALGHPVVGDLTYGEVSGREDRPFRMMLHAFYLRIPTDT--ECVEVCTPDPFLPSLDACWSPHTLLQS 246
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. UniProt/SwissProt
Match: sp|Q08C69|RUSD1_DANRE (RNA pseudouridylate synthase domain-containing protein 1 OS=Danio rerio OX=7955 GN=rpusd1 PE=2 SV=1)

HSP 1 Score: 160.614 bits (405), Expect = 1.199e-45
Identity = 101/251 (40.24%), Postives = 135/251 (53.78%), Query Frame = 3 
Query:  240 YEDINILIVSKSHDLMINHGN-RNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYG----TYPYEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKWQSM 977
            Y+  N ++V+K  D+ I+      KQ  T+ +QL ++    AD  TY+ FRFCHQLD+STSG +C+  NKA  GQ  + F+ R   K YLALV G +   N +     G    + +TE  T           ++ CT     C  P+   TE+ VLE+G YD    +KVLL PLTGRTHQLRVHC  +GH I+GD  Y       PY     RMM HA+ L++P     E I+V + DPF   LD KW  +
Sbjct:   13 YQSTNYIVVNKHWDIRIDSKMWYEKQ--TVQSQLKHHFPELADPGTYYGFRFCHQLDFSTSGALCVALNKAAAGQAYRCFKDRRVTKAYLALVRGTVTEENLSLDFAIG----KNTTEGKT-----------HMMCTEGTEGCENPKPCQTEVTVLEYGTYDGDQVTKVLLQPLTGRTHQLRVHCSAIGHPIVGDYTYSLRTDNSPY-----RMMLHAYFLHIPLHN--EPIHVTAPDPFVPSLDAKWAPL 239
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. UniProt/SwissProt
Match: sp|P0AA38|RLUA_SHIFL (Dual-specificity RNA pseudouridine synthase RluA OS=Shigella flexneri OX=623 GN=rluA PE=3 SV=2)

HSP 1 Score: 94.7449 bits (234), Expect = 1.581e-21
Identity = 66/221 (29.86%), Postives = 103/221 (46.61%), Query Frame = 3 
Query:  240 YEDINILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTK---QVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYGTYPYEKQFKRMMFHAHKLNL 893
            Y+D +I++V+K   L+   G   +   ++  ++  +         Y      H+LD +TSGVI +   KA   ++ + F +R  KKQY+A VWGH +          GL    +  +   +   +V Y TG                 + A TE  V+E+   DN A  +V+L P+TGR+HQLRVH   LGH I+GD  Y +        R++ HA  L +
Sbjct:   18 YQDDHIMVVNKPSGLLSVPGRLEEHKDSVMTRIQRD---------YPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWGHPS-------PAEGLVDLPLICDWPNRPKQKVCYETG-----------------KPAQTEYEVVEYAA-DNTA--RVVLKPITGRSHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTI 202
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. TrEMBL
Match: R7UFN6 (PseudoU_synth_2 domain-containing protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_224650 PE=4 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 4.429e-53
Identity = 99/262 (37.79%), Postives = 148/262 (56.49%), Query Frame = 3 
Query:  240 YEDINILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYGTYPYEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKWQSMLTFHSCSDYSYCSVTQ 1025
            Y+  N ++V+K +DL+IN  + +   L++  QL +      D    H FRF H+LDYSTSGV+C+G N+   G    +F  R A K Y AL+ GH++            +  ++  +IG        G   +  C   +P C   +SA+TE+++L+HG Y+ +  SKVLLIP TGRTHQLRVHC  LGH+I+GD  Y        F RMM HAH+L LP +K  E+++V + DPFQ   D +W+   +F +  ++ +C  T+
Sbjct:   48 YQSSNFIVVNKHYDLVIN--SDDPSVLSVETQLRHLHPELVDSSIAHGFRFAHRLDYSTSGVLCLGLNRKAAGWAMNAFRNRRAVKYYTALLRGHVSPTQAKN------QSRKLEGDIGMDVAALERG--AHHMCLASEPNCKDAKSATTELVLLQHGQYEGRPVSKVLLIPHTGRTHQLRVHCAALGHHIVGDYTYSRRTDTNTF-RMMLHAHRLCLPLSK--EDLDVTAPDPFQPEFDARWKPEQSFMTVQEWEHCEATE 296
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. TrEMBL
Match: D2HDX7 (RNA pseudouridine synthase domain containing 1 (Fragment) OS=Ailuropoda melanoleuca OX=9646 GN=RPUSD1 PE=4 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 3.193e-48
Identity = 104/244 (42.62%), Postives = 136/244 (55.74%), Query Frame = 3 
Query:  240 YEDINILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYGTYP-YEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKW 968
            Y+  + L+V+K  DL I+     ++  TL  QL ++    AD +TY+ FRFCHQLD+STSG +C+  NKA  G   K F++R   K YLALV GH+  N    +MT      R STE           ++  I+ T+    C  P+ + TE++VLEHGLY     SKVLL PLTGRTHQLRVHC  L H ++GD  YG  P  E Q  RMM HA  L +P+    E +   + DPF   LD  W
Sbjct:   13 YQSPDFLVVNKHWDLRID-SKMWRETPTLQKQLRHHFPELADPDTYYGFRFCHQLDFSTSGALCVALNKAAAGSAYKCFKERRVTKAYLALVRGHVQEN----RMTINYAIGRNSTE--------GRAHTMCIEGTLG---CENPKPSLTELVVLEHGLYAGDPVSKVLLQPLTGRTHQLRVHCSALSHPVVGDLTYGQAPDQEDQPFRMMLHAFYLRIPTHT--ERVEACTPDPFVPALDACW 238
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. TrEMBL
Match: A0A091DWF4 (RNA pseudouridylate synthase domain-containing protein 1 OS=Fukomys damarensis OX=885580 GN=H920_03786 PE=4 SV=1)

HSP 1 Score: 171.785 bits (434), Expect = 2.555e-47
Identity = 105/244 (43.03%), Postives = 135/244 (55.33%), Query Frame = 3 
Query:  240 YEDINILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYG-TYPYEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKW 968
            Y+  + L+V+K  DL I+     ++ LTL  QL ++    AD +T + FRFCHQLD+STSG +C+  NKA  G   + F++R   K YLALV GH+       ++T      R STE  T           +  C      C  P+ + TE++VLEHGLYD    SKVLL PLTGRTHQLRVHC  LGH I+GD  YG     E Q  RMM HA  L +P+  L E +   + DPF S LD  W
Sbjct:   13 YQSRDFLVVNKHWDLRID-CKTWRETLTLQKQLRHHFPELADPDTCYGFRFCHQLDFSTSGALCVALNKAAAGSAYRCFKERRVTKAYLALVRGHIQ----ESQLTVSYAIGRNSTEGRT-----------HTMCIEGSQGCENPKPSLTELLVLEHGLYDGNPVSKVLLKPLTGRTHQLRVHCSALGHPIVGDLTYGQATGQEDQPFRMMLHAFYLRIPT--LAECVEACTPDPFLSTLDACW 238
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. TrEMBL
Match: H9GEK8 (RNA pseudouridine synthase domain containing 1 OS=Anolis carolinensis OX=28377 GN=RPUSD1 PE=4 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 3.028e-47
Identity = 103/254 (40.55%), Postives = 135/254 (53.15%), Query Frame = 3 
Query:  240 YEDINILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYGTYPYEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKWQSMLTFHSCSD 1001
            Y+  + ++V+K  D+ I+     ++ LTL +QL +     AD +TY+ FRFCHQLD+STSG +C+  NKA  G   K F++R   K YLALV GH+       K+T       I   IG       T    ++ C      C  P+   +E+ VLEHGLY     SKVLL PLTGRTHQLRVHC  +GH I+GD  Y        F RMM HA+ L +P+ K  E + V + DPF   LD  W    T H   D
Sbjct:   13 YQSADFIVVNKHWDVRIDSKMWYEK-LTLQSQLKHRFPELADPDTYYGFRFCHQLDFSTSGALCVALNKAAAGSAYKCFKERMVTKAYLALVRGHVQ----EAKLT-------IEYAIGKNTTEGMT----HMMCIEGTEGCENPKPCQSELTVLEHGLYSGDPVSKVLLQPLTGRTHQLRVHCSAVGHPIVGDFTYSLKTDGGPF-RMMLHAYYLRIPTAK--ELVEVSAPDPFVPALDSHWMPQQTVHRLDD 247
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. TrEMBL
Match: G5BXU8 (RNA pseudouridylate synthase domain-containing protein 1 OS=Heterocephalus glaber OX=10181 GN=GW7_12965 PE=4 SV=1)

HSP 1 Score: 171.785 bits (434), Expect = 3.062e-47
Identity = 105/244 (43.03%), Postives = 135/244 (55.33%), Query Frame = 3 
Query:  240 YEDINILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYG-TYPYEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKW 968
            Y+  + L+V+K  DL I+     ++ LTL  QL ++    AD +T + FRFCHQLD+STSG +C+  NKA  G   + F++R   K YLALV GH+       ++T      R STE  T           +  C      C  P+ + TE++VLEHGLYD    SKVLL PLTGRTHQLRVHC  LGH I+GD  YG     E Q  RMM HA  L +P+  L E +   + DPF S LD  W
Sbjct:   13 YQSRDFLVVNKHWDLRID-CKTWRETLTLQKQLRHHFPELADPDTCYGFRFCHQLDFSTSGALCVALNKAAAGSAYRCFKERRVTKAYLALVRGHIQ----ESQLTVSYAIGRNSTEGRT-----------HTMCIEGTQGCENPKPSLTELLVLEHGLYDGNPVSKVLLKPLTGRTHQLRVHCSALGHPIVGDLTYGQATGQEDQPFRMMLHAFYLRIPT--LAECVEACTPDPFLSTLDACW 238
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Cavefish
Match: rpusd1 (RNA pseudouridine synthase domain containing 1 [Source:NCBI gene;Acc:103042140])

HSP 1 Score: 150.599 bits (379), Expect = 1.166e-42
Identity = 98/258 (37.98%), Postives = 133/258 (51.55%), Query Frame = 3 
Query:  240 YEDINILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYG----TYPYEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKW--QSMLTFHSC 995
            Y   N L+V+K  D+ I+     ++  T+  QL ++    AD  TY+ FRFCHQLD+STSG +C+  NKA  G+    F+ R   K YLALV G +       K+       + STE  T           ++ C  +   C  P+ + TE+ VLE+G+YD    +KVLL P TGRTHQLRVHC  +GH I+GD  Y       PY     RMM HA+ L +P     E + V + DPF    D KW  Q++L    C
Sbjct:   13 YHSENYLVVNKHWDIRIDSKFWAEE-RTVQKQLKHHFPDLADPATYYGFRFCHQLDFSTSGALCVALNKAAAGRAYHFFKNRLVTKAYLALVRGSVP----EEKLMIDFAIGKNSTEGKT-----------HMMCVEETEGCENPKPSQTELNVLEYGVYDGDPVTKVLLQPRTGRTHQLRVHCSAIGHPIVGDYTYSLGSDNSPY-----RMMLHAYMLQIPLE--HEPLQVTAPDPFLPTTDPKWSPQTVLLTVDC 247
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Sea Lamprey
Match: rpusd1 (RNA pseudouridine synthase domain containing 1 [Source:NCBI gene;Acc:767786])

HSP 1 Score: 147.517 bits (371), Expect = 4.307e-42
Identity = 97/273 (35.53%), Postives = 136/273 (49.82%), Query Frame = 3 
Query:  243 EDINILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYF-RFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYG----TYPYEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKWQSMLTFHSCSDYSYCSVTQ*IAQNLR 1046
            ED + L++ K  DL I+   R ++ LT+  QL +      D  T H F RFCHQLDY+T G +C+  ++   G   ++F +R   K YLALV GH+   +    MT       I   IG       T    ++ C    P C  P+   TE+ VL+ G Y +  A+KVLL P+TGRTHQLRVHC  +GH I+GD  Y     T PY     R M HA  L +P  +    +  E+ DPF   +D  W        C + +  SV + +A+ +R
Sbjct:   14 EDADYLVLDKHWDLRID-STRPRETLTVQQQLRHRYPQLLDPSTRHGFSRFCHQLDYAT-GALCVALSRQAAGGAFRAFSQRRVVKAYLALVRGHVKEEH----MT-------IDYAIGKDTREGQT----HMMCVEATPGCDNPKPCLTELTVLQTGRYGDDPATKVLLQPVTGRTHQLRVHCDAVGHAIVGDYTYSLRADTAPY-----RTMLHAALLRMPLGE--RTVQAEAPDPFVPDVDPSW--------CPEQTLRSVDEALAEVVR 254
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Yeast
Match: PUS6 (tRNA:pseudouridine synthase; catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability [Source:SGD;Acc:S000003401])

HSP 1 Score: 62.3882 bits (150), Expect = 2.269e-11
Identity = 43/151 (28.48%), Postives = 70/151 (46.36%), Query Frame = 3 
Query:  393 CHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWG-----HLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMY 830
            C++LD  TSG++ +  N    G++ KS   R   K YLA V G      L L+N N   T   +  +++ E+      YS    +     +        + A T+   + +  + +   + V   P+TGRTHQ+R+H   LGH I+ D +Y
Sbjct:  164 CYRLDKITSGLLILAKNSQSAGEIQKSIRSRDMIKIYLARVKGRFPHSELILDNENAAETTFEDTSKVTVEMTP---IYSIDPKRQFPVGLS-----TSKDAITKFYPIRY--FSHADETVVACKPITGRTHQIRIHLARLGHPIVNDSVY 304
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Medaka
Match: rpusd1 (RNA pseudouridine synthase domain containing 1 [Source:NCBI gene;Acc:101166304])

HSP 1 Score: 156.377 bits (394), Expect = 7.576e-45
Identity = 98/243 (40.33%), Postives = 135/243 (55.56%), Query Frame = 3 
Query:  252 NILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMY--GT--YPYEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKW 968
            + L++ K  D+ I+     ++ LT+  QL ++    AD  TY+ FRFCHQLD+STSG +C+  NKA  G+  + F+ RT  K YLALV G +       + + G    + STE G K +    G             C  P+ + T++ VLE+GLYD    +KVLL PLTGRTHQLRVHC  +GH+I+GD  Y  GT   PY     RMM HAH L++P     + + V + DPF   +D KW
Sbjct:   20 DFLVLDKHWDIRIDSKMWYEK-LTVQAQLQHHFPDLADPSTYYGFRFCHQLDFSTSGALCVALNKAAAGRAYRCFKDRTVTKAYLALVRGTVEETTQTIRFSIG----KNSTE-GKKHMMLIEGSEG----------CENPKPSETKLTVLEYGLYDGDPVTKVLLQPLTGRTHQLRVHCSAIGHSIVGDFTYSLGTDNSPY-----RMMLHAHLLHIPLEP--DPLLVTAGDPFVPTVDPKW 239
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Medaka
Match: rpusd1 (RNA pseudouridine synthase domain containing 1 [Source:NCBI gene;Acc:101166304])

HSP 1 Score: 156.377 bits (394), Expect = 7.576e-45
Identity = 98/243 (40.33%), Postives = 135/243 (55.56%), Query Frame = 3 
Query:  252 NILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMY--GT--YPYEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKW 968
            + L++ K  D+ I+     ++ LT+  QL ++    AD  TY+ FRFCHQLD+STSG +C+  NKA  G+  + F+ RT  K YLALV G +       + + G    + STE G K +    G             C  P+ + T++ VLE+GLYD    +KVLL PLTGRTHQLRVHC  +GH+I+GD  Y  GT   PY     RMM HAH L++P     + + V + DPF   +D KW
Sbjct:   20 DFLVLDKHWDIRIDSKMWYEK-LTVQAQLQHHFPDLADPSTYYGFRFCHQLDFSTSGALCVALNKAAAGRAYRCFKDRTVTKAYLALVRGTVEETTQTIRFSIG----KNSTE-GKKHMMLIEGSEG----------CENPKPSETKLTVLEYGLYDGDPVTKVLLQPLTGRTHQLRVHCSAIGHSIVGDFTYSLGTDNSPY-----RMMLHAHLLHIPLEP--DPLLVTAGDPFVPTVDPKW 239
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. Planmine SMEST
Match: SMESG000021497.1 (SMESG000021497.1)

HSP 1 Score: 608.986 bits (1569), Expect = 0.000e+0
Identity = 308/311 (99.04%), Postives = 309/311 (99.36%), Query Frame = 3 
Query:   93 KSLFNSIKNEVLTQSFRIYIKFRWDLQMIYHLFXXXXXXXXXXXXXXXXYEDINILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYGTYPYEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKWQSMLTFHSCSDYSYCSVTQ 1025
            KSLFNSIKNEVLTQSFRIYIKFRWDLQMIYHLFLKRRNNIININNLKILYEDINILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCN KMTNGLECYRI+TEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYGTYPYEKQFKRMM HAHKLNLPSTKLFEEINVESEDPFQSHLDDKWQSMLTFHSCSDYSYCSVTQ
Sbjct:    4 KSLFNSIKNEVLTQSFRIYIKFRWDLQMIYHLFLKRRNNIININNLKILYEDINILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHYFRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLNNCNSKMTNGLECYRINTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYGTYPYEKQFKRMMLHAHKLNLPSTKLFEEINVESEDPFQSHLDDKWQSMLTFHSCSDYSYCSVTQ 314
[Close]
BLAST of RNA pseudouridine synthase domain containing 1 vs. Planmine SMEST
Match: SMESG000051935.1 (SMESG000051935.1)

HSP 1 Score: 60.4622 bits (145), Expect = 6.610e-10
Identity = 49/167 (29.34%), Postives = 72/167 (43.11%), Query Frame = 3 
Query:  384 FRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNLN-----NCNRKMTNGLECYRI------STEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSASTEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYGTYPYEK 851
             R  H++D  TSG++  G +     ++S     R   K YLA+V G  N        CN+ +  G+ C ++      S E G K                          AST+ M L++   + +  S VL  PLTGRTHQ+RVH ++LG  I  D +Y    + K
Sbjct:  181 LRVIHRIDRMTSGILLFGKDYETSSKLSGQIRDRQVLKYYLAVVGGKFNEEKEQPIECNKPL--GILCPKVGLAYLKSVEEGGK-------------------------IASTKFMFLKYN--EKQNLSLVLCKPLTGRTHQIRVHLQYLGFPISNDPLYNCISWGK 318
The following BLAST results are available for this feature:
BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RPUSD11.140e-4739.68RNA pseudouridine synthase domain containing 1 [So... [more]
RPUSD11.140e-4739.68RNA pseudouridine synthase domain containing 1 [So... [more]
RPUSD12.153e-2436.09RNA pseudouridine synthase domain containing 1 [So... [more]
RPUSD16.861e-2448.57RNA pseudouridine synthase domain containing 1 [So... [more]
RPUSD17.730e-2448.57RNA pseudouridine synthase domain containing 1 [So... [more]
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BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rpusd12.071e-4640.73RNA pseudouridine synthase domain containing 1 [So... [more]
rpusd12.071e-4640.73RNA pseudouridine synthase domain containing 1 [So... [more]
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BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
RPUSD15.792e-4538.98RNA pseudouridine synthase domain containing 1 [So... [more]
RPUSD21.887e-627.89RNA pseudouridylate synthase domain containing 2 [... [more]
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BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Rpusd14.409e-4942.21RNA pseudouridylate synthase domain containing 1 [... [more]
Rpusd14.409e-4942.21RNA pseudouridylate synthase domain containing 1 [... [more]
Rpusd12.788e-2551.46RNA pseudouridylate synthase domain containing 1 [... [more]
Rpusd25.376e-627.89RNA pseudouridylate synthase domain containing 2 [... [more]
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BLAST of RNA pseudouridine synthase domain containing 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q17QT4|RUSD1_BOVIN3.263e-4941.53RNA pseudouridylate synthase domain-containing pro... [more]
sp|Q8VCZ8|RUSD1_MOUSE3.084e-4842.21RNA pseudouridylate synthase domain-containing pro... [more]
sp|Q9UJJ7|RUSD1_HUMAN5.477e-4739.68RNA pseudouridylate synthase domain-containing pro... [more]
sp|Q08C69|RUSD1_DANRE1.199e-4540.24RNA pseudouridylate synthase domain-containing pro... [more]
sp|P0AA38|RLUA_SHIFL1.581e-2129.86Dual-specificity RNA pseudouridine synthase RluA O... [more]
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BLAST of RNA pseudouridine synthase domain containing 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
R7UFN64.429e-5337.79PseudoU_synth_2 domain-containing protein OS=Capit... [more]
D2HDX73.193e-4842.62RNA pseudouridine synthase domain containing 1 (Fr... [more]
A0A091DWF42.555e-4743.03RNA pseudouridylate synthase domain-containing pro... [more]
H9GEK83.028e-4740.55RNA pseudouridine synthase domain containing 1 OS=... [more]
G5BXU83.062e-4743.03RNA pseudouridylate synthase domain-containing pro... [more]
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BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpusd11.166e-4237.98RNA pseudouridine synthase domain containing 1 [So... [more]
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BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpusd14.307e-4235.53RNA pseudouridine synthase domain containing 1 [So... [more]
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BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
PUS62.269e-1128.48tRNA:pseudouridine synthase; catalyzes the convers... [more]
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BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNA pseudouridine synthase domain containing 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rpusd17.576e-4540.33RNA pseudouridine synthase domain containing 1 [So... [more]
rpusd17.576e-4540.33RNA pseudouridine synthase domain containing 1 [So... [more]
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BLAST of RNA pseudouridine synthase domain containing 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000021497.10.000e+099.04SMESG000021497.1[more]
SMESG000051935.16.610e-1029.34SMESG000051935.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015760 ID=SMED30015760|Name=RNA pseudouridine synthase domain containing 1|organism=Schmidtea mediterranea sexual|type=transcript|length=1051bp
GTTTAAATATTTATCTTTAAATTTGGCCAATAAACGAATTTAATATTGAA
TTACTTTTATATCTTTCATTACTTTTTAATTCGATGTCACTAAAATCCTT
ATTTAATTCTATTAAAAATGAGGTTTTAACTCAAAGTTTTAGAATATATA
TAAAATTTCGATGGGATCTTCAAATGATTTATCACTTGTTTTTAAAAAGA
AGAAATAATATAATCAATATTAATAACTTGAAAATTCTTTATGAAGATAT
AAATATTTTAATAGTATCTAAATCTCACGATTTGATGATTAATCACGGAA
ATCGCAACAAACAGTTCCTAACTCTTAGCAATCAATTGTATTATAATAGA
TATTGGAAAGCAGATTTTGAAACATATCATTATTTTCGATTTTGTCATCA
ATTGGACTATTCTACTAGTGGAGTAATATGTATTGGTTACAATAAAGCGG
GATGTGGACAAGTTTCGAAAAGTTTCGAAAAAAGAACCGCCAAGAAACAA
TATTTAGCTTTAGTATGGGGGCATTTGAATTTGAACAATTGCAACCGTAA
GATGACAAATGGACTAGAGTGCTATCGCATAAGTACTGAAATCGGCACGA
AACAAGTAACGTATTCTACTGGTTACAGTCAATATATTCAATGCACAATT
GATGATCCTTTATGTATTAAACCCCAATCAGCATCGACGGAGATAATGGT
GCTTGAGCATGGATTATATGATAATAAAGCTGCCTCTAAAGTGCTACTGA
TTCCTCTCACAGGGAGAACTCATCAGTTGCGAGTGCATTGCAAGCATCTG
GGCCACAACATCATCGGGGATTGCATGTATGGCACATATCCATATGAGAA
GCAATTCAAGCGAATGATGTTCCACGCTCACAAACTGAATTTGCCGAGTA
CAAAATTATTCGAAGAAATAAATGTCGAATCCGAAGATCCATTCCAAAGC
CATTTGGACGATAAATGGCAGTCGATGTTGACTTTCCACAGCTGCAGTGA
TTACAGTTATTGTTCTGTTACTCAATAAATTGCTCAAAATCTGAGATCGG
A
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protein sequence of SMED30015760-orf-1

>SMED30015760-orf-1 ID=SMED30015760-orf-1|Name=SMED30015760-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=315bp
MSLKSLFNSIKNEVLTQSFRIYIKFRWDLQMIYHLFLKRRNNIININNLK
ILYEDINILIVSKSHDLMINHGNRNKQFLTLSNQLYYNRYWKADFETYHY
FRFCHQLDYSTSGVICIGYNKAGCGQVSKSFEKRTAKKQYLALVWGHLNL
NNCNRKMTNGLECYRISTEIGTKQVTYSTGYSQYIQCTIDDPLCIKPQSA
STEIMVLEHGLYDNKAASKVLLIPLTGRTHQLRVHCKHLGHNIIGDCMYG
TYPYEKQFKRMMFHAHKLNLPSTKLFEEINVESEDPFQSHLDDKWQSMLT
FHSCSDYSYCSVTQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR006145PsdUridine_synth_RsuA/RluA
IPR020103PsdUridine_synth_cat_dom_sf
Vocabulary: molecular function
TermDefinition
GO:0003723RNA binding
GO:0009982pseudouridine synthase activity
Vocabulary: biological process
TermDefinition
GO:0001522pseudouridine synthesis
GO:0009451RNA modification
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006145Pseudouridine synthase, RsuA/RluAPFAMPF00849PseudoU_synth_2coord: 58..238
e-value: 7.2E-21
score: 74.9
IPR006145Pseudouridine synthase, RsuA/RluACDDcd02869PseudoU_synth_RluCD_likecoord: 58..268
e-value: 2.89937E-40
score: 137.084
NoneNo IPR availableGENE3DG3DSA:3.30.2350.10coord: 44..285
e-value: 5.9E-45
score: 155.4
NoneNo IPR availablePANTHERPTHR21600RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE Bcoord: 48..295
NoneNo IPR availablePANTHERPTHR21600:SF41RNA PSEUDOURIDYLATE SYNTHASE DOMAIN-CONTAINING PROTEIN 1coord: 48..295
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILYSSF55120Pseudouridine synthasecoord: 44..285