SMED30015755

Overview
NameSMED30015755
Smed IDSMED30015755
Length (bp)1133
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30015755 (SMED30015755) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30015755 (SMED30015755) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30015755 (SMED30015755) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30015755 (SMED30015755) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Homology
BLAST of SMED30015755 vs. Planmine SMEST
Match: SMESG000039477.1 (SMESG000039477.1)

HSP 1 Score: 264.233 bits (674), Expect = 7.169e-86
Identity = 154/158 (97.47%), Postives = 156/158 (98.73%), Query Frame = -1
Query:  624 MEPTKIIEKLKGXXXXXXXXXXXXXNKQKLIKRAKEDISTISNLYSDEHCXXXXXXXXXXDVTTERIYEILSMRLEITKFGKSWNKLMKKVENIEDEIATTMKEQTKAYVECYACKTEEARVMVIPCGHLSYCHGCAQESHCMLCQRKIKERLTVYFP 1097
            MEPTKIIEKLKGILNIENDNLEELINKQKLIKRAKEDISTISNLYSDEHCILIKL DIDKD+TTERIYEILSMRLEITKFGKSWNKLMKK ENIEDEIATTMKEQTKAY+ECYACKTEEARVMVIPCGHLSYCHGCAQESHCMLCQRKIKERLTVYFP
Sbjct:  186 MEPTKIIEKLKGILNIENDNLEELINKQKLIKRAKEDISTISNLYSDEHCILIKLSDIDKDITTERIYEILSMRLEITKFGKSWNKLMKKAENIEDEIATTMKEQTKAYLECYACKTEEARVMVIPCGHLSYCHGCAQESHCMLCQRKIKERLTVYFP 343          
BLAST of SMED30015755 vs. Planmine SMEST
Match: SMESG000065358.1 (SMESG000065358.1)

HSP 1 Score: 265.388 bits (677), Expect = 2.692e-84
Identity = 155/158 (98.10%), Postives = 157/158 (99.37%), Query Frame = -1
Query:  624 MEPTKIIEKLKGXXXXXXXXXXXXXNKQKLIKRAKEDISTISNLYSDEHCXXXXXXXXXXDVTTERIYEILSMRLEITKFGKSWNKLMKKVENIEDEIATTMKEQTKAYVECYACKTEEARVMVIPCGHLSYCHGCAQESHCMLCQRKIKERLTVYFP 1097
            MEPTKIIEKLKGILNIENDNLEELINKQKLIKRAKEDISTISNLYSDEHCILIKLIDIDKDVTTERIYEIL MRLEITKFGKSWNKLM+KV+NIEDEIATTMKEQTKAYVECYACKTEEARVMVIPCGHLSYCHGCAQESHCMLCQRKIKERLTVYFP
Sbjct:  343 MEPTKIIEKLKGILNIENDNLEELINKQKLIKRAKEDISTISNLYSDEHCILIKLIDIDKDVTTERIYEILFMRLEITKFGKSWNKLMEKVKNIEDEIATTMKEQTKAYVECYACKTEEARVMVIPCGHLSYCHGCAQESHCMLCQRKIKERLTVYFP 500          
BLAST of SMED30015755 vs. Planmine SMEST
Match: SMESG000029555.1 (SMESG000029555.1)

HSP 1 Score: 202.986 bits (515), Expect = 1.431e-57
Identity = 108/115 (93.91%), Postives = 112/115 (97.39%), Query Frame = -1
Query:  675 KQKLIKRAKEDISTISNLYSDEHCXXXXXXXXXXDVTTERIYEILSMRLEITKFGKSWNKLMKKVENIEDEIATTMKEQTKAYVECYACKTEEARVMVIPCGHLSYCHGCAQESH 1019
            KQKLIKRAKEDIS ISNLYSDEHCILIKLIDIDKDVTTERIYEILSMR+EITKFGKSW KLM+KV+NIEDEIATT+KEQTKAYVECY CKTEEARVMVIPCGHLSYCHGCAQESH
Sbjct:  556 KQKLIKRAKEDISAISNLYSDEHCILIKLIDIDKDVTTERIYEILSMRVEITKFGKSWYKLMEKVKNIEDEIATTVKEQTKAYVECYTCKTEEARVMVIPCGHLSYCHGCAQESH 670          
BLAST of SMED30015755 vs. Planmine SMEST
Match: SMESG000029555.1 (SMESG000029555.1)

HSP 1 Score: 202.986 bits (515), Expect = 1.472e-57
Identity = 108/115 (93.91%), Postives = 112/115 (97.39%), Query Frame = -1
Query:  675 KQKLIKRAKEDISTISNLYSDEHCXXXXXXXXXXDVTTERIYEILSMRLEITKFGKSWNKLMKKVENIEDEIATTMKEQTKAYVECYACKTEEARVMVIPCGHLSYCHGCAQESH 1019
            KQKLIKRAKEDIS ISNLYSDEHCILIKLIDIDKDVTTERIYEILSMR+EITKFGKSW KLM+KV+NIEDEIATT+KEQTKAYVECY CKTEEARVMVIPCGHLSYCHGCAQESH
Sbjct:  549 KQKLIKRAKEDISAISNLYSDEHCILIKLIDIDKDVTTERIYEILSMRVEITKFGKSWYKLMEKVKNIEDEIATTVKEQTKAYVECYTCKTEEARVMVIPCGHLSYCHGCAQESH 663          
BLAST of SMED30015755 vs. Planmine SMEST
Match: SMESG000052862.1 (SMESG000052862.1)

HSP 1 Score: 194.126 bits (492), Expect = 1.624e-54
Identity = 116/159 (72.96%), Postives = 138/159 (86.79%), Query Frame = -1
Query:  624 MEPTK-IIEKLKGXXXXXXXXXXXXXNKQKLIKRAKEDISTISNLYSDEHCXXXXXXXXXXDVTTERIYEILSMRLEITKFGKSWNKLMKKVENIEDEIATTMKEQTKAYVECYACKTEEARVMVIPCGHLSYCHGCAQESHCMLCQRKIKERLTVYFP 1097
            ME TK II KL+ ILNIENDNLE LINKQKL+++ KED+++IS+LY DE C LIKLI+ID++V TERIYEIL MRLEI +FGK W+ +M+KVE IEDEI   MKEQTKAY ECY C+TEEARVMVIPCGHLS+C GCA+ESHC+LCQRKIKER+TV++P
Sbjct: 2359 MEQTKPIITKLREILNIENDNLERLINKQKLMRKVKEDLASISSLYPDELCTLIKLINIDEEVITERIYEILLMRLEIAEFGKEWSIMMEKVETIEDEIMRMMKEQTKAYRECYVCRTEEARVMVIPCGHLSFCQGCAKESHCVLCQRKIKERITVFYP 2517          
The following BLAST results are available for this feature:
BLAST of SMED30015755 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015755 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015755 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015755 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015755 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015755 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015755 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015755 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015755 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015755 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015755 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015755 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015755 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015755 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000039477.17.169e-8697.47SMESG000039477.1[more]
SMESG000065358.12.692e-8498.10SMESG000065358.1[more]
SMESG000029555.11.431e-5793.91SMESG000029555.1[more]
SMESG000029555.11.472e-5793.91SMESG000029555.1[more]
SMESG000052862.11.624e-5472.96SMESG000052862.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015755 ID=SMED30015755|Name=SMED30015755|organism=Schmidtea mediterranea sexual|type=transcript|length=1133bp
ACCCGTGGCACGACAGTCCGTAGCTGTCGTCTTGTTCCTCACCACCAGCT
AGGGAAAAAAGAAGTCATCTCTAGGTACCTTTAATCAAAACTATTAATTC
TGTTCCGTGTACACTCTTTCTCTCACCTCTCGGTCTATCTCTTCACTCCC
CCCTTCAAAATTTGTGGTAATCGCATCAACAAGCTCCCCAAAAGAGGTGA
TCAATCTCTCGCCGGTTACTTGCTGACATACGCGACTTGGCCTACCAGTT
CCTTTCTCAGTAGGGTTGATCAGGGTTTCCCTTGGGTGAGCGTACTATCA
TTAGATAGCTCAGGTTAACCATTTTTCCTCGTCCTATCTCAATATAATAT
CAATAAATATAATATCAATAAAACAAAAAGAATCATACACTAGGAAACTC
GGTTGTCGAAATTTTTCCGTCCAATATTTGTGTCTCTGTGCATGTGTTTT
AATTATTTCCCTAATTCGGCTTTCATGTATTCGGTCTATTACCTTTATTT
ATCGTTGTCGGGTGGTTTTATTATTTATAAACATTGCATATTTCTTTTAT
ATTCGTCTTATCATCTAATAATAATTCAAGTAATGCATAATACACATTTA
TTTCATTTCATCATAACAATTCATGGAAAGTAAACCGTTAGTCTTTCCTT
TATCTTTCGCTGGCATAACATACAGTGCGACTCTTGTGCGCATCCATGAC
AATAACTCAGGTGTCCGCAAGGTATCACCATTACTCGTGCTTCCTCTGTT
TTGCACGCATAACATTCCACATAAGCCTTGGTTTGCTCTTTCATTGTGGT
TGCGATCTCGTCTTCAATGTTTTCGACCTTTTTCATTAACTTGTTCCAGC
TTTTCCCAAATTTGGTTATCTCTAATCTCATAGATAGAATTTCATATATT
CTTTCAGTTGTTACATCTTTATCAATATCGATCAATTTTATTAATATGCA
ATGTTCGTCGGAGTACAGGTTTGAAATCGTTGATATATCCTCTTTAGCTC
GTTTAATTAACTTTTGTTTATTAATTAGTTCCTCAAGATTGTCATTTTCG
ATGTTTAGAATCCCCTTTAGTTTTTCTATAATTTTTGTTGGTTCCATCTA
AAATTATCAAATTACCCTTTAAATTTATCTAAA
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protein sequence of SMED30015755-orf-1

>SMED30015755-orf-1 ID=SMED30015755-orf-1|Name=SMED30015755-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=159bp
MEPTKIIEKLKGILNIENDNLEELINKQKLIKRAKEDISTISNLYSDEHC
ILIKLIDIDKDVTTERIYEILSMRLEITKFGKSWNKLMKKVENIEDEIAT
TMKEQTKAYVECYACKTEEARVMVIPCGHLSYCHGCAQESHCMLCQRKIK
ERLTVYFP*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000074oocyte
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000231vitelline gland
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR001841Znf_RING
IPR013083Znf_RING/FYVE/PHD
Vocabulary: molecular function
TermDefinition
GO:0008270zinc ion binding
GO:0005515protein binding