SMED30015747
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppSub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30015747 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Alignments
SMED30015747 aligns in the following genomic locations:
Homology
BLAST of SMED30015747 vs. Planmine SMEST
Match: SMESG000040960.1 (SMESG000040960.1) HSP 1 Score: 868.996 bits (2244), Expect = 0.000e+0 Identity = 500/500 (100.00%), Postives = 500/500 (100.00%), Query Frame = 2 Query: 53 MINYFNNIEIKTKTILEKXXXXXXXXXXXXXXVTILGLIHQYFVKILSISYYWNSMIFSGSVMNIVSASIIILNEYLNHLNRNHFYKLRVLNSEILHTFCFPLVSFIIGSLLISTINDIYLIRCIGYGRMIFASTNSIYYITVMIFLAKYHFVCDDGEEWXXXXXXXXXXXXXXXXXXXXXXXXXSVEQITLIVPILNITDFDTSCIQHLNTISKTIDNLTGRLLMPMMKAWLIFGTLCCTLLIMPFLHHYSSLNNHKIEQSIIKNSSFKGVKHDISNFSLIVTLAFGIGFLSCKFFFYYYHTYWTMXXXXXXXXXXXXXXXXXXXYATSEAATWPVCSVKLFQFNNAIVWIGLIGQFVFSVTMIMNSISHLSTEFSILGLCVLFEYLSKMIQISVQFILESLLLETQWENRKLEKNSKLINAFLFLMINNLCLTILNIARINDNILPPLKDPISFKIFFIFTTNFSMVFCVLQRLHGFLLFVRYLIFIANQKLVLGEKQ 1552 MINYFNNIEIKTKTILEKLIFLIGLFLISFGSVTILGLIHQYFVKILSISYYWNSMIFSGSVMNIVSASIIILNEYLNHLNRNHFYKLRVLNSEILHTFCFPLVSFIIGSLLISTINDIYLIRCIGYGRMIFASTNSIYYITVMIFLAKYHFVCDDGEEWIINFSNMSAIFIIIISQIFYILVSISVEQITLIVPILNITDFDTSCIQHLNTISKTIDNLTGRLLMPMMKAWLIFGTLCCTLLIMPFLHHYSSLNNHKIEQSIIKNSSFKGVKHDISNFSLIVTLAFGIGFLSCKFFFYYYHTYWTMILSVILEINISIILLICSLYATSEAATWPVCSVKLFQFNNAIVWIGLIGQFVFSVTMIMNSISHLSTEFSILGLCVLFEYLSKMIQISVQFILESLLLETQWENRKLEKNSKLINAFLFLMINNLCLTILNIARINDNILPPLKDPISFKIFFIFTTNFSMVFCVLQRLHGFLLFVRYLIFIANQKLVLGEKQ Sbjct: 1 MINYFNNIEIKTKTILEKLIFLIGLFLISFGSVTILGLIHQYFVKILSISYYWNSMIFSGSVMNIVSASIIILNEYLNHLNRNHFYKLRVLNSEILHTFCFPLVSFIIGSLLISTINDIYLIRCIGYGRMIFASTNSIYYITVMIFLAKYHFVCDDGEEWIINFSNMSAIFIIIISQIFYILVSISVEQITLIVPILNITDFDTSCIQHLNTISKTIDNLTGRLLMPMMKAWLIFGTLCCTLLIMPFLHHYSSLNNHKIEQSIIKNSSFKGVKHDISNFSLIVTLAFGIGFLSCKFFFYYYHTYWTMILSVILEINISIILLICSLYATSEAATWPVCSVKLFQFNNAIVWIGLIGQFVFSVTMIMNSISHLSTEFSILGLCVLFEYLSKMIQISVQFILESLLLETQWENRKLEKNSKLINAFLFLMINNLCLTILNIARINDNILPPLKDPISFKIFFIFTTNFSMVFCVLQRLHGFLLFVRYLIFIANQKLVLGEKQ 500
BLAST of SMED30015747 vs. Planmine SMEST
Match: SMESG000051898.1 (SMESG000051898.1) HSP 1 Score: 129.413 bits (324), Expect = 4.347e-32 Identity = 118/457 (25.82%), Postives = 223/457 (48.80%), Query Frame = 2 Query: 197 ISYYWNSMIFSGSVMNIVSASIIILNEYLNHLNRNHFYKLRVLNSEILHTFCFPLVSFIIGSLLISTINDIYLIRCIGYGRMIFASTNSIYYITVMIFLAKYHFVCDDGEEWXXXXXXXXXXXXXXXXXXXXXXXXXSVEQITLIVPILNITDFDTSCIQHLNTISKTIDNLTGRLLMPMMKAWLIFGTLCCTLLIMPFLHHYSSLNNHKIEQSIIKNSSFKGVKHDISNFS-----------LIVTLAFGIGFLSCKFFFYYYHTYWTMXXXXXXXXXXXXXXXXXXXYATSEAATWPVCSVKLFQFNNAIVWIGLIGQFVFSVTMIMNSISHLSTEFSILGLCVLFEYLSKMIQISVQFILESLLLETQWENRKLEKNSKLINAFLFLMINNLCLTILNIARINDNILPPLKDPISFKIFFIFTTNFSMVFCVLQRLHGFLLFVRYLIFIANQKL 1534 + + W + GSV N++ +I+ Y+NH RN F K + N +IL+ FC P F+I +L+I+ + +Y+ C G ++F T+ IY++ +++F+A+ + V D GE W +NF NM II+ S +F I+ + S+EQ+ ++N + + SCI +L I++ L + + + K+ ++FG + +Y L +K + K + K D++ S + TL FGI FL+ Y + + + +E+ I ++ +++ +++ FN I+W+GL GQ V +I I + E + + + +L +Y+ +++ VQ + E++L+E W N+ + S + ++ +NN ++ + L P + + K+F NF +F VLQR+HGF + V Y F+ K+ Sbjct: 45 LEFVWAPLYICGSVFNLLVVFLILFALYMNHKYRNLFNKCPMENQDILYYFCIPATIFVISTLIITLVKSLYIAECNGKMIVLFIFTSVIYFVVIVVFIAETNSVFDFGESWALNFVNMMVSLIIVSSLVFNIVFNTSLEQMDYFAAVINKSLINQSCIDNLPFIAQRTYYLWVSVRIFLYKSIVLFGLFSTVFIFKSLYRNYLYLQFYKYDG---KTNDLKSNDEDVNRNSQKNNLKKLYLKIFSTLLFGILFLAINIAVYIRSKEYLEHILIGMEMFSYISMIGLTIFMMKNLIFTDFSISRIYVFNEVIIWLGLCGQLVHCFAVIAAYIYLMYNEVNAIYIAILIKYVLQILSNIVQSVFEAILVEKLWNNKIGKNFSTMAFGAFYIAVNNFVYIFTDLILVYKEFLLPFEGSVKLKLFVCLFFNFGNIFSVLQRIHGFSIHVSYFTFLYRNKI 498
BLAST of SMED30015747 vs. Planmine SMEST
Match: SMESG000040945.1 (SMESG000040945.1) HSP 1 Score: 70.8626 bits (172), Expect = 7.839e-13 Identity = 103/428 (24.07%), Postives = 203/428 (47.43%), Query Frame = 2 Query: 230 GSVMNIVSASIIILNEYLNHLNRNHFYKLRVLNSEILHTFCFPLVSFIIGSLLISTINDIYLIRCIGYGRMIFASTNSIYYITVMIFLAKYHFVCDDGEEWXXXXXXXXXXXXXXXXXXXXXXXXXSVEQITLIVPILNITDFDTSCIQHLNTISKTIDNLTGRLLMPMMKAWLIFGTLCCTLLIMPFLHHYSSLNNHKIEQSIIKNSSFKGVKHDISNFSLIVTLAFGIGFLSCKFFFYYYHTYWTMXXXXXXXXXXXXXXXXXXXYATSEAATWPVCSVKLFQFNNAIVWIGLIGQFVFSVTMIMNSISHLSTEFSILGLCVLFEYLSKMIQISVQFILESLLLETQWE--NRKLEKNSKLINAFLFLMINNLCLTILNIARINDNILPPLKDPISFKIFFIFTTNFSMVFCVLQRLHGFLLFVRY 1507 G+ N++S+ +II N Y N NRN F K ++ N IL+ C P + I+ L I + ++ L G+ IF+ ++ I +++++ L + + D GE+W IN NM+ I IIS + ++ + ++Q+ + LN++ S +L + +++ L L+ K +IF C LI P ++H+ +L N K+ Q + + + + N SLI++ F + C Y + + ++ L +L ++ I + + P K+ + +W+GL GQF+ S+ I + + + +Y +++I+ Q I + ++E +W+ N+KL + ++ LFL+ +N L+ + + + ++F + M+F VL R+HG ++FV Y Sbjct: 67 GAFTNVMSSVLIIANMYHNFNNRNFFEKHKIPNQSILYLICLPSLVLILVGLSIVIMKEVLLKENSSVGKEIFSVSSLILFLSILSLLIEANMYKDSGEKWSINLLNMTTILFTIISLVNNLIYPLLIDQVQNYIVALNVS-MAKSETSNLYSALESLFELNSNLV---YKIIIIFNISCIFFLINPLVYHFKNLQNSKVSQLVQEEIPEDEEQPKLPNSSLILSFIFIVAICVC--IIYDWTSKQSLELINLLIVSTYIASFMHIYFILKNIQKCPRKD-KVNSIHYVFMWLGLFGQFIQSIAGSYAGIRLMLSNSDQNYWTRILQYFTQVIECFAQNICVTFIIERKWQPFNKKLWYS--FLSCPLFLVCSNTGFMFLSFYLLYSH--SAVSGQFHVQLFESGFNHLGMIFSVLHRIHGAIVFVYY 483
BLAST of SMED30015747 vs. Planmine SMEST
Match: SMESG000040959.1 (SMESG000040959.1) HSP 1 Score: 57.7658 bits (138), Expect = 1.245e-8 Identity = 110/454 (24.23%), Postives = 194/454 (42.73%), Query Frame = 2 Query: 230 GSVMNIVSASIIILNEYL--NHLNRNHFYKLRVLNSEILHTFCFPLVSFIIGSLLISTINDIYLIRCIGYGRMIFASTNSIYYITVMIFLAKYHFVCDDGEEWXXXXXXXXXXXXXXXXXXXXXXXXXSVEQITLIVPILNITDFDTSCIQHLNTISKTIDNLTGRLLMPMMKAW-----LIFGTLCCTLLIMPFLHHYSSLNNHKIEQSIIKNSSFKGVKHDISNF---------SLIVTLAFGIGFLSCKFFFYYYHTYWTMXXXXXXXXXXXXXXXXXXXYATSEAA---TWPVCS--VKLFQFNNAIVWIGLIGQFVFSVTMIMNSISHLSTEFSILGLCVLFEYLSKMIQISVQFILESLLLETQWENRKLEKNSKLINAFLFLMINNLCLTILNI-------ARINDNILPPLKDPISFKIFFIFTTNFSM--VFCVLQRLH-GFLLF 1498 G V+NIV +I++++Y H N F + + N I++ C P+ II ++I+ D Y + F + +Y+ ++++ L + + D GE W I FSN+ A+ I SQI+++ + S +Q +N +D C +SKT + L ++ + L+ G++C LL+ F Y +L +K E + ++ +S F S+ + +GF S K F Y I I + + ++ ++ WP S FN ++W G +GQ +T ++N +IL L + +Q SVQ I ES+L+E ++ + ++ I + L I N I+NI +ND L DP F+ +F+ F + +F ++Q+ H FLLF Sbjct: 51 GGVLNIVFIVLIVISKYFIRRHKNAKPFGETQAENPIIIYFICLPMTVSIIICMIITLFKDTYKPNSTR-DEIFFDTMAILYFGSLLLILIQLNGFYDFGENWEITFSNIFAVTSAIFSQIYFLSLFSSQQQAYFFTREVNNSDIKQEC----QNVSKTEIRIIFCALWTEVEKYVSKFHLVLGSICMLLLVKAFNKLYKNLQRYKKEDIFV----LPNMEEKLSRFGKTMIFICGSVNFSYCMALGFESNKTFRY------------ITSIAEIFVYFMMIYFSINQLGIIIKWPYRSDVENRDMFNEIMIWAGFLGQ-TMQLTALINYYDSSHKSLTIL------RNLIQFVQTSVQTIFESILIEKSQPSKNILFST--ITSLSVLAILNFQFGIINIWLFGQMLKDLNDK---SLNDP-KFQYYFMIILVFRVGTIFTIMQKFHSSFLLF 470 The following BLAST results are available for this feature:
BLAST of SMED30015747 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30015747 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30015747 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30015747 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30015747 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30015747 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30015747 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30015747 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30015747 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30015747 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30015747 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30015747 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30015747 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30015747 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 4
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30015747 ID=SMED30015747|Name=SMED30015747|organism=Schmidtea mediterranea sexual|type=transcript|length=1576bpback to top protein sequence of SMED30015747-orf-1 >SMED30015747-orf-1 ID=SMED30015747-orf-1|Name=SMED30015747-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=501bp MINYFNNIEIKTKTILEKLIFLIGLFLISFGSVTILGLIHQYFVKILSISback to top InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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