SMED30015727
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30015727 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Alignments
SMED30015727 aligns in the following genomic locations:
Homology
BLAST of SMED30015727 vs. Planmine SMEST
Match: SMESG000030475.1 (SMESG000030475.1) HSP 1 Score: 862.833 bits (2228), Expect = 0.000e+0 Identity = 436/441 (98.87%), Postives = 439/441 (99.55%), Query Frame = 1 Query: 55 MATQNLQLQATSLMDEIHNMSQSMNHSNMCKTIKKFTELLYSNRYLIYSNINEYFERIGLTGNLIKCLAQLPDQSKRDVLRLLHLFIKTNVNLRNQAGMSEDFMKYLSDTLEKVDTWNNTKNFKYXXXXXXXXXXDSNVPRYFKTNTIRQIVRQKIDELMFSENKLIYIVFYACSIRISSLEDVSISPDDAKAELIVSKEIIGIFIDVVRDYLDDFKTFYKDADFASNQYLLYRVKSEFSFDQICRTIIYFAVQSEKLRMNIFESGFYDLESNIFIIKYVPNEFLQNTLEIFKIPTIDQQIKQKFIAGYQRIFDRLSNMIRNGSVSTLIKNSVLNLFDLYGIPKSSSNVLITLPSNTNSKDSNFLSIDRWGSLDVDNWLSENNLEEYRNKFSGLTGRGLKQMKKLTMDEDGKMLIIDLLSTKLDISIIMIFEFLGALETLE 1377 MATQNLQLQATSLMDEIHNMSQSMNHSNMCKTIKKFTELLYSNRYLIYSNINEYFERIGLTGNLIKCLAQLPDQSKRDVLRLLHLFIKTN NL+NQAGMSEDFMKYLSDTLEKVDTWNNTKNFKYHIHIAAHIIIDSNVPRYFKTNTIRQIVRQKIDELMFSENKLIYIVFYACSIRISSLEDVSISPDDAKA+LIVSKEIIGIFIDVVRDYLDDFKTFYKDADFASNQYLLYRVKSEFSFDQICRTIIYFAVQSEKLRMNIFESGFYDL+SNIFIIKYVPNEFLQNTLEIFKIPTIDQQIKQKFIAGYQRIFDRLSNMIRNGSVSTLIKNSVLNLFDLYGIPK SSNVLITLPSNTNSKDSNFLSIDRWGSLDVDNWLSENNLEEYRNKFSGLTGRGLKQMKKLTMDEDGKMLIIDLLSTKLDISIIMIFEFLGALETLE Sbjct: 1 MATQNLQLQATSLMDEIHNMSQSMNHSNMCKTIKKFTELLYSNRYLIYSNINEYFERIGLTGNLIKCLAQLPDQSKRDVLRLLHLFIKTNANLQNQAGMSEDFMKYLSDTLEKVDTWNNTKNFKYHIHIAAHIIIDSNVPRYFKTNTIRQIVRQKIDELMFSENKLIYIVFYACSIRISSLEDVSISPDDAKADLIVSKEIIGIFIDVVRDYLDDFKTFYKDADFASNQYLLYRVKSEFSFDQICRTIIYFAVQSEKLRMNIFESGFYDLKSNIFIIKYVPNEFLQNTLEIFKIPTIDQQIKQKFIAGYQRIFDRLSNMIRNGSVSTLIKNSVLNLFDLYGIPKPSSNVLITLPSNTNSKDSNFLSIDRWGSLDVDNWLSENNLEEYRNKFSGLTGRGLKQMKKLTMDEDGKMLIIDLLSTKLDISIIMIFEFLGALETLE 441
BLAST of SMED30015727 vs. Planmine SMEST
Match: SMESG000030479.1 (SMESG000030479.1) HSP 1 Score: 288.886 bits (738), Expect = 8.973e-92 Identity = 170/463 (36.72%), Postives = 270/463 (58.32%), Query Frame = 1 Query: 55 MATQNLQLQATSLMDEIHNMSQSMNHSNMCKTIKKFTELLYSNRYLIYSNINEYFERIGLTGNLIKCLAQLPDQSKRDVLRLLHLFIKTNVNLRNQAGMSEDFMKYLSDTLEKVDTWNNTKNFKYXXXXXXXXXXDSNVPRYF--------KTNTIRQIVRQKIDELMFSEN---KLIYIVFYACSIRISSLEDVSISPDDAKAELIVSKEIIGIFIDVVRDYLDDFKTFYKDADFASNQYLLYRVKSEFSFDQICRTIIYFAVQSEKLRMNIFESGFYDLESNIFIIKYVPNEFLQNTLEIFKIPTIDQQIKQKFIAGYQRIFDRLSNMIRNGSVSTLIKNSVLNLFDLYGIPKS------------SSNVLITLPSNTNSKDSNFLSIDRWGSLDVDNWLSENNLEEYRNKFSGLTGRGLKQMKKLTMDEDGKMLIIDLLSTKLDISIIMIFEFLGALETL 1374 MA N ++ + ++ S ++ + +T T+ L++N LIYS +N+ FER GL L C+ L +Q +RD+LRLL+LF+KTN NLR+QA S+ M++L L + W KY D N+ Y T +IR+IVRQ ++EL +N +++Y++FY+ + + + + + A+ I+ KEI+G F++++RDY++DFK FY + D +++L Y K+E++FDQIC+ I+Y+A +K+R ++++SG Y+L S IF +K +P FL + L+IF+IP+ID ++ +F + I +RL N IR S S L++N L + Y +S + N LITLP NT K ++ I WGSLDVD WLSE+NL+ R F+ +T RG Q+ L + DGK +I+LL++KL+I +I + E LGALET+ Sbjct: 1 MAFSNCEMTGERYVSDLQEASSKKDYKALSETATTMTKYLFTNVDLIYSRVNDRFERSGLMLELFSCMDNLNEQFRRDILRLLNLFLKTNKNLRSQASKSQSIMQFLLKRLSSREKWETVDKIKY----------DVNLSSYLLLTRASQENTESIRRIVRQILEELKNDKNLQKRILYMIFYSSAEINAGFQKKELGLE-AENNGIMVKEILGDFVEMIRDYINDFKIFYDNTDNEQSKFLTYNYKAEYTFDQICQCIVYYAKTCKKVREDLYQSGLYELASTIFTMKSIPEVFLLSLLDIFEIPSIDNTLRDRFSKEHNFIIERLEN-IRTNS-SNLVRNGAERLIEKYRDCQSLKSKDISKQFPQTDNKLITLP-NTEQKFAHN-GISDWGSLDVDQWLSEHNLQACRKPFTSMTSRGFTQLMFLMKENDGKFKLIELLTSKLNIDLISVLELLGALETI 448
BLAST of SMED30015727 vs. Planmine SMEST
Match: SMESG000030479.1 (SMESG000030479.1) HSP 1 Score: 283.108 bits (723), Expect = 1.070e-89 Identity = 167/450 (37.11%), Postives = 265/450 (58.89%), Query Frame = 1 Query: 94 MDEIHNMSQSMNHSNMCKTIKKFTELLYSNRYLIYSNINEYFERIGLTGNLIKCLAQLPDQSKRDVLRLLHLFIKTNVNLRNQAGMSEDFMKYLSDTLEKVDTWNNTKNFKYXXXXXXXXXXDSNVPRYF--------KTNTIRQIVRQKIDELMFSEN---KLIYIVFYACSIRISSLEDVSISPDDAKAELIVSKEIIGIFIDVVRDYLDDFKTFYKDADFASNQYLLYRVKSEFSFDQICRTIIYFAVQSEKLRMNIFESGFYDLESNIFIIKYVPNEFLQNTLEIFKIPTIDQQIKQKFIAGYQRIFDRLSNMIRNGSVSTLIKNSVLNLFDLYGIPKS------------SSNVLITLPSNTNSKDSNFLSIDRWGSLDVDNWLSENNLEEYRNKFSGLTGRGLKQMKKLTMDEDGKMLIIDLLSTKLDISIIMIFEFLGALETL 1374 + ++ S ++ + +T T+ L++N LIYS +N+ FER GL L C+ L +Q +RD+LRLL+LF+KTN NLR+QA S+ M++L L + W KY D N+ Y T +IR+IVRQ ++EL +N +++Y++FY+ + + + + + A+ I+ KEI+G F++++RDY++DFK FY + D +++L Y K+E++FDQIC+ I+Y+A +K+R ++++SG Y+L S IF +K +P FL + L+IF+IP+ID ++ +F + I +RL N IR S S L++N L + Y +S + N LITLP NT K ++ I WGSLDVD WLSE+NL+ R F+ +T RG Q+ L + DGK +I+LL++KL+I +I + E LGALET+ Sbjct: 7 VSDLQEASSKKDYKALSETATTMTKYLFTNVDLIYSRVNDRFERSGLMLELFSCMDNLNEQFRRDILRLLNLFLKTNKNLRSQASKSQSIMQFLLKRLSSREKWETVDKIKY----------DVNLSSYLLLTRASQENTESIRRIVRQILEELKNDKNLQKRILYMIFYSSAEINAGFQKKELGLE-AENNGIMVKEILGDFVEMIRDYINDFKIFYDNTDNEQSKFLTYNYKAEYTFDQICQCIVYYAKTCKKVREDLYQSGLYELASTIFTMKSIPEVFLLSLLDIFEIPSIDNTLRDRFSKEHNFIIERLEN-IRTNS-SNLVRNGAERLIEKYRDCQSLKSKDISKQFPQTDNKLITLP-NTEQKFAHN-GISDWGSLDVDQWLSEHNLQACRKPFTSMTSRGFTQLMFLMKENDGKFKLIELLTSKLNIDLISVLELLGALETI 441
BLAST of SMED30015727 vs. Planmine SMEST
Match: SMESG000030473.1 (SMESG000030473.1) HSP 1 Score: 251.136 bits (640), Expect = 2.718e-77 Identity = 167/457 (36.54%), Postives = 253/457 (55.36%), Query Frame = 1 Query: 55 MATQNLQLQATSLMDEIHNMSQSMNHSNMCKTIKKFTELLYSNRYLIYSNINEYFERIGLTGNLIKCLAQLPDQSKRDVLRLLHLFIKTNVNLRNQAGMSEDFMKYLSDTLEKVDTWNNTKNFKYXXXXXXXXXXDSNVPRYFKTNTIRQIVRQKIDELMFSENKLIYIVFYACSIRI-SSLEDVSISPDDAKAELIVSKEIIGIFIDVVRDYLDDFKTFYKDADFASNQYLLYRVKSEFSFDQICRTIIYFAVQSEKLRMNIFESGFYDLESNIFIIKYVPNEFLQNTLEIFKIPTIDQQIKQKFIAGYQRIFDRLSNMIRNGSVSTLIKNS--VL----------NLFDLYGIPKSSSNVLITLPSNTNSKDSNFLSIDRWG----SLDVDNWLSENNLEEYRNKFSGLTGRGLKQMKKLTMDEDGKMLIIDLLSTKLDISIIMIFEFLGALETL 1374 MA + Q +A + +DEIH++S+S + M I LYSN+ ++YSNIN YF +IGL L + ++ +DVLRLLH+FIKTN N++ G SE F+KYLS L WNN F+YHIHIAA++++D ++F TIR I+R KI E + SEN ++ V YAC++ I SS + S E + S +G F ++ Y ++FKTFYK+++ +N+Y L+ + +E++FDQIC +I Y++ SE++ NIF S Y+ S I I+ +P F+ +L I IP+I ++ +I L++ N S+ + ++S VL +L+D + L+++ S N K SI SL VD WL +N L R F L+ +GL QMK L D ++ +I+LL+TKLD+ II EFL L+ L Sbjct: 1 MALNSAQTKAIAYIDEIHSLSRSNRFTLMLSKINNLYIFLYSNKSILYSNINRYFYKIGLVPILTNYCHYISHETMKDVLRLLHIFIKTNKNIQEAVGQSEKFVKYLSAILCTESAWNNIGTFRYHIHIAAYLLLDQTCWQHFDIATIRAIIRGKIHEDIHSENNFLHTVCYACTMPITSSAASGNTSLTSVTDEDLFSLSAMGGFSPLINSYFEEFKTFYKNSENTTNEYSLFLLDTEYAFDQICHSISYYSRLSERICANIFNSDIYESISGILAIRNIPEHFISRSLHILSIPSIYSHPDERIRKETNQISYALTSYFDNDSLPSDNQDSGKVLPNKYWSYESGSLYDTDDFSMKGKHTLLSIESENNLKSK---SIGEKSTVKMSLTVDEWLIKNRLYYLRAPFDQLSEKGLYQMKTLITTPDNRIRLIELLTTKLDLDIIGTLEFLECLDRL 454
BLAST of SMED30015727 vs. Planmine SMEST
Match: SMESG000030473.1 (SMESG000030473.1) HSP 1 Score: 207.608 bits (527), Expect = 1.837e-61 Identity = 138/378 (36.51%), Postives = 210/378 (55.56%), Query Frame = 1 Query: 283 RDVLRLLHLFIKTNVNLRNQAGMSEDFMKYLSDTLEKVDTWNNTKNFKYXXXXXXXXXXDSNVPRYFKTNTIRQIVRQKIDELMFSENKLIYIVFYACSIRI-SSLEDVSISPDDAKAELIVSKEIIGIFIDVVRDYLDDFKTFYKDADFASNQYLLYRVKSEFSFDQICRTIIYFAVQSEKLRMNIFESGFYDLESNIFIIKYVPNEFLQNTLEIFKIPTIDQQIKQKFIAGYQRIFDRLSNMIRNGSVSTLIKNS--VL----------NLFDLYGIPKSSSNVLITLPSNTNSKDSNFLSIDRWG-SLDVDNWLSENNLEEYRNKFSGLTGRGLKQMKKLTMDEDGKMLIIDLLSTKLDISIIMIFEFLGALETL 1374 +DVLRLLH+FIKTN N++ G SE F+KYLS L WNN F+YHIHIAA++++D ++F TIR I+R KI E + SEN ++ V YAC++ I SS + S E + S +G F ++ Y ++FKTFYK+++ +N+Y L+ + +E++FDQIC +I Y++ SE++ NIF S Y+ S I I+ +P F+ +L I IP+I ++ +I L++ N S+ + ++S VL +L+D + L+++ S N K + SL VD WL +N L R F L+ +GL QMK L D ++ +I+LL+TKLD+ II EFL L+ L Sbjct: 2 KDVLRLLHIFIKTNKNIQEAVGQSEKFVKYLSAILCTESAWNNIGTFRYHIHIAAYLLLDQTCWQHFDIATIRAIIRGKIHEDIHSENNFLHTVCYACTMPITSSAASGNTSLTSVTDEDLFSLSAMGGFSPLINSYFEEFKTFYKNSENTTNEYSLFLLDTEYAFDQICHSISYYSRLSERICANIFNSDIYESISGILAIRNIPEHFISRSLHILSIPSIYSHPDERIRKETNQISYALTSYFDNDSLPSDNQDSGKVLPNKYWSYESGSLYDTDDFSMKGKHTLLSIESENNLKSKSIGEKSTVKMSLTVDEWLIKNRLYYLRAPFDQLSEKGLYQMKTLITTPDNRIRLIELLTTKLDLDIIGTLEFLECLDRL 379 The following BLAST results are available for this feature:
BLAST of SMED30015727 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30015727 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30015727 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30015727 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30015727 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30015727 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30015727 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30015727 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30015727 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30015727 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30015727 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30015727 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30015727 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30015727 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30015727 ID=SMED30015727|Name=SMED30015727|organism=Schmidtea mediterranea sexual|type=transcript|length=1629bpback to top protein sequence of SMED30015727-orf-1 >SMED30015727-orf-1 ID=SMED30015727-orf-1|Name=SMED30015727-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=442bp MATQNLQLQATSLMDEIHNMSQSMNHSNMCKTIKKFTELLYSNRYLIYSNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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