SMED30015727

Overview
NameSMED30015727
Smed IDSMED30015727
Length (bp)1629
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30015727 (SMED30015727) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30015727 (SMED30015727) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30015727 (SMED30015727) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30015727 (SMED30015727) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30015727

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
cephalic gangliaSMED30015727SMESG000030475.1 dd_Smed_v4_3112_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30015727SMESG000030475.1 dd_Smed_v4_3112_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30015727SMESG000030475.1 dd_Smed_v4_3112_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30015727 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.019611:558..2197 +7844
Homology
[Close]
BLAST of SMED30015727 vs. Planmine SMEST
Match: SMESG000030475.1 (SMESG000030475.1)

HSP 1 Score: 862.833 bits (2228), Expect = 0.000e+0
Identity = 436/441 (98.87%), Postives = 439/441 (99.55%), Query Frame = 1 
Query:   55 MATQNLQLQATSLMDEIHNMSQSMNHSNMCKTIKKFTELLYSNRYLIYSNINEYFERIGLTGNLIKCLAQLPDQSKRDVLRLLHLFIKTNVNLRNQAGMSEDFMKYLSDTLEKVDTWNNTKNFKYXXXXXXXXXXDSNVPRYFKTNTIRQIVRQKIDELMFSENKLIYIVFYACSIRISSLEDVSISPDDAKAELIVSKEIIGIFIDVVRDYLDDFKTFYKDADFASNQYLLYRVKSEFSFDQICRTIIYFAVQSEKLRMNIFESGFYDLESNIFIIKYVPNEFLQNTLEIFKIPTIDQQIKQKFIAGYQRIFDRLSNMIRNGSVSTLIKNSVLNLFDLYGIPKSSSNVLITLPSNTNSKDSNFLSIDRWGSLDVDNWLSENNLEEYRNKFSGLTGRGLKQMKKLTMDEDGKMLIIDLLSTKLDISIIMIFEFLGALETLE 1377
            MATQNLQLQATSLMDEIHNMSQSMNHSNMCKTIKKFTELLYSNRYLIYSNINEYFERIGLTGNLIKCLAQLPDQSKRDVLRLLHLFIKTN NL+NQAGMSEDFMKYLSDTLEKVDTWNNTKNFKYHIHIAAHIIIDSNVPRYFKTNTIRQIVRQKIDELMFSENKLIYIVFYACSIRISSLEDVSISPDDAKA+LIVSKEIIGIFIDVVRDYLDDFKTFYKDADFASNQYLLYRVKSEFSFDQICRTIIYFAVQSEKLRMNIFESGFYDL+SNIFIIKYVPNEFLQNTLEIFKIPTIDQQIKQKFIAGYQRIFDRLSNMIRNGSVSTLIKNSVLNLFDLYGIPK SSNVLITLPSNTNSKDSNFLSIDRWGSLDVDNWLSENNLEEYRNKFSGLTGRGLKQMKKLTMDEDGKMLIIDLLSTKLDISIIMIFEFLGALETLE
Sbjct:    1 MATQNLQLQATSLMDEIHNMSQSMNHSNMCKTIKKFTELLYSNRYLIYSNINEYFERIGLTGNLIKCLAQLPDQSKRDVLRLLHLFIKTNANLQNQAGMSEDFMKYLSDTLEKVDTWNNTKNFKYHIHIAAHIIIDSNVPRYFKTNTIRQIVRQKIDELMFSENKLIYIVFYACSIRISSLEDVSISPDDAKADLIVSKEIIGIFIDVVRDYLDDFKTFYKDADFASNQYLLYRVKSEFSFDQICRTIIYFAVQSEKLRMNIFESGFYDLKSNIFIIKYVPNEFLQNTLEIFKIPTIDQQIKQKFIAGYQRIFDRLSNMIRNGSVSTLIKNSVLNLFDLYGIPKPSSNVLITLPSNTNSKDSNFLSIDRWGSLDVDNWLSENNLEEYRNKFSGLTGRGLKQMKKLTMDEDGKMLIIDLLSTKLDISIIMIFEFLGALETLE 441
[Close]
BLAST of SMED30015727 vs. Planmine SMEST
Match: SMESG000030479.1 (SMESG000030479.1)

HSP 1 Score: 288.886 bits (738), Expect = 8.973e-92
Identity = 170/463 (36.72%), Postives = 270/463 (58.32%), Query Frame = 1 
Query:   55 MATQNLQLQATSLMDEIHNMSQSMNHSNMCKTIKKFTELLYSNRYLIYSNINEYFERIGLTGNLIKCLAQLPDQSKRDVLRLLHLFIKTNVNLRNQAGMSEDFMKYLSDTLEKVDTWNNTKNFKYXXXXXXXXXXDSNVPRYF--------KTNTIRQIVRQKIDELMFSEN---KLIYIVFYACSIRISSLEDVSISPDDAKAELIVSKEIIGIFIDVVRDYLDDFKTFYKDADFASNQYLLYRVKSEFSFDQICRTIIYFAVQSEKLRMNIFESGFYDLESNIFIIKYVPNEFLQNTLEIFKIPTIDQQIKQKFIAGYQRIFDRLSNMIRNGSVSTLIKNSVLNLFDLYGIPKS------------SSNVLITLPSNTNSKDSNFLSIDRWGSLDVDNWLSENNLEEYRNKFSGLTGRGLKQMKKLTMDEDGKMLIIDLLSTKLDISIIMIFEFLGALETL 1374
            MA  N ++     + ++   S   ++  + +T    T+ L++N  LIYS +N+ FER GL   L  C+  L +Q +RD+LRLL+LF+KTN NLR+QA  S+  M++L   L   + W      KY          D N+  Y          T +IR+IVRQ ++EL   +N   +++Y++FY+ +   +  +   +  + A+   I+ KEI+G F++++RDY++DFK FY + D   +++L Y  K+E++FDQIC+ I+Y+A   +K+R ++++SG Y+L S IF +K +P  FL + L+IF+IP+ID  ++ +F   +  I +RL N IR  S S L++N    L + Y   +S            + N LITLP NT  K ++   I  WGSLDVD WLSE+NL+  R  F+ +T RG  Q+  L  + DGK  +I+LL++KL+I +I + E LGALET+
Sbjct:    1 MAFSNCEMTGERYVSDLQEASSKKDYKALSETATTMTKYLFTNVDLIYSRVNDRFERSGLMLELFSCMDNLNEQFRRDILRLLNLFLKTNKNLRSQASKSQSIMQFLLKRLSSREKWETVDKIKY----------DVNLSSYLLLTRASQENTESIRRIVRQILEELKNDKNLQKRILYMIFYSSAEINAGFQKKELGLE-AENNGIMVKEILGDFVEMIRDYINDFKIFYDNTDNEQSKFLTYNYKAEYTFDQICQCIVYYAKTCKKVREDLYQSGLYELASTIFTMKSIPEVFLLSLLDIFEIPSIDNTLRDRFSKEHNFIIERLEN-IRTNS-SNLVRNGAERLIEKYRDCQSLKSKDISKQFPQTDNKLITLP-NTEQKFAHN-GISDWGSLDVDQWLSEHNLQACRKPFTSMTSRGFTQLMFLMKENDGKFKLIELLTSKLNIDLISVLELLGALETI 448
[Close]
BLAST of SMED30015727 vs. Planmine SMEST
Match: SMESG000030479.1 (SMESG000030479.1)

HSP 1 Score: 283.108 bits (723), Expect = 1.070e-89
Identity = 167/450 (37.11%), Postives = 265/450 (58.89%), Query Frame = 1 
Query:   94 MDEIHNMSQSMNHSNMCKTIKKFTELLYSNRYLIYSNINEYFERIGLTGNLIKCLAQLPDQSKRDVLRLLHLFIKTNVNLRNQAGMSEDFMKYLSDTLEKVDTWNNTKNFKYXXXXXXXXXXDSNVPRYF--------KTNTIRQIVRQKIDELMFSEN---KLIYIVFYACSIRISSLEDVSISPDDAKAELIVSKEIIGIFIDVVRDYLDDFKTFYKDADFASNQYLLYRVKSEFSFDQICRTIIYFAVQSEKLRMNIFESGFYDLESNIFIIKYVPNEFLQNTLEIFKIPTIDQQIKQKFIAGYQRIFDRLSNMIRNGSVSTLIKNSVLNLFDLYGIPKS------------SSNVLITLPSNTNSKDSNFLSIDRWGSLDVDNWLSENNLEEYRNKFSGLTGRGLKQMKKLTMDEDGKMLIIDLLSTKLDISIIMIFEFLGALETL 1374
            + ++   S   ++  + +T    T+ L++N  LIYS +N+ FER GL   L  C+  L +Q +RD+LRLL+LF+KTN NLR+QA  S+  M++L   L   + W      KY          D N+  Y          T +IR+IVRQ ++EL   +N   +++Y++FY+ +   +  +   +  + A+   I+ KEI+G F++++RDY++DFK FY + D   +++L Y  K+E++FDQIC+ I+Y+A   +K+R ++++SG Y+L S IF +K +P  FL + L+IF+IP+ID  ++ +F   +  I +RL N IR  S S L++N    L + Y   +S            + N LITLP NT  K ++   I  WGSLDVD WLSE+NL+  R  F+ +T RG  Q+  L  + DGK  +I+LL++KL+I +I + E LGALET+
Sbjct:    7 VSDLQEASSKKDYKALSETATTMTKYLFTNVDLIYSRVNDRFERSGLMLELFSCMDNLNEQFRRDILRLLNLFLKTNKNLRSQASKSQSIMQFLLKRLSSREKWETVDKIKY----------DVNLSSYLLLTRASQENTESIRRIVRQILEELKNDKNLQKRILYMIFYSSAEINAGFQKKELGLE-AENNGIMVKEILGDFVEMIRDYINDFKIFYDNTDNEQSKFLTYNYKAEYTFDQICQCIVYYAKTCKKVREDLYQSGLYELASTIFTMKSIPEVFLLSLLDIFEIPSIDNTLRDRFSKEHNFIIERLEN-IRTNS-SNLVRNGAERLIEKYRDCQSLKSKDISKQFPQTDNKLITLP-NTEQKFAHN-GISDWGSLDVDQWLSEHNLQACRKPFTSMTSRGFTQLMFLMKENDGKFKLIELLTSKLNIDLISVLELLGALETI 441
[Close]
BLAST of SMED30015727 vs. Planmine SMEST
Match: SMESG000030473.1 (SMESG000030473.1)

HSP 1 Score: 251.136 bits (640), Expect = 2.718e-77
Identity = 167/457 (36.54%), Postives = 253/457 (55.36%), Query Frame = 1 
Query:   55 MATQNLQLQATSLMDEIHNMSQSMNHSNMCKTIKKFTELLYSNRYLIYSNINEYFERIGLTGNLIKCLAQLPDQSKRDVLRLLHLFIKTNVNLRNQAGMSEDFMKYLSDTLEKVDTWNNTKNFKYXXXXXXXXXXDSNVPRYFKTNTIRQIVRQKIDELMFSENKLIYIVFYACSIRI-SSLEDVSISPDDAKAELIVSKEIIGIFIDVVRDYLDDFKTFYKDADFASNQYLLYRVKSEFSFDQICRTIIYFAVQSEKLRMNIFESGFYDLESNIFIIKYVPNEFLQNTLEIFKIPTIDQQIKQKFIAGYQRIFDRLSNMIRNGSVSTLIKNS--VL----------NLFDLYGIPKSSSNVLITLPSNTNSKDSNFLSIDRWG----SLDVDNWLSENNLEEYRNKFSGLTGRGLKQMKKLTMDEDGKMLIIDLLSTKLDISIIMIFEFLGALETL 1374
            MA  + Q +A + +DEIH++S+S   + M   I      LYSN+ ++YSNIN YF +IGL   L      +  ++ +DVLRLLH+FIKTN N++   G SE F+KYLS  L     WNN   F+YHIHIAA++++D    ++F   TIR I+R KI E + SEN  ++ V YAC++ I SS    + S      E + S   +G F  ++  Y ++FKTFYK+++  +N+Y L+ + +E++FDQIC +I Y++  SE++  NIF S  Y+  S I  I+ +P  F+  +L I  IP+I     ++      +I   L++   N S+ +  ++S  VL          +L+D         + L+++ S  N K     SI        SL VD WL +N L   R  F  L+ +GL QMK L    D ++ +I+LL+TKLD+ II   EFL  L+ L
Sbjct:    1 MALNSAQTKAIAYIDEIHSLSRSNRFTLMLSKINNLYIFLYSNKSILYSNINRYFYKIGLVPILTNYCHYISHETMKDVLRLLHIFIKTNKNIQEAVGQSEKFVKYLSAILCTESAWNNIGTFRYHIHIAAYLLLDQTCWQHFDIATIRAIIRGKIHEDIHSENNFLHTVCYACTMPITSSAASGNTSLTSVTDEDLFSLSAMGGFSPLINSYFEEFKTFYKNSENTTNEYSLFLLDTEYAFDQICHSISYYSRLSERICANIFNSDIYESISGILAIRNIPEHFISRSLHILSIPSIYSHPDERIRKETNQISYALTSYFDNDSLPSDNQDSGKVLPNKYWSYESGSLYDTDDFSMKGKHTLLSIESENNLKSK---SIGEKSTVKMSLTVDEWLIKNRLYYLRAPFDQLSEKGLYQMKTLITTPDNRIRLIELLTTKLDLDIIGTLEFLECLDRL 454
[Close]
BLAST of SMED30015727 vs. Planmine SMEST
Match: SMESG000030473.1 (SMESG000030473.1)

HSP 1 Score: 207.608 bits (527), Expect = 1.837e-61
Identity = 138/378 (36.51%), Postives = 210/378 (55.56%), Query Frame = 1 
Query:  283 RDVLRLLHLFIKTNVNLRNQAGMSEDFMKYLSDTLEKVDTWNNTKNFKYXXXXXXXXXXDSNVPRYFKTNTIRQIVRQKIDELMFSENKLIYIVFYACSIRI-SSLEDVSISPDDAKAELIVSKEIIGIFIDVVRDYLDDFKTFYKDADFASNQYLLYRVKSEFSFDQICRTIIYFAVQSEKLRMNIFESGFYDLESNIFIIKYVPNEFLQNTLEIFKIPTIDQQIKQKFIAGYQRIFDRLSNMIRNGSVSTLIKNS--VL----------NLFDLYGIPKSSSNVLITLPSNTNSKDSNFLSIDRWG-SLDVDNWLSENNLEEYRNKFSGLTGRGLKQMKKLTMDEDGKMLIIDLLSTKLDISIIMIFEFLGALETL 1374
            +DVLRLLH+FIKTN N++   G SE F+KYLS  L     WNN   F+YHIHIAA++++D    ++F   TIR I+R KI E + SEN  ++ V YAC++ I SS    + S      E + S   +G F  ++  Y ++FKTFYK+++  +N+Y L+ + +E++FDQIC +I Y++  SE++  NIF S  Y+  S I  I+ +P  F+  +L I  IP+I     ++      +I   L++   N S+ +  ++S  VL          +L+D         + L+++ S  N K  +         SL VD WL +N L   R  F  L+ +GL QMK L    D ++ +I+LL+TKLD+ II   EFL  L+ L
Sbjct:    2 KDVLRLLHIFIKTNKNIQEAVGQSEKFVKYLSAILCTESAWNNIGTFRYHIHIAAYLLLDQTCWQHFDIATIRAIIRGKIHEDIHSENNFLHTVCYACTMPITSSAASGNTSLTSVTDEDLFSLSAMGGFSPLINSYFEEFKTFYKNSENTTNEYSLFLLDTEYAFDQICHSISYYSRLSERICANIFNSDIYESISGILAIRNIPEHFISRSLHILSIPSIYSHPDERIRKETNQISYALTSYFDNDSLPSDNQDSGKVLPNKYWSYESGSLYDTDDFSMKGKHTLLSIESENNLKSKSIGEKSTVKMSLTVDEWLIKNRLYYLRAPFDQLSEKGLYQMKTLITTPDNRIRLIELLTTKLDLDIIGTLEFLECLDRL 379
The following BLAST results are available for this feature:
BLAST of SMED30015727 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015727 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015727 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015727 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015727 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015727 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015727 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015727 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015727 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015727 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015727 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015727 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015727 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015727 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000030475.10.000e+098.87SMESG000030475.1[more]
SMESG000030479.18.973e-9236.72SMESG000030479.1[more]
SMESG000030479.11.070e-8937.11SMESG000030479.1[more]
SMESG000030473.12.718e-7736.54SMESG000030473.1[more]
SMESG000030473.11.837e-6136.51SMESG000030473.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015727 ID=SMED30015727|Name=SMED30015727|organism=Schmidtea mediterranea sexual|type=transcript|length=1629bp
TTATGATTCATTTGTTATTATTGCAGAATTGAAAATTGATTTACATTGAT
TGGAATGGCAACTCAGAATCTCCAGCTCCAAGCTACATCTTTAATGGACG
AAATACACAATATGTCTCAGTCCATGAACCATTCGAATATGTGTAAAACC
ATCAAAAAGTTTACCGAACTTCTGTATTCAAACCGATACCTTATTTATTC
CAATATCAACGAATATTTCGAAAGAATTGGACTGACAGGTAACCTCATAA
AATGTCTAGCCCAATTACCTGACCAGTCAAAGAGAGATGTTTTACGACTG
TTGCATTTGTTTATTAAAACCAATGTAAACCTTCGAAATCAAGCCGGAAT
GTCAGAAGATTTCATGAAATATCTTTCAGATACTTTAGAAAAGGTTGATA
CCTGGAACAATACTAAAAACTTTAAATACCACATCCATATTGCGGCACAC
ATAATTATAGATTCCAATGTGCCGAGGTATTTTAAAACAAATACCATTCG
ACAAATTGTTCGTCAAAAAATTGACGAACTAATGTTCAGTGAGAACAAAT
TAATTTATATTGTCTTTTATGCATGCTCGATACGGATATCAAGTTTAGAA
GACGTTTCAATATCTCCAGATGACGCAAAGGCTGAATTAATCGTATCTAA
AGAAATAATTGGAATCTTCATCGATGTCGTTCGGGATTATCTTGACGATT
TCAAAACATTCTACAAAGATGCTGATTTTGCAAGCAATCAGTATTTGTTG
TATCGAGTCAAATCAGAATTCTCATTTGACCAAATCTGCAGAACGATCAT
ATATTTTGCGGTCCAAAGCGAAAAGCTGAGGATGAATATCTTTGAGAGTG
GATTCTATGATTTAGAGTCCAATATCTTCATCATAAAGTACGTTCCAAAT
GAATTTCTTCAGAACACTTTAGAGATCTTCAAGATCCCTACTATAGACCA
GCAAATCAAGCAGAAATTCATCGCTGGTTATCAAAGAATTTTCGATAGAC
TTTCAAATATGATCAGGAATGGATCGGTGTCAACCTTGATTAAAAATTCT
GTATTAAACTTGTTTGATCTTTATGGCATTCCGAAATCATCTTCTAATGT
TCTCATTACCTTACCTAGTAATACTAACTCCAAGGATTCTAATTTTTTAA
GTATTGACAGATGGGGTTCCTTGGACGTTGACAATTGGCTCTCGGAGAAT
AACTTAGAGGAATATCGAAATAAATTTTCGGGTCTGACCGGTCGAGGACT
GAAGCAGATGAAAAAGTTAACAATGGATGAGGACGGCAAAATGCTGATCA
TTGACCTGTTGTCAACGAAATTGGATATTAGTATCATAATGATATTTGAA
TTTCTAGGAGCACTGGAAACTCTTGAATGACACGCAGAATTCAGATTGGT
TTAACTTGCGAAAGAACTAAGTTTTGATTATATAAATGTAATAATAATAT
TAATATAAAACTTTCTGTGGTGAATTTTGCATTTGTTATAACGTTTATAA
ATGATTTTATGTCATTTCATCACGCGGCATTCTATCAAGTTGAATATTAA
TTGTAAAATAAAAAAATTGTGAGTTGCGTTAATCTTATAGACCATTCTGC
TAGCATTAGTTATTATTGTGGACTTGCAT
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protein sequence of SMED30015727-orf-1

>SMED30015727-orf-1 ID=SMED30015727-orf-1|Name=SMED30015727-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=442bp
MATQNLQLQATSLMDEIHNMSQSMNHSNMCKTIKKFTELLYSNRYLIYSN
INEYFERIGLTGNLIKCLAQLPDQSKRDVLRLLHLFIKTNVNLRNQAGMS
EDFMKYLSDTLEKVDTWNNTKNFKYHIHIAAHIIIDSNVPRYFKTNTIRQ
IVRQKIDELMFSENKLIYIVFYACSIRISSLEDVSISPDDAKAELIVSKE
IIGIFIDVVRDYLDDFKTFYKDADFASNQYLLYRVKSEFSFDQICRTIIY
FAVQSEKLRMNIFESGFYDLESNIFIIKYVPNEFLQNTLEIFKIPTIDQQ
IKQKFIAGYQRIFDRLSNMIRNGSVSTLIKNSVLNLFDLYGIPKSSSNVL
ITLPSNTNSKDSNFLSIDRWGSLDVDNWLSENNLEEYRNKFSGLTGRGLK
QMKKLTMDEDGKMLIIDLLSTKLDISIIMIFEFLGALETLE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR013761SAM/pointed_sf
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013761Sterile alpha motif/pointed domain superfamilyGENE3DG3DSA:1.10.150.50coord: 363..423
e-value: 5.6E-6
score: 28.0