Heat shock 70 kDa protein

Overview


Name	Heat shock 70 kDa protein
Smed ID	SMED30015695
Length (bp)	482

Neoblast Clusters

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30015695

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 2

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication	Specimen	Lifecycle	Evidence
intestinal phagocyte	SMED30015695	SMESG000080786.1	Contig3521	newmark_ests	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30015695	SMESG000080786.1	Contig3521	uc_Smed_v2	PMID:23079596 Forsthoefel et al., 2012

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Human
Match: HSPA2 (heat shock protein family A (Hsp70) member 2 [Source:HGNC Symbol;Acc:HGNC:5235])

HSP 1 Score: 68.1662 bits (165), Expect = 3.274e-18
Identity = 33/46 (71.74%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G AIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    5 GPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 50

HSP 2 Score: 41.5874 bits (96), Expect = 3.274e-18
Identity = 20/32 (62.50%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +TIF AKRLIG
Sbjct:   45 FTDTERLIGDAAKNQVAMNPTNTIFDAKRLIG 76

[Close]

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G AIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    5 GPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 50

HSP 2 Score: 41.5874 bits (96), Expect = 3.274e-18
Identity = 20/32 (62.50%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +TIF AKRLIG
Sbjct:   45 FTDTERLIGDAAKNQVAMNPTNTIFDAKRLIG 76

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Human
Match: HSPA8 (heat shock protein family A (Hsp70) member 8 [Source:HGNC Symbol;Acc:HGNC:5241])

HSP 1 Score: 67.781 bits (164), Expect = 4.607e-18
Identity = 32/46 (69.57%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G A+GI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    4 GPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 49

HSP 2 Score: 41.2022 bits (95), Expect = 4.607e-18
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +T+F AKRLIG
Sbjct:   44 FTDTERLIGDAAKNQVAMNPTNTVFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Human
Match: HSPA8 (heat shock protein family A (Hsp70) member 8 [Source:HGNC Symbol;Acc:HGNC:5241])

HSP 1 Score: 67.781 bits (164), Expect = 4.895e-18
Identity = 32/46 (69.57%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G A+GI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    4 GPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 49

HSP 2 Score: 41.2022 bits (95), Expect = 4.895e-18
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +T+F AKRLIG
Sbjct:   44 FTDTERLIGDAAKNQVAMNPTNTVFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Human
Match: HSPA8 (heat shock protein family A (Hsp70) member 8 [Source:HGNC Symbol;Acc:HGNC:5241])

HSP 1 Score: 67.781 bits (164), Expect = 5.053e-18
Identity = 32/46 (69.57%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G A+GI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    4 GPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 49

HSP 2 Score: 41.2022 bits (95), Expect = 5.053e-18
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +T+F AKRLIG
Sbjct:   44 FTDTERLIGDAAKNQVAMNPTNTVFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Celegans
Match: hsp-1 (Heat shock 70 kDa protein A [Source:UniProtKB/Swiss-Prot;Acc:P09446])

HSP 1 Score: 66.6254 bits (161), Expect = 3.032e-18
Identity = 31/45 (68.89%), Postives = 35/45 (77.78%), Query Frame = 3

Query:  255 NAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            NA+GI L T YSCVGV    K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    5 NAVGIDLGTTYSCVGVFMHGKVEIIANDQGNRTTPSYVAFTDTER 49

HSP 2 Score: 41.5874 bits (96), Expect = 3.032e-18
Identity = 19/32 (59.38%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  ++T+F AKRLIG
Sbjct:   44 FTDTERLIGDAAKNQVAMNPHNTVFDAKRLIG 75

[Close]

Query:  255 NAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            NA+GI L T YSCVGV    K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    5 NAVGIDLGTTYSCVGVFMHGKVEIIANDQGNRTTPSYVAFTDTER 49

HSP 2 Score: 41.5874 bits (96), Expect = 3.032e-18
Identity = 19/32 (59.38%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  ++T+F AKRLIG
Sbjct:   44 FTDTERLIGDAAKNQVAMNPHNTVFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Celegans
Match: hsp-3 (Heat shock 70 kDa protein C [Source:UniProtKB/Swiss-Prot;Acc:P27420])

HSP 1 Score: 57.7658 bits (138), Expect = 7.034e-14
Identity = 30/49 (61.22%), Postives = 34/49 (69.39%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKKD*L 398
            G  IGI L T YSCVGV +  + EIIANDQ NR TPSYVAF+  + D L
Sbjct:   33 GTIIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFSGDQGDRL 81

HSP 2 Score: 35.8094 bits (81), Expect = 7.034e-14
Identity = 18/27 (66.67%), Postives = 21/27 (77.78%), Query Frame = 2

Query:  389 RLITDAAKNQVRMKCNDTIFYAKRLIG 469
            RLI DAAKNQ+ +   +TIF AKRLIG
Sbjct:   80 RLIGDAAKNQLTINPENTIFDAKRLIG 106

[Close]

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKKD*L 398
            G  IGI L T YSCVGV +  + EIIANDQ NR TPSYVAF+  + D L
Sbjct:   33 GTIIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFSGDQGDRL 81

HSP 2 Score: 35.8094 bits (81), Expect = 7.034e-14
Identity = 18/27 (66.67%), Postives = 21/27 (77.78%), Query Frame = 2

Query:  389 RLITDAAKNQVRMKCNDTIFYAKRLIG 469
            RLI DAAKNQ+ +   +TIF AKRLIG
Sbjct:   80 RLIGDAAKNQLTINPENTIFDAKRLIG 106

[Close]

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKKD*L 398
            G  IGI L T YSCVGV +  + EIIANDQ NR TPSYVAF+  + D L
Sbjct:   33 GTIIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFSGDQGDRL 81

HSP 2 Score: 35.8094 bits (81), Expect = 7.034e-14
Identity = 18/27 (66.67%), Postives = 21/27 (77.78%), Query Frame = 2

Query:  389 RLITDAAKNQVRMKCNDTIFYAKRLIG 469
            RLI DAAKNQ+ +   +TIF AKRLIG
Sbjct:   80 RLIGDAAKNQLTINPENTIFDAKRLIG 106

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Fly
Match: Hsp68 (gene:FBgn0001230 transcript:FBtr0084589)

HSP 1 Score: 67.781 bits (164), Expect = 6.504e-18
Identity = 32/44 (72.73%), Postives = 35/44 (79.55%), Query Frame = 3

Query:  258 AIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            AIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    3 AIGIDLGTTYSCVGVFQYGKVEIIANDQGNRTTPSYVAFTDSER 46

HSP 2 Score: 40.0466 bits (92), Expect = 6.504e-18
Identity = 18/32 (56.25%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +++F AKRLIG
Sbjct:   41 FTDSERLIGDAAKNQVAMNPKNSVFDAKRLIG 72

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Fly
Match: Hsc70-4 (gene:FBgn0266599 transcript:FBtr0083059)

HSP 1 Score: 66.6254 bits (161), Expect = 6.662e-18
Identity = 31/44 (70.45%), Postives = 35/44 (79.55%), Query Frame = 3

Query:  258 AIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            A+GI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    6 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 49

HSP 2 Score: 40.817 bits (94), Expect = 6.662e-18
Identity = 20/32 (62.50%), Postives = 22/32 (68.75%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M    TIF AKRLIG
Sbjct:   44 FTDTERLIGDAAKNQVAMNPTQTIFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Fly
Match: Hsc70-4 (gene:FBgn0266599 transcript:FBtr0337053)

HSP 1 Score: 66.6254 bits (161), Expect = 6.662e-18
Identity = 31/44 (70.45%), Postives = 35/44 (79.55%), Query Frame = 3

Query:  258 AIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            A+GI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    6 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 49

HSP 2 Score: 40.817 bits (94), Expect = 6.662e-18
Identity = 20/32 (62.50%), Postives = 22/32 (68.75%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M    TIF AKRLIG
Sbjct:   44 FTDTERLIGDAAKNQVAMNPTQTIFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Fly
Match: Hsc70-4 (gene:FBgn0266599 transcript:FBtr0083058)

HSP 1 Score: 66.6254 bits (161), Expect = 6.662e-18
Identity = 31/44 (70.45%), Postives = 35/44 (79.55%), Query Frame = 3

Query:  258 AIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            A+GI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    6 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 49

HSP 2 Score: 40.817 bits (94), Expect = 6.662e-18
Identity = 20/32 (62.50%), Postives = 22/32 (68.75%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M    TIF AKRLIG
Sbjct:   44 FTDTERLIGDAAKNQVAMNPTQTIFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Fly
Match: Hsc70-4 (gene:FBgn0266599 transcript:FBtr0083060)

HSP 1 Score: 66.6254 bits (161), Expect = 6.662e-18
Identity = 31/44 (70.45%), Postives = 35/44 (79.55%), Query Frame = 3

Query:  258 AIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            A+GI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    6 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 49

HSP 2 Score: 40.817 bits (94), Expect = 6.662e-18
Identity = 20/32 (62.50%), Postives = 22/32 (68.75%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M    TIF AKRLIG
Sbjct:   44 FTDTERLIGDAAKNQVAMNPTQTIFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Zebrafish
Match: hsp70.1 (heat shock cognate 70-kd protein, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-990415-91])

HSP 1 Score: 67.781 bits (164), Expect = 5.868e-19
Identity = 33/46 (71.74%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G AIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    6 GIAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 51

HSP 2 Score: 43.8986 bits (102), Expect = 5.868e-19
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  N+T+F AKRLIG
Sbjct:   46 FTDTERLIGDAAKNQVAMNPNNTVFDAKRLIG 77

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Zebrafish
Match: hsp70.2 (heat shock cognate 70-kd protein, tandem duplicate 2 [Source:ZFIN;Acc:ZDB-GENE-110405-1])

HSP 1 Score: 67.781 bits (164), Expect = 5.868e-19
Identity = 33/46 (71.74%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G AIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    6 GIAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 51

HSP 2 Score: 43.8986 bits (102), Expect = 5.868e-19
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  N+T+F AKRLIG
Sbjct:   46 FTDTERLIGDAAKNQVAMNPNNTVFDAKRLIG 77

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Zebrafish
Match: hsp70.1 (heat shock cognate 70-kd protein, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-990415-91])

HSP 1 Score: 67.781 bits (164), Expect = 7.687e-19
Identity = 33/46 (71.74%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G AIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    6 GIAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 51

HSP 2 Score: 43.5134 bits (101), Expect = 7.687e-19
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  N+T+F AKRLIG
Sbjct:   46 FTDTERLIGDAAKNQVAMNPNNTVFDAKRLIG 77

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Zebrafish
Match: hsp70.2 (heat shock cognate 70-kd protein, tandem duplicate 2 [Source:ZFIN;Acc:ZDB-GENE-110405-1])

HSP 1 Score: 67.781 bits (164), Expect = 7.687e-19
Identity = 33/46 (71.74%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G AIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    6 GIAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 51

HSP 2 Score: 43.5134 bits (101), Expect = 7.687e-19
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  N+T+F AKRLIG
Sbjct:   46 FTDTERLIGDAAKNQVAMNPNNTVFDAKRLIG 77

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Zebrafish
Match: hsp70.3 (heat shock cognate 70-kd protein, tandem duplicate 3 [Source:ZFIN;Acc:ZDB-GENE-110713-1])

HSP 1 Score: 67.781 bits (164), Expect = 7.687e-19
Identity = 33/46 (71.74%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G AIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    6 GIAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 51

HSP 2 Score: 43.5134 bits (101), Expect = 7.687e-19
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  N+T+F AKRLIG
Sbjct:   46 FTDTERLIGDAAKNQVAMNPNNTVFDAKRLIG 77

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Xenopus
Match: wdr26 (WD repeat domain 26 [Source:Xenbase;Acc:XB-GENE-993726])

HSP 1 Score: 70.4774 bits (171), Expect = 8.337e-19
Identity = 34/50 (68.00%), Postives = 38/50 (76.00%), Query Frame = 3

Query:  240 FQMSGNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
             Q SG A+GI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    1 MQPSGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 50

HSP 2 Score: 41.2022 bits (95), Expect = 8.337e-19
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +T+F AKRLIG
Sbjct:   45 FTDTERLIGDAAKNQVAMNPTNTVFDAKRLIG 76

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Xenopus
Match: wdr26 (WD repeat domain 26 [Source:Xenbase;Acc:XB-GENE-993726])

HSP 1 Score: 67.3958 bits (163), Expect = 6.319e-18
Identity = 32/46 (69.57%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G A+GI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    4 GPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 49

HSP 2 Score: 41.2022 bits (95), Expect = 6.319e-18
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +T+F AKRLIG
Sbjct:   44 FTDTERLIGDAAKNQVAMNPTNTVFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Xenopus
Match: ENSXETT00000032295.1 (heat shock protein family A (Hsp70) member 1A [Source:NCBI gene;Acc:100488052])

HSP 1 Score: 67.3958 bits (163), Expect = 6.473e-18
Identity = 32/46 (69.57%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G A+GI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    4 GPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 49

HSP 2 Score: 40.817 bits (94), Expect = 6.473e-18
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +T+F AKRLIG
Sbjct:   44 FTDTERLIGDAAKNQVAMNPTNTVFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Xenopus
Match: wdr26 (WD repeat domain 26 [Source:Xenbase;Acc:XB-GENE-993726])

HSP 1 Score: 67.3958 bits (163), Expect = 6.527e-18
Identity = 32/46 (69.57%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G A+GI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    4 GPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 49

HSP 2 Score: 41.2022 bits (95), Expect = 6.527e-18
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +T+F AKRLIG
Sbjct:   44 FTDTERLIGDAAKNQVAMNPTNTVFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Xenopus
Match: wdr26 (WD repeat domain 26 [Source:Xenbase;Acc:XB-GENE-993726])

HSP 1 Score: 67.3958 bits (163), Expect = 6.634e-18
Identity = 32/46 (69.57%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G A+GI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    9 GPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 54

HSP 2 Score: 40.817 bits (94), Expect = 6.634e-18
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +T+F AKRLIG
Sbjct:   49 FTDTERLIGDAAKNQVAMNPTNTVFDAKRLIG 80

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Mouse
Match: Hspa2 (heat shock protein 2 [Source:MGI Symbol;Acc:MGI:96243])

HSP 1 Score: 68.1662 bits (165), Expect = 2.420e-18
Identity = 33/46 (71.74%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G AIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    5 GPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 50

HSP 2 Score: 41.5874 bits (96), Expect = 2.420e-18
Identity = 20/32 (62.50%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +TIF AKRLIG
Sbjct:   45 FTDTERLIGDAAKNQVAMNPTNTIFDAKRLIG 76

[Close]

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G AIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    5 GPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 50

HSP 2 Score: 41.5874 bits (96), Expect = 2.420e-18
Identity = 20/32 (62.50%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +TIF AKRLIG
Sbjct:   45 FTDTERLIGDAAKNQVAMNPTNTIFDAKRLIG 76

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Mouse
Match: Hspa1l (heat shock protein 1-like [Source:MGI Symbol;Acc:MGI:96231])

HSP 1 Score: 68.1662 bits (165), Expect = 3.019e-18
Identity = 33/46 (71.74%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G AIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    6 GMAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 51

HSP 2 Score: 40.817 bits (94), Expect = 3.019e-18
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +T+F AKRLIG
Sbjct:   46 FTDTERLIGDAAKNQVAMNPQNTVFDAKRLIG 77

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Mouse
Match: Hspa8 (heat shock protein 8 [Source:MGI Symbol;Acc:MGI:105384])

HSP 1 Score: 67.781 bits (164), Expect = 4.585e-18
Identity = 32/46 (69.57%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G A+GI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    4 GPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 49

HSP 2 Score: 40.817 bits (94), Expect = 4.585e-18
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +T+F AKRLIG
Sbjct:   44 FTDTERLIGDAAKNQVAMNPTNTVFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Mouse
Match: Hspa8 (heat shock protein 8 [Source:MGI Symbol;Acc:MGI:105384])

HSP 1 Score: 67.3958 bits (163), Expect = 4.588e-18
Identity = 32/46 (69.57%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G A+GI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    4 GPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 49

HSP 2 Score: 40.817 bits (94), Expect = 4.588e-18
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +T+F AKRLIG
Sbjct:   44 FTDTERLIGDAAKNQVAMNPTNTVFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. UniProt/SwissProt
Match: sp|Q06248|HSP74_PARLI (Heat shock 70 kDa protein IV OS=Paracentrotus lividus OX=7656 GN=HSP70IV PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.839e-18
Identity = 34/47 (72.34%), Postives = 37/47 (78.72%), Query Frame = 3

Query:  249 SGNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            SG AIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    3 SGPAIGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVAFTDSER 49

HSP 2 Score: 42.3578 bits (98), Expect = 2.839e-18
Identity = 20/32 (62.50%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +TIF AKRLIG
Sbjct:   44 FTDSERLIGDAAKNQVAMNPTNTIFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. UniProt/SwissProt
Match: sp|P27541|HSP70_BRUMA (Heat shock 70 kDa protein OS=Brugia malayi OX=6279 GN=HSP70 PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 6.661e-18
Identity = 34/48 (70.83%), Postives = 37/48 (77.08%), Query Frame = 3

Query:  246 MSGNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            MS NAIGI L T YSCVGV    K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    1 MSKNAIGIDLGTTYSCVGVFMHGKVEIIANDQGNRTTPSYVAFTDTER 48

HSP 2 Score: 39.6614 bits (91), Expect = 6.661e-18
Identity = 18/32 (56.25%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  ++T+F A RLIG
Sbjct:   43 FTDTERLIGDAAKNQVAMNPHNTVFDANRLIG 74

[Close]

BLAST of Heat shock 70 kDa protein vs. UniProt/SwissProt
Match: sp|Q91233|HSP70_ONCTS (Heat shock 70 kDa protein OS=Oncorhynchus tshawytscha OX=74940 GN=hsp70 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 7.350e-18
Identity = 32/46 (69.57%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G +IGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    6 GPSIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 51

HSP 2 Score: 43.5134 bits (101), Expect = 7.350e-18
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  N+T+F AKRLIG
Sbjct:   46 FTDTERLIGDAAKNQVAMNPNNTVFDAKRLIG 77

[Close]

BLAST of Heat shock 70 kDa protein vs. UniProt/SwissProt
Match: sp|A5A8V7|HS71L_PIG (Heat shock 70 kDa protein 1-like OS=Sus scrofa OX=9823 GN=HSPA1L PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 9.874e-18
Identity = 33/46 (71.74%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G AIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    6 GTAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 51

HSP 2 Score: 40.817 bits (94), Expect = 9.874e-18
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +T+F AKRLIG
Sbjct:   46 FTDTERLIGDAAKNQVAMNPQNTVFDAKRLIG 77

[Close]

BLAST of Heat shock 70 kDa protein vs. UniProt/SwissProt
Match: sp|P0CB32|HS71L_BOVIN (Heat shock 70 kDa protein 1-like OS=Bos taurus OX=9913 GN=HSPA1L PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 9.955e-18
Identity = 33/46 (71.74%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G AIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    6 GTAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 51

HSP 2 Score: 40.817 bits (94), Expect = 9.955e-18
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +T+F AKRLIG
Sbjct:   46 FTDTERLIGDAAKNQVAMNPQNTVFDAKRLIG 77

[Close]

BLAST of Heat shock 70 kDa protein vs. TrEMBL
Match: A0A023G093 (Putative heat shock protein (Fragment) OS=Amblyomma parvum OX=251391 PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.827e-16
Identity = 40/77 (51.95%), Postives = 46/77 (59.74%), Query Frame = 3

Query:  159 FSSIFEANNVIFEICSCYFIF*LIVD*FQMSGNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            F+ +F   N +  IC C F     V        AIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    5 FARVFGVGNAL-TICWCSFTGMAKVP-------AIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 73

HSP 2 Score: 43.5134 bits (101), Expect = 1.827e-16
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  N+T+F AKRLIG
Sbjct:   68 FTDTERLIGDAAKNQVAMNPNNTVFDAKRLIG 99

[Close]

BLAST of Heat shock 70 kDa protein vs. TrEMBL
Match: A0A3Q0RA61 (Si:ch211-199o1.2 OS=Amphilophus citrinellus OX=61819 PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.859e-16
Identity = 33/48 (68.75%), Postives = 38/48 (79.17%), Query Frame = 3

Query:  246 MSGNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            M+G +IGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    1 MAGISIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 48

HSP 2 Score: 44.2838 bits (103), Expect = 1.859e-16
Identity = 21/32 (65.62%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  N+TIF AKRLIG
Sbjct:   43 FTDTERLIGDAAKNQVAMNPNNTIFDAKRLIG 74

[Close]

BLAST of Heat shock 70 kDa protein vs. TrEMBL
Match: A6YT30 (Heat shock protein 70 OS=Trichinella nativa OX=6335 GN=HSP70 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 2.208e-16
Identity = 35/48 (72.92%), Postives = 38/48 (79.17%), Query Frame = 3

Query:  246 MSGNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            MS NAIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    1 MSKNAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 48

HSP 2 Score: 40.0466 bits (92), Expect = 2.208e-16
Identity = 18/32 (56.25%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV +  ++T+F AKRLIG
Sbjct:   43 FTDTERLIGDAAKNQVALNPHNTVFDAKRLIG 74

[Close]

BLAST of Heat shock 70 kDa protein vs. TrEMBL
Match: A0A5B6Z0H9 (Putative heat shock protein (Fragment) OS=Davidia involucrata OX=16924 GN=Din_006769 PE=3 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.298e-16
Identity = 39/83 (46.99%), Postives = 51/83 (61.45%), Query Frame = 3

Query:  141 FDLNFEFSSIFEANNVIFEICSCYFIF*LIVD*FQMSGNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            F LN+ F+ +     ++FE       F L+    +  G AIGI L T YSCVGV Q ++ EII NDQ NR+TPSYVAFTD ++
Sbjct:   14 FSLNYSFAELQLYFKLVFE-------FKLLPMAAKTEGKAIGIDLGTTYSCVGVWQNDRVEIIPNDQGNRTTPSYVAFTDTER 89

HSP 2 Score: 41.2022 bits (95), Expect = 2.298e-16
Identity = 19/32 (59.38%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  ++T+F AKRLIG
Sbjct:   84 FTDTERLIGDAAKNQVAMNPSNTVFDAKRLIG 115

[Close]

BLAST of Heat shock 70 kDa protein vs. TrEMBL
Match: A0A3M7SUB5 (Heat shock 70 kDa cognate 4 OS=Brachionus plicatilis OX=10195 GN=BpHYR1_005077 PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 2.300e-16
Identity = 32/46 (69.57%), Postives = 37/46 (80.43%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G+AIGI L T YSCVG+ Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    4 GSAIGIDLGTTYSCVGIFQHGKVEIIANDQGNRTTPSYVAFTDSER 49

HSP 2 Score: 43.8986 bits (102), Expect = 2.300e-16
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  N+T+F AKRLIG
Sbjct:   44 FTDSERLIGDAAKNQVAMNPNNTVFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Cavefish
Match: ENSAMXT00000032722.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001455.1:155723:156505:1 gene:ENSAMXG00000042801.1 transcript:ENSAMXT00000032722.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 68.1662 bits (165), Expect = 3.189e-19
Identity = 33/46 (71.74%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G AIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    6 GVAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 51

HSP 2 Score: 43.8986 bits (102), Expect = 3.189e-19
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  N+T+F AKRLIG
Sbjct:   46 FTDTERLIGDAAKNQVAMNPNNTVFDAKRLIG 77

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Cavefish
Match: ENSAMXT00000055474.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001455.1:150188:156169:-1 gene:ENSAMXG00000043809.1 transcript:ENSAMXT00000055474.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 67.781 bits (164), Expect = 5.190e-19
Identity = 33/46 (71.74%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G AIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    6 GVAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 51

HSP 2 Score: 43.5134 bits (101), Expect = 5.190e-19
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  N+T+F AKRLIG
Sbjct:   46 FTDTERLIGDAAKNQVAMNPNNTVFDAKRLIG 77

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Cavefish
Match: ENSAMXT00000031477.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001455.1:148482:152871:-1 gene:ENSAMXG00000043809.1 transcript:ENSAMXT00000031477.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 67.781 bits (164), Expect = 5.190e-19
Identity = 33/46 (71.74%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G AIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    6 GVAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 51

HSP 2 Score: 43.5134 bits (101), Expect = 5.190e-19
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  N+T+F AKRLIG
Sbjct:   46 FTDTERLIGDAAKNQVAMNPNNTVFDAKRLIG 77

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Cavefish
Match: hspa1b (heat shock protein family A (Hsp70) member 1B [Source:ZFIN;Acc:ZDB-GENE-060503-867])

HSP 1 Score: 67.0106 bits (162), Expect = 7.814e-19
Identity = 32/47 (68.09%), Postives = 37/47 (78.72%), Query Frame = 3

Query:  249 SGNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            +G +IGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    3 AGVSIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 49

HSP 2 Score: 43.8986 bits (102), Expect = 7.814e-19
Identity = 21/28 (75.00%), Postives = 23/28 (82.14%), Query Frame = 2

Query:  386 KRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +RLI DAAKNQV M  N+TIF AKRLIG
Sbjct:   48 ERLIGDAAKNQVAMNPNNTIFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Cavefish
Match: hsc70 (heat shock cognate 71 kDa protein-like [Source:NCBI gene;Acc:103045816])

HSP 1 Score: 67.0106 bits (162), Expect = 1.049e-18
Identity = 31/46 (67.39%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G A+GI L T YSCVG+ Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    4 GPAVGIDLGTTYSCVGIFQHGKVEIIANDQGNRTTPSYVAFTDTER 49

HSP 2 Score: 43.5134 bits (101), Expect = 1.049e-18
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  N+T+F AKRLIG
Sbjct:   44 FTDTERLIGDAAKNQVAMNPNNTVFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Sea Lamprey
Match: hspa5 (heat shock protein 5 [Source:NCBI gene;Acc:378848])

HSP 1 Score: 59.6918 bits (143), Expect = 1.747e-14
Identity = 29/44 (65.91%), Postives = 32/44 (72.73%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQ 383
            G  IGI L T YSCVGV +  + EIIANDQ NR TPSYVAFT +
Sbjct:   33 GTVIGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTAE 76

HSP 2 Score: 34.2686 bits (77), Expect = 1.747e-14
Identity = 17/28 (60.71%), Postives = 21/28 (75.00%), Query Frame = 2

Query:  386 KRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +RLI DAAKNQ+     +T+F AKRLIG
Sbjct:   78 ERLIGDAAKNQLTSNPENTVFDAKRLIG 105

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Sea Lamprey
Match: hspa9 (heat shock protein 9 [Source:ZFIN;Acc:ZDB-GENE-030828-12])

HSP 1 Score: 40.4318 bits (93), Expect = 6.812e-6
Identity = 19/44 (43.18%), Postives = 29/44 (65.91%), Query Frame = 3

Query:  246 MSGNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFT 377
            + G  IGI L T  SCV V++ ++ +++ N +  R+TPS VAFT
Sbjct:   51 IRGAVIGIDLGTTNSCVAVMEGKQAKVLENAEGARTTPSVVAFT 94

HSP 2 Score: 24.2534 bits (51), Expect = 6.812e-6
Identity = 12/28 (42.86%), Postives = 16/28 (57.14%), Query Frame = 2

Query:  386 KRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +RL+   AK Q     N+T +  KRLIG
Sbjct:   98 ERLVGMPAKRQAVTNPNNTFYATKRLIG 125

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Yeast
Match: KAR2 (ATPase involved in protein import into the ER; also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p [Source:SGD;Acc:S000003571])

HSP 1 Score: 60.077 bits (144), Expect = 1.641e-15
Identity = 28/46 (60.87%), Postives = 34/46 (73.91%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G  IGI L T YSCV V++  K EI+AN+Q NR TPSYVAFTD ++
Sbjct:   50 GTVIGIDLGTTYSCVAVMKNGKTEILANEQGNRITPSYVAFTDDER 95

HSP 2 Score: 36.965 bits (84), Expect = 1.641e-15
Identity = 18/33 (54.55%), Postives = 23/33 (69.70%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIGM 472
            +  ++RLI DAAKNQV     +TIF  KRLIG+
Sbjct:   90 FTDDERLIGDAAKNQVAANPQNTIFDIKRLIGL 122

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Yeast
Match: SSA4 (Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication [Source:SGD;Acc:S000000905])

HSP 1 Score: 59.6918 bits (143), Expect = 2.100e-15
Identity = 27/44 (61.36%), Postives = 33/44 (75.00%), Query Frame = 3

Query:  258 AIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            A+GI L T YSCV     ++ EIIANDQ NR+TPSYVAFTD ++
Sbjct:    4 AVGIDLGTTYSCVAHFANDRVEIIANDQGNRTTPSYVAFTDTER 47

HSP 2 Score: 36.965 bits (84), Expect = 2.100e-15
Identity = 18/32 (56.25%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQ  M  ++T+F AKRLIG
Sbjct:   42 FTDTERLIGDAAKNQAAMNPHNTVFDAKRLIG 73

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Yeast
Match: SSA3 (ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication [Source:SGD;Acc:S000000171])

HSP 1 Score: 60.077 bits (144), Expect = 5.640e-15
Identity = 27/44 (61.36%), Postives = 33/44 (75.00%), Query Frame = 3

Query:  258 AIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            A+GI L T YSCV     ++ EIIANDQ NR+TPSYVAFTD ++
Sbjct:    4 AVGIDLGTTYSCVAHFSNDRVEIIANDQGNRTTPSYVAFTDTER 47

HSP 2 Score: 35.039 bits (79), Expect = 5.640e-15
Identity = 17/32 (53.12%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQ  +  ++T+F AKRLIG
Sbjct:   42 FTDTERLIGDAAKNQAAINPHNTVFDAKRLIG 73

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Yeast
Match: SSA1 (ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; required for ubiquitin-dependent degradation of short-lived proteins; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, cell wall; 98% identical to paralog Ssa2p with different functional specificity in propagation of yeast [URE3] prions, vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils [Source:SGD;Acc:S000000004])

HSP 1 Score: 56.9954 bits (136), Expect = 1.431e-14
Identity = 25/44 (56.82%), Postives = 33/44 (75.00%), Query Frame = 3

Query:  258 AIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            A+GI L T YSCV     ++ +IIANDQ NR+TPS+VAFTD ++
Sbjct:    4 AVGIDLGTTYSCVAHFANDRVDIIANDQGNRTTPSFVAFTDTER 47

HSP 2 Score: 36.965 bits (84), Expect = 1.431e-14
Identity = 18/32 (56.25%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQ  M  ++T+F AKRLIG
Sbjct:   42 FTDTERLIGDAAKNQAAMNPSNTVFDAKRLIG 73

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Yeast
Match: SSA2 (HSP70 family ATP-binding protein; involved in protein folding, vacuolar import of proteins; required for ubiquitin-dependent degradation of short-lived proteins; associated with chaperonin-containing T-complex; 98% identical to paralog Ssa1p with distinct functional specificity in propagation of yeast [URE3] prions and vacuolar-mediated degradation of gluconeogenesis enzymes; binds tRNA, has role in tRNA nuclear import during starvation [Source:SGD;Acc:S000003947])

HSP 1 Score: 55.4546 bits (132), Expect = 4.826e-14
Identity = 24/44 (54.55%), Postives = 32/44 (72.73%), Query Frame = 3

Query:  258 AIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            A+GI L T YSCV     ++ +IIANDQ NR+TPS+V FTD ++
Sbjct:    4 AVGIDLGTTYSCVAHFSNDRVDIIANDQGNRTTPSFVGFTDTER 47

HSP 2 Score: 36.5798 bits (83), Expect = 4.826e-14
Identity = 18/32 (56.25%), Postives = 22/32 (68.75%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQ  M   +T+F AKRLIG
Sbjct:   42 FTDTERLIGDAAKNQAAMNPANTVFDAKRLIG 73

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Nematostella
Match: EDO30323 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T144])

HSP 1 Score: 68.5514 bits (166), Expect = 1.166e-19
Identity = 32/44 (72.73%), Postives = 36/44 (81.82%), Query Frame = 3

Query:  258 AIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            AIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD+++
Sbjct:    6 AIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDEER 49

HSP 2 Score: 43.5134 bits (101), Expect = 1.166e-19
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +  E+RL+ DAAKNQV M   +TIF AKRLIG
Sbjct:   44 FTDEERLVGDAAKNQVAMNPTNTIFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Nematostella
Match: EDO37280 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SG65])

HSP 1 Score: 65.0846 bits (157), Expect = 5.100e-18
Identity = 30/44 (68.18%), Postives = 35/44 (79.55%), Query Frame = 3

Query:  258 AIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            A+GI L T YSCVGV Q  K EIIANDQ NR+TPSYVAF+D ++
Sbjct:    6 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFSDTER 49

HSP 2 Score: 41.5874 bits (96), Expect = 5.100e-18
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +T+F AKRLIG
Sbjct:   44 FSDTERLIGDAAKNQVAMNPENTVFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Nematostella
Match: EDO44530 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RV88])

HSP 1 Score: 65.4698 bits (158), Expect = 8.324e-17
Identity = 31/44 (70.45%), Postives = 34/44 (77.27%), Query Frame = 3

Query:  258 AIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            AIGI L T YSCVGV Q  K EIIANDQ NR+TPSYV FTD ++
Sbjct:    6 AIGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVGFTDTER 49

HSP 2 Score: 36.965 bits (84), Expect = 8.324e-17
Identity = 18/32 (56.25%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RL+ DAAKNQV +   +TIF AKRLIG
Sbjct:   44 FTDTERLVGDAAKNQVALNPENTIFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Nematostella
Match: EDO33296 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSL5])

HSP 1 Score: 59.6918 bits (143), Expect = 7.702e-14
Identity = 29/44 (65.91%), Postives = 33/44 (75.00%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQ 383
            G  IGI L T YSCVGV +  + EIIANDQ NR TPSYVAFT++
Sbjct:   33 GTVIGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTEE 76

HSP 2 Score: 32.7278 bits (73), Expect = 7.702e-14
Identity = 16/30 (53.33%), Postives = 21/30 (70.00%), Query Frame = 2

Query:  386 KRLITDAAKNQVRMKCNDTIFYAKRLIGMT 475
            +RLI D AKNQ+     +T+F AKRLIG +
Sbjct:   78 ERLIGDGAKNQLTTNPENTVFDAKRLIGRS 107

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Medaka
Match: hspa8b (heat shock cognate 70 kDa protein [Source:NCBI gene;Acc:100125490])

HSP 1 Score: 67.3958 bits (163), Expect = 2.007e-18
Identity = 32/46 (69.57%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G A+GI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    4 GPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 49

HSP 2 Score: 41.9726 bits (97), Expect = 2.007e-18
Identity = 19/32 (59.38%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV +  N+T+F AKRLIG
Sbjct:   44 FTDTERLIGDAAKNQVALNPNNTVFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Medaka
Match: hspa8b (heat shock cognate 70 kDa protein [Source:NCBI gene;Acc:100125490])

HSP 1 Score: 67.3958 bits (163), Expect = 2.289e-18
Identity = 32/46 (69.57%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G A+GI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    4 GPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 49

HSP 2 Score: 41.9726 bits (97), Expect = 2.289e-18
Identity = 19/32 (59.38%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV +  N+T+F AKRLIG
Sbjct:   44 FTDTERLIGDAAKNQVALNPNNTVFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Medaka
Match: hsc70 (heat shock protein 70 cognate [Source:NCBI gene;Acc:100049425])

HSP 1 Score: 67.3958 bits (163), Expect = 4.699e-18
Identity = 32/46 (69.57%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G A+GI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:   54 GPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 99

HSP 2 Score: 40.817 bits (94), Expect = 4.699e-18
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +T+F AKRLIG
Sbjct:   94 FTDTERLIGDAAKNQVAMNPTNTVFDAKRLIG 125

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Medaka
Match: hsc70 (heat shock protein 70 cognate [Source:NCBI gene;Acc:100049425])

HSP 1 Score: 67.3958 bits (163), Expect = 4.785e-18
Identity = 32/46 (69.57%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G A+GI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:   54 GPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 99

HSP 2 Score: 40.817 bits (94), Expect = 4.785e-18
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +T+F AKRLIG
Sbjct:   94 FTDTERLIGDAAKNQVAMNPTNTVFDAKRLIG 125

[Close]

BLAST of Heat shock 70 kDa protein vs. Ensembl Medaka
Match: hsp70-3 (heat shock protein 70 [Source:NCBI gene;Acc:100125493])

HSP 1 Score: 67.0106 bits (162), Expect = 7.690e-18
Identity = 31/46 (67.39%), Postives = 36/46 (78.26%), Query Frame = 3

Query:  252 GNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            G A+GI L T YSCVG+ Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    4 GPAVGIDLGTTYSCVGIFQHGKVEIIANDQGNRTTPSYVAFTDTER 49

HSP 2 Score: 40.4318 bits (93), Expect = 7.690e-18
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M   +T+F AKRLIG
Sbjct:   44 FTDTERLIGDAAKNQVAMNPANTVFDAKRLIG 75

[Close]

BLAST of Heat shock 70 kDa protein vs. Planmine SMEST
Match: SMESG000051570.1 (SMESG000051570.1)

HSP 1 Score: 75.8702 bits (185), Expect = 2.149e-21
Identity = 36/48 (75.00%), Postives = 39/48 (81.25%), Query Frame = 3

Query:  246 MSGNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            MSGNAIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    1 MSGNAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 48

HSP 2 Score: 43.5134 bits (101), Expect = 2.149e-21
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  N+T+F AKRLIG
Sbjct:   43 FTDTERLIGDAAKNQVAMNPNNTVFDAKRLIG 74

[Close]

BLAST of Heat shock 70 kDa protein vs. Planmine SMEST
Match: SMESG000051567.1 (SMESG000051567.1)

HSP 1 Score: 75.8702 bits (185), Expect = 2.149e-21
Identity = 36/48 (75.00%), Postives = 39/48 (81.25%), Query Frame = 3

Query:  246 MSGNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            MSGNAIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    1 MSGNAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 48

HSP 2 Score: 43.5134 bits (101), Expect = 2.149e-21
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  N+T+F AKRLIG
Sbjct:   43 FTDTERLIGDAAKNQVAMNPNNTVFDAKRLIG 74

[Close]

BLAST of Heat shock 70 kDa protein vs. Planmine SMEST
Match: SMESG000051541.1 (SMESG000051541.1)

HSP 1 Score: 75.8702 bits (185), Expect = 2.149e-21
Identity = 36/48 (75.00%), Postives = 39/48 (81.25%), Query Frame = 3

Query:  246 MSGNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            MSGNAIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    1 MSGNAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 48

HSP 2 Score: 43.5134 bits (101), Expect = 2.149e-21
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  N+T+F AKRLIG
Sbjct:   43 FTDTERLIGDAAKNQVAMNPNNTVFDAKRLIG 74

[Close]

BLAST of Heat shock 70 kDa protein vs. Planmine SMEST
Match: SMESG000051540.1 (SMESG000051540.1)

HSP 1 Score: 75.8702 bits (185), Expect = 2.149e-21
Identity = 36/48 (75.00%), Postives = 39/48 (81.25%), Query Frame = 3

Query:  246 MSGNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            MSGNAIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    1 MSGNAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 48

HSP 2 Score: 43.5134 bits (101), Expect = 2.149e-21
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  374 YRSEKRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +   +RLI DAAKNQV M  N+T+F AKRLIG
Sbjct:   43 FTDTERLIGDAAKNQVAMNPNNTVFDAKRLIG 74

[Close]

BLAST of Heat shock 70 kDa protein vs. Planmine SMEST
Match: SMESG000051815.1 (SMESG000051815.1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.672e-20
Identity = 35/48 (72.92%), Postives = 38/48 (79.17%), Query Frame = 3

Query:  246 MSGNAIGIGLSTAYSCVGVIQLEKPEIIANDQENRSTPSYVAFTDQKK 389
            MS NAIGI L T YSCVGV Q  K EIIANDQ NR+TPSYVAFTD ++
Sbjct:    1 MSVNAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER 48

HSP 2 Score: 43.5134 bits (101), Expect = 2.672e-20
Identity = 20/28 (71.43%), Postives = 23/28 (82.14%), Query Frame = 2

Query:  386 KRLITDAAKNQVRMKCNDTIFYAKRLIG 469
            +RLI DAAKNQV M  N+T+F AKRLIG
Sbjct:   47 ERLIGDAAKNQVAMNPNNTVFDAKRLIG 74

The following BLAST results are available for this feature:

BLAST of Heat shock 70 kDa protein vs. Ensembl Human
BLAST of Heat shock 70 kDa protein vs. Ensembl Celegans
BLAST of Heat shock 70 kDa protein vs. Ensembl Fly
BLAST of Heat shock 70 kDa protein vs. Ensembl Zebrafish
BLAST of Heat shock 70 kDa protein vs. Ensembl Xenopus
BLAST of Heat shock 70 kDa protein vs. Ensembl Mouse
BLAST of Heat shock 70 kDa protein vs. UniProt/SwissProt
BLAST of Heat shock 70 kDa protein vs. TrEMBL
BLAST of Heat shock 70 kDa protein vs. Ensembl Cavefish
BLAST of Heat shock 70 kDa protein vs. Ensembl Sea Lamprey
BLAST of Heat shock 70 kDa protein vs. Ensembl Yeast
BLAST of Heat shock 70 kDa protein vs. Ensembl Nematostella
BLAST of Heat shock 70 kDa protein vs. Ensembl Medaka
BLAST of Heat shock 70 kDa protein vs. Planmine SMEST

BLAST of Heat shock 70 kDa protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
HSPA2	3.274e-18	71.74	heat shock protein family A (Hsp70) member 2 [Sour...	[more]
HSPA2	3.274e-18	71.74	heat shock protein family A (Hsp70) member 2 [Sour...	[more]
HSPA8	4.607e-18	69.57	heat shock protein family A (Hsp70) member 8 [Sour...	[more]
HSPA8	4.895e-18	69.57	heat shock protein family A (Hsp70) member 8 [Sour...	[more]
HSPA8	5.053e-18	69.57	heat shock protein family A (Hsp70) member 8 [Sour...	[more]

BLAST of Heat shock 70 kDa protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
hsp-1	3.032e-18	68.89	Heat shock 70 kDa protein A [Source:UniProtKB/Swi...	[more]
hsp-1	3.032e-18	68.89	Heat shock 70 kDa protein A [Source:UniProtKB/Swi...	[more]
hsp-3	7.034e-14	61.22	Heat shock 70 kDa protein C [Source:UniProtKB/Swi...	[more]
hsp-3	7.034e-14	61.22	Heat shock 70 kDa protein C [Source:UniProtKB/Swi...	[more]
hsp-3	7.034e-14	61.22	Heat shock 70 kDa protein C [Source:UniProtKB/Swi...	[more]

BLAST of Heat shock 70 kDa protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Hsp68	6.504e-18	72.73	gene:FBgn0001230 transcript:FBtr0084589	[more]
Hsc70-4	6.662e-18	70.45	gene:FBgn0266599 transcript:FBtr0083059	[more]
Hsc70-4	6.662e-18	70.45	gene:FBgn0266599 transcript:FBtr0337053	[more]
Hsc70-4	6.662e-18	70.45	gene:FBgn0266599 transcript:FBtr0083058	[more]
Hsc70-4	6.662e-18	70.45	gene:FBgn0266599 transcript:FBtr0083060	[more]

BLAST of Heat shock 70 kDa protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
hsp70.1	5.868e-19	71.74	heat shock cognate 70-kd protein, tandem duplicate...	[more]
hsp70.2	5.868e-19	71.74	heat shock cognate 70-kd protein, tandem duplicate...	[more]
hsp70.1	7.687e-19	71.74	heat shock cognate 70-kd protein, tandem duplicate...	[more]
hsp70.2	7.687e-19	71.74	heat shock cognate 70-kd protein, tandem duplicate...	[more]
hsp70.3	7.687e-19	71.74	heat shock cognate 70-kd protein, tandem duplicate...	[more]

BLAST of Heat shock 70 kDa protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
wdr26	8.337e-19	68.00	WD repeat domain 26 [Source:Xenbase;Acc:XB-GENE-99...	[more]
wdr26	6.319e-18	69.57	WD repeat domain 26 [Source:Xenbase;Acc:XB-GENE-99...	[more]
ENSXETT00000032295.1	6.473e-18	69.57	heat shock protein family A (Hsp70) member 1A [Sou...	[more]
wdr26	6.527e-18	69.57	WD repeat domain 26 [Source:Xenbase;Acc:XB-GENE-99...	[more]
wdr26	6.634e-18	69.57	WD repeat domain 26 [Source:Xenbase;Acc:XB-GENE-99...	[more]

BLAST of Heat shock 70 kDa protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Hspa2	2.420e-18	71.74	heat shock protein 2 [Source:MGI Symbol;Acc:MGI:96...	[more]
Hspa2	2.420e-18	71.74	heat shock protein 2 [Source:MGI Symbol;Acc:MGI:96...	[more]
Hspa1l	3.019e-18	71.74	heat shock protein 1-like [Source:MGI Symbol;Acc:M...	[more]
Hspa8	4.585e-18	69.57	heat shock protein 8 [Source:MGI Symbol;Acc:MGI:10...	[more]
Hspa8	4.588e-18	69.57	heat shock protein 8 [Source:MGI Symbol;Acc:MGI:10...	[more]

BLAST of Heat shock 70 kDa protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q06248\|HSP74_PARLI	2.839e-18	72.34	Heat shock 70 kDa protein IV OS=Paracentrotus livi...	[more]
sp\|P27541\|HSP70_BRUMA	6.661e-18	70.83	Heat shock 70 kDa protein OS=Brugia malayi OX=6279...	[more]
sp\|Q91233\|HSP70_ONCTS	7.350e-18	69.57	Heat shock 70 kDa protein OS=Oncorhynchus tshawyts...	[more]
sp\|A5A8V7\|HS71L_PIG	9.874e-18	71.74	Heat shock 70 kDa protein 1-like OS=Sus scrofa OX=...	[more]
sp\|P0CB32\|HS71L_BOVIN	9.955e-18	71.74	Heat shock 70 kDa protein 1-like OS=Bos taurus OX=...	[more]

BLAST of Heat shock 70 kDa protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A023G093	1.827e-16	51.95	Putative heat shock protein (Fragment) OS=Amblyomm...	[more]
A0A3Q0RA61	1.859e-16	68.75	Si:ch211-199o1.2 OS=Amphilophus citrinellus OX=618...	[more]
A6YT30	2.208e-16	72.92	Heat shock protein 70 OS=Trichinella nativa OX=633...	[more]
A0A5B6Z0H9	2.298e-16	46.99	Putative heat shock protein (Fragment) OS=Davidia ...	[more]
A0A3M7SUB5	2.300e-16	69.57	Heat shock 70 kDa cognate 4 OS=Brachionus plicatil...	[more]

BLAST of Heat shock 70 kDa protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSAMXT00000032722.1	3.189e-19	71.74	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]
ENSAMXT00000055474.1	5.190e-19	71.74	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]
ENSAMXT00000031477.1	5.190e-19	71.74	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]
hspa1b	7.814e-19	68.09	heat shock protein family A (Hsp70) member 1B [Sou...	[more]
hsc70	1.049e-18	67.39	heat shock cognate 71 kDa protein-like [Source:NCB...	[more]

BLAST of Heat shock 70 kDa protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2


Match Name	E-value	Identity	Description
hspa5	1.747e-14	65.91	heat shock protein 5 [Source:NCBI gene;Acc:378848]	[more]
hspa9	6.812e-6	43.18	heat shock protein 9 [Source:ZFIN;Acc:ZDB-GENE-030...	[more]

BLAST of Heat shock 70 kDa protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5


Match Name	E-value	Identity	Description
KAR2	1.641e-15	60.87	ATPase involved in protein import into the ER; als...	[more]
SSA4	2.100e-15	61.36	Heat shock protein that is highly induced upon str...	[more]
SSA3	5.640e-15	61.36	ATPase involved in protein folding and the respons...	[more]
SSA1	1.431e-14	56.82	ATPase involved in protein folding and NLS-directe...	[more]
SSA2	4.826e-14	54.55	HSP70 family ATP-binding protein; involved in prot...	[more]

BLAST of Heat shock 70 kDa protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 4


Match Name	E-value	Identity	Description
EDO30323	1.166e-19	72.73	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO37280	5.100e-18	68.18	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO44530	8.324e-17	70.45	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO33296	7.702e-14	65.91	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Heat shock 70 kDa protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
hspa8b	2.007e-18	69.57	heat shock cognate 70 kDa protein [Source:NCBI gen...	[more]
hspa8b	2.289e-18	69.57	heat shock cognate 70 kDa protein [Source:NCBI gen...	[more]
hsc70	4.699e-18	69.57	heat shock protein 70 cognate [Source:NCBI gene;Ac...	[more]
hsc70	4.785e-18	69.57	heat shock protein 70 cognate [Source:NCBI gene;Ac...	[more]
hsp70-3	7.690e-18	67.39	heat shock protein 70 [Source:NCBI gene;Acc:100125...	[more]

BLAST of Heat shock 70 kDa protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000051570.1	2.149e-21	75.00	SMESG000051570.1	[more]
SMESG000051567.1	2.149e-21	75.00	SMESG000051567.1	[more]
SMESG000051541.1	2.149e-21	75.00	SMESG000051541.1	[more]
SMESG000051540.1	2.149e-21	75.00	SMESG000051540.1	[more]
SMESG000051815.1	2.672e-20	72.92	SMESG000051815.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence

transcript sequence

>SMED30015695 ID=SMED30015695|Name=Heat shock 70 kDa protein|organism=Schmidtea mediterranea sexual|type=transcript|length=482bp
GATGTATTGCTGGCTGTGATACCACCATAGGGATTGCAGGATAATAATAA
GGTTATTCAATCTCAGAATCCAAATCAAGCTCTATTGATTTCTGTTTGGT
CTTGATGTGCTGTACTGGGTGCATAATTATGCCGGATATTTTTGATCTGA
ATTTTGAATTTTCAAGCATTTTCGAAGCAAATAACGTAATTTTTGAAATT
TGCTCTTGCTATTTTATTTTTTAACTTATTGTCGATTGATTCCAAATGTC
TGGTAACGCTATCGGTATTGGGTTGAGTACTGCCTATTCATGCGTTGGTG
TAATTCAACTTGAAAAACCTGAAATTATTGCTAATGATCAAGAAAACCGC
TCAACGCCCAGTTATGTTGCATTTACAGATCAGAAAAAAGATTGATTACG
GATGCTGCAAAAAATCAAGTTAGAATGAAGTGTAACGATACGATTTTCTA
TGCCAAACGACTAATCGGAATGACTGATAAAA

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000070	intestinal phagocyte

Vocabulary: **INTERPRO**
Term	Definition
IPR043129	ATPase_NBD
IPR013126	Hsp_70_fam

Vocabulary: **molecular function**
Term	Definition
GO:0000166	nucleotide binding
GO:0005524	ATP binding

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00301	HEATSHOCK70	coord: 32..44 score: 71.39 coord: 4..17 score: 61.65
None	No IPR available	GENE3D	G3DSA:3.30.420.40		coord: 1..48 e-value: 1.7E-13 score: 52.3
None	No IPR available	PANTHER	PTHR19375:SF376		coord: 2..47
IPR013126	Heat shock protein 70 family	PFAM	PF00012	HSP70	coord: 5..47 e-value: 1.1E-12 score: 46.9
IPR013126	Heat shock protein 70 family	PANTHER	PTHR19375	HEAT SHOCK PROTEIN 70KDA	coord: 2..47
IPR043129	ATPase, nucleotide binding domain	SUPERFAMILY	SSF53067	Actin-like ATPase domain	coord: 4..46

Main menu

Heat shock 70 kDa protein

Zeng et. al., 2018

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 2

Owlery

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