Thioredoxin domain-containing protein

Overview
NameThioredoxin domain-containing protein
Smed IDSMED30015682
Length (bp)1509
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Thioredoxin domain-containing protein (SMED30015682) t-SNE clustered cells

Violin plots show distribution of expression levels for Thioredoxin domain-containing protein (SMED30015682) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Thioredoxin domain-containing protein (SMED30015682) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Thioredoxin domain-containing protein (SMED30015682) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30015682

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
neoblastSMED30015682h1SMcG0017877 dd_Smed_v4_6362_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior sideSMED30015682h1SMcG0017877 dd_Smed_v4_6362_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30015682h1SMcG0017877 dd_Smed_v4_6362_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30015682h1SMcG0017877 Contig55565newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Thioredoxin domain-containing protein vs. Ensembl Human
Match: TXNDC15 (thioredoxin domain containing 15 [Source:HGNC Symbol;Acc:HGNC:20652])

HSP 1 Score: 86.2705 bits (212), Expect = 7.531e-18
Identity = 45/130 (34.62%), Postives = 68/130 (52.31%), Query Frame = 3 
Query:  501 CTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASNFWLIISWILTIICVLY 890
            CT +  Y  WCRFS + AP FN+L R FP L F+AL  +          T  +P I ++ G+K +  FN +D  L+ L  FI+  TG     +  +T+ D  GP+PS L  + ++ L+ S    I  ++Y
Sbjct:  194 CTLVLFYTPWCRFSASLAPHFNSLPRAFPALHFLALDASQHSSLSTRFGTVAVPNILLFQGAKPMARFNHTDRTLETLKIFIFNQTGIEAKKNVVVTQADQIGPLPSTLIKSVDWLLVFSLFFLISFIMY 323
[Close]
BLAST of Thioredoxin domain-containing protein vs. Ensembl Human
Match: TXNDC15 (thioredoxin domain containing 15 [Source:HGNC Symbol;Acc:HGNC:20652])

HSP 1 Score: 86.2705 bits (212), Expect = 1.072e-17
Identity = 45/130 (34.62%), Postives = 68/130 (52.31%), Query Frame = 3 
Query:  501 CTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASNFWLIISWILTIICVLY 890
            CT +  Y  WCRFS + AP FN+L R FP L F+AL  +          T  +P I ++ G+K +  FN +D  L+ L  FI+  TG     +  +T+ D  GP+PS L  + ++ L+ S    I  ++Y
Sbjct:  210 CTLVLFYTPWCRFSASLAPHFNSLPRAFPALHFLALDASQHSSLSTRFGTVAVPNILLFQGAKPMARFNHTDRTLETLKIFIFNQTGIEAKKNVVVTQADQIGPLPSTLIKSVDWLLVFSLFFLISFIMY 339
[Close]
BLAST of Thioredoxin domain-containing protein vs. Ensembl Zebrafish
Match: txndc15 (thioredoxin domain containing 15 [Source:ZFIN;Acc:ZDB-GENE-070615-36])

HSP 1 Score: 77.7962 bits (190), Expect = 1.762e-15
Identity = 40/127 (31.50%), Postives = 64/127 (50.39%), Query Frame = 3 
Query:  498 KCTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASNFWLIISWILTII 878
            +C+ +  Y  WC FS + AP FNAL R FP + F+AL  +          T  +P I ++ G K +  FN ++  L+ L  FI   TG    +   +  +D +GP+P+ +   S  WL +  +L I+
Sbjct:  135 ECSLVLFYTGWCPFSASLAPHFNALPRAFPSMHFLALDASQHSSLSTRFGTVAVPNILLFQGVKPMARFNQTERTLETLTAFISNQTGFEASVGQCVLPEDHAGPLPA-VPVRSIDWLFVFSVLFIL 260
[Close]
BLAST of Thioredoxin domain-containing protein vs. Ensembl Xenopus
Match: TXNDC15 (thioredoxin domain containing 15 [Source:NCBI gene;Acc:100379756])

HSP 1 Score: 89.7373 bits (221), Expect = 4.906e-19
Identity = 46/140 (32.86%), Postives = 68/140 (48.57%), Query Frame = 3 
Query:  498 KCTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASNFWLIISWILTIICVLYLIATTGRGR 917
            +CT +  Y  WCRFS   AP FN L R FP L F+AL  +          T  +P I ++ G+K +  FN +D  L+AL  FI+  +G   +    +T+DD   P+P       ++ L+ S I   I ++Y    T   R
Sbjct:  187 ECTLVLFYTPWCRFSAGLAPHFNTLPRAFPTLHFLALDASQHSSLSTRFGTVAVPNILLFQGAKPMARFNHTDRKLEALKSFIFNQSGIEAVPGVVVTEDDCESPLPCAPTVGIDWLLVFSVIYVTIFIVYATMQTESIR 326
[Close]
BLAST of Thioredoxin domain-containing protein vs. Ensembl Mouse
Match: Txndc15 (thioredoxin domain containing 15 [Source:MGI Symbol;Acc:MGI:1916922])

HSP 1 Score: 84.7297 bits (208), Expect = 1.664e-17
Identity = 45/130 (34.62%), Postives = 67/130 (51.54%), Query Frame = 3 
Query:  501 CTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASNFWLIISWILTIICVLY 890
            CT +  Y  WCRFS + AP FN+L R FP L F+AL  +          T  +P I ++ G+K +  FN +D  L+ L  FI+  TG     +  +T+ D  GP+PS L    ++ L+ S    I  ++Y
Sbjct:  194 CTLVLFYTPWCRFSASLAPHFNSLPRAFPTLGFLALDASQHSSLSTRFGTVAVPNILLFQGAKPMARFNHTDRTLETLKIFIFNQTGIEAKKNVVVTQADQMGPLPSTLIKTVDWLLVFSLFFLISFIMY 323
[Close]
BLAST of Thioredoxin domain-containing protein vs. UniProt/SwissProt
Match: sp|Q0IHI1|TXD15_XENLA (Thioredoxin domain-containing protein 15 OS=Xenopus laevis OX=8355 GN=txndc15 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.775e-18
Identity = 46/140 (32.86%), Postives = 68/140 (48.57%), Query Frame = 3 
Query:  498 KCTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASNFWLIISWILTIICVLYLIATTGRGR 917
            +CT +  Y  WCRFS   AP FN L R FP L F+AL  +          T  +P I ++ G+K +  FN +D  L+AL  FI+  +G   +    +T+DD   P+P       ++ L+ S I   I ++Y    T   R
Sbjct:  191 ECTLVLFYTPWCRFSAGLAPHFNTLPRAFPTLHFLALDASQHSSLSTRFGTVAVPNILLFQGAKPMARFNHTDRKLEALKSFIFNQSGIEAVPGVVVTEDDCESPLPCAPTVGIDWLLVFSVIYVTIFIVYATMQTESIR 330
[Close]
BLAST of Thioredoxin domain-containing protein vs. UniProt/SwissProt
Match: sp|Q96J42|TXD15_HUMAN (Thioredoxin domain-containing protein 15 OS=Homo sapiens OX=9606 GN=TXNDC15 PE=1 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 5.149e-17
Identity = 45/130 (34.62%), Postives = 68/130 (52.31%), Query Frame = 3 
Query:  501 CTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASNFWLIISWILTIICVLY 890
            CT +  Y  WCRFS + AP FN+L R FP L F+AL  +          T  +P I ++ G+K +  FN +D  L+ L  FI+  TG     +  +T+ D  GP+PS L  + ++ L+ S    I  ++Y
Sbjct:  210 CTLVLFYTPWCRFSASLAPHFNSLPRAFPALHFLALDASQHSSLSTRFGTVAVPNILLFQGAKPMARFNHTDRTLETLKIFIFNQTGIEAKKNVVVTQADQIGPLPSTLIKSVDWLLVFSLFFLISFIMY 339
[Close]
BLAST of Thioredoxin domain-containing protein vs. UniProt/SwissProt
Match: sp|Q5BJT4|TXD15_RAT (Thioredoxin domain-containing protein 15 OS=Rattus norvegicus OX=10116 GN=Txndc15 PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 1.141e-16
Identity = 45/130 (34.62%), Postives = 67/130 (51.54%), Query Frame = 3 
Query:  501 CTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASNFWLIISWILTIICVLY 890
            CT +  Y  WCRFS + AP FN+L R FP L F+AL  +          T  +P I ++ G+K +  FN +D  L+ L  FI+  TG     +  +T+ D  GP+PS L    ++ L+ S    I  ++Y
Sbjct:  193 CTLVLFYTPWCRFSASLAPHFNSLPRAFPTLGFLALDASQHSSLSTRFGTVAVPNILLFQGAKPMARFNHTDRTLETLKIFIFNQTGIEAKKNVVVTQADQLGPLPSTLVKTVDWLLVFSLFFLISFIMY 322
[Close]
BLAST of Thioredoxin domain-containing protein vs. UniProt/SwissProt
Match: sp|Q6P6J9|TXD15_MOUSE (Thioredoxin domain-containing protein 15 OS=Mus musculus OX=10090 GN=Txndc15 PE=1 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 1.164e-16
Identity = 45/130 (34.62%), Postives = 67/130 (51.54%), Query Frame = 3 
Query:  501 CTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASNFWLIISWILTIICVLY 890
            CT +  Y  WCRFS + AP FN+L R FP L F+AL  +          T  +P I ++ G+K +  FN +D  L+ L  FI+  TG     +  +T+ D  GP+PS L    ++ L+ S    I  ++Y
Sbjct:  194 CTLVLFYTPWCRFSASLAPHFNSLPRAFPTLGFLALDASQHSSLSTRFGTVAVPNILLFQGAKPMARFNHTDRTLETLKIFIFNQTGIEAKKNVVVTQADQMGPLPSTLIKTVDWLLVFSLFFLISFIMY 323
[Close]
BLAST of Thioredoxin domain-containing protein vs. TrEMBL
Match: A0A0R3ULM0 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS8579 PE=4 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 7.294e-37
Identity = 77/214 (35.98%), Postives = 115/214 (53.74%), Query Frame = 3 
Query:  336 CLLNRINYWNYKSGTVKIPIVMQMDR--------PVEKE-------NSKYGISEAAN-------NSNETVKKSEPKK-----CTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASN-FWLIISWILTIICVLYL 893
            C L  +NYWNY SG   + +  Q D         P+  +          + +S A N           T ++  P K     CT +FI+A+WC FSM AAP  NAL R FPQL  +A+  N++ +++W+L   YIP+IK++ G    REFNG+D DLD L +F++++   LP+    L ++DF GPIP+QL    N   L  +W++ ++C  YL
Sbjct:   22 CRLRSVNYWNYTSGHASLVLGHQFDSAVGALYSPPISHDLLGLYVNRHPFKLSSALNPFFPSLRAGYATSRRKTPTKVTESLCTLVFIFAQWCPFSMRAAPYINALARAFPQLPIVAIESNEYLQYRWSLRVFYIPKIKIFIGGHVYREFNGTDYDLDELADFLWLSLRLLPIGPLELRREDFVGPIPTQLMIRENDLRLPAAWVIFLVCFAYL 235
[Close]
BLAST of Thioredoxin domain-containing protein vs. TrEMBL
Match: A0A5K3FC86 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 2.394e-36
Identity = 77/214 (35.98%), Postives = 115/214 (53.74%), Query Frame = 3 
Query:  336 CLLNRINYWNYKSGTVKIPIVMQMDR--------PVEKE-------NSKYGISEAAN-------NSNETVKKSEPKK-----CTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASN-FWLIISWILTIICVLYL 893
            C L  +NYWNY SG   + +  Q D         P+  +          + +S A N           T ++  P K     CT +FI+A+WC FSM AAP  NAL R FPQL  +A+  N++ +++W+L   YIP+IK++ G    REFNG+D DLD L +F++++   LP+    L ++DF GPIP+QL    N   L  +W++ ++C  YL
Sbjct:   73 CRLRSVNYWNYTSGHASLVLGHQFDSAVGALYSPPISHDLLGLYVNRHPFKLSSALNPFFPSLRAGYATSRRKTPTKVTESLCTLVFIFAQWCPFSMRAAPYINALARAFPQLPIVAIESNEYLQYRWSLRVFYIPKIKIFIGGHVYREFNGTDYDLDELADFLWLSLRLLPIGPLELRREDFVGPIPTQLMIRENDLRLPAAWVIFLVCFAYL 286
[Close]
BLAST of Thioredoxin domain-containing protein vs. TrEMBL
Match: A0A183S8T7 (Thioredoxin domain-containing protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS635 PE=4 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 8.045e-36
Identity = 72/227 (31.72%), Postives = 119/227 (52.42%), Query Frame = 3 
Query:  336 CLLNRINYWNYKSGTVKIPIVMQMDRPVEKENSKYGISEAANNSNETVKKSE----------------------PKKCTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASNFWLIISWILTIICVLYLIATTGRGRRTILAMLRCMK 950
            C L  +N+WNY   + K+ +  +++  +       G      N +  V+ S                          CT +F+YA WC FS+AAAP  NAL R FPQL  +A+ ++++ ++KW+L   YIP++K++      REFNGSD+DLD +++F++ +   LPM    L   DF GPIPS+L   ++  L ++W++ + CV+YL+      RR    ++  +K
Sbjct:   47 CRLRPVNWWNYTLASSKLILGHELESALGTALGVGGSDIFGVNLHRHVRLSSLFSPFLLQRRYPTSKSAVPHIIQSHCTLVFVYASWCPFSVAAAPYVNALARAFPQLPLVAIDVDEYVKYKWSLRVFYIPKLKIFIAGHVYREFNGSDTDLDEMIDFVWNSIRLLPMGPVRLEGRDFLGPIPSELHPCNDIRLPLAWLVFLACVIYLVGCFVDYRRLCANLIALVK 273
[Close]
BLAST of Thioredoxin domain-containing protein vs. TrEMBL
Match: A0A0R3W1F2 (Thioredoxin domain-containing protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS3561 PE=4 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 3.497e-35
Identity = 84/267 (31.46%), Postives = 139/267 (52.06%), Query Frame = 3 
Query:  249 SDINSTSNGKTLKKISGIEMNEEYKRIYPCLLNRINYWNYKSGTVKIPIVMQMDRPVEKENSK----YGI-SEAANNSNETVK---------------------KSEPK----KCTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAA-SNFWLIISWILTIICVLYLIA---TTGRGRRTILAMLRCM 947
             +++S ++    K +   E  E  K I  C L  +NYWNY  G  ++ +  +    V   +S     YGI S AA+   + ++                     KS  +     C  +FI+A+WC FSM AAP  NA+ R FPQL  IA+ ++++ +F+W+L   Y+P+IK++ G  + +EFNG+++DL+ L +F++ N  Q+P     L K+DF GPIP+QL +  S++ L I W++ ++   Y +         RR + A L+ M
Sbjct:   18 QEVSSDASSNWRKDVDNSESGE--KVITRCHLRPVNYWNYTQGRARVVLGHEFGTAVGAIHSSSAPPYGIFSGAAHPYRQNLRLFSLLNPFFSHLRGNYVFVKNKSSARLIDCPCNLVFIFAQWCPFSMRAAPYINAIARAFPQLPVIAIDVDEYLQFRWSLRVFYVPKIKIFVGDHAYKEFNGTETDLNELADFVWQNLRQIPQGPLELRKEDFYGPIPTQLLSRFSDYRLQIVWVIFLVSSAYFVGYFVDCRELRRHVTAALKLM 282
[Close]
BLAST of Thioredoxin domain-containing protein vs. TrEMBL
Match: W6URG6 (Thioredoxin domain-containing protein OS=Echinococcus granulosus OX=6210 GN=EGR_09201 PE=4 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 4.495e-35
Identity = 84/265 (31.70%), Postives = 135/265 (50.94%), Query Frame = 3 
Query:  255 INSTSNGKTLKKISGIEMNEEYKRIYPCLLNRINYWNYKSGTVKIPIVMQMDRPVEKENSK----YGISEAANN--------------------------SNETVKKSEPKKCTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEA-ASNFWLIISWILTIICVLYLIA--TTGRG-RRTILAMLRCM 947
            ++++SN +  K +   E  E  K I  C L  +NYWNY  G  K+    +    V   +S     YG+   + N                           N++  +     C  +FI+A+WC FSM AAP  NAL R FPQL  IA+ ++++ +F+W+L   Y+P+IK++ G    +EFNG+++DLD L +F++ N  Q+P     L ++DF GPIP+QL +  S++ L + W++ ++   Y        RG RR I A L+ +
Sbjct:   14 VDASSNWQ--KDVDNFESGE--KVITRCRLRSVNYWNYTQGRAKVVPGYEFGTVVGVSHSPNSPPYGVFSGSVNPYRHNLRLFSLLNPFFSHLRGNYVFVKNKSSARLIDSPCNLVFIFAQWCPFSMRAAPYINALARAFPQLPVIAIDVDEYLQFRWSLRVFYVPKIKIFVGDHVYKEFNGTETDLDELADFVWQNLRQIPQGPLELRREDFFGPIPTQLVSRPSDYRLQVVWVIFLVSSAYFFGYFVDCRGVRRHITAALKLV 274
[Close]
BLAST of Thioredoxin domain-containing protein vs. Ensembl Cavefish
Match: txndc15 (thioredoxin domain containing 15 [Source:NCBI gene;Acc:103039559])

HSP 1 Score: 86.6557 bits (213), Expect = 2.471e-18
Identity = 50/144 (34.72%), Postives = 74/144 (51.39%), Query Frame = 3 
Query:  498 KCTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASNFWLIISWILTIICV-LYLIATTGRGRRTI 926
            +CT +  +  WC+FS   AP FNAL R+FP + F+AL  +          T  +P I ++ G+K +  FN +D  LD L  FI   TG   +   ++ ++D +GP+PS +   S  WL +  IL I+   LY I  T   R  I
Sbjct:  176 ECTLVLFFTTWCQFSANLAPHFNALPRVFPSMHFLALDASQHSSLSTRFGTVAVPNILLFQGAKPMARFNHTDRTLDTLTSFITNQTGFEAVPGQAVGEEDQAGPLPS-VPVKSIDWLFVFSILFILGFSLYAILRTDSIRLLI 318
[Close]
BLAST of Thioredoxin domain-containing protein vs. Ensembl Nematostella
Match: EDO34022 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQL3])

HSP 1 Score: 60.8474 bits (146), Expect = 2.726e-10
Identity = 37/133 (27.82%), Postives = 61/133 (45.86%), Query Frame = 3 
Query:  501 CTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASNFWLIISWILTIICVLYLIA 899
            C  +  Y ++C FS   AP+FNA GR +     +AL           L     P I ++   K V +FN S + L  L  F+  +TG     S  ++ +D+ GP+ S      N++L IS +  +   L++ +
Sbjct:  144 CAVVLFYTRFCVFSAKLAPMFNAFGRAYNNTPVLALDAYQHNSMNARLGVVATPTILIFHAGKPVLKFNHSQT-LHDLRTFVKNHTGLEGNSSIEVSTEDYDGPVSSITIKEPNYYLAISSLFLLFYGLFVFS 275
[Close]
BLAST of Thioredoxin domain-containing protein vs. Ensembl Medaka
Match: txndc15 (thioredoxin domain containing 15 [Source:NCBI gene;Acc:101171512])

HSP 1 Score: 78.9518 bits (193), Expect = 1.319e-15
Identity = 43/126 (34.13%), Postives = 65/126 (51.59%), Query Frame = 3 
Query:  498 KCTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASNFWLIISWILTI 875
            +C+ +  Y  WC+FS   AP FNAL R FP + F+AL  +          T  +P I ++ G+K +  FN +D  L+ L  FI   TG       ++T++D  GP+ S +   S  WL++  IL I
Sbjct:  199 ECSVVLFYTAWCQFSANLAPHFNALPRAFPGIHFLALDASQHSSLSTRFGTVAVPNILLFQGAKPMARFNHTDRTLETLTSFITNQTGFEANPDRNVTEEDLEGPLLS-VPMRSIDWLLVFSILFI 323
[Close]
BLAST of Thioredoxin domain-containing protein vs. Planmine SMEST
Match: SMESG000063824.1 (SMESG000063824.1)

HSP 1 Score: 563.533 bits (1451), Expect = 0.000e+0
Identity = 289/295 (97.97%), Postives = 291/295 (98.64%), Query Frame = 3 
Query:   66 MNINXXXXXXXXXXXXXIKSFENDTVITIEPRFFFKELLQIWSLYFFRSKPVGPLATSSNLSDINSTSNGKTLKKISGIEMNEEYKRIYPCLLNRINYWNYKSGTVKIPIVMQMDRPVEKENSKYGISEAANNSNETVKKSEPKKCTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASNFWLIISWILTIICVLYLIATTGRGRRTILAMLRCMK 950
            MNINFLFLLVFFCYFCCIKSFENDTVITIEPRFFFKELLQIWSLYFFRSKPVGPLATSSNLSDINSTSNGKTLK  SGIEMNEEYKRIYPCLLNRINYWNYKSG VKIPIV+QMDRPVEKENSKYGISEAANNSNET+KKSEPKKCTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASNFWLIISWILTIIC LYLIATTGRGRRTILAMLRCMK
Sbjct:    1 MNINFLFLLVFFCYFCCIKSFENDTVITIEPRFFFKELLQIWSLYFFRSKPVGPLATSSNLSDINSTSNGKTLKNFSGIEMNEEYKRIYPCLLNRINYWNYKSGAVKIPIVLQMDRPVEKENSKYGISEAANNSNETIKKSEPKKCTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASNFWLIISWILTIICALYLIATTGRGRRTILAMLRCMK 295
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BLAST of Thioredoxin domain-containing protein vs. Planmine SMEST
Match: SMESG000063824.1 (SMESG000063824.1)

HSP 1 Score: 442.965 bits (1138), Expect = 4.211e-156
Identity = 211/215 (98.14%), Postives = 213/215 (99.07%), Query Frame = 3 
Query:  306 MNEEYKRIYPCLLNRINYWNYKSGTVKIPIVMQMDRPVEKENSKYGISEAANNSNETVKKSEPKKCTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASNFWLIISWILTIICVLYLIATTGRGRRTILAMLRCMK 950
            MNEEYKRIYPCLLNRINYWNYKSG VKIPIV+QMDRPVEKENSKYGISEAANNSNET+KKSEPKKCTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASNFWLIISWILTIIC LYLIATTGRGRRTILAMLRCMK
Sbjct:    1 MNEEYKRIYPCLLNRINYWNYKSGAVKIPIVLQMDRPVEKENSKYGISEAANNSNETIKKSEPKKCTFIFIYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPRIKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPIPSQLEAASNFWLIISWILTIICALYLIATTGRGRRTILAMLRCMK 215
The following BLAST results are available for this feature:
BLAST of Thioredoxin domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
TXNDC157.531e-1834.62thioredoxin domain containing 15 [Source:HGNC Symb... [more]
TXNDC151.072e-1734.62thioredoxin domain containing 15 [Source:HGNC Symb... [more]
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BLAST of Thioredoxin domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Thioredoxin domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Thioredoxin domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
txndc151.762e-1531.50thioredoxin domain containing 15 [Source:ZFIN;Acc:... [more]
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BLAST of Thioredoxin domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
TXNDC154.906e-1932.86thioredoxin domain containing 15 [Source:NCBI gene... [more]
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BLAST of Thioredoxin domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Txndc151.664e-1734.62thioredoxin domain containing 15 [Source:MGI Symbo... [more]
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BLAST of Thioredoxin domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q0IHI1|TXD15_XENLA1.775e-1832.86Thioredoxin domain-containing protein 15 OS=Xenopu... [more]
sp|Q96J42|TXD15_HUMAN5.149e-1734.62Thioredoxin domain-containing protein 15 OS=Homo s... [more]
sp|Q5BJT4|TXD15_RAT1.141e-1634.62Thioredoxin domain-containing protein 15 OS=Rattus... [more]
sp|Q6P6J9|TXD15_MOUSE1.164e-1634.62Thioredoxin domain-containing protein 15 OS=Mus mu... [more]
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BLAST of Thioredoxin domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0R3ULM07.294e-3735.98Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A5K3FC862.394e-3635.98Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A183S8T78.045e-3631.72Thioredoxin domain-containing protein OS=Schistoce... [more]
A0A0R3W1F23.497e-3531.46Thioredoxin domain-containing protein OS=Taenia as... [more]
W6URG64.495e-3531.70Thioredoxin domain-containing protein OS=Echinococ... [more]
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BLAST of Thioredoxin domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
txndc152.471e-1834.72thioredoxin domain containing 15 [Source:NCBI gene... [more]
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BLAST of Thioredoxin domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Thioredoxin domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Thioredoxin domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO340222.726e-1027.82Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Thioredoxin domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
txndc151.319e-1534.13thioredoxin domain containing 15 [Source:NCBI gene... [more]
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BLAST of Thioredoxin domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000063824.10.000e+097.97SMESG000063824.1[more]
SMESG000063824.14.211e-15698.14SMESG000063824.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015682 ID=SMED30015682|Name=Thioredoxin domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1509bp
ATTAAACATCACTTTTTACAATATTGTTCACTTTATTTGAATTTAATTTT
TTAATTTATCATTCCATGAACATTAATTTTTTATTTCTATTAGTTTTTTT
TTGTTATTTTTGTTGTATAAAATCATTTGAAAATGATACAGTCATAACTA
TTGAACCTAGGTTTTTCTTTAAAGAGCTTTTGCAAATATGGTCCTTGTAT
TTCTTTCGTTCAAAACCAGTTGGGCCTTTAGCTACTTCTAGTAATTTATC
AGATATAAATTCAACGAGTAATGGGAAAACTTTAAAAAAAATTTCAGGCA
TTGAAATGAATGAAGAATATAAACGCATTTATCCTTGTCTTTTAAATCGT
ATCAATTATTGGAATTATAAAAGTGGTACCGTGAAAATTCCAATTGTCAT
GCAAATGGATCGACCTGTTGAAAAAGAGAATTCTAAATATGGAATTAGCG
AAGCAGCTAACAATAGCAATGAAACTGTAAAAAAGTCCGAACCAAAAAAA
TGCACATTTATATTCATATATGCTAAATGGTGCAGATTCAGCATGGCAGC
GGCGCCAGTATTCAATGCGCTTGGACGCATTTTTCCGCAATTGGAGTTTA
TTGCTCTGGCAATAAATGACTTTCAAGAATTCAAATGGAATCTTCACACG
AGCTACATACCTCGAATCAAGATGTATTTCGGCAGCAAATCTGTCCGTGA
ATTCAACGGTTCCGATAGCGACCTGGATGCGTTGGTGGAGTTCATCTATG
TGAACACGGGTCAGCTGCCTATGTTGAGCTCCAGTCTCACCAAAGATGAT
TTCTCCGGGCCAATTCCCAGTCAATTGGAGGCAGCCAGCAATTTTTGGCT
CATAATTTCCTGGATTCTGACGATCATTTGCGTCTTGTATTTGATCGCCA
CAACAGGCCGAGGAAGACGCACCATTCTAGCCATGCTGCGTTGCATGAAA
TAGTGCTACGAGCTAGTGAATTTGGGCTTTGTCATTTGTTCAAAATTTAT
CTCATTTATCATTGATTTACCCGTTGTGTTATTTTGCGCACTTTTGGAAA
TGATCACAGCTTGAATAGCCCCCTTTCCACCAACCCAATGTGCTCATCTG
TTATCATATAGCGAGCATCTAATAGAGTAATTTCATTATCTGAAAATCTC
TTATTGGCTGCTGCCCGAACCAATTATTCCTACCCATGATAACACCAAGC
CTATTATTATCTGTTCAACCGCTGTTTGAGCAATTATTTAGGGCAGCCGC
TGCTACTGTGATCAACTAGTGCTATATCATTATTATATCGATGCGCAAAT
TACTTATAAGTATTTATGACTACATTTTATGACTGCACTAAATTTTGATG
TATTCTGGTGAAAATTTTGAAATGTTGCAACAATATATCTAAACCGCCAA
CAAAAAGCAGTCAAATAGGACAAAAAATAGTTAACATCAAATTATTTGCT
GTATATGACAGTTTATGCAGAATATTATTGAAAATCCCCTGATTAAAACT
CGTTTTTCA
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protein sequence of SMED30015682-orf-1

>SMED30015682-orf-1 ID=SMED30015682-orf-1|Name=SMED30015682-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=296bp
MNINFLFLLVFFCYFCCIKSFENDTVITIEPRFFFKELLQIWSLYFFRSK
PVGPLATSSNLSDINSTSNGKTLKKISGIEMNEEYKRIYPCLLNRINYWN
YKSGTVKIPIVMQMDRPVEKENSKYGISEAANNSNETVKKSEPKKCTFIF
IYAKWCRFSMAAAPVFNALGRIFPQLEFIALAINDFQEFKWNLHTSYIPR
IKMYFGSKSVREFNGSDSDLDALVEFIYVNTGQLPMLSSSLTKDDFSGPI
PSQLEAASNFWLIISWILTIICVLYLIATTGRGRRTILAMLRCMK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000499cell
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0003001lateral region of the whole animal
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: INTERPRO
TermDefinition
IPR036249Thioredoxin-like_sf
IPR042418TXNDC15
IPR013766Thioredoxin_domain
IPR012336Thioredoxin-like_fold
Vocabulary: biological process
TermDefinition
GO:0045454cell redox homeostasis
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013766Thioredoxin domainPFAMPF00085Thioredoxincoord: 132..227
e-value: 8.7E-6
score: 25.7
IPR036249Thioredoxin-like superfamilyGENE3DG3DSA:3.40.30.10coord: 118..234
e-value: 1.2E-10
score: 43.1
IPR036249Thioredoxin-like superfamilySUPERFAMILYSSF52833Thioredoxin-likecoord: 130..228
IPR042418Thioredoxin domain-containing protein 15PANTHERPTHR14684UNCHARACTERIZEDcoord: 134..280
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..20
score: 0.872
NoneNo IPR availableTMHMMTMhelixcoord: 256..278