Baculoviral IAP repeat-containing protein 7

Overview
NameBaculoviral IAP repeat-containing protein 7
Smed IDSMED30015671
Length (bp)765
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Baculoviral IAP repeat-containing protein 7 (SMED30015671) t-SNE clustered cells

Violin plots show distribution of expression levels for Baculoviral IAP repeat-containing protein 7 (SMED30015671) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Baculoviral IAP repeat-containing protein 7 (SMED30015671) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Baculoviral IAP repeat-containing protein 7 (SMED30015671) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30015671

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymal cellSMED30015671SMESG000067794.1 dd_Smed_v4_10803_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Baculoviral IAP repeat-containing protein 7 vs. TrEMBL
Match: I1ZIH9 (IAP domain containing-1 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 6.327e-12
Identity = 34/50 (68.00%), Postives = 38/50 (76.00%), Query Frame = 1
Query:  532 LTCFECKENQVSILYLPCGHLVTCGTCFVSSRKHCQVCETPIDNFHLIYL 681
            LTC  CK+NQVSILY PC H+V C TCFVSS K CQVC  PI  +HLIY+
Sbjct:    7 LTCNFCKKNQVSILYEPCYHVVICRTCFVSSMKICQVCNVPIHQYHLIYI 56          
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Medaka
Match: ENSORLT00000012200.2 (baculoviral IAP repeat containing 7 [Source:NCBI gene;Acc:101164308])

HSP 1 Score: 46.9802 bits (110), Expect = 8.166e-6
Identity = 21/45 (46.67%), Postives = 28/45 (62.22%), Query Frame = 1
Query:  523 KSKLTCFECKENQVSILYLPCGHLVTCGTCFVSSRKHCQVCETPI 657
            + + TC  C +  VSI+++PCGHLV CG C  S R HC +C   I
Sbjct:  311 QEERTCKVCMDKLVSIVFIPCGHLVVCGDCAASLR-HCPICRAVI 354          
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Medaka
Match: ENSORLT00000039931.1 (baculoviral IAP repeat containing 7 [Source:NCBI gene;Acc:101164308])

HSP 1 Score: 46.9802 bits (110), Expect = 8.166e-6
Identity = 21/45 (46.67%), Postives = 28/45 (62.22%), Query Frame = 1
Query:  523 KSKLTCFECKENQVSILYLPCGHLVTCGTCFVSSRKHCQVCETPI 657
            + + TC  C +  VSI+++PCGHLV CG C  S R HC +C   I
Sbjct:  311 QEERTCKVCMDKLVSIVFIPCGHLVVCGDCAASLR-HCPICRAVI 354          
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Planmine SMEST
Match: SMESG000067794.1 (SMESG000067794.1)

HSP 1 Score: 360.533 bits (924), Expect = 1.215e-127
Identity = 186/189 (98.41%), Postives = 187/189 (98.94%), Query Frame = 1
Query:  115 FHNNAKVICDPHCESVYENEIPESLFRFFKINFYDLVSNPQNIEPFQLAQISESLRHPSIRFLRQTFDFNESILKMCTCLLQLNLMEIDNEILNDYFWKFSTLRLTDPDSRNIIQYWLENXXXXXXXXXXRLKAFSKSKLTCFECKENQVSILYLPCGHLVTCGTCFVSSRKHCQVCETPIDNFHLIYL 681
            FHNNAKVICDPHCESVYENEIPESLF+FFKINFYDLVSNPQNIEPFQLAQISESLRHPSIRFLRQTFDFNESILKMCTCLLQLNLMEIDNEILNDYFWKFSTLRLTDPDSR IIQYWLENFEKIEKFIEERLK FSKSKLTCFECKENQVSILYLPCGHLVTCGTCFVSSRKHCQVCETPIDNFHLIYL
Sbjct:   14 FHNNAKVICDPHCESVYENEIPESLFQFFKINFYDLVSNPQNIEPFQLAQISESLRHPSIRFLRQTFDFNESILKMCTCLLQLNLMEIDNEILNDYFWKFSTLRLTDPDSRTIIQYWLENFEKIEKFIEERLKEFSKSKLTCFECKENQVSILYLPCGHLVTCGTCFVSSRKHCQVCETPIDNFHLIYL 202          
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Planmine SMEST
Match: SMESG000067794.1 (SMESG000067794.1)

HSP 1 Score: 355.525 bits (911), Expect = 9.445e-126
Identity = 186/190 (97.89%), Postives = 187/190 (98.42%), Query Frame = 1
Query:  115 FHNNAKVICDPHCESV-YENEIPESLFRFFKINFYDLVSNPQNIEPFQLAQISESLRHPSIRFLRQTFDFNESILKMCTCLLQLNLMEIDNEILNDYFWKFSTLRLTDPDSRNIIQYWLENXXXXXXXXXXRLKAFSKSKLTCFECKENQVSILYLPCGHLVTCGTCFVSSRKHCQVCETPIDNFHLIYL 681
            FHNNAKVICDPHCESV YENEIPESLF+FFKINFYDLVSNPQNIEPFQLAQISESLRHPSIRFLRQTFDFNESILKMCTCLLQLNLMEIDNEILNDYFWKFSTLRLTDPDSR IIQYWLENFEKIEKFIEERLK FSKSKLTCFECKENQVSILYLPCGHLVTCGTCFVSSRKHCQVCETPIDNFHLIYL
Sbjct:   14 FHNNAKVICDPHCESVVYENEIPESLFQFFKINFYDLVSNPQNIEPFQLAQISESLRHPSIRFLRQTFDFNESILKMCTCLLQLNLMEIDNEILNDYFWKFSTLRLTDPDSRTIIQYWLENFEKIEKFIEERLKEFSKSKLTCFECKENQVSILYLPCGHLVTCGTCFVSSRKHCQVCETPIDNFHLIYL 203          
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Planmine SMEST
Match: SMESG000067789.1 (SMESG000067789.1)

HSP 1 Score: 113.235 bits (282), Expect = 1.948e-30
Identity = 60/176 (34.09%), Postives = 99/176 (56.25%), Query Frame = 1
Query:  154 ESVYENEIPESLFRFFKINFYDLVSNPQNIEPFQLAQISESLRHPSIRFLRQTFDFNESILKMCTCLLQLNLMEIDNEILNDYFWKFSTLRLTDPDSRNIIQYWLENXXXXXXXXXXRLKAFSKSKLTCFECKENQVSILYLPCGHLVTCGTCFVSSRKHCQVCETPIDNFHLIYL 681
            +++   + P   F  FK    D ++        ++  I +  ++PSI +L   F++++ ILK+ + L+ L+   +DN+ LN+YFW    LRL +P+   + + W+   + IE  + E+L    K  LTC  CKENQVS+LYLPCGH++ C  C   + + CQ C  PI+ +H IY+
Sbjct:   37 KTIISEQKPSMEFIEFKSTVEDFINTYDTFTDKEIINILKIFKNPSIEYLIDIFNYDKCILKLSSYLMYLDKTGMDNDSLNEYFWDCMELRL-NPEYSRLFKEWIRKSDFIEDKLTEKLNDVKKRYLTCSICKENQVSVLYLPCGHVIVCSKCQDKNNRKCQACSAPINEYHAIYV 211          
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Planmine SMEST
Match: SMESG000067788.1 (SMESG000067788.1)

HSP 1 Score: 111.694 bits (278), Expect = 7.012e-30
Identity = 58/155 (37.42%), Postives = 88/155 (56.77%), Query Frame = 1
Query:  217 DLVSNPQNIEPFQLAQISESLRHPSIRFLRQTFDFNESILKMCTCLLQLNLMEIDNEILNDYFWKFSTLRLTDPDSRNIIQYWLENXXXXXXXXXXRLKAFSKSKLTCFECKENQVSILYLPCGHLVTCGTCFVSSRKHCQVCETPIDNFHLIYL 681
            +L  N   +    L +I +  ++ SI+F  +TF +N+ ILK+C+ L++ N + I++E LNDYFW F  L   D   + + +                +K  +K  LTC  C++N VSILYLPCGH+V C  C+  S KHCQ C + I+ FH+IY+
Sbjct:   71 ELFRNYAKLNSKILLEILKLFKNSSIKFCHETFGYNKKILKLCSFLMERNKVNINHENLNDYFWSFMNLSQLDGIEKEMCEI---------------VKERNKQLLTCMGCQKNSVSILYLPCGHVVMCNKCYQISTKHCQACGSSIEEFHVIYI 210          
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Planmine SMEST
Match: SMESG000031926.1 (SMESG000031926.1)

HSP 1 Score: 99.7525 bits (247), Expect = 1.479e-25
Identity = 52/153 (33.99%), Postives = 92/153 (60.13%), Query Frame = 1
Query:  223 VSNPQNIEPFQLAQISESLRHPSIRFLRQTFDFNESILKMCTCLLQLNLMEIDNEILNDYFWKFSTLRLTDPDSRNIIQYWLENXXXXXXXXXXRLKAFSKSKLTCFECKENQVSILYLPCGHLVTCGTCFVSSRKHCQVCETPIDNFHLIYL 681
            +S+    +   L ++++S  H SI  L + F +N ++LK+ +C++ LN ++  +   N  FW     R+     + +IQY++ N + +++ I ++LK  ++  LTC  C+ NQVS+LYLPCGH+V C +CF S+ + C+ C   ID +H +YL
Sbjct:   48 LSSTNGFDLIFLNKLNKSFNHESIENLSKLFGYNHAMLKLSSCVISLNDIKPTDFNTNLVFWSCMEFRV--GQYQKLIQYFMANVDNLQQNINDKLKEATRLFLTCSCCETNQVSVLYLPCGHIVVCSSCFKSTTRRCESCAVDIDEYHFVYL 198          
The following BLAST results are available for this feature:
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
I1ZIH96.327e-1268.00IAP domain containing-1 (Fragment) OS=Schmidtea me... [more]
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSORLT00000012200.28.166e-646.67baculoviral IAP repeat containing 7 [Source:NCBI g... [more]
ENSORLT00000039931.18.166e-646.67baculoviral IAP repeat containing 7 [Source:NCBI g... [more]
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BLAST of Baculoviral IAP repeat-containing protein 7 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000067794.11.215e-12798.41SMESG000067794.1[more]
SMESG000067794.19.445e-12697.89SMESG000067794.1[more]
SMESG000067789.11.948e-3034.09SMESG000067789.1[more]
SMESG000067788.17.012e-3037.42SMESG000067788.1[more]
SMESG000031926.11.479e-2533.99SMESG000031926.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015671 ID=SMED30015671|Name=Baculoviral IAP repeat-containing protein 7|organism=Schmidtea mediterranea sexual|type=transcript|length=765bp
CAATTTTGGCTGTACAGAAAATAATGTCTCTACTAAATTTGTGAATAATT
TAACAATTTTATTGATTAAATCTATATGGATAAAATCAAAAAAGCTGATA
TCGATAAAAAAAAGTTTCATAATAATGCCAAGGTCATTTGTGATCCTCAT
TGTGAATCAGTCTATGAGAACGAAATTCCGGAAAGTCTGTTTCGATTTTT
CAAAATCAATTTCTATGATTTGGTTTCAAATCCGCAAAACATTGAACCAT
TTCAATTGGCTCAAATCTCCGAATCATTGAGGCACCCGTCAATAAGATTT
CTCCGGCAAACTTTCGATTTCAATGAATCGATATTGAAAATGTGCACCTG
CCTGTTACAATTGAATTTAATGGAAATCGATAATGAAATTTTGAATGATT
ACTTTTGGAAATTTAGCACTCTAAGACTTACGGATCCGGATTCCAGGAAT
ATTATTCAATATTGGCTAGAGAATTTTGAGAAGATAGAAAAATTTATTGA
GGAAAGATTAAAAGCATTTAGTAAAAGTAAATTGACCTGTTTTGAGTGCA
AGGAAAATCAAGTTTCGATTCTTTATTTGCCCTGCGGTCATTTGGTGACC
TGTGGCACTTGTTTTGTCAGTAGTCGGAAACACTGCCAGGTCTGTGAGAC
CCCCATAGATAACTTTCATTTGATTTATTTGTAATGATTTCCCATTTTAT
TTTCACACTTATTTTACTTTGACTTATTCATTTTATTATTTTTTTTGATT
TTCGGATGATATTTC
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protein sequence of SMED30015671-orf-1

>SMED30015671-orf-1 ID=SMED30015671-orf-1|Name=SMED30015671-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=203bp
MDKIKKADIDKKKFHNNAKVICDPHCESVYENEIPESLFRFFKINFYDLV
SNPQNIEPFQLAQISESLRHPSIRFLRQTFDFNESILKMCTCLLQLNLME
IDNEILNDYFWKFSTLRLTDPDSRNIIQYWLENFEKIEKFIEERLKAFSK
SKLTCFECKENQVSILYLPCGHLVTCGTCFVSSRKHCQVCETPIDNFHLI
YL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0004869cysteine-type endopeptidase inhibitor activity
GO:0030414peptidase inhibitor activity
GO:0043027cysteine-type endopeptidase inhibitor activity involved in apoptotic process
GO:0046872metal ion binding
GO:0004842ubiquitin-protein transferase activity
GO:0005515protein binding
GO:0008270zinc ion binding
GO:0016740transferase activity
Vocabulary: biological process
TermDefinition
GO:0007275multicellular organism development
GO:0010466negative regulation of peptidase activity
GO:0043154negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0051865protein autoubiquitination
GO:0034121regulation of toll-like receptor signaling pathway
GO:0035666TRIF-dependent toll-like receptor signaling pathway
GO:0038061NIK/NF-kappaB signaling
GO:0039535regulation of RIG-I signaling pathway
GO:0042981regulation of apoptotic process
GO:0043066negative regulation of apoptotic process
GO:0043123positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:0045088regulation of innate immune response
GO:0050727regulation of inflammatory response
GO:0051291protein heterooligomerization
GO:0060544regulation of necroptotic process
GO:0060546negative regulation of necroptotic process
GO:0070424regulation of nucleotide-binding oligomerization domain containing signaling pathway
GO:1990001inhibition of cysteine-type endopeptidase activity involved in apoptotic process
GO:2000116regulation of cysteine-type endopeptidase activity
GO:0006915apoptotic process
GO:0007166cell surface receptor signaling pathway
GO:0007249I-kappaB kinase/NF-kappaB signaling
GO:0007283spermatogenesis
GO:0010803regulation of tumor necrosis factor-mediated signaling pathway
GO:0016567protein ubiquitination
GO:0016579protein deubiquitination
GO:0031398positive regulation of protein ubiquitination
GO:0033209tumor necrosis factor-mediated signaling pathway
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR017066E3ligase_BOI-like
IPR013083Znf_RING/FYVE/PHD
Vocabulary: cellular component
TermDefinition
GO:0043234protein complex
GO:0045121membrane raft
GO:0005634nucleus
GO:0005654nucleoplasm
GO:0005737cytoplasm
GO:0005829cytosol
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 132..202
e-value: 1.6E-11
score: 45.5
IPR017066E3 ubiquitin-protein ligase BOI-likePIRSFPIRSF036836SBP1_RNase_bindcoord: 133..196
e-value: 3.1E-7
score: 28.1
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 152..196
e-value: 4.5E-10
score: 39.2
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 150..200