Expressed conserved protein

Overview
NameExpressed conserved protein
Smed IDSMED30015569
Length (bp)898
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Expressed conserved protein (SMED30015569) t-SNE clustered cells

Violin plots show distribution of expression levels for Expressed conserved protein (SMED30015569) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30015569

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
testis primordiumSMED30015569SMESG000036798.1 Contig6239_SE3GPL10652PMID:20844018
Wang et al., 2010
whole organism juvenile cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Expressed conserved protein vs. TrEMBL
Match: A0A068YF63 (Expressed conserved protein OS=Echinococcus multilocularis OX=6211 GN=EmuJ_001095400 PE=4 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 1.297e-63
Identity = 101/203 (49.75%), Postives = 137/203 (67.49%), Query Frame = 2
Query:  131 PCIVIFKLCDRDFRSFRNGIMRSRNIMEKSFRGQYVGISNDVERVPPDEGFPMMNFHLGAIYFTDVKDAKSFLVQDPVFSEQFFVNSSNAFIVPLRIPVNFGYNLTALLITETVTRENFAALPPVSDTFGMEICGAVPNIADAINLERVRGIEI-PAGIRISQFPSKKVFYDWCKSEYAARFRDCMCQLRSFNTYIGTFACDV 736
            PC+V+ ++  +D  + RN I ++R+++EK +RG Y+GISND ERVPP E  P++NF +G  YF+D  DA+ F+  D  F E  F+ +S+  I+P+  P+  G   TALL TE V  EN+  +P  + TF ME CGAVPNI D I +  +R  E  P G RISQFP+KKVFYDW KS+  ARFR+ MC++RS NTYI TFA D+
Sbjct:    2 PCLVLIRVRIQDLYTLRNAIYKARSVLEKGYRGLYIGISNDAERVPPCESQPLLNFGMGLFYFSDEADARRFIYSDQGFMETDFLENSDIMIIPMLKPLLIGCYGTALLFTELVRSENYCQMPASAITFDMEKCGAVPNIVDTIQVGMIRAYEAKPGGFRISQFPNKKVFYDWVKSDCGARFREDMCKVRSMNTYIATFARDL 204          
BLAST of Expressed conserved protein vs. TrEMBL
Match: U6JGK8 (Expressed conserved protein OS=Echinococcus granulosus OX=6210 GN=EGR_05945 PE=4 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 1.297e-63
Identity = 101/203 (49.75%), Postives = 137/203 (67.49%), Query Frame = 2
Query:  131 PCIVIFKLCDRDFRSFRNGIMRSRNIMEKSFRGQYVGISNDVERVPPDEGFPMMNFHLGAIYFTDVKDAKSFLVQDPVFSEQFFVNSSNAFIVPLRIPVNFGYNLTALLITETVTRENFAALPPVSDTFGMEICGAVPNIADAINLERVRGIEI-PAGIRISQFPSKKVFYDWCKSEYAARFRDCMCQLRSFNTYIGTFACDV 736
            PC+V+ ++  +D  + RN I ++R+++EK +RG Y+GISND ERVPP E  P++NF +G  YF+D  DA+ F+  D  F E  F+ +S+  I+P+  P+  G   TALL TE V  EN+  +P  + TF ME CGAVPNI D I +  +R  E  P G RISQFP+KKVFYDW KS+  ARFR+ MC++RS NTYI TFA D+
Sbjct:    2 PCLVLIRVRIQDLYTLRNAIYKARSVLEKGYRGLYIGISNDAERVPPCESQPLLNFGMGLFYFSDEADARRFIYSDQGFMETDFLENSDIMIIPMLKPLLIGCYGTALLFTELVRSENYCQMPASAITFDMEKCGAVPNIVDTIQVGMIRAYEAKPGGFRISQFPNKKVFYDWVKSDCGARFREDMCKVRSMNTYIATFARDL 204          
BLAST of Expressed conserved protein vs. TrEMBL
Match: A0A0R3VU87 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS863 PE=4 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 1.629e-63
Identity = 100/203 (49.26%), Postives = 138/203 (67.98%), Query Frame = 2
Query:  131 PCIVIFKLCDRDFRSFRNGIMRSRNIMEKSFRGQYVGISNDVERVPPDEGFPMMNFHLGAIYFTDVKDAKSFLVQDPVFSEQFFVNSSNAFIVPLRIPVNFGYNLTALLITETVTRENFAALPPVSDTFGMEICGAVPNIADAINLERVRGIEI-PAGIRISQFPSKKVFYDWCKSEYAARFRDCMCQLRSFNTYIGTFACDV 736
            PC+V+ ++  +D  + RN I ++R+++EK +RG Y+GISND ERVPP E  P++N+ +G  YF++  DA+ F+  D  F E  F+ +S+  I+P+  P+  G   TALLITE V  EN+  +P  + TF ME CGAVPNI D I +  +R  E  P G RISQFP+KKVFYDW KS+  ARFR+ MC++RS NTYI TFA D+
Sbjct:    2 PCLVLIRVRIQDLYTLRNAIYKARSVLEKGYRGLYIGISNDAERVPPCESQPLLNYGMGLFYFSEEADARRFIYSDQGFMETDFLENSDIMIIPMLKPLLIGCYGTALLITELVRSENYCQMPASAITFDMEKCGAVPNIVDTIQVGMIRAYEAKPGGFRISQFPNKKVFYDWVKSDCGARFREDMCKVRSMNTYIATFARDL 204          
BLAST of Expressed conserved protein vs. TrEMBL
Match: A0A0X3PQQ5 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=TR1083 PE=4 SV=1)

HSP 1 Score: 205.297 bits (521), Expect = 1.619e-61
Identity = 99/203 (48.77%), Postives = 132/203 (65.02%), Query Frame = 2
Query:  131 PCIVIFKLCDRDFRSFRNGIMRSRNIMEKSFRGQYVGISNDVERVPPDEGFPMMNFHLGAIYFTDVKDAKSFLVQDPVFSEQFFVNSSNAFIVPLRIPVNFGYNLTALLITETVTRENFAALPPVSDTFGMEICGAVPNIADAINLERVRGIEI-PAGIRISQFPSKKVFYDWCKSEYAARFRDCMCQLRSFNTYIGTFACDV 736
            P +++ ++  +D  + RN I +S +++EK + G YVGISND ERVPP E  P++NF +G  YFT+  DA+ F+  D  F E  F+  S+  I+P+  P++   N TALL TE V  EN+  +PP   TF ME CGAVPNI D I +  VR  E  P G RISQFP+KK+FYDW KS+  A FRD MC++RS NTYI TF  D+
Sbjct:   52 PALILIRVRIQDLYTLRNAIYKSLSVLEKGYSGTYVGISNDAERVPPCESQPLLNFGMGLFYFTNEADARRFIYSDQGFLESDFLECSDMMIIPMAKPMHLNSNGTALLFTEMVRSENYCQMPPSGCTFNMEQCGAVPNIVDTIQISMVRAYEARPGGYRISQFPNKKIFYDWVKSDCGAMFRDEMCKVRSLNTYIATFTKDL 254          
BLAST of Expressed conserved protein vs. TrEMBL
Match: A0A0R3UMK9 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS8989 PE=4 SV=1)

HSP 1 Score: 202.216 bits (513), Expect = 1.222e-60
Identity = 105/228 (46.05%), Postives = 138/228 (60.53%), Query Frame = 2
Query:  131 PCIVIFKLCDRDFRSFRNGIMRSRNIMEKSFRGQYVGISNDVERVPPDEGFPMMNFHLGAIYFTDVKDAKSFLVQDPVFSEQFFVNSSNAFIVPLRIPVNFGYNLTALLITETVTRENFAALPPVSDTFGMEICGAVPNIADAINLERVRGIEI-PAGIRISQFPSKKVFYDWCKS-------------------------EYAARFRDCMCQLRSFNTYIGTFACDV 736
            PC+V+ ++  +D  + RN I ++R+++EK ++G Y+GISND ERVPP E  P++N  LG  YF+D  DAK F+  D  F E  F+ +S+  I+PL  P+  G   TALL TE +  EN+  LPP S TF ME CGAVPNIAD I++  VR  E  P G RISQFP+KK+FYDW KS                         E  ARFR+ M ++RS NTYI TFA D+
Sbjct:    2 PCLVLIRVRIQDLYTLRNAIYKARSVLEKGYKGLYIGISNDAERVPPCESQPLLNLGLGLFYFSDEADAKRFIYSDQGFMETDFLENSDIMIIPLLKPLQIGAFGTALLFTELIRSENYVQLPPSSITFDMEKCGAVPNIADTIHINMVRAYEAKPGGFRISQFPNKKIFYDWVKSGELCSLASLTPLALALLALLWLSLLECGARFREDMSKVRSMNTYIATFARDL 229          
BLAST of Expressed conserved protein vs. Planmine SMEST
Match: SMESG000036798.1 (SMESG000036798.1)

HSP 1 Score: 422.165 bits (1084), Expect = 2.994e-151
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 2
Query:  122 MAPPCIVIFKLCDRDFRSFRNGIMRSRNIMEKSFRGQYVGISNDVERVPPDEGFPMMNFHLGAIYFTDVKDAKSFLVQDPVFSEQFFVNSSNAFIVPLRIPVNFGYNLTALLITETVTRENFAALPPVSDTFGMEICGAVPNIADAINLERVRGIEIPAGIRISQFPSKKVFYDWCKSEYAARFRDCMCQLRSFNTYIGTFACDV 736
            MAPPCIVIFKLCDRDFRSFRNGIMRSRNIMEKSFRGQYVGISNDVERVPPDEGFPMMNFHLGAIYFTDVKDAKSFLVQDPVFSEQFFVNSSNAFIVPLRIPVNFGYNLTALLITETVTRENFAALPPVSDTFGMEICGAVPNIADAINLERVRGIEIPAGIRISQFPSKKVFYDWCKSEYAARFRDCMCQLRSFNTYIGTFACDV
Sbjct:    1 MAPPCIVIFKLCDRDFRSFRNGIMRSRNIMEKSFRGQYVGISNDVERVPPDEGFPMMNFHLGAIYFTDVKDAKSFLVQDPVFSEQFFVNSSNAFIVPLRIPVNFGYNLTALLITETVTRENFAALPPVSDTFGMEICGAVPNIADAINLERVRGIEIPAGIRISQFPSKKVFYDWCKSEYAARFRDCMCQLRSFNTYIGTFACDV 205          
BLAST of Expressed conserved protein vs. Planmine SMEST
Match: SMESG000004187.1 (SMESG000004187.1)

HSP 1 Score: 55.4546 bits (132), Expect = 6.955e-9
Identity = 42/166 (25.30%), Postives = 75/166 (45.18%), Query Frame = 2
Query:  170 RSFRNGIMRSRNIMEKSFRGQYVGISNDVERVPPDEGFPMMNFHLGAIYFTDVKDAKSFLVQDPVFSEQFFVNSSNAFIVP-LRIPVNFGYNLTALLITETVTRENFAA--LPPVSDTFGMEICGAVPNIADAINLERVRGIEIPAGIRISQFPSKKVFYDWCKSE 658
            +SF N +++ +NI+     GQ VG S +++ +  D  +P   + +G + F  +KDAK +   DP+  +Q +++    +++P L+      Y +  L   + +  E F    LP          C       D +  ER RG      + IS +PS   + +W  SE
Sbjct:   34 KSFCNTMVKEKNIIRNYGNGQMVGFSEEIKVIEGD--WPQ-KYTIGLLRFDTMKDAKKWNFSDPLTKQQDWLDQVEIYVMPLLKAFPKKEYQVMELFNCDLINEEQFYHKFLPSYKSAIDGNGCENFIACTDKV--ERWRGRHNFCHVMISLWPSLDDYMNWRNSE 194          
BLAST of Expressed conserved protein vs. Planmine SMEST
Match: SMESG000047126.1 (SMESG000047126.1)

HSP 1 Score: 55.0694 bits (131), Expect = 9.784e-9
Identity = 41/166 (24.70%), Postives = 75/166 (45.18%), Query Frame = 2
Query:  170 RSFRNGIMRSRNIMEKSFRGQYVGISNDVERVPPDEGFPMMNFHLGAIYFTDVKDAKSFLVQDPVFSEQFFVNSSNAFIVP-LRIPVNFGYNLTALLITETVTRENFAA--LPPVSDTFGMEICGAVPNIADAINLERVRGIEIPAGIRISQFPSKKVFYDWCKSE 658
            +SF N +++ +NI+     GQ +G S +++ +  D  +P   + +G + F  +KDAK +   DP+  +Q +++    +++P L+      Y +  L   + +  E F    LP          C       D +  ER RG      + IS +PS   + +W  SE
Sbjct:   34 KSFCNTMVKEKNIIRNYGNGQMIGFSEEIKVIEGD--WPQ-KYTIGLLRFDTMKDAKKWNFSDPLTKQQDWLDQVEIYVMPLLKAFPKKEYQVMELFNCDLINEEQFYHKFLPSYKSAIDGNGCENFIACTDKV--ERWRGRHNFCHVMISLWPSLDDYMNWRNSE 194          
BLAST of Expressed conserved protein vs. Planmine SMEST
Match: SMESG000047126.1 (SMESG000047126.1)

HSP 1 Score: 53.5286 bits (127), Expect = 2.389e-8
Identity = 39/162 (24.07%), Postives = 73/162 (45.06%), Query Frame = 2
Query:  170 RSFRNGIMRSRNIMEKSFRGQYVGISNDVERVPPDEGFPMMNFHLGAIYFTDVKDAKSFLVQDPVFSEQFFVNSSNAFIVP-LRIPVNFGYNLTALLITETVTRENFAA--LPPVSDTFGMEICGAVPNIADAINLERVRGIEIPAGIRISQFPSKKVFYDW 646
            +SF N +++ +NI+     GQ +G S +++ +  D  +P   + +G + F  +KDAK +   DP+  +Q +++    +++P L+      Y +  L   + +  E F    LP          C       D +  ER RG      + IS +PS   + +W
Sbjct:   34 KSFCNTMVKEKNIIRNYGNGQMIGFSEEIKVIEGD--WPQ-KYTIGLLRFDTMKDAKKWNFSDPLTKQQDWLDQVEIYVMPLLKAFPKKEYQVMELFNCDLINEEQFYHKFLPSYKSAIDGNGCENFIACTDKV--ERWRGRHNFCHVMISLWPSLDDYMNW 190          
The following BLAST results are available for this feature:
BLAST of Expressed conserved protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Expressed conserved protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Expressed conserved protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Expressed conserved protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Expressed conserved protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Expressed conserved protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Expressed conserved protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Expressed conserved protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A068YF631.297e-6349.75Expressed conserved protein OS=Echinococcus multil... [more]
U6JGK81.297e-6349.75Expressed conserved protein OS=Echinococcus granul... [more]
A0A0R3VU871.629e-6349.26Uncharacterized protein OS=Taenia asiatica OX=6051... [more]
A0A0X3PQQ51.619e-6148.77Uncharacterized protein OS=Schistocephalus solidus... [more]
A0A0R3UMK91.222e-6046.05Uncharacterized protein OS=Mesocestoides corti OX=... [more]
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BLAST of Expressed conserved protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Expressed conserved protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Expressed conserved protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Expressed conserved protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Expressed conserved protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Expressed conserved protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000036798.12.994e-151100.00SMESG000036798.1[more]
SMESG000004187.16.955e-925.30SMESG000004187.1[more]
SMESG000047126.19.784e-924.70SMESG000047126.1[more]
SMESG000047126.12.389e-824.07SMESG000047126.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015569 ID=SMED30015569|Name=Expressed conserved protein|organism=Schmidtea mediterranea sexual|type=transcript|length=898bp
AGATTTAAATTTGAGCATCAAATTTTAGAAAATCAATAAATTCAAATTCA
ATTTAGTTATTTCCACGACTACATTAATTGTCAGCAAATTTATTTCGGAA
AAAATAAGGAAACATCAATAAATGGCTCCACCGTGCATCGTAATTTTTAA
GCTCTGTGATCGAGACTTCCGTAGCTTTAGAAATGGGATAATGCGCTCAA
GAAATATTATGGAAAAATCTTTCAGAGGACAATATGTAGGAATTTCAAAT
GACGTTGAAAGGGTACCTCCAGATGAAGGCTTTCCAATGATGAATTTTCA
CTTAGGAGCTATTTATTTTACTGATGTTAAAGATGCCAAAAGCTTTTTAG
TTCAAGATCCAGTCTTCAGTGAACAGTTTTTCGTTAACTCATCTAACGCC
TTCATAGTTCCATTAAGAATTCCAGTCAATTTTGGATACAATTTAACAGC
TTTGCTAATCACTGAAACTGTGACAAGAGAAAATTTCGCGGCTTTGCCCC
CAGTTTCTGATACTTTTGGTATGGAAATATGCGGTGCAGTGCCAAATATT
GCCGATGCAATTAATCTTGAACGAGTTCGTGGAATAGAAATTCCGGCTGG
AATACGTATTTCTCAATTTCCTTCAAAAAAAGTATTTTATGATTGGTGCA
AATCAGAATACGCTGCAAGATTTCGAGATTGCATGTGTCAGTTAAGAAGC
TTTAACACTTATATTGGCACATTTGCCTGTGATGTTTAAACACCGAAGCA
AACAAACAATATCGAGATGATTTCTTTTTTAAAAATTCCTATTTTTTCTT
TTTTTTATTTTAAATTATTTCGAAATAGAATCGTCAATTTTTTATTGAAC
ATTTTATTTCTATGAACACAAGTCACAATTTGCTTGGATTAGTGTTGC
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protein sequence of SMED30015569-orf-1

>SMED30015569-orf-1 ID=SMED30015569-orf-1|Name=SMED30015569-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=206bp
MAPPCIVIFKLCDRDFRSFRNGIMRSRNIMEKSFRGQYVGISNDVERVPP
DEGFPMMNFHLGAIYFTDVKDAKSFLVQDPVFSEQFFVNSSNAFIVPLRI
PVNFGYNLTALLITETVTRENFAALPPVSDTFGMEICGAVPNIADAINLE
RVRGIEIPAGIRISQFPSKKVFYDWCKSEYAARFRDCMCQLRSFNTYIGT
FACDV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003304testis primordium