Meiosis expressed protein 1

Overview


Name	Meiosis expressed protein 1
Smed ID	SMED30015563
Length (bp)	400

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Meiosis expressed protein 1 (SMED30015563) t-SNE clustered cells

Violin plots show distribution of expression levels for Meiosis expressed protein 1 (SMED30015563) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Meiosis expressed protein 1 (SMED30015563) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Meiosis expressed protein 1 (SMED30015563) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30015563

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 11

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
head region	SMED30015563	SMESG000031493.1	SmedASXL_001030	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
pharynx	SMED30015563	SMESG000031493.1	dd_Smed_v4_4620_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
protonephridia	SMED30015563	SMESG000031493.1	dd_Smed_v4_4620_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30015563	SMESG000031493.1	dd_Smed_v4_4620_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30015563	SMESG000031493.1	dd_Smed_v4_4620_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30015563	SMESG000031493.1	dd_Smed_v4_4620_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neuron	SMED30015563	SMESG000031493.1	dd_Smed_v4_4620_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cilated neuron	SMED30015563	SMESG000031493.1	dd_Smed_v4_4620_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30015563	SMESG000031493.1	Contig41618	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30015563	SMESG000031493.1	Contig41618	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
head region	SMED30015563	SMESG000031493.1	dd_Smed_v6_4620_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017

Note: Hover over icons to view figure legend

Homology

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BLAST of Meiosis expressed protein 1 vs. Ensembl Human
Match: MEIG1 (meiosis/spermiogenesis associated 1 [Source:HGNC Symbol;Acc:HGNC:23429])

HSP 1 Score: 90.8929 bits (224), Expect = 2.214e-24
Identity = 39/84 (46.43%), Postives = 61/84 (72.62%), Query Frame = 1

Query:   67 DPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRG-HVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
            D KP ++  AK W+ ++EN YRFQ  GYRDE EY+ ++   +V++WP++G+VKK+ R+D  F Y++K REC +K++ K K+Y Y
Sbjct:    5 DVKPKSVSHAKKWSEEIENLYRFQQAGYRDETEYRQVKQVSMVDRWPETGYVKKLQRRDNTFYYYNKQRECDDKEVHKVKIYAY 88

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Query:   67 DPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRG-HVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
            D KP ++  AK W+ ++EN YRFQ  GYRDE EY+ ++   +V++WP++G+VKK+ R+D  F Y++K REC +K++ K K+Y Y
Sbjct:    5 DVKPKSVSHAKKWSEEIENLYRFQQAGYRDETEYRQVKQVSMVDRWPETGYVKKLQRRDNTFYYYNKQRECDDKEVHKVKIYAY 88

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BLAST of Meiosis expressed protein 1 vs. Ensembl Zebrafish
Match: meig1 (meiosis/spermiogenesis associated 1 [Source:NCBI gene;Acc:541343])

HSP 1 Score: 98.2117 bits (243), Expect = 2.036e-27
Identity = 41/83 (49.40%), Postives = 61/83 (73.49%), Query Frame = 1

Query:   67 DPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRGHVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
            + KP +M RAK W  +VEN YRFQ  GYRDE+EY+ I+   +++WPD+GFVKK+ R+D  F Y+++ REC ++++ K K+Y Y
Sbjct:   10 NAKPKSMSRAKQWTGEVENLYRFQQAGYRDELEYRQIKQAEIDRWPDTGFVKKLQRRDNTFYYYNRKRECEDREVHKVKVYAY 92

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Query:   67 DPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRGHVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
            + KP +M RAK W  +VEN YRFQ  GYRDE+EY+ I+   +++WPD+GFVKK+ R+D  F Y+++ REC ++++ K K+Y Y
Sbjct:   10 NAKPKSMSRAKQWTGEVENLYRFQQAGYRDELEYRQIKQAEIDRWPDTGFVKKLQRRDNTFYYYNRKRECEDREVHKVKVYAY 92

[Close]

Query:   67 DPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRGHVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
            + KP +M RAK W  +VEN YRFQ  GYRDE+EY+ I+   +++WPD+GFVKK+ R+D  F Y+++ REC ++++ K K+Y Y
Sbjct:   10 NAKPKSMSRAKQWTGEVENLYRFQQAGYRDELEYRQIKQAEIDRWPDTGFVKKLQRRDNTFYYYNRKRECEDREVHKVKVYAY 92

[Close]

Query:   67 DPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRGHVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
            + KP +M RAK W  +VEN YRFQ  GYRDE+EY+ I+   +++WPD+GFVKK+ R+D  F Y+++ REC ++++ K K+Y Y
Sbjct:   10 NAKPKSMSRAKQWTGEVENLYRFQQAGYRDELEYRQIKQAEIDRWPDTGFVKKLQRRDNTFYYYNRKRECEDREVHKVKVYAY 92

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BLAST of Meiosis expressed protein 1 vs. Ensembl Xenopus
Match: b3gnt4 (UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 4 [Source:Xenbase;Acc:XB-GENE-6039654])

HSP 1 Score: 100.908 bits (250), Expect = 1.849e-28
Identity = 44/84 (52.38%), Postives = 63/84 (75.00%), Query Frame = 1

Query:   67 DPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRG-HVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
            + KP +M RAK W+ ++EN YRFQ  GYRDEVEY+ ++   +V++WP++GFVKK+ RKD  F Y++K REC EK++ K K+Y Y
Sbjct:    5 EAKPKSMTRAKQWSDEIENLYRFQTAGYRDEVEYRHVKQVEMVDRWPETGFVKKLQRKDNTFYYYNKQRECEEKEVHKVKVYAY 88

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BLAST of Meiosis expressed protein 1 vs. Ensembl Mouse
Match: Meig1 (meiosis expressed gene 1 [Source:MGI Symbol;Acc:MGI:1202878])

HSP 1 Score: 94.3597 bits (233), Expect = 6.100e-26
Identity = 40/84 (47.62%), Postives = 64/84 (76.19%), Query Frame = 1

Query:   67 DPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRG-HVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
            D KP ++ RAK W+ ++EN YRFQ  GYRDE+EY+ ++   +V++WP++G+VKK+ R+D  F Y++K+REC +K++ K K+Y Y
Sbjct:    5 DVKPKSISRAKKWSEEIENLYRFQQAGYRDEIEYKQVKQVAMVDRWPETGYVKKLQRRDNTFFYYNKERECEDKEVHKVKVYVY 88

[Close]

Query:   67 DPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRG-HVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
            D KP ++ RAK W+ ++EN YRFQ  GYRDE+EY+ ++   +V++WP++G+VKK+ R+D  F Y++K+REC +K++ K K+Y Y
Sbjct:    5 DVKPKSISRAKKWSEEIENLYRFQQAGYRDEIEYKQVKQVAMVDRWPETGYVKKLQRRDNTFFYYNKERECEDKEVHKVKVYVY 88

[Close]

Query:   67 DPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRG-HVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
            D KP ++ RAK W+ ++EN YRFQ  GYRDE+EY+ ++   +V++WP++G+VKK+ R+D  F Y++K+REC +K++ K K+Y Y
Sbjct:    5 DVKPKSISRAKKWSEEIENLYRFQQAGYRDEIEYKQVKQVAMVDRWPETGYVKKLQRRDNTFFYYNKERECEDKEVHKVKVYVY 88

[Close]

Query:   67 DPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRG-HVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
            D KP ++ RAK W+ ++EN YRFQ  GYRDE+EY+ ++   +V++WP++G+VKK+ R+D  F Y++K+REC +K++ K K+Y Y
Sbjct:    5 DVKPKSISRAKKWSEEIENLYRFQQAGYRDEIEYKQVKQVAMVDRWPETGYVKKLQRRDNTFFYYNKERECEDKEVHKVKVYVY 88

[Close]

Query:   67 DPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRG-HVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
            D KP ++ RAK W+ ++EN YRFQ  GYRDE+EY+ ++   +V++WP++G+VKK+ R+D  F Y++K+REC +K++ K K+Y Y
Sbjct:    5 DVKPKSISRAKKWSEEIENLYRFQQAGYRDEIEYKQVKQVAMVDRWPETGYVKKLQRRDNTFFYYNKERECEDKEVHKVKVYVY 88

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BLAST of Meiosis expressed protein 1 vs. UniProt/SwissProt
Match: sp|Q6PB04|MEIG1_XENLA (Meiosis expressed gene 1 protein homolog OS=Xenopus laevis OX=8355 GN=meig1 PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 1.232e-27
Identity = 44/84 (52.38%), Postives = 63/84 (75.00%), Query Frame = 1

Query:   67 DPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRG-HVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
            + KP +M RAK W+ ++EN YRFQ  GYRDEVEY+ ++   +V++WP++GFVKK+ RKD  F Y++K REC EK++ K K+Y Y
Sbjct:    5 EAKPKSMTRAKQWSDEIENLYRFQTAGYRDEVEYRHVKQVEMVDRWPETGFVKKLQRKDNTFYYYNKKRECEEKEVNKVKVYAY 88

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BLAST of Meiosis expressed protein 1 vs. UniProt/SwissProt
Match: sp|Q5RGE4|MEIG1_DANRE (Meiosis expressed gene 1 protein homolog OS=Danio rerio OX=7955 GN=meig1 PE=3 SV=2)

HSP 1 Score: 98.2117 bits (243), Expect = 1.537e-26
Identity = 41/83 (49.40%), Postives = 61/83 (73.49%), Query Frame = 1

Query:   67 DPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRGHVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
            + KP +M RAK W  +VEN YRFQ  GYRDE+EY+ I+   +++WPD+GFVKK+ R+D  F Y+++ REC ++++ K K+Y Y
Sbjct:   10 NAKPKSMSRAKQWTGEVENLYRFQQAGYRDELEYRQIKQAEIDRWPDTGFVKKLQRRDNTFYYYNRKRECEDREVHKVKVYAY 92

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BLAST of Meiosis expressed protein 1 vs. UniProt/SwissProt
Match: sp|A7SMR0|MEIG1_NEMVE (Meiosis expressed gene 1 protein homolog OS=Nematostella vectensis OX=45351 GN=v1g237368 PE=3 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 2.116e-26
Identity = 48/90 (53.33%), Postives = 61/90 (67.78%), Query Frame = 1

Query:   46 TTMANKVDPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRGHVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
            T +A K  PK V+  R K W+ +VE AYRFQ  GYRD +EY  I+    E+WP +G+VKK+ RKDG F YFDK REC +KD+ K K+Y Y
Sbjct:    2 TAVAQKCGPKSVS--RPKHWSEEVEEAYRFQCAGYRDAIEYSDIKQKEPERWPHNGYVKKLQRKDGCFVYFDKTRECQDKDVNKTKMYGY 89

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BLAST of Meiosis expressed protein 1 vs. UniProt/SwissProt
Match: sp|Q32LI5|MEIG1_BOVIN (Meiosis expressed gene 1 protein homolog OS=Bos taurus OX=9913 GN=MEIG1 PE=3 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 9.896e-26
Identity = 43/89 (48.31%), Postives = 66/89 (74.16%), Query Frame = 1

Query:   52 MANKVDPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRG-HVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
            MAN  D KP ++ RAK W+ ++EN YRFQ  GYRDE+EY+ ++   +V++WP++G+VKK+ R+D  F Y++K REC +K++ K K+Y Y
Sbjct:    1 MANS-DVKPKSISRAKKWSEEIENLYRFQQAGYRDEIEYKQVKQVSMVDRWPETGYVKKLQRRDNTFYYYNKQRECDDKEVHKVKIYAY 88

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BLAST of Meiosis expressed protein 1 vs. UniProt/SwissProt
Match: sp|Q61845|MEIG1_MOUSE (Meiosis-expressed gene 1 protein OS=Mus musculus OX=10090 GN=Meig1 PE=1 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 4.267e-25
Identity = 40/84 (47.62%), Postives = 64/84 (76.19%), Query Frame = 1

Query:   67 DPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRG-HVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
            D KP ++ RAK W+ ++EN YRFQ  GYRDE+EY+ ++   +V++WP++G+VKK+ R+D  F Y++K+REC +K++ K K+Y Y
Sbjct:    5 DVKPKSISRAKKWSEEIENLYRFQQAGYRDEIEYKQVKQVAMVDRWPETGYVKKLQRRDNTFFYYNKERECEDKEVHKVKVYVY 88

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BLAST of Meiosis expressed protein 1 vs. TrEMBL
Match: A0A5J4NUB2 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0001190 PE=4 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 4.852e-31
Identity = 54/84 (64.29%), Postives = 65/84 (77.38%), Query Frame = 1

Query:   73 KPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRGHVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTYDEK 324
            KP  M RAK W+ +VENAYRFQL GYRDE+EY   +   VEKW  SGFVKK+ RKDG F YFD+ RECP+KDI KCKLY+Y+++
Sbjct:    5 KPCGMCRAKYWSSEVENAYRFQLAGYRDELEYTEHQADQVEKWTGSGFVKKLRRKDGSFYYFDQKRECPDKDINKCKLYSYEDR 88

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BLAST of Meiosis expressed protein 1 vs. TrEMBL
Match: A0A504YJI8 (Meiosis expressed protein 1 OS=Fasciola gigantica OX=46835 GN=FGIG_12548 PE=4 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 6.646e-31
Identity = 51/82 (62.20%), Postives = 65/82 (79.27%), Query Frame = 1

Query:   73 KPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRGHVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTYD 318
            +P +M RA +W  +VENAYR+Q+ GYRDE+EY +I+   VEKW  SGFVKK+ RKDGYF YFD+ REC +KD+ KCKLYTY+
Sbjct:    6 QPSSMRRALNWTSEVENAYRYQIAGYRDEIEYYSIQPEQVEKWTTSGFVKKLRRKDGYFYYFDQARECSDKDVKKCKLYTYE 87

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BLAST of Meiosis expressed protein 1 vs. TrEMBL
Match: A0A2G8KQY3 (Putative meiosis expressed protein 1 protein-like OS=Stichopus japonicus OX=307972 GN=BSL78_12651 PE=4 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 5.785e-30
Identity = 49/88 (55.68%), Postives = 69/88 (78.41%), Query Frame = 1

Query:   55 ANKVDPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRGHVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTYD 318
            A  V P+P +M RAK W+ +VE AYRFQ+ GY+ E EY A++G  VE+WPDSG++KK+ RKDG F Y++KDRECP+KD+ + K+Y+Y+
Sbjct:    3 AASVIPEPKSMTRAKKWSDEVEEAYRFQVAGYKGEAEYTALKGLEVERWPDSGYIKKLTRKDGNFYYYNKDRECPDKDVHQIKIYSYN 90

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BLAST of Meiosis expressed protein 1 vs. TrEMBL
Match: A0A2H1CXD7 (Meiosis expressed protein 1 OS=Fasciola hepatica OX=6192 GN=D915_000103 PE=4 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 7.974e-30
Identity = 50/89 (56.18%), Postives = 65/89 (73.03%), Query Frame = 1

Query:   52 MANKVDPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRGHVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTYD 318
            M  +   +P +M RA +W  +VENAYR+Q  GYRDE+EY + +   VEKW  SGFVKK+ R+DGYF YFD+ REC +KD+ KCKLYTY+
Sbjct:    1 MPMRTMKQPSSMRRALNWTSEVENAYRYQTAGYRDEIEYYSFQPEQVEKWTTSGFVKKLRRRDGYFYYFDQARECSDKDVKKCKLYTYE 89

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BLAST of Meiosis expressed protein 1 vs. TrEMBL
Match: A0A267H7B5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig024898g1 PE=4 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 1.213e-29
Identity = 49/88 (55.68%), Postives = 67/88 (76.14%), Query Frame = 1

Query:   52 MANKVDPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRGHVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
            MA+ V P+P  ++R K+WN +VE+AYRFQL GYRD  EY+A+RG    +W ++GFVKK+ R+DG F YF++ REC +KD+ KCKLY Y
Sbjct:    1 MASSVAPQPTRLVRPKNWNEEVEDAYRFQLAGYRDAAEYRALRGEEPLRWTETGFVKKLTRRDGCFYYFNRLRECLDKDVHKCKLYCY 88

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BLAST of Meiosis expressed protein 1 vs. Ensembl Cavefish
Match: meig1 (meiosis/spermiogenesis associated 1 [Source:NCBI gene;Acc:103040144])

HSP 1 Score: 93.5893 bits (231), Expect = 9.828e-26
Identity = 40/82 (48.78%), Postives = 59/82 (71.95%), Query Frame = 1

Query:   70 PKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRGHVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
             KP +M RAK W  +VEN YRFQ  GYRDE+EY+ I+   ++ WP++GFVKK+ R+D  + Y+++ REC +K++ K K+Y Y
Sbjct:    5 TKPKSMSRAKQWTDEVENLYRFQQAGYRDEMEYRQIKQLEIDWWPETGFVKKLQRRDNTYYYYNRKRECEDKEVHKVKMYAY 86

[Close]

BLAST of Meiosis expressed protein 1 vs. Ensembl Cavefish
Match: meig1 (meiosis/spermiogenesis associated 1 [Source:NCBI gene;Acc:103040144])

HSP 1 Score: 93.2041 bits (230), Expect = 3.055e-25
Identity = 40/82 (48.78%), Postives = 59/82 (71.95%), Query Frame = 1

Query:   70 PKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRGHVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
             KP +M RAK W  +VEN YRFQ  GYRDE+EY+ I+   ++ WP++GFVKK+ R+D  + Y+++ REC +K++ K K+Y Y
Sbjct:   38 TKPKSMSRAKQWTDEVENLYRFQQAGYRDEMEYRQIKQLEIDWWPETGFVKKLQRRDNTYYYYNRKRECEDKEVHKVKMYAY 119

[Close]

BLAST of Meiosis expressed protein 1 vs. Ensembl Sea Lamprey
Match: meig1 (meiosis/spermiogenesis associated 1 [Source:NCBI gene;Acc:541343])

HSP 1 Score: 90.5077 bits (223), Expect = 2.804e-25
Identity = 40/81 (49.38%), Postives = 58/81 (71.60%), Query Frame = 1

Query:   76 PVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRGHV-VEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
            P  M RA+SW+  VE+ YRFQ  GYRDE EY+ +R    V++WPD+G+VKK+ R+D  F Y+++ REC +K++ K K+Y Y
Sbjct:    7 PKTMSRARSWSEVVEDLYRFQQAGYRDETEYRLVRNVAEVDRWPDTGYVKKLQRRDNTFYYYNRQRECEDKEVHKVKVYAY 87

[Close]

BLAST of Meiosis expressed protein 1 vs. Ensembl Nematostella
Match: EDO35032 (Meiosis expressed gene 1 protein homolog [Source:UniProtKB/Swiss-Prot;Acc:A7SMR0])

HSP 1 Score: 97.4413 bits (241), Expect = 8.672e-28
Identity = 48/90 (53.33%), Postives = 61/90 (67.78%), Query Frame = 1

Query:   46 TTMANKVDPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRGHVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTY 315
            T +A K  PK V+  R K W+ +VE AYRFQ  GYRD +EY  I+    E+WP +G+VKK+ RKDG F YFDK REC +KD+ K K+Y Y
Sbjct:    2 TAVAQKCGPKSVS--RPKHWSEEVEEAYRFQCAGYRDAIEYSDIKQKEPERWPHNGYVKKLQRKDGCFVYFDKTRECQDKDVNKTKMYGY 89

[Close]

BLAST of Meiosis expressed protein 1 vs. Planmine SMEST
Match: SMESG000031493.1 (SMESG000031493.1)

HSP 1 Score: 190.66 bits (483), Expect = 3.401e-64
Identity = 91/92 (98.91%), Postives = 91/92 (98.91%), Query Frame = 1

Query:   52 MANKVDPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRGHVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTYDEKK 327
            MANKVDPKPVAMIRAKSWN DVENAYRFQLGGYRDEVEYQAIRGHVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTYDEKK
Sbjct:    1 MANKVDPKPVAMIRAKSWNTDVENAYRFQLGGYRDEVEYQAIRGHVVEKWPDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTYDEKK 92

The following BLAST results are available for this feature:

BLAST of Meiosis expressed protein 1 vs. Ensembl Human
BLAST of Meiosis expressed protein 1 vs. Ensembl Celegans
BLAST of Meiosis expressed protein 1 vs. Ensembl Fly
BLAST of Meiosis expressed protein 1 vs. Ensembl Zebrafish
BLAST of Meiosis expressed protein 1 vs. Ensembl Xenopus
BLAST of Meiosis expressed protein 1 vs. Ensembl Mouse
BLAST of Meiosis expressed protein 1 vs. UniProt/SwissProt
BLAST of Meiosis expressed protein 1 vs. TrEMBL
BLAST of Meiosis expressed protein 1 vs. Ensembl Cavefish
BLAST of Meiosis expressed protein 1 vs. Ensembl Sea Lamprey
BLAST of Meiosis expressed protein 1 vs. Ensembl Yeast
BLAST of Meiosis expressed protein 1 vs. Ensembl Nematostella
BLAST of Meiosis expressed protein 1 vs. Ensembl Medaka
BLAST of Meiosis expressed protein 1 vs. Planmine SMEST

BLAST of Meiosis expressed protein 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2


Match Name	E-value	Identity	Description
MEIG1	2.214e-24	46.43	meiosis/spermiogenesis associated 1 [Source:HGNC S...	[more]
MEIG1	2.214e-24	46.43	meiosis/spermiogenesis associated 1 [Source:HGNC S...	[more]

BLAST of Meiosis expressed protein 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Meiosis expressed protein 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Meiosis expressed protein 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4


Match Name	E-value	Identity	Description
meig1	2.036e-27	49.40	meiosis/spermiogenesis associated 1 [Source:NCBI g...	[more]
meig1	2.036e-27	49.40	meiosis/spermiogenesis associated 1 [Source:NCBI g...	[more]
meig1	2.036e-27	49.40	meiosis/spermiogenesis associated 1 [Source:NCBI g...	[more]
meig1	2.036e-27	49.40	meiosis/spermiogenesis associated 1 [Source:NCBI g...	[more]

BLAST of Meiosis expressed protein 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1


Match Name	E-value	Identity	Description
b3gnt4	1.849e-28	52.38	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltr...	[more]

BLAST of Meiosis expressed protein 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Meig1	6.100e-26	47.62	meiosis expressed gene 1 [Source:MGI Symbol;Acc:MG...	[more]
Meig1	6.100e-26	47.62	meiosis expressed gene 1 [Source:MGI Symbol;Acc:MG...	[more]
Meig1	6.100e-26	47.62	meiosis expressed gene 1 [Source:MGI Symbol;Acc:MG...	[more]
Meig1	6.100e-26	47.62	meiosis expressed gene 1 [Source:MGI Symbol;Acc:MG...	[more]
Meig1	6.100e-26	47.62	meiosis expressed gene 1 [Source:MGI Symbol;Acc:MG...	[more]

BLAST of Meiosis expressed protein 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q6PB04\|MEIG1_XENLA	1.232e-27	52.38	Meiosis expressed gene 1 protein homolog OS=Xenopu...	[more]
sp\|Q5RGE4\|MEIG1_DANRE	1.537e-26	49.40	Meiosis expressed gene 1 protein homolog OS=Danio ...	[more]
sp\|A7SMR0\|MEIG1_NEMVE	2.116e-26	53.33	Meiosis expressed gene 1 protein homolog OS=Nemato...	[more]
sp\|Q32LI5\|MEIG1_BOVIN	9.896e-26	48.31	Meiosis expressed gene 1 protein homolog OS=Bos ta...	[more]
sp\|Q61845\|MEIG1_MOUSE	4.267e-25	47.62	Meiosis-expressed gene 1 protein OS=Mus musculus O...	[more]

BLAST of Meiosis expressed protein 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A5J4NUB2	4.852e-31	64.29	Uncharacterized protein OS=Paragonimus westermani ...	[more]
A0A504YJI8	6.646e-31	62.20	Meiosis expressed protein 1 OS=Fasciola gigantica ...	[more]
A0A2G8KQY3	5.785e-30	55.68	Putative meiosis expressed protein 1 protein-like ...	[more]
A0A2H1CXD7	7.974e-30	56.18	Meiosis expressed protein 1 OS=Fasciola hepatica O...	[more]
A0A267H7B5	1.213e-29	55.68	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]

BLAST of Meiosis expressed protein 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
meig1	9.828e-26	48.78	meiosis/spermiogenesis associated 1 [Source:NCBI g...	[more]
meig1	3.055e-25	48.78	meiosis/spermiogenesis associated 1 [Source:NCBI g...	[more]

BLAST of Meiosis expressed protein 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
meig1	2.804e-25	49.38	meiosis/spermiogenesis associated 1 [Source:NCBI g...	[more]

BLAST of Meiosis expressed protein 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Meiosis expressed protein 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO35032	8.672e-28	53.33	Meiosis expressed gene 1 protein homolog [Source:...	[more]

BLAST of Meiosis expressed protein 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Meiosis expressed protein 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000031493.1	3.401e-64	98.91	SMESG000031493.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30015563 ID=SMED30015563|Name=Meiosis expressed protein 1|organism=Schmidtea mediterranea sexual|type=transcript|length=400bp
GATTTTGCTCTAATCATTGCTACATAGCAACGGAAAAATATTTAAACTAC
TATGGCAAATAAAGTAGATCCTAAACCAGTAGCAATGATTAGAGCAAAAT
CTTGGAATATTGATGTCGAAAATGCCTATCGATTTCAGTTAGGTGGATAC
AGAGATGAAGTTGAATATCAAGCAATAAGAGGGCATGTCGTTGAAAAATG
GCCTGACAGTGGGTTTGTTAAAAAAATACACAGAAAAGATGGTTATTTCA
ATTATTTTGACAAAGATCGAGAATGTCCCGAAAAAGATATTCCAAAGTGC
AAATTATACACTTATGATGAAAAAAAAATAAGAAAATTCATTTACTTTAT
TCGTTTAACACCTTATTGGTAATGTAAATATATTGTTTCCTACTTTGAAA

protein sequence of SMED30015563-orf-1

>SMED30015563-orf-1 ID=SMED30015563-orf-1|Name=SMED30015563-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=107bp
MANKVDPKPVAMIRAKSWNIDVENAYRFQLGGYRDEVEYQAIRGHVVEKW
PDSGFVKKIHRKDGYFNYFDKDRECPEKDIPKCKLYTYDEKKIRKFIYFI
RLTPYW*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000020	protonephridia
PLANA:0000099	neuron
PLANA:0000418	head
PLANA:0002032	epidermal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR020186	Meiosis-expressed_gene_1

Vocabulary: **cellular component**
Term	Definition
GO:0005634	nucleus

Main menu

Meiosis expressed protein 1

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 11

Owlery

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