60S ribosomal protein L21

Overview
Name60S ribosomal protein L21
Smed IDSMED30015534
Length (bp)573
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 60S ribosomal protein L21 (SMED30015534) t-SNE clustered cells

Violin plots show distribution of expression levels for 60S ribosomal protein L21 (SMED30015534) in cells (dots) of each of the 12 neoblast clusters.

 

back to top


 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 60S ribosomal protein L21 (SMED30015534) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 60S ribosomal protein L21 (SMED30015534) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top


 

Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30015534

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 16

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30015534h1SMcG0010658 SmedASXL_018651SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
pharynxSMED30015534h1SMcG0010658 dd_Smed_v4_189_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30015534h1SMcG0010658 dd_Smed_v4_189_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30015534h1SMcG0010658 dd_Smed_v4_189_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30015534h1SMcG0010658 dd_Smed_v4_189_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30015534h1SMcG0010658 dd_Smed_v4_189_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30015534h1SMcG0010658 dd_Smed_v4_189_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30015534h1SMcG0010658 dd_Smed_v4_189_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30015534h1SMcG0010658 dd_Smed_v4_189_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30015534h1SMcG0010658 dd_Smed_v4_189_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30015534h1SMcG0010658 dd_Smed_v4_189_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30015534h1SMcG0010658 dd_Smed_v4_189_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gamma neoblastSMED30015534h1SMcG0010658 dd_Smed_v6_189_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30015534h1SMcG0010658 dd_Smed_v6_189_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 4 cellSMED30015534h1SMcG0010658 H.18.1bsmed_ncbi_20240424PMID:18786419
Eisenhoffer et al., 2008
whole organism asexual adult colorimetric in situ hybridization evidence
Category 4 cellSMED30015534h1SMcG0010658 H.18.1bsmed_ncbi_20240424PMID:18786419
Eisenhoffer et al., 2008
whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
BLAST of 60S ribosomal protein L21 vs. Ensembl Human
Match: RPL21 (ribosomal protein L21 [Source:HGNC Symbol;Acc:HGNC:10313])

HSP 1 Score: 214.927 bits (546), Expect = 1.771e-71
Identity = 98/160 (61.25%), Postives = 127/160 (79.38%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NTKG RRGTRY+F + FRKHG +P +T++ +YK+GD VDIKG G+VQKGMPHKCYHG+TGRVYNVTQHA+G++VNK+V  +I+ K+INVRIEH+K SK R+ FL RVK+ND+ K+ AKE GT++ LKR PA P  AH V+T       L+P+PY+ +A
Sbjct:    1 MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIVVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA 160          
BLAST of 60S ribosomal protein L21 vs. Ensembl Human
Match: RPL21 (ribosomal protein L21 [Source:HGNC Symbol;Acc:HGNC:10313])

HSP 1 Score: 214.927 bits (546), Expect = 1.771e-71
Identity = 98/160 (61.25%), Postives = 127/160 (79.38%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NTKG RRGTRY+F + FRKHG +P +T++ +YK+GD VDIKG G+VQKGMPHKCYHG+TGRVYNVTQHA+G++VNK+V  +I+ K+INVRIEH+K SK R+ FL RVK+ND+ K+ AKE GT++ LKR PA P  AH V+T       L+P+PY+ +A
Sbjct:    1 MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIVVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA 160          
BLAST of 60S ribosomal protein L21 vs. Ensembl Human
Match: RPL21 (ribosomal protein L21 [Source:HGNC Symbol;Acc:HGNC:10313])

HSP 1 Score: 214.927 bits (546), Expect = 1.771e-71
Identity = 98/160 (61.25%), Postives = 127/160 (79.38%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NTKG RRGTRY+F + FRKHG +P +T++ +YK+GD VDIKG G+VQKGMPHKCYHG+TGRVYNVTQHA+G++VNK+V  +I+ K+INVRIEH+K SK R+ FL RVK+ND+ K+ AKE GT++ LKR PA P  AH V+T       L+P+PY+ +A
Sbjct:    1 MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIVVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA 160          
BLAST of 60S ribosomal protein L21 vs. Ensembl Human
Match: RPL21 (ribosomal protein L21 [Source:HGNC Symbol;Acc:HGNC:10313])

HSP 1 Score: 214.927 bits (546), Expect = 1.771e-71
Identity = 98/160 (61.25%), Postives = 127/160 (79.38%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NTKG RRGTRY+F + FRKHG +P +T++ +YK+GD VDIKG G+VQKGMPHKCYHG+TGRVYNVTQHA+G++VNK+V  +I+ K+INVRIEH+K SK R+ FL RVK+ND+ K+ AKE GT++ LKR PA P  AH V+T       L+P+PY+ +A
Sbjct:    1 MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIVVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA 160          
BLAST of 60S ribosomal protein L21 vs. Ensembl Human
Match: RPL21 (ribosomal protein L21 [Source:HGNC Symbol;Acc:HGNC:10313])

HSP 1 Score: 176.792 bits (447), Expect = 6.729e-57
Identity = 81/121 (66.94%), Postives = 103/121 (85.12%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKE 432
            M NTKG RRGTRY+F + FRKHG +P +T++ +YK+GD VDIKG G+VQKGMPHKCYHG+TGRVYNVTQHA+G++VNK+V  +I+ K+INVRIEH+K SK R+ FL RVK+ND+ K+ AKE
Sbjct:    1 MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIVVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKE 121          
BLAST of 60S ribosomal protein L21 vs. Ensembl Celegans
Match: rpl-21 (60S ribosomal protein L21 [Source:UniProtKB/Swiss-Prot;Acc:P34334])

HSP 1 Score: 200.675 bits (509), Expect = 3.040e-66
Identity = 95/161 (59.01%), Postives = 121/161 (75.16%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIP-LKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M N+KG RRGTRY+F ++FRKHG    ST+ T YKRGD VDIK +G+ QKGMP K YHGRTGR++NVT+ A+G+IVNKRV   I+PK+IN+RIEH+K SKCR DFLNRVK NDE ++ AK  G  +P LKR+P  P  AH V T+NN    L P+ ++++A
Sbjct:    1 MTNSKGLRRGTRYMFARDFRKHGVEHLSTYYTQYKRGDLVDIKTNGAFQKGMPFKAYHGRTGRIFNVTRGAVGIIVNKRVRGNILPKRINIRIEHIKPSKCRTDFLNRVKSNDEKRKAAKSAGQPVPALKRLPVAPRGAHTVTTQNNEPELLAPLRFEIVA 161          
BLAST of 60S ribosomal protein L21 vs. Ensembl Fly
Match: RpL21 (gene:FBgn0032987 transcript:FBtr0085961)

HSP 1 Score: 206.068 bits (523), Expect = 2.845e-68
Identity = 97/160 (60.62%), Postives = 124/160 (77.50%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M N+KGYRRGTR +F + FRKHG +P ST++ V+K GD VDIKG G+VQKG+P+K YHG+TGR++NVTQHA+GVIVNKRV  +I+ K++NVRIEH+  SKCREDFL RVK+N+ + + AKE G ++ LKR P QP  AH VK K    I L P+PY+ IA
Sbjct:    1 MTNSKGYRRGTRDMFSRPFRKHGVIPLSTYMRVFKIGDIVDIKGHGAVQKGLPYKAYHGKTGRIFNVTQHAVGVIVNKRVRGKILAKRVNVRIEHIHHSKCREDFLRRVKENERLLKEAKEKGQWVSLKRQPEQPKKAHFVK-KLEEPIALAPIPYEFIA 159          
BLAST of 60S ribosomal protein L21 vs. Ensembl Fly
Match: RpL21 (gene:FBgn0032987 transcript:FBtr0345541)

HSP 1 Score: 206.068 bits (523), Expect = 2.845e-68
Identity = 97/160 (60.62%), Postives = 124/160 (77.50%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M N+KGYRRGTR +F + FRKHG +P ST++ V+K GD VDIKG G+VQKG+P+K YHG+TGR++NVTQHA+GVIVNKRV  +I+ K++NVRIEH+  SKCREDFL RVK+N+ + + AKE G ++ LKR P QP  AH VK K    I L P+PY+ IA
Sbjct:    1 MTNSKGYRRGTRDMFSRPFRKHGVIPLSTYMRVFKIGDIVDIKGHGAVQKGLPYKAYHGKTGRIFNVTQHAVGVIVNKRVRGKILAKRVNVRIEHIHHSKCREDFLRRVKENERLLKEAKEKGQWVSLKRQPEQPKKAHFVK-KLEEPIALAPIPYEFIA 159          
BLAST of 60S ribosomal protein L21 vs. Ensembl Fly
Match: RpL21 (gene:FBgn0032987 transcript:FBtr0331295)

HSP 1 Score: 206.068 bits (523), Expect = 2.845e-68
Identity = 97/160 (60.62%), Postives = 124/160 (77.50%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M N+KGYRRGTR +F + FRKHG +P ST++ V+K GD VDIKG G+VQKG+P+K YHG+TGR++NVTQHA+GVIVNKRV  +I+ K++NVRIEH+  SKCREDFL RVK+N+ + + AKE G ++ LKR P QP  AH VK K    I L P+PY+ IA
Sbjct:    1 MTNSKGYRRGTRDMFSRPFRKHGVIPLSTYMRVFKIGDIVDIKGHGAVQKGLPYKAYHGKTGRIFNVTQHAVGVIVNKRVRGKILAKRVNVRIEHIHHSKCREDFLRRVKENERLLKEAKEKGQWVSLKRQPEQPKKAHFVK-KLEEPIALAPIPYEFIA 159          
BLAST of 60S ribosomal protein L21 vs. Ensembl Zebrafish
Match: rpl21 (ribosomal protein L21 [Source:ZFIN;Acc:ZDB-GENE-030131-8512])

HSP 1 Score: 204.912 bits (520), Expect = 9.997e-68
Identity = 100/160 (62.50%), Postives = 128/160 (80.00%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NT+G RRGTRY+F + FRKHG +P ST++ +YK+GD VDIKG G++QKGMPHKCYHG+TGRVYNVTQHA+G+IVNK+V  +I+ K+INVRIEH+K SK R+ FL RVK+N++ K  AK+ GT+I LKR PA P  AH V TKNN    L+P+PY+ +A
Sbjct:    1 MTNTRGKRRGTRYMFARPFRKHGPIPLSTYMRIYKKGDIVDIKGTGTIQKGMPHKCYHGKTGRVYNVTQHAVGIIVNKQVKGKILAKRINVRIEHIKHSKSRDSFLQRVKENEKKKVEAKKEGTWIELKRQPAAPRPAHFVSTKNNKPQLLEPIPYEFMA 160          
BLAST of 60S ribosomal protein L21 vs. Ensembl Zebrafish
Match: rpl21 (ribosomal protein L21 [Source:ZFIN;Acc:ZDB-GENE-030131-8512])

HSP 1 Score: 204.912 bits (520), Expect = 9.997e-68
Identity = 100/160 (62.50%), Postives = 128/160 (80.00%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NT+G RRGTRY+F + FRKHG +P ST++ +YK+GD VDIKG G++QKGMPHKCYHG+TGRVYNVTQHA+G+IVNK+V  +I+ K+INVRIEH+K SK R+ FL RVK+N++ K  AK+ GT+I LKR PA P  AH V TKNN    L+P+PY+ +A
Sbjct:    1 MTNTRGKRRGTRYMFARPFRKHGPIPLSTYMRIYKKGDIVDIKGTGTIQKGMPHKCYHGKTGRVYNVTQHAVGIIVNKQVKGKILAKRINVRIEHIKHSKSRDSFLQRVKENEKKKVEAKKEGTWIELKRQPAAPRPAHFVSTKNNKPQLLEPIPYEFMA 160          
BLAST of 60S ribosomal protein L21 vs. Ensembl Zebrafish
Match: rpl21 (ribosomal protein L21 [Source:ZFIN;Acc:ZDB-GENE-030131-8512])

HSP 1 Score: 204.912 bits (520), Expect = 9.997e-68
Identity = 100/160 (62.50%), Postives = 128/160 (80.00%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NT+G RRGTRY+F + FRKHG +P ST++ +YK+GD VDIKG G++QKGMPHKCYHG+TGRVYNVTQHA+G+IVNK+V  +I+ K+INVRIEH+K SK R+ FL RVK+N++ K  AK+ GT+I LKR PA P  AH V TKNN    L+P+PY+ +A
Sbjct:    1 MTNTRGKRRGTRYMFARPFRKHGPIPLSTYMRIYKKGDIVDIKGTGTIQKGMPHKCYHGKTGRVYNVTQHAVGIIVNKQVKGKILAKRINVRIEHIKHSKSRDSFLQRVKENEKKKVEAKKEGTWIELKRQPAAPRPAHFVSTKNNKPQLLEPIPYEFMA 160          
BLAST of 60S ribosomal protein L21 vs. Ensembl Zebrafish
Match: AL929009.3 (pep chromosome:GRCz11:24:21603588:21637183:-1 gene:ENSDARG00000113148.1 transcript:ENSDART00000189379.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:AL929009.3)

HSP 1 Score: 47.3654 bits (111), Expect = 1.663e-7
Identity = 22/57 (38.60%), Postives = 35/57 (61.40%), Query Frame = 1
Query:  235 CYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKN 405
            C+HG+   +YNVTQ   G ++NK + +  +  + N+RIE  + SK  + F+ RVK N
Sbjct:    1 CHHGKDKSIYNVTQIGYGPLMNKEILSNALSIQFNIRIESFRHSKTHKTFVERVKFN 57          
BLAST of 60S ribosomal protein L21 vs. Ensembl Xenopus
Match: rpl21 (ribosomal protein L21 [Source:NCBI gene;Acc:448539])

HSP 1 Score: 196.052 bits (497), Expect = 4.453e-64
Identity = 95/160 (59.38%), Postives = 125/160 (78.12%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NT+G RRGTRY+F + FRKHG +P ST++ +YK+GD VDIKG G++QKGMPHKCYHG+TGRVYNVTQHA+G+IVNK++  RI+ K+INVR+EH++ SK R+ FL RVK+N+  K+ AKE G ++ LKR PAQP  AH V T       L+P+PY+ +A
Sbjct:    1 MTNTRGKRRGTRYMFSRPFRKHGVVPLSTYMRIYKKGDIVDIKGMGTIQKGMPHKCYHGKTGRVYNVTQHAVGIIVNKQIKGRILAKRINVRVEHIRHSKSRDSFLQRVKENERKKKEAKEKGIWVELKRQPAQPREAHFVTTGGVEPQLLEPIPYEFMA 160          
BLAST of 60S ribosomal protein L21 vs. Ensembl Mouse
Match: Rpl21 (ribosomal protein L21 [Source:MGI Symbol;Acc:MGI:1278340])

HSP 1 Score: 215.698 bits (548), Expect = 7.114e-72
Identity = 99/160 (61.88%), Postives = 127/160 (79.38%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NTKG RRGTRY+F + FRKHG +P +T++ +YK+GD VDIKG G+VQKGMPHKCYHG+TGRVYNVTQHA+G+IVNK+V  +I+ K+INVRIEH+K SK R+ FL RVK+ND+ K+ AKE GT++ LKR PA P  AH V+T       L+P+PY+ +A
Sbjct:    1 MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIIVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA 160          
BLAST of 60S ribosomal protein L21 vs. Ensembl Mouse
Match: Rpl21 (ribosomal protein L21 [Source:MGI Symbol;Acc:MGI:1278340])

HSP 1 Score: 215.698 bits (548), Expect = 7.114e-72
Identity = 99/160 (61.88%), Postives = 127/160 (79.38%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NTKG RRGTRY+F + FRKHG +P +T++ +YK+GD VDIKG G+VQKGMPHKCYHG+TGRVYNVTQHA+G+IVNK+V  +I+ K+INVRIEH+K SK R+ FL RVK+ND+ K+ AKE GT++ LKR PA P  AH V+T       L+P+PY+ +A
Sbjct:    1 MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIIVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA 160          
BLAST of 60S ribosomal protein L21 vs. Ensembl Mouse
Match: Rpl21 (ribosomal protein L21 [Source:MGI Symbol;Acc:MGI:1278340])

HSP 1 Score: 215.698 bits (548), Expect = 7.114e-72
Identity = 99/160 (61.88%), Postives = 127/160 (79.38%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NTKG RRGTRY+F + FRKHG +P +T++ +YK+GD VDIKG G+VQKGMPHKCYHG+TGRVYNVTQHA+G+IVNK+V  +I+ K+INVRIEH+K SK R+ FL RVK+ND+ K+ AKE GT++ LKR PA P  AH V+T       L+P+PY+ +A
Sbjct:    1 MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIIVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA 160          
BLAST of 60S ribosomal protein L21 vs. UniProt/SwissProt
Match: sp|P49666|RL21_PIG (60S ribosomal protein L21 OS=Sus scrofa OX=9823 GN=RPL21 PE=1 SV=3)

HSP 1 Score: 214.927 bits (546), Expect = 8.506e-71
Identity = 98/160 (61.25%), Postives = 127/160 (79.38%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NTKG RRGTRY+F + FRKHG +P +T++ +YK+GD VDIKG G+VQKGMPHKCYHG+TGRVYNVTQHA+G++VNK+V  +I+ K+INVRIEH+K SK R+ FL RVK+ND+ K+ AKE GT++ LKR PA P  AH V+T       L+P+PY+ +A
Sbjct:    1 MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIVVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA 160          
BLAST of 60S ribosomal protein L21 vs. UniProt/SwissProt
Match: sp|P46778|RL21_HUMAN (60S ribosomal protein L21 OS=Homo sapiens OX=9606 GN=RPL21 PE=1 SV=2)

HSP 1 Score: 214.927 bits (546), Expect = 8.506e-71
Identity = 98/160 (61.25%), Postives = 127/160 (79.38%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NTKG RRGTRY+F + FRKHG +P +T++ +YK+GD VDIKG G+VQKGMPHKCYHG+TGRVYNVTQHA+G++VNK+V  +I+ K+INVRIEH+K SK R+ FL RVK+ND+ K+ AKE GT++ LKR PA P  AH V+T       L+P+PY+ +A
Sbjct:    1 MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIVVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA 160          
BLAST of 60S ribosomal protein L21 vs. UniProt/SwissProt
Match: sp|A5JSS2|RL21_CAPHI (60S ribosomal protein L21 OS=Capra hircus OX=9925 GN=RPL21 PE=2 SV=1)

HSP 1 Score: 214.157 bits (544), Expect = 1.519e-70
Identity = 98/160 (61.25%), Postives = 127/160 (79.38%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NTKG RRGTRY+F + FRKHG +P +T++ +Y++GD VDIKG G+VQKGMPHKCYHG+TGRVYNVTQHA+G+IVNK+V  +I+ K+INVRIEH+K SK R+ FL RVK+ND+ K+ AKE GT++ LKR PA P  AH V+T       L+P+PY+ +A
Sbjct:    1 MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYRKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIIVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA 160          
BLAST of 60S ribosomal protein L21 vs. UniProt/SwissProt
Match: sp|Q6QN05|RL21_CHILA (60S ribosomal protein L21 OS=Chinchilla lanigera OX=34839 GN=RPL21 PE=2 SV=3)

HSP 1 Score: 213.772 bits (543), Expect = 2.929e-70
Identity = 97/160 (60.62%), Postives = 127/160 (79.38%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NTKG RRGTRY+F + +RKHG +P +T++ +YK+GD VDIKG G+VQKGMPHKCYHG+TGRVYNVTQHA+G++VNK+V  +I+ K+INVRIEH+K SK R+ FL RVK+ND+ K+ AKE GT++ LKR PA P  AH V+T       L+P+PY+ +A
Sbjct:    1 MTNTKGKRRGTRYMFSRPYRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIVVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA 160          
BLAST of 60S ribosomal protein L21 vs. UniProt/SwissProt
Match: sp|O09167|RL21_MOUSE (60S ribosomal protein L21 OS=Mus musculus OX=10090 GN=Rpl21 PE=1 SV=3)

HSP 1 Score: 211.46 bits (537), Expect = 1.861e-69
Identity = 98/160 (61.25%), Postives = 126/160 (78.75%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NTKG RRGTRY+F + FRKHG +P +T++ +YK+GD VDIKG G+VQKGMPHKCYHG+TGRVYNVTQHA+G+IVNK+V  +I+ K+INVRIEH+K SK R+ FL RVK+ND+ K+ AKE GT++ LKR  A P  AH V+T       L+P+PY+ +A
Sbjct:    1 MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIIVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRHAAPPREAHFVRTNGKEPELLEPIPYEFMA 160          
BLAST of 60S ribosomal protein L21 vs. TrEMBL
Match: A0A4E0RLY1 (Ribosomal protein rpl21 OS=Fasciola hepatica OX=6192 GN=D915_006821 PE=4 SV=1)

HSP 1 Score: 253.447 bits (646), Expect = 1.340e-83
Identity = 121/160 (75.62%), Postives = 137/160 (85.62%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NTKGYRRGTR+LF + FR+HG LPTS ++ VYKRGD VDIKGDG VQKGMPHK YHGRTGRVYNVT HALGVIVNKRVG RIIPK+INVRIEHVK SKCREDFL RVK+ND  ++ AK +GTF+ LKR PAQP  AHVVKTKNN+V+ + P+PY+LIA
Sbjct:    1 MTNTKGYRRGTRHLFSRSFRRHGQLPTSVYMRVYKRGDIVDIKGDGGVQKGMPHKVYHGRTGRVYNVTPHALGVIVNKRVGKRIIPKRINVRIEHVKPSKCREDFLRRVKENDIKRKAAKSSGTFVVLKRQPAQPRPAHVVKTKNNAVVTVHPLPYELIA 160          
BLAST of 60S ribosomal protein L21 vs. TrEMBL
Match: A0A504YDH9 (Ribosomal protein rpl21 OS=Fasciola gigantica OX=46835 GN=FGIG_09313 PE=4 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 1.029e-82
Identity = 120/160 (75.00%), Postives = 136/160 (85.00%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NTKGYRRGTR+LF + FR+HG LPTS ++ VYKRGD VDIKGDG VQKGMPHK YHGRTGRVYNVT HALGVIVNKRVG RIIPK+INVRIEHVK SKCREDFL RVK+ND  ++ AK +GTF+ LKR P QP  AHVVKTKNN+V+ + P+PY+LIA
Sbjct:    1 MTNTKGYRRGTRHLFSRSFRRHGQLPTSVYMRVYKRGDIVDIKGDGGVQKGMPHKVYHGRTGRVYNVTPHALGVIVNKRVGKRIIPKRINVRIEHVKPSKCREDFLRRVKENDIKRKTAKSSGTFVVLKRQPEQPRPAHVVKTKNNAVVTVHPLPYELIA 160          
BLAST of 60S ribosomal protein L21 vs. TrEMBL
Match: A0A183AY61 (Uncharacterized protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS11896 PE=4 SV=1)

HSP 1 Score: 249.21 bits (635), Expect = 6.996e-82
Identity = 119/160 (74.38%), Postives = 134/160 (83.75%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NTKGYRRGTR+LF + FR+HG LP S ++ VYKRGD VDIKGDG VQKGMPHKCYHGRTGRVYNVT HALGVIVNKRVG RIIPK+INVRIEHVK SKCREDFL RVK+ND  ++ AK  GT++ LKR PAQP  AHVVKTK N V+ + P+PY+LIA
Sbjct:    1 MTNTKGYRRGTRHLFSRAFRRHGQLPMSVYMRVYKRGDIVDIKGDGGVQKGMPHKCYHGRTGRVYNVTPHALGVIVNKRVGKRIIPKRINVRIEHVKPSKCREDFLRRVKENDLKRKEAKAAGTYVELKRQPAQPRPAHVVKTKTNPVVTVHPLPYELIA 160          
BLAST of 60S ribosomal protein L21 vs. TrEMBL
Match: A0A183MM13 (Uncharacterized protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS17088 PE=4 SV=1)

HSP 1 Score: 248.825 bits (634), Expect = 1.108e-81
Identity = 118/160 (73.75%), Postives = 136/160 (85.00%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NTKGYRRGTRYLF ++FR HG LP+S ++ VY+RGD VDIKGDG VQKGMPHK YHGRTGRVYNVT HA+G+IVNKRVGNRIIPK+INVRIEHVK SKCREDFL RVK+ND  +R+AKE   F+ LKR PAQP +AH VKTKNN V+ + P+PY+LIA
Sbjct:    1 MTNTKGYRRGTRYLFSRKFRHHGPLPSSVYMRVYRRGDIVDIKGDGGVQKGMPHKSYHGRTGRVYNVTPHAVGIIVNKRVGNRIIPKRINVRIEHVKPSKCREDFLCRVKENDIKRRIAKEINVFVQLKRQPAQPRTAHFVKTKNNPVVTVHPLPYELIA 160          
BLAST of 60S ribosomal protein L21 vs. TrEMBL
Match: A0A183RED5 (Uncharacterized protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 247.669 bits (631), Expect = 3.351e-81
Identity = 117/160 (73.12%), Postives = 135/160 (84.38%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NTKGYRRGTRYLF ++FR HG LP+S ++ VY+RGD VDIKGDG VQKGMPHK YHGRTGRVYNVT HA+G+IVNKRVGNRIIPK+INVRIEHVK SKCREDFL RVK+ND  +R AKE   F+ LKR PAQP + H+VKTKNN V+ + P+PY+LIA
Sbjct:    1 MTNTKGYRRGTRYLFSRKFRHHGPLPSSVYMRVYRRGDIVDIKGDGGVQKGMPHKSYHGRTGRVYNVTPHAVGIIVNKRVGNRIIPKRINVRIEHVKPSKCREDFLRRVKENDVKRRDAKERNVFVQLKRQPAQPRAGHLVKTKNNPVVTVHPLPYELIA 160          
BLAST of 60S ribosomal protein L21 vs. Ensembl Cavefish
Match: rpl21 (ribosomal protein L21 [Source:NCBI gene;Acc:103034585])

HSP 1 Score: 215.312 bits (547), Expect = 6.031e-72
Identity = 99/160 (61.88%), Postives = 127/160 (79.38%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NT+G RRGTRY+F + FRKHG +P ST++ +YK+GD VDIKG G++QKGMPHKCYHG+TGRVYNVTQHA+G+IVNK+V  +I+ K+INVRIEHVK SK R+ FL RVK+N++ K  AK+  T++ LKR PA P  AH V TKNN    L+P+PY+ +A
Sbjct:    1 MTNTRGKRRGTRYMFARPFRKHGPVPLSTYMRIYKKGDIVDIKGTGTIQKGMPHKCYHGKTGRVYNVTQHAVGIIVNKQVKGKILAKRINVRIEHVKHSKSRDSFLQRVKENEKRKMEAKQANTWVELKRQPAAPRPAHFVSTKNNEPQLLEPIPYEFMA 160          
BLAST of 60S ribosomal protein L21 vs. Ensembl Sea Lamprey
Match: rpl21 (ribosomal protein L21 [Source:ZFIN;Acc:ZDB-GENE-030131-8512])

HSP 1 Score: 174.866 bits (442), Expect = 1.174e-56
Identity = 87/162 (53.70%), Postives = 113/162 (69.75%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKH--GTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M  T G RRGTRY+F + FRKH  G +P ST++ +YK+GD VDIKG G+VQ+GMPHKCYHG+TGRV+N+TQHA+GVIVNK+V  +++ K+INVRIEHVK SK R+ FL RVK+N+  K  AK  G ++ LKR      + H V+ K    +     P Q  A
Sbjct:    1 MGKTHGKRRGTRYMFARSFRKHVSGPIPLSTYMKIYKKGDIVDIKGTGTVQQGMPHKCYHGKTGRVFNITQHAVGVIVNKQVKGKVLAKRINVRIEHVKHSKSRDSFLKRVKENELKKDKAKLAGEWVTLKRQYVHVTTGHFVEKKRGQSLPCGFTPRQFAA 162          
BLAST of 60S ribosomal protein L21 vs. Ensembl Yeast
Match: RPL21A (Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication [Source:SGD;Acc:S000000395])

HSP 1 Score: 181.8 bits (460), Expect = 1.507e-59
Identity = 85/157 (54.14%), Postives = 114/157 (72.61%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQ 540
            M  + GYR  TRY+F+++FRKHG +  ST+L VYK GD VDIK +GS+QKGMPHK Y G+TG VYNVT+ ++GVI+NK VGNR + K++N+R+EH+K SKCR++FL RVK N   +  AK  G  + LKR PAQP  + +V T+ N    L P+PY+
Sbjct:    1 MGKSHGYRSRTRYMFQRDFRKHGAVHLSTYLKVYKVGDIVDIKANGSIQKGMPHKFYQGKTGVVYNVTKSSVGVIINKMVGNRYLEKRLNLRVEHIKHSKCRQEFLERVKANAAKRAEAKAQGVAVQLKRQPAQPRESRIVSTEGNVPQTLAPVPYE 157          
BLAST of 60S ribosomal protein L21 vs. Ensembl Yeast
Match: RPL21B (Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication [Source:SGD;Acc:S000006000])

HSP 1 Score: 181.415 bits (459), Expect = 1.895e-59
Identity = 84/157 (53.50%), Postives = 114/157 (72.61%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQ 540
            M  + GYR  TRY+F+++FRKHG +  ST+L +YK GD VDIK +GS+QKGMPHK Y G+TG VYNVT+ ++GVI+NK VGNR + K++N+R+EH+K SKCR++FL RVK N   +  AK  G  + LKR PAQP  + +V T+ N    L P+PY+
Sbjct:    1 MGKSHGYRSRTRYMFQRDFRKHGAVHMSTYLKIYKVGDIVDIKANGSIQKGMPHKFYQGKTGVVYNVTKSSVGVIINKMVGNRYLEKRLNLRVEHIKHSKCRQEFLERVKANAAKRAEAKAQGVAVQLKRQPAQPRESRIVSTEGNVPQTLAPVPYE 157          
BLAST of 60S ribosomal protein L21 vs. Ensembl Nematostella
Match: EDO38982 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBD1])

HSP 1 Score: 198.749 bits (504), Expect = 8.539e-66
Identity = 95/159 (59.75%), Postives = 119/159 (74.84%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLI 546
            M NTKGYRRGTRY+F K+FR  G    ST+L  YK GD VD+K +G+VQKGMPHK YHG+TGRVYNVT+ ALGV+VNK+V  +I+ K+INVR+EHVK S CR+DFL RVK+N+  K  AK  G     KR PAQP +AH V+TK N  + L+P+PY+ +
Sbjct:    1 MTNTKGYRRGTRYMFSKKFRHRGVEHLSTYLKCYKVGDIVDVKANGAVQKGMPHKVYHGKTGRVYNVTKRALGVVVNKQVKGKILAKRINVRVEHVKHSTCRDDFLRRVKENERKKHEAKAKGLIAHCKRQPAQPKTAHFVRTKRNKPVTLQPIPYEFV 159          
BLAST of 60S ribosomal protein L21 vs. Ensembl Medaka
Match: rpl21 (ribosomal protein L21 [Source:NCBI gene;Acc:101161099])

HSP 1 Score: 215.698 bits (548), Expect = 5.314e-72
Identity = 99/160 (61.88%), Postives = 126/160 (78.75%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NT+G RRGTRY+F + FRKHG +P ST++ +YK+GD VDIKG G++QKGMPHKCYHG+TGRVYNVTQHA+G+IVNK+V  +I+ K+INVRIEHVK SK R+ FL RVK N+  K  AK+ GT++ LKR PA P+ AH V TK N    L+P+PY+ +A
Sbjct:    1 MTNTRGKRRGTRYMFSRPFRKHGPVPLSTYMRIYKKGDIVDIKGTGTIQKGMPHKCYHGKTGRVYNVTQHAVGIIVNKQVKGKILAKRINVRIEHVKHSKSRDSFLQRVKANEAKKLEAKQKGTWVELKRQPAPPSEAHFVSTKKNEPQLLEPIPYEFMA 160          
BLAST of 60S ribosomal protein L21 vs. Ensembl Medaka
Match: rpl21 (ribosomal protein L21 [Source:NCBI gene;Acc:101161099])

HSP 1 Score: 215.698 bits (548), Expect = 5.314e-72
Identity = 99/160 (61.88%), Postives = 126/160 (78.75%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            M NT+G RRGTRY+F + FRKHG +P ST++ +YK+GD VDIKG G++QKGMPHKCYHG+TGRVYNVTQHA+G+IVNK+V  +I+ K+INVRIEHVK SK R+ FL RVK N+  K  AK+ GT++ LKR PA P+ AH V TK N    L+P+PY+ +A
Sbjct:    1 MTNTRGKRRGTRYMFSRPFRKHGPVPLSTYMRIYKKGDIVDIKGTGTIQKGMPHKCYHGKTGRVYNVTQHAVGIIVNKQVKGKILAKRINVRIEHVKHSKSRDSFLQRVKANEAKKLEAKQKGTWVELKRQPAPPSEAHFVSTKKNEPQLLEPIPYEFMA 160          
BLAST of 60S ribosomal protein L21 vs. Planmine SMEST
Match: SMESG000040216.1 (SMESG000040216.1)

HSP 1 Score: 328.946 bits (842), Expect = 5.911e-117
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 1
Query:   70 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 549
            MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA
Sbjct:    1 MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQKGMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSKCREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVICLKPMPYQLIA 160          
The following BLAST results are available for this feature:
BLAST of 60S ribosomal protein L21 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RPL211.771e-7161.25ribosomal protein L21 [Source:HGNC Symbol;Acc:HGNC... [more]
RPL211.771e-7161.25ribosomal protein L21 [Source:HGNC Symbol;Acc:HGNC... [more]
RPL211.771e-7161.25ribosomal protein L21 [Source:HGNC Symbol;Acc:HGNC... [more]
RPL211.771e-7161.25ribosomal protein L21 [Source:HGNC Symbol;Acc:HGNC... [more]
RPL216.729e-5766.94ribosomal protein L21 [Source:HGNC Symbol;Acc:HGNC... [more]
back to top
BLAST of 60S ribosomal protein L21 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpl-213.040e-6659.0160S ribosomal protein L21 [Source:UniProtKB/Swiss... [more]
back to top
BLAST of 60S ribosomal protein L21 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
RpL212.845e-6860.63gene:FBgn0032987 transcript:FBtr0085961[more]
RpL212.845e-6860.63gene:FBgn0032987 transcript:FBtr0345541[more]
RpL212.845e-6860.63gene:FBgn0032987 transcript:FBtr0331295[more]
back to top
BLAST of 60S ribosomal protein L21 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
rpl219.997e-6862.50ribosomal protein L21 [Source:ZFIN;Acc:ZDB-GENE-03... [more]
rpl219.997e-6862.50ribosomal protein L21 [Source:ZFIN;Acc:ZDB-GENE-03... [more]
rpl219.997e-6862.50ribosomal protein L21 [Source:ZFIN;Acc:ZDB-GENE-03... [more]
AL929009.31.663e-738.60pep chromosome:GRCz11:24:21603588:21637183:-1 gene... [more]
back to top
BLAST of 60S ribosomal protein L21 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpl214.453e-6459.38ribosomal protein L21 [Source:NCBI gene;Acc:448539... [more]
back to top
BLAST of 60S ribosomal protein L21 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Rpl217.114e-7261.88ribosomal protein L21 [Source:MGI Symbol;Acc:MGI:1... [more]
Rpl217.114e-7261.88ribosomal protein L21 [Source:MGI Symbol;Acc:MGI:1... [more]
Rpl217.114e-7261.88ribosomal protein L21 [Source:MGI Symbol;Acc:MGI:1... [more]
back to top
BLAST of 60S ribosomal protein L21 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P49666|RL21_PIG8.506e-7161.2560S ribosomal protein L21 OS=Sus scrofa OX=9823 GN... [more]
sp|P46778|RL21_HUMAN8.506e-7161.2560S ribosomal protein L21 OS=Homo sapiens OX=9606 ... [more]
sp|A5JSS2|RL21_CAPHI1.519e-7061.2560S ribosomal protein L21 OS=Capra hircus OX=9925 ... [more]
sp|Q6QN05|RL21_CHILA2.929e-7060.6360S ribosomal protein L21 OS=Chinchilla lanigera O... [more]
sp|O09167|RL21_MOUSE1.861e-6961.2560S ribosomal protein L21 OS=Mus musculus OX=10090... [more]
back to top
BLAST of 60S ribosomal protein L21 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4E0RLY11.340e-8375.63Ribosomal protein rpl21 OS=Fasciola hepatica OX=61... [more]
A0A504YDH91.029e-8275.00Ribosomal protein rpl21 OS=Fasciola gigantica OX=4... [more]
A0A183AY616.996e-8274.38Uncharacterized protein OS=Echinostoma caproni OX=... [more]
A0A183MM131.108e-8173.75Uncharacterized protein OS=Schistosoma margrebowie... [more]
A0A183RED53.351e-8173.13Uncharacterized protein OS=Schistosoma rodhaini OX... [more]
back to top
BLAST of 60S ribosomal protein L21 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpl216.031e-7261.88ribosomal protein L21 [Source:NCBI gene;Acc:103034... [more]
back to top
BLAST of 60S ribosomal protein L21 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpl211.174e-5653.70ribosomal protein L21 [Source:ZFIN;Acc:ZDB-GENE-03... [more]
back to top
BLAST of 60S ribosomal protein L21 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
RPL21A1.507e-5954.14Ribosomal 60S subunit protein L21A; homologous to ... [more]
RPL21B1.895e-5953.50Ribosomal 60S subunit protein L21B; homologous to ... [more]
back to top
BLAST of 60S ribosomal protein L21 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO389828.539e-6659.75Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of 60S ribosomal protein L21 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rpl215.314e-7261.88ribosomal protein L21 [Source:NCBI gene;Acc:101161... [more]
rpl215.314e-7261.88ribosomal protein L21 [Source:NCBI gene;Acc:101161... [more]
back to top
BLAST of 60S ribosomal protein L21 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000040216.15.911e-117100.00SMESG000040216.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015534 ID=SMED30015534|Name=60S ribosomal protein L21|organism=Schmidtea mediterranea sexual|type=transcript|length=573bp
ATTAAAATTAAATAAAGGTAAGTTGTTTAAATTTTGTGTTTTAATTAAAA
TAACTAGTTTTAAATAAAAATGGTTAATACTAAAGGTTATCGACGTGGAA
CCAGATATCTTTTCAAAAAAGAATTTCGTAAACACGGAACTTTACCAACT
TCTACATTCTTAACCGTTTACAAAAGAGGAGATCATGTTGATATAAAAGG
TGATGGTTCAGTCCAAAAGGGTATGCCACATAAATGCTATCACGGAAGAA
CTGGTCGAGTCTACAATGTTACTCAACATGCATTGGGTGTTATTGTTAAT
AAACGTGTTGGAAACCGTATTATTCCAAAGAAAATTAATGTGAGAATTGA
ACATGTTAAAAAGTCGAAATGCCGTGAAGATTTTCTTAACAGAGTTAAGA
AAAACGATGAAATAAAAAGACTAGCTAAGGAAACTGGAACTTTCATCCCA
TTGAAGAGAATGCCAGCTCAACCAGCTTCTGCTCATGTTGTTAAAACTAA
AAATAACAGTGTTATTTGTTTGAAACCAATGCCATACCAACTGATAGCCT
AAAAATATATTTTAACTTGGATC
back to top

protein sequence of SMED30015534-orf-1

>SMED30015534-orf-1 ID=SMED30015534-orf-1|Name=SMED30015534-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=161bp
MVNTKGYRRGTRYLFKKEFRKHGTLPTSTFLTVYKRGDHVDIKGDGSVQK
GMPHKCYHGRTGRVYNVTQHALGVIVNKRVGNRIIPKKINVRIEHVKKSK
CREDFLNRVKKNDEIKRLAKETGTFIPLKRMPAQPASAHVVKTKNNSVIC
LKPMPYQLIA*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000018Category 4 cell
PLANA:0000026gut
PLANA:0000039gamma neoblast
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR036948Ribosomal_L21_sf
IPR001147Ribosomal_L21e
IPR018259Ribosomal_L21e_CS
IPR008991Translation_prot_SH3-like_sf
Vocabulary: biological process
TermDefinition
GO:0006412translation
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
Vocabulary: cellular component
TermDefinition
GO:0005622intracellular
GO:0005840ribosome
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001147Ribosomal protein L21ePFAMPF01157Ribosomal_L21ecoord: 1..101
e-value: 6.1E-48
score: 160.8
IPR001147Ribosomal protein L21ePANTHERPTHR2098160S RIBOSOMAL PROTEIN L21coord: 1..159
IPR036948Ribosomal protein L21 superfamilyGENE3DG3DSA:2.30.30.70coord: 1..98
e-value: 9.1E-48
score: 162.3
IPR018259Ribosomal protein L21e, conserved sitePROSITEPS01171RIBOSOMAL_L21Ecoord: 37..62
IPR008991Translation protein SH3-like domain superfamilySUPERFAMILYSSF50104Translation proteins SH3-like domaincoord: 3..100