DNA topoisomerase 2
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30015481 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Match: TOP2A (DNA topoisomerase II alpha [Source:HGNC Symbol;Acc:HGNC:11989]) HSP 1 Score: 479.559 bits (1233), Expect = 7.474e-152 Identity = 250/491 (50.92%), Postives = 331/491 (67.41%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQD-----GKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAE 1506
Q N++ S + +P L DAN AG +S ECTLILTEG SAK+ A+S L RD YGVFPL+GK+LNVR+ S + A+I N++KI+GL Y + YE ++ LK LRYGK+MIMTD D +GSHIKGLLINFIH WP LL+H F+E+F PIVK N + +FYS+ E E+W+ TP+ W++KYYKGLGT TS+EA EYF D KR+ I+F+Y+G +D +I LAFS+ + ++RKEWL ++E + K L L +D Y+T FI ELIL+ N+RSIPS++DGL PG RKVLFTC + + E+ V+QLAGSV ++S+YH E SLM I LAQ+FVGSNN+N+L PIGQFGTRL GGKD A PRY++T L+ L +L+F P D L + DDN + EPEWY PIIPM+LING IG GWS IP F ++++ +++ ++GE + + P Y+NF GTI E
Sbjct: 419 AQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHR-----FLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSSLARLLFPPKDDHTLKFLYDDNQR---VEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREIVNNIRRLMDGEEPLPMLPSYKNFKGTIEE 901
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Match: TOP2B (DNA topoisomerase II beta [Source:HGNC Symbol;Acc:HGNC:11990]) HSP 1 Score: 465.692 bits (1197), Expect = 8.431e-150 Identity = 248/498 (49.80%), Postives = 330/498 (66.27%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLH------LDQDGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q N++ S + +P L DAN AG S ECTLILTEG SAKS A+S L RD YGVFPL+GK+LNVR+ S + A+I N++KI+GL Y + Y+ E LK LRYGK+MIMTD D +GSHIKGLLINFIH WP LLKH F+E+F PIVKA N + SFYSI E ++W+K + +W++KYYKGLGT T++EA EYF D +R+ I FRY G +D +I LAFS+ K ++RKEWL ++E + + LH L K++T FI ELIL+ N+RSIPSL+DG PG RKVLFTC + + E+ V+QLAGSV ++SAYH E++LM I LAQ+FVGSNNIN+L PIGQFGTRL GGKD A PRY++T L+ L +L+F D +L + DDN + EPEWY PIIPM+LING IG GW+ +P + ++++ +++ ++G + + P Y+NF GTI E + ++
Sbjct: 435 AQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHG-----FLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRR-QRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQR---VEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQY 923
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Match: TOP2B (DNA topoisomerase II beta [Source:HGNC Symbol;Acc:HGNC:11990]) HSP 1 Score: 464.922 bits (1195), Expect = 6.144e-146 Identity = 248/498 (49.80%), Postives = 330/498 (66.27%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLH------LDQDGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q N++ S + +P L DAN AG S ECTLILTEG SAKS A+S L RD YGVFPL+GK+LNVR+ S + A+I N++KI+GL Y + Y+ E LK LRYGK+MIMTD D +GSHIKGLLINFIH WP LLKH F+E+F PIVKA N + SFYSI E ++W+K + +W++KYYKGLGT T++EA EYF D +R+ I FRY G +D +I LAFS+ K ++RKEWL ++E + + LH L K++T FI ELIL+ N+RSIPSL+DG PG RKVLFTC + + E+ V+QLAGSV ++SAYH E++LM I LAQ+FVGSNNIN+L PIGQFGTRL GGKD A PRY++T L+ L +L+F D +L + DDN + EPEWY PIIPM+LING IG GW+ +P + ++++ +++ ++G + + P Y+NF GTI E + ++
Sbjct: 435 AQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHG-----FLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDR-RQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQR---VEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQY 923
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Match: TOP2B (DNA topoisomerase II beta [Source:HGNC Symbol;Acc:HGNC:11990]) HSP 1 Score: 465.307 bits (1196), Expect = 6.364e-146 Identity = 248/498 (49.80%), Postives = 330/498 (66.27%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLH------LDQDGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q N++ S + +P L DAN AG S ECTLILTEG SAKS A+S L RD YGVFPL+GK+LNVR+ S + A+I N++KI+GL Y + Y+ E LK LRYGK+MIMTD D +GSHIKGLLINFIH WP LLKH F+E+F PIVKA N + SFYSI E ++W+K + +W++KYYKGLGT T++EA EYF D +R+ I FRY G +D +I LAFS+ K ++RKEWL ++E + + LH L K++T FI ELIL+ N+RSIPSL+DG PG RKVLFTC + + E+ V+QLAGSV ++SAYH E++LM I LAQ+FVGSNNIN+L PIGQFGTRL GGKD A PRY++T L+ L +L+F D +L + DDN + EPEWY PIIPM+LING IG GW+ +P + ++++ +++ ++G + + P Y+NF GTI E + ++
Sbjct: 440 AQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHG-----FLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDR-RQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQR---VEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQY 928
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Match: top-2 (DNA topoisomerase 2 top-2 [Source:UniProtKB/Swiss-Prot;Acc:Q23670]) HSP 1 Score: 471.085 bits (1211), Expect = 2.945e-149 Identity = 234/478 (48.95%), Postives = 330/478 (69.04%), Query Frame = 1
Query: 112 VPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQD-----GKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
+P L DAN AGT +S +CTLILTEG SAK+ A+S L+ RD YGVFPL+GKLLNVR+G+ + + A++ ++KI+GL Y +KYE+++ K LRYGKLM+M D D +GSHIKGL+INFIH +WP L++ N F+E+F PIVKA + SFYS+ E +W+ T + S+++KYYKGLGT TS+EA EYF D R+ I+F+YNG D N++D+AFS+ K ERK+WL K++ K K L ++ +++T + F+ EL+L+ + N+RSIP L+DG PG RKVLF C + E+ V+QLAG+V ++SAYH E+SLM I LAQD+VGSNNIN+L+PIGQFGTRL GGKD A RY++T+L+ +T+ +F D +L + ++N + EPEWY PIIPM+L+NG IG GWS++IP ++P +++ +K+ I GE + PWY+NF G I + +F
Sbjct: 473 IPKLEDANDAGTKNSQQCTLILTEGDSAKTLAVSGLSVVGRDKYGVFPLRGKLLNVREGNMKQIADNAEVNAMIKILGLQYKKKYETEDDFKTLRYGKLMVMADQDQDGSHIKGLVINFIHHFWPSLIQRN-----FVEEFITPIVKATKGKEEVSFYSLPEYSEWRMNTDNWKSYKIKYYKGLGTSTSKEAKEYFLDMVRHRIRFKYNGADDDNAVDMAFSKKKIEERKDWLSKWMREKKDRKQQGLAEEYLYNKDTRFVTFKDFVNRELVLFSNLDNERSIPCLVDGFKPGQRKVLFACFKRADKREVKVAQLAGAVAEISAYHHGEQSLMGTIVNLAQDYVGSNNINLLLPIGQFGTRLQGGKDSASARYIFTQLSPVTRTLFPAHDDNVLRFLYEENQR---IEPEWYCPIIPMVLVNGAQGIGTGWSTNIPNYNPRELVKNIKRLIAGEPQKALAPWYKNFRGKIIQIDPSRF 942
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Match: cin-4 (DNA topoisomerase-like protein cin-4 [Source:UniProtKB/Swiss-Prot;Acc:G5ECQ8]) HSP 1 Score: 417.542 bits (1072), Expect = 6.628e-135 Identity = 217/496 (43.75%), Postives = 316/496 (63.71%), Query Frame = 1
Query: 58 QFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNV--RKGSEFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQDG-----KKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q P R++S +P L DAN AGT +S +CTLILTEG SAK+ A+S L+ RD YGVFP + KLLNV ++ + A++ ++KI+GL Y +KYE+++ K LRYGKL+IM + D +GS KGLL+NF H +WP L K DF+E F PI KA + SFYS+ E +W+ T + S+ +KYY GLGT TS+EA + F D R+ I+F+YNG D ++++AFS+ K R ++L K ++ K+ K L ++ +++T++ F YE++ +H SIP L+DGL PG RKVLF C + E+ V+QLAG+V ++SAYH E+SLM I LAQD+VGS+NIN+L+PIGQFGTRL GGKD A R ++ +L+++T+ +F D +L + ++N + EPEWY PIIPM+L+NG G GWS++IP ++P +++ +K+ I GE + PWY+NF G I + +F
Sbjct: 27 QKCPKRKTSKLKG-----IPKLEDANDAGTKNSQQCTLILTEGDSAKTLAVSGLSVVGRDKYGVFPFRRKLLNVCDLNVNQIADSAEVNAIIKILGLQYTKKYETEDDFKTLRYGKLLIMANHDSDGSQFKGLLLNFFHRFWPALFKR-----DFVEDFITPIAKATEGKEEVSFYSLPEYSEWRMNTDNWKSYTIKYYNGLGTLTSKEAKKCFSDMVRHRIRFKYNGADDDKAVNMAFSKKKIEARTDYLMKLMQDKNQRKQQGLAEECLYNKETRFVTLKDFFNYEIVCSWNLH---SIPCLVDGLKPGQRKVLFACFKRANKREVKVAQLAGAVAEISAYHHGEQSLMGTIVNLAQDYVGSHNINLLLPIGQFGTRLQGGKDSASARSIFAQLSQVTRTLFPAHDDNVLRFLYEENQR---IEPEWYCPIIPMVLVNGAQGTGTGWSTNIPNYNPRELVKNIKRLIAGEPQKALAPWYKNFRGKIIQIDPSRF 506
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Match: R05D3.12 (Putative DNA topoisomerase 2, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:P34534]) HSP 1 Score: 372.474 bits (955), Expect = 8.271e-115 Identity = 207/492 (42.07%), Postives = 308/492 (62.60%), Query Frame = 1
Query: 64 MPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGSEFR--NRAKIENLVKIIGLDYNQKYESKELLK-GLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQDGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCL-EMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENT-VKIHPWYRNFTGTIAEKSEKKF 1524
+P + S +S + +V + + A GTA+ +CTLILTEG SAK+ A++ L R+ YGVFPL+GKLLNV + R +I NL++I+GL + +S + + LRYG+L+I+ D D +GSHIKGL++NFIH +WP L+ + FI+ F P++KA +KV SF+S+ E KW + + W++KYYKGLGT TS EA EYF D + + F+Y G D ++I +AF DK++ERKEW+ + + + DGK I+ ++F+ +L+ +G++ KRSIPSL+DGL P RK+L+T L MD E+ VSQLAG+V +YH EESL+ I ++ Q F GS+N+ +L PIGQFGTR +GG D A RY++T L T+L+F D +L + +++ + EP W PI+P+ILING IG GWS+ I +PID+ID ++++I+ +T +I P+Y F G + + KF
Sbjct: 444 LPGKRVSVSSVRDIVKLEDAEWAGITGTAE--KCTLILTEGDSAKALALAGLEVLGRETYGVFPLKGKLLNVSNLDDARASKNEEISNLLRILGLKFE---DSNSITRESLRYGRLLILADQDEDGSHIKGLIVNFIHKFWPSLVHTD----GFIQSFRTPLLKAKKGDKVRSFFSMNEYRKWA-DVEEGGKWKIKYYKGLGTSTSNEAREYFSDLDHHTVNFKYTGTTDDDAIRMAFDRDKSDERKEWIRRS------ENEITNEDDGKTEISYQEFVDGQLMQFGMVDLKRSIPSLIDGLKPSQRKILWTLLNNMDESTEIKVSQLAGAVAHRQSYHHGEESLVRTIIRMGQTFCGSSNLPLLQPIGQFGTRHEGGNDAASARYIFTALAPTTRLLFPQADDDLLQKNVEEG---MVVEPTWLCPIVPLILINGTEGIGTGWSTKIANRNPIDIIDMIRRKIDSISTEYEIPPFYEEFRGKLEVVTPTKF 916
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Match: R05D3.12 (Putative DNA topoisomerase 2, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:P34534]) HSP 1 Score: 218.394 bits (555), Expect = 3.405e-61 Identity = 119/295 (40.34%), Postives = 182/295 (61.69%), Query Frame = 1
Query: 64 MPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGSEFR--NRAKIENLVKIIGLDYNQKYESKELL--KGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQDGKKYITIRKFIKYELILYGIMHNKRS 936
+P + S +S + +V + + A GTA+ +CTLILTEG SAK+ A++ L R+ YGVFPL+GKLLNV + R +I NL++I+GL K+E + + LRYG+L+I+ D D +GSHIKGL++NFIH +WP L+ + FI+ F P++KA +KV SF+S+ E KW + + W++KYYKGLGT TS EA EYF D + + F+Y G D ++I +AF DK++ERKEW+ + + + DGK I+ ++F+ +L+ +G++ KR+
Sbjct: 444 LPGKRVSVSSVRDIVKLEDAEWAGITGTAE--KCTLILTEGDSAKALALAGLEVLGRETYGVFPLKGKLLNVSNLDDARASKNEEISNLLRILGL----KFEDSNSITRESLRYGRLLILADQDEDGSHIKGLIVNFIHKFWPSLVHTDG----FIQSFRTPLLKAKKGDKVRSFFSMNEYRKWA-DVEEGGKWKIKYYKGLGTSTSNEAREYFSDLDHHTVNFKYTGTTDDDAIRMAFDRDKSDERKEWIRRS------ENEITNEDDGKTEISYQEFVDGQLMQFGMVDLKRT 721
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Match: R05D3.12 (Putative DNA topoisomerase 2, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:P34534]) HSP 1 Score: 218.394 bits (555), Expect = 3.405e-61 Identity = 119/295 (40.34%), Postives = 182/295 (61.69%), Query Frame = 1
Query: 64 MPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGSEFR--NRAKIENLVKIIGLDYNQKYESKELL--KGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQDGKKYITIRKFIKYELILYGIMHNKRS 936
+P + S +S + +V + + A GTA+ +CTLILTEG SAK+ A++ L R+ YGVFPL+GKLLNV + R +I NL++I+GL K+E + + LRYG+L+I+ D D +GSHIKGL++NFIH +WP L+ + FI+ F P++KA +KV SF+S+ E KW + + W++KYYKGLGT TS EA EYF D + + F+Y G D ++I +AF DK++ERKEW+ + + + DGK I+ ++F+ +L+ +G++ KR+
Sbjct: 444 LPGKRVSVSSVRDIVKLEDAEWAGITGTAE--KCTLILTEGDSAKALALAGLEVLGRETYGVFPLKGKLLNVSNLDDARASKNEEISNLLRILGL----KFEDSNSITRESLRYGRLLILADQDEDGSHIKGLIVNFIHKFWPSLVHTDG----FIQSFRTPLLKAKKGDKVRSFFSMNEYRKWA-DVEEGGKWKIKYYKGLGTSTSNEAREYFSDLDHHTVNFKYTGTTDDDAIRMAFDRDKSDERKEWIRRS------ENEITNEDDGKTEISYQEFVDGQLMQFGMVDLKRT 721
BLAST of DNA topoisomerase 2 vs. Ensembl Fly
Match: Top2 (gene:FBgn0284220 transcript:FBtr0332320) HSP 1 Score: 458.759 bits (1179), Expect = 4.670e-145 Identity = 244/481 (50.73%), Postives = 324/481 (67.36%), Query Frame = 1
Query: 103 VVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQ-----DGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
+ +P L DAN AG +S +CTLILTEG SAKS A+S L RD YGVFPL+GKLLNVR+ + + A+I NL KIIGL Y +KY +++ LK LRYGK+MIMTD D +GSHIKGLLINFIH WPELL+ F+E+F PIVKA N+ SFYS+ E E+W+ +T + ++ +KYYKGLGT TS+EA EYF+D R+ I F+Y+G D SI +AFS+ RK WL +++ K L L + G K IT FI EL+L+ N+RSIPSL+DGL PG RKV+FTC + + E+ V+QL+GSV ++SAYH E SL I LAQ+FVG+NNIN+L P GQFGTRL GGKDCA RY++T ++ LT+LI+HP D +L+ +DD K EP WY PIIPM+L+NG IG GWS+ I ++P +++ L++ ING+ +HPWY+NF G + S+ ++
Sbjct: 415 IKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGVIGRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHTNWPELLR-----LPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANHHTYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMDEVKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFKRNDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDCASARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQK---IEPLWYLPIIPMVLVNGAEGIGTGWSTKISNYNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRY 887
BLAST of DNA topoisomerase 2 vs. Ensembl Fly
Match: Top2 (gene:FBgn0284220 transcript:FBtr0081287) HSP 1 Score: 458.759 bits (1179), Expect = 4.670e-145 Identity = 244/481 (50.73%), Postives = 324/481 (67.36%), Query Frame = 1
Query: 103 VVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQ-----DGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
+ +P L DAN AG +S +CTLILTEG SAKS A+S L RD YGVFPL+GKLLNVR+ + + A+I NL KIIGL Y +KY +++ LK LRYGK+MIMTD D +GSHIKGLLINFIH WPELL+ F+E+F PIVKA N+ SFYS+ E E+W+ +T + ++ +KYYKGLGT TS+EA EYF+D R+ I F+Y+G D SI +AFS+ RK WL +++ K L L + G K IT FI EL+L+ N+RSIPSL+DGL PG RKV+FTC + + E+ V+QL+GSV ++SAYH E SL I LAQ+FVG+NNIN+L P GQFGTRL GGKDCA RY++T ++ LT+LI+HP D +L+ +DD K EP WY PIIPM+L+NG IG GWS+ I ++P +++ L++ ING+ +HPWY+NF G + S+ ++
Sbjct: 415 IKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGVIGRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHTNWPELLR-----LPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANHHTYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMDEVKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFKRNDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDCASARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQK---IEPLWYLPIIPMVLVNGAEGIGTGWSTKISNYNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRY 887
BLAST of DNA topoisomerase 2 vs. Ensembl Zebrafish
Match: top2b (DNA topoisomerase II beta [Source:ZFIN;Acc:ZDB-GENE-041008-136]) HSP 1 Score: 474.167 bits (1219), Expect = 1.440e-149 Identity = 247/497 (49.70%), Postives = 334/497 (67.20%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQD-----GKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q N++ S + +P L DAN AG S ECTLILTEG SAKS A+S L RD YGVFPL+GK+LNVR+ + + A+I N++KI+GL Y + Y+ E LK LRYG++MIMTD D +GSHIKGLLINF H WP LLKH F+E+F PIVKA N + SFYSI E E+W+K T +W +KYYKGLGT TS+EA EYF D +R+ I F+YNG +D +I LAFS+ KT++RKEWL ++E + + L + ++++ FI ELIL+ N+RSIPSL+DGL PG RKVLFTC++ + E+ V+QLAGSV ++SAYH E++LM I LAQ+FVGSNN+NIL P+GQFGTR++GGKD A PRY++T L+ L KL+F D ++L + DDN K EPEWY PIIPM+L+NG IG GW+ IP + P ++++ + + +N ++ + + P Y+NF GTI E + ++
Sbjct: 439 AQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSSECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREATHKQIMENAEINNIIKILGLQYKKSYDDPESLKSLRYGRIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHT-----FLEEFITPIVKASKNKQEVSFYSIPEFEEWKKHTESYKTWHIKYYKGLGTSTSKEAKEYFSDMERHRIMFKYNGTEDDAAITLAFSKKKTDDRKEWLTNFMEDRRQRRMHGLPEQYLYGTSTRHLSYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCVKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVNILQPLGQFGTRINGGKDAASPRYIFTMLSPLAKLLFPAVDSSLLKFLYDDNQK---VEPEWYIPIIPMVLVNGAEGIGTGWACKIPNYDPREIVNNINRMLNHQDPLPMLPSYKNFKGTIHELGQNQY 927
BLAST of DNA topoisomerase 2 vs. Ensembl Zebrafish
Match: top2a (DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GENE-030131-2453]) HSP 1 Score: 448.743 bits (1153), Expect = 2.276e-140 Identity = 241/499 (48.30%), Postives = 323/499 (64.73%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQD-----GKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKFNN 1530
Q N++ S + VP L DAN AG +S CTLILTEG SAK+ A+S L RD YGVFPL+GK+LNVR+ S + A+I N++KI+GL Y + Y E LK LRYGKLMIMTD D +GSHIKGLLINFIH WP LL+HN F+E+F PI+KA N + SFYSI E +W+++ SW++KYYKGLGT TS+EA EYF D R+ I F+Y+G D +I LAFS+ ERKEWL ++ + + +L ++ K ++ FI EL+L+ N+RSIP ++DGL PG RKV+F C + + E+ V+QLAGSV ++SAYH E SLM I LAQ+FVGSNN+N+L P+GQFGTRL GGKD A PRY++T L+ L +L+F D +L DDN + EPEWY PIIP++L NG IG GW+S IP + ++I+ + + +NGE + + P Y+ F GTI E ++ ++ N
Sbjct: 418 AQAQLNKKCSAVKHTKIKGVPKLDDANDAGGKNSNSCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKMLNVREASHKQIMENAEINNIIKILGLQYKKNYSDPESLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHN-----FLEEFITPIIKASNKKQEISFYSIPEFTEWKEKQSSIKSWKIKYYKGLGTSTSKEAKEYFSDMTRHRIPFKYSGPADDEAITLAFSKKMIEERKEWLTSFMINRRQRREHNLPEEYLYGQETKSLSYHDFINKELVLFSNSDNERSIPCMVDGLKPGQRKVVFCCFKRNDKREVKVAQLAGSVAEMSAYHHGEVSLMMTIVGLAQNFVGSNNLNLLQPLGQFGTRLHGGKDSASPRYIFTMLSTLARLLFPVVDDNLLKYNYDDN---LRVEPEWYMPIIPLVLANGGEGIGTGWASRIPNYDVREIINNIHRMLNGEEPLPMLPSYKGFKGTIDELAKNQYVN 908
BLAST of DNA topoisomerase 2 vs. Ensembl Zebrafish
Match: top2a (DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GENE-030131-2453]) HSP 1 Score: 134.42 bits (337), Expect = 3.124e-32 Identity = 72/150 (48.00%), Postives = 100/150 (66.67%), Query Frame = 1
Query: 1081 SLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKFNN 1530
SLM I LAQ+FVGSNN+N+L P+GQFGTRL GGKD A PRY++T L+ L +L+F D +L DDN + EPEWY PIIP++L NG IG GW+S IP + ++I+ + + +NGE + + P Y+ F GTI E ++ ++ N
Sbjct: 2 SLMMTIVGLAQNFVGSNNLNLLQPLGQFGTRLHGGKDSASPRYIFTMLSTLARLLFPVVDDNLLKYNYDDN---LRVEPEWYMPIIPLVLANGGEGIGTGWASRIPNYDVREIINNIHRMLNGEEPLPMLPSYKGFKGTIDELAKNQYVN 148
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Match: TOP2B (DNA topoisomerase II beta [Source:NCBI gene;Acc:779457]) HSP 1 Score: 460.685 bits (1184), Expect = 2.368e-147 Identity = 246/498 (49.40%), Postives = 330/498 (66.27%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLH------LDQDGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q N++ S + +P L DAN AG S +CTLILTEG SAKS A+S L RD YGVFPL+GK+LNVR+ S + A+I N++KI+GL Y + Y+ E LK LRYGK+MIMTD D +GSHIKGLLINF H WP LLKH+ F+E+F PIVKA N + SFYSI E E+W+K+T + W++KYYKGLGT TS+EA EYF D R+ I FRY G +D +I LAFS+ K ++RKEWL ++E + + LH L K++T FI ELIL+ N+RSIPSL+DGL PG RKVLFTC + + E+ V+QLAGSV ++SAYH E +LM I LAQ+FVGSNN+++L PIGQFGTRL GGKD A PRY++T L+ L +L++ D +L + DDN + EPEWY PIIP++L+NG IG GW+ +P + ++++ +K+ ++G + + P Y+NF GTI E + ++
Sbjct: 392 AQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDNESLKTLRYGKIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHS-----FLEEFITPIVKATKNKQELSFYSIPEFEEWKKQTENQKLWKIKYYKGLGTSTSKEAKEYFADMDRHRIIFRYAGPEDDAAITLAFSKKKVDDRKEWLTNFMEDRR-QRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEVTLMMTIVNLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLYPSVDDNLLKFLYDDNQR---VEPEWYIPIIPLVLVNGAEGIGTGWACKLPNYDTREIVNNVKRMLDGLDPYPLLPSYKNFKGTIQELGQNQY 880
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Match: TOP2B (DNA topoisomerase II beta [Source:NCBI gene;Acc:779457]) HSP 1 Score: 464.537 bits (1194), Expect = 2.707e-147 Identity = 246/498 (49.40%), Postives = 331/498 (66.47%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLH------LDQDGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q N++ S + +P L DAN AG S +CTLILTEG SAKS A+S L RD YGVFPL+GK+LNVR+ S + A+I N++KI+GL Y + Y+ E LK LRYGK+MIMTD D +GSHIKGLLINF H WP LLKH+ F+E+F PIVKA N + SFYSI E E+W+K+T + W++KYYKGLGT TS+EA EYF D R+ I FRY G +D +I LAFS+ K ++RKEWL ++E + + LH L K++T FI ELIL+ N+RSIPSL+DGL PG RKVLFTC + + E+ V+QLAGSV ++SAYH E++LM I LAQ+FVGSNN+++L PIGQFGTRL GGKD A PRY++T L+ L +L++ D +L + DDN + EPEWY PIIP++L+NG IG GW+ +P + ++++ +K+ ++G + + P Y+NF GTI E + ++
Sbjct: 402 AQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDNESLKTLRYGKIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHS-----FLEEFITPIVKATKNKQELSFYSIPEFEEWKKQTENQKLWKIKYYKGLGTSTSKEAKEYFADMDRHRIIFRYAGPEDDAAITLAFSKKKVDDRKEWLTNFMEDRR-QRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLYPSVDDNLLKFLYDDNQR---VEPEWYIPIIPLVLVNGAEGIGTGWACKLPNYDTREIVNNVKRMLDGLDPYPLLPSYKNFKGTIQELGQNQY 890
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Match: TOP2B (DNA topoisomerase II beta [Source:NCBI gene;Acc:779457]) HSP 1 Score: 463.766 bits (1192), Expect = 8.335e-147 Identity = 246/498 (49.40%), Postives = 331/498 (66.47%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLH------LDQDGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q N++ S + +P L DAN AG S +CTLILTEG SAKS A+S L RD YGVFPL+GK+LNVR+ S + A+I N++KI+GL Y + Y+ E LK LRYGK+MIMTD D +GSHIKGLLINF H WP LLKH+ F+E+F PIVKA N + SFYSI E E+W+K+T + W++KYYKGLGT TS+EA EYF D R+ I FRY G +D +I LAFS+ K ++RKEWL ++E + + LH L K++T FI ELIL+ N+RSIPSL+DGL PG RKVLFTC + + E+ V+QLAGSV ++SAYH E++LM I LAQ+FVGSNN+++L PIGQFGTRL GGKD A PRY++T L+ L +L++ D +L + DDN + EPEWY PIIP++L+NG IG GW+ +P + ++++ +K+ ++G + + P Y+NF GTI E + ++
Sbjct: 441 AQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDNESLKTLRYGKIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHS-----FLEEFITPIVKATKNKQELSFYSIPEFEEWKKQTENQKLWKIKYYKGLGTSTSKEAKEYFADMDRHRIIFRYAGPEDDAAITLAFSKKKVDDRKEWLTNFMEDRR-QRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLYPSVDDNLLKFLYDDNQR---VEPEWYIPIIPLVLVNGAEGIGTGWACKLPNYDTREIVNNVKRMLDGLDPYPLLPSYKNFKGTIQELGQNQY 929
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Match: TOP2B (DNA topoisomerase II beta [Source:NCBI gene;Acc:779457]) HSP 1 Score: 461.84 bits (1187), Expect = 1.183e-146 Identity = 246/498 (49.40%), Postives = 330/498 (66.27%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLH------LDQDGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q N++ S + +P L DAN AG S +CTLILTEG SAKS A+S L RD YGVFPL+GK+LNVR+ S + A+I N++KI+GL Y + Y+ E LK LRYGK+MIMTD D +GSHIKGLLINF H WP LLKH+ F+E+F PIVKA N + SFYSI E E+W+K+T + W++KYYKGLGT TS+EA EYF D R+ I FRY G +D +I LAFS+ K ++RKEWL ++E + + LH L K++T FI ELIL+ N+RSIPSL+DGL PG RKVLFTC + + E+ V+QLAGSV ++SAYH E +LM I LAQ+FVGSNN+++L PIGQFGTRL GGKD A PRY++T L+ L +L++ D +L + DDN + EPEWY PIIP++L+NG IG GW+ +P + ++++ +K+ ++G + + P Y+NF GTI E + ++
Sbjct: 383 AQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDNESLKTLRYGKIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHS-----FLEEFITPIVKATKNKQELSFYSIPEFEEWKKQTENQKLWKIKYYKGLGTSTSKEAKEYFADMDRHRIIFRYAGPEDDAAITLAFSKKKVDDRKEWLTNFMEDRR-QRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEVTLMMTIVNLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLYPSVDDNLLKFLYDDNQR---VEPEWYIPIIPLVLVNGAEGIGTGWACKLPNYDTREIVNNVKRMLDGLDPYPLLPSYKNFKGTIQELGQNQY 871
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Match: TOP2A (DNA topoisomerase II alpha [Source:NCBI gene;Acc:100038293]) HSP 1 Score: 459.914 bits (1182), Expect = 2.181e-146 Identity = 246/492 (50.00%), Postives = 322/492 (65.45%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLH------LDQDGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAE 1506
Q N++ S + +P L DAN AG+ +S +CTLILTEG SAK+ A+S L RD YGVFPL+GKLLNVR+ S + A+I N++KI+GL Y + Y+ E LK LRYGK+MIMTD D +GSHIKGLLINFIH WP LLKH F+E+F PI+K + + SFYSI E E+W+ T +W++KYYKGLGT TS+EA EYF D +R+ I F+Y G D +I LAFS K ++RKEWL +++ + + LH L KY+T FI ELIL+ N+RSIPSL+DGL PG RKVLFTC + + E+ V+QLAGSV ++SAYH E SLM I LAQ+FVGSNN+++L PIGQFGTRL GGKD A PRY++T L+ L +L+F D ++L M DDN K EPEWY PIIP +L+NG IG GWS IP + +++ + + ++G + + P Y+NF G I E
Sbjct: 416 AQTQLNKKCSSVKHTKIKGIPKLDDANDAGSRNSLDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKLLNVREASHKQIMENAEINNIIKIVGLQYKKNYDDNESLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHR-----FLEEFITPIIKVTKSKQELSFYSIPEFEEWKNNTDSHKTWKIKYYKGLGTSTSKEAKEYFSDMERHRIPFKYEGPIDDAAITLAFSRKKVDDRKEWLTNFMQDRR-ERRLHGLPEEYLYGKSTKYLTFNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGETSLMMTIINLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAMDDSVLKFMYDDNQK---VEPEWYIPIIPTVLVNGAEGIGTGWSCKIPNYDTREIVSNIGRLLDGLEPLPMLPSYKNFKGLIQE 898
BLAST of DNA topoisomerase 2 vs. Ensembl Mouse
Match: Top2a (topoisomerase (DNA) II alpha [Source:MGI Symbol;Acc:MGI:98790]) HSP 1 Score: 482.641 bits (1241), Expect = 3.376e-153 Identity = 251/497 (50.50%), Postives = 335/497 (67.40%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQD-----GKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q N++ S + +P L DAN AG+ +S ECTLILTEG SAK+ A+S L RD YGVFPL+GK+LNVR+ S + A+I N++KI+GL Y + YE ++ LK LRYGK+MIMTD D +GSHIKGLLINFIH WP LL+H F+E+F PIVK N + +FYS+ E E+W+ TP+ W++KYYKGLGT TS+EA EYF D KR+ I+F+Y+G +D +I LAFS+ + ++RKEWL ++E + K L L +D Y+T FI ELIL+ N+RSIPS++DGL PG RKVLFTC + + E+ V+QLAGSV ++S+YH E SLM I LAQ+FVGSNN+N+L PIGQFGTRL GGKD A PRY++T L+ L +L+F P D L + DDN + EPEWY PIIPM+LING IG GWS IP F +V++ +++ ++GE + + P Y+NF GTI E + ++
Sbjct: 418 AQIQLNKKCSAVKHTKIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHR-----FLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQSTSYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQR---VEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQY 906
BLAST of DNA topoisomerase 2 vs. Ensembl Mouse
Match: Top2b (topoisomerase (DNA) II beta [Source:MGI Symbol;Acc:MGI:98791]) HSP 1 Score: 465.307 bits (1196), Expect = 3.628e-146 Identity = 248/498 (49.80%), Postives = 329/498 (66.06%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLH------LDQDGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q N++ S + +P L DAN AG S ECTLILTEG SAKS A+S L RD YGVFPL+GK+LNVR+ S + A+I N++KI+GL Y + Y+ E LK LRYGK+MIMTD D +GSHIKGLLINFIH WP LLKH F+E+F PIVKA N + SFYSI E ++W+K + +W++KYYKGLGT T++EA EYF D +R+ I FRY G +D +I LAFS+ K ++RKEWL ++E + + LH L K++T FI ELIL+ N+RSIPSL+DG PG RKVLFTC + + E+ V+QLAGSV ++SAYH E++LM I LAQ+FVGSNNIN+L PIGQFGTRL GGKD A PRY++T L+ L +L+F D +L + DDN + EPEWY PIIPM+LING IG GW+ +P + ++++ +++ + G + + P Y+NF GTI E + ++
Sbjct: 428 AQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHG-----FLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDR-RQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSSLARLLFPAVDDNLLKFLYDDNQR---VEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQELGQNQY 916
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Match: sp|Q01320|TOP2A_MOUSE (DNA topoisomerase 2-alpha OS=Mus musculus OX=10090 GN=Top2a PE=1 SV=2) HSP 1 Score: 482.641 bits (1241), Expect = 2.362e-152 Identity = 251/497 (50.50%), Postives = 335/497 (67.40%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQD-----GKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q N++ S + +P L DAN AG+ +S ECTLILTEG SAK+ A+S L RD YGVFPL+GK+LNVR+ S + A+I N++KI+GL Y + YE ++ LK LRYGK+MIMTD D +GSHIKGLLINFIH WP LL+H F+E+F PIVK N + +FYS+ E E+W+ TP+ W++KYYKGLGT TS+EA EYF D KR+ I+F+Y+G +D +I LAFS+ + ++RKEWL ++E + K L L +D Y+T FI ELIL+ N+RSIPS++DGL PG RKVLFTC + + E+ V+QLAGSV ++S+YH E SLM I LAQ+FVGSNN+N+L PIGQFGTRL GGKD A PRY++T L+ L +L+F P D L + DDN + EPEWY PIIPM+LING IG GWS IP F +V++ +++ ++GE + + P Y+NF GTI E + ++
Sbjct: 418 AQIQLNKKCSAVKHTKIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHR-----FLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQSTSYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQR---VEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQY 906
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Match: sp|P41515|TOP2A_CRIGR (DNA topoisomerase 2-alpha OS=Cricetulus griseus OX=10029 GN=TOP2A PE=1 SV=1) HSP 1 Score: 481.1 bits (1237), Expect = 7.775e-152 Identity = 252/497 (50.70%), Postives = 336/497 (67.61%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQD-----GKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q N++ S + +P L DAN AG+ +S ECTLILTEG SAK+ A+S L RD YGVFPL+GK+LNVR+ S + A+I N++KI+GL Y + YE ++ LK LRYGK+MIMTD D +GSHIKGLLINFIH WP LL+H F+E+F PIVK N + +FYS+ E E+W+ TP+ W++KYYKGLGT TS+EA EYF D KR+ I+F+Y+G +D +I LAFS+ + ++RKEWL ++E + K L L +D Y+T FI ELIL+ N+RSIPS++DGL PG RKVLFTC + + E+ V+QLAGSV ++S+YH E SLM I LAQ+FVGSNN+N+L PIGQFGTRL GGKD A PRY++T L+ LT+L+F P D L + DDN + EPEWY PIIPM+LING IG GWS IP F +V++ +++ ++GE + + P Y+NF GTI E + ++
Sbjct: 418 AQIQLNKKCSAVKHNRIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHR-----FLEEFITPIVKVSKNKQELAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTHFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLTRLLFPPKDDHTLKFLYDDNQR---VEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDIREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQY 906
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Match: sp|P11388|TOP2A_HUMAN (DNA topoisomerase 2-alpha OS=Homo sapiens OX=9606 GN=TOP2A PE=1 SV=3) HSP 1 Score: 479.559 bits (1233), Expect = 3.589e-151 Identity = 250/491 (50.92%), Postives = 331/491 (67.41%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQD-----GKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAE 1506
Q N++ S + +P L DAN AG +S ECTLILTEG SAK+ A+S L RD YGVFPL+GK+LNVR+ S + A+I N++KI+GL Y + YE ++ LK LRYGK+MIMTD D +GSHIKGLLINFIH WP LL+H F+E+F PIVK N + +FYS+ E E+W+ TP+ W++KYYKGLGT TS+EA EYF D KR+ I+F+Y+G +D +I LAFS+ + ++RKEWL ++E + K L L +D Y+T FI ELIL+ N+RSIPS++DGL PG RKVLFTC + + E+ V+QLAGSV ++S+YH E SLM I LAQ+FVGSNN+N+L PIGQFGTRL GGKD A PRY++T L+ L +L+F P D L + DDN + EPEWY PIIPM+LING IG GWS IP F ++++ +++ ++GE + + P Y+NF GTI E
Sbjct: 419 AQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHR-----FLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSSLARLLFPPKDDHTLKFLYDDNQR---VEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREIVNNIRRLMDGEEPLPMLPSYKNFKGTIEE 901
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Match: sp|O46374|TOP2A_PIG (DNA topoisomerase 2-alpha OS=Sus scrofa OX=9823 GN=TOP2A PE=2 SV=1) HSP 1 Score: 477.633 bits (1228), Expect = 1.844e-150 Identity = 251/491 (51.12%), Postives = 330/491 (67.21%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQD-----GKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAE 1506
Q N++ S + +P L DAN AG +S ECTLILTEG SAK+ A+S L RD YGVFPL+GK+LNVR+ S + A+I N++KI+GL Y + YE ++ LK LRYGK+MIMTD D +GSHIKGLLINFIH WP LL+H F+E+F PIVK N + +FYS+ E E+W+ TP+ W++KYYKGLGT TS+EA EYF D KR+ I+F+Y+G +D +I LAFS+ + ++RKEWL ++E + K L L +D Y+T FI ELIL+ N+RSIPS++DGL PG RKVLFTC + + E+ V+QLAGSV ++S+YH E SLM I LAQ+FVGSNN+N+L PIGQFGTRL GGKD A PRY++T L+ L +L+F P D L + DDN + EPEWY PIIPM+LING IG GWS IP F +V++ ++ ++GE + + P Y+NF GTI E
Sbjct: 419 AQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHR-----FLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTHFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLKFLYDDNQR---VEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRLLMDGEEPLPMLPSYKNFKGTIEE 901
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Match: sp|P41516|TOP2A_RAT (DNA topoisomerase 2-alpha OS=Rattus norvegicus OX=10116 GN=Top2a PE=1 SV=1) HSP 1 Score: 472.241 bits (1214), Expect = 1.872e-148 Identity = 247/497 (49.70%), Postives = 333/497 (67.00%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQD-----GKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q N++ S + +P L DAN AG+ +S ECTLILTEG SAK+ A+S L RD YGVFPL+GK+LNVR+ S + A+I N++KI+GL Y + YE ++ LK LRYGK+MIMTD D +GSHIKGLLINFIH WP LL+H F+E+F PIVK N + +FYS+ E E+W+ P+ W++KYYKGLGT TS+EA EYF + KR+ I+F+Y+G +D +I LAFS+ + ++RKEWL ++E + K L L +D Y+T FI ELIL+ N+RSIPS++DGL PG RKVLFTC + + E+ V+QLAGSV ++S+YH E SLM I LAQ+FVGSNN+N+L PIGQFGTRL GGKD A PRY++T L+ L +L+F D L + DDN + EPEWY PIIPM+LING IG GWS IP F +V++ +++ ++GE + + P Y+N+ GTI E + ++
Sbjct: 417 AQIQLNKKCSAVKHNRIKGIPKLDDANDAGSRNSAECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHR-----FLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSTNPNHKKWKVKYYKGLGTSTSKEAKEYFANMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTMYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPSKDDHTLRFLYDDNQR---VEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNYKGTIEELASNQY 905
BLAST of DNA topoisomerase 2 vs. TrEMBL
Match: A0A5K3EPX2 (DNA topoisomerase 2 OS=Mesocestoides corti OX=53468 PE=4 SV=1) HSP 1 Score: 491.115 bits (1263), Expect = 1.157e-159 Identity = 256/490 (52.24%), Postives = 337/490 (68.78%), Query Frame = 1
Query: 112 VPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKG--SEFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQD-----GKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKFNNMTLFDECGVI 1560
+P L DAN AGT +S CTLILTEG SAKS A++ L RD YGVFPL+GKLLNVR+ + A+I NL+KI+GL Y KYE++E L LRYGK+MIMTD D +GSHIKGL+I+FIH WP+LL HN F+EQF PIVK F + +FYSI E E+WQK TP+ +WR+KYYKGLGT +S+EA EYF D R+ I+FRY+G +D + I LAF+++K NERK+WL ++E K L L +D G +T FI EL+L+ M N+RSIPSL+DGL PG RKV+FTCL+ + V+E+ V+QL G+V ++S+YH E SLM I LAQDFVGSNN+N+LMP GQFGTRL GGKD A RY++T L+ LT+ IFH +D AIL+ + +DN K EP WY P+IPM+LING IG GWS+ +P + +VI +++ ++G + + + P YR + G I E E ++ LF E V+
Sbjct: 241 IPKLDDANDAGTKNSQHCTLILTEGDSAKSLAVAGLGVVGRDRYGVFPLRGKLLNVREAPTRQIMENAEINNLIKILGLQYKNKYETQESLANLRYGKIMIMTDQDQDGSHIKGLIISFIHHNWPKLLHHN-----FLEQFITPIVKIFKGKQEIAFYSIPEFEEWQKATPNWHTWRVKYYKGLGTSSSKEAKEYFSDMTRHRIRFRYSGPEDDSGIVLAFNKNKINERKQWLTSWMEEKRRRTELGLPEDYLYGAGTHTVTYHDFIHKELVLFSNMDNERSIPSLVDGLKPGQRKVMFTCLKRNQVKEIKVAQLCGAVSEMSSYHHGEMSLMGTIVGLAQDFVGSNNLNLLMPYGQFGTRLSGGKDSASARYIFTALSPLTRKIFHENDDAILHYLFEDNQK---IEPSWYIPVIPMVLINGAEGIGTGWSTKVPNYDTAEVIANIRRMLDGVDPLPMLPSYRGYRGKIVEVGENRY---ILFGEIAVL 719
BLAST of DNA topoisomerase 2 vs. TrEMBL
Match: A0A183JF90 (Toprim domain-containing protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS1356 PE=4 SV=1) HSP 1 Score: 488.804 bits (1257), Expect = 1.474e-158 Identity = 256/478 (53.56%), Postives = 337/478 (70.50%), Query Frame = 1
Query: 112 VPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQD---GK--KYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
+P L DAN AGT +S +CTLILTEG SAK+ A++ L RD YGVFPL+GKLLNVR+ S + A+I L+KI+GL Y KYES + LK LRYGK+MIMTD D +GSHIKGL+INF+H WP LLKHN +E +F PIVK F + SFYS+ E E+WQK TP+ +WR+KYYKGLGT TS+EA EYF D R+ I+FRY+G +D SI LAF + K +RK WL + + + + L L + GK + IT F+ EL+L+ + N+RSIPS++DGL PG RKVLFTCL+ +L+ E+ V+QLAGSV +LSAYH E+SLMS I LAQ+FVGSNN+N+L PIGQFGTRL GGKD A PRY++T LN LT+LIFH +D +LN + DDN + EPEWYAPIIPM+L+NG IG G+++ I ++ I++I+ L + ++GE ++ P +R FTGTI + ++
Sbjct: 25 IPKLDDANNAGTKNSSQCTLILTEGDSAKTLAVAGLGVVGRDNYGVFPLRGKLLNVREASNKQIMENAEINALIKILGLQYKLKYESADTLKDLRYGKIMIMTDQDQDGSHIKGLIINFVHCNWPNLLKHNVVE-----EFITPIVKVFKGKQEYSFYSLPEFEEWQKSTPNWHTWRVKYYKGLGTSTSKEAKEYFSDMNRHRIRFRYSGTEDDGSIQLAFDKSKIADRKNWLTNFTQERKRRRELGLPEPYLYGKDTRAITYHDFVHKELVLFSNLDNERSIPSVVDGLKPGQRKVLFTCLKRNLIREIKVAQLAGSVAELSAYHHGEQSLMSTIIGLAQNFVGSNNLNLLQPIGQFGTRLSGGKDAASPRYIFTALNSLTRLIFHLEDDPLLNYLYDDNQR---IEPEWYAPIIPMVLVNGADGIGTGYATHILNYNVIEIINNLYRMLDGEEPHRMLPNFRGFTGTIEDLGNNRY 494
BLAST of DNA topoisomerase 2 vs. TrEMBL
Match: A0A401PNB4 (DNA topoisomerase 2 OS=Scyliorhinus torazame OX=75743 GN=scyTo_0003731 PE=3 SV=1) HSP 1 Score: 498.434 bits (1282), Expect = 2.571e-157 Identity = 270/540 (50.00%), Postives = 358/540 (66.30%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQD---GK--KYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF----NNMTLFDECGVIRKYKDVEEILECFYLKRLEMYGKRKNY 1641
Q M N++ S + +P L DAN AGT S CTLILTEG SAK+ A+S L RD YGVFPL+GK+LNVR+ S + A+I N++KIIGL Y + Y +E LK LRYGKLMIMTD D +GSHIKGLLINFIH WP LL+HN FIE+F PIVK N + +FYSI E E+W+ ++ +WR+KYYKGLGT TS+EA EYF + +R+ I F+Y G +D +I LAFS K +ERKEWL ++ + K L + GK KY+T +FI EL+L+ N+RSIPSL+DGL PG RKVLFTC + + E+ V+QLAGSV ++SAYH E SLM I LAQ+FVGSNNIN+L PIGQFGTRL+GGKD A PRY++T L+ L + +F D AIL M DDN + EPEWY PIIPM+L+NG IG GW+ IP + ++++ ++ ++G++ V + P Y+NF GTI E ++ LFD G ++KY++V++IL+ F+ RL+ Y RK++
Sbjct: 421 AQNMLNKKCSGLKQTKIKGIPKLDDANDAGTKHSQGCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLKGKILNVREASHKQIMENAEINNVIKIIGLQYKKDYNDQESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHN-----FIEEFITPIVKVSKNKQELAFYSIPEFEEWKSNVDNSRTWRIKYYKGLGTSTSKEAKEYFAEMQRHRIPFKYGGPEDDAAITLAFSRKKIDERKEWLTNFMVDRRQRKIDGLPEQYLYGKETKYLTYNEFINKELVLFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEISAYHHGEASLMMTIVGLAQNFVGSNNINVLQPIGQFGTRLNGGKDAASPRYIFTMLSTLARRLFPSVDDAILKYMDDDNQR---VEPEWYIPIIPMVLVNGADGIGTGWACRIPNYDVREIVNNIRHMLDGDDPVPMLPNYKNFKGTIFELGANQYMINGEISVLFDHTGCLKKYENVQDILKEFFNLRLKYYDLRKDW 952
BLAST of DNA topoisomerase 2 vs. TrEMBL
Match: A0A402FGU0 (DNA topoisomerase 2 OS=Paroedura picta OX=143630 GN=parPi_0018086 PE=3 SV=1) HSP 1 Score: 499.975 bits (1286), Expect = 3.518e-157 Identity = 269/559 (48.12%), Postives = 355/559 (63.51%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQD-----GKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKFN-----------------------NMTLFDECGVIRKYKDVEEILECFYLKRLEMYGKRKNY 1641
Q N++ S + +P L DAN AG+ +S CTLILTEG SAK+ A+S L RD YGVFPL+GK+LNVR+ S + A+I NL+KI+GL Y + Y+ E LK LRYGK+MIMTD D +GSHIKGLLINFIH WP LL+H F+E+F PIVK N + +FYSI E E+W+ P+ SW++KYYKGLGT TS+EA EYF + R+ I F+Y+G +D +I LAFS+ K +RKEWL ++E + K L ++ Y+T FI ELIL+ M N+RSIPSL+DGL PG RKV+FTC + + E+ V+QLAGSV ++SAYH E SLM I LAQ+FVGSNN+N+L PIGQFGTRL GGKD A PRY++T L+ L +L+F P D +LN + DDN + EPEWY PI+PM+LING IG GWS +P + +V+ L++ ++GE + +HP Y+NF GTI E +K++ LFD G ++KY E+IL FY RL YG RK++
Sbjct: 421 AQTQLNKKCSSVKHTRIKGIPKLDDANDAGSKNSINCTLILTEGDSAKTLAVSGLGIVGRDKYGVFPLRGKMLNVREASHKQIMENAEINNLIKIVGLQYKKNYDDPESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHR-----FLEEFITPIVKVSKNKEEVAFYSIPEFEEWKTTVPNYKSWKIKYYKGLGTSTSKEAKEYFAEMARHRIPFKYSGPEDDAAISLAFSKKKVEDRKEWLTNFMEDRRQRKLHGLPEEYLYGKATSYLTYNSFINKELILFSNMDNERSIPSLVDGLKPGQRKVMFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEASLMMTIINLAQNFVGSNNMNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLVFPPVDDNVLNFLYDDNRR---VEPEWYIPILPMVLINGAEGIGTGWSCKVPNYDVREVVHNLRRMLDGEEPLPMHPNYKNFKGTIDELGQKQYLVNGEVSILDATTIEISELPVRTWTQVLFDHVGCLKKYDTAEDILREFYELRLRYYGLRKDW 971
BLAST of DNA topoisomerase 2 vs. TrEMBL
Match: A0A0L8FFS9 (DNA topoisomerase 2 (Fragment) OS=Octopus bimaculoides OX=37653 GN=OCBIM_22023035mg PE=3 SV=1) HSP 1 Score: 496.508 bits (1277), Expect = 3.639e-157 Identity = 259/492 (52.64%), Postives = 340/492 (69.11%), Query Frame = 1
Query: 112 VPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLT-----KHLHLDQDGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF---NNMTLFDECGV 1557
+P L DAN+AGT +S CTLILTEG SAKS A++ L RD YGVFPL+GK+LNVR+ + + A+I NL+KI+GL + +KYE E L+ LRYGK+MIMTD D +GSHIKGL+INFIH +WP LLKHN +E +F PIVK N +FYSI E E+WQ+ T + +W++KYYKGLGT T++EA EYF D +R+ I F+Y G +D SI+LAFS+ K +ERKEWL ++E K L+L G I+ +FI ELIL+ N RSIPSL+DGL PG RKV+FTC + + +E+ V+QLAGSV +LSAYH E SLMS I LAQ+FVGSNNIN+L+PIGQFGTRL GGKD A PRY++T L+ + K IFH DD +L + DDN K EPE+Y PIIPMIL+NG IG GWS+ IP + P ++I +++ ++G + + + PW++NF GTI E SE K+ +++ DE V
Sbjct: 449 IPKLDDANWAGTKNSSHCTLILTEGDSAKSLAVAGLGIVGRDCYGVFPLKGKVLNVREATHKQIMENAEINNLIKILGLQFKEKYEDIEKLRTLRYGKIMIMTDQDNDGSHIKGLIINFIHHFWPNLLKHNVVE-----EFITPIVKVTKNKVEKAFYSIPEFEEWQRGTQNWHTWKIKYYKGLGTSTAKEAKEYFSDMERHRIPFKYEGPEDDASINLAFSKKKIDERKEWLTNWMEEKRRRLEMGLPELYLYGKGVNQISFNEFINRELILFSFTDNNRSIPSLVDGLKPGQRKVIFTCFKRNDKKEVKVAQLAGSVAELSAYHHGEASLMSTIINLAQNFVGSNNINLLLPIGQFGTRLHGGKDAASPRYIFTLLSSVGKRIFHSDDLPLLKNLYDDNQK---IEPEYYVPIIPMILVNGTDGIGTGWSTKIPNYDPREIITNIRRMLSGNDPLNMVPWFKNFKGTIEELSENKYLVSGEISVLDETTV 932
BLAST of DNA topoisomerase 2 vs. Ensembl Cavefish
Match: top2b (DNA topoisomerase II beta [Source:ZFIN;Acc:ZDB-GENE-041008-136]) HSP 1 Score: 466.848 bits (1200), Expect = 6.055e-147 Identity = 243/497 (48.89%), Postives = 332/497 (66.80%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQD-----GKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q N++ S + +P L DAN AG S ECTLILTEG SAKS A+S L RD YGVFPL+GK+LNVR+ + + A+I N++KI+GL Y + Y+ E LK LRYGK+MIMTD D +GSHIKGLLINF H WP LLKH F+E+F PIVKA N + FYSI E ++W+K+T + +W +KYYKGLGT TS+EA EYF D +R+ I F+Y G +D +I LAFS+ KT++RKEWL ++E + + L + ++++ FI ELIL+ N+RSIPSL+DGL PG RKVLFTC++ + E+ V+QLAGSV ++SAYH E++LM I LAQ+FVGSNN+NIL P+GQFGTR++GGKD A PRY++T L+ L KL+F D +L + DDN K EPEWY PIIPM+L+NG IG GW+ IP + P ++++ + + ++ ++ + + P Y+NF G I E + ++
Sbjct: 439 AQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSSECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREATHKQIMENAEINNIIKIVGLQYKKSYDDPESLKSLRYGKIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHT-----FLEEFITPIVKASKNKQEIPFYSIPEFDEWKKQTENYKTWHIKYYKGLGTSTSKEAKEYFADMERHRIMFKYGGTEDDAAITLAFSKKKTDDRKEWLTNFMEDRRQRRMHGLPEQYLYGTATRHLSYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCMKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVNILQPLGQFGTRINGGKDAASPRYIFTMLSPLAKLLFPAVDSNLLKFLYDDNQK---VEPEWYIPIIPMVLVNGAEGIGTGWACKIPNYDPREIVNNINRMLDHQDPLPMLPSYKNFKGVIHELGQNQY 927
BLAST of DNA topoisomerase 2 vs. Ensembl Cavefish
Match: top2a (DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GENE-030131-2453]) HSP 1 Score: 416.772 bits (1070), Expect = 4.257e-129 Identity = 229/486 (47.12%), Postives = 309/486 (63.58%), Query Frame = 1
Query: 70 NRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQD---GKKY--ITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAE 1506
N++ S + VP L DAN AG S CTLILTEG SAK+ A+S L RD YGVFPL+GK+LNVR+ S + A+I N++KI+GL Y + Y + LK LRYGKLMIMTD D +GSHIKGLLINFIH WP LL+HN F+E+F PI+K + N+ SFYSI E +W+++ + SW++KYYKGLGT TS+EA EYF D R+ I FRY+G D +I LAFS K ERK+WL ++ + + +L ++ GK+ +T F+ EL+L+ N+RSIP L+DGL PG RKVLF C + + E+ V+QLAGSV ++SAYH E SLM I + +P+GQFGTRL GGKD A PRY++T L+ L++L+F D +L DDN V+ EPEWY PIIP++L+NG IG GW+ +P F ++I+ + + +NG+ + + P Y+ F GTI E
Sbjct: 423 NKKCSAVKHTKIKGVPKLDDANDAGGKHSAGCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKMLNVREASHKQIMENAEINNIIKIMGLQYRKNYSDPDSLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHN-----FLEEFITPIIKVSSKNQEFSFYSIPEFTEWKEKQNNIKSWKIKYYKGLGTSTSKEAKEYFSDMARHRIPFRYSGPADDEAITLAFSRKKIEERKDWLTSFMINRRQRREHNLPEEYLYGKETSSLTYHDFVNKELVLFSNSDNERSIPCLVDGLKPGQRKVLFCCFKRNDKREVKVAQLAGSVAEMSAYHHGEISLMMTI--------------VGLPLGQFGTRLHGGKDSASPRYIFTMLSSLSRLLFPSVDDNLLKYNYDDN---VKVEPEWYIPIIPLVLVNGGDGIGTGWACKLPNFDVREIINNIHRMLNGDEPLPMLPSYKGFKGTIEE 886
BLAST of DNA topoisomerase 2 vs. Ensembl Cavefish
Match: top2a (DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GENE-030131-2453]) HSP 1 Score: 416.387 bits (1069), Expect = 6.956e-129 Identity = 229/486 (47.12%), Postives = 309/486 (63.58%), Query Frame = 1
Query: 70 NRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQD---GKKY--ITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAE 1506
N++ S + VP L DAN AG S CTLILTEG SAK+ A+S L RD YGVFPL+GK+LNVR+ S + A+I N++KI+GL Y + Y + LK LRYGKLMIMTD D +GSHIKGLLINFIH WP LL+HN F+E+F PI+K + N+ SFYSI E +W+++ + SW++KYYKGLGT TS+EA EYF D R+ I FRY+G D +I LAFS K ERK+WL ++ + + +L ++ GK+ +T F+ EL+L+ N+RSIP L+DGL PG RKVLF C + + E+ V+QLAGSV ++SAYH E SLM I + +P+GQFGTRL GGKD A PRY++T L+ L++L+F D +L DDN V+ EPEWY PIIP++L+NG IG GW+ +P F ++I+ + + +NG+ + + P Y+ F GTI E
Sbjct: 423 NKKCSAVKHTKIKGVPKLDDANDAGGKHSAGCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKMLNVREASHKQIMENAEINNIIKIMGLQYRKNYSDPDSLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHN-----FLEEFITPIIKVSSKNQEFSFYSIPEFTEWKEKQNNIKSWKIKYYKGLGTSTSKEAKEYFSDMARHRIPFRYSGPADDEAITLAFSRKKIEERKDWLTSFMINRRQRREHNLPEEYLYGKETSSLTYHDFVNKELVLFSNSDNERSIPCLVDGLKPGQRKVLFCCFKRNDKREVKVAQLAGSVAEMSAYHHGEISLMMTI--------------VGLPLGQFGTRLHGGKDSASPRYIFTMLSSLSRLLFPSVDDNLLKYNYDDN---VKVEPEWYIPIIPLVLVNGGDGIGTGWACKLPNFDVREIINNIHRMLNGDEPLPMLPSYKGFKGTIEE 886
BLAST of DNA topoisomerase 2 vs. Ensembl Sea Lamprey
Match: top2a (DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GENE-030131-2453]) HSP 1 Score: 479.559 bits (1233), Expect = 2.638e-155 Identity = 248/481 (51.56%), Postives = 332/481 (69.02%), Query Frame = 1
Query: 112 VPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYK---GLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQD---GKK--YITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
+P L DAN AG +S +CTLILTEG SAK+ A+S L RD YG+FPL+GK+LNVR+ S + A+I NL+KI+GL Y +KY+ E LK LRYGK+MIMTD D +GSHIKGLLINFIH WP LL+H F+E+F PIVK N + FYS+ E ++W+ ET + +W++KYYK GLGT TS+EA EYF D KR+ I+FRY+G +D N+I LAFS K +RKEWL ++ + + L + GK+ Y+T FI ELIL+ N+RSIPSL+DG PG RKVLFTC + + E+ V+QLAGSV ++SAYH E+SLM I LAQ+FVGSNNIN+L PIGQFGTRL+GGKD A PRY++T ++ + +L+F P D +L + DDN + EPEWY PIIP++L+NG IG GW+S IP F+P ++I+ +++ ++GE + + PWY+NF G+I E +F
Sbjct: 83 IPKLDDANDAGGKNSQKCTLILTEGDSAKTLAVSGLGVVGRDHYGIFPLRGKMLNVREASYKQIMENAEINNLIKIVGLQYKKKYDDPESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLQHR-----FLEEFITPIVKVSKNKQELPFYSMPEFDEWKNETSNYKTWKIKYYKDMPGLGTSTSKEAKEYFADMKRHRIEFRYSGPEDDNAITLAFSRKKIEDRKEWLTNWMVDRRQRREQGLPESFLYGKQTAYLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQSLMMTIVSLAQNFVGSNNINVLQPIGQFGTRLNGGKDAASPRYIFTMISTVARLLFPPVDDNLLKFLWDDNQR---VEPEWYCPIIPLVLVNGADGIGTGWASRIPNFNPREIINNIRRLLDGEEPLPMLPWYKNFKGSIDELGPNQF 555
BLAST of DNA topoisomerase 2 vs. Ensembl Yeast
Match: TOP2 (Topoisomerase II; relieves torsional strain in DNA by cleaving and re-sealing phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation; human homolog TOP2A implicated in cancers, and can complement yeast null mutant [Source:SGD;Acc:S000005032]) HSP 1 Score: 394.815 bits (1013), Expect = 2.823e-122 Identity = 222/483 (45.96%), Postives = 305/483 (63.15%), Query Frame = 1
Query: 73 RESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVS---SFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQDGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAE 1506
++S T + N P L DAN AGT + ++CTL+LTEG SA S A++ L RD+YG +PL+GK+LNVR+ S + A+I+ + KI+GL + +KYE K LRYG LMIMTD D +GSHIKGL+INF+ +P LL F+ +F PI+K +FY++ + EKW++E +W+ KYYKGLGT ++E EYF + R++ F D + IDLAFS+ K ++RKEWL +Y L L K I I FI ELIL+ + N RSIP+++DG PG RKVL+ C + +L E+ V+QLA V + +AYH E+SL I LAQ+FVGSNNI +L+P G FGTR GGKD A RY+YT+LNKLT+ IFHP D L + + ++ K V EPEWY PI+PMIL+NG IG GWS+ IP F+P+++I ++ +N E ++HPW+R +TGTI E
Sbjct: 413 KKSDGTRKSRITNYPKLEDANKAGTKEGYKCTLVLTEGDSALSLAVAGLAVVGRDYYGCYPLRGKMLNVREASADQILKNAEIQAIKKIMGLQHRKKYED---TKSLRYGHLMIMTDQDHDGSHIKGLIINFLESSFPGLLDIQ----GFLLEFITPIIKVSITKPTKNTIAFYNMPDYEKWREEESHKFTWKQKYYKGLGTSLAQEVREYFSNLDRHLKIFHSLQGNDKDYIDLAFSKKKADDRKEWLRQYEPGTVLDPTL-------KEIPISDFINKELILFSLADNIRSIPNVLDGFKPGQRKVLYGCFKKNLKSELKVAQLAPYVSECTAYHHGEQSLAQTIIGLAQNFVGSNNIYLLLPNGAFGTRATGGKDAAAARYIYTELNKLTRKIFHPADDP-LYKYIQEDEKTV--EPEWYLPILPMILVNGAEGIGTGWSTYIPPFNPLEIIKNIRHLMNDEELEQMHPWFRGWTGTIEE 878
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Match: EDO29547 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T3B9]) HSP 1 Score: 147.517 bits (371), Expect = 3.016e-41 Identity = 76/167 (45.51%), Postives = 109/167 (65.27%), Query Frame = 1
Query: 592 LEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTK-----HLHLDQDGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSE 1077
+E+W+ T + SW++KYYKGLGT T +EA EYF D +R+ I F+Y G D +I +AFS+ K ERK+WL + +E + + L+L + K ++ F+ ELIL+ N+RSIPSL+DGL PG RKV+FTC + + E+ V+QLAGS+ +LSAYH E
Sbjct: 1 MEEWKAGTANHKSWKVKYYKGLGTSTPKEAKEYFSDMERHKINFKYEGPHDDEAIVMAFSKKKIQERKDWLTRGLEERKWRREQGLSELYLYEKDTKRVSYCDFVNKELILFSNTDNERSIPSLVDGLKPGQRKVMFTCFKRNDKREVKVAQLAGSIAELSAYHHGE 167
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Match: EDO44812 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUU4]) HSP 1 Score: 130.183 bits (326), Expect = 9.550e-36 Identity = 65/118 (55.08%), Postives = 82/118 (69.49%), Query Frame = 1
Query: 301 NRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKG 654
+ A+I N++KI GL YN+KY+S++ LK LRYG LMIMTD D +GSHIKGL+INFIH WP LLK F+EQF PIVK + SFYSI E E+W+ + SW++KYYKG
Sbjct: 2 DNAEINNIIKITGLQYNKKYKSEDDLKSLRYGHLMIMTDQDQDGSHIKGLVINFIHSNWPGLLK-----LGFVEQFITPIVKVSKGKEEHSFYSIPEYEEWKAGNANHKSWKVKYYKG 114
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Match: EDO44811 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUU3]) HSP 1 Score: 85.8853 bits (211), Expect = 6.213e-18 Identity = 39/108 (36.11%), Postives = 66/108 (61.11%), Query Frame = 1
Query: 1243 IFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKFN---NMTLFDECGV 1557
+F P D+ L + +DN + EPEWY PIIP IL+NG IG G+S+ +P + P + + +++ +NGE ++ PWY+NF GTI + + K++ N+ + D+ +
Sbjct: 9 LFPPSDEPNLKFLNEDNQRI---EPEWYCPIIPTILVNGAEGIGTGYSTKVPNYDPRAIAENIRKMMNGEEPEEMLPWYKNFIGTITKVDDTKYSVTGNIEVIDDTTL 113
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Match: EDO44813 (DNA topoisomerase 2 [Source:UniProtKB/TrEMBL;Acc:A7RUU5]) HSP 1 Score: 82.0333 bits (201), Expect = 2.161e-16 Identity = 41/74 (55.41%), Postives = 51/74 (68.92%), Query Frame = 1
Query: 70 NRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS 291
N++ S + + +P L DAN AG +S CTLILTEG SAK+ AIS L+ RD YGVFPL+GKLLNVR+ S
Sbjct: 439 NKKCSGSKHSKIRGIPKLDDANDAGGKNSRHCTLILTEGDSAKTLAISGLSIVGRDRYGVFPLRGKLLNVREAS 512
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Match: EDO25698 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8DVR1]) HSP 1 Score: 72.7886 bits (177), Expect = 3.707e-14 Identity = 61/231 (26.41%), Postives = 106/231 (45.89%), Query Frame = 1
Query: 151 DSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGSE---FRNRAKIENLVKIIGLDYNQKYESKEL-LKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRL------KYYKGLGTFTSEEAGEYFRDKK-RNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEK 810
D +C + L EG SA A DR+F + PL+GK+LNV K + F N +I+N+ +G+ + + + L L +RY K++IM D D +GSHI L++ F Y E+ IE ++ PP+ K Y+ W D + ++ + YKGLG +E+ + + + R + + + + + + D+ R+E++EK
Sbjct: 59 DPAQCEIFLVEGDSAGGTAKQ---GRDRNFQAILPLRGKILNVEKAMQHKVFENE-EIKNMFTALGVSIGTEEDPRALNLSKVRYHKVVIMCDADVDGSHIATLILTFFFRYMREM-----IEQGYVYIATPPLYLVKKGQKRE--YA------WDDNQRDLIAQKMGGGVTIQRYKGLGEMNAEQLWDTTMNPEFRTLRQVTIDNMTEADRVFSMLMGDEVPPRREFIEK 272
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Match: top2b (DNA topoisomerase II beta [Source:NCBI gene;Acc:101157229]) HSP 1 Score: 475.707 bits (1223), Expect = 9.173e-151 Identity = 251/497 (50.50%), Postives = 334/497 (67.20%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQ-----DGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q N++ S + +P L DAN AG S ECTLILTEG SAKS A+S L RD YGVFPL+GK+LNVR+ + + A+I N++KI+GL Y + Y+ E L+ LRYGK+MIMTD D +GSHIKGLLINF H WP LLKH F+EQF PIVKA N + SFYS+ E E+W+K+T + SW +KYYKGLGT TS+EA EYF D +R+ I FRY G +D +I LAFS+ KT++RKEWL ++E + + L + K+++ FI ELIL+ N+RSIPSL+DGL PG RKVLFTCL+ + E+ V+QLAGSV ++SAYH E++LM I LAQ+FVGSNNINIL P+GQFGTR++GGKD A PRY++T L+ LTKL+F D +L + DDN K EPEWY PIIPM+L+NG IG GW+ IP + P ++++ + + +N ++ + + P Y+NF G I E + ++
Sbjct: 350 AQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSSECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREATHKQIMENAEINNIIKIVGLQYKKSYDDPESLRSLRYGKIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHT-----FLEQFITPIVKATKNKQELSFYSLPEFEEWKKQTDNYKSWHVKYYKGLGTSTSKEAKEYFADMERHRITFRYQGAEDDAAITLAFSKKKTDDRKEWLTNFMEDRRQRRMHGLPEKYLYGTQTKHLSYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCLKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINILQPLGQFGTRINGGKDAASPRYIFTMLSPLTKLLFPAVDSNLLKFLYDDNQK---VEPEWYIPIIPMVLVNGAEGIGTGWACKIPNYDPREIVNNINRMLNHQDPLPMLPSYKNFKGVIHELGQNQY 838
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Match: top2b (DNA topoisomerase II beta [Source:NCBI gene;Acc:101157229]) HSP 1 Score: 475.322 bits (1222), Expect = 4.249e-150 Identity = 253/499 (50.70%), Postives = 336/499 (67.33%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLT-------KHLHLDQDGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q N++ S + +P L DAN AG S ECTLILTEG SAKS A+S L RD YGVFPL+GK+LNVR+ + + A+I N++KI+GL Y + Y+ E L+ LRYGK+MIMTD D +GSHIKGLLINF H WP LLKH F+EQF PIVKA N + SFYS+ E E+W+K+T + SW +KYYKGLGT TS+EA EYF D +R+ I FRY G +D +I LAFS+ KT++RKEWL ++E + K+L+ Q K+++ FI ELIL+ N+RSIPSL+DGL PG RKVLFTCL+ + E+ V+QLAGSV ++SAYH E++LM I LAQ+FVGSNNINIL P+GQFGTR++GGKD A PRY++T L+ LTKL+F D +L + DDN K EPEWY PIIPM+L+NG IG GW+ IP + P ++++ + + +N ++ + + P Y+NF G I E + ++
Sbjct: 429 AQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSSECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREATHKQIMENAEINNIIKIVGLQYKKSYDDPESLRSLRYGKIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHT-----FLEQFITPIVKATKNKQELSFYSLPEFEEWKKQTDNYKSWHVKYYKGLGTSTSKEAKEYFADMERHRITFRYQGAEDDAAITLAFSKKKTDDRKEWLTNFMEDRRQRRMHGLPEKYLYGTQ--TKHLSYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCLKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINILQPLGQFGTRINGGKDAASPRYIFTMLSPLTKLLFPAVDSNLLKFLYDDNQK---VEPEWYIPIIPMVLVNGAEGIGTGWACKIPNYDPREIVNNINRMLNHQDPLPMLPSYKNFKGVIHELGQNQY 917
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Match: top2a (DNA topoisomerase II alpha [Source:NCBI gene;Acc:101158729]) HSP 1 Score: 453.751 bits (1166), Expect = 3.091e-142 Identity = 241/499 (48.30%), Postives = 321/499 (64.33%), Query Frame = 1
Query: 55 TQFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQD-----GKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKFNN 1530
Q N++ S + VP L DAN AG +S CTLILTEG SAK+ A+S L RD YGVFPL+GK+LNVR+ S + A+I N++KI+GL Y + Y E LK LRYGK+MIMTD D +GSHIKGLLINFIH WP LL+HN F+E+F PI+KA N SFYSI E W++ + SW++KYYKGLGT TS+EA EYF D +R+ I F+Y+G +D +I LAFS+ K ERKEWL ++ + + +L +D K ++ FI EL+L+ N+RSIP L+DG PG RKVLF C + + E+ V+QLAGSV ++SAYH E SLM I LAQ+FVGSNN+N+L P+GQFGTRL GGKD A PRY++T L+ L +L+F P D +L DDN + EPEWY PIIP +L+NG IG GW+S IP + +++ + + ++GE + + P Y+ F GTI + ++ N
Sbjct: 415 AQTQLNKKCSAVKHTKIKGVPKLDDANDAGGKNSIGCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKMLNVREASHKQIMENAEINNVIKILGLQYKKNYSDPESLKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHN-----FLEEFITPIIKASNKKNQLSFYSIPEFNAWKETQSNHKSWKIKYYKGLGTSTSQEAKEYFSDMERHRIPFKYSGPEDDEAISLAFSKKKVEERKEWLTNFMVNRRQRREHNLPEDYLYGQSTKSLSYNDFINKELVLFSNSDNERSIPCLVDGFKPGQRKVLFCCFKRNDKREVKVAQLAGSVAEMSAYHHGEVSLMMTIVGLAQNFVGSNNLNLLQPLGQFGTRLHGGKDSASPRYIFTMLSPLARLVFPPVDDNLLKYNYDDNQR---VEPEWYIPIIPTVLVNGAEGIGTGWASKIPNYDIREIVSNIHRMLDGEEPLPMLPNYKGFRGTIDQVMSNQYMN 905
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Match: top2b (DNA topoisomerase II beta [Source:NCBI gene;Acc:101157229]) HSP 1 Score: 81.2629 bits (199), Expect = 5.441e-16 Identity = 35/94 (37.23%), Postives = 57/94 (60.64%), Query Frame = 1
Query: 1243 IFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
+F D +L + DDN K EPEWY PIIPM+L+NG IG GW+ IP + P ++++ + + +N ++ + + P Y+NF G I E + ++
Sbjct: 11 LFPAVDSNLLKFLYDDNQKV---EPEWYIPIIPMVLVNGAEGIGTGWACKIPNYDPREIVNNINRMLNHQDPLPMLPSYKNFKGVIHELGQNQY 101
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Match: SMESG000070153.1 (SMESG000070153.1) HSP 1 Score: 981.089 bits (2535), Expect = 0.000e+0 Identity = 484/497 (97.38%), Postives = 487/497 (97.99%), Query Frame = 1
Query: 64 MPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGSEFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQDGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKFNNMTLFDECG 1554
MPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGSEFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRY GEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQDGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVS+LAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRYLYTKLNKLTKLIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSE + D+ G
Sbjct: 1 MPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGSEFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYKGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQDGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSRLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRYLYTKLNKLTKLIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSENSYTVKGKVDKIG 497 HSP 2 Score: 137.887 bits (346), Expect = 1.347e-33 Identity = 89/116 (76.72%), Postives = 91/116 (78.45%), Query Frame = 1
Query: 1519 KFNNMTLFDECGVIRKYKDVEEILECFYLKRLEMYGKRKNYX--------------------XXXXXXXXXXXXXXXXRIKDKSKKEMWLEDLEKLENEIKVSYDSDIQESEEIEP 1806
+ +NMTLFDECGVIRKYKDVEEILECFYLKRLEMYGKRKNYL LKEIDDKLKELKRIKDKSKKEMWLEDLEKLENEIKVSYDSDIQESEEIEP
Sbjct: 645 RLSNMTLFDECGVIRKYKDVEEILECFYLKRLEMYGKRKNYLLDNLLKEIDDKLKELKEIDDKLKELKEIDDKLKELKRIKDKSKKEMWLEDLEKLENEIKVSYDSDIQESEEIEP 760
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Match: SMESG000037557.1 (SMESG000037557.1) HSP 1 Score: 593.964 bits (1530), Expect = 0.000e+0 Identity = 311/496 (62.70%), Postives = 366/496 (73.79%), Query Frame = 1
Query: 58 QFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQ-----DGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q + +++ S T + ++P L DANFAGT +S +CTLILTEG SAKS A+S L RD+YGVFPL+GKLLNVR+ S + A+I N++KIIGL Y+QKYE E LK LRYGKLMIMTD D +GSHIKGLLINFIH WP LLKHN F+EQF PIVKAF NN+V SFYSI EL +WQK TP A WRLKYYKGLGT T +EA EYF D +R++IKFRY+GE+DG SI LAFS+ K +ERKEWL ++E + L +D+ G KYIT FI ELIL+ N+RSIP LMDG PG RKVLF CL+ +LV+E+ V+QLAGSV +LSAYH E SLMS I LAQDFVGSNNIN+LMP GQFGTRL GGKD A PRY+ T+L+KLTKLIFH DD+A+L +LDDN EPEWYAPIIPM+LINGC IG GWSS IP F+P DVID LK+ ING VK+HPWYRNFTGTI E E K+
Sbjct: 327 QALLDKKCSKTKQTKLKDMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLKGKLLNVREASAKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHN-----FVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTDKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQ---SIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKY 814 HSP 2 Score: 57.3806 bits (137), Expect = 3.937e-8 Identity = 27/40 (67.50%), Postives = 31/40 (77.50%), Query Frame = 1
Query: 1522 FNNMTLFDECGVIRKYKDVEEILECFYLKRLEMYGKRKNY 1641
+NM LFDECGVIRKY + EILE F+ RLE+Y KRKNY
Sbjct: 904 LSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNY 943
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Match: SMESG000037557.1 (SMESG000037557.1) HSP 1 Score: 593.578 bits (1529), Expect = 0.000e+0 Identity = 311/496 (62.70%), Postives = 366/496 (73.79%), Query Frame = 1
Query: 58 QFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQ-----DGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q + +++ S T + ++P L DANFAGT +S +CTLILTEG SAKS A+S L RD+YGVFPL+GKLLNVR+ S + A+I N++KIIGL Y+QKYE E LK LRYGKLMIMTD D +GSHIKGLLINFIH WP LLKHN F+EQF PIVKAF NN+V SFYSI EL +WQK TP A WRLKYYKGLGT T +EA EYF D +R++IKFRY+GE+DG SI LAFS+ K +ERKEWL ++E + L +D+ G KYIT FI ELIL+ N+RSIP LMDG PG RKVLF CL+ +LV+E+ V+QLAGSV +LSAYH E SLMS I LAQDFVGSNNIN+LMP GQFGTRL GGKD A PRY+ T+L+KLTKLIFH DD+A+L +LDDN EPEWYAPIIPM+LINGC IG GWSS IP F+P DVID LK+ ING VK+HPWYRNFTGTI E E K+
Sbjct: 191 QALLDKKCSKTKQTKLKDMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLKGKLLNVREASAKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHN-----FVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTDKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQ---SIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKY 678 HSP 2 Score: 57.3806 bits (137), Expect = 3.757e-8 Identity = 27/40 (67.50%), Postives = 31/40 (77.50%), Query Frame = 1
Query: 1522 FNNMTLFDECGVIRKYKDVEEILECFYLKRLEMYGKRKNY 1641
+NM LFDECGVIRKY + EILE F+ RLE+Y KRKNY
Sbjct: 768 LSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNY 807
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Match: SMESG000037559.1 (SMESG000037559.1) HSP 1 Score: 565.074 bits (1455), Expect = 0.000e+0 Identity = 294/496 (59.27%), Postives = 346/496 (69.76%), Query Frame = 1
Query: 58 QFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQ-----DGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q + +++ S T + ++P L DANFAGT +S +CTLILTEG SAKS A+S L RD+YGVFPL+GKLLNVR+ S + A+I N++KIIGL Y+QKYE E LK LRYGKLMIMTD D +GSHIKGLLINFIH WP LLKHN F+EQF PIVKAF NN+V SFYSI EL +WQK TP A WRLKYYKGLGT T +EA EYF D +R++IKFRY+GE+DG SI LAFS+ K +ERKEWL ++E + L +D+ G KYIT FI ELIL+ N+RSIP LMDG PG RKVLF CL+ +LV+E+ V+QLAGSV +LSAYH E SLMS I LAQDFVGSNNIN+LMP GQFGTRL G K + H A+L +LDDN EPEWYAPIIPM+LINGC IG GWSS IP F+P DVID LK+ ING VK+HPWYRNFTGTI E E K+
Sbjct: 413 QALLDKKCSKTKQTKLKDMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLKGKLLNVREASAKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHN-----FVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTDKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGAK-----------------IALHLVTYAVLKYLLDDNQ---SIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKY 883 HSP 2 Score: 57.3806 bits (137), Expect = 3.418e-8 Identity = 27/40 (67.50%), Postives = 31/40 (77.50%), Query Frame = 1
Query: 1522 FNNMTLFDECGVIRKYKDVEEILECFYLKRLEMYGKRKNY 1641
+NM LFDECGVIRKY + EILE F+ RLE+Y KRKNY
Sbjct: 973 LSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNY 1012
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Match: SMESG000037565.1 (SMESG000037565.1) HSP 1 Score: 556.984 bits (1434), Expect = 0.000e+0 Identity = 289/496 (58.27%), Postives = 343/496 (69.15%), Query Frame = 1
Query: 58 QFMPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISCLTATDRDFYGVFPLQGKLLNVRKGS--EFRNRAKIENLVKIIGLDYNQKYESKELLKGLRYGKLMIMTDPDPEGSHIKGLLINFIHFYWPELLKHNTIECDFIEQFAPPIVKAFNNNKVSSFYSILELEKWQKETPDAASWRLKYYKGLGTFTSEEAGEYFRDKKRNVIKFRYNGEKDGNSIDLAFSEDKTNERKEWLEKYVECKDLTKHLHLDQ-----DGKKYITIRKFIKYELILYGIMHNKRSIPSLMDGLIPGPRKVLFTCLEMDLVEEMNVSQLAGSVIKLSAYHQSEESLMSNIKKLAQDFVGSNNINILMPIGQFGTRLDGGKDCALPRXXXXXXXXXXXXIFHPDDKAILNRMLDDNHKPVEPEPEWYAPIIPMILINGCTAIGYGWSSSIPCFHPIDVIDYLKQRINGENTVKIHPWYRNFTGTIAEKSEKKF 1524
Q + +++ S T + ++P L DANFAGT +S +CTLILTEG SAKS A+S L RD+YGVFPL+GKLLNVR+ + A+I N++KIIGL Y+QKYE E LK LRYGKLMIMTD D +GSHIKGLLINFIH WP LLKHN F+EQF PIVKAF NN+V SFYSI EL +WQ+ TPD+ WRLKYYKGLGT TS+EA EYF D +R++IKFRY+GE+DG SI LAFS+ K +ERKEWL ++E + L +D+ G KYIT FI ELIL+ N+RSIP LMDG PG RKVLF CL+ +LV+E+ V+QLAGSV +LSAYH E SLMS I LAQDFVGSNNIN+LMP GQFGTRL GGKD A PRY + + +WYAPIIPM+LINGC IG GWSS IP F+P DVID LK+ ING VK+HPWYRNFTGTI E E K+
Sbjct: 410 QALLDKKCSKTKHTKLKDIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHN-----FVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRY-------------------------------ICTQLKWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKY 869 HSP 2 Score: 57.3806 bits (137), Expect = 3.777e-8 Identity = 27/40 (67.50%), Postives = 31/40 (77.50%), Query Frame = 1
Query: 1522 FNNMTLFDECGVIRKYKDVEEILECFYLKRLEMYGKRKNY 1641
+NM LFDECGVIRKY + EILE F+ RLE+Y KRKNY
Sbjct: 959 LSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNY 998 The following BLAST results are available for this feature:
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 4
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of DNA topoisomerase 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 2
BLAST of DNA topoisomerase 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 3
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of DNA topoisomerase 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 2
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of DNA topoisomerase 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of DNA topoisomerase 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 3
BLAST of DNA topoisomerase 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of DNA topoisomerase 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 4
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30015481 ID=SMED30015481|Name=DNA topoisomerase 2|organism=Schmidtea mediterranea sexual|type=transcript|length=2055bpback to top protein sequence of SMED30015481-orf-1 >SMED30015481-orf-1 ID=SMED30015481-orf-1|Name=SMED30015481-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=582bp MPNRESSDTSAKVVVNVPNLVDANFAGTADSWECTLILTEGSSAKSFAISback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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