SMED30015480

Overview
NameSMED30015480
Smed IDSMED30015480
Length (bp)905
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30015480 (SMED30015480) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30015480 (SMED30015480) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30015480 (SMED30015480) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30015480 (SMED30015480) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30015480

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30015480h1SMcG0010262 SmedASXL_011045SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30015480h1SMcG0010262 dd_Smed_v4_16354_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30015480 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000465:154772..155676 -4525
Homology
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BLAST of SMED30015480 vs. TrEMBL
Match: H2KQ63 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CLF_103302 PE=4 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 4.700e-7
Identity = 56/269 (20.82%), Postives = 104/269 (38.66%), Query Frame = 3 
Query:  195 QRQIKTKLKFYKNYLRLSKISWFKNENEKTLAMEHIRSFYIFNHMKEFIILGL-ENCHQKKSYMVLKIKNINDMLELCYAIE-------NHDSEH-------------------LSKMGVQTQIET-----------------------------------------------RNREIMHYAITAELETHNIPRPVVKEMYQPETKIKQNYFQKSRMEETHFENNILESKFGESDIQWQEDIKFISYNPKTGNLVDNQGSVYMYTAQQI 779
            ++ IK KL      L LS+ SW+     +      +     F H+  F  +G+ ++   +K ++V+  K ++D+  + + +E       N ++ H                   L+  G+  QIET                                               R+RE   +++    E   +P     ++ +    I +N    +R  +     N L S+   +D  W+ D+K+I Y+P+ GN++DN+GSVY+YTA Q+
Sbjct:   54 KKPIKAKLLITYTSLILSRASWWPYRVVERTDFGAVERCMAFPHVGPFFAVGIRDDDFAEKQFVVMTTKTLSDLQRVMHVMEAQLTVWRNANTPHPSELSPPSSALRKCASQHDLAPAGLNNQIETPASNDYSSVPKKTDTTRLNHKQEANGDYLVQIRRCECPPKWLSRIYLRDREQPKHSVHIHCEPEKLPESKYTKLSKKAQHISENASTTNRPAQ----GNRLTSQQNSNDKDWEVDVKYIRYDPRFGNVLDNEGSVYLYTAHQV 318
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BLAST of SMED30015480 vs. TrEMBL
Match: A0A3S5A1Q5 (Uncharacterized protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS18814 PE=4 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 2.182e-6
Identity = 25/46 (54.35%), Postives = 32/46 (69.57%), Query Frame = 3 
Query:  669 ESDIQWQEDIKFISYNPKTGNLVDNQGSVYMYTAQQIVPQHRYNFG 806
            E +  W+ DIK ISY+P+ GN+VDN G +YM+ A QIVPQ R   G
Sbjct:  360 EDEGAWEVDIKHISYDPEVGNVVDNSGPIYMFVAHQIVPQKRNYLG 405
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BLAST of SMED30015480 vs. Planmine SMEST
Match: SMESG000064024.1 (SMESG000064024.1)

HSP 1 Score: 487.649 bits (1254), Expect = 3.589e-176
Identity = 263/263 (100.00%), Postives = 263/263 (100.00%), Query Frame = 3 
Query:   63 MKQQNKAERIPVTFITSKNLMEDETFSILSAHXXXXXXXXXXXXQRQIKTKLKFYKNYLRLSKISWFKNENEKTLAMEHIRSFYIFNHMKEFIILGLENCHQKKSYMVLKIKNINDMLELCYAIENHDSEHLSKMGVQTQIETRNREIMHYAITAELETHNIPRPVVKEMYQPETKIKQNYFQKSRMEETHFENNILESKFGESDIQWQEDIKFISYNPKTGNLVDNQGSVYMYTAQQIVPQHRYNFGXXXXXXXXXXXXLQL 851
            MKQQNKAERIPVTFITSKNLMEDETFSILSAHNLVVLFLNKKLKQRQIKTKLKFYKNYLRLSKISWFKNENEKTLAMEHIRSFYIFNHMKEFIILGLENCHQKKSYMVLKIKNINDMLELCYAIENHDSEHLSKMGVQTQIETRNREIMHYAITAELETHNIPRPVVKEMYQPETKIKQNYFQKSRMEETHFENNILESKFGESDIQWQEDIKFISYNPKTGNLVDNQGSVYMYTAQQIVPQHRYNFGSVSSSSSEDSKSLQL
Sbjct:    1 MKQQNKAERIPVTFITSKNLMEDETFSILSAHNLVVLFLNKKLKQRQIKTKLKFYKNYLRLSKISWFKNENEKTLAMEHIRSFYIFNHMKEFIILGLENCHQKKSYMVLKIKNINDMLELCYAIENHDSEHLSKMGVQTQIETRNREIMHYAITAELETHNIPRPVVKEMYQPETKIKQNYFQKSRMEETHFENNILESKFGESDIQWQEDIKFISYNPKTGNLVDNQGSVYMYTAQQIVPQHRYNFGSVSSSSSEDSKSLQL 263
[Close]
BLAST of SMED30015480 vs. Planmine SMEST
Match: SMESG000024438.1 (SMESG000024438.1)

HSP 1 Score: 51.2174 bits (121), Expect = 3.064e-7
Identity = 66/257 (25.68%), Postives = 114/257 (44.36%), Query Frame = 3 
Query:  123 MEDETFSILSAHXXXXXXXXXXXXQRQIKTKLKFYKNYLRLSKISWFKNENEKTLAMEHIRSFYIFNHMKEFIILGLENCHQKKSYMVLKIKNINDMLELCYAIE---------NHDSEHLSKMGVQTQIETRNREIMHYAI--TAELETHNIPRPVV------------KEMYQPETKIKQNYFQKSR----MEETHFENNILESKFGESDI-QWQEDIKFISYNPKTGNL-VDNQGSVYMYTAQQIVPQHRYNFG 806
            +++ TF++ SAHNL+    +KK     +K KL  Y + ++++K + F   ++K   +  +R F++F      I  G+E   +K  Y+V+  + ++ + E+C  IE         N D E   ++ V  Q++    E++   +    EL  H      V            KE+  P  +    Y    R    M  T  +  +L +   + D  QW+ED+++  YNP  G   V   G ++MYTA    P    NFG
Sbjct:   22 VKNTTFTLESAHNLLKSLQSKKS-DHSVKAKLNVYDSGMKITKKAPF-GRSQKVPFLSVLR-FFVFKTEPHLIFFGVEK-RKKLFYLVILFRELDQVNEICDLIERKRGGAVKVNTDPE--PQISVCQQVK---EEVIPICVEPVVELVNHGTQSEPVEEKVVYIQSTEKKEIRTPIVRRNLEYHYDERHTDDMGATRVDALLLRTNKDKIDSKQWKEDMQYFDYNPDIGTATVSEVGPIFMYTA-HFTPAEYNNFG 268
The following BLAST results are available for this feature:
BLAST of SMED30015480 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015480 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015480 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015480 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015480 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015480 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015480 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015480 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 2
Match NameE-valueIdentityDescription
H2KQ634.700e-720.82Uncharacterized protein OS=Clonorchis sinensis OX=... [more]
A0A3S5A1Q52.182e-654.35Uncharacterized protein OS=Protopolystoma xenopodi... [more]
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BLAST of SMED30015480 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015480 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015480 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015480 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015480 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015480 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000064024.13.589e-176100.00SMESG000064024.1[more]
SMESG000024438.13.064e-725.68SMESG000024438.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015480 ID=SMED30015480|Name=SMED30015480|organism=Schmidtea mediterranea sexual|type=transcript|length=905bp
AATTAACAACATTCTTAAACTGTTTTGGTTGTATCTAAAGATTTTTTCAA
ATAACTGAGGACATGAAACAACAGAATAAAGCGGAAAGAATTCCAGTGAC
ATTTATTACGAGCAAAAATCTGATGGAGGATGAGACTTTCTCCATCCTTT
CTGCCCATAACCTCGTAGTGCTGTTTCTGAATAAAAAATTAAAACAACGT
CAAATAAAGACCAAACTAAAATTTTACAAAAATTATTTGAGACTGTCGAA
AATTTCCTGGTTCAAGAATGAAAATGAAAAGACTTTAGCGATGGAACACA
TAAGAAGTTTTTATATTTTCAATCATATGAAAGAGTTTATAATTTTGGGG
CTGGAGAATTGTCATCAGAAAAAATCATATATGGTCTTGAAAATAAAAAA
TATAAACGATATGTTAGAGTTGTGCTATGCTATTGAAAATCATGATTCTG
AACATTTATCGAAAATGGGCGTTCAAACTCAAATCGAAACGAGAAATCGA
GAAATCATGCATTACGCCATCACGGCTGAACTGGAAACTCATAATATTCC
TAGACCAGTAGTCAAGGAAATGTATCAACCTGAAACAAAGATAAAGCAAA
ATTATTTCCAAAAGTCTAGAATGGAAGAAACTCATTTCGAAAATAACATT
TTAGAAAGCAAATTCGGTGAAAGCGACATTCAATGGCAGGAAGATATCAA
ATTTATTTCCTATAATCCAAAAACGGGGAATTTAGTTGATAATCAAGGCT
CAGTTTATATGTACACAGCTCAGCAGATTGTTCCTCAACATCGGTATAAT
TTCGGATCTGTTTCATCATCAAGTTCTGAAGATTCCAAATCTTTACAACT
TTAAATATATTCAACCAAAATTAAATATTTGAATATTACTTAATATTTTT
TTTAC
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protein sequence of SMED30015480-orf-1

>SMED30015480-orf-1 ID=SMED30015480-orf-1|Name=SMED30015480-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=264bp
MKQQNKAERIPVTFITSKNLMEDETFSILSAHNLVVLFLNKKLKQRQIKT
KLKFYKNYLRLSKISWFKNENEKTLAMEHIRSFYIFNHMKEFIILGLENC
HQKKSYMVLKIKNINDMLELCYAIENHDSEHLSKMGVQTQIETRNREIMH
YAITAELETHNIPRPVVKEMYQPETKIKQNYFQKSRMEETHFENNILESK
FGESDIQWQEDIKFISYNPKTGNLVDNQGSVYMYTAQQIVPQHRYNFGSV
SSSSSEDSKSLQL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000420parapharyngeal region