G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30015471
Length (bp)1483
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30015471) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30015471) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30015471

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 16

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30015471h1SMcG0017827 SmedASXL_005349SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30015471h1SMcG0017827 dd_Smed_v4_6156_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30015471h1SMcG0017827 dd_Smed_v4_6156_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30015471h1SMcG0017827 dd_Smed_v4_6156_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30015471h1SMcG0017827 dd_Smed_v4_6156_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30015471h1SMcG0017827 dd_Smed_v4_6156_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30015471h1SMcG0017826 dd_Smed_v4_6156_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30015471h1SMcG0017826 dd_Smed_v4_6156_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30015471h1SMcG0017826 dd_Smed_v4_6156_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30015471h1SMcG0017826 dd_Smed_v4_6156_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30015471h1SMcG0017826 dd_Smed_v4_6156_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30015471h1SMcG0017827 dd_Smed_v4_43803_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
tail regionSMED30015471h1SMcG0017827 dd_Smed_v6_6156_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
tail regionSMED30015471h1SMcG0017826 dd_Smed_v6_6156_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
intestinal phagocyteSMED30015471h1SMcG0017827 dd_Smed_v6_6156_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30015471h1SMcG0017826 dd_Smed_v6_6156_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Match: frpr-8 (FMRFamide Peptide Receptor family [Source:UniProtKB/TrEMBL;Acc:Q20686])

HSP 1 Score: 52.373 bits (124), Expect = 5.258e-7
Identity = 44/158 (27.85%), Postives = 78/158 (49.37%), Query Frame = 3 
Query:  186 IEVLSLYV---QIP-ICVIGCILNMVNFAIFVQQDFSSATYVLITALSLTD----SFT-LFLRVGQGLIRCSSEGWCNNPYFRFFLYIFIVFLDIPITNTTESISSWLTMALAIERCLTMRWYHLSKVYCTIKNARNLIIAISFFATILNLPFFFTLS 632
            ++ +S Y+    IP ICV+G    ++   +F ++   S+  V +  LS+ D    SF+ L     QG   C  +G  +     FF         +PI+N  +  S W  +A+ ++R L + +   SK++CT + A  ++IAI+ F+ +   P FF L+
Sbjct:   39 VDYISTYIYLLAIPTICVLGAGAAVMCIVVFTRKQMRSSLNVYLAGLSVFDLILLSFSALIFSPLQG---CVLQGHGDTAVCHFFWRS--TPWTLPISNIAQCGSVWTCVAVTVDRFLAVNYPLHSKIWCTPRRATTILIAITVFSILFKAPMFFELT 191
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A068WK46 (G protein coupled receptor OS=Echinococcus granulosus OX=6210 GN=EgrG_000234800 PE=4 SV=1)

HSP 1 Score: 190.274 bits (482), Expect = 6.087e-52
Identity = 135/384 (35.16%), Postives = 212/384 (55.21%), Query Frame = 3 
Query:   96 KQIVTGMVENIKSLSFIGDEYFPQSSWKIAIEVLSL---YVQIPICVIGCILNMVNFAIFVQQDF-SSATYVLITALSLTDSFTLFLRVGQGLI--RCSSEGWCNNPYFRFFLYIFIVFLDIPITNTTESISSWLTMALAIERCLTMRWYHLSKVYCTIKNARNLIIAISFFATILNLPFFFTLSLNIXXXXXXXXXXXXXVMVPVITSQWTSFAENSTYYLIHSWTRTVLVQYVPFIFLCVSNVLLFTFLRTHNRRFVRASIKKHHSELLAANKCKGILSDSYKSYKLSNSASQLAVRKQK---AQKKXXXXXXXXXXXXXXXXXPHGFAYVKIYQTIGSCPSYNKIDECTEYQMYRTITMILTLLGYSANFFVYFVLNLHFR 1220
            K IV GM    +        Y  ++  +  + + ++   YVQ  IC+IG ILN++N  +F ++ F S+  Y+L+ ALS+ D+ TL LR+ QGL   R SS           + YI+  + ++PITN TE++S+WLT+ LA+ER + MR ++ ++ Y T +N + LII+I+  + + NLP+F    +      + S        + VIT+   +    ST+Y ++SW R  +VQ +P +FLC+ NVLL T +  HN++F + +  +  S  L+    +G  +D       +    QL  R+QK   AQ+KL ILLI VILLFL   IP  F+Y++I++  G+C        C  Y +YR +T  + L+ Y   FF+YF LN HFR
Sbjct:    3 KNIVGGMAGTSRGGEAALRAYLQRADVQQGLRISAIGYVYVQPCICLIGFILNIINIYVFQRKTFCSTPAYMLVLALSIADAITLGLRIPQGLTYYRGSSS---VTQTMAIYAYIYATYAEVPITNMTENMSAWLTVCLALERYVAMRHWNFARQYSTRQNTKRLIISIALVSVLFNLPYFMIHRIEFKRFANGS--------MRVITN--ATALRRSTFYTVYSWFRMTIVQIIPLVFLCILNVLLITIVYQHNKKFGKNTEVEQKSNHLSLGT-EGTGADETSLKDTTVGDLQLIQRRQKKRNAQRKLNILLIAVILLFLFGAIPQAFSYIRIFEAFGTCEE--AFHYCPTYVVYRMVTTNIALISYGLTFFLYFFLNQHFR 370
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: W6UXS1 (Putative G-protein coupled receptor OS=Echinococcus granulosus OX=6210 GN=EGR_01868 PE=4 SV=1)

HSP 1 Score: 187.578 bits (475), Expect = 4.929e-51
Identity = 129/346 (37.28%), Postives = 199/346 (57.51%), Query Frame = 3 
Query:  201 LYVQIPICVIGCILNMVNFAIFVQQDF-SSATYVLITALSLTDSFTLFLRVGQGLI--RCSSEGWCNNPYFRFFLYIFIVFLDIPITNTTESISSWLTMALAIERCLTMRWYHLSKVYCTIKNARNLIIAISFFATILNLPFFFTLSLNIXXXXXXXXXXXXXVMVPVITSQWTSFAENSTYYLIHSWTRTVLVQYVPFIFLCVSNVLLFTFLRTHNRRFVRASIKKHHSELLAANKCKGILSDSYKSYKLSNSASQLAVRKQK---AQKKXXXXXXXXXXXXXXXXXPHGFAYVKIYQTIGSCPSYNKIDECTEYQMYRTITMILTLLGYSANFFVYFVLNLHFR 1220
            +YVQ  IC+IG ILN++N  +F ++ F S+  Y+L+ ALS+ D+ TL LR+ QGL   R SS           + YI+  + ++PITN TE++S+WLT+ LA+ER + MR ++ ++ Y T +N + LII+I+  + + NLP+F    +      + S        + VIT+   +    ST+Y ++SW R  +VQ +P +FLC+ NVLL T +  HN++F + +  +  S  L+    +G  +D       +    QL  R+QK   AQ+KL ILLI VILLFL   IP  F+Y++I++  G+C        C  Y +YR +T  + L+ Y   FF+YF LN HFR
Sbjct:   33 VYVQPCICLIGFILNIINIYVFQRKTFCSTPAYMLVLALSIADAITLGLRIPQGLTYYRGSSS---VTQTMAIYAYIYATYAEVPITNMTENMSAWLTVCLALERYVAMRHWNFARQYSTRQNTKRLIISIALVSVLFNLPYFMIHRIEFKRFANGS--------MRVITN--ATALRRSTFYTVYSWFRMTIVQIIPLVFLCILNVLLITIVYQHNKKFGKNTEVEQKSNHLSLGT-EGTGADETSLKDTTVGDLQLIQRRQKKRNAQRKLNILLIAVILLFLFGAIPQAFSYIRIFEAFGTCEE--AFHYCPTYVVYRMVTTNIALISYGLTFFLYFFLNQHFR 362
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0R3X330 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS7715 PE=4 SV=1)

HSP 1 Score: 187.578 bits (475), Expect = 5.828e-51
Identity = 125/345 (36.23%), Postives = 197/345 (57.10%), Query Frame = 3 
Query:  201 LYVQIPICVIGCILNMVNFAIFVQQDF-SSATYVLITALSLTDSFTLFLRVGQGLI--RCSSEGWCNNPYFRFFLYIFIVFLDIPITNTTESISSWLTMALAIERCLTMRWYHLSKVYCTIKNARNLIIAISFFATILNLPFFFTLSLNIXXXXXXXXXXXXXVMVPVITSQWTSFAENSTYYLIHSWTRTVLVQYVPFIFLCVSNVLLFTFLRTHNRRFVRASIKKHHSELLA-ANKCKGILSDSYKSYKLSNSAS-QLAVRKQKAQKKXXXXXXXXXXXXXXXXXPHGFAYVKIYQTIGSCPSYNKIDECTEYQMYRTITMILTLLGYSANFFVYFVLNLHFR 1220
            +YVQ  IC+IG ILN++N  +F ++ F S+  Y+L+ ALS+ D+ TL LR+ QGL   R SS           + YI+  + ++PITN TE++S+WLT+ LA+ER + MR ++ ++ YCT +N + LI+ I+  +   NLP+F    +      + S        + V+T+   +    S +Y ++SW R + VQ +P +FLC+ NVLL T +  HN++  + ++ K     L+   +  G    S K   + +S S Q   +K+ AQ+KL ILLI +ILLFL   IP  F+Y++I++  G+C        C  Y +YR +T  + L+ Y   FF+YF LN HFR
Sbjct:   33 VYVQPCICLIGFILNIINSYVFQRKTFCSTPAYMLVLALSIADAITLGLRIPQGLTYYRGSSS---VTQTMAIYAYIYATYAEVPITNMTENMSAWLTVCLALERYVAMRHWNFTRQYCTRQNTKRLIVGIALVSVFFNLPYFMIHRIEFRRFTNGS--------MRVVTN--PTALRRSQFYTVYSWFRMIAVQIIPLVFLCILNVLLITIVYQHNKKIGKNTVMKGKDNRLSLCTEGSGAEEASLKETTVGDSRSTQRRQKKRNAQRKLNILLIAIILLFLFGAIPQAFSYIRIFEAFGTCKE--AFHYCPTYVIYRMVTTNIALISYGLTFFLYFFLNRHFR 362
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A087W1C0 (G protein coupled receptor OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000234800 PE=4 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 1.809e-50
Identity = 129/347 (37.18%), Postives = 200/347 (57.64%), Query Frame = 3 
Query:  201 LYVQIPICVIGCILNMVNFAIFVQQDF-SSATYVLITALSLTDSFTLFLRVGQGLI--RCSSEGWCNNPYFRFFLYIFIVFLDIPITNTTESISSWLTMALAIERCLTMRWYHLSKVYCTIKNARNLIIAISFFATILNLPFFFTLSLNIXXXXXXXXXXXXXVMVPVITSQWTSFAENSTYYLIHSWTRTVLVQYVPFIFLCVSNVLLFTFLRTHNRRFVR-ASIKKHHSELLAANKCKGILSDSYKSYKLSNSASQLAVRKQK---AQKKXXXXXXXXXXXXXXXXXPHGFAYVKIYQTIGSCPSYNKIDECTEYQMYRTITMILTLLGYSANFFVYFVLNLHFR 1220
            +YVQ  IC+IG ILN++N  +F ++ F S+  Y+L+ ALS+ D+ TL LR+ QGL   R SS           + YI+  + ++PITN TE++S+WLT+ LA+ER + MR ++ ++ Y T +  + LII+I+  + + NLP+F    +      + S        + VIT+   +    ST+Y ++SW R  +VQ +P +FLC+ NVLL T +  HN++F +   +++  + L    +  G    S K   + +  SQL  R+QK   AQ+KL ILLI VILLFL   IP  F+Y++I++  G+C        C  Y +YR +T  + L+ Y   FF+YF LN HFR
Sbjct:   41 VYVQPCICLIGFILNIINIYVFQRKTFCSTPAYMLVLALSIADAITLGLRIPQGLTYYRGSSS---VTQTMAIYAYIYATYAEVPITNMTENMSAWLTVCLALERYVAMRHWNFARQYSTRQITKRLIISIALVSVLFNLPYFMIHRIEFKRFANGS--------MRVITN--ATALRRSTFYTVYSWFRMTIVQIIPLVFLCILNVLLITIVYQHNKKFGKNTEVEQKGNHLSLGTEGTGADETSLKDTTVGD--SQLIQRRQKKRNAQRKLNILLIAVILLFLFGAIPQAFSYIRIFEAFGTCEE--AFHYCPTYVVYRMVTTNIALISYGLTFFLYFFLNRHFR 370
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0R3W7Y4 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS6427 PE=4 SV=1)

HSP 1 Score: 185.267 bits (469), Expect = 4.126e-50
Identity = 126/345 (36.52%), Postives = 197/345 (57.10%), Query Frame = 3 
Query:  201 LYVQIPICVIGCILNMVNFAIFVQQDFSSA-TYVLITALSLTDSFTLFLRVGQGLI--RCSSEGWCNNPYFRFFLYIFIVFLDIPITNTTESISSWLTMALAIERCLTMRWYHLSKVYCTIKNARNLIIAISFFATILNLPFFFTLSLNIXXXXXXXXXXXXXVMVPVITSQWTSFAENSTYYLIHSWTRTVLVQYVPFIFLCVSNVLLFTFLRTHNRRFVR-ASIKKHHSELLAANKCKGILSDSYKSYKLSNS-ASQLAVRKQKAQKKXXXXXXXXXXXXXXXXXPHGFAYVKIYQTIGSCPSYNKIDECTEYQMYRTITMILTLLGYSANFFVYFVLNLHFR 1220
            +YVQ  IC +G ILN++N  +F ++ FSS   Y+L+ ALS+ D+ TL LR+ QGL   R SS           + YI+  + ++PITN TE++S+WLT+ LA+ER + MR ++ ++ Y T +N + LII+I+  +   NLP+F    +      + S        + VIT+  T+    S +Y ++SW R   VQ +P +FLC+ NVLL T +  HN++F +   +++  + L    +  G    S K   + +S  +Q   +K+ AQ+KL ILLI +ILLFL   IP  F+Y++I++  G+C        C  Y +YR +T  + L+ Y   FF+YF LN HFR
Sbjct:   33 VYVQPCICFVGFILNIINSYVFQRRTFSSTPAYMLVLALSIADAITLGLRIPQGLTYYRGSSS---VTQTMAIYAYIYATYAEVPITNMTENMSAWLTVCLALERYVAMRHWNFTRQYSTRQNTKRLIISIALVSVFFNLPYFMIHRIEFRRFANGS--------MRVITN--TTALRRSQFYTVYSWFRMTTVQIIPLVFLCILNVLLITIVYQHNKKFGKNTEMEQKGNRLSLGAEGSGAEELSLKDTTMGDSWLTQRRQKKRNAQRKLNILLIAIILLFLFGAIPQAFSYIRIFEAFGTCK--EAFHYCPTYVIYRMVTTNIALISYGLTFFLYFFLNRHFR 362
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000063592.1 (SMESG000063592.1)

HSP 1 Score: 692.575 bits (1786), Expect = 0.000e+0
Identity = 368/369 (99.73%), Postives = 369/369 (100.00%), Query Frame = 3 
Query:  366 RCSSEGWCNNPYFRFFLYIFIVFLDIPITNTTESISSWLTMALAIERCLTMRWYHLSKVYCTIKNARNLIIAISFFATILNLPFFFTLSLNIXXXXXXXXXXXXXVMVPVITSQWTSFAENSTYYLIHSWTRTVLVQYVPFIFLCVSNVLLFTFLRTHNRRFVRASIKKHHSELLAANKCKGILSDSYKSYKLSNSASQLAVRKQKAQKKXXXXXXXXXXXXXXXXXPHGFAYVKIYQTIGSCPSYNKIDECTEYQMYRTITMILTLLGYSANFFVYFVLNLHFRASFFRMFRSKDSDDNTITGDHTEQLLDSKEFQKMTKLYLFPFGQLTYGIAQPRKVKEKPNTSNSEPIQGNKEKVHSFSIRETNL 1472
            RCSSEGWCNNPYFRFFLYIFIVFLDIPITNTTESISSWLTMALAIERCLTMRWYHLSKVYCTIKNARNLIIAISFFATILNLPFFFTLSLNISLNQSWSNASSSNVMVPVITSQWTSFAENSTYYLIHSWTRTVLVQYVPFIFLCVSNVLLFTFLRTHNRRFVRASIKKHHSELLAANKCKGILSDSYKSYKLSNSASQLAVRKQKAQKKLTILLIIVILLFLLFQIPHGFAYVKIYQTIGSCPSYNKIDECTEYQMYRTITMILTLLGYSANFFVYFVLNLHFRASFFRMFRSKDSDDNTITGDHTEQLLDSKEFQKMTKLYLFPFGQLTYGIAQPRKVKEKPNTSNSEPIQGNKE+VHSFSIRETNL
Sbjct:    5 RCSSEGWCNNPYFRFFLYIFIVFLDIPITNTTESISSWLTMALAIERCLTMRWYHLSKVYCTIKNARNLIIAISFFATILNLPFFFTLSLNISLNQSWSNASSSNVMVPVITSQWTSFAENSTYYLIHSWTRTVLVQYVPFIFLCVSNVLLFTFLRTHNRRFVRASIKKHHSELLAANKCKGILSDSYKSYKLSNSASQLAVRKQKAQKKLTILLIIVILLFLLFQIPHGFAYVKIYQTIGSCPSYNKIDECTEYQMYRTITMILTLLGYSANFFVYFVLNLHFRASFFRMFRSKDSDDNTITGDHTEQLLDSKEFQKMTKLYLFPFGQLTYGIAQPRKVKEKPNTSNSEPIQGNKEEVHSFSIRETNL 373
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000035759.1 (SMESG000035759.1)

HSP 1 Score: 246.514 bits (628), Expect = 7.629e-77
Identity = 149/362 (41.16%), Postives = 234/362 (64.64%), Query Frame = 3 
Query:  186 IEVLSLYVQIPICVIGCILNMVNFAIFVQQDFSSATYVLITALSLTDSFTLFLRVGQGLIRCSSEGWCNNPYFRFFLYIFIVFLDIPITNTTESISSWLTMALAIERCLTMRWYHLSKVYCTIKNARNLIIAISFFATILNLPFFFTLSLNIXXXXXXXXXXXXXVMVPVITSQWTSFAENSTYYLIHSWTRTVLVQYVPFIFLCVSNVLLFTFLRTHNRRFVRASIK-----KHHSELLAANKCKGILSDSYK----SYKLSNSASQLAVRKQKAQKKXXXXXXXXXXXXXXXXXPHGFAYVKIYQTIGSCPSYNKIDECTEYQMYRTITMILTLLGYSANFFVYFVLNLHFRASFFRMFR 1244
            I+ L +Y Q PIC+IG ILN +NF +  +   S+ATY+L++AL+ TD+ TL LR  Q  +RC +  +C  P  ++ LYIF+++ ++PITN TE++S W+T+ALA+E  + M+++  S++YCT++N++ LI  ++F +T++NLPFFF ++ ++    +  N S     +PV+ ++   F + S +Y IHSW R  +VQ  P + LCV N LLF+ +   NR +  ++ +     +  S+ L + K + I   S +    S KL    S L  +KQKAQ KLTILLI++I+LFL+ QIPH FAYV I++   +C     + +C ++++YR++TMILTL  YS NFFVY  LN H++ SFF +FR
Sbjct:   19 IDFLLIYFQFPICIIGSILNFINFLVLTRIGISNATYILMSALAFTDASTLLLRTVQTYVRCETLPFCEMPNLKYKLYIFVLYAEVPITNITENLSCWITLALAVEHYIQMKFFTKSRLYCTVENSKKLIFLLTFLSTLVNLPFFFLMTTSVKYETNQRNQS-----IPVVYAKKRQFMD-SIFYPIHSWLRFCIVQMFPLLLLCVFNGLLFSTVYKRNRNYFNSNRQYFKTLRKWSKDLNSIKAQAIEHSSGQCSSPSPKLETRMS-LISKKQKAQYKLTILLIVIIILFLIGQIPHAFAYVGIHRFFSNCKDLTYLKDCPKHELYRSVTMILTLACYSLNFFVYIFLNSHYQNSFFTLFR 373
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000051347.1 (SMESG000051347.1)

HSP 1 Score: 236.498 bits (602), Expect = 1.605e-72
Identity = 141/350 (40.29%), Postives = 218/350 (62.29%), Query Frame = 3 
Query:  189 EVLSLYVQIPICVIGCILNMVNFAIFVQQDFSSATYVLITALSLTDSFTLFLRVGQGLIRCSSEGWCNNPYFRFFLYIFIVFLDIPITNTTESISSWLTMALAIERCLTMRWYHLSKVYCTIKNARNLIIAISFFATILNLPFFFTLSLNIXXXXXXXXXXXXXVMVPVITSQWTSFAENSTYYLIHSWTRTVLVQYVPFIFLCVSNVLLFTFLRTHNRRFVRASIKKHHSELLAANKCKGILSDSYKSYKLSNSASQLAVRKQKAQKKXXXXXXXXXXXXXXXXXPHGFAYVKIYQTI-GSCPSYNKIDECTEYQMYRTITMILTLLGYSANFFVYFVLNLHFRASFFR 1235
            E L +YVQ P+C+IG ILN++NF+IF Q+ FS+A YVL+ A+SL DS TL  R+ + +++C+S  +C NPY +  +Y+F VF+++PITN TESIS+W  +ALA ER + M +Y+LSK Y T+ NA+  ++ ++  + +LN PFF+   + I    + S+      MVPVI S ++S A  S +Y  H+W R  +V+ +P +FLCV N LL   +  H ++  +   K+                  Y + K +N  +Q   RKQ+AQ KLT+LLI+VI+LFL+ QIP  F+Y+K+Y+ +   C +Y  + +C  Y++Y +++ +L +  Y  NFFVY  LN HFR S  +
Sbjct:   12 EFLDVYVQAPLCLIGFILNLINFSIFAQKKFSAAPYVLLMAMSLVDSITLIFRISEAVVKCTSLSFCRNPYLQMVMYLFAVFIEVPITNITESISNWTAVALATERYIAMCYYNLSKQYSTVYNAKLFLLFVTLLSIVLNSPFFYINYVVIEWENTTSHQG----MVPVIYSSFSSLAL-SKFYTFHTWFRPFIVKVIPLLFLCVCNFLLLALVWEHKKKLKQFQSKR----------------SGYLNTKDNNKITQ---RKQRAQNKLTMLLIVVIVLFLIGQIPVSFSYLKVYENLPNRCNAYKYLTDCPNYKVYSSVSHLLMITCYCTNFFVYLALNNHFRNSLMQ 337
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000044466.1 (SMESG000044466.1)

HSP 1 Score: 235.728 bits (600), Expect = 1.809e-72
Identity = 153/348 (43.97%), Postives = 217/348 (62.36%), Query Frame = 3 
Query:  195 LSLYVQIPICVIGCILNMVNFAIFVQQDFSSATYVLITALSLTDSFTLFLRVGQGLIRCSSEGW--CNNPYFRFFLYIFIVFLDIPITNTTESISSWLTMALAIERCLTMRWYHLSKVYCTIKNARNLIIAISFFATILNLPFFFTLSLNIXXXXXXXXXXXXXVMVPVITSQWTSFAENSTYYLIHSWTRTVLVQYVPFIFLCVSNVLLFTFLRTHNRR-FVRASIKKHHSELLAANKCKGILSDSYKSYKLSNSASQLAVRKQKAQKKXXXXXXXXXXXXXXXXXPHGFAYVKIYQTIGSCPSYNKID-ECTEYQMYRTITMILTLLGYSANFFVYFVLNLHFRAS 1226
            LS Y+QIPIC+IG  LN+VN  +F +    S+ Y L+++L+L D+ TL LR  QGL+    E W  C++P  R ++Y+F++FL+ P+TN TE+IS+WLT++LAIER L M+ +  SKVYCT+KN + L   I   A I+NLP+FFT ++ +    +  +          I +  + F+  S  Y +HSW R V+VQ++P + LCV N LLF  +  HNR+ FV      +   + +  + + ++S    S       S    +K +AQKKLTILLI VILLFL+ QIPH FAY+KIY+ +     Y+K D E  EY+M+RT TM+LTLL YS NFF+Y  LN HFR S
Sbjct:   28 LSFYIQIPICLIGFCLNVVNVIVFYKNIHHSSLYFLVSSLALADAVTLILRASQGLL--GKECWPLCSSPQLRMYVYMFVIFLEFPVTNITETISNWLTLSLAIERFLAMKHHSFSKVYCTLKNFKILTSFICIIALIINLPYFFTYNVILKKEYNNESQMYE----KAIYTNESHFSL-SQIYEVHSWIRQVIVQFLPLLLLCVFNFLLFQIVYNHNRKLFVTPRCGTNSIIIDSTKESQRLISKPSLSKHPIKEKSTAIEKKLRAQKKLTILLITVILLFLIGQIPHSFAYIKIYKAVS---KYSKFDPEGFEYRMFRTSTMVLTLLCYSLNFFIYLFLNTHFRHS 365
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000068527.1 (SMESG000068527.1)

HSP 1 Score: 224.172 bits (570), Expect = 3.506e-67
Identity = 147/353 (41.64%), Postives = 218/353 (61.76%), Query Frame = 3 
Query:  189 EVLSLYVQIPICVIGCILNMVNFAIFVQQDFSSATYVLITALSLTDSFTLFLRVGQGLIRCSSEGWCNNPYFRFFLYIFIVFLDIPITNTTESISSWLTMALAIERCLTMRWYHLSKVYCTIKNARNLIIAISFFATILNLPFFFTLSLNIXXXXXXXXXXXXXVMVPVITSQWTSFAENSTYYLIHSWTRTVLVQYVPFIFLCVSNVLLFTFLRTHNRRFVRASIKKHHSELLAANKCKGILSDSYKSYKLSNSASQLAV--RKQKAQKKXXXXXXXXXXXXXXXXXPHGFAYVKIYQTIGSCPSYNKIDECTEYQMYRTITMILTLLGYSANFFVYFVLNLHFRASFFRMF 1241
            E+L +++Q PIC++G  LN++NF++FV++ + +A YVL++ALSL D+ TL L   + + +C    +C  PY +   Y+F +++  P++N TE+ISS +T+ALA ER L MR Y+  K+Y T+KN R  ++ IS F+T+ N PFFFT ++ IS     S        VPV+ S +TS  + S +Y  HSWTR + +Q +P ++LC+ N LL   +  HN+R    SI+  +S          ++ +S     +SN  +Q ++  RKQ AQ KLTILL I+I L+L+ QIP   AY+KIY+   SC ++  I EC  Y MYRT+T I+ L  Y+ NFFVYF LN HFR S    F
Sbjct:   15 ELLVVFLQGPICIVGFGLNIINFSVFVKRKYFAAPYVLLSALSLADAVTLLLYFTETMAKCQHLAFCKQPYLQLSAYVFALYVKNPLSNITETISSIITVALATERYLAMRHYNFRKIYFTVKNTRLFVLLISVFSTLFNTPFFFTTTIKISWENETSTKQK----VPVVYSNYTSLID-SYFYSTHSWTRAIAIQMLPLLYLCICNSLLLALICHHNKRL--DSIRTSYSISTDCRSTSHMVCES----PMSNQTNQSSIRERKQIAQTKLTILLSIIIFLYLIGQIPSALAYIKIYRKFHSCDNFEVIHECPSYDMYRTVTSIMMLASYALNFFVYFFLNSHFRKSLCDCF 356
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
frpr-85.258e-727.85FMRFamide Peptide Receptor family [Source:UniProt... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A068WK466.087e-5235.16G protein coupled receptor OS=Echinococcus granulo... [more]
W6UXS14.929e-5137.28Putative G-protein coupled receptor OS=Echinococcu... [more]
A0A0R3X3305.828e-5136.23G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A087W1C01.809e-5037.18G protein coupled receptor OS=Echinococcus multilo... [more]
A0A0R3W7Y44.126e-5036.52G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000063592.10.000e+099.73SMESG000063592.1[more]
SMESG000035759.17.629e-7741.16SMESG000035759.1[more]
SMESG000051347.11.605e-7240.29SMESG000051347.1[more]
SMESG000044466.11.809e-7243.97SMESG000044466.1[more]
SMESG000068527.13.506e-6741.64SMESG000068527.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015471 ID=SMED30015471|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1483bp
TCTGCCCCCTCTCCATAAATTTTATCGTCATTTAATTCATCTTTATTATC
AAATGGCATTTTGTGGAGTTGTTTTAATTCAATAAAACACCAAGTAAGCA
AATTGTGACAGGTATGGTTGAAAATATCAAGTCTCTCAGTTTCATCGGTG
ATGAGTATTTCCCCCAATCTTCATGGAAAATTGCTATTGAAGTATTGTCC
CTTTACGTACAGATTCCCATCTGTGTCATCGGGTGCATTCTAAACATGGT
CAACTTCGCGATTTTCGTTCAGCAGGATTTCTCCAGCGCTACTTATGTGT
TAATCACTGCTTTATCACTCACGGACAGCTTCACATTGTTTCTTAGAGTT
GGCCAAGGCCTAATTAGATGTTCATCGGAAGGATGGTGCAATAATCCGTA
TTTCCGGTTCTTTCTTTACATTTTTATTGTCTTTCTGGATATCCCAATCA
CAAATACGACAGAAAGCATATCATCATGGCTTACTATGGCATTGGCAATA
GAACGTTGTCTGACAATGAGATGGTACCATTTATCTAAAGTTTATTGTAC
GATTAAAAATGCTCGAAATTTAATAATAGCAATTTCGTTCTTTGCAACGA
TTTTGAACCTACCATTTTTCTTTACATTGTCTTTAAATATATCACTAAAT
CAGTCATGGTCAAATGCATCTTCATCCAATGTAATGGTACCGGTTATTAC
TTCACAATGGACAAGTTTTGCAGAAAATTCAACTTATTATCTCATTCATT
CTTGGACAAGAACTGTCTTGGTCCAATATGTACCCTTCATATTTTTGTGT
GTCTCCAATGTTCTTCTTTTCACATTTCTCAGAACTCATAACCGCCGATT
TGTAAGGGCATCAATAAAAAAACATCATTCAGAGCTGTTGGCTGCAAATA
AATGCAAAGGCATTTTGAGTGACTCTTATAAGTCGTATAAGCTAAGCAAC
AGTGCCTCTCAATTAGCTGTTCGTAAACAAAAAGCCCAGAAAAAACTTAC
AATTTTGCTTATAATTGTAATTTTATTATTTTTACTTTTTCAAATTCCTC
ATGGCTTTGCTTACGTGAAAATCTACCAAACAATCGGATCGTGTCCCTCC
TATAACAAAATTGACGAGTGCACCGAATATCAAATGTATCGAACAATAAC
AATGATTTTAACTTTACTAGGATATTCTGCGAATTTTTTTGTTTATTTTG
TTCTCAATCTTCACTTTCGAGCTTCATTTTTCAGAATGTTTCGATCAAAA
GACAGCGATGACAACACGATCACGGGAGATCACACTGAGCAATTGTTGGA
TTCTAAAGAATTTCAAAAAATGACAAAACTTTATTTGTTTCCATTTGGTC
AACTGACTTATGGAATCGCACAGCCTAGAAAGGTCAAGGAGAAGCCAAAT
ACGTCTAACAGTGAACCTATACAGGGAAATAAAGAAAAGGTGCATTCGTT
TAGCATAAGGGAAACCAATTTGTGACTCTATTC
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protein sequence of SMED30015471-orf-1

>SMED30015471-orf-1 ID=SMED30015471-orf-1|Name=SMED30015471-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=454bp
MVENIKSLSFIGDEYFPQSSWKIAIEVLSLYVQIPICVIGCILNMVNFAI
FVQQDFSSATYVLITALSLTDSFTLFLRVGQGLIRCSSEGWCNNPYFRFF
LYIFIVFLDIPITNTTESISSWLTMALAIERCLTMRWYHLSKVYCTIKNA
RNLIIAISFFATILNLPFFFTLSLNISLNQSWSNASSSNVMVPVITSQWT
SFAENSTYYLIHSWTRTVLVQYVPFIFLCVSNVLLFTFLRTHNRRFVRAS
IKKHHSELLAANKCKGILSDSYKSYKLSNSASQLAVRKQKAQKKLTILLI
IVILLFLLFQIPHGFAYVKIYQTIGSCPSYNKIDECTEYQMYRTITMILT
LLGYSANFFVYFVLNLHFRASFFRMFRSKDSDDNTITGDHTEQLLDSKEF
QKMTKLYLFPFGQLTYGIAQPRKVKEKPNTSNSEPIQGNKEKVHSFSIRE
TNL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR0194277TM_GPCR_serpentine_rcpt_Srw
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: molecular function
TermDefinition
GO:0008528G-protein coupled peptide receptor activity
GO:0004930G-protein coupled receptor activity
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 45..356
e-value: 9.1E-8
score: 31.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 423..453
NoneNo IPR availablePANTHERPTHR46641FAMILY NOT NAMEDcoord: 30..441
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 27..372
e-value: 9.77505E-27
score: 107.334
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 23..379
NoneNo IPR availableTMHMMTMhelixcoord: 295..317
NoneNo IPR availableTMHMMTMhelixcoord: 59..81
NoneNo IPR availableTMHMMTMhelixcoord: 152..174
NoneNo IPR availableTMHMMTMhelixcoord: 217..239
NoneNo IPR availableTMHMMTMhelixcoord: 30..52
NoneNo IPR availableTMHMMTMhelixcoord: 344..366
NoneNo IPR availableTMHMMTMhelixcoord: 96..115
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 43..361
score: 15.778