Sparc

Overview
NameSparc
Smed IDSMED30015470
Length (bp)1014
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Sparc (SMED30015470) t-SNE clustered cells

Violin plots show distribution of expression levels for Sparc (SMED30015470) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Sparc (SMED30015470) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Sparc (SMED30015470) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30015470

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30015470h1SMcG0021139 Contig55376uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30015470h1SMcG0021139 Contig55376newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
cephalic gangliaSMED30015470h1SMcG0021139 dd_Smed_v4_1021_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30015470h1SMcG0021139 dd_Smed_v4_1021_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx musculatureSMED30015470h1SMcG0021139 dd_Smed_v6_1021_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
musculature systemSMED30015470h1SMcG0021139 dd_Smed_v6_1021_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30015470h1SMcG0021139 dd_Smed_v4_1021_0_1dd_Smed_v4PMID:30962434
Cote et al., 2019		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of Sparc vs. Ensembl Celegans
Match: ost-1 (SPARC [Source:UniProtKB/Swiss-Prot;Acc:P34714])

HSP 1 Score: 59.3066 bits (142), Expect = 5.824e-10
Identity = 55/235 (23.40%), Postives = 99/235 (42.13%), Query Frame = 1 
Query:  199 AQDKCANVQCKDGEVCDAGK-----CKCMEQCPEEWNNYPRQLCVDG-KTYRHECDLWRNKCYCTKSDSRCGGAEFGSHKPTDSIVVQYFDACRDLAAQCDWPSEE--ASFHARLAIWFKDLYSQKWSVAXXXXXXXXXXXXXXXXTRASTTTLFNAPTSSPYGVHGGYLSFWFCEMD-KNNNGNLDRTEIALLSQLLNPSTPCLNTFLSKCGS---GTISLSAWNSCFKVPKAE 867
            A++ C + QC  G+ C  GK     C+C+ +CPE   +   ++C +  +T+   CDL+R +C C +    C  A F +      + ++Y   C+ L    D  +EE  A F  R+A W   +  +              L       +     L +   +     H   + + FCE+D K ++ ++   E+  ++  + P   C+  FL  C +   G IS+  W  C  + + E
Sbjct:   49 AKNPCEDHQCGWGKECVVGKKGEPTCECISKCPELDGDPMDKVCANNNQTFTSLCDLYRERCLCKRKSKECSKA-FNA-----KVHLEYLGECKKL----DECTEEHMAQFPERMADWLFQVMKE--------------LKKRRELHKLEWEELLSEAENDDEKKHVYPVIWKFCELDTKPHDKSVSHHELIPITAPVIPMESCIKPFLEGCDANNDGNISIKEWGKCLGLKEGE 259
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BLAST of Sparc vs. UniProt/SwissProt
Match: sp|P34714|SPRC_CAEEL (SPARC OS=Caenorhabditis elegans OX=6239 GN=ost-1 PE=1 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 7.409e-9
Identity = 55/235 (23.40%), Postives = 99/235 (42.13%), Query Frame = 1 
Query:  199 AQDKCANVQCKDGEVCDAGK-----CKCMEQCPEEWNNYPRQLCVDG-KTYRHECDLWRNKCYCTKSDSRCGGAEFGSHKPTDSIVVQYFDACRDLAAQCDWPSEE--ASFHARLAIWFKDLYSQKWSVAXXXXXXXXXXXXXXXXTRASTTTLFNAPTSSPYGVHGGYLSFWFCEMD-KNNNGNLDRTEIALLSQLLNPSTPCLNTFLSKCGS---GTISLSAWNSCFKVPKAE 867
            A++ C + QC  G+ C  GK     C+C+ +CPE   +   ++C +  +T+   CDL+R +C C +    C  A F +      + ++Y   C+ L    D  +EE  A F  R+A W   +  +              L       +     L +   +     H   + + FCE+D K ++ ++   E+  ++  + P   C+  FL  C +   G IS+  W  C  + + E
Sbjct:   49 AKNPCEDHQCGWGKECVVGKKGEPTCECISKCPELDGDPMDKVCANNNQTFTSLCDLYRERCLCKRKSKECSKA-FNA-----KVHLEYLGECKKL----DECTEEHMAQFPERMADWLFQVMKE--------------LKKRRELHKLEWEELLSEAENDDEKKHVYPVIWKFCELDTKPHDKSVSHHELIPITAPVIPMESCIKPFLEGCDANNDGNISIKEWGKCLGLKEGE 259
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BLAST of Sparc vs. TrEMBL
Match: A0A4D6FZN8 (Sparc (Fragment) OS=Schmidtea mediterranea OX=79327 GN=sparc PE=2 SV=1)

HSP 1 Score: 446.047 bits (1146), Expect = 3.660e-156
Identity = 231/232 (99.57%), Postives = 231/232 (99.57%), Query Frame = 1 
Query:  196 AAQDKCANVQCKDGEVCDAGKCKCMEQCPEEWNNYPRQLCVDGKTYRHECDLWRNKCYCTKSDSRCGGAEFGSHKPTDSIVVQYFDACRDLAAQCDWPSEEASFHARLAIWFKDLYSQKWSVAXXXXXXXXXXXXXXXXTRASTTTLFNAPTSSPYGVHGGYLSFWFCEMDKNNNGNLDRTEIALLSQLLNPSTPCLNTFLSKCGSGTISLSAWNSCFKVPKAEEMPCTNFS 891
            AAQDKCANVQCKDGEVCDAGKCKCMEQCPEEWNNYPRQLCVDGKTYRHECDLWRNKCYCTKSDSRCGGAEFGSHKPTDSIVVQYFDACRDLAAQCDW SEEASFHARLAIWFKDLYSQKWSVASGKSDDLSLLSPLSLKTRASTTTLFNAPTSSPYGVHGGYLSFWFCEMDKNNNGNLDRTEIALLSQLLNPSTPCLNTFLSKCGSGTISLSAWNSCFKVPKAEEMPCTNFS
Sbjct:    1 AAQDKCANVQCKDGEVCDAGKCKCMEQCPEEWNNYPRQLCVDGKTYRHECDLWRNKCYCTKSDSRCGGAEFGSHKPTDSIVVQYFDACRDLAAQCDWSSEEASFHARLAIWFKDLYSQKWSVASGKSDDLSLLSPLSLKTRASTTTLFNAPTSSPYGVHGGYLSFWFCEMDKNNNGNLDRTEIALLSQLLNPSTPCLNTFLSKCGSGTISLSAWNSCFKVPKAEEMPCTNFS 232
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BLAST of Sparc vs. TrEMBL
Match: C0KQW5 (SPARC-like protein OS=Dugesia japonica OX=6161 PE=2 SV=1)

HSP 1 Score: 379.022 bits (972), Expect = 3.790e-129
Identity = 195/225 (86.67%), Postives = 204/225 (90.67%), Query Frame = 1 
Query:  217 NVQCKDGEVCDAGKCKCMEQCPEEWNNYPRQLCVDGKTYRHECDLWRNKCYCTKSDSRCGGAEFGSHKPTDSIVVQYFDACRDLAAQCDWPSEEASFHARLAIWFKDLYSQKWSVAXXXXXXXXXXXXXXXXTRASTTTLFNAPTSSPYGVHGGYLSFWFCEMDKNNNGNLDRTEIALLSQLLNPSTPCLNTFLSKCGSGTISLSAWNSCFKVPKAEEMPCTNFS 891
            N +CK GE    GKC  M+QCPEEWN YPR+LCVDGKTYRHECDLWR KCYC KSDSRCGG EFGSHKPTD+IV+QYFD CRDLAAQCDWPSEEASFHARLA+WF+DLYSQKWSVASGKSDD SLLSPLSLKTR+S TTLFNA   SPYGVHGGYLSFWFCEMDKNNNGNLDR+EIALL QLLNPSTPCLNTFLSKCGSGTIS SAWNSCFKVPKAEEMPCT FS
Sbjct:   36 NWKCKLGEFNKEGKCNPMDQCPEEWNEYPRELCVDGKTYRHECDLWREKCYCAKSDSRCGGPEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEEASFHARLAMWFRDLYSQKWSVASGKSDDQSLLSPLSLKTRSSATTLFNATQFSPYGVHGGYLSFWFCEMDKNNNGNLDRSEIALLFQLLNPSTPCLNTFLSKCGSGTISFSAWNSCFKVPKAEEMPCTPFS 260
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BLAST of Sparc vs. TrEMBL
Match: A0A4E0RGJ0 (Secreted Protein Acidic and Cysteine Rich OS=Fasciola hepatica OX=6192 GN=D915_003209 PE=4 SV=1)

HSP 1 Score: 256.529 bits (654), Expect = 4.944e-81
Identity = 126/255 (49.41%), Postives = 175/255 (68.63%), Query Frame = 1 
Query:  130 LIIIGAL-FVVAVTSQKVLETSPAAQDKCANVQCKDGEVCDAGKCKCMEQCPEEWNNYPRQLCVDGKTYRHECDLWRNKCYCTKSDSRCGGAEFGSHKPTDSIVVQYFDACRDLAAQCDWPSEEASFHARLAIWFKDLYSQKWSVAXXXXXXXXXXXXXXXXTRASTTTLFNAPTSSPYGVHGGYLSFWFCEMDKNNNGNLDRTEIALLSQLLNPSTPCLNTFLSKC-GSGTISLSAWNSCFKVPKAEEMPCTNF 888
            + ++G + FVVA      ++      D+CANV+C+ GE C+ GKC+C + CP EW+NY RQLCVDG TYRHECDLWRN+CYC   D RCGG  F +H+  D  V++YFD CRDL+  CDW  +E +F  RL +WF++L  QKWS  SG  DD SLL P+  K RA+TT L +   +S   V+GG +S+WFCEMD+ N G+LD+ +++LL Q+L PS  CL +F+++C  SG+IS   W++CF+VP+ E + C+ F
Sbjct:    7 IFLLGVVGFVVADEYFDRIDEREMKVDECANVKCRTGEWCNQGKCECQDSCPSEWDNYDRQLCVDGTTYRHECDLWRNQCYCRTGDPRCGGEAFNNHRANDESVIKYFDECRDLSGLCDWEQQEDTFALRLGMWFQELLRQKWSSGSGYPDDESLLRPMDSKARAATTKLMS--QTSMERVNGGVISYWFCEMDRRNKGSLDQRDLSLLYQVLLPSNSCLESFMNRCSSSGSISFDQWHNCFEVPQEERIECSRF 259
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BLAST of Sparc vs. TrEMBL
Match: A0A074ZBK8 (SPARC_Ca_bdg domain-containing protein OS=Opisthorchis viverrini OX=6198 GN=T265_07838 PE=4 SV=1)

HSP 1 Score: 250.751 bits (639), Expect = 1.095e-78
Identity = 121/254 (47.64%), Postives = 166/254 (65.35%), Query Frame = 1 
Query:  130 LIIIGALFVVAVTSQKVLETSPAAQDKCANVQCKDGEVCDAGKCKCMEQCPEEWNNYPRQLCVDGKTYRHECDLWRNKCYCTKSDSRCGGAEFGSHKPTDSIVVQYFDACRDLAAQCDWPSEEASFHARLAIWFKDLYSQKWSVAXXXXXXXXXXXXXXXXTRASTTTLFNAPTSSPYGVHGGYLSFWFCEMDKNNNGNLDRTEIALLSQLLNPSTPCLNTFLSKCG-SGTISLSAWNSCFKVPKAEEMPCTNF 888
             ++ G+L  +A   Q   E     +D CANV+C+ GE C+ G+C+C++ C  +WNN+ R+LCVDG TYRHECDLWRN+CYC   DSRCGG  F +H+  D  V++YFD CRDL   CDW  +E SF  RL IWF++L  QKWS  S   DD SLL P+  K RA+T+ L +  T        G +S+WFCE+D+ N G+LD  +++LL Q+L PS  CL TF+++C  SG+IS   W++CF VP+ E + C+ F
Sbjct:    7 FLLFGSL-CIAFGQQDEWERERDVEDACANVKCRQGEWCNQGRCECLDTCASDWNNFDRKLCVDGNTYRHECDLWRNQCYCRTGDSRCGGETFNNHRANDDAVIKYFDECRDLTEMCDWEQQEDSFPIRLGIWFQELLRQKWSSGSDHPDDESLLRPMDSKARAATSKLMSPSTMDR--AESGVISYWFCELDRRNKGSLDERDLSLLYQVLLPSNACLETFMNRCASSGSISFDQWHNCFDVPREERIECSRF 257
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BLAST of Sparc vs. TrEMBL
Match: G7YBC6 (SPARC protein OS=Clonorchis sinensis OX=79923 GN=CLF_104298 PE=4 SV=1)

HSP 1 Score: 248.44 bits (633), Expect = 8.347e-78
Identity = 120/254 (47.24%), Postives = 166/254 (65.35%), Query Frame = 1 
Query:  130 LIIIGALFVVAVTSQKVLETSPAAQDKCANVQCKDGEVCDAGKCKCMEQCPEEWNNYPRQLCVDGKTYRHECDLWRNKCYCTKSDSRCGGAEFGSHKPTDSIVVQYFDACRDLAAQCDWPSEEASFHARLAIWFKDLYSQKWSVAXXXXXXXXXXXXXXXXTRASTTTLFNAPTSSPYGVHGGYLSFWFCEMDKNNNGNLDRTEIALLSQLLNPSTPCLNTFLSKCG-SGTISLSAWNSCFKVPKAEEMPCTNF 888
             ++ G+L  +A   Q   E     +D CANV+C+ GE C+ G+C+C++ C  +WNN+ R+LCVDG TYRHECDLWRN+CYC   DSRCGG  F +H+  D  V++Y+D CRDL   CDW  +E SF  RL IWF++L  QKWS  S   DD SLL P+  K RA+T+ L +  T        G +S+WFCE+D+ N G+LD  +++LL Q+L PS  CL TF+++C  SG+IS   W++CF VP+ E + C+ F
Sbjct:    7 FLLFGSL-CIAFGQQDEWEREREVEDACANVKCRLGEWCNQGRCECLDTCASDWNNFDRKLCVDGNTYRHECDLWRNQCYCRTGDSRCGGETFNNHRANDDAVIKYYDECRDLTEMCDWEQQEDSFPIRLGIWFQELLRQKWSSGSDYPDDESLLRPMDSKARAATSKLMSPTTMDR--AESGVISYWFCELDRRNKGSLDERDLSLLYQVLLPSNACLETFMNRCASSGSISFDQWHNCFDVPREERIECSRF 257
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BLAST of Sparc vs. Planmine SMEST
Match: SMESG000024280.1 (SMESG000024280.1)

HSP 1 Score: 499.59 bits (1285), Expect = 2.028e-180
Identity = 257/257 (100.00%), Postives = 257/257 (100.00%), Query Frame = 1 
Query:  121 MKYLIIIGALFVVAVTSQKVLETSPAAQDKCANVQCKDGEVCDAGKCKCMEQCPEEWNNYPRQLCVDGKTYRHECDLWRNKCYCTKSDSRCGGAEFGSHKPTDSIVVQYFDACRDLAAQCDWPSEEASFHARLAIWFKDLYSQKWSVAXXXXXXXXXXXXXXXXTRASTTTLFNAPTSSPYGVHGGYLSFWFCEMDKNNNGNLDRTEIALLSQLLNPSTPCLNTFLSKCGSGTISLSAWNSCFKVPKAEEMPCTNFS 891
            MKYLIIIGALFVVAVTSQKVLETSPAAQDKCANVQCKDGEVCDAGKCKCMEQCPEEWNNYPRQLCVDGKTYRHECDLWRNKCYCTKSDSRCGGAEFGSHKPTDSIVVQYFDACRDLAAQCDWPSEEASFHARLAIWFKDLYSQKWSVASGKSDDLSLLSPLSLKTRASTTTLFNAPTSSPYGVHGGYLSFWFCEMDKNNNGNLDRTEIALLSQLLNPSTPCLNTFLSKCGSGTISLSAWNSCFKVPKAEEMPCTNFS
Sbjct:    1 MKYLIIIGALFVVAVTSQKVLETSPAAQDKCANVQCKDGEVCDAGKCKCMEQCPEEWNNYPRQLCVDGKTYRHECDLWRNKCYCTKSDSRCGGAEFGSHKPTDSIVVQYFDACRDLAAQCDWPSEEASFHARLAIWFKDLYSQKWSVASGKSDDLSLLSPLSLKTRASTTTLFNAPTSSPYGVHGGYLSFWFCEMDKNNNGNLDRTEIALLSQLLNPSTPCLNTFLSKCGSGTISLSAWNSCFKVPKAEEMPCTNFS 257
The following BLAST results are available for this feature:
BLAST of Sparc vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sparc vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ost-15.824e-1023.40SPARC [Source:UniProtKB/Swiss-Prot;Acc:P34714][more]
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BLAST of Sparc vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sparc vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sparc vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sparc vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sparc vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|P34714|SPRC_CAEEL7.409e-923.40SPARC OS=Caenorhabditis elegans OX=6239 GN=ost-1 P... [more]
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BLAST of Sparc vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4D6FZN83.660e-15699.57Sparc (Fragment) OS=Schmidtea mediterranea OX=7932... [more]
C0KQW53.790e-12986.67SPARC-like protein OS=Dugesia japonica OX=6161 PE=... [more]
A0A4E0RGJ04.944e-8149.41Secreted Protein Acidic and Cysteine Rich OS=Fasci... [more]
A0A074ZBK81.095e-7847.64SPARC_Ca_bdg domain-containing protein OS=Opisthor... [more]
G7YBC68.347e-7847.24SPARC protein OS=Clonorchis sinensis OX=79923 GN=C... [more]
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BLAST of Sparc vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sparc vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sparc vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sparc vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sparc vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sparc vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000024280.12.028e-180100.00SMESG000024280.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015470 ID=SMED30015470|Name=Sparc|organism=Schmidtea mediterranea sexual|type=transcript|length=1014bp
CACCAATTAGGAAGCGGGAAATTTCACAAGCAGTCTCCAATCGATATCAA
CTTAAATGCCATAATTTCTGCATGACATTTTGTAGATTTTTTTAAATTCC
CTCATTCGTATTGTTCCACAATGAAATATTTAATAATAATCGGAGCTTTA
TTTGTGGTTGCAGTTACTAGTCAAAAAGTCTTAGAAACTTCTCCTGCTGC
ACAAGATAAATGTGCCAATGTCCAGTGTAAAGATGGTGAAGTTTGTGATG
CAGGCAAATGCAAATGCATGGAACAATGCCCCGAAGAATGGAATAATTAT
CCCCGACAATTATGTGTAGATGGAAAGACTTATAGACACGAATGTGATCT
GTGGAGAAATAAATGTTATTGCACCAAATCGGATTCTCGGTGCGGAGGGG
CAGAATTCGGCTCTCATAAACCAACTGATTCTATTGTGGTTCAATATTTT
GACGCTTGTCGGGATCTGGCAGCTCAGTGTGATTGGCCAAGTGAGGAAGC
ATCGTTTCATGCTAGATTGGCAATTTGGTTTAAAGACTTGTACAGCCAAA
AATGGTCTGTCGCCAGTGGAAAATCAGATGATCTATCTCTGCTGAGTCCT
TTAAGCCTGAAAACAAGAGCTTCGACAACGACATTGTTCAATGCTCCAAC
TTCATCTCCTTACGGAGTTCATGGAGGCTATTTGAGCTTTTGGTTTTGTG
AAATGGACAAAAATAACAATGGGAATTTGGATCGTACTGAAATTGCGCTC
TTATCTCAGCTTCTCAATCCTTCAACCCCATGTTTGAATACTTTCCTTAG
CAAATGTGGAAGTGGAACCATAAGTTTAAGTGCTTGGAATTCATGCTTTA
AAGTTCCAAAAGCTGAAGAAATGCCGTGCACTAATTTCTCATAAATCTAT
CCAAAAGAACGGTTTTGAACTGCTTGTTTAAACAAATAATTCAACTAGAA
TAAAAATGTTGTAATAAATGACATTAATACTAATGAAATAATTTTTTAAA
ATTTTAATTCTGCT
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protein sequence of SMED30015470-orf-1

>SMED30015470-orf-1 ID=SMED30015470-orf-1|Name=SMED30015470-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=258bp
MKYLIIIGALFVVAVTSQKVLETSPAAQDKCANVQCKDGEVCDAGKCKCM
EQCPEEWNNYPRQLCVDGKTYRHECDLWRNKCYCTKSDSRCGGAEFGSHK
PTDSIVVQYFDACRDLAAQCDWPSEEASFHARLAIWFKDLYSQKWSVASG
KSDDLSLLSPLSLKTRASTTTLFNAPTSSPYGVHGGYLSFWFCEMDKNNN
GNLDRTEIALLSQLLNPSTPCLNTFLSKCGSGTISLSAWNSCFKVPKAEE
MPCTNFS*
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Aliases
The feature 'Sparc' has the following synonyms
Synonym
Dj-SPARC-like
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000428musculature system
PLANA:0000478pharynx musculature
Vocabulary: INTERPRO
TermDefinition
IPR019577SPARC/Testican_Ca-bd-dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: cellular component
TermDefinition
GO:0005578proteinaceous extracellular matrix
Vocabulary: biological process
TermDefinition
GO:0007165signal transduction
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 119..251
e-value: 9.0E-9
score: 37.4
NoneNo IPR availableGENE3DG3DSA:3.30.60.30coord: 26..115
e-value: 1.7E-5
score: 27.1
NoneNo IPR availablePANTHERPTHR13866SPARC OSTEONECTINcoord: 24..246
NoneNo IPR availablePANTHERPTHR13866:SF14BM-40coord: 24..246
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..17
score: 0.75
IPR019577SPARC/Testican, calcium-binding domainPFAMPF10591SPARC_Ca_bdgcoord: 124..242
e-value: 1.7E-8
score: 34.8
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 196..208
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 123..247