SMED30015426
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30015426 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7Note: Hover over icons to view figure legend
Alignments
SMED30015426 aligns in the following genomic locations:
Homology
BLAST of SMED30015426 vs. Planmine SMEST
Match: SMESG000036067.1 (SMESG000036067.1) HSP 1 Score: 236.884 bits (603), Expect = 1.030e-73 Identity = 125/132 (94.70%), Postives = 128/132 (96.97%), Query Frame = 1 Query: 100 QILQSNYNKSGEENENLKFQCDKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKCFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIKSGFAEQIEQLQS 495 +ILQ+NYNKS EENENLKFQ DKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAK FQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNI+S FAEQIEQLQS Sbjct: 442 KILQNNYNKSEEENENLKFQRDKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKYFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIESRFAEQIEQLQS 573 HSP 2 Score: 105.145 bits (261), Expect = 1.405e-26 Identity = 59/109 (54.13%), Postives = 82/109 (75.23%), Query Frame = 1 Query: 166 KYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKCFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIKSGFAEQIEQLQ 492 ++ +I +L+S LKES L +D +DCK +L+ S+ERN QLENE+ VN R +EI + FQ++++DSN SNE++ LEL +SE KF+SLQ +NNNIKSGFA QIEQL+ Sbjct: 563 RFAEQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENELVVNKRTIEEITEDFQLKMEDSNSSNEKMLLELNRSEYKFKSLQIENNNIKSGFASQIEQLE 671 HSP 3 Score: 50.447 bits (119), Expect = 1.261e-7 Identity = 41/121 (33.88%), Postives = 67/121 (55.37%), Query Frame = 1 Query: 163 DKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLE---------------------NEIEVNNRKNKEIAKCFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIKS 462 ++YLA+I +L+S +ES TN+RL + D K +L+RS+ERN +LE N+++ K IAK + Q+ + N S + LK +L + NK + LQ++N++IKS Sbjct: 206 NQYLAQIERLKSGFRESERTNKRLQLNIDQYKTKLERSQERNLKLESANRLKSQMERNRCVNCAELINQLQNEKGKTNRIAKDCEFQLDNLNRSVKNLKSQLNKMGNKCKDLQDENDHIKS 326
BLAST of SMED30015426 vs. Planmine SMEST
Match: SMESG000036067.1 (SMESG000036067.1) HSP 1 Score: 236.884 bits (603), Expect = 1.040e-73 Identity = 125/132 (94.70%), Postives = 128/132 (96.97%), Query Frame = 1 Query: 100 QILQSNYNKSGEENENLKFQCDKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKCFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIKSGFAEQIEQLQS 495 +ILQ+NYNKS EENENLKFQ DKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAK FQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNI+S FAEQIEQLQS Sbjct: 447 KILQNNYNKSEEENENLKFQRDKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKYFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIESRFAEQIEQLQS 578 HSP 2 Score: 105.145 bits (261), Expect = 1.406e-26 Identity = 59/109 (54.13%), Postives = 82/109 (75.23%), Query Frame = 1 Query: 166 KYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKCFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIKSGFAEQIEQLQ 492 ++ +I +L+S LKES L +D +DCK +L+ S+ERN QLENE+ VN R +EI + FQ++++DSN SNE++ LEL +SE KF+SLQ +NNNIKSGFA QIEQL+ Sbjct: 568 RFAEQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENELVVNKRTIEEITEDFQLKMEDSNSSNEKMLLELNRSEYKFKSLQIENNNIKSGFASQIEQLE 676 HSP 3 Score: 50.447 bits (119), Expect = 1.311e-7 Identity = 41/121 (33.88%), Postives = 67/121 (55.37%), Query Frame = 1 Query: 163 DKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLE---------------------NEIEVNNRKNKEIAKCFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIKS 462 ++YLA+I +L+S +ES TN+RL + D K +L+RS+ERN +LE N+++ K IAK + Q+ + N S + LK +L + NK + LQ++N++IKS Sbjct: 211 NQYLAQIERLKSGFRESERTNKRLQLNIDQYKTKLERSQERNLKLESANRLKSQMERNRCVNCAELINQLQNEKGKTNRIAKDCEFQLDNLNRSVKNLKSQLNKMGNKCKDLQDENDHIKS 331
BLAST of SMED30015426 vs. Planmine SMEST
Match: SMESG000036067.1 (SMESG000036067.1) HSP 1 Score: 236.113 bits (601), Expect = 3.630e-73 Identity = 125/132 (94.70%), Postives = 128/132 (96.97%), Query Frame = 1 Query: 100 QILQSNYNKSGEENENLKFQCDKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKCFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIKSGFAEQIEQLQS 495 +ILQ+NYNKS EENENLKFQ DKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAK FQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNI+S FAEQIEQLQS Sbjct: 447 KILQNNYNKSEEENENLKFQRDKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKYFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIESRFAEQIEQLQS 578 HSP 2 Score: 104.76 bits (260), Expect = 1.703e-26 Identity = 59/109 (54.13%), Postives = 82/109 (75.23%), Query Frame = 1 Query: 166 KYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKCFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIKSGFAEQIEQLQ 492 ++ +I +L+S LKES L +D +DCK +L+ S+ERN QLENE+ VN R +EI + FQ++++DSN SNE++ LEL +SE KF+SLQ +NNNIKSGFA QIEQL+ Sbjct: 568 RFAEQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENELVVNKRTIEEITEDFQLKMEDSNSSNEKMLLELNRSEYKFKSLQIENNNIKSGFASQIEQLE 676 HSP 3 Score: 50.0618 bits (118), Expect = 1.567e-7 Identity = 41/121 (33.88%), Postives = 67/121 (55.37%), Query Frame = 1 Query: 163 DKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLE---------------------NEIEVNNRKNKEIAKCFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIKS 462 ++YLA+I +L+S +ES TN+RL + D K +L+RS+ERN +LE N+++ K IAK + Q+ + N S + LK +L + NK + LQ++N++IKS Sbjct: 211 NQYLAQIERLKSGFRESERTNKRLQLNIDQYKTKLERSQERNLKLESANRLKSQMERNRCVNCAELINQLQNEKGKTNRIAKDCEFQLDNLNRSVKNLKSQLNKMGNKCKDLQDENDHIKS 331
BLAST of SMED30015426 vs. Planmine SMEST
Match: SMESG000036067.1 (SMESG000036067.1) HSP 1 Score: 235.728 bits (600), Expect = 4.103e-73 Identity = 125/132 (94.70%), Postives = 128/132 (96.97%), Query Frame = 1 Query: 100 QILQSNYNKSGEENENLKFQCDKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKCFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIKSGFAEQIEQLQS 495 +ILQ+NYNKS EENENLKFQ DKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAK FQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNI+S FAEQIEQLQS Sbjct: 442 KILQNNYNKSEEENENLKFQRDKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKYFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIESRFAEQIEQLQS 573 HSP 2 Score: 104.76 bits (260), Expect = 1.838e-26 Identity = 59/109 (54.13%), Postives = 82/109 (75.23%), Query Frame = 1 Query: 166 KYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKCFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIKSGFAEQIEQLQ 492 ++ +I +L+S LKES L +D +DCK +L+ S+ERN QLENE+ VN R +EI + FQ++++DSN SNE++ LEL +SE KF+SLQ +NNNIKSGFA QIEQL+ Sbjct: 563 RFAEQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENELVVNKRTIEEITEDFQLKMEDSNSSNEKMLLELNRSEYKFKSLQIENNNIKSGFASQIEQLE 671 HSP 3 Score: 50.0618 bits (118), Expect = 1.674e-7 Identity = 41/121 (33.88%), Postives = 67/121 (55.37%), Query Frame = 1 Query: 163 DKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLE---------------------NEIEVNNRKNKEIAKCFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIKS 462 ++YLA+I +L+S +ES TN+RL + D K +L+RS+ERN +LE N+++ K IAK + Q+ + N S + LK +L + NK + LQ++N++IKS Sbjct: 206 NQYLAQIERLKSGFRESERTNKRLQLNIDQYKTKLERSQERNLKLESANRLKSQMERNRCVNCAELINQLQNEKGKTNRIAKDCEFQLDNLNRSVKNLKSQLNKMGNKCKDLQDENDHIKS 326
BLAST of SMED30015426 vs. Planmine SMEST
Match: SMESG000036102.1 (SMESG000036102.1) HSP 1 Score: 165.236 bits (417), Expect = 1.441e-47 Identity = 119/186 (63.98%), Postives = 143/186 (76.88%), Query Frame = 1 Query: 1 CKSLQNENDSNKSQFSXXXXXXXXXXXXXXXXRQILQSNYNKSGEENENLKFQCDKYLAEINKLESVLKESYTTNRRLHKDCDD----------------CKAELQRSEERNS-----QLENEIEVNNRKNKEIAKCFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIKSGFAEQIEQLQS 495 CKSL NENDSNKSQFSG+IGKLELIIKEINEE+QILQ+NYNKSGEENENLKFQC+KYLAEINKLESVLKES TNRRL ++ + K+++ R+ N+ QLENE E + RK +EI K FQ+++++ N S E+LKL+L QSENKF+SLQN+N+NIKSGFA QIEQLQS Sbjct: 377 CKSLLNENDSNKSQFSGQIGKLELIIKEINEEKQILQNNYNKSGEENENLKFQCNKYLAEINKLESVLKESDRTNRRLQQEFEAKSKHSERNSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEIIKDFQLKMENLNRSIEKLKLQLSQSENKFKSLQNENDNIKSGFAAQIEQLQS 562 HSP 2 Score: 108.997 bits (271), Expect = 6.536e-28 Identity = 68/120 (56.67%), Postives = 88/120 (73.33%), Query Frame = 1 Query: 136 ENENLKFQCDKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKCFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIKSGFAEQIEQLQS 495 EN+N+K + A+I +L+S LKES L +D +DCK +L+ S+ERN QLENE+ VN R +EI K FQ++ +DSN S E+LKL+L QSENK +SLQN+ NNIKSGFA QIEQLQS Sbjct: 545 ENDNIK---SGFAAQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENEMAVNKRTIEEITKDFQLKKEDSNRSIEKLKLQLSQSENKCKSLQNE-NNIKSGFAAQIEQLQS 660 HSP 3 Score: 94.7449 bits (234), Expect = 6.312e-23 Identity = 54/108 (50.00%), Postives = 80/108 (74.07%), Query Frame = 1 Query: 169 YLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKCFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIKSGFAEQIEQLQ 492 + A+I +L+S LKES L +D +DCK +L+ S+ERN QLENE+ VN R +EI K FQ++++DSN SNE++ L+L +SE+K +SLQN+N+N KS F+ QI +L+ Sbjct: 651 FAAQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENEMAVNKRTIEEITKDFQLKMEDSNSSNEKMLLQLNRSEDKCKSLQNENDNDKSHFSGQIGKLE 758 HSP 4 Score: 78.9518 bits (193), Expect = 1.729e-17 Identity = 61/150 (40.67%), Postives = 90/150 (60.00%), Query Frame = 1 Query: 139 NENLKFQC-------DKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQL------ENEIEVN-------------NRKNK------EIAKCFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIKSGFAEQIEQLQ 492 NE L+ +C ++YLA+IN+L+S LKES N RL +D DDCK +L S+ RNSQL E++I N N+K+K E+AK +Q+++++S S E+LK EL QS NK +SL N+N++ KS F+ QI +L+ Sbjct: 250 NEKLRSECKILQNETNQYLAQINQLQSALKESKGINGRLQQDFDDCKVKLNHSQRRNSQLESANQYEHQIGTNKCANCAESTRQLQNQKSKVKKKIEELAKYYQLKMENSKSSIEKLKSELNQSVNKCKSLLNENDSNKSQFSGQIGKLE 399 HSP 5 Score: 58.9214 bits (141), Expect = 1.417e-10 Identity = 29/46 (63.04%), Postives = 37/46 (80.43%), Query Frame = 1 Query: 100 QILQSNYNKSGEENENLKFQCDKYLAEINKLESVLKESYTTNRRLH 237 +ILQ+NYNKS +ENENLKFQCDKYLAEINK+E+ S +++H Sbjct: 827 KILQNNYNKSDKENENLKFQCDKYLAEINKMENNRLNSRINTKKIH 872 The following BLAST results are available for this feature:
BLAST of SMED30015426 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30015426 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30015426 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30015426 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30015426 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30015426 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30015426 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30015426 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30015426 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30015426 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30015426 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30015426 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30015426 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30015426 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30015426 ID=SMED30015426|Name=SMED30015426|organism=Schmidtea mediterranea sexual|type=transcript|length=497bpback to top protein sequence of SMED30015426-orf-1 >SMED30015426-orf-1 ID=SMED30015426-orf-1|Name=SMED30015426-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=165bp CKSLQNENDSNKSQFSGKIGKLELIIKEINEERQILQSNYNKSGEENENLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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