Cilia- and flagella-associated protein 20

Overview
NameCilia- and flagella-associated protein 20
Smed IDSMED30015423
Length (bp)1035
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Cilia- and flagella-associated protein 20 (SMED30015423) t-SNE clustered cells

Violin plots show distribution of expression levels for Cilia- and flagella-associated protein 20 (SMED30015423) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Cilia- and flagella-associated protein 20 (SMED30015423) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Cilia- and flagella-associated protein 20 (SMED30015423) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30015423

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30015423SMESG000057288.1 SmedASXL_006986SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30015423SMESG000057288.1 dd_Smed_v4_4843_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30015423SMESG000057288.1 dd_Smed_v4_4843_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30015423SMESG000057288.1 dd_Smed_v4_4843_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30015423SMESG000057288.1 dd_Smed_v4_4843_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30015423SMESG000057288.1 dd_Smed_v4_4843_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30015423SMESG000057288.1 Contig51236newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30015423SMESG000057288.1 Contig51236uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30015423SMESG000057288.1 dd_Smed_v6_4843_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30015423SMESG000057288.1 dd_Smed_v4_4843_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Human
Match: CFAP20 (cilia and flagella associated protein 20 [Source:HGNC Symbol;Acc:HGNC:29523])

HSP 1 Score: 361.688 bits (927), Expect = 1.745e-126
Identity = 167/190 (87.89%), Postives = 185/190 (97.37%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNR 677
            MF+NT+QSGFLSILYSIGSKPLQIW ++V+NGHIKRITDNDIQSLVLEI GTNVSTT+I CP++ +K+LGIKLPFLVMIIKNLKKYFTFEVQ+LDDKN++RRFRASNYQSTTRV+PF+CTMPMRLD+GWNQIQFNL DFTRRAYGTNYIET+RVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQN+
Sbjct:    1 MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEIEGTNVSTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLLDFTRRAYGTNYIETLRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNK 190
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Human
Match: CFAP20 (cilia and flagella associated protein 20 [Source:HGNC Symbol;Acc:HGNC:29523])

HSP 1 Score: 167.548 bits (423), Expect = 2.305e-51
Identity = 78/93 (83.87%), Postives = 89/93 (95.70%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQI 386
            MF+NT+QSGFLSILYSIGSKPLQIW ++V+NGHIKRITDNDIQSLVLEI GTNVSTT+I CP++ +K+LGIKLPFLVMIIKNLKKYFTFEVQ+
Sbjct:    1 MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEIEGTNVSTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFTFEVQM 93
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Human
Match: C3orf67 (chromosome 3 open reading frame 67 [Source:HGNC Symbol;Acc:HGNC:24763])

HSP 1 Score: 66.2402 bits (160), Expect = 2.854e-12
Identity = 50/171 (29.24%), Postives = 82/171 (47.95%), Query Frame = 3 
Query:  108 MFRNTYQSG-FLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVM-IIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRL--DEGWNQIQFNLSDFTRRAY-GTNYIETVRVQIHANCRIRRVY 605
            MF+N YQ G F+ I  + G  P   W        I +  D +++S V  + G++  T  I  P EN++SLG+   FLV+ I   L + F+ E+ I D  NIKRR   S         P    +P+ +   + W  +  +L  FT   + G  +     + + ANC++R+++
Sbjct:    1 MFKNEYQGGAFVEIFSAQGKNPGAKWKILGSPSVIWKEFDKEVKSFVFVLEGSS-QTNKIQLPKENKQSLGLIQRFLVLQIYVPLGQDFSTELLITDLGNIKRRLYLSTVHKELSSTPLHAKIPLFMIKRKIWCNLCIDLVAFTSEIFKGAVFQSLDGIVVSANCKLRKIF 170
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Human
Match: C3orf67 (chromosome 3 open reading frame 67 [Source:HGNC Symbol;Acc:HGNC:24763])

HSP 1 Score: 66.2402 bits (160), Expect = 2.909e-12
Identity = 50/171 (29.24%), Postives = 82/171 (47.95%), Query Frame = 3 
Query:  108 MFRNTYQSG-FLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVM-IIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRL--DEGWNQIQFNLSDFTRRAY-GTNYIETVRVQIHANCRIRRVY 605
            MF+N YQ G F+ I  + G  P   W        I +  D +++S V  + G++  T  I  P EN++SLG+   FLV+ I   L + F+ E+ I D  NIKRR   S         P    +P+ +   + W  +  +L  FT   + G  +     + + ANC++R+++
Sbjct:    1 MFKNEYQGGAFVEIFSAQGKNPGAKWKILGSPSVIWKEFDKEVKSFVFVLEGSS-QTNKIQLPKENKQSLGLIQRFLVLQIYVPLGQDFSTELLITDLGNIKRRLYLSTVHKELSSTPLHAKIPLFMIKRKIWCNLCIDLVAFTSEIFKGAVFQSLDGIVVSANCKLRKIF 170
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Human
Match: C3orf67 (chromosome 3 open reading frame 67 [Source:HGNC Symbol;Acc:HGNC:24763])

HSP 1 Score: 61.6178 bits (148), Expect = 1.025e-9
Identity = 50/171 (29.24%), Postives = 82/171 (47.95%), Query Frame = 3 
Query:  108 MFRNTYQSG-FLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVM-IIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRL--DEGWNQIQFNLSDFTRRAY-GTNYIETVRVQIHANCRIRRVY 605
            MF+N YQ G F+ I  + G  P   W        I +  D +++S V  + G++  T  I  P EN++SLG+   FLV+ I   L + F+ E+ I D  NIKRR   S         P    +P+ +   + W  +  +L  FT   + G  +     + + ANC++R+++
Sbjct:    1 MFKNEYQGGAFVEIFSAQGKNPGAKWKILGSPSVIWKEFDKEVKSFVFVLEGSS-QTNKIQLPKENKQSLGLIQRFLVLQIYVPLGQDFSTELLITDLGNIKRRLYLSTVHKELSSTPLHAKIPLFMIKRKIWCNLCIDLVAFTSEIFKGAVFQSLDGIVVSANCKLRKIF 170
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Celegans
Match: C54C6.6 (Cilia- and flagella-associated protein 20 [Source:UniProtKB/Swiss-Prot;Acc:Q86D25])

HSP 1 Score: 337.035 bits (863), Expect = 6.487e-117
Identity = 148/196 (75.51%), Postives = 177/196 (90.31%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNRNNPDGP 695
            MF NT+QSG LS+LYSIGSKPLQIW  ++KNGH+KRITD +IQSLVLEIMG N+ST FI+CP + +K+LGIKLPF VM++KN+ KYF+FEVQI+DDK IKRRFRASNYQS TRV+PF+CTMPMR+DEGWNQIQFNLSDF +RAYGTNY+ET+R+QIHANCRIRRVYF+DRLY+EDELPAEFKLYLP++ + +   P
Sbjct:    1 MFHNTFQSGLLSVLYSIGSKPLQIWDTQIKNGHVKRITDEEIQSLVLEIMGNNISTAFISCPVDPDKTLGIKLPFFVMVVKNMNKYFSFEVQIIDDKKIKRRFRASNYQSATRVKPFICTMPMRMDEGWNQIQFNLSDFVKRAYGTNYVETLRIQIHANCRIRRVYFADRLYTEDELPAEFKLYLPIRGQLSTQSP 196
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Fly
Match: Bug22 (gene:FBgn0032248 transcript:FBtr0080044)

HSP 1 Score: 358.992 bits (920), Expect = 1.592e-125
Identity = 163/188 (86.70%), Postives = 184/188 (97.87%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQ 671
            MF+NT+QSGFLSILYSIGSKPLQ+W ++V+NGHIKRITDNDIQSLVLEI+GTNVSTTFI CP++ +K+LGIKLPFLVMIIKN+KKYFTFEVQ+LDDKN++RRFRASNYQSTTRV+PF+CTMPMRLDEGWNQIQFNLSDFTRRAYGTNY+ET+RVQIHANCRIRRVYFSDRLYSEDELP EFKL+LP+Q
Sbjct:    1 MFKNTFQSGFLSILYSIGSKPLQLWDKKVRNGHIKRITDNDIQSLVLEIVGTNVSTTFITCPADPKKTLGIKLPFLVMIIKNMKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDEGWNQIQFNLSDFTRRAYGTNYVETLRVQIHANCRIRRVYFSDRLYSEDELPPEFKLFLPIQ 188
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Fly
Match: CG17118 (gene:FBgn0032291 transcript:FBtr0080111)

HSP 1 Score: 269.24 bits (687), Expect = 4.424e-89
Identity = 120/183 (65.57%), Postives = 150/183 (81.97%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKL 656
            M+R  YQ GFL++LYS+GS PL  W    KNG+IKRI D DI+SLVLEIMG+NVSTTFI+CPSE ++ LGIKLPFLV++IKN+ KYF FEV+I DD+   RRFR SN+QS T V+PF   MPM +  GWNQIQFNL+DFTRRAYG+NY+ETV +Q+HAN RIRR+YF+D+LY+E ELP +++L
Sbjct:    1 MYRAAYQKGFLTVLYSVGSSPLNNWSSYTKNGYIKRIYDEDIKSLVLEIMGSNVSTTFIHCPSECKEQLGIKLPFLVLLIKNMHKYFCFEVKIQDDQRFMRRFRVSNFQSKTSVKPFCTAMPMGMSPGWNQIQFNLADFTRRAYGSNYLETVSLQLHANVRIRRIYFADKLYTEAELPNDYRL 183
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Zebrafish
Match: cfap20 (cilia and flagella associated protein 20 [Source:NCBI gene;Acc:393784])

HSP 1 Score: 363.999 bits (933), Expect = 1.705e-127
Identity = 167/190 (87.89%), Postives = 186/190 (97.89%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNR 677
            MF+NT+QSGFLSILYSIGSKPLQIW ++V+NGHIKRITDNDIQSLVLE+ GTNVSTT+I CP++ +K+LGIKLPFLVMIIKNLKKYFTFEVQ+LDDKN++RRFRASNYQSTTRV+PF+CTMPMRLD+GWNQIQFNLSDFTRRAYGTNYIET+RVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQN+
Sbjct:    1 MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEVEGTNVSTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYIETLRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNK 190
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Zebrafish
Match: zgc:162324 (zgc:162324 [Source:ZFIN;Acc:ZDB-GENE-070410-73])

HSP 1 Score: 65.4698 bits (158), Expect = 2.923e-11
Identity = 50/205 (24.39%), Postives = 94/205 (45.85%), Query Frame = 3 
Query:  108 MFRNTYQSGF-LSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIK-NLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLD--EGWNQIQFNLSDFTRRAY-GTNYIETVRVQIHANCRIRRVY--------FSD---RLYSEDELPAEFKLYLPVQN 674
            MFRN YQ G  + I  + G  P+  W    +   I ++ D +++  V  + G N  T  +  P +++ +LG+   FL++ +   L K F+ E  + D++++KRR   S         P    +P+     + W  +  +L   T   + GT ++    + I A C++RR++        F+D   R   ++E+P   +    VQ+
Sbjct:    1 MFRNDYQGGLTVDIFSAQGKDPVAKWKLYGRKPSITKVFDKEVKGFVYSLEG-NSQTHKMQLPKDSKMTLGLIQKFLILQVNIPLGKDFSAEFLVTDEEHLKRRLYLSTVHKELSATPLHARIPLTCHNRDTWCNLCIDLDSLTAVIFRGTRFLSLDGIIISACCKVRRIFTMKNEPADFTDREIRTGPKEEIPRSCQFPCEVQH 204
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Xenopus
Match: tjp2 (tight junction protein 2 [Source:Xenbase;Acc:XB-GENE-480467])

HSP 1 Score: 59.3066 bits (142), Expect = 5.286e-9
Identity = 47/171 (27.49%), Postives = 80/171 (46.78%), Query Frame = 3 
Query:  108 MFRNTYQSG-FLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVM-IIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMR--LDEGWNQIQFNLSDFTRRAY-GTNYIETVRVQIHANCRIRRVY 605
            MF+N YQ G F+ I  + G  P   W    +   I +  D +++S V  + G++  T  +  P E+  +LG+   FL++ +   L + F+ E+ I D  NIKRR   S         P    +P+     + W  +  +L  FT   + G  +     + I ANC++R++Y
Sbjct:    1 MFKNEYQGGAFVEIFSAQGKDPTAKWKLSGRQSAIWKDFDKEVKSFVFVLEGSS-QTNKMQLPKESRHNLGLIQRFLLLQLYVPLGQDFSAELLISDLSNIKRRLYLSTVHKELSSTPLHAKIPLLGLKRKMWCNLCVDLVSFTSGIFKGAVFQSLDGIIISANCKLRKIY 170
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Xenopus
Match: tjp2 (tight junction protein 2 [Source:Xenbase;Acc:XB-GENE-480467])

HSP 1 Score: 58.151 bits (139), Expect = 1.163e-8
Identity = 47/171 (27.49%), Postives = 80/171 (46.78%), Query Frame = 3 
Query:  108 MFRNTYQSG-FLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVM-IIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMR--LDEGWNQIQFNLSDFTRRAY-GTNYIETVRVQIHANCRIRRVY 605
            MF+N YQ G F+ I  + G  P   W    +   I +  D +++S V  + G++  T  +  P E+  +LG+   FL++ +   L + F+ E+ I D  NIKRR   S         P    +P+     + W  +  +L  FT   + G  +     + I ANC++R++Y
Sbjct:    1 MFKNEYQGGAFVEIFSAQGKDPTAKWKLSGRQSAIWKDFDKEVKSFVFVLEGSS-QTNKMQLPKESRHNLGLIQRFLLLQLYVPLGQDFSAELLISDLSNIKRRLYLSTVHKELSSTPLHAKIPLLGLKRKMWCNLCVDLVSFTSGIFKGAVFQSLDGIIISANCKLRKIY 170
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Mouse
Match: Cfap20 (cilia and flagella associated protein 20 [Source:MGI Symbol;Acc:MGI:107428])

HSP 1 Score: 364.385 bits (934), Expect = 1.152e-127
Identity = 168/190 (88.42%), Postives = 186/190 (97.89%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNR 677
            MF+NT+QSGFLSILYSIGSKPLQIW ++V+NGHIKRITDNDIQSLVLEI GTNVSTT+I CP++ +K+LGIKLPFLVMIIKNLKKYFTFEVQ+LDDKN++RRFRASNYQSTTRV+PF+CTMPMRLD+GWNQIQFNLSDFTRRAYGTNYIET+RVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQN+
Sbjct:    1 MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEIEGTNVSTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYIETLRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNK 190
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Mouse
Match: Cfap20 (cilia and flagella associated protein 20 [Source:MGI Symbol;Acc:MGI:107428])

HSP 1 Score: 291.967 bits (746), Expect = 3.335e-99
Identity = 134/156 (85.90%), Postives = 152/156 (97.44%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQI 575
            MF+NT+QSGFLSILYSIGSKPLQIW ++V+NGHIKRITDNDIQSLVLEI GTNVSTT+I CP++ +K+LGIKLPFLVMIIKNLKKYFTFEVQ+LDDKN++RRFRASNYQSTTRV+PF+CTMPMRLD+GWNQIQFNLSDFTRRAYGTNYIET+RVQ+
Sbjct:    1 MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEIEGTNVSTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYIETLRVQV 156
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Mouse
Match: Cfap20 (cilia and flagella associated protein 20 [Source:MGI Symbol;Acc:MGI:107428])

HSP 1 Score: 185.652 bits (470), Expect = 1.211e-58
Identity = 86/103 (83.50%), Postives = 99/103 (96.12%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRF 416
            MF+NT+QSGFLSILYSIGSKPLQIW ++V+NGHIKRITDNDIQSLVLEI GTNVSTT+I CP++ +K+LGIKLPFLVMIIKNLKKYFTFEVQ+LDDKN++RRF
Sbjct:    1 MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEIEGTNVSTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRRF 103
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Mouse
Match: Cfap20 (cilia and flagella associated protein 20 [Source:MGI Symbol;Acc:MGI:107428])

HSP 1 Score: 57.7658 bits (138), Expect = 1.094e-10
Identity = 24/34 (70.59%), Postives = 29/34 (85.29%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHI 209
            MF+NT+QSGFLSILYSIGSKPLQIW ++    H+
Sbjct:    1 MFKNTFQSGFLSILYSIGSKPLQIWDKKYHIHHV 34
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Mouse
Match: Cfap20 (cilia and flagella associated protein 20 [Source:MGI Symbol;Acc:MGI:107428])

HSP 1 Score: 53.5286 bits (127), Expect = 2.093e-9
Identity = 25/32 (78.12%), Postives = 28/32 (87.50%), Query Frame = 3 
Query:  186 REVKNGHIKRITDNDIQSLVLEIMGTNVSTTF 281
            ++V+NGHIKRITDNDIQSLVLEI GTNV   F
Sbjct:    4 QQVRNGHIKRITDNDIQSLVLEIEGTNVRQGF 35
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. UniProt/SwissProt
Match: sp|Q8BTU1|CFA20_MOUSE (Cilia- and flagella-associated protein 20 OS=Mus musculus OX=10090 GN=Cfap20 PE=1 SV=1)

HSP 1 Score: 364.385 bits (934), Expect = 8.061e-127
Identity = 168/190 (88.42%), Postives = 186/190 (97.89%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNR 677
            MF+NT+QSGFLSILYSIGSKPLQIW ++V+NGHIKRITDNDIQSLVLEI GTNVSTT+I CP++ +K+LGIKLPFLVMIIKNLKKYFTFEVQ+LDDKN++RRFRASNYQSTTRV+PF+CTMPMRLD+GWNQIQFNLSDFTRRAYGTNYIET+RVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQN+
Sbjct:    1 MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEIEGTNVSTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYIETLRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNK 190
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. UniProt/SwissProt
Match: sp|Q6B857|CFA20_BOVIN (Cilia- and flagella-associated protein 20 OS=Bos taurus OX=9913 GN=CFAP20 PE=2 SV=1)

HSP 1 Score: 364.385 bits (934), Expect = 8.061e-127
Identity = 168/190 (88.42%), Postives = 186/190 (97.89%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNR 677
            MF+NT+QSGFLSILYSIGSKPLQIW ++V+NGHIKRITDNDIQSLVLEI GTNVSTT+I CP++ +K+LGIKLPFLVMIIKNLKKYFTFEVQ+LDDKN++RRFRASNYQSTTRV+PF+CTMPMRLD+GWNQIQFNLSDFTRRAYGTNYIET+RVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQN+
Sbjct:    1 MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEIEGTNVSTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYIETLRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNK 190
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. UniProt/SwissProt
Match: sp|Q6GL74|CFA20_XENTR (Cilia- and flagella-associated protein 20 OS=Xenopus tropicalis OX=8364 GN=cfap20 PE=2 SV=1)

HSP 1 Score: 364.385 bits (934), Expect = 9.499e-127
Identity = 167/190 (87.89%), Postives = 186/190 (97.89%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNR 677
            MF+NT+QSGFLSILYSIGSKPLQIW ++V+NGHIKRITDNDIQSLVLE+ GTNVSTT+I CP++ +K+LGIKLPFLVMIIKNLKKYFTFEVQ+LDDKN++RRFRASNYQSTTRV+PF+CTMPMRLD+GWNQIQFNLSDFTRRAYGTNYIET+RVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQN+
Sbjct:    1 MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEVEGTNVSTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYIETLRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNK 190
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. UniProt/SwissProt
Match: sp|Q6GPY6|CFA20_XENLA (Cilia- and flagella-associated protein 20 OS=Xenopus laevis OX=8355 GN=cfap20 PE=2 SV=1)

HSP 1 Score: 364.385 bits (934), Expect = 9.499e-127
Identity = 167/190 (87.89%), Postives = 186/190 (97.89%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNR 677
            MF+NT+QSGFLSILYSIGSKPLQIW ++V+NGHIKRITDNDIQSLVLE+ GTNVSTT+I CP++ +K+LGIKLPFLVMIIKNLKKYFTFEVQ+LDDKN++RRFRASNYQSTTRV+PF+CTMPMRLD+GWNQIQFNLSDFTRRAYGTNYIET+RVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQN+
Sbjct:    1 MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEVEGTNVSTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYIETLRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNK 190
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. UniProt/SwissProt
Match: sp|Q5ZHP3|CFA20_CHICK (Cilia- and flagella-associated protein 20 OS=Gallus gallus OX=9031 GN=CFAP20 PE=2 SV=1)

HSP 1 Score: 364.385 bits (934), Expect = 9.499e-127
Identity = 167/190 (87.89%), Postives = 186/190 (97.89%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNR 677
            MF+NT+QSGFLS+LYSIGSKPLQIW ++V+NGHIKRITDNDIQSLVLEI GTNVSTT+I CP++ +K+LGIKLPFLVMIIKNLKKYFTFEVQ+LDDKN++RRFRASNYQSTTRV+PF+CTMPMRLD+GWNQIQFNLSDFTRRAYGTNYIET+RVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQN+
Sbjct:    1 MFKNTFQSGFLSVLYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEIEGTNVSTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYIETLRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNK 190
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. TrEMBL
Match: A0A2T7NIS1 (DUF667 domain-containing protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_19239 PE=4 SV=1)

HSP 1 Score: 369.392 bits (947), Expect = 3.206e-126
Identity = 170/197 (86.29%), Postives = 190/197 (96.45%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNRNNPDGPQ 698
            MF+NT+QSGFLSILYSIGSKPLQIW ++V+NGHIKRITDNDIQSLVLEI+GTNVSTT+I CP++ +K+LGIKLPFLVMIIKNLKKYFTFEVQ+LDDKN++RRFRASNYQSTTRV+PF+CTMPMRLD+GWNQIQFNLSDFTRRAYGTNYIET+RVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQN+    GP+
Sbjct:    1 MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEIVGTNVSTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYIETLRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNKAAAGGPK 197
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. TrEMBL
Match: A0A1I8HHM7 (DUF667 domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 366.696 bits (940), Expect = 3.707e-125
Identity = 170/197 (86.29%), Postives = 186/197 (94.42%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNRNNPDGPQ 698
            MF++T+QSGFLS+LYSIGSKPL IW R VKNGHIKRITDNDIQSLVLEIMGTNVSTT+I CP E+ ++LGIKLPFLVMIIKNLKKYFTFEVQILDDKN++RRFRASNYQSTTRV+PF+CTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIET R+QIHANCRIRRVYFSDRLY+EDELPAEFKLYLPVQ + +  GPQ
Sbjct:    1 MFKDTFQSGFLSVLYSIGSKPLTIWNRNVKNGHIKRITDNDIQSLVLEIMGTNVSTTYITCPKESGRTLGIKLPFLVMIIKNLKKYFTFEVQILDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETQRIQIHANCRIRRVYFSDRLYTEDELPAEFKLYLPVQAKADKPGPQ 197
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. TrEMBL
Match: V4A7R8 (DUF667 domain-containing protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_191567 PE=4 SV=1)

HSP 1 Score: 366.311 bits (939), Expect = 4.081e-125
Identity = 170/190 (89.47%), Postives = 186/190 (97.89%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNR 677
            MF+NT+QSGFLSILYSIGSKPLQIW ++V+NGHIKRITDNDIQSLVLEI+GTNVSTTFI CP+E +K+LGIKLPFLVMIIKNLKKYFTFEV +LDDKN+KRRFRASNYQSTTRV+PF+CTMPMRLD+GWNQIQFNLSDFTRRAYGTNYIET+RVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQN+
Sbjct:    1 MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEIVGTNVSTTFIQCPTEPKKTLGIKLPFLVMIIKNLKKYFTFEVTVLDDKNVKRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYIETLRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNK 190
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. TrEMBL
Match: A0A2B4SY99 (UPF0468 protein C16orf80-like OS=Stylophora pistillata OX=50429 GN=AWC38_SpisGene1815 PE=4 SV=1)

HSP 1 Score: 366.311 bits (939), Expect = 4.890e-125
Identity = 167/190 (87.89%), Postives = 187/190 (98.42%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNR 677
            MF+NT+QSGFLSILYSIGSKPLQIW ++V+NGHIKRITDNDIQSLVLE++GTNVSTT+I CP++ +K+LGIKLPFLVMIIKNLKKYFTFEVQ+LDDKN++RRFRASNYQSTTRV+PF+CTMPMRLD+GWNQIQFNLSDFTRRAYGTNYIET+RVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQN+
Sbjct:    1 MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEVVGTNVSTTYITCPADAKKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYIETLRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNK 190
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. TrEMBL
Match: K1R2T7 (UPF0468 protein C16orf80-like protein OS=Crassostrea gigas OX=29159 GN=CGI_10009679 PE=4 SV=1)

HSP 1 Score: 365.925 bits (938), Expect = 6.289e-125
Identity = 168/190 (88.42%), Postives = 187/190 (98.42%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNR 677
            MF+NT+QSGFLSILYSIGSKPLQIW ++V+NGHIKRITDNDIQSLVLEI+GTNVSTT+I CP++ +K+LGIKLPFLVMIIKNLKKYFTFEVQ+LDDKN++RRFRASNYQSTTRV+PF+CTMPMRLD+GWNQIQFNLSDFTRRAYGTNYIET+RVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQN+
Sbjct:    1 MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEIVGTNVSTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYIETLRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNK 190
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Cavefish
Match: cfap20 (cilia and flagella associated protein 20 [Source:NCBI gene;Acc:103039883])

HSP 1 Score: 364.385 bits (934), Expect = 3.392e-127
Identity = 172/214 (80.37%), Postives = 195/214 (91.12%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNRNNPDGPQ*CWLYTVPICSNLKYFC 749
            MF+NT+QSGFLSILYSIGSKPLQIW ++V+NGHIKRITDNDIQSLVLE+ GTNVSTT+I CP++ +K+LGIKLPFLVMIIKNLKKYFTFEVQ+LDDKN++RRFRASNYQSTTRV+PF+CTMPMRLD+GWNQIQFNLSDFTRRAYGTNYIET+RVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQN+      Q  + Y   +C+N +  C
Sbjct:    1 MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEVEGTNVSTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYIETLRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNKAKVSSHQHRYFY---LCTNCRPVC 211
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Cavefish
Match: zgc:162324 (chromosome 13 C3orf67 homolog [Source:NCBI gene;Acc:103033090])

HSP 1 Score: 53.5286 bits (127), Expect = 1.707e-7
Identity = 40/171 (23.39%), Postives = 77/171 (45.03%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSI-GSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIK-NLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMR--LDEGWNQIQFNLSDFTRRAY-GTNYIETVRVQIHANCRIRRVY 605
            MFRN YQ G +  ++S  G  P+  W        I ++ D +++  V  + G++  T  +  P + +  L +   +LV+ +   L K F+ E+ + D  ++KRR   S         P    +P+       W  +  +L  FT   + G  ++    + I A C+++R++
Sbjct:    1 MFRNEYQGGAIVEIFSAQGKDPVAKWKVSGGLSSISKVLDKEVKGFVYSLEGSS-QTHRMQLPKDGKMPLVLIQRYLVLQVNVPLGKDFSTELVVTDQGHLKRRLYFSTVHKEFSATPLHARVPLTGLRRNTWCNLCIDLGSFTAELFRGAAFLSLDGIIISACCKVKRIF 170
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Cavefish
Match: zgc:162324 (chromosome 13 C3orf67 homolog [Source:NCBI gene;Acc:103033090])

HSP 1 Score: 53.5286 bits (127), Expect = 2.038e-7
Identity = 40/171 (23.39%), Postives = 77/171 (45.03%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSI-GSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIK-NLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMR--LDEGWNQIQFNLSDFTRRAY-GTNYIETVRVQIHANCRIRRVY 605
            MFRN YQ G +  ++S  G  P+  W        I ++ D +++  V  + G++  T  +  P + +  L +   +LV+ +   L K F+ E+ + D  ++KRR   S         P    +P+       W  +  +L  FT   + G  ++    + I A C+++R++
Sbjct:    1 MFRNEYQGGAIVEIFSAQGKDPVAKWKVSGGLSSISKVLDKEVKGFVYSLEGSS-QTHRMQLPKDGKMPLVLIQRYLVLQVNVPLGKDFSTELVVTDQGHLKRRLYFSTVHKEFSATPLHARVPLTGLRRNTWCNLCIDLGSFTAELFRGAAFLSLDGIIISACCKVKRIF 170
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000785.1 (pep scaffold:Pmarinus_7.0:GL477446:372075:392529:1 gene:ENSPMAG00000000714.1 transcript:ENSPMAT00000000785.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 363.229 bits (931), Expect = 4.968e-128
Identity = 166/190 (87.37%), Postives = 186/190 (97.89%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNR 677
            MF+NT+QSGFLSILYSIGSKPLQIW ++V+NGHIKRITDNDIQSLVLE+ GTNVSTT+I CP++ +K+LGIKLPFLVMIIKNLKKYFTFEVQ+LDDKN++RRFRASNYQSTTRV+PF+CTMPMRLD+GWNQIQFNLSDFTRRAYGTNYIET+RVQIHANCR+RRVYFSDRLYSEDELPAEFKLYLPVQN+
Sbjct:    1 MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEVEGTNVSTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYIETLRVQIHANCRVRRVYFSDRLYSEDELPAEFKLYLPVQNK 190
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002633.1 (pep scaffold:Pmarinus_7.0:GL481839:16843:20127:-1 gene:ENSPMAG00000002397.1 transcript:ENSPMAT00000002633.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 315.849 bits (808), Expect = 1.103e-109
Identity = 145/164 (88.41%), Postives = 161/164 (98.17%), Query Frame = 3 
Query:  189 EVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNRN 680
            +V+NGHIKRITDNDIQSLVLEI GTNVSTT+I CP++ +K+LGIKLPFL+MIIKNLKKYFTFEVQ+LDDKN++RRFRASNYQSTTRV+PF+CTMPMRLD+GWNQIQFNLSDFTRRAYGTNYIET+RVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQN+N
Sbjct:    1 QVRNGHIKRITDNDIQSLVLEIEGTNVSTTYITCPADPKKTLGIKLPFLIMIIKNLKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYIETLRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNKN 164
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Nematostella
Match: EDO35965 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJZ7])

HSP 1 Score: 365.155 bits (936), Expect = 1.631e-128
Identity = 169/190 (88.95%), Postives = 186/190 (97.89%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNR 677
            MF+NT+QSGFLSILYSIGSKPLQIW ++V+NGHIKRITDNDIQSLVLEI+GTNVSTT+I CPSE +K+LGIKLPFLVMIIKNLKKYFTFEVQ+LDDKN+KRRFRASNYQSTTRV+PF+CTMPMRLD+GWNQIQFNLSDFTRRAYGTNYIET+RVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLP+ N+
Sbjct:    1 MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEIVGTNVSTTYITCPSEPQKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVKRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYIETLRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPMNNK 190
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Nematostella
Match: EDO48705 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RIH0])

HSP 1 Score: 48.1358 bits (113), Expect = 3.573e-6
Identity = 40/161 (24.84%), Postives = 75/161 (46.58%), Query Frame = 3 
Query:  135 FLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVM-IIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMR-LDEG-WNQIQFNLSDFTRRAY-GTNYIETVRVQIHANCRIRRVY 605
            F+ +  + G  P+  W         K+I D D++S V  + G + +TT ++ P + ++SL +   +L++ +   L   F+FE+ I D  N KRR   S+      V P     P+  L  G W  +  +L+      + G  +     + + ANC +R+++
Sbjct:  166 FVEVFSAQGRDPVSKWRLCGNASTAKKIFDRDVKSYVFILEGES-TTTKMSIPKDEKQSLFLIQKYLILQLFVPLGHSFSFELGITDMGNNKRRIFMSSSHREVTVTPLHARFPLSILRLGVWLNLCLDLNSLVGETFKGQAFKVLESLTVSANCHLRKIF 325
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Medaka
Match: cfap20 (cilia and flagella associated protein 20 [Source:NCBI gene;Acc:101155160])

HSP 1 Score: 347.821 bits (891), Expect = 3.460e-121
Identity = 158/190 (83.16%), Postives = 180/190 (94.74%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNR 677
            MF+NT+QSGFLSILYSIGSKPLQIW ++V+NGHIK++ DNDI S VLEI G NV TT+I CP++ +K+LGIKLPFLVMIIKNLKKYF+FEVQ+LDDKN++RRFRASNYQSTTRV+PF+CTMPMRLD+GWNQIQFNLSDFTRRAYGTNY ET+RVQIHANCRIRRVYFSDRLY+EDELPAEFKLYLPVQN+
Sbjct:    1 MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKKVLDNDIHSHVLEIEGANVCTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFSFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYSETLRVQIHANCRIRRVYFSDRLYAEDELPAEFKLYLPVQNQ 190
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Medaka
Match: ENSORLT00000009678.2 (cilia- and flagella-associated protein 20 [Source:NCBI gene;Acc:101158233])

HSP 1 Score: 324.709 bits (831), Expect = 4.346e-112
Identity = 148/188 (78.72%), Postives = 171/188 (90.96%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQ 671
            MF+N++QSG  SIL+SIGSKPLQIW  +V+NGHIKR+ D+DI+S V+EI G NV TT+I CP++ +K+LGIKLPFLVMIIKN KKYF+FEVQ+LDDKN++RRFRASNYQSTT+V  F CTMPMRLD+GWNQIQFNLSDFTRRAYGTNY ET+RVQIHANCRIRRVYFSDRLYSEDELPAEFKL LP+Q
Sbjct:    1 MFKNSFQSGLFSILFSIGSKPLQIWDEKVRNGHIKRVLDDDIRSHVMEIEGVNVCTTYITCPADPKKTLGIKLPFLVMIIKNQKKYFSFEVQVLDDKNVRRRFRASNYQSTTKVNSFSCTMPMRLDDGWNQIQFNLSDFTRRAYGTNYSETLRVQIHANCRIRRVYFSDRLYSEDELPAEFKLVLPIQ 188
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Medaka
Match: ENSORLT00000033906.1 (pep primary_assembly:ASM223467v1:1:15541467:15544163:1 gene:ENSORLG00000023233.1 transcript:ENSORLT00000033906.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 133.265 bits (334), Expect = 3.620e-37
Identity = 76/191 (39.79%), Postives = 113/191 (59.16%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHA-----NCRIRRVYFSDRLYSEDELPAEFKLYLP 665
            + R  YQ GF S+L ++G  P Q W  +V     KR+    + SL+      + STT+I CP++  K  GIK PFLV+I+K   + F+FEVQI D++N++   + S      +V  F   +P + D GW ++ F+L + T  ++G++YIET+R+QI          IRRVYF+DR YSEDE+  +FKL LP
Sbjct:    1 LIRQAYQVGFFSVLSAVGGHPFQNWQVQVD----KRV----VASLMCFQTPPSCSTTYITCPTDPTKIPGIKSPFLVLILKT-TEMFSFEVQISDNRNVRWWLKWSTIHKEKKVDHFRANLPFQGD-GWRRLVFDLQNITNTSFGSSYIETLRIQICGREASEGIWIRRVYFTDREYSEDEIFKDFKLPLP 181
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Medaka
Match: ENSORLT00000041219.1 (pep primary_assembly:ASM223467v1:1:15541467:15544163:1 gene:ENSORLG00000023233.1 transcript:ENSORLT00000041219.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 130.954 bits (328), Expect = 3.969e-36
Identity = 78/200 (39.00%), Postives = 117/200 (58.50%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGH-IKRI----TDNDIQSLVLEIM----GTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHA-----NCRIRRVYFSDRLYSEDELPAEFKLYLP 665
            + R  YQ GF S+L ++G  P Q       N H IK++    TD + + +V  +M      + STT+I CP++  K  GIK PFLV+I+K   + F+FEVQI D++N++   + S      +V  F   +P + D GW ++ F+L + T  ++G++YIET+R+QI          IRRVYF+DR YSEDE+  +FKL LP
Sbjct:    1 LIRQAYQVGFFSVLSAVGGHPFQ-------NCHSIKKVEINATDAEHKRVVASLMCFQTPPSCSTTYITCPTDPTKIPGIKSPFLVLILKT-TEMFSFEVQISDNRNVRWWLKWSTIHKEKKVDHFRANLPFQGD-GWRRLVFDLQNITNTSFGSSYIETLRIQICGREASEGIWIRRVYFTDREYSEDEIFKDFKLPLP 191
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Medaka
Match: ENSORLT00000038818.1 (pep primary_assembly:ASM223467v1:1:28238078:28241051:-1 gene:ENSORLG00000025180.1 transcript:ENSORLT00000038818.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 100.523 bits (249), Expect = 7.970e-26
Identity = 55/107 (51.40%), Postives = 68/107 (63.55%), Query Frame = 3 
Query:  306 KSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYS 626
            K+ G K PFLVMI  N              KN++R FR S  QS T+           L++GWNQIQFNLSD T+ A+GTNY+ET+RVQIH N RIRRV+F++RLYS
Sbjct:   26 KTWGCKSPFLVMITYN--------------KNVRRWFRMSKNQSNTK-----------LEDGWNQIQFNLSDLTKTAFGTNYMETLRVQIHVNRRIRRVFFTERLYS 107
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Planmine SMEST
Match: SMESG000057288.1 (SMESG000057288.1)

HSP 1 Score: 410.223 bits (1053), Expect = 5.851e-146
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNRNNPDGPQ 698
            MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNRNNPDGPQ
Sbjct:    1 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNRNNPDGPQ 197
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Planmine SMEST
Match: SMESG000081781.1 (SMESG000081781.1)

HSP 1 Score: 375.17 bits (962), Expect = 5.653e-132
Identity = 173/191 (90.58%), Postives = 187/191 (97.91%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNRN 680
            MFRNTYQSGFLSILYSIGSKPLQIWG+EVKNGH+KRITDNDIQSLVLE+MGTNVSTTF++CP   +K LGIKLPFLVMI+KNLKKYFTFE+QILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNY+ET+R+QIHANCRIRRVYFSDRLYSEDELPAEFKLYLP+QN++
Sbjct:    1 MFRNTYQSGFLSILYSIGSKPLQIWGKEVKNGHVKRITDNDIQSLVLEVMGTNVSTTFLSCPESPDKVLGIKLPFLVMIVKNLKKYFTFEIQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYVETLRIQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPIQNKS 191
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Planmine SMEST
Match: SMESG000059817.1 (SMESG000059817.1)

HSP 1 Score: 335.88 bits (860), Expect = 1.050e-116
Identity = 145/192 (75.52%), Postives = 182/192 (94.79%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNRNN 683
            MFRNT+QSGFLSIL+SIG+ PLQIW ++V+NGHIKRI+DNDIQSLV+E+MGTN+STTFI CP + + +LGIKLP++++I+KNLKKYF+FEVQILDDKN++RRFRASNYQSTTRV+PF+CTMP+RLDEGWNQI FNL+DF +RAYGTNY+ET+R+Q+HANCRIRR+YFSD+LYSE+ELP EFKLYLP+Q +++
Sbjct:    1 MFRNTFQSGFLSILFSIGTNPLQIWDKKVRNGHIKRISDNDIQSLVIELMGTNISTTFITCPCDPKVTLGIKLPYMILIVKNLKKYFSFEVQILDDKNVRRRFRASNYQSTTRVKPFICTMPLRLDEGWNQIHFNLADFNKRAYGTNYVETLRIQVHANCRIRRIYFSDKLYSEEELPPEFKLYLPIQQKSS 192
[Close]
BLAST of Cilia- and flagella-associated protein 20 vs. Planmine SMEST
Match: SMESG000026833.1 (SMESG000026833.1)

HSP 1 Score: 196.438 bits (498), Expect = 5.503e-62
Identity = 87/189 (46.03%), Postives = 126/189 (66.67%), Query Frame = 3 
Query:  108 MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIMGTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIKRRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIETVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQN 674
            M+ N +Q   LSI YS+GSKPL+ W   V +G +KRITDN I+SLVLE   TN++  +I+CP +  + L I++P+ +M+ KN  K F+FE++I DDK   RRFR S + STT  Q F  ++P+ +  GWN I F++    +  YG  + +  R++IHANC +RR+YFSDRLY + EL  EFKL +P ++
Sbjct:    1 MYSNAFQKRQLSIFYSVGSKPLRNWSIFVNSGTLKRITDNVIKSLVLEFQATNIANNYISCPKDPNEILAIRMPYFIMLFKNTNKAFSFELEIGDDKGFNRRFRVSTFNSTTVAQTFAASLPLNVSPGWNTIMFDMRRLMQSLYGAKFYQVTRIKIHANCSLRRIYFSDRLYQDTELKDEFKLIVPTKS 189
The following BLAST results are available for this feature:
BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CFAP201.745e-12687.89cilia and flagella associated protein 20 [Source:H... [more]
CFAP202.305e-5183.87cilia and flagella associated protein 20 [Source:H... [more]
C3orf672.854e-1229.24chromosome 3 open reading frame 67 [Source:HGNC Sy... [more]
C3orf672.909e-1229.24chromosome 3 open reading frame 67 [Source:HGNC Sy... [more]
C3orf671.025e-929.24chromosome 3 open reading frame 67 [Source:HGNC Sy... [more]
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BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
C54C6.66.487e-11775.51Cilia- and flagella-associated protein 20 [Source... [more]
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BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Bug221.592e-12586.70gene:FBgn0032248 transcript:FBtr0080044[more]
CG171184.424e-8965.57gene:FBgn0032291 transcript:FBtr0080111[more]
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BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
cfap201.705e-12787.89cilia and flagella associated protein 20 [Source:N... [more]
zgc:1623242.923e-1124.39zgc:162324 [Source:ZFIN;Acc:ZDB-GENE-070410-73][more]
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BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
tjp25.286e-927.49tight junction protein 2 [Source:Xenbase;Acc:XB-GE... [more]
tjp21.163e-827.49tight junction protein 2 [Source:Xenbase;Acc:XB-GE... [more]
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BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Cfap201.152e-12788.42cilia and flagella associated protein 20 [Source:M... [more]
Cfap203.335e-9985.90cilia and flagella associated protein 20 [Source:M... [more]
Cfap201.211e-5883.50cilia and flagella associated protein 20 [Source:M... [more]
Cfap201.094e-1070.59cilia and flagella associated protein 20 [Source:M... [more]
Cfap202.093e-978.13cilia and flagella associated protein 20 [Source:M... [more]
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BLAST of Cilia- and flagella-associated protein 20 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q8BTU1|CFA20_MOUSE8.061e-12788.42Cilia- and flagella-associated protein 20 OS=Mus m... [more]
sp|Q6B857|CFA20_BOVIN8.061e-12788.42Cilia- and flagella-associated protein 20 OS=Bos t... [more]
sp|Q6GL74|CFA20_XENTR9.499e-12787.89Cilia- and flagella-associated protein 20 OS=Xenop... [more]
sp|Q6GPY6|CFA20_XENLA9.499e-12787.89Cilia- and flagella-associated protein 20 OS=Xenop... [more]
sp|Q5ZHP3|CFA20_CHICK9.499e-12787.89Cilia- and flagella-associated protein 20 OS=Gallu... [more]
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BLAST of Cilia- and flagella-associated protein 20 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2T7NIS13.206e-12686.29DUF667 domain-containing protein OS=Pomacea canali... [more]
A0A1I8HHM73.707e-12586.29DUF667 domain-containing protein OS=Macrostomum li... [more]
V4A7R84.081e-12589.47DUF667 domain-containing protein OS=Lottia gigante... [more]
A0A2B4SY994.890e-12587.89UPF0468 protein C16orf80-like OS=Stylophora pistil... [more]
K1R2T76.289e-12588.42UPF0468 protein C16orf80-like protein OS=Crassostr... [more]
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BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
cfap203.392e-12780.37cilia and flagella associated protein 20 [Source:N... [more]
zgc:1623241.707e-723.39chromosome 13 C3orf67 homolog [Source:NCBI gene;Ac... [more]
zgc:1623242.038e-723.39chromosome 13 C3orf67 homolog [Source:NCBI gene;Ac... [more]
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BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000000785.14.968e-12887.37pep scaffold:Pmarinus_7.0:GL477446:372075:392529:1... [more]
ENSPMAT00000002633.11.103e-10988.41pep scaffold:Pmarinus_7.0:GL481839:16843:20127:-1 ... [more]
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BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO359651.631e-12888.95Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO487053.573e-624.84Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Cilia- and flagella-associated protein 20 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cfap203.460e-12183.16cilia and flagella associated protein 20 [Source:N... [more]
ENSORLT00000009678.24.346e-11278.72cilia- and flagella-associated protein 20 [Source:... [more]
ENSORLT00000033906.13.620e-3739.79pep primary_assembly:ASM223467v1:1:15541467:155441... [more]
ENSORLT00000041219.13.969e-3639.00pep primary_assembly:ASM223467v1:1:15541467:155441... [more]
ENSORLT00000038818.17.970e-2651.40pep primary_assembly:ASM223467v1:1:28238078:282410... [more]
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BLAST of Cilia- and flagella-associated protein 20 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000057288.15.851e-146100.00SMESG000057288.1[more]
SMESG000081781.15.653e-13290.58SMESG000081781.1[more]
SMESG000059817.11.050e-11675.52SMESG000059817.1[more]
SMESG000026833.15.503e-6246.03SMESG000026833.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015423 ID=SMED30015423|Name=Cilia- and flagella-associated protein 20|organism=Schmidtea mediterranea sexual|type=transcript|length=1035bp
CTTTGTTTAAGCTAATCACTAAGCTGGCTAATATGACAACGACCTGTTCT
CTATGACATTCTAATTTGTTCAATTTTTTTAAGTTAAGTGAGCAATATTA
TAACGTAATGTTCAGAAACACATATCAGAGTGGGTTTTTATCTATATTAT
ACAGTATAGGTAGTAAGCCATTGCAAATTTGGGGTCGTGAAGTCAAAAAT
GGGCATATTAAAAGAATTACGGATAACGACATTCAATCTTTGGTCTTAGA
AATAATGGGTACAAATGTTAGTACAACTTTCATCAATTGTCCATCTGAAA
ATGAAAAGTCATTAGGTATTAAATTGCCATTTCTTGTAATGATAATCAAA
AATCTGAAAAAATATTTTACATTCGAAGTTCAAATTCTTGACGACAAAAA
CATTAAGCGCCGATTTCGAGCAAGTAATTACCAAAGTACAACACGCGTTC
AGCCTTTCGTATGCACAATGCCAATGCGATTAGATGAAGGCTGGAATCAG
ATTCAGTTCAACTTGTCTGATTTCACTAGAAGAGCATACGGCACAAATTA
CATTGAAACGGTTCGAGTTCAGATTCATGCTAATTGTCGTATTCGTCGAG
TTTACTTTTCTGATCGGCTCTATTCGGAAGATGAGTTACCAGCAGAATTC
AAGCTGTACCTACCGGTTCAAAATAGAAACAATCCTGATGGTCCGCAGTA
GTGCTGGTTGTATACAGTCCCTATTTGTTCAAATTTGAAATATTTCTGTC
ATAAGTATGTGTTGGAGAGAATGTTATTGTGGAAAACGTTGATGTCAACA
AATTATTTATTTGTAATTGATAATATTAAATAAAATTAGAATTATACTTT
CATTTCGTCTTACCAGTTGTCAGGTCTGATCCTGCAACCAACAGAATCTT
CAGTTCATGTTTATATCTGCTAGGAAAAATTGAACAGAATAAGAATAATC
GGAGTCGTAAACATTTTGTTCTTCAGATGTATAAATTGCTTTAGAATGGA
CTTGTATTATCAATGAGTATACAATGTGCACAGAT
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protein sequence of SMED30015423-orf-1

>SMED30015423-orf-1 ID=SMED30015423-orf-1|Name=SMED30015423-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=198bp
MFRNTYQSGFLSILYSIGSKPLQIWGREVKNGHIKRITDNDIQSLVLEIM
GTNVSTTFINCPSENEKSLGIKLPFLVMIIKNLKKYFTFEVQILDDKNIK
RRFRASNYQSTTRVQPFVCTMPMRLDEGWNQIQFNLSDFTRRAYGTNYIE
TVRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNRNNPDGPQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR040441CFA20/C3orf67
IPR007714CFA20_dom
Vocabulary: molecular function
TermDefinition
GO:0003723RNA binding
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005737cytoplasm
GO:0005814centriole
GO:0005856cytoskeleton
GO:0005874microtubule
GO:0005929cilium
GO:0036064ciliary basal body
GO:0042995cell projection
GO:0070062extracellular exosome
Vocabulary: biological process
TermDefinition
GO:0018095protein polyglutamylation
GO:0060271cilium morphogenesis
GO:0060296regulation of cilium beat frequency involved in ciliary motility
GO:2000147positive regulation of cell motility
GO:2000253positive regulation of feeding behavior
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007714CFA20 domainPFAMPF05018DUF667coord: 1..184
e-value: 5.6E-92
score: 306.5
NoneNo IPR availablePANTHERPTHR12458:SF8CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 20coord: 1..189
IPR040441Cilia- and flagella-associated protein 20/C3orf67PANTHERPTHR12458ORF PROTEINcoord: 1..189