SMED30015396

Overview
NameSMED30015396
Smed IDSMED30015396
Length (bp)1665
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30015396

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
reproductive organSMED30015396SMESG000017331.1 Contig27343newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30015396SMESG000017331.1 Contig27343uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
testis primordiumSMED30015396SMESG000017331.1 PL06002X1E08_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism juvenile cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Alignments
SMED30015396 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002835:45910..47574 -8325
Homology
[Close]
BLAST of SMED30015396 vs. TrEMBL
Match: A0A504Z3X1 (Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_11590 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 9.371e-15
Identity = 62/221 (28.05%), Postives = 104/221 (47.06%), Query Frame = 2 
Query:  863 TIEKVFYVIEDLCARLIDQADVPDATSRRNILVVLKSAKENLILLKELSLRVDVWLEGILLGLYSKYRTVVEDXXXXXXXXXXQPILAQTLINNSQFDVAGDIVTALFKAMEFKPYWFWIYNYALGYIIGCSQQFSILEMIIERVMQSKQPIILFYWPNFVYYATRFWESKNLSYKPSIIVKIIQFLEHCITKRLSYGPAKRALIHFSSRSSVICKLFTVW 1525
            T+ +V  V+ +   +L D   V  +T+R  +   + +  ENL L+  L    + WL+ +L  L S  RTV E+ C+++ F      +   + N    D+A D+ TA+  A+E     F  Y   L  +I     FSIL+ +IE    S   ++ + WP  +YYA ++W  K       ++  + + +E    + +S   AK A I  S R  ++ K  TVW
Sbjct:  488 TVNEVEVVLRNFGKQLSDTFQVATSTTREELHSAIAACAENLGLMPVLLRTAEDWLDNLLGCLCSADRTVREETCYVLAFTFTNRTVVDFIRNYPTVDIAYDVTTAVLHAIELYQASFLHYYCLLALMIEGCPMFSILKAVIEIGPHSTSTLLNYIWPRLIYYALKYWNGKQFLRDRVLLKNLNECIEKAYLRGISRRNAKLAQIQLSRRFKILTKPTTVW 708
[Close]
BLAST of SMED30015396 vs. TrEMBL
Match: A0A4E0QZC2 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_008656 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 9.706e-15
Identity = 62/227 (27.31%), Postives = 106/227 (46.70%), Query Frame = 2 
Query:  845 KLIEMRTIEKVFYVIEDLCARLIDQADVPDATSRRNILVVLKSAKENLILLKELSLRVDVWLEGILLGLYSKYRTVVEDXXXXXXXXXXQPILAQTLINNSQFDVAGDIVTALFKAMEFKPYWFWIYNYALGYIIGCSQQFSILEMIIERVMQSKQPIILFYWPNFVYYATRFWESKNLSYKPSIIVKIIQFLEHCITKRLSYGPAKRALIHFSSRSSVICKLFTVW 1525
            +L    T+ +V  V+ +   +L D   V  +T+R  +   + +  ENL L+  L    + WL+ +L  L S  RTV E+ C+++ F      +   + N    D+A D+ TA+  A+E     F  Y   L  +I     FSIL+ ++E    S   ++ + WP  +YYA ++W  K       ++  + + +E    + +S   AK A I  S R  ++ K  TVW
Sbjct:  482 QLFRYLTVNEVEVVLRNFGKQLSDTFQVATSTAREELHSAIAACAENLGLMPVLLRTAEDWLDNLLGCLCSADRTVREETCYVLAFTFTNRTVVDFIRNYPTVDIAYDVTTAVLHAIELHQASFLHYYCLLALMIEGCPMFSILKAVMEIGPHSTSTLLNYIWPRLIYYALKYWNGKQFLRDRVLLKNLNECIEKAYLRGISRRNAKLAQIQLSRRFKILTKPTTVW 708
[Close]
BLAST of SMED30015396 vs. TrEMBL
Match: W6U3Y6 (Uncharacterized protein OS=Echinococcus granulosus OX=6210 GN=EGR_09833 PE=4 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.912e-14
Identity = 66/240 (27.50%), Postives = 114/240 (47.50%), Query Frame = 2 
Query:  827 AVRYDEKLIEMRTIEKVFYVIEDLCARLIDQADVPDATSRRNILVVLKSAKENLILLKELSLRVDVWLEGILLGLYSKYRTVVEDXXXXXXXXXXQPILAQTLINNSQFDVAGDIVTALFKAMEFKPYWFWIYNYALGYII-GCSQQFSILEMIIERVMQSKQPIILFYWPNFVYYATRFWESKNLSYKPSIIVKIIQFLEHCITKRLSYGPAKRALI----HFSSRSSVICKLFTVWPR 1531
            + + DE L++++T +++  +   +   L         T+R  +L+VL ++  N  L   L  +V  WL+ ++ GL+S  + V E+ C+ + ++     LA+ + +   +D+  D+   + KAM+  P  F  Y   LGY++ GCS  FSIL  I+ER      P+I   WP FVYYA + W +  L   P     +++ +       L+   AK AL+     F      +     VW R
Sbjct:  444 SAKADETLLQLKTCKEIKKLYLLMGEELTRSNLGLGGTTRMELLIVLATSINNQRLRHCLWGQVRCWLDPLIGGLFSTSKCVREESCYTLAYMFSINSLAEDIRHLLSYDIPYDVTWNVLKAMDLFPETFMTYFLLLGYMLEGCSS-FSILNCIMERAALCSNPLIQKRWPLFVYYAIKHWNAMYLRVDPVNPALLMEKMTLAAMNPLTRKAAKMALVALAKKFDKSVGAVTHFDPVWTR 682
[Close]
BLAST of SMED30015396 vs. TrEMBL
Match: A0A0X3Q3K7 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=TR155779 PE=4 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 4.398e-14
Identity = 100/390 (25.64%), Postives = 157/390 (40.26%), Query Frame = 2 
Query:  413 KPNIGVILEGYVSHIILKKKLPKDPEH-------RKYMTDLYKMCIDSSSVLKRLVK-KDNEFLPQHLR-----DRIKNYTLSKLSTKTHDDKNKDMDFLYIFPPNPQPVSYEIDGPKCTYASLNDVSAELLKDNLEFKRFYQS-----ILLTRTFA-VRYDEKLIEMRTIEKVFYVIEDLCARLIDQADVPDATSRRNILVVLKSAKENLILLKELSLRVDVWLEGILLGLYSKYRTVVEDXXXXXXXXXXQPILAQTLINNSQFDVAGDIVTALFKAMEFKPYWFWIYNYALGYIIGCSQQFSILEMIIERVMQSKQPIILFYWPNFVYYATRFWESKNLSYKPSIIVKIIQFLEHCITKRLSYGPAKRALIHFSSRSSVICKLFTVW 1525
            KP +  +   Y   +IL  K+     H        + M +LY + I   + LKR+   K+   LP+ L+      R     LS++  K  D  N D  F               D   C  +S+                FY++      L T  F+  RYD +L++++  E+   + + L   L  +   P A +R  +L VL +A     L  ++   V  WLE +L  L ++  +  ++CC+ + ++     L + + +N   D+  D+  A+ +A+E  P  F  Y   LGY+I      SIL  I E+   SK  I    WP  +YYA RFW+   L   P         LE      +S   AK AL   S R+       T W
Sbjct:  340 KPEVEDVRHMYSGMVILSAKIKDFDLHVPLISLDTEEMRELYTVTILRDNSLKRIFNFKNIARLPEELQYLRVLKRSNEPDLSQVKMKDADADN-DAQFRVAM----------ADTSTCILSSIAS-------------HFYEADFLVLGLFTSWFSRARYDPRLMKLQATEEFTKLFQLLGEELFYRGSDPSACTRLELLYVLATASTETKLRHQMWTNVRFWLEPLLSNLIAERSSSRQECCYTLAYLFSSNKLVEDMRHNLTLDIPFDVTAAVVRAIETCPSSFMHYFCLLGYMIEGCPNISILRCITEKGPTSKNLIFRQRWPLLIYYAFRFWKMDYLQVDPLYPKHTAMELEAARKDPISRRAAKMALCAISIRTRSPVPSVTCW 705
[Close]
BLAST of SMED30015396 vs. TrEMBL
Match: A0A183SVI9 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS8237 PE=4 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 4.433e-14
Identity = 69/240 (28.75%), Postives = 108/240 (45.00%), Query Frame = 2 
Query:  809 LLTRTFA-VRYDEKLIEMRTIEKVFYVIEDLCARLIDQADVPDATSRRNILVVLKSAKENLILLKELSLRVDVWLEGILLGLYSKYRTVVEDXXXXXXXXXXQPILAQTLINNSQFDVAGDIVTALFKAMEFKPYWFWIYNYALGYIIGCSQQFSILEMIIERVMQSKQPIILFYWPNFVYYATRFWESKNLSYKPSIIVKIIQFLEHCITKRLSYGPAKRALIHFSSRSSVICKLFTVW 1525
            L T  F+  RYD +L++++  E+   + + L   L  +   P A +R  +L VL +A     L  ++   V  WLE +L  L ++  +  ++CC+ + ++     L + + +N   D+  D+  A+ +A+E  P  F  Y   LGY+I      SIL  I E+   SK  I    WP  +YYA RFW+   L   P         LE      +S   AK AL   S R+       T W
Sbjct:  458 LFTSWFSRARYDPRLMKLQATEEFTKLFQLLGEELFYRGSDPSACTRLELLYVLATASTETKLRHQMWTNVRFWLEPLLSNLIAERSSSRQECCYTLAYLFSSNKLVEDMRHNLTLDIPFDVTAAVVRAIETCPSSFMHYFCLLGYMIEGCPNISILRCITEKGPTSKNLIFRQRWPLLIYYAFRFWKMDYLQVDPLYPKHTAMELEAARKDPISRRAAKMALCAISIRTRSPVPSVTCW 697
[Close]
BLAST of SMED30015396 vs. Planmine SMEST
Match: SMESG000017331.1 (SMESG000017331.1)

HSP 1 Score: 1023.08 bits (2644), Expect = 0.000e+0
Identity = 510/510 (100.00%), Postives = 510/510 (100.00%), Query Frame = 2 
Query:    2 TTIDKFKLVWSGVYFKNITTVEVEEKIVCRSPTRLLRKATASKLGKNIIHTQIPYRPNPIFSMKAGKINLARSSGYSGIDESGTNASLASYGMLEYENYISSRKSSGMNPPSITVPKVVSTPTSKSLHRTVQEELDEKPNIGVILEGYVSHIILKKKLPKDPEHRKYMTDLYKMCIDSSSVLKRLVKKDNEFLPQHLRDRIKNYTLSKLSTKTHDDKNKDMDFLYIFPPNPQPVSYEIDGPKCTYASLNDVSAELLKDNLEFKRFYQSILLTRTFAVRYDEKLIEMRTIEKVFYVIEDLCARLIDQADVPDATSRRNILVVLKSAKENLILLKELSLRVDVWLEGILLGLYSKYRTVVEDXXXXXXXXXXQPILAQTLINNSQFDVAGDIVTALFKAMEFKPYWFWIYNYALGYIIGCSQQFSILEMIIERVMQSKQPIILFYWPNFVYYATRFWESKNLSYKPSIIVKIIQFLEHCITKRLSYGPAKRALIHFSSRSSVICKLFTVWPR 1531
            TTIDKFKLVWSGVYFKNITTVEVEEKIVCRSPTRLLRKATASKLGKNIIHTQIPYRPNPIFSMKAGKINLARSSGYSGIDESGTNASLASYGMLEYENYISSRKSSGMNPPSITVPKVVSTPTSKSLHRTVQEELDEKPNIGVILEGYVSHIILKKKLPKDPEHRKYMTDLYKMCIDSSSVLKRLVKKDNEFLPQHLRDRIKNYTLSKLSTKTHDDKNKDMDFLYIFPPNPQPVSYEIDGPKCTYASLNDVSAELLKDNLEFKRFYQSILLTRTFAVRYDEKLIEMRTIEKVFYVIEDLCARLIDQADVPDATSRRNILVVLKSAKENLILLKELSLRVDVWLEGILLGLYSKYRTVVEDCCFIICFICFQPILAQTLINNSQFDVAGDIVTALFKAMEFKPYWFWIYNYALGYIIGCSQQFSILEMIIERVMQSKQPIILFYWPNFVYYATRFWESKNLSYKPSIIVKIIQFLEHCITKRLSYGPAKRALIHFSSRSSVICKLFTVWPR
Sbjct:    2 TTIDKFKLVWSGVYFKNITTVEVEEKIVCRSPTRLLRKATASKLGKNIIHTQIPYRPNPIFSMKAGKINLARSSGYSGIDESGTNASLASYGMLEYENYISSRKSSGMNPPSITVPKVVSTPTSKSLHRTVQEELDEKPNIGVILEGYVSHIILKKKLPKDPEHRKYMTDLYKMCIDSSSVLKRLVKKDNEFLPQHLRDRIKNYTLSKLSTKTHDDKNKDMDFLYIFPPNPQPVSYEIDGPKCTYASLNDVSAELLKDNLEFKRFYQSILLTRTFAVRYDEKLIEMRTIEKVFYVIEDLCARLIDQADVPDATSRRNILVVLKSAKENLILLKELSLRVDVWLEGILLGLYSKYRTVVEDCCFIICFICFQPILAQTLINNSQFDVAGDIVTALFKAMEFKPYWFWIYNYALGYIIGCSQQFSILEMIIERVMQSKQPIILFYWPNFVYYATRFWESKNLSYKPSIIVKIIQFLEHCITKRLSYGPAKRALIHFSSRSSVICKLFTVWPR 511
[Close]
BLAST of SMED30015396 vs. Planmine SMEST
Match: SMESG000050073.1 (SMESG000050073.1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.069e-21
Identity = 81/346 (23.41%), Postives = 163/346 (47.11%), Query Frame = 2 
Query:  497 KYMTDLYKMCIDSSSVLKRLVKKDNEFLPQHLRDRIKNYTLSKLSTKTHDDKNKDMDFLYIFPPNPQPVSYEIDGPKCTYASLNDVSAELLKDNLEFKRFYQSILLTRTFAVRYDEKLIEMRTIEKVFYVIEDLCARLIDQADVPDATSRRNILVVLKSAKENLILLKELSLRVDVWLEGILLGLYSKYRTVVEDXXXXXXXXXXQPILAQTLINNSQFDVAGDIVTALFKAMEFKPYWFWIYNYALGYIIGCSQQFSILEMIIERVMQSKQPIILFYWPNFVYYATRFWESKNLSYKPSIIVKIIQFLE-HCITKRLSYGPAKRALIHFSSRSSVICKLFTVWPR 1531
            K   ++ K C+ ++ V+ +L+ K N F P  L+ ++     S LS    D  +K +D      P P+ +S +       +  + D+ +E+  D  EF  FY+ ++      V+  + L +    EK+   I+ L   L    + P +T+R      +K+ + N ++L EL    D WL+ IL    SK+ ++  +    +  +  +P+    + ++   D+  D++  + +  + K   + ++   +G I   +    +++ +IER   S    I+  WP F+Y+  ++WES N +       ++I+ LE H + + LS    K+A++   +R +V   +FT WP+
Sbjct:  319 KIENNIMKHCVLNTKVINKLMNKKN-FYPYRLQLKMAEIDAS-LSVTHEDAASKAID-----QPMPKILSKKTKTNSKMHFKIQDILSEVCGDIKEFNGFYRPLIEYCIKNVKDFKDLEKETNAEKIKKYIQSLGYTLTIHRNHPGSTARTLTQQFIKAGEVNSLILNELINNADGWLDQILKSFLSKFPSIRLEAFVTVAVLFSKPLYVTAIQSHLFSDIIYDVLATILRCFDLKSEDYEMHYSLIGVIFHNAPCQELIDCLIERAPYSYHVEIVSKWPRFLYFGMKYWESTNFNTSSIHFKRMIETLESHYLRESLSRKYLKQAILCICARFNVPSTMFTSWPK 657
[Close]
BLAST of SMED30015396 vs. Planmine SMEST
Match: SMESG000032114.1 (SMESG000032114.1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.069e-21
Identity = 81/346 (23.41%), Postives = 163/346 (47.11%), Query Frame = 2 
Query:  497 KYMTDLYKMCIDSSSVLKRLVKKDNEFLPQHLRDRIKNYTLSKLSTKTHDDKNKDMDFLYIFPPNPQPVSYEIDGPKCTYASLNDVSAELLKDNLEFKRFYQSILLTRTFAVRYDEKLIEMRTIEKVFYVIEDLCARLIDQADVPDATSRRNILVVLKSAKENLILLKELSLRVDVWLEGILLGLYSKYRTVVEDXXXXXXXXXXQPILAQTLINNSQFDVAGDIVTALFKAMEFKPYWFWIYNYALGYIIGCSQQFSILEMIIERVMQSKQPIILFYWPNFVYYATRFWESKNLSYKPSIIVKIIQFLE-HCITKRLSYGPAKRALIHFSSRSSVICKLFTVWPR 1531
            K   ++ K C+ ++ V+ +L+ K N F P  L+ ++     S LS    D  +K +D      P P+ +S +       +  + D+ +E+  D  EF  FY+ ++      V+  + L +    EK+   I+ L   L    + P +T+R      +K+ + N ++L EL    D WL+ IL    SK+ ++  +    +  +  +P+    + ++   D+  D++  + +  + K   + ++   +G I   +    +++ +IER   S    I+  WP F+Y+  ++WES N +       ++I+ LE H + + LS    K+A++   +R +V   +FT WP+
Sbjct:  319 KIENNIMKHCVLNTKVINKLMNKKN-FYPYRLQLKMAEIDAS-LSVTHEDAASKAID-----QPMPKILSKKTKTNSKMHFKIQDILSEVCGDIKEFNGFYRPLIEYCIKNVKDFKDLEKETNAEKIKKYIQSLGYTLTIHRNHPGSTARTLTQQFIKAGEVNSLILNELINNADGWLDQILKSFLSKFPSIRLEAFVTVAVLFSKPLYVTAIQSHLFSDIIYDVLATILRCFDLKSEDYEMHYSLIGVIFHNAPCQELIDCLIERAPYSYHVEIVSKWPRFLYFGMKYWESTNFNTSSIHFKRMIETLESHYLRESLSRKYLKQAILCICARFNVPSTMFTSWPK 657
[Close]
BLAST of SMED30015396 vs. Planmine SMEST
Match: SMESG000025877.1 (SMESG000025877.1)

HSP 1 Score: 96.6709 bits (239), Expect = 6.821e-21
Identity = 99/405 (24.44%), Postives = 175/405 (43.21%), Query Frame = 2 
Query:  347 PKVVSTPTSKSLHRTVQ-------EELDEKPNIGVILEGYVSHIILKKKLPKDPEHRKYMTDLYKMCIDSSSVLKRLVKKDNEFLPQHLRDRIKNYTLSKLSTKTHDDKNKDMDFLYIFPPNPQPVSYEI-DGPKCTYASLN----DVSAELLKDNLEFKRFYQSILLTRTFAVRYDEKLIEMRTIEKVFYVIEDLCARLIDQADVPDATSRRNILVVLKSAKENLILLKELSLRVDVWLEGILLGLYSKYRTVVEDXXXXXXXXXXQPILAQTLINNSQFDVAGDIVTALFKAMEFKPYWFWIYNYALGYIIGCSQQFSILEMIIERVMQSKQPIILFYWPNFVYYATRFWESKNLSYKPSIIVKIIQFLEHCITKRLSYGPAKRALIHFSSRSSVICKLFTVW 1525
            PK VST  S    RT         EE++EKP  G+++  YV   ILK  LP    + + + +LY  CI     L RL     + L + L D I ++  S  +                    P+ +  EI D  +  + S+N    D+  E+  D+LEF  F +   +     V  D  L+ ++ + ++  +I+ + + L      P + +R+ ++     A  N  +    S  +  WLE ++ G +S +  + ED C+ + F+  Q      L+N S  +   DI+  +FKA++     F  +   +GYI+      SIL  I ++   SK  ++   WP FV +A + W +++      ++ K+   LE      +++  +K AL +   R +      T W
Sbjct:  175 PKPVSTSKSLGSSRTGDNFRLPYIEEINEKPKAGILVSCYVKDHILK--LPILMANSERIRELYTNCILKDEHLNRLFTTKKDMLSKDLIDYIADFGTSNETENA-----------------PKVIKDEIADRDQFFHTSMNVHANDILREIEIDSLEFSVFIKPCYILNFSQVMPDISLMSLQDVSQIEEIIKSILSGLESDILNPVSPARKKLMSAFLIANSNANVASVFSDMLPTWLEVLVKGFFSPHSPIREDTCYCVGFLFTQSKFLLELLNYSS-EFIYDIIAIIFKAIDHYSSSFEFFYSLIGYIMDGCPVVSILLCICDKGPFSKNYLMRKQWPKFVVFACKHWPNESFKSGEVLVKKVQDLLEKFFLHPITHKTSKMALAYIGRRFNRKAMTITHW 559
[Close]
BLAST of SMED30015396 vs. Planmine SMEST
Match: SMESG000065924.1 (SMESG000065924.1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.664e-20
Identity = 114/458 (24.89%), Postives = 194/458 (42.36%), Query Frame = 2 
Query:  167 RPNPIFSMKAGKINLARSSGYSGIDESGTNASLASYGMLEYENYISSRKSSGMNPPSITVPKVVSTPTSKSLHRTVQEELDEKPNIGVILEGYVSHIILKKKLPK-DPEHRKYMTDLYKMCIDSSSVLKRLVKKDNEFLPQHLRDRI----KNYTLSKLSTKTHDDKNKDMDFLYIFPPNPQPVSYEIDGPKCTYASLNDVSAELLKDNLEFKRFYQSILLTRTFAVRYDEKLIEMRTIEKVFYVIEDLCARLIDQADVPDATSRRNILVVLKSAKENLILLKELSLRVDVWLEGILLGLYSKYRTVVEDXXXXXXXXXXQPILAQTLINNSQFDVAGDIVTALFKAMEFKPYWFWIYNYALGYIIGCSQQFSILEMIIERVMQSKQPIILFYWPNFVYYATRFWESKNLSYKPSIIVKIIQFLEHCITKRLSYGPAKRALIHFSSRSSVICKLFTVW 1525
            R N + SM + +   +    Y  I     ++SL S   L  E+Y       G N   +T      T  S  LH+ + +  D  P    +L  YVS   LKK     + EH     +LY   I  +  L  +++K  +  P+ L   I    KN + S+ +TK  D+  KD+ F      N             +Y S  D   E+ +D  +   FY  +       VR    L E+   E     I  L   L +  + PD+  R  ++  +KS + N  L   ++     WL  ++  L+S++ ++ E+ C ++ +I  +  +   L+N+ Q+DV+ DI  ++ + +E  P         +GYI+  S    ILE +IE+    K P +   WP  +YY  ++W    +      + KI + LE  + K ++   A+  L   S +  ++ K+ TVW
Sbjct:  130 RSNSVLSMSSARSFDSSELKYHHISLVNRDSSLVS--TLSSEDY-------GKNDQVMTR----GTFLSNHLHKMIPDMKDAVP-AEELLNMYVSEYHLKKPFTSAEKEH----IELYNNVIKKNKSLFDMLQKHTDIFPKDLVHLIQHFKKNISDSEENTKIVDNFEKDLYF------NSSLTK--------SYPSFID---EITEDLTDLTVFYYPLFKATFSLVRSVNYLTEIEDEEYFRREIYRLNLELKNNRNRPDSLVRWKVMEFVKSCEHNYTLTLTMAQTCGSWLNNLIKCLFSEFSSIREETCIVLAYIMSRQTITNELLNSRQYDVSYDIFVSILRVIEQYPENISSLYCLIGYILHRSVSVEILEYLIEKGPMLKNPFMRKRWPKIIYYGLKYWPRGRMLDNAGTLRKIKESLELMVLKTVTKKYARMTLYFLSRKYLLMSKMGTVW 552
The following BLAST results are available for this feature:
BLAST of SMED30015396 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015396 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015396 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015396 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015396 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015396 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015396 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015396 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A504Z3X19.371e-1528.05Uncharacterized protein OS=Fasciola gigantica OX=4... [more]
A0A4E0QZC29.706e-1527.31Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
W6U3Y61.912e-1427.50Uncharacterized protein OS=Echinococcus granulosus... [more]
A0A0X3Q3K74.398e-1425.64Uncharacterized protein OS=Schistocephalus solidus... [more]
A0A183SVI94.433e-1428.75Uncharacterized protein OS=Schistocephalus solidus... [more]
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BLAST of SMED30015396 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015396 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015396 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015396 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015396 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015396 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000017331.10.000e+0100.00SMESG000017331.1[more]
SMESG000050073.11.069e-2123.41SMESG000050073.1[more]
SMESG000032114.11.069e-2123.41SMESG000032114.1[more]
SMESG000025877.16.821e-2124.44SMESG000025877.1[more]
SMESG000065924.11.664e-2024.89SMESG000065924.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015396 ID=SMED30015396|Name=SMED30015396|organism=Schmidtea mediterranea sexual|type=transcript|length=1665bp
GACGACAATTGATAAATTTAAACTTGTATGGTCAGGAGTTTATTTTAAGA
ATATAACTACAGTTGAAGTGGAAGAGAAAATAGTTTGCAGGTCTCCTACA
AGATTATTAAGAAAAGCTACAGCGAGTAAACTTGGTAAGAACATCATCCA
TACTCAAATACCATACAGACCTAATCCAATTTTTTCCATGAAAGCTGGGA
AGATCAATTTGGCAAGATCTAGTGGATATTCAGGTATAGATGAATCGGGC
ACTAATGCTTCTCTGGCTTCTTATGGAATGTTGGAATACGAGAACTATAT
TTCCTCACGAAAATCATCTGGAATGAATCCTCCTTCTATTACTGTTCCTA
AAGTCGTTTCAACACCCACAAGTAAATCTTTGCATCGCACAGTTCAAGAG
GAATTAGATGAAAAGCCAAACATTGGAGTAATTCTGGAAGGCTATGTTAG
TCACATTATTTTAAAAAAGAAACTTCCTAAAGATCCTGAACATAGGAAAT
ACATGACTGATCTTTACAAAATGTGCATAGATAGTTCATCAGTTTTGAAA
CGACTTGTAAAAAAGGATAACGAATTTTTGCCCCAACACTTGAGAGACAG
AATTAAAAACTATACTTTATCTAAGCTTTCCACAAAAACTCACGATGACA
AAAATAAAGACATGGATTTCCTTTATATATTTCCACCAAACCCGCAGCCG
GTAAGCTATGAAATTGATGGTCCAAAATGTACATATGCATCCTTAAATGA
TGTGAGTGCTGAATTGTTAAAAGATAATCTAGAATTCAAGCGTTTCTATC
AAAGTATATTACTAACAAGAACCTTTGCAGTTAGATATGACGAAAAACTC
ATCGAAATGAGAACCATAGAGAAAGTTTTTTATGTCATAGAAGATTTGTG
TGCTAGATTAATAGATCAAGCCGATGTCCCAGATGCAACATCAAGAAGAA
ATATCTTAGTGGTTTTAAAAAGCGCCAAAGAAAATCTAATACTTTTAAAA
GAATTGAGTCTCAGAGTTGATGTATGGTTGGAAGGTATTTTGTTGGGATT
GTACAGTAAATACCGAACAGTTGTGGAAGACTGTTGCTTTATTATTTGTT
TCATTTGTTTCCAGCCGATTCTTGCTCAGACTTTGATAAATAATTCTCAA
TTCGATGTAGCAGGAGACATTGTAACGGCGCTTTTTAAAGCAATGGAATT
TAAGCCATATTGGTTTTGGATATACAATTATGCGTTAGGCTATATTATTG
GATGCTCTCAACAGTTTTCAATTCTCGAAATGATTATTGAAAGGGTTATG
CAATCCAAACAGCCAATCATTTTATTTTATTGGCCAAACTTTGTTTATTA
TGCGACTAGATTTTGGGAGTCTAAGAACCTCTCCTACAAACCAAGCATTA
TTGTAAAGATTATTCAATTTTTGGAGCATTGTATCACTAAACGACTGTCC
TACGGCCCGGCGAAACGAGCACTTATACATTTCAGCTCTAGGAGTTCCGT
TATTTGTAAATTATTTACAGTATGGCCCCGCTAATGTCTGTGTGTAAATT
CCTATTGTATTTTGAAGATTTTTTTTTCAAATAATTTTCCACTATTTCCT
TATAGTTGAGTTATTTGAATTAAACTTACGTACTTCCATAAAATGTAAGA
AATAATAAAAAAAAC
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protein sequence of SMED30015396-orf-1

>SMED30015396-orf-1 ID=SMED30015396-orf-1|Name=SMED30015396-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=511bp
TTIDKFKLVWSGVYFKNITTVEVEEKIVCRSPTRLLRKATASKLGKNIIH
TQIPYRPNPIFSMKAGKINLARSSGYSGIDESGTNASLASYGMLEYENYI
SSRKSSGMNPPSITVPKVVSTPTSKSLHRTVQEELDEKPNIGVILEGYVS
HIILKKKLPKDPEHRKYMTDLYKMCIDSSSVLKRLVKKDNEFLPQHLRDR
IKNYTLSKLSTKTHDDKNKDMDFLYIFPPNPQPVSYEIDGPKCTYASLND
VSAELLKDNLEFKRFYQSILLTRTFAVRYDEKLIEMRTIEKVFYVIEDLC
ARLIDQADVPDATSRRNILVVLKSAKENLILLKELSLRVDVWLEGILLGL
YSKYRTVVEDCCFIICFICFQPILAQTLINNSQFDVAGDIVTALFKAMEF
KPYWFWIYNYALGYIIGCSQQFSILEMIIERVMQSKQPIILFYWPNFVYY
ATRFWESKNLSYKPSIIVKIIQFLEHCITKRLSYGPAKRALIHFSSRSSV
ICKLFTVWPR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002089reproductive organ
PLANA:0003304testis primordium
Vocabulary: INTERPRO
TermDefinition
IPR006588Peptide_N_glycanase_PAW_dom
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
Vocabulary: biological process
TermDefinition
GO:0006516glycoprotein catabolic process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 403..425