Transmembrane GTPase Marf

Overview
NameTransmembrane GTPase Marf
Smed IDSMED30015381
Length (bp)1825
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Transmembrane GTPase Marf (SMED30015381) t-SNE clustered cells

Violin plots show distribution of expression levels for Transmembrane GTPase Marf (SMED30015381) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Transmembrane GTPase Marf (SMED30015381) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Transmembrane GTPase Marf (SMED30015381) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30015381

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30015381h1SMcG0004907 Contig261newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30015381h1SMcG0010399 Contig261newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30015381h1SMcG0004907 Contig260newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30015381h1SMcG0004907 Contig249newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30015381h1SMcG0010399 Contig260newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30015381h1SMcG0010399 Contig249newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30015381h1SMcG0010399 dd_Smed_v4_5932_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30015381h1SMcG0010399 dd_Smed_v4_5932_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
oocyteSMED30015381h1SMcG0004907 Contig260newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30015381h1SMcG0004907 Contig260newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
oocyteSMED30015381h1SMcG0010399 Contig260newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30015381h1SMcG0010399 Contig260newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Human
Match: MFN1 (mitofusin 1 [Source:HGNC Symbol;Acc:HGNC:18262])

HSP 1 Score: 49.6766 bits (117), Expect = 1.895e-6
Identity = 20/50 (40.00%), Postives = 35/50 (70.00%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLVVNN 456
             G+TSSGKS++INA L + +LP+  G  +NCF+ ++G+ + ++ YL+   
Sbjct:   81 FGRTSSGKSSVINAMLWDKVLPSGIGHITNCFLSVEGT-DGDKAYLMTEG 129
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Celegans
Match: fzo-1 (Transmembrane GTPase fzo-1 [Source:UniProtKB/Swiss-Prot;Acc:Q23424])

HSP 1 Score: 52.373 bits (124), Expect = 9.080e-7
Identity = 24/55 (43.64%), Postives = 37/55 (67.27%), Query Frame = 1 
Query:  301 VVLGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLVVNNEPR 465
            V  G+TS+GKST INA L E +LP   G T+ CF++++GS E    +L +++ P+
Sbjct:  104 VFFGRTSNGKSTTINAMLHEKVLPQGMGHTTCCFLQVEGS-EGEVGHLQLDDNPQ 157
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Fly
Match: Marf (gene:FBgn0029870 transcript:FBtr0343373)

HSP 1 Score: 51.6026 bits (122), Expect = 2.211e-6
Identity = 20/38 (52.63%), Postives = 30/38 (78.95%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGS 420
             G+TS+GKS++INA L E +LP+  G T+NCF +++GS
Sbjct:  143 FGRTSNGKSSVINAMLREKILPSGIGHTTNCFCQVEGS 180
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Fly
Match: Marf (gene:FBgn0029870 transcript:FBtr0070910)

HSP 1 Score: 51.6026 bits (122), Expect = 2.284e-6
Identity = 20/38 (52.63%), Postives = 30/38 (78.95%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGS 420
             G+TS+GKS++INA L E +LP+  G T+NCF +++GS
Sbjct:  143 FGRTSNGKSSVINAMLREKILPSGIGHTTNCFCQVEGS 180
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Fly
Match: Marf (gene:FBgn0029870 transcript:FBtr0070909)

HSP 1 Score: 51.6026 bits (122), Expect = 2.284e-6
Identity = 20/38 (52.63%), Postives = 30/38 (78.95%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGS 420
             G+TS+GKS++INA L E +LP+  G T+NCF +++GS
Sbjct:  143 FGRTSNGKSSVINAMLREKILPSGIGHTTNCFCQVEGS 180
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Zebrafish
Match: MFN1 (mitofusin 1a [Source:NCBI gene;Acc:100329462])

HSP 1 Score: 53.5286 bits (127), Expect = 5.372e-7
Identity = 28/73 (38.36%), Postives = 44/73 (60.27%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLVV-NNEPRYKQEAKKILCDILRLPDNE 522
             G+TS+GKST+INA L E +LP+  G T+NCF+ ++G+ +  + YL   ++E R   E    L   L +  N+
Sbjct:   82 FGRTSNGKSTIINAMLRERVLPSGIGHTTNCFLSVEGT-DGEQAYLTTEDSEERKGIETVNHLAHALHMNRNQ 153
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Zebrafish
Match: mfn1b (mitofusin 1b [Source:ZFIN;Acc:ZDB-GENE-040426-1553])

HSP 1 Score: 50.447 bits (119), Expect = 3.217e-6
Identity = 20/40 (50.00%), Postives = 31/40 (77.50%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLE 426
             G+TS+GKST+INA L + +LP+  G T+NCF+ ++G+ E
Sbjct:   82 FGRTSNGKSTVINAMLRDRVLPSGIGHTTNCFLSVEGTDE 121
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Zebrafish
Match: mfn2 (mitofusin 2 [Source:ZFIN;Acc:ZDB-GENE-081105-44])

HSP 1 Score: 51.2174 bits (121), Expect = 3.584e-6
Identity = 21/52 (40.38%), Postives = 37/52 (71.15%), Query Frame = 1 
Query:  301 VVLGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLVVNN 456
            V  G+TS+GKS++INA L + +LP+  G T+NCF+ ++G+ + N ++L+   
Sbjct:  100 VFFGRTSNGKSSVINAMLWDKVLPSGIGHTTNCFLRVEGT-DGNESFLLTEG 150
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Zebrafish
Match: mfn1b (mitofusin 1b [Source:ZFIN;Acc:ZDB-GENE-040426-1553])

HSP 1 Score: 50.447 bits (119), Expect = 3.644e-6
Identity = 20/40 (50.00%), Postives = 31/40 (77.50%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLE 426
             G+TS+GKST+INA L + +LP+  G T+NCF+ ++G+ E
Sbjct:   82 FGRTSNGKSTVINAMLRDRVLPSGIGHTTNCFLSVEGTDE 121
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Zebrafish
Match: mfn1b (mitofusin 1b [Source:ZFIN;Acc:ZDB-GENE-040426-1553])

HSP 1 Score: 51.2174 bits (121), Expect = 3.744e-6
Identity = 19/37 (51.35%), Postives = 30/37 (81.08%), Query Frame = 1 
Query:  310 GKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGS 420
            G+TS+GKST+INA L + +LP+  G T+NCF+ ++G+
Sbjct:   83 GRTSNGKSTVINAMLRDRVLPSGIGHTTNCFLSVEGT 119
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Xenopus
Match: mfn1 (mitofusin 1 [Source:NCBI gene;Acc:548943])

HSP 1 Score: 53.1434 bits (126), Expect = 1.364e-6
Identity = 22/47 (46.81%), Postives = 36/47 (76.60%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLV 447
             G+TSSGKST+IN+ L + +LP+  G T+NCF+ ++G+ E ++ YL+
Sbjct:   92 FGRTSSGKSTVINSMLWDKVLPSGIGHTTNCFLSVEGT-EGDKAYLM 137
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Xenopus
Match: mfn1 (mitofusin 1 [Source:NCBI gene;Acc:548943])

HSP 1 Score: 52.7582 bits (125), Expect = 1.432e-6
Identity = 22/47 (46.81%), Postives = 36/47 (76.60%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLV 447
             G+TSSGKST+IN+ L + +LP+  G T+NCF+ ++G+ E ++ YL+
Sbjct:  100 FGRTSSGKSTVINSMLWDKVLPSGIGHTTNCFLSVEGT-EGDKAYLM 145
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Xenopus
Match: mfn1 (mitofusin 1 [Source:NCBI gene;Acc:548943])

HSP 1 Score: 52.7582 bits (125), Expect = 1.525e-6
Identity = 22/47 (46.81%), Postives = 36/47 (76.60%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLV 447
             G+TSSGKST+IN+ L + +LP+  G T+NCF+ ++G+ E ++ YL+
Sbjct:   92 FGRTSSGKSTVINSMLWDKVLPSGIGHTTNCFLSVEGT-EGDKAYLM 137
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Xenopus
Match: mfn1 (mitofusin 1 [Source:NCBI gene;Acc:548943])

HSP 1 Score: 52.7582 bits (125), Expect = 1.542e-6
Identity = 22/47 (46.81%), Postives = 36/47 (76.60%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLV 447
             G+TSSGKST+IN+ L + +LP+  G T+NCF+ ++G+ E ++ YL+
Sbjct:   97 FGRTSSGKSTVINSMLWDKVLPSGIGHTTNCFLSVEGT-EGDKAYLM 142
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Xenopus
Match: mfn1 (mitofusin 1 [Source:NCBI gene;Acc:548943])

HSP 1 Score: 52.7582 bits (125), Expect = 1.560e-6
Identity = 22/47 (46.81%), Postives = 36/47 (76.60%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLV 447
             G+TSSGKST+IN+ L + +LP+  G T+NCF+ ++G+ E ++ YL+
Sbjct:   82 FGRTSSGKSTVINSMLWDKVLPSGIGHTTNCFLSVEGT-EGDKAYLM 127
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Mouse
Match: Mfn1 (mitofusin 1 [Source:MGI Symbol;Acc:MGI:1914664])

HSP 1 Score: 51.6026 bits (122), Expect = 4.316e-7
Identity = 21/47 (44.68%), Postives = 36/47 (76.60%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLV 447
             G+TSSGKS++INA L + +LP+  G T+NCF+ ++G+ + ++ YL+
Sbjct:   81 FGRTSSGKSSVINAMLWDKVLPSGIGHTTNCFLSVEGT-DGDKAYLM 126
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Mouse
Match: Mfn1 (mitofusin 1 [Source:MGI Symbol;Acc:MGI:1914664])

HSP 1 Score: 51.6026 bits (122), Expect = 8.564e-7
Identity = 21/47 (44.68%), Postives = 36/47 (76.60%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLV 447
             G+TSSGKS++INA L + +LP+  G T+NCF+ ++G+ + ++ YL+
Sbjct:   81 FGRTSSGKSSVINAMLWDKVLPSGIGHTTNCFLSVEGT-DGDKAYLM 126
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Mouse
Match: Mfn1 (mitofusin 1 [Source:MGI Symbol;Acc:MGI:1914664])

HSP 1 Score: 51.6026 bits (122), Expect = 2.992e-6
Identity = 21/47 (44.68%), Postives = 36/47 (76.60%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLV 447
             G+TSSGKS++INA L + +LP+  G T+NCF+ ++G+ + ++ YL+
Sbjct:   81 FGRTSSGKSSVINAMLWDKVLPSGIGHTTNCFLSVEGT-DGDKAYLM 126
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Mouse
Match: Mfn1 (mitofusin 1 [Source:MGI Symbol;Acc:MGI:1914664])

HSP 1 Score: 51.6026 bits (122), Expect = 2.992e-6
Identity = 21/47 (44.68%), Postives = 36/47 (76.60%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLV 447
             G+TSSGKS++INA L + +LP+  G T+NCF+ ++G+ + ++ YL+
Sbjct:   81 FGRTSSGKSSVINAMLWDKVLPSGIGHTTNCFLSVEGT-DGDKAYLM 126
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Mouse
Match: Mfn2 (mitofusin 2 [Source:MGI Symbol;Acc:MGI:2442230])

HSP 1 Score: 48.9062 bits (115), Expect = 5.210e-6
Identity = 19/37 (51.35%), Postives = 29/37 (78.38%), Query Frame = 1 
Query:  310 GKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGS 420
            G+TS+GKST+INA L + +LP+  G T+NCF+ + G+
Sbjct:  103 GRTSNGKSTVINAMLWDKVLPSGIGHTTNCFLRVGGT 139
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Cavefish
Match: ENSAMXT00000043809.1 (mitofusin-1-like [Source:NCBI gene;Acc:103034819])

HSP 1 Score: 53.9138 bits (128), Expect = 4.488e-7
Identity = 24/55 (43.64%), Postives = 37/55 (67.27%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLVVNNEPRYK 471
             G+TS+GKST+INA L E +LP+  G T+NCFI ++G+ + ++ +L   N    K
Sbjct:   82 FGRTSNGKSTVINAMLRERVLPSGIGHTTNCFISVEGT-DGDQAFLTTENSEERK 135
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Cavefish
Match: mfn1b (mitofusin-1 [Source:NCBI gene;Acc:103030239])

HSP 1 Score: 51.6026 bits (122), Expect = 2.311e-6
Identity = 22/46 (47.83%), Postives = 35/46 (76.09%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYL 444
             G+TS+GKST+INA L + +LP+  G T+NCF+ ++G+ E ++ YL
Sbjct:   82 FGRTSNGKSTVINAMLRDRVLPSGIGHTTNCFLSVEGTDE-DKAYL 126
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Cavefish
Match: mfn1b (mitofusin-1 [Source:NCBI gene;Acc:103030239])

HSP 1 Score: 51.6026 bits (122), Expect = 2.401e-6
Identity = 22/46 (47.83%), Postives = 35/46 (76.09%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYL 444
             G+TS+GKST+INA L + +LP+  G T+NCF+ ++G+ E ++ YL
Sbjct:   82 FGRTSNGKSTVINAMLRDRVLPSGIGHTTNCFLSVEGTDE-DKAYL 126
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Cavefish
Match: mfn2 (mitofusin 2 [Source:ZFIN;Acc:ZDB-GENE-081105-44])

HSP 1 Score: 50.8322 bits (120), Expect = 4.274e-6
Identity = 22/57 (38.60%), Postives = 37/57 (64.91%), Query Frame = 1 
Query:  301 VVLGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLVVNNEPRYK 471
            V  G+TS+GKS++INA L + +LP+  G T+NCF+ ++G+ + N  +L+       K
Sbjct:  100 VFFGRTSNGKSSVINAMLWDKVLPSGIGHTTNCFLRVEGT-DGNEAFLLTEGSEERK 155
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Nematostella
Match: EDO42379 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1L1])

HSP 1 Score: 50.447 bits (119), Expect = 1.778e-6
Identity = 21/53 (39.62%), Postives = 35/53 (66.04%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLVVNNEPR 465
             G+TS+GKST++NA L + +LP   G T+NCF+ + GS  ++   L+  ++ R
Sbjct:  101 FGRTSNGKSTVVNAMLQDRILPMGIGHTTNCFLSVNGSDSADPYILIPESDER 153
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Medaka
Match: mfn1b (mitofusin 1 [Source:NCBI gene;Acc:101158009])

HSP 1 Score: 53.5286 bits (127), Expect = 5.504e-7
Identity = 22/46 (47.83%), Postives = 36/46 (78.26%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYL 444
             G+TS+GKST+INA L + +LP+  G T+NCF+ ++G+ + +R+YL
Sbjct:   93 FGRTSNGKSTVINAMLRDRVLPSGIGHTTNCFLSVEGT-DDDRSYL 137
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Medaka
Match: mfn1b (mitofusin 1 [Source:NCBI gene;Acc:101158009])

HSP 1 Score: 53.5286 bits (127), Expect = 6.226e-7
Identity = 22/46 (47.83%), Postives = 36/46 (78.26%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYL 444
             G+TS+GKST+INA L + +LP+  G T+NCF+ ++G+ + +R+YL
Sbjct:   93 FGRTSNGKSTVINAMLRDRVLPSGIGHTTNCFLSVEGT-DDDRSYL 137
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Medaka
Match: ENSORLT00000009003.2 (pep primary_assembly:ASM223467v1:17:11595384:11602171:-1 gene:ENSORLG00000007178.2 transcript:ENSORLT00000009003.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.9878 bits (123), Expect = 8.734e-7
Identity = 34/107 (31.78%), Postives = 58/107 (54.21%), Query Frame = 1 
Query:  307 LGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLVVN-NEPRYKQEAKKILCDILRLPDNEIES----KIFFISANERMAIFTNEVTAISAPKWD 612
             G+TS+GKST+INA L + +LP+  G T+NCF+ ++G+ + +  YL    +E R        L   L + D  ++S    K+F+     R A+  N++  + +P  D
Sbjct:   80 FGRTSNGKSTVINAMLRDRVLPSGIGHTTNCFLSVEGT-DGDEAYLTSEASEERRSISTVNQLAHALHM-DPTLDSSCLVKVFW--PKSRCALLRNDLVLMDSPGTD 182
[Close]
BLAST of Transmembrane GTPase Marf vs. Ensembl Medaka
Match: mfn2 (mitofusin 2 [Source:NCBI gene;Acc:101170481])

HSP 1 Score: 51.9878 bits (123), Expect = 1.480e-6
Identity = 20/40 (50.00%), Postives = 31/40 (77.50%), Query Frame = 1 
Query:  301 VVLGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGS 420
            V  G+TS+GKST+INA L + +LP+  G T+NCF+ ++G+
Sbjct:  100 VFFGRTSNGKSTVINAMLCDKVLPSGIGHTTNCFLRVEGT 139
[Close]
BLAST of Transmembrane GTPase Marf vs. Planmine SMEST
Match: SMESG000003246.1 (SMESG000003246.1)

HSP 1 Score: 518.85 bits (1335), Expect = 6.882e-178
Identity = 337/400 (84.25%), Postives = 345/400 (86.25%), Query Frame = 1 
Query:  457 EPRYKQEAKKILCDILRLPDNEIESKIFFISANERMAIFTNEVTAISAPKWDERNNEFASLFSYLQTKMSESADCKKFADLNNTYLETLANFNKCRCLIDEMKDKRIXXXXXXXXXXXXXXDFNSKINSEISAIFNDSYTDLLIKGXXXXXXXXXXXXXXXSNEEKYSLLEHETIQDQTVYGEGLRLSENEFEELLHQIIENSILKLTKRIIELSQSADDMDSFMLNKIFDIYFSQYLNLSEQLSHFDVGSLENNLIEI--------FAVIGLADAVIXXXXXXXXXXXX----------------------XXXXXXXXXXXXXXXXXPFPLAWKKNHLAIRSMWNSFESVLTDSYNRFRDNMTSNXXXXXXXXXAKAMKIRNNLNSIAEDLNNFPNRP 1566
            +PRY QEAKKILCDILRLPDNEIESKIFFISANERMAIFTNEVTAISAPKWDERNNEFASLFSYLQ KMSESADCKKFADLNNTYLE LANFNKCRCLIDEMKDKRIELAAKLKNELKKLEDFNSKINSEISAIFNDSY DLLIKGKELLKKNPQLLNKNNSNEEKY LLE ETIQDQTVYGE LRLSENEFEELLHQIIENSILKLTKRIIELSQSA+DMDSFMLNKIFDIYFSQYLNLSEQLSHFDVGSLENNLIEI           IGL  AV  AG   GGA                         GV+G GAA GAVKA+KLPF  AWK+N LAI++MWNSFESVLTDSY+RFRDNMTSNITEIITTIEAKAMKIRNNLNSIAEDLNNFPNRP
Sbjct:  247 KPRYIQEAKKILCDILRLPDNEIESKIFFISANERMAIFTNEVTAISAPKWDERNNEFASLFSYLQRKMSESADCKKFADLNNTYLEALANFNKCRCLIDEMKDKRIELAAKLKNELKKLEDFNSKINSEISAIFNDSYIDLLIKGKELLKKNPQLLNKNNSNEEKYPLLERETIQDQTVYGERLRLSENEFEELLHQIIENSILKLTKRIIELSQSANDMDSFMLNKIFDIYFSQYLNLSEQLSHFDVGSLENNLIEINRKIKIASGVTIGLTAAVTAAGIATGGAAFAIIGLAEAAIVGAGAAGGGAVAGGVLGSGAAFGAVKAVKLPFHFAWKENDLAIKNMWNSFESVLTDSYDRFRDNMTSNITEIITTIEAKAMKIRNNLNSIAEDLNNFPNRP 646

HSP 2 Score: 230.335 bits (586), Expect = 9.099e-67
Identity = 131/134 (97.76%), Postives = 132/134 (98.51%), Query Frame = 1 
Query:   64 MSHNGFESRNKIVNKLFKLTKDIYCDDDKNVSLFNLCKNYENDWNVGINVGILPDISERNICXXXXXXXXXXXXXXXXXVVLGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLVVNNEPR 465
            MSHNGFESRNKIVNKLFKLTKDIYCDDDKNVS FNLCKNYENDWNVGINVGILPDISERNICDDFDLLKSKLNNNDKKFVVLGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLVVNNE +
Sbjct:    1 MSHNGFESRNKIVNKLFKLTKDIYCDDDKNVSFFNLCKNYENDWNVGINVGILPDISERNICDDFDLLKSKLNNNDKKFVVLGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLVVNNEEK 134
[Close]
BLAST of Transmembrane GTPase Marf vs. Planmine SMEST
Match: SMESG000002973.1 (SMESG000002973.1)

HSP 1 Score: 67.3958 bits (163), Expect = 1.690e-11
Identity = 34/55 (61.82%), Postives = 42/55 (76.36%), Query Frame = 1 
Query:  301 VVLGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLVVNNEPR 465
            VV GKTSSGKS+LINA LTE +LPT  GSTSN F++IK S  S + Y+ +NNE +
Sbjct:   68 VVFGKTSSGKSSLINALLTEEILPTSCGSTSNSFVQIKAS-SSKKEYIRINNEVK 121
[Close]
BLAST of Transmembrane GTPase Marf vs. Planmine SMEST
Match: SMESG000002973.1 (SMESG000002973.1)

HSP 1 Score: 67.3958 bits (163), Expect = 2.062e-11
Identity = 37/69 (53.62%), Postives = 48/69 (69.57%), Query Frame = 1 
Query:  301 VVLGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLVVNNEPRY---KQEAKKILCD 498
            VV GKTSSGKS+LINA LTE +LPT  GSTSN F++IK S  S + Y+ +NNE +     +E   +LC+
Sbjct:   68 VVFGKTSSGKSSLINALLTEEILPTSCGSTSNSFVQIKAS-SSKKEYIRINNEVKTIESLRELTSLLCE 135
[Close]
BLAST of Transmembrane GTPase Marf vs. Planmine SMEST
Match: SMESG000002973.1 (SMESG000002973.1)

HSP 1 Score: 67.0106 bits (162), Expect = 2.685e-11
Identity = 34/55 (61.82%), Postives = 42/55 (76.36%), Query Frame = 1 
Query:  301 VVLGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLVVNNEPR 465
            VV GKTSSGKS+LINA LTE +LPT  GSTSN F++IK S  S + Y+ +NNE +
Sbjct:   68 VVFGKTSSGKSSLINALLTEEILPTSCGSTSNSFVQIKAS-SSKKEYIRINNEVK 121
[Close]
BLAST of Transmembrane GTPase Marf vs. Planmine SMEST
Match: SMESG000003238.1 (SMESG000003238.1)

HSP 1 Score: 57.7658 bits (138), Expect = 1.814e-8
Identity = 37/99 (37.37%), Postives = 56/99 (56.57%), Query Frame = 1 
Query:  301 VVLGKTSSGKSTLINAFLTENLLPTEFGSTSNCFIEIKGSLESNRNYLVVNNEPRYKQ---EAKKILCDILRLPDNEIESKIFF-----ISANERMAIF 573
            V  G+TS+GKS++IN F+ EN+LPT FGS SN  +EIK S ++ + +L V      KQ      K++     + +  I  K+FF     +  N+ M+IF
Sbjct:   69 VFYGRTSTGKSSIINRFIGENILPTGFGSVSNRIVEIKPS-QTTKTFLCVKENDTIKQTEIAPLKLIAKQNPIENFAIPIKVFFGYKQEVVINQNMSIF 166
The following BLAST results are available for this feature:
BLAST of Transmembrane GTPase Marf vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
MFN11.895e-640.00mitofusin 1 [Source:HGNC Symbol;Acc:HGNC:18262][more]
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BLAST of Transmembrane GTPase Marf vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
fzo-19.080e-743.64Transmembrane GTPase fzo-1 [Source:UniProtKB/Swis... [more]
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BLAST of Transmembrane GTPase Marf vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Marf2.211e-652.63gene:FBgn0029870 transcript:FBtr0343373[more]
Marf2.284e-652.63gene:FBgn0029870 transcript:FBtr0070910[more]
Marf2.284e-652.63gene:FBgn0029870 transcript:FBtr0070909[more]
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BLAST of Transmembrane GTPase Marf vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
MFN15.372e-738.36mitofusin 1a [Source:NCBI gene;Acc:100329462][more]
mfn1b3.217e-650.00mitofusin 1b [Source:ZFIN;Acc:ZDB-GENE-040426-1553... [more]
mfn23.584e-640.38mitofusin 2 [Source:ZFIN;Acc:ZDB-GENE-081105-44][more]
mfn1b3.644e-650.00mitofusin 1b [Source:ZFIN;Acc:ZDB-GENE-040426-1553... [more]
mfn1b3.744e-651.35mitofusin 1b [Source:ZFIN;Acc:ZDB-GENE-040426-1553... [more]
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BLAST of Transmembrane GTPase Marf vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mfn11.364e-646.81mitofusin 1 [Source:NCBI gene;Acc:548943][more]
mfn11.432e-646.81mitofusin 1 [Source:NCBI gene;Acc:548943][more]
mfn11.525e-646.81mitofusin 1 [Source:NCBI gene;Acc:548943][more]
mfn11.542e-646.81mitofusin 1 [Source:NCBI gene;Acc:548943][more]
mfn11.560e-646.81mitofusin 1 [Source:NCBI gene;Acc:548943][more]
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BLAST of Transmembrane GTPase Marf vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Mfn14.316e-744.68mitofusin 1 [Source:MGI Symbol;Acc:MGI:1914664][more]
Mfn18.564e-744.68mitofusin 1 [Source:MGI Symbol;Acc:MGI:1914664][more]
Mfn12.992e-644.68mitofusin 1 [Source:MGI Symbol;Acc:MGI:1914664][more]
Mfn12.992e-644.68mitofusin 1 [Source:MGI Symbol;Acc:MGI:1914664][more]
Mfn25.210e-651.35mitofusin 2 [Source:MGI Symbol;Acc:MGI:2442230][more]
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BLAST of Transmembrane GTPase Marf vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transmembrane GTPase Marf vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transmembrane GTPase Marf vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
ENSAMXT00000043809.14.488e-743.64mitofusin-1-like [Source:NCBI gene;Acc:103034819][more]
mfn1b2.311e-647.83mitofusin-1 [Source:NCBI gene;Acc:103030239][more]
mfn1b2.401e-647.83mitofusin-1 [Source:NCBI gene;Acc:103030239][more]
mfn24.274e-638.60mitofusin 2 [Source:ZFIN;Acc:ZDB-GENE-081105-44][more]
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BLAST of Transmembrane GTPase Marf vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transmembrane GTPase Marf vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transmembrane GTPase Marf vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO423791.778e-639.62Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Transmembrane GTPase Marf vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 4
Match NameE-valueIdentityDescription
mfn1b5.504e-747.83mitofusin 1 [Source:NCBI gene;Acc:101158009][more]
mfn1b6.226e-747.83mitofusin 1 [Source:NCBI gene;Acc:101158009][more]
ENSORLT00000009003.28.734e-731.78pep primary_assembly:ASM223467v1:17:11595384:11602... [more]
mfn21.480e-650.00mitofusin 2 [Source:NCBI gene;Acc:101170481][more]
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BLAST of Transmembrane GTPase Marf vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000003246.16.882e-17884.25SMESG000003246.1[more]
SMESG000002973.11.690e-1161.82SMESG000002973.1[more]
SMESG000002973.12.062e-1153.62SMESG000002973.1[more]
SMESG000002973.12.685e-1161.82SMESG000002973.1[more]
SMESG000003238.11.814e-837.37SMESG000003238.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015381 ID=SMED30015381|Name=Transmembrane GTPase Marf|organism=Schmidtea mediterranea sexual|type=transcript|length=1825bp
TAAACTGATTTTGAAATAATACAAATTATATAATTTATTTACTTGTATCG
CGAATAATTTATAATGTCGCATAATGGATTTGAAAGCCGAAATAAAATTG
TAAATAAACTATTCAAATTAACGAAAGATATTTATTGTGATGATGATAAA
AATGTTTCCTTATTTAATTTATGTAAAAATTATGAAAACGACTGGAATGT
TGGAATTAATGTTGGAATTTTACCAGATATATCCGAAAGAAACATATGTG
ATGATTTTGATTTGTTGAAGTCAAAATTGAATAATAATGATAAAAAATTC
GTGGTTTTAGGAAAAACTTCATCGGGGAAATCAACTCTTATAAATGCTTT
TTTGACAGAAAATTTGTTACCGACTGAGTTTGGGTCGACGTCTAATTGTT
TCATTGAAATTAAGGGTAGCCTTGAGAGCAATAGAAATTACCTCGTGGTC
AATAATGAACCCAGATATAAACAAGAAGCTAAAAAAATTCTTTGCGATAT
TTTGCGTCTTCCTGACAATGAAATTGAGTCGAAAATATTTTTCATTTCCG
CTAACGAAAGAATGGCTATATTTACTAATGAAGTGACGGCGATTAGTGCT
CCGAAATGGGATGAACGCAACAACGAATTTGCTAGTCTTTTCTCATATTT
GCAGACAAAGATGAGCGAAAGTGCCGATTGCAAGAAATTTGCTGACTTGA
ATAATACATATCTTGAAACGCTTGCCAATTTTAATAAATGCCGATGTTTA
ATAGACGAAATGAAAGATAAAAGGATAGAACTAGCTGCCAAATTAAAAAA
TGAGCTAAAGAAATTAGAGGATTTCAATTCTAAAATTAATTCAGAAATTT
CCGCAATATTTAATGACTCGTACACTGACTTACTTATAAAAGGAAAGGAA
CTTTTGAAAAAAAACCCTCAACTTTTAAATAAAAATAATTCTAACGAAGA
AAAGTATTCTCTATTAGAACACGAGACTATACAAGACCAAACGGTGTACG
GAGAAGGATTAAGATTGAGTGAAAACGAATTTGAGGAATTATTGCATCAA
ATAATCGAGAATTCTATTCTTAAGTTAACAAAAAGAATCATTGAACTTTC
TCAATCTGCGGATGACATGGATTCATTCATGCTAAATAAAATTTTCGACA
TATATTTTTCACAATATTTGAACTTGAGTGAGCAACTGAGTCATTTTGAT
GTCGGATCTTTGGAAAACAATCTCATCGAAATATTTGCTGTTATCGGATT
AGCGGATGCAGTCATATTTGCTGGTAGACTGGTGGGCGGAGCTGTGGCTG
GTGGTGTTATTGGATTTGGTGCAGCTTTGGGTGCTGTTAAAGCTCTGAAA
TTGCCTTTTCCTTTAGCATGGAAAAAAAATCACTTGGCTATCAGAAGCAT
GTGGAATTCATTCGAGTCGGTATTGACCGACAGCTACAACAGATTCCGTG
ATAATATGACATCCAACATCACAGAAATTATTACAACAATTGAAGCAAAA
GCAATGAAAATTCGAAACAATTTGAACAGTATAGCTGAAGATTTGAACAA
TTTCCCGAATAGGCCATAGGCCAAAGAAAATGTCCATCGTCTGCCAATAT
AAAGTGATACCTGGCAATACTTTAAATTTACTTCACAACTGTAATAATCT
ATTTTATATTTTTTTAAATTCCAAGTTCTCTTCTCAATTACAGGAAATTA
ATTTTTTTGTAATTTGGTTTACTTTATGAATGGTTATTAATGAGTAGCAA
CACAGGAGTCATTACCATGGCTCAATCAAAACAGTTACACGGGTTTCAAC
TGAATAGCAACTCCAGATCGGAAGA
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protein sequence of SMED30015381-orf-1

>SMED30015381-orf-1 ID=SMED30015381-orf-1|Name=SMED30015381-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=502bp
MSHNGFESRNKIVNKLFKLTKDIYCDDDKNVSLFNLCKNYENDWNVGINV
GILPDISERNICDDFDLLKSKLNNNDKKFVVLGKTSSGKSTLINAFLTEN
LLPTEFGSTSNCFIEIKGSLESNRNYLVVNNEPRYKQEAKKILCDILRLP
DNEIESKIFFISANERMAIFTNEVTAISAPKWDERNNEFASLFSYLQTKM
SESADCKKFADLNNTYLETLANFNKCRCLIDEMKDKRIELAAKLKNELKK
LEDFNSKINSEISAIFNDSYTDLLIKGKELLKKNPQLLNKNNSNEEKYSL
LEHETIQDQTVYGEGLRLSENEFEELLHQIIENSILKLTKRIIELSQSAD
DMDSFMLNKIFDIYFSQYLNLSEQLSHFDVGSLENNLIEIFAVIGLADAV
IFAGRLVGGAVAGGVIGFGAALGAVKALKLPFPLAWKKNHLAIRSMWNSF
ESVLTDSYNRFRDNMTSNITEIITTIEAKAMKIRNNLNSIAEDLNNFPNR
P*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0005739mitochondrion
GO:0005741mitochondrial outer membrane
GO:0016021integral component of membrane
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR027094Mitofusin_fam
IPR022812Dynamin_SF
IPR027417P-loop_NTPase
Vocabulary: molecular function
TermDefinition
GO:0003924GTPase activity
GO:0005525GTP binding
Vocabulary: biological process
TermDefinition
GO:0008053mitochondrial fusion
GO:0048662negative regulation of smooth muscle cell proliferation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 230..261
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 80..264
e-value: 2.3E-8
score: 35.8
NoneNo IPR availablePANTHERPTHR10465:SF0TRANSMEMBRANE GTPASE MARF-RELATEDcoord: 68..121
IPR022812Dynamin superfamilyPFAMPF00350Dynamin_Ncoord: 80..141
e-value: 6.6E-8
score: 32.8
IPR027094Mitofusin familyPANTHERPTHR10465TRANSMEMBRANE GTPASE FZO1coord: 68..121
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 66..128