G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30015371
Length (bp)1438
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30015371) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30015371) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30015371) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30015371) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30015371

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30015371h1SMcG0001878 SmedASXL_004808SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30015371h1SMcG0001878 SmedASXL_004809SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
non-ciliated neuronSMED30015371h1SMcG0001878 dd_Smed_v4_35525_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30015371h1SMcG0001878 dd_Smed_v6_35525_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Match: dmsr-7 (DroMyoSuppressin Receptor related [Source:UniProtKB/TrEMBL;Acc:O16548])

HSP 1 Score: 53.1434 bits (126), Expect = 2.809e-7
Identity = 50/194 (25.77%), Postives = 81/194 (41.75%), Query Frame = 2 
Query:  719 WLDGLLYSYLPFFLILCLNGLIVSHVRKAREHRNILVSRCPQSNYAQIKDCSVRNLSHSVNTQAGSRQTFQLTLMLFSISACFLITTCPIVVMKIVNFTQNRDTLDQRGSALHQLLDSIAELLMYLNHSANFYLYCATGSKFRNELKSLC--------------SDTRATRSRLQNQTTLAKSRVHQPYPVEKD 1258
            W+ G+L+  +P  L++ L+  +VS +R A +HR  L S  P +     K    +N          S +T   TLML  I   FLIT  P  ++ I+      D        L+  +  + +LL  +N S NF LYC   S++R     +               S T  ++ +L N   L + + + P   E +
Sbjct:  227 WMIGILFKIIPCILLIFLSFGLVSKIRDAEKHRRKLTS-VPSNASTDSKPLKKKN--------GTSDRT---TLMLVVILLVFLITEFPQGIISILCAIFTTDV----HRYLYFYIGDVLDLLSLVNSSVNFVLYCVMSSRYRQTFWEVIIPSWACGLWTTRRGSPTELSQLQLNNSLRLGRKQSYTPLATEPE 404
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A2T7NJU4 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_19599 PE=3 SV=1)

HSP 1 Score: 174.481 bits (441), Expect = 6.568e-46
Identity = 116/319 (36.36%), Postives = 171/319 (53.61%), Query Frame = 2 
Query:  236 TIGNCTSFMILTKR---RVSSYVYLSALAMADQGVLITGLMRHCIDRILDYRLEDSHHWTCVLTQLIGSSLSYLSAWLIVAVTAERAIVIMFPFSSIRLYSRFRETXXXXXXXXXXXXXXXHFLLSVRLETGQMYRFNLNNGSVQNYSQCQLRNEL-HSFSKAWYWLDGLLYSYLPFFLILCLNGLIVSHVRKAREHRNILVSRCPQSNYAQIKDCSVRNLSHSVNTQA-----GSRQTFQLTLMLFSISACFLITTCPIVVMKIVNFTQNRDTLDQRGSALHQLLDSIAELLMYLNHSANFYLYCATGSKFRNELKSL 1165
            T GN  SF++L +R   +VSSY+YL++LA+AD  VL  GL+R  +D +     +D+  W C +T  +G   S LS WLI+AVT ER IV+ FP  +  + +  R  LV++ I       + HF  +V    G + R   ++    N + C+      H     W W+D  +YS+LPF +IL  N LI++ V +AR HR + +   P  +Y Q K    R  +             S +  +LT+ML ++S  FL+TT P+ +  IV     +     R  A  +L  +IAELLMY+NHS NF+LYCATG KFR ++  L
Sbjct:   35 TFGNVVSFIVLRRRAMSKVSSYLYLASLAIADSLVLYIGLLRLWLDELTGVNFQDNSEWLCKVTMFLGYLASDLSVWLIIAVTVERYIVVCFPLRAFTMCNTNRAKLVIIFIVFLICCMNMHFFWTV----GLVERATDSD----NITICEAAPAYNHLVDDVWPWVDACVYSFLPFVIILVCNVLIITEVVEARAHR-LNLQNMPDYHYHQQKQLQHRTSTTGTAISGCRRSCASGEGTKLTVMLLTVSFTFLLTTLPMNISLIVTAFWEQQNHGIRQIAQLKLAKTIAELLMYVNHSINFFLYCATGQKFRQQIAHL 344
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0K0PUW3 (Orphan G-protein coupled receptor 57 OS=Platynereis dumerilii OX=6359 PE=2 SV=1)

HSP 1 Score: 173.326 bits (438), Expect = 5.692e-45
Identity = 113/314 (35.99%), Postives = 171/314 (54.46%), Query Frame = 2 
Query:  236 TIGNCTSFMILTKR---RVSSYVYLSALAMADQGVLITGLMRHCIDRILDYRLEDSHHWTCVLTQLIGSSLSYLSAWLIVAVTAERAIVIMFPFSSIRLYSRFRETXXXXXXXXXXXXXXXHFLLSVRLETGQMYRFNLNNGSVQNYSQCQ-LRNELHSFSKAWYWLDGLLYSYLPFFLILCLNGLIVSHVRKAREHRNILVSRCPQSNYAQIKDCSVRNLSHSVNTQAGSRQTFQLTLMLFSISACFLITTCPIVVMKIVNFTQNRDTLDQRGSALHQLLDSIAELLMYLNHSANFYLYCATGSKFRNELKSL 1165
            T GN  SF+IL +R   + S+Y+YL+ L++ D  VL  GL+R  I  +    + D   W C +  ++G ++S  S WLI+AVT ER + + +P  +  + +R R  +V++ + LA L  + HFL      T Q+  +N+N   +    QC+   N      + W W+D  LYS+LPF +I  LN LI+  V  AR +R+ L S  P +  ++ ++ S++                 LT+ML +IS  FL+TT P+ +  I     N+   D       +L+ +I ELLMY+NHS NFYLYCATG KFR+EL SL
Sbjct:   80 TFGNIFSFIILMRRSMRKFSTYIYLAMLSLTDTLVLYIGLLRLWIGELTGMDIRDKSDWICKVINVVGYTVSDYSVWLIIAVTVERYVAVCYPLRAPAMCNRRRAIVVIVCLLLALLFINLHFLW-----TAQIMEYNINGDIIY---QCEGAPNHTRLVQEVWPWVDAFLYSFLPFVVISVLNALIIRQVAHARRNRSGLQSNMPDTR-SRAQESSIK-----------------LTIMLLTISFAFLLTTLPMNIALIGTAFYNQYHKDLHIMTKFKLIRTITELLMYVNHSMNFYLYCATGQKFRHELVSL 367
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A2H1CXP9 (7 transmembrane receptor OS=Fasciola hepatica OX=6192 GN=D915_000019 PE=4 SV=1)

HSP 1 Score: 173.326 bits (438), Expect = 7.949e-44
Identity = 122/346 (35.26%), Postives = 185/346 (53.47%), Query Frame = 2 
Query:  236 TIGNCTSFMIL---TKRRVSSYVYLSALAMADQGVLITGLMRHCIDRILDYRLEDSHHWTCVLTQLIGSSLSYLSAWLIVAVTAERAIVIMFPFSSIRLYSRFRETXXXXXXXXXXXXXXXHFLLSVRL---------ETGQMYRFNLNNGSV--QNYSQ---------------CQLRNELHSFSKAWYWLDGLLYSYLPFFLILCLNGLIVSHVRKAREHRNILVSRCPQSNYAQIKDCSVRNLSHSVNTQAGSRQTF----QLTLMLFSISACFLITTCPIVVMKIVNFTQNRDTLDQRGSALH------QLLDSIAELLMYLNHSANFYLYCATGSKFRNEL 1156
            + GN  SF++L   + R VS++VYL+  ++ D+ VLI GL+R  ID +++++LED+    C + Q  G S S+LS WL V +TAERA+V+ FP    R  +  R   ++  +++ C+L S+HF ++V L         +    Y     N +V  +NYSQ               C  + E  + +  W  +D ++Y YLPF LI  LN +IV H   A++ ++ +  R   ++  Q+             T + SR+      Q+T+ML  IS  FLITT  IVV KI++       LD  GS+        QL DS+AELLMY+NH+ NFYL+CATG +FR+ L
Sbjct:   81 SFGNILSFLVLRHPSTRYVSAFVYLTVRSVVDEVVLIVGLLRRWIDYLINHKLEDTSSTLCKVAQFAGVSSSFLSVWLTVTLTAERALVVSFPLHGTRFITYSRVRRLIFTLTVICVLLSTHFFVTVDLIPIPGSNKAKKEHTYSEPWANRTVLRRNYSQHELSTIKPTSEQVYRCNFKPEFAALAWLWTTMDAVIYCYLPFILIFGLNVVIVHHANAAQKEQHRIRVRHSTTSKQQLG-----------KTPSFSREQVNNVRQMTIMLLVISFAFLITTLAIVVTKILS-----QVLDLSGSSRRLQRLNFQLADSVAELLMYINHAMNFYLFCATGKRFRSRL 410
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A504YW10 (G-protein coupled receptor OS=Fasciola gigantica OX=46835 GN=FGIG_06654 PE=4 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 3.231e-43
Identity = 119/345 (34.49%), Postives = 181/345 (52.46%), Query Frame = 2 
Query:  236 TIGNCTSFMIL---TKRRVSSYVYLSALAMADQGVLITGLMRHCIDRILDYRLEDSHHWTCVLTQLIGSSLSYLSAWLIVAVTAERAIVIMFPFSSIRLYSRFRETXXXXXXXXXXXXXXXHFLLSVRL-------------ETGQMYRFNLNNGSVQ-NYSQ---------------CQLRNELHSFSKAWYWLDGLLYSYLPFFLILCLNGLIVSHVRKAREHRNILVSRCPQSNYAQIKDCSVRNLSHSVNTQAGSRQTFQLTLMLFSISACFLITTCPIVVMKIVNFTQNRDTLDQRGSALH------QLLDSIAELLMYLNHSANFYLYCATGSKFRNEL 1156
            + GN  SF++L   + R VS++VYL+  ++ D+ VLI GL+R  ID +++ +LED+    C + Q  G S S+LS WL V +TAERA+V+ FP    R  +  R   ++  +++ C+L S+HF ++V L                +++  + N   +Q NYSQ               C  + E  + +  W  LD ++Y YLPF  I  LN +IV H   A++ ++ +  R    +  Q+      +     N +       Q+T+ML  IS  FLITT  IVV KI++       LD  GS+        QL DS+AELLMY+NH+ NFYL+CATG +FR+ L
Sbjct:   81 SFGNILSFLVLRHPSTRYVSAFVYLTVRSVVDEVVLIVGLLRRWIDYLINRKLEDTSSTLCKMAQFAGVSSSFLSVWLTVTLTAERALVVSFPLHGTRFITYSRVRRLIFTLTVICVLLSTHFFITVDLIPIPGSTKAKKEHAYSELWEQDANRTVLQRNYSQPELSTIMPTSEQVYRCNFKPEFAALAWLWTTLDAVIYCYLPFIFIFGLNVVIVHHANAAQKEQHRIRVRHSTISMQQLGKTPSFSREQVNNVR-------QMTIMLLVISFAFLITTLAIVVTKILS-----QVLDLSGSSRRLQRLNFQLADSVAELLMYINHAMNFYLFCATGKRFRSRL 413
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183A9A8 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS3543 PE=4 SV=1)

HSP 1 Score: 168.703 bits (426), Expect = 4.349e-43
Identity = 128/387 (33.07%), Postives = 188/387 (48.58%), Query Frame = 2 
Query:  236 TIGNCTSFMIL---TKRRVSSYVYLSALAMADQGVLITGLMRHCIDRILDYRLEDSHHWTCVLTQLIGSSLSYLSAWLIVAVTAERAIVIMFPFSSIRLYSRFRETXXXXXXXXXXXXXXXHFLLSVRL-------------------------------ETGQMYRFNLNNGSVQNYS--------QCQLRNELHSFSKAWYWLDGLLYSYLPFFLILCLNGLIVSHVRKAREHRNILVSRCPQSNYAQIKDCSVRNLSHSVNTQAGSRQTFQLTLMLFSISACFLITTCPIVVMKIVNFTQNRDTLDQRGSA------LHQLLDSIAELLMYLNHSANFYLYCATGSKFRNELKSLCSDTRATRSRL----QNQTTLAKSRVHQP 1240
            + GN  +FM+L   + R VS++VYL+  ++ D+ VL  GL+R  ID +++ +LE S    C L Q  G S S+LS WL V +TAERA+V+ FP    R  S  R   +++++SL C+L S HF ++V L                                T + +R    +    + S        QC  + E  SF+  W  +D ++Y YLPF LI  LN +IV HV  A++ ++ +  R       Q+   +  +     N +       Q+T+ML  +S  FL+TT  IVV KI++       LD  GS         QL D++AELLMY+NH+ NFYL+CATG +FR+ L  L +  R T        Q QT       H P
Sbjct:   81 SFGNILAFMVLRHPSTRYVSAFVYLTVRSVVDEVVLTVGLLRRWIDYMINRKLEHSTSVFCKLAQFAGVSSSFLSVWLTVTLTAERALVVSFPLHGTRFISYSRVRKLIIVLSLICILLSLHFFITVDLIPTTTTPSSSTATTDFIHQPYHSLYLEPQDSNTTEPWRNQTQSAITGDVSSVRQEMVYQCNFKPEFASFAWLWTTIDAVVYCYLPFILIFGLNVVIVYHVNAAQKKQHRIRVRHSVIPMQQLSKATGASGDQMNNVR-------QMTIMLLVVSFAFLVTTVAIVVTKILS-----QVLDLSGSGRTHERLYFQLADTVAELLMYINHAMNFYLFCATGKRFRSRLWYLLAKCRPTAPNRGTADQQQTARLDPDCHYP 455
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000035261.1 (probable G-protein coupled receptor 139 [Source:NCBI gene;Acc:111191700])

HSP 1 Score: 63.929 bits (154), Expect = 1.209e-10
Identity = 79/296 (26.69%), Postives = 127/296 (42.91%), Query Frame = 2 
Query:  284 SSYVYLSALAMADQGVLI-TGLMRHCIDRILDYRLEDSHHWTCVLTQLIGSSLSYLSAWLIVAVTAERAIVIMFPFSSIR-LYSRFRETXXXXXXXXXXXXXXXHFLLSVRLETGQMYRFNLN-NGSVQNYSQCQLRNELHSFSKAWYWLDGLLYSYLPFFLILCLNGLIVSHVRKAREHRNILVSRCPQSNYAQIKDCSVRNLSHSVNTQAGSRQTFQLTLMLFSISACFLITTCP-IVVMKIVNFTQNRDTLDQRGSALH-QLLDSIAELLMYLNHSANFYLYCATGSKFRNEL 1156
            S+ +YLS LA+ D   L+   L+  C+  +        H W C +  ++     Y SAW++VA T ER +V+    S  R L++R        +I L+ L +     ++V      + + NL  NG V +  +C  R  L  +S    W+   L S +P  L++  N LI  H+             C    + + +    R       T+   R+T    L+L +IS  F++ T P  V   I+    N    D+    +   ++  +A +L  LN S NF LYC    +FR EL
Sbjct:   43 SARIYLSCLALVDTCYLVWVILLDLCLTFLQSQPFWHYHPW-CGIMNILQFGTFYCSAWVVVAFTIERFLVLRATVSCQRPLHARHALLTCTTIILLSHLASIPAGWINV------VAQVNLTLNGQVVSMPRCIYREAL--YSTVVVWITSFLSSGVPIMLVIIFNSLIAYHLL------------CSSRLFTKEE----RRTIQGSKTRGSIRRTL---LLLGTISVAFVVLTLPRFVTYCILRTIYNHKDFDRDDYEMPINIVSDVANMLHNLNSSTNFLLYCMVSRRFRQEL 310
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000042752.1 (pep primary_assembly:ASM223467v1:24:10229220:10232247:1 gene:ENSORLG00000028764.1 transcript:ENSORLT00000042752.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 54.6842 bits (130), Expect = 9.934e-8
Identity = 80/301 (26.58%), Postives = 134/301 (44.52%), Query Frame = 2 
Query:  284 SSYVYLSALAMADQGVLITGLMRHCIDRILDYRLED----SHHWTCVLTQLIGSSLSYLSAWLIVAVTAERAIVIMFPFSSIRLYSRFRETXXXXXXXXXXXXXXXHFLLSVRLETGQMYRFNLNN----GSVQNYSQCQLRNELHSFSKAWYWLDGLLYSYLPFFLILCLNGLIVSHVRKAREHRNILVSRCPQSNYAQIKDCSVRNLSHSVNTQAGSRQTFQLTLMLFSISACFLITTCP-IVVMKIVNFTQNRDTLDQRGSALH-QLLDSIAELLMYLNHSANFYLYCATGSKFRNEL 1156
            ++ VYLS+LA+AD   LI  ++   +D  L + +      S  W  +L  L   SL   S+W++V  T ER +V+     S  +  R  E  V  L  +A ++ S   L+SV +  G +    L N    G      +C  R   H++S A  W+   L   +P  L++  N LI  ++             C  SN    ++   + + H  +T+   ++T     +L +IS  F++ + P  V   I+    N ++ D+   ++   +   +A +L  LN S NF LYC    +FR EL
Sbjct:   45 AAVVYLSSLAIADTFYLIWVIL---LDLTLTFWILQPFWHSQPWCGILGFLQHGSLCS-SSWIVVVFTIERYLVL----KSSTVKQRLSEAGVTKLTCMAIVVLSH--LVSVPM--GWINTVKLVNLTVDGENMTLPRCLYRE--HTYSTAVVWIASFLSGGIPIVLVILFNYLIGYNL-------------CRASNLFTKEE---QRVMHGRSTRGMLKRTI---FLLGTISVVFVVLSLPRFVTYCILRTKHNDESFDRNDYSIPINVAGDVANMLHNLNSSTNFLLYCVVSHRFRQEL 312
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000075029.1 (SMESG000075029.1)

HSP 1 Score: 674.47 bits (1739), Expect = 0.000e+0
Identity = 344/350 (98.29%), Postives = 347/350 (99.14%), Query Frame = 2 
Query:  236 TIGNCTSFMILTKRRVSSYVYLSALAMADQGVLITGLMRHCIDRILDYRLEDSHHWTCVLTQLIGSSLSYLSAWLIVAVTAERAIVIMFPFSSIRLYSRFRETXXXXXXXXXXXXXXXHFLLSVRLETGQMYRFNLNNGSVQNYSQCQLRNELHSFSKAWYWLDGLLYSYLPFFLILCLNGLIVSHVRKAREHRNILVSRCPQSNYAQIKDCSVRNLSHSVNTQAGSRQTFQLTLMLFSISACFLITTCPIVVMKIVNFTQNRDTLDQRGSALHQLLDSIAELLMYLNHSANFYLYCATGSKFRNELKSLCSDTRATRSRLQNQTTLAKSRVHQPYPVEKDLSNS*FLKR 1285
            TIGNCTSF+ILTKRRVSSYVYLSALAMADQGVLITGLMRHCIDRILDYRLEDSHHWTCVLTQLIGSSLSYLSAWLIVAVTAERAIVIMFPFSSIRLYSRFRETLVVLLISLACLLASSHFLLSVRLETGQMYRFNLNNGSVQNYSQCQLRNELHSFSKAWYWLDGLLYSYLPFFLILCLNGLIVSHVRKAREHRNILVSRCPQSNYAQIKDCSVRNLSHSVNTQAGSRQTFQLTLMLFSISACFLITTCPIVVMKIVNFTQNRDTLDQRGSALHQLLDSIAELLMYLNHSANFYLYCATGSKFRNELKSLCSDTRATRSRLQNQTTLAKSRVHQPYPVEKDLSN  F+ +
Sbjct:   94 TIGNCTSFIILTKRRVSSYVYLSALAMADQGVLITGLMRHCIDRILDYRLEDSHHWTCVLTQLIGSSLSYLSAWLIVAVTAERAIVIMFPFSSIRLYSRFRETLVVLLISLACLLASSHFLLSVRLETGQMYRFNLNNGSVQNYSQCQLRNELHSFSKAWYWLDGLLYSYLPFFLILCLNGLIVSHVRKAREHRNILVSRCPQSNYAQIKDCSVRNLSHSVNTQAGSRQTFQLTLMLFSISACFLITTCPIVVMKIVNFTQNRDTLDQRGSALHQLLDSIAELLMYLNHSANFYLYCATGSKFRNELKSLCSDTRATRSRLQNQTTLAKSRVHQPYPVEKDLSNMIFMIK 443

HSP 2 Score: 157.918 bits (398), Expect = 6.546e-43
Identity = 76/76 (100.00%), Postives = 76/76 (100.00%), Query Frame = 3 
Query:    6 MPKVCDHVDLGSVSILRSFLSNISTENQTMNILNLQKTRVFINDTVNWESLLNNYRLQLSKAIEKASPGSELMTCN 233
            MPKVCDHVDLGSVSILRSFLSNISTENQTMNILNLQKTRVFINDTVNWESLLNNYRLQLSKAIEKASPGSELMTCN
Sbjct:    1 MPKVCDHVDLGSVSILRSFLSNISTENQTMNILNLQKTRVFINDTVNWESLLNNYRLQLSKAIEKASPGSELMTCN 76
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000020718.1 (SMESG000020718.1)

HSP 1 Score: 191.815 bits (486), Expect = 2.127e-55
Identity = 122/331 (36.86%), Postives = 179/331 (54.08%), Query Frame = 2 
Query:  236 TIGNCTSFMIL---TKRRVSSYVYLSALAMADQGVLITGLMRHCIDRILDYRLEDSHHWTCVLTQLIGSSLSYLSAWLIVAVTAERAIVIMFPFSSIRLYSRFRETXXXXXXXXXXXXXXXHFLLSVRL-ETGQMYRFNLNNGSVQNYSQCQLRNELHSFSKAWYWLDGLLYSYLPFFLILCLNGLIVSHVRKAREHRNILVSRCPQSNYAQIKDCSVRNLSHSVNTQAGSRQTFQLTLMLFSISACFLITTCPIVVMKIV---------NFTQNRDTLDQRGSALHQLLDSIAELLMYLNHSANFYLYCATGSKFRNELKSLCSDTRATR 1189
            TIGN  SF++L     + VS+++YLS LA+ DQ VLI GL++  +D +L  ++E+ +   C+L+Q  G+S S LS WLIVAVT ERAIVI FPF +  L  + R  ++ + ++   ++ + HF+ ++ L  + Q++       S  N   C       +F   W  +D ++YS+LPF  I  LN +I+  V +AR  R                  S+  L+    +   S  T QLT+ML  IS  FLITT PI ++K++            +       R +   Q  DSIAE+LM+LNH+ NFYLYCA+G+KFR  LKSL S  R  R
Sbjct:   96 TIGNVMSFIVLCNFAGKTVSTFIYLSLLAIVDQFVLIFGLLKRWVDYLLKRKIENRNKTFCILSQYFGTSASVLSVWLIVAVTLERAIVISFPFKAHNLIQKSRAIVISISMASVIMILNCHFIFTLDLTNSTQLFE------STDNNFVCGFSESYRNFKTVWLLIDAIIYSFLPFIFIFALNVIILIKVCQARRTRQ-----------------SMMTLNRGQKSVYRSESTRQLTIMLLMISGTFLITTTPIFIVKMLYVVPKAFSKAIPKTFHAPGIRDAVASQFADSIAEILMHLNHALNFYLYCASGNKFRACLKSLFSKCRLFR 403
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000042142.1 (SMESG000042142.1)

HSP 1 Score: 177.563 bits (449), Expect = 4.698e-50
Identity = 118/351 (33.62%), Postives = 190/351 (54.13%), Query Frame = 2 
Query:  236 TIGNCTSFMILTKR---RVSSYVYLSALAMADQGVLITGLMRHCIDRILDYRLEDSHHWTCVLTQLIGSSLSYLSAWLIVAVTAERAIVIMFPFSSIRLYSRFRETXXXXXXXXXXXXXXXHFLLSVRLETGQMYRFNLNNGSVQ---NYSQCQLRNELHSFSKAWYWLDGLLYSYLPFFLILCLNGLIVSHVRKAREHRNILVSRCPQSNYA------------QIKDCSVRNLSHSVNTQAGSRQTFQLTLMLFSISACFLITTCPIVVMKIVNFTQNRDTLDQRGSALHQLLDSIAELLMYLNHSANFYLYCATGSKFRNELKSLCSDTRATRSRLQNQTTLAKSRVH 1234
            +IGN  S ++L ++    +SSY+YL+ LA+ D+ +L+ GL R  I+ + D+ LE+     C L Q+IG S S++S WLIV +T ER ++I  PF + +     R   +++ + +     S HF  +V L+       N N  S +   +  +CQ + E   F+ AW+++DG  YS++P FL++  N +I+S + KAR   N +++     N              ++K+ S RN           R+T QLT+ML  +S  FLITT P V++K++   +     D++ + L  LLD I E+LMY+NH+ NFYLYCATG+KFR +L  +    R+  S+    T     + H
Sbjct:   83 SIGNFLSAVVLCRKSMSHISSYIYLATLAIFDELILLCGLFRKWIEHVTDWSLENHSISFCKLFQVIGVSSSFISVWLIVTLTVERVLIIKRPFEASKYAKASRAKYIIVSLIIISFTTSCHFFETVTLQNLNHKNVNTNFSSDKGSNSIKKCQFKTEYTKFTNAWFFIDGAFYSWIPLFLLIINNLIILSEIYKARNIHNSMINTNHDENKTLGTSIVRTQLPRRMKESSSRN-----------RETKQLTVMLLIVSFSFLITTSPFVILKLLIKLKGYPNGDRKLAQL-VLLDGIFEMLMYVNHAINFYLYCATGNKFRQQLIKILFCRRS--SKFSTTTNFKTGKYH 419
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000053893.1 (SMESG000053893.1)

HSP 1 Score: 174.096 bits (440), Expect = 3.882e-49
Identity = 117/320 (36.56%), Postives = 181/320 (56.56%), Query Frame = 2 
Query:  236 TIGNCTSFMILTKR---RVSSYVYLSALAMADQGVLITGLMRHCIDRILDYRLEDSHHWTCVLTQLIGSSLSYLSAWLIVAVTAERAIVIMFPFSSIRLYSRFRETXXXXXXXXXXXXXXXHFLLSVRLE----------TGQMYRFNLNNGSVQNYSQCQLRNELHSFSKAWYWLDGLLYSYLPFFLILCLNGLIVSHVRKAREHRNILVSRCPQSNYAQIKDCSVRNLSHSVNTQAGSRQTFQLTLMLFSISACFLITTCPIVVMKIVNFTQNRDTLDQRGSALHQLLDSIAELLMYLNHSANFYLYCATGSKFRNEL 1156
            T GN  +F ++ +R   + SSYVYL+A+A+ D+ +L+TGL+R   D ++  R+EDS+ ++C + Q IG ++S+ S WLIV +T ER I+I  P    RL S  +  + ++++     L SSHF  +V L           T  +YR             C  +++ + F + W   + L YS +PF L++  NG+I+S   KA + R+ LV +C  +   Q +  S        N    + +T QLT+ML +IS  FL TT P+V  K++    N +T D R  A+ QL+D+I ELLMY+NHS NFY+YCA+G +FR +L
Sbjct:   85 TFGNILAFFVICQRHLLKTSSYVYLAAIAIIDELILLTGLLRRWSDHLVGSRIEDSNKFSCKIFQFIGVTVSFSSVWLIVFMTVERLIIIKLPLKVNRLVSPNKVKVYIVILIFCLSLLSSHFFETVSLNNQNNSNSNLVTTPLYR-------------CNFKDKYYHFIRYWMMFEALFYSIIPFILLIIFNGIILSLTYKASKTRSTLVKQCYMTR--QFQSISHNRQKVVTNVSQLNHETKQLTVMLVTISVAFLCTTTPLVTYKLILKVIN-NTDDVRYLAIRQLMDTIFELLMYINHSINFYIYCASGRRFRTQL 388
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000074092.1 (SMESG000074092.1)

HSP 1 Score: 168.318 bits (425), Expect = 5.072e-47
Identity = 114/344 (33.14%), Postives = 180/344 (52.33%), Query Frame = 2 
Query:  236 TIGNCTSFMIL---TKRRVSSYVYLSALAMADQGVLITGLMRHCIDRILDYRLEDSHHWTCVLTQLIGSSLSYLSAWLIVAVTAERAIVIMFPFSSIRLYSRFRETXXXXXXXXXXXXXXX--HFLLSVRLETGQMYRFNLNNGSVQNYSQCQLRNELHSFSKAWYWLDGLLYSYLPFFLILCLNGLIVSHVRKAREHRNIL-----VSRCPQSNYAQ------------------IKDCSVRNLSHSVNTQAGSRQTFQLTLMLFSISACFLITTCPIVVMKIVNFTQNRDTLDQRGSALHQLLDSIAELLMYLNHSANFYLYCATGSKFRNELKSLCSDTRA 1183
            T+GN  SF +L   ++  +S Y Y   L + DQ +L+ GL+R  I      R+++ +   C   Q + SSLS++S WL++ +T  RA VI +PFS  +L  +F++    LLI +         HF  +V + +      ++NN S+   S+C+   + H FS+ W W+D  +Y Y P F+I   N  IVS++ K+R++R  L     + +   +N +Q                  I DC+   L H             ++ ML ++S CF+ITT PIV++K ++   N+  +D       +LL+S AELLMYLNH  NFYLY  TGSKFR ++K+L ++ R 
Sbjct:   97 TVGNILSFAVLFQKSRNNLSYYSYFCLLTVVDQLILLIGLLRKWIFYFDIDRIDNKNSLVCAFLQCLNSSLSWVSTWLLMLITVYRAFVISYPFS--KLNEKFQKKCKFLLILITSSALLLNCHFFFTVEIVSVP----SINNESL---SKCKYVKKYHGFSEIWSWIDASIYCYFPSFVICISNVFIVSNIVKSRKYRLKLGETRTLPKIQTTNRSQPLNEDSNGLLGKSKIKIKISDCNNGRLIH-------------ISTMLLAVSCCFIITTAPIVIIKSISSVINKSLIDIFKYVKFELLESFAELLMYLNHGINFYLYLLTGSKFRKDIKTLFTNHRT 418
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
dmsr-72.809e-725.77DroMyoSuppressin Receptor related [Source:UniProt... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2T7NJU46.568e-4636.36G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A0K0PUW35.692e-4535.99Orphan G-protein coupled receptor 57 OS=Platynerei... [more]
A0A2H1CXP97.949e-4435.267 transmembrane receptor OS=Fasciola hepatica OX=6... [more]
A0A504YW103.231e-4334.49G-protein coupled receptor OS=Fasciola gigantica O... [more]
A0A183A9A84.349e-4333.07G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSAMXT00000035261.11.209e-1026.69probable G-protein coupled receptor 139 [Source:NC... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSORLT00000042752.19.934e-826.58pep primary_assembly:ASM223467v1:24:10229220:10232... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000075029.16.546e-4398.29SMESG000075029.1[more]
SMESG000020718.12.127e-5536.86SMESG000020718.1[more]
SMESG000042142.14.698e-5033.62SMESG000042142.1[more]
SMESG000053893.13.882e-4936.56SMESG000053893.1[more]
SMESG000074092.15.072e-4733.14SMESG000074092.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015371 ID=SMED30015371|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1438bp
TTTCAATGCCCAAAGTTTGCGATCATGTCGATTTGGGATCGGTGAGCATT
TTAAGGAGTTTCCTAAGCAATATTTCTACTGAGAATCAGACAATGAATAT
CTTGAATTTGCAGAAAACTAGAGTTTTTATCAACGATACTGTAAATTGGG
AATCTTTACTTAATAACTACCGGCTACAATTGAGTAAAGCAATTGAAAAG
GCGAGCCCGGGCTCAGAATTAATGACATGCAACGAACAATTGGGAATTGT
ACGTCTTTTATGATATTAACAAAAAGACGAGTATCCTCCTATGTGTATCT
CAGCGCGTTGGCAATGGCAGATCAAGGCGTTTTAATAACAGGGTTAATGC
GGCATTGCATTGACAGAATTCTTGATTACAGATTAGAAGACTCACATCAC
TGGACCTGTGTGTTAACTCAACTGATCGGATCATCTTTATCATATCTTTC
AGCATGGCTGATCGTAGCCGTGACAGCAGAAAGGGCTATTGTAATTATGT
TTCCATTTTCATCTATAAGGTTGTACTCTCGATTTCGTGAAACTCTAGTT
GTTTTGTTGATTTCACTGGCTTGTTTATTAGCCTCTTCGCATTTTCTTCT
TTCGGTTCGTTTAGAAACCGGTCAAATGTATAGATTCAATTTAAACAACG
GATCGGTTCAGAACTATTCCCAGTGTCAACTGCGAAATGAGCTTCACAGT
TTTTCAAAGGCTTGGTATTGGTTAGACGGTTTGTTATATAGCTACCTTCC
GTTTTTTCTGATTTTATGTTTAAATGGTTTGATTGTGTCTCATGTGAGAA
AAGCTAGAGAACACAGAAATATTCTCGTATCTCGGTGCCCACAATCGAAT
TATGCACAAATAAAAGATTGTTCTGTAAGAAATTTGTCACATAGCGTCAA
TACTCAGGCAGGATCAAGACAAACGTTTCAATTAACATTGATGCTCTTTT
CCATATCTGCTTGTTTCCTCATTACCACTTGCCCAATTGTTGTTATGAAA
ATTGTCAATTTCACTCAAAATCGAGATACTCTAGATCAGAGAGGATCTGC
TCTTCACCAATTGTTGGACAGCATTGCAGAATTGTTGATGTATCTCAACC
ATTCAGCCAATTTCTATTTGTATTGTGCTACAGGCTCCAAATTCAGAAAT
GAACTGAAGTCTCTTTGCTCAGATACCCGAGCCACTCGAAGCAGACTGCA
AAACCAAACCACACTTGCCAAGTCACGAGTTCATCAGCCATATCCAGTTG
AGAAAGACCTATCAAACAGTTAATTTCTAAAAAGGGATAATCTGTAAAAA
ATCTAATTTATTCATTTCACAAATTCCTACCTGAAAGTCTACCAAAATTA
ATTAGCTCATAACCCGCACCAACATCTGGCGGACGCATGAAAATTTCGAC
ATAGACGCTTACGATTTATTGGTGACTGCTAAAATTAC
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protein sequence of SMED30015371-orf-1

>SMED30015371-orf-1 ID=SMED30015371-orf-1|Name=SMED30015371-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=349bp
MQRTIGNCTSFMILTKRRVSSYVYLSALAMADQGVLITGLMRHCIDRILD
YRLEDSHHWTCVLTQLIGSSLSYLSAWLIVAVTAERAIVIMFPFSSIRLY
SRFRETLVVLLISLACLLASSHFLLSVRLETGQMYRFNLNNGSVQNYSQC
QLRNELHSFSKAWYWLDGLLYSYLPFFLILCLNGLIVSHVRKAREHRNIL
VSRCPQSNYAQIKDCSVRNLSHSVNTQAGSRQTFQLTLMLFSISACFLIT
TCPIVVMKIVNFTQNRDTLDQRGSALHQLLDSIAELLMYLNHSANFYLYC
ATGSKFRNELKSLCSDTRATRSRLQNQTTLAKSRVHQPYPVEKDLSNS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0007165signal transduction
GO:0007186G-protein coupled receptor signaling pathway
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 4..324
e-value: 2.0E-42
score: 147.3
NoneNo IPR availablePANTHERPTHR46641:SF12CNMAMIDE RECEPTORcoord: 5..330
NoneNo IPR availablePANTHERPTHR46641FAMILY NOT NAMEDcoord: 5..330
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 4..310
e-value: 6.87302E-41
score: 143.157
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 5..323
NoneNo IPR availableTMHMMTMhelixcoord: 238..260
NoneNo IPR availableTMHMMTMhelixcoord: 168..190
NoneNo IPR availableTMHMMTMhelixcoord: 62..84
NoneNo IPR availableTMHMMTMhelixcoord: 20..42
NoneNo IPR availableTMHMMTMhelixcoord: 107..129
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 14..295
e-value: 8.7E-12
score: 44.9
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 1..299
score: 18.646