SMED30015361
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30015361 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Alignments
SMED30015361 aligns in the following genomic locations:
Homology
BLAST of SMED30015361 vs. Planmine SMEST
Match: SMESG000032256.1 (SMESG000032256.1) HSP 1 Score: 393.275 bits (1009), Expect = 2.789e-140 Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 3 Query: 222 MLSPIQMTIKTKSSFYKFDNLKPCRKYCLKIRGIDKLGIIAESKVICQYLKLTNFDAVTLNSVQSKKLPFILQWNYPNTSNCSDITFTVEYHLVKTSKPVYSLETNQTKVVLGNIEYDKSYLAKVRPEHDKDYGSFSKELEIFIKKTPDIPIFTPKPDISFTTQISNNATVLKSMRFVLFGRMRVVNVKTKS 797 MLSPIQMTIKTKSSFYKFDNLKPCRKYCLKIRGIDKLGIIAESKVICQYLKLTNFDAVTLNSVQSKKLPFILQWNYPNTSNCSDITFTVEYHLVKTSKPVYSLETNQTKVVLGNIEYDKSYLAKVRPEHDKDYGSFSKELEIFIKKTPDIPIFTPKPDISFTTQISNNATVLKSMRFVLFGRMRVVNVKTKS Sbjct: 1 MLSPIQMTIKTKSSFYKFDNLKPCRKYCLKIRGIDKLGIIAESKVICQYLKLTNFDAVTLNSVQSKKLPFILQWNYPNTSNCSDITFTVEYHLVKTSKPVYSLETNQTKVVLGNIEYDKSYLAKVRPEHDKDYGSFSKELEIFIKKTPDIPIFTPKPDISFTTQISNNATVLKSMRFVLFGRMRVVNVKTKS 192
BLAST of SMED30015361 vs. Planmine SMEST
Match: SMESG000032256.1 (SMESG000032256.1) HSP 1 Score: 380.563 bits (976), Expect = 2.119e-135 Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 3 Query: 240 MTIKTKSSFYKFDNLKPCRKYCLKIRGIDKLGIIAESKVICQYLKLTNFDAVTLNSVQSKKLPFILQWNYPNTSNCSDITFTVEYHLVKTSKPVYSLETNQTKVVLGNIEYDKSYLAKVRPEHDKDYGSFSKELEIFIKKTPDIPIFTPKPDISFTTQISNNATVLKSMRFVLFGRMRVVNVKTKS 797 MTIKTKSSFYKFDNLKPCRKYCLKIRGIDKLGIIAESKVICQYLKLTNFDAVTLNSVQSKKLPFILQWNYPNTSNCSDITFTVEYHLVKTSKPVYSLETNQTKVVLGNIEYDKSYLAKVRPEHDKDYGSFSKELEIFIKKTPDIPIFTPKPDISFTTQISNNATVLKSMRFVLFGRMRVVNVKTKS Sbjct: 1 MTIKTKSSFYKFDNLKPCRKYCLKIRGIDKLGIIAESKVICQYLKLTNFDAVTLNSVQSKKLPFILQWNYPNTSNCSDITFTVEYHLVKTSKPVYSLETNQTKVVLGNIEYDKSYLAKVRPEHDKDYGSFSKELEIFIKKTPDIPIFTPKPDISFTTQISNNATVLKSMRFVLFGRMRVVNVKTKS 186
BLAST of SMED30015361 vs. Planmine SMEST
Match: SMESG000032167.1 (SMESG000032167.1) HSP 1 Score: 142.124 bits (357), Expect = 9.908e-38 Identity = 79/185 (42.70%), Postives = 108/185 (58.38%), Query Frame = 3 Query: 135 EIKVYDKTSKSFWILYKHKTLKSI--EYTLIMLSPIQMTIKTKSSFYKFDNLKPCRKYCLKIRGIDKLGIIAESKVICQYLKLTNFDAVTLNSVQSKKLPFILQWNYPNTSNCSDITFTVEYHLVKTSKPVYSLETNQTKVVLGNIEYDKSYLAKVRPEHDKDYGSFSKELEIFIKKTPDIPIFT 683 EI V++ TS SF I +K +Y +I+L P ++ KTK + + DNL PC +YC +I + +SK ICQ +T FD V L ++S +LP +L W Y NT NCS I F +EY+L K +YS+ TN T V L I+Y Y AKV+PE D +G+FS+ELEI I K P+ P T Sbjct: 544 EIVVFNVTSTSFQIRWKMPPEVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNITLFDEVVLKPIESTQLPAVLYWTYQNTLNCSHIIFYIEYYLENNKKNIYSISTNDTMVKLEQIKYKTKYFAKVKPEFDGLFGNFSEELEIVINKMPETPSIT 728 HSP 2 Score: 139.043 bits (349), Expect = 1.126e-36 Identity = 70/150 (46.67%), Postives = 95/150 (63.33%), Query Frame = 3 Query: 210 YTLIMLSPIQMTIKTKSSFYKFDNLKPCRKYCLKIRGIDKLGIIAESKVICQYLKLTNFDAVTLNSVQSKKLPFILQWNYPNTSNCSDITFTVEYHLVKTSKPVYSLETNQTKVVLGNIEYDKSYLAKVRPEHDKDYGSFSKELEIFIKK 659 Y ++ML P T +TK + + +NL PCRKYC IR + +GI S IC+ LT FD V L +Q P +L W Y NTSNCS I F VEY+ K K +YS+ TN+T+ L IE++K Y KVRP++++ +G+FSK LE+FIKK Sbjct: 753 YEIVMLFPKTSTYRTKQTNFTLENLLPCRKYCFGIRSFNLVGISNLSMEICEKTNLTAFDEVVLKPLQDNSFPVVLSWTYINTSNCSGILFIVEYYSDKEDKDIYSITTNKTESELEKIEFNKKYAVKVRPKYEEMFGNFSKILELFIKK 902 HSP 3 Score: 128.642 bits (322), Expect = 4.283e-33 Identity = 71/169 (42.01%), Postives = 95/169 (56.21%), Query Frame = 3 Query: 207 EYTLIMLSPIQMTIKTKSSFYKFDNLKPCRKYCLKIRGIDKLGIIAESKVICQ-------------YLKLTNFDAVTLNSVQSKKLPFILQWNYPNTSNCSDITFTVEYHLVKTSKPVYSLETNQTKVVLGNIEYDKSYLAKVRPEHDKDYGSFSKELEIFIKKTPDIP 674 EY +I+ P ++ KTK + + DNL PC +YC +I + G +SK I Q Y L FD V L ++S +LP +L W Y NT NCS+I F +EY+L K +YS+ TN T V L I+Y Y AKV+PE D +G+FS+ELEI I K PD P Sbjct: 10 EYEIILRHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFGESKKSKKIYQKTNISIKTIQNIIYDNLVLFDEVVLKPIESTQLPAVLYWTYQNTLNCSNIIFYIEYYLENNKKNIYSISTNDTMVKLEQIKYKTKYFAKVKPEFDGLFGNFSEELEIVINKMPDPP 178 HSP 4 Score: 119.398 bits (298), Expect = 5.239e-30 Identity = 66/155 (42.58%), Postives = 96/155 (61.94%), Query Frame = 3 Query: 210 YTLIMLSPIQMTIKTKSSFYKFDNLKPCRKYCLKIRGIDKLGIIAESKVICQYLKLTNFDAVTLNSVQSKKLPFILQWNYPNTSNCSDITFTVEYHLVKTSKPVYSLETNQTKVVLGNIEYDKSYLAKVRPEHDKDYGSFSKELEIFIKKTPDIP 674 Y ++++ ++T +T + +K DNL CR+YC I+ +KLG SK ICQ K+ +FD VTLN V S+KLP L+ +Y NT NCS+I F V+Y+ S PVY + TN+T + +EY+ Y K R E++ + ++SKELEI I K P P Sbjct: 207 YEIVVIHSEKITYQTYKTKFKLDNLLTCRQYCFAIQVFNKLGKSELSKEICQNTKIASFDEVTLNPVNSQKLPVTLEVSYLNTLNCSNILFIVKYYSEIDSHPVYFI-TNETATEIQEMEYNIKYYVKARCEYEGMFSNYSKELEILITKVPITP 360 HSP 5 Score: 109.383 bits (272), Expect = 1.422e-26 Identity = 59/153 (38.56%), Postives = 84/153 (54.90%), Query Frame = 3 Query: 219 IMLSPIQMTIKTKSSFYKFDNLKPCRKYCLKIRGIDKLGIIAESKVICQYLKLTNFDAVTLNSVQSKKLPFILQWNYPNTSNCSDITFTVEYHLVKTSKPVYSLETNQTKVVLGNIEYDKSYLAKVRPEHDKDYGSFSKELEIFIKKTPDIPI 677 +++ PI +TK + F NL PCR YC ++R + S+ CQ K+ FD V L+ V P IL+W Y NT CSDI F V Y+ +Y+ TN+ ++ L IE+D Y AKV+PE+D +G+FSKE+ I + P PI Sbjct: 391 VLVIPIGAIYRTKETDLTFVNLLPCRTYCFQVRIFNFWTSSELSEKKCQSTKIAYFDEVILSPVVYNGFPVILEWTYQNTLGCSDIFFNVVYYPTYRENIIYTNRTNRKELQLDKIEHDLIYFAKVKPEYDGMFGNFSKEMLILVTSKPVKPI 543
BLAST of SMED30015361 vs. Planmine SMEST
Match: SMESG000032101.1 (SMESG000032101.1) HSP 1 Score: 139.428 bits (350), Expect = 3.547e-37 Identity = 77/185 (41.62%), Postives = 108/185 (58.38%), Query Frame = 3 Query: 135 EIKVYDKTSKSFWILYKHKTLKSI--EYTLIMLSPIQMTIKTKSSFYKFDNLKPCRKYCLKIRGIDKLGIIAESKVICQYLKLTNFDAVTLNSVQSKKLPFILQWNYPNTSNCSDITFTVEYHLVKTSKPVYSLETNQTKVVLGNIEYDKSYLAKVRPEHDKDYGSFSKELEIFIKKTPDIPIFT 683 EI V++ TS SF IL+K +Y +I+L P ++ KTK + + DNL PC +YC +I + +SK ICQ +T FD L ++S +LP +L W Y NT NCS+I F +EY+L K +YS+ TN T V L I+Y Y A+V+P D +G+FS+ELEI I K P+ P T Sbjct: 194 EIVVFNVTSTSFQILWKMPPEVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNITLFDEAVLKPIESTQLPAVLYWTYQNTLNCSNIIFYIEYYLENNKKNIYSISTNDTMVKLEQIKYKTKYFARVKPIFDGLFGNFSEELEIVINKMPETPSIT 378 HSP 2 Score: 119.013 bits (297), Expect = 6.456e-30 Identity = 60/135 (44.44%), Postives = 81/135 (60.00%), Query Frame = 3 Query: 210 YTLIMLSPIQMTIKTKSSFYKFDNLKPCRKYCLKIRGIDKLGIIAESKVICQYLKLTNFDAVTLNSVQSKKLPFILQWNYPNTSNCSDITFTVEYHLVKTSKPVYSLETNQTKVVLGNIEYDKSYLAKVRPEHDK 614 Y ++ML P T +TK + + NL PCRKYC IR + +GI S IC+ LT FD V L +Q P +L W Y NTSNCS I F VEY+ K K +YS+ TN+T+ L IE++K Y KVRP++++ Sbjct: 403 YEIVMLFPKTSTYRTKQTNFTLQNLLPCRKYCFGIRSFNLVGISNLSMEICEKTNLTAFDEVVLKPLQDSSFPVVLSWTYINTSNCSGILFIVEYYSDKEDKDIYSIITNKTESELEKIEFNKKYAVKVRPKYEE 537 HSP 3 Score: 43.8986 bits (102), Expect = 1.883e-7 Identity = 23/55 (41.82%), Postives = 33/55 (60.00%), Query Frame = 3 Query: 510 SKPVYSLETNQTKVVLGNIEYDKSYLAKVRPEHDKDYGSFSKELEIFIKKTPDIP 674 S PVY + TN+T + +EY+ Y K R E++ + ++SKELEI I K P P Sbjct: 51 SHPVYFI-TNETATEIQEMEYNIKYYVKARCEYEGMFSNYSKELEILITKVPITP 104 HSP 4 Score: 29.6462 bits (65), Expect = 1.883e-7 Identity = 14/33 (42.42%), Postives = 21/33 (63.64%), Query Frame = 2 Query: 392 SDIEFCSVQKATIYFTMELSEYFKLF*YHIYRR 490 SD+E C KA+ + LSEYF+LF Y ++ + Sbjct: 12 SDLEPCQFTKASSHLGGVLSEYFELFKYSLHSK 44
BLAST of SMED30015361 vs. Planmine SMEST
Match: SMESG000032101.1 (SMESG000032101.1) HSP 1 Score: 139.428 bits (350), Expect = 4.338e-37 Identity = 77/185 (41.62%), Postives = 108/185 (58.38%), Query Frame = 3 Query: 135 EIKVYDKTSKSFWILYKHKTLKSI--EYTLIMLSPIQMTIKTKSSFYKFDNLKPCRKYCLKIRGIDKLGIIAESKVICQYLKLTNFDAVTLNSVQSKKLPFILQWNYPNTSNCSDITFTVEYHLVKTSKPVYSLETNQTKVVLGNIEYDKSYLAKVRPEHDKDYGSFSKELEIFIKKTPDIPIFT 683 EI V++ TS SF IL+K +Y +I+L P ++ KTK + + DNL PC +YC +I + +SK ICQ +T FD L ++S +LP +L W Y NT NCS+I F +EY+L K +YS+ TN T V L I+Y Y A+V+P D +G+FS+ELEI I K P+ P T Sbjct: 247 EIVVFNVTSTSFQILWKMPPEVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNITLFDEAVLKPIESTQLPAVLYWTYQNTLNCSNIIFYIEYYLENNKKNIYSISTNDTMVKLEQIKYKTKYFARVKPIFDGLFGNFSEELEIVINKMPETPSIT 431 HSP 2 Score: 119.013 bits (297), Expect = 6.826e-30 Identity = 60/135 (44.44%), Postives = 81/135 (60.00%), Query Frame = 3 Query: 210 YTLIMLSPIQMTIKTKSSFYKFDNLKPCRKYCLKIRGIDKLGIIAESKVICQYLKLTNFDAVTLNSVQSKKLPFILQWNYPNTSNCSDITFTVEYHLVKTSKPVYSLETNQTKVVLGNIEYDKSYLAKVRPEHDK 614 Y ++ML P T +TK + + NL PCRKYC IR + +GI S IC+ LT FD V L +Q P +L W Y NTSNCS I F VEY+ K K +YS+ TN+T+ L IE++K Y KVRP++++ Sbjct: 456 YEIVMLFPKTSTYRTKQTNFTLQNLLPCRKYCFGIRSFNLVGISNLSMEICEKTNLTAFDEVVLKPLQDSSFPVVLSWTYINTSNCSGILFIVEYYSDKEDKDIYSIITNKTESELEKIEFNKKYAVKVRPKYEE 590 HSP 3 Score: 83.5741 bits (205), Expect = 7.895e-18 Identity = 44/112 (39.29%), Postives = 65/112 (58.04%), Query Frame = 3 Query: 219 IMLSPIQMTIKTKSSFYKFDNLKPCRKYCLKIRGIDKLGIIAESKVICQYLKLTNFDAVTLNSVQSKKLPFILQWNYPNTSNCSDITFTVEYHLVKTSKPVYSLETNQTKVV 554 +++ PI +TK + F NL PCR YC ++R + S+ CQ K+ +F VTL+S+Q+ LPFILQW Y NT NCS I+F V Y+ K + ++S+ T K + Sbjct: 135 VLVIPIGAIYRTKETDLTFVNLLPCRTYCFQVRIFNFWTSSELSEKKCQSTKIGSFGQVTLSSIQTMTLPFILQWYYENTFNCSGISFLVHYYSEKEIENIFSVTTKPVKPI 246 HSP 4 Score: 43.8986 bits (102), Expect = 1.690e-7 Identity = 23/55 (41.82%), Postives = 33/55 (60.00%), Query Frame = 3 Query: 510 SKPVYSLETNQTKVVLGNIEYDKSYLAKVRPEHDKDYGSFSKELEIFIKKTPDIP 674 S PVY + TN+T + +EY+ Y K R E++ + ++SKELEI I K P P Sbjct: 51 SHPVYFI-TNETATEIQEMEYNIKYYVKARCEYEGMFSNYSKELEILITKVPITP 104 HSP 5 Score: 29.6462 bits (65), Expect = 1.690e-7 Identity = 14/33 (42.42%), Postives = 21/33 (63.64%), Query Frame = 2 Query: 392 SDIEFCSVQKATIYFTMELSEYFKLF*YHIYRR 490 SD+E C KA+ + LSEYF+LF Y ++ + Sbjct: 12 SDLEPCQFTKASSHLGGVLSEYFELFKYSLHSK 44 The following BLAST results are available for this feature:
BLAST of SMED30015361 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30015361 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30015361 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30015361 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30015361 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30015361 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30015361 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30015361 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30015361 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30015361 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30015361 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30015361 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30015361 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30015361 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30015361 ID=SMED30015361|Name=SMED30015361|organism=Schmidtea mediterranea sexual|type=transcript|length=860bpback to top protein sequence of SMED30015361-orf-1 >SMED30015361-orf-1 ID=SMED30015361-orf-1|Name=SMED30015361-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=266bp VMSKYTGLKFLIYCIIFNFSLIQCSDKKIKTQTNNQLSNKESEFEIKVYDback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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