translocon associated protein subunit delta

Overview
Nametranslocon associated protein subunit delta (AFJ24839.1)
Smed IDSMED30015265
Length (bp)647
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of translocon associated protein subunit delta (SMED30015265) t-SNE clustered cells

Violin plots show distribution of expression levels for translocon associated protein subunit delta (SMED30015265) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of translocon associated protein subunit delta (SMED30015265) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for translocon associated protein subunit delta (SMED30015265) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30015265

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30015265h1SMcG0007357 dd_Smed_v4_2051_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30015265h1SMcG0007357 dd_Smed_v4_2051_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30015265h1SMcG0007357 dd_Smed_v4_2051_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30015265h1SMcG0007357 dd_Smed_v4_2051_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30015265h1SMcG0007357 dd_Smed_v4_2051_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30015265h1SMcG0007357 dd_Smed_v4_2051_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of translocon associated protein subunit delta vs. Ensembl Human
Match: SSR4 (signal sequence receptor subunit 4 [Source:HGNC Symbol;Acc:HGNC:11326])

HSP 1 Score: 117.472 bits (293), Expect = 1.230e-32
Identity = 53/149 (35.57%), Postives = 91/149 (61.07%), Query Frame = 3
Query:   93 TCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
             C  PQ+  + Y+T +A++S+ TVF+ +I+L C  +   + LYA++ G    V +  +  +YQV++   H+   A  Y ++F+DE++YS++RK QR+ E IS I PLFT++  H+G   GP VSTE +   +  ++ +  FS ++ I A
Sbjct:   25 ACLEPQITPSYYTTSDAVISTETVFIVEISLTCKNRVQNMALYADVGGKQFPVTRGQDVGRYQVSWSLDHKSAHAGTYEVRFFDEESYSLLRKAQRNNEDISIIPPLFTVSVDHRGTWNGPWVSTEVLAAAIGLVIYYLAFSAKSHIQA 173          
BLAST of translocon associated protein subunit delta vs. Ensembl Human
Match: SSR4 (signal sequence receptor subunit 4 [Source:HGNC Symbol;Acc:HGNC:11326])

HSP 1 Score: 117.472 bits (293), Expect = 1.230e-32
Identity = 53/149 (35.57%), Postives = 91/149 (61.07%), Query Frame = 3
Query:   93 TCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
             C  PQ+  + Y+T +A++S+ TVF+ +I+L C  +   + LYA++ G    V +  +  +YQV++   H+   A  Y ++F+DE++YS++RK QR+ E IS I PLFT++  H+G   GP VSTE +   +  ++ +  FS ++ I A
Sbjct:   25 ACLEPQITPSYYTTSDAVISTETVFIVEISLTCKNRVQNMALYADVGGKQFPVTRGQDVGRYQVSWSLDHKSAHAGTYEVRFFDEESYSLLRKAQRNNEDISIIPPLFTVSVDHRGTWNGPWVSTEVLAAAIGLVIYYLAFSAKSHIQA 173          
BLAST of translocon associated protein subunit delta vs. Ensembl Human
Match: SSR4 (signal sequence receptor subunit 4 [Source:HGNC Symbol;Acc:HGNC:11326])

HSP 1 Score: 117.472 bits (293), Expect = 1.230e-32
Identity = 53/149 (35.57%), Postives = 91/149 (61.07%), Query Frame = 3
Query:   93 TCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
             C  PQ+  + Y+T +A++S+ TVF+ +I+L C  +   + LYA++ G    V +  +  +YQV++   H+   A  Y ++F+DE++YS++RK QR+ E IS I PLFT++  H+G   GP VSTE +   +  ++ +  FS ++ I A
Sbjct:   25 ACLEPQITPSYYTTSDAVISTETVFIVEISLTCKNRVQNMALYADVGGKQFPVTRGQDVGRYQVSWSLDHKSAHAGTYEVRFFDEESYSLLRKAQRNNEDISIIPPLFTVSVDHRGTWNGPWVSTEVLAAAIGLVIYYLAFSAKSHIQA 173          
BLAST of translocon associated protein subunit delta vs. Ensembl Human
Match: SSR4 (signal sequence receptor subunit 4 [Source:HGNC Symbol;Acc:HGNC:11326])

HSP 1 Score: 93.5893 bits (231), Expect = 9.943e-24
Identity = 47/148 (31.76%), Postives = 80/148 (54.05%), Query Frame = 3
Query:   96 CNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
            C  PQ+  + Y+T +A++S+ TVF+ +I+L C                          ++ QV++   H+   A  Y ++F+DE++YS++RK QR+ E IS I PLFT++  H+G   GP VSTE +   +  ++ +  FS ++ I A
Sbjct:   26 CLEPQITPSYYTTSDAVISTETVFIVEISLTCK-------------------------NRVQVSWSLDHKSAHAGTYEVRFFDEESYSLLRKAQRNNEDISIIPPLFTVSVDHRGTWNGPWVSTEVLAAAIGLVIYYLAFSAKSHIQA 148          
BLAST of translocon associated protein subunit delta vs. Ensembl Celegans
Match: trap-4 (TRanslocon-Associated Protein [Source:UniProtKB/TrEMBL;Acc:Q9U238])

HSP 1 Score: 75.8702 bits (185), Expect = 2.921e-17
Identity = 38/149 (25.50%), Postives = 73/149 (48.99%), Query Frame = 3
Query:   42 IFNVLLIFSVIYSVTATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILL 488
            +  + +   ++ S     C +P+   +++ST +     +T F+T+ TL+C      I   AE+NG +  V    ET K+QV++   H+   A  + +  +DE+  +   KN  +       KPLFT+   H G+     +S+ET+ +++
Sbjct:    1 MLKIAVALCLVASALCAKCESPKYSASSFSTTDGFFHYKTTFITEFTLQCSNNPKNIQYTAEVNGRLIPVSISDETAKFQVSWTLEHKDAGAQTFNINIFDEEGAAQYAKNPVT-------KPLFTVQQSHGGLATKSPISSETVAVII 142          
BLAST of translocon associated protein subunit delta vs. Ensembl Celegans
Match: trap-4 (TRanslocon-Associated Protein [Source:UniProtKB/TrEMBL;Acc:Q9U238])

HSP 1 Score: 75.8702 bits (185), Expect = 2.921e-17
Identity = 38/149 (25.50%), Postives = 73/149 (48.99%), Query Frame = 3
Query:   42 IFNVLLIFSVIYSVTATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILL 488
            +  + +   ++ S     C +P+   +++ST +     +T F+T+ TL+C      I   AE+NG +  V    ET K+QV++   H+   A  + +  +DE+  +   KN  +       KPLFT+   H G+     +S+ET+ +++
Sbjct:    1 MLKIAVALCLVASALCAKCESPKYSASSFSTTDGFFHYKTTFITEFTLQCSNNPKNIQYTAEVNGRLIPVSISDETAKFQVSWTLEHKDAGAQTFNINIFDEEGAAQYAKNPVT-------KPLFTVQQSHGGLATKSPISSETVAVII 142          
BLAST of translocon associated protein subunit delta vs. Ensembl Fly
Match: Tapdelta (gene:FBgn0021795 transcript:FBtr0345952)

HSP 1 Score: 80.4925 bits (197), Expect = 8.924e-19
Identity = 46/167 (27.54%), Postives = 89/167 (53.29%), Query Frame = 3
Query:   36 KMIFNVLLIFSVIYSVTATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELN-GHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKI 533
            + +F + LI +V  S    +C   +   +++ST++A + ++   V + TL+C G A   +L+AE   G +  V K  + +KYQV++ +  ++  + N  ++ +DED Y+ +RK QR G+ +S +K L  I+   +G   GP +  E +   L     +  F+ + K+
Sbjct:    3 RQLFAICLIAAVCASGVYGSC---KATVSSFSTQDATILTQLAHVGEFTLQCSG-AAPASLFAEFPCGKVVPVAKVGD-NKYQVSWVEDIKQGSSGNVQVRLFDEDGYANVRKAQRDGDKVSSVKSLLDISVPTKGAYKGPWIKAELLAAFLVGGFAYFAFTTKGKV 164          
BLAST of translocon associated protein subunit delta vs. Ensembl Fly
Match: Tapdelta (gene:FBgn0021795 transcript:FBtr0088153)

HSP 1 Score: 80.4925 bits (197), Expect = 8.924e-19
Identity = 46/167 (27.54%), Postives = 89/167 (53.29%), Query Frame = 3
Query:   36 KMIFNVLLIFSVIYSVTATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELN-GHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKI 533
            + +F + LI +V  S    +C   +   +++ST++A + ++   V + TL+C G A   +L+AE   G +  V K  + +KYQV++ +  ++  + N  ++ +DED Y+ +RK QR G+ +S +K L  I+   +G   GP +  E +   L     +  F+ + K+
Sbjct:    3 RQLFAICLIAAVCASGVYGSC---KATVSSFSTQDATILTQLAHVGEFTLQCSG-AAPASLFAEFPCGKVVPVAKVGD-NKYQVSWVEDIKQGSSGNVQVRLFDEDGYANVRKAQRDGDKVSSVKSLLDISVPTKGAYKGPWIKAELLAAFLVGGFAYFAFTTKGKV 164          
BLAST of translocon associated protein subunit delta vs. Ensembl Zebrafish
Match: ssr4 (signal sequence receptor, delta [Source:ZFIN;Acc:ZDB-GENE-030131-4900])

HSP 1 Score: 133.265 bits (334), Expect = 4.434e-39
Identity = 61/169 (36.09%), Postives = 104/169 (61.54%), Query Frame = 3
Query:   33 VKMIFNVLLIFSVIYSVTATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
            +K++   LL   + +   A TC +P +  ++Y+T +AL+SS TVF+ +++L C   A  + LYA++NG    V +  +  KYQV++   H++  +  Y +KF+DE++YS +RK QR+ E +  IKPLF++N  H+G   GP VSTE +  L+  +V +  +S ++ I A
Sbjct:    1 MKLVLTTLLALCLSF-CAAETCTDPVISPSSYTTTDALISSETVFIVELSLACANGAQSVALYADVNGKQFPVTRGQDVGKYQVSWSVPHKQASSGTYQVKFFDEESYSTLRKAQRNNEDVDAIKPLFSVNVDHRGAWNGPWVSTEVLAALIGIMVYYLAYSTKSAIQA 168          
BLAST of translocon associated protein subunit delta vs. Ensembl Zebrafish
Match: ssr4 (signal sequence receptor, delta [Source:ZFIN;Acc:ZDB-GENE-030131-4900])

HSP 1 Score: 129.413 bits (324), Expect = 1.100e-37
Identity = 57/149 (38.26%), Postives = 95/149 (63.76%), Query Frame = 3
Query:   93 TCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
            TC +P +  ++Y+T +AL+SS TVF+ +++L C   A  + LYA++NG    V +  +  KYQV++   H++  +  Y +KF+DE++YS +RK QR+ E +  IKPLF++N  H+G   GP VSTE +  L+  +V +  +S ++ I A
Sbjct:   11 TCTDPVISPSSYTTTDALISSETVFIVELSLACANGAQSVALYADVNGKQFPVTRGQDVGKYQVSWSVPHKQASSGTYQVKFFDEESYSTLRKAQRNNEDVDAIKPLFSVNVDHRGAWNGPWVSTEVLAALIGIMVYYLAYSTKSAIQA 159          
BLAST of translocon associated protein subunit delta vs. Ensembl Xenopus
Match: ftsj1 (FtsJ RNA methyltransferase homolog 1 [Source:Xenbase;Acc:XB-GENE-5798521])

HSP 1 Score: 124.405 bits (311), Expect = 1.439e-35
Identity = 57/154 (37.01%), Postives = 96/154 (62.34%), Query Frame = 3
Query:   78 SVTATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
            S+ + TC  P +  + Y+T +A++SS  VF+ +I+L C   A  + LYA++NG    V +  +  +YQV++   H+   +  Y +KF+DE++YS++RK QR+ E IS I+PLFT+N  H+G   GP VSTE +  L+  +V +  ++ ++ I A
Sbjct:   12 SLGSETCTEPVIVPSYYTTSDAVISSEVVFIVEISLSCTNGAQNVALYADVNGKQFPVTRGQDVGRYQVSWSLEHKNARSGTYEVKFFDEESYSLLRKAQRNNEDISSIQPLFTVNVDHRGAWNGPWVSTEVLAGLIGILVYYTAYTAKSSIQA 165          
BLAST of translocon associated protein subunit delta vs. Ensembl Xenopus
Match: ftsj1 (FtsJ RNA methyltransferase homolog 1 [Source:Xenbase;Acc:XB-GENE-5798521])

HSP 1 Score: 124.02 bits (310), Expect = 2.471e-35
Identity = 56/153 (36.60%), Postives = 95/153 (62.09%), Query Frame = 3
Query:   81 VTATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
            ++  TC  P +  + Y+T +A++SS  VF+ +I+L C   A  + LYA++NG    V +  +  +YQV++   H+   +  Y +KF+DE++YS++RK QR+ E IS I+PLFT+N  H+G   GP VSTE +  L+  +V +  ++ ++ I A
Sbjct:   10 ISPETCTEPVIVPSYYTTSDAVISSEVVFIVEISLSCTNGAQNVALYADVNGKQFPVTRGQDVGRYQVSWSLEHKNARSGTYEVKFFDEESYSLLRKAQRNNEDISSIQPLFTVNVDHRGAWNGPWVSTEVLAGLIGILVYYTAYTAKSSIQA 162          
BLAST of translocon associated protein subunit delta vs. Ensembl Xenopus
Match: ftsj1 (FtsJ RNA methyltransferase homolog 1 [Source:Xenbase;Acc:XB-GENE-5798521])

HSP 1 Score: 124.02 bits (310), Expect = 2.471e-35
Identity = 56/153 (36.60%), Postives = 95/153 (62.09%), Query Frame = 3
Query:   81 VTATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
            ++  TC  P +  + Y+T +A++SS  VF+ +I+L C   A  + LYA++NG    V +  +  +YQV++   H+   +  Y +KF+DE++YS++RK QR+ E IS I+PLFT+N  H+G   GP VSTE +  L+  +V +  ++ ++ I A
Sbjct:   10 ISPETCTEPVIVPSYYTTSDAVISSEVVFIVEISLSCTNGAQNVALYADVNGKQFPVTRGQDVGRYQVSWSLEHKNARSGTYEVKFFDEESYSLLRKAQRNNEDISSIQPLFTVNVDHRGAWNGPWVSTEVLAGLIGILVYYTAYTAKSSIQA 162          
BLAST of translocon associated protein subunit delta vs. Ensembl Xenopus
Match: ftsj1 (FtsJ RNA methyltransferase homolog 1 [Source:Xenbase;Acc:XB-GENE-5798521])

HSP 1 Score: 123.25 bits (308), Expect = 3.685e-35
Identity = 56/149 (37.58%), Postives = 93/149 (62.42%), Query Frame = 3
Query:   93 TCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
            TC  P +  + Y+T +A++SS  VF+ +I+L C   A  + LYA++NG    V +  +  +YQV++   H+   +  Y +KF+DE++YS++RK QR+ E IS I+PLFT+N  H+G   GP VSTE +  L+  +V +  ++ ++ I A
Sbjct:    8 TCTEPVIVPSYYTTSDAVISSEVVFIVEISLSCTNGAQNVALYADVNGKQFPVTRGQDVGRYQVSWSLEHKNARSGTYEVKFFDEESYSLLRKAQRNNEDISSIQPLFTVNVDHRGAWNGPWVSTEVLAGLIGILVYYTAYTAKSSIQA 156          
BLAST of translocon associated protein subunit delta vs. Ensembl Xenopus
Match: ftsj1 (FtsJ RNA methyltransferase homolog 1 [Source:Xenbase;Acc:XB-GENE-5798521])

HSP 1 Score: 123.25 bits (308), Expect = 5.805e-35
Identity = 56/149 (37.58%), Postives = 93/149 (62.42%), Query Frame = 3
Query:   93 TCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
            TC  P +  + Y+T +A++SS  VF+ +I+L C   A  + LYA++NG    V +  +  +YQV++   H+   +  Y +KF+DE++YS++RK QR+ E IS I+PLFT+N  H+G   GP VSTE +  L+  +V +  ++ ++ I A
Sbjct:   22 TCTEPVIVPSYYTTSDAVISSEVVFIVEISLSCTNGAQNVALYADVNGKQFPVTRGQDVGRYQVSWSLEHKNARSGTYEVKFFDEESYSLLRKAQRNNEDISSIQPLFTVNVDHRGAWNGPWVSTEVLAGLIGILVYYTAYTAKSSIQA 170          
BLAST of translocon associated protein subunit delta vs. Ensembl Mouse
Match: Ssr4 (signal sequence receptor, delta [Source:MGI Symbol;Acc:MGI:1099464])

HSP 1 Score: 120.553 bits (301), Expect = 5.903e-34
Identity = 53/152 (34.87%), Postives = 95/152 (62.50%), Query Frame = 3
Query:   84 TATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
            +A  C  PQ+  + Y+T +A++S+ TVF+ +I+L C  +   + LYA+++G    V +  +  +YQV++   H+   A  Y ++F+DE++YS++RK QR+ E +S I PLFT++  H+G   GP VSTE +  ++  ++ +  FS ++ I A
Sbjct:   22 SAEACLEPQITPSYYTTSDAVISTETVFIVEISLTCKNRVQNMALYADVSGKQFPVTRGQDVGRYQVSWSLEHKSAHAGTYEVRFFDEESYSLLRKAQRNNEDVSIIPPLFTVSVDHRGTWNGPWVSTEVLAAVIGIVIYYLAFSAKSHIQA 173          
BLAST of translocon associated protein subunit delta vs. Ensembl Mouse
Match: Ssr4 (signal sequence receptor, delta [Source:MGI Symbol;Acc:MGI:1099464])

HSP 1 Score: 118.627 bits (296), Expect = 2.459e-33
Identity = 52/149 (34.90%), Postives = 93/149 (62.42%), Query Frame = 3
Query:   93 TCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
             C  PQ+  + Y+T +A++S+ TVF+ +I+L C  +   + LYA+++G    V +  +  +YQV++   H+   A  Y ++F+DE++YS++RK QR+ E +S I PLFT++  H+G   GP VSTE +  ++  ++ +  FS ++ I A
Sbjct:   24 ACLEPQITPSYYTTSDAVISTETVFIVEISLTCKNRVQNMALYADVSGKQFPVTRGQDVGRYQVSWSLEHKSAHAGTYEVRFFDEESYSLLRKAQRNNEDVSIIPPLFTVSVDHRGTWNGPWVSTEVLAAVIGIVIYYLAFSAKSHIQA 172          
BLAST of translocon associated protein subunit delta vs. UniProt/SwissProt
Match: sp|Q07984|SSRD_RAT (Translocon-associated protein subunit delta OS=Rattus norvegicus OX=10116 GN=Ssr4 PE=2 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 7.798e-33
Identity = 53/152 (34.87%), Postives = 94/152 (61.84%), Query Frame = 3
Query:   84 TATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
            +A  C  PQ+  + Y+T +A++S+ TVF+ +I+L C  +   + LYA+++G    V +  +  +YQV++   H+   A  Y ++F+DE++YS++RK QR+ E +S I PLFT++  H+G   GP VSTE +   +  ++ +  FS ++ I A
Sbjct:   22 SAEACLEPQITPSYYTTSDAVISTETVFIVEISLTCKNRVQNMALYADVSGKQFPVTRGQDVGRYQVSWSLEHKSAHAGTYEVRFFDEESYSLLRKAQRNNEDVSIIPPLFTVSVDHRGTWNGPWVSTEVLAAAIGIVIYYLAFSAKSHIQA 173          
BLAST of translocon associated protein subunit delta vs. UniProt/SwissProt
Match: sp|Q62186|SSRD_MOUSE (Translocon-associated protein subunit delta OS=Mus musculus OX=10090 GN=Ssr4 PE=1 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 1.720e-32
Identity = 52/149 (34.90%), Postives = 93/149 (62.42%), Query Frame = 3
Query:   93 TCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
             C  PQ+  + Y+T +A++S+ TVF+ +I+L C  +   + LYA+++G    V +  +  +YQV++   H+   A  Y ++F+DE++YS++RK QR+ E +S I PLFT++  H+G   GP VSTE +  ++  ++ +  FS ++ I A
Sbjct:   24 ACLEPQITPSYYTTSDAVISTETVFIVEISLTCKNRVQNMALYADVSGKQFPVTRGQDVGRYQVSWSLEHKSAHAGTYEVRFFDEESYSLLRKAQRNNEDVSIIPPLFTVSVDHRGTWNGPWVSTEVLAAVIGIVIYYLAFSAKSHIQA 172          
BLAST of translocon associated protein subunit delta vs. UniProt/SwissProt
Match: sp|Q5REH6|SSRD_PONAB (Translocon-associated protein subunit delta OS=Pongo abelii OX=9601 GN=SSR4 PE=2 SV=2)

HSP 1 Score: 117.472 bits (293), Expect = 5.908e-32
Identity = 53/149 (35.57%), Postives = 91/149 (61.07%), Query Frame = 3
Query:   93 TCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
             C  PQ+  + Y+T +A++S+ TVF+ +I+L C  +   + LYA++ G    V +  +  +YQV++   H+   A  Y ++F+DE++YS++RK QR+ E IS I PLFT++  H+G   GP VSTE +   +  ++ +  FS ++ I A
Sbjct:   25 ACLEPQITPSYYTTSDAVISTETVFIVEISLTCKNRVQNMALYADVGGKQFPVTRGQDVGRYQVSWSLDHKSAHAGTYEVRFFDEESYSLLRKAQRNNEDISIIPPLFTVSVDHRGTWNGPWVSTEVLAAAIGLVIYYLAFSAKSHIQA 173          
BLAST of translocon associated protein subunit delta vs. UniProt/SwissProt
Match: sp|P51571|SSRD_HUMAN (Translocon-associated protein subunit delta OS=Homo sapiens OX=9606 GN=SSR4 PE=1 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 5.908e-32
Identity = 53/149 (35.57%), Postives = 91/149 (61.07%), Query Frame = 3
Query:   93 TCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
             C  PQ+  + Y+T +A++S+ TVF+ +I+L C  +   + LYA++ G    V +  +  +YQV++   H+   A  Y ++F+DE++YS++RK QR+ E IS I PLFT++  H+G   GP VSTE +   +  ++ +  FS ++ I A
Sbjct:   25 ACLEPQITPSYYTTSDAVISTETVFIVEISLTCKNRVQNMALYADVGGKQFPVTRGQDVGRYQVSWSLDHKSAHAGTYEVRFFDEESYSLLRKAQRNNEDISIIPPLFTVSVDHRGTWNGPWVSTEVLAAAIGLVIYYLAFSAKSHIQA 173          
BLAST of translocon associated protein subunit delta vs. UniProt/SwissProt
Match: sp|Q2TBX5|SSRD_BOVIN (Translocon-associated protein subunit delta OS=Bos taurus OX=9913 GN=SSR4 PE=2 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 6.000e-32
Identity = 52/149 (34.90%), Postives = 92/149 (61.74%), Query Frame = 3
Query:   93 TCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
             C  PQ+  + Y+T +A++S+ TVF+ +I+L C  +   + LYA+++G    V +  +  +YQV++   H+   A  Y ++F+DE++YS++RK QR+ E +S I PLFT++  H+G   GP VSTE +   +  ++ +  FS ++ I A
Sbjct:   24 ACVEPQITPSYYTTSDAVISTETVFIVEISLTCKNRVQNMALYADVSGKQFPVTRGQDVGRYQVSWSLDHKSAHAGTYEVRFFDEESYSLLRKAQRNNEDVSVIPPLFTVSVDHRGTWNGPWVSTEVLAAAIGLVIYYLAFSAKSHIQA 172          
BLAST of translocon associated protein subunit delta vs. TrEMBL
Match: I1ZII5 (Translocon associated protein subunit delta OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 342.428 bits (877), Expect = 3.817e-118
Identity = 166/167 (99.40%), Postives = 166/167 (99.40%), Query Frame = 3
Query:   39 MIFNVLLIFSVIYSVTATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
            MIFNVL IFSVIYSVTATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA
Sbjct:    1 MIFNVLFIFSVIYSVTATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 167          
BLAST of translocon associated protein subunit delta vs. TrEMBL
Match: V9XRP2 (TRAPD (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 214.157 bits (544), Expect = 2.346e-68
Identity = 103/104 (99.04%), Postives = 104/104 (100.00%), Query Frame = 3
Query:   39 MIFNVLLIFSVIYSVTATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDED 350
            MIFNVLLIFSVIYSVTATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFY+ED
Sbjct:    1 MIFNVLLIFSVIYSVTATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYNED 104          
BLAST of translocon associated protein subunit delta vs. TrEMBL
Match: A0A2C9M917 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106071967 PE=4 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 7.682e-42
Identity = 64/152 (42.11%), Postives = 100/152 (65.79%), Query Frame = 3
Query:   84 TATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
            +A TC  PQ++  TYST EA++S+ TV + + TL C      INLYA++ G +    K +E +KYQ++  D H+KLPA  Y ++ +DE+ ++++RK QRSGES   IKPLF +N +H G+  GPIV TE +  L + ++ +  ++ R+K+LA
Sbjct:   21 SADTCLAPQIKSQTYSTPEAIVSTETVLIAEFTLTCKNSLKNINLYADVGGRLIPATKTSEVNKYQISITDDHKKLPAGRYDIRVFDEEGFALLRKAQRSGESTDSIKPLFVLNLNHSGIWNGPIVQTEFVAALAAILIWWLAYTARSKLLA 172          
BLAST of translocon associated protein subunit delta vs. TrEMBL
Match: K1QFG9 (Translocon-associated protein subunit delta OS=Crassostrea gigas OX=29159 GN=CGI_10020307 PE=4 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 1.870e-39
Identity = 67/166 (40.36%), Postives = 108/166 (65.06%), Query Frame = 3
Query:   36 KMIFNVLLIFSVIYSVTATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKI 533
            KMI  V+L+  V + V   +C +P+V+  TY+T EA++S+ TVFV   TL C  +   +NLYA++NG    V K  + D YQV+  D H+KL +  Y ++ YDE+ YS++RK QRSGES+  +KP+ +I F+H GV  GP V +E + ++++ +V +  +S R+++
Sbjct:    6 KMISLVVLLI-VPFVVLGDSCLSPEVKAETYTTTEAIVSTETVFVVQFTLTCKNKVRDMNLYADINGRTLPVTKTDKPDLYQVSISDDHKKLSSGKYEVRLYDEEGYSMLRKAQRSGESVEAVKPVTSITFNHPGVSKGPFVQSEFVAVVIAVLVWYFAYSTRSQL 170          
BLAST of translocon associated protein subunit delta vs. TrEMBL
Match: A0A3S1CBR4 (Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_003863 PE=4 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 5.156e-39
Identity = 62/169 (36.69%), Postives = 106/169 (62.72%), Query Frame = 3
Query:   42 IFNVLLIFSVIY---SVTATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
            +   L+ F+++    S++  TC  P+V   +YST EA++S  TV + + +L C      INLYA++ G      K +E +KYQ++  D  +KLP+  Y ++F+DE+ ++++RK QRSGES  ++KPLFTIN +H G+  GP++ +E +  + + IV +  ++ R K+LA
Sbjct:    4 LLKTLVAFTLVLLPVSISGDTCLAPKVTSQSYSTPEAVVSFDTVIIVEFSLTCKNNLKNINLYADVAGRTLPATKTSEPNKYQISLTDEPKKLPSGAYEIRFFDEEGFALLRKAQRSGESTGNLKPLFTINLNHPGIWSGPLIQSEFVAAMTAIIVWWMAYTQRGKLLA 172          
BLAST of translocon associated protein subunit delta vs. Ensembl Cavefish
Match: ssr4 (translocon-associated protein subunit delta-like [Source:NCBI gene;Acc:111194122])

HSP 1 Score: 129.028 bits (323), Expect = 1.991e-37
Identity = 56/152 (36.84%), Postives = 97/152 (63.82%), Query Frame = 3
Query:   84 TATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
             A TC +P +  ++Y+T +A++SS TVF+ +++L C   A  + LYA+++G    V +  +  KYQV++   H +  +  Y +KF+DE++YS++RK QR+ E +  IKPLFT+N  H+G   GP VSTE +  ++  ++ +  +S++N I A
Sbjct:   17 AAETCTDPVISTSSYTTNDAVISSETVFIVELSLACGNGAQSLALYADVSGRQFPVTRGQDVGKYQVSWSAPHNQAGSGTYQVKFFDEESYSMLRKAQRNNEDVEAIKPLFTVNVDHRGAWNGPWVSTEVLAAVIGIVLYYLAYSSKNSIQA 168          
BLAST of translocon associated protein subunit delta vs. Ensembl Cavefish
Match: ENSAMXT00000034710.1 (translocon-associated protein subunit delta [Source:NCBI gene;Acc:103044981])

HSP 1 Score: 119.783 bits (299), Expect = 6.228e-34
Identity = 56/162 (34.57%), Postives = 98/162 (60.49%), Query Frame = 3
Query:   57 LIFSVIYSV-TATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
            LI ++++ +     C +P +  +TY+T +A++SS +VF+ +++L C      + LYA++NG    V +  +  K+QV++   H++  +  Y +KF+DE++YS +RK QR+ E I  IKPLF++N  H+G   GP VSTE +   +  +V +  FS +  I A
Sbjct:    6 LILALLFCLCNGEGCLDPVITPSTYTTNDAVISSESVFIVELSLACANGVQSVALYADVNGRQFPVTRGQDVGKFQVSWSLPHKQASSGTYQVKFFDEESYSALRKAQRNNEDIDTIKPLFSVNVDHRGAWSGPWVSTEVLAAAIGILVYYFAFSAKTIIQA 167          
BLAST of translocon associated protein subunit delta vs. Ensembl Cavefish
Match: ENSAMXT00000049204.1 (translocon-associated protein subunit delta [Source:NCBI gene;Acc:103044981])

HSP 1 Score: 77.411 bits (189), Expect = 1.059e-17
Identity = 33/106 (31.13%), Postives = 66/106 (62.26%), Query Frame = 3
Query:   57 LIFSVIYSVT-ATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRK 371
            LI ++++ +     C +P +  +TY+T +A++SS +VF+ +++L C      + LYA++NG    V +  +  K+QV++   H++  +  Y +KF+DE++YS +RK
Sbjct:    6 LILALLFCLCNGEGCLDPVITPSTYTTNDAVISSESVFIVELSLACANGVQSVALYADVNGRQFPVTRGQDVGKFQVSWSLPHKQASSGTYQVKFFDEESYSALRK 111          
BLAST of translocon associated protein subunit delta vs. Ensembl Nematostella
Match: EDO34498 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SP86])

HSP 1 Score: 120.168 bits (300), Expect = 1.841e-34
Identity = 57/147 (38.78%), Postives = 93/147 (63.27%), Query Frame = 3
Query:   96 CNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGY-GPIVSTETIVILLSSIVCFCVFSNRNKI 533
            C  P+    +Y++    +SS TVF+T+ +L CD  A  ++LYAE+NG +  V + T+  KYQV++ + H++  +  Y + F DE+ YS  RK QRSG S+ DIKPLFTI+ +H+G G  G  V TE I ++ + ++ +C  + ++K+
Sbjct:   24 CVGPKATSTSYTSTNIFMSSETVFLTEFSLACDNNAKDVSLYAEVNGKVMPVTRSTDNSKYQVSWSEEHKQAKSGVYTINFLDEEGYSNYRKAQRSGGSL-DIKPLFTIDINHKGAGREGLWVQTEFIAVVAALLIWWCANNVKSKL 169          
BLAST of translocon associated protein subunit delta vs. Ensembl Nematostella
Match: EDO29738 (pep supercontig:ASM20922v1:NEMVEscaffold_814:5243:6208:-1 gene:NEMVEDRAFT_v1g143455 transcript:EDO29738 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 92.0485 bits (227), Expect = 3.381e-24
Identity = 44/109 (40.37%), Postives = 72/109 (66.06%), Query Frame = 3
Query:  210 INLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGY-GPIVSTETIVILLSSIVCFCVFSNRNKI 533
            ++LYAE+NG +  V + T+  KYQV++ + H++  +  Y + F DE+ YS  RK QRSG S+ DIKPLFTI+ +H+G G  G  V TE I ++ + ++ +C  + ++K+
Sbjct:    3 VSLYAEVNGKVMPVTRSTDNSKYQVSWSEEHKQAKSGVYTINFLDEEGYSNYRKAQRSGGSL-DIKPLFTIDINHKGAGREGLWVQTEFIAVVAALLIWWCANNVKSKL 110          
BLAST of translocon associated protein subunit delta vs. Ensembl Medaka
Match: ssr4 (signal sequence receptor subunit 4 [Source:NCBI gene;Acc:101165731])

HSP 1 Score: 125.946 bits (315), Expect = 2.464e-36
Identity = 54/149 (36.24%), Postives = 94/149 (63.09%), Query Frame = 3
Query:   93 TCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
            +C +P +  + Y+T +A++SS +VF+ ++ L C   A  + LYA++NG    V +  +  KYQV++   H++  +  Y +KF+DE++YS +RK QR+ E ++ I+PLF++N  H+G   GP VSTE +  L+  ++ +  FS +N I A
Sbjct:   19 SCTDPVITPSAYTTSDAVISSESVFIVELGLTCANGAQTVTLYADVNGRQFPVTRGQDVGKYQVSWSLPHKQASSGTYQVKFFDEESYSALRKAQRNNEDVNAIQPLFSVNIDHRGAWNGPWVSTEVVAALIGILIYYLAFSAKNTIQA 167          
BLAST of translocon associated protein subunit delta vs. Planmine SMEST
Match: SMESG000067948.1 (SMESG000067948.1)

HSP 1 Score: 342.428 bits (877), Expect = 1.061e-121
Identity = 166/167 (99.40%), Postives = 166/167 (99.40%), Query Frame = 3
Query:   39 MIFNVLLIFSVIYSVTATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 539
            MIFNVL IFSVIYSVTATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA
Sbjct:    1 MIFNVLFIFSVIYSVTATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLKCDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKFYDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILLSSIVCFCVFSNRNKILA 167          
The following BLAST results are available for this feature:
BLAST of translocon associated protein subunit delta vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
SSR41.230e-3235.57signal sequence receptor subunit 4 [Source:HGNC Sy... [more]
SSR41.230e-3235.57signal sequence receptor subunit 4 [Source:HGNC Sy... [more]
SSR41.230e-3235.57signal sequence receptor subunit 4 [Source:HGNC Sy... [more]
SSR49.943e-2431.76signal sequence receptor subunit 4 [Source:HGNC Sy... [more]
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BLAST of translocon associated protein subunit delta vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
trap-42.921e-1725.50TRanslocon-Associated Protein [Source:UniProtKB/T... [more]
trap-42.921e-1725.50TRanslocon-Associated Protein [Source:UniProtKB/T... [more]
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BLAST of translocon associated protein subunit delta vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Tapdelta8.924e-1927.54gene:FBgn0021795 transcript:FBtr0345952[more]
Tapdelta8.924e-1927.54gene:FBgn0021795 transcript:FBtr0088153[more]
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BLAST of translocon associated protein subunit delta vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ssr44.434e-3936.09signal sequence receptor, delta [Source:ZFIN;Acc:Z... [more]
ssr41.100e-3738.26signal sequence receptor, delta [Source:ZFIN;Acc:Z... [more]
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BLAST of translocon associated protein subunit delta vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ftsj11.439e-3537.01FtsJ RNA methyltransferase homolog 1 [Source:Xenba... [more]
ftsj12.471e-3536.60FtsJ RNA methyltransferase homolog 1 [Source:Xenba... [more]
ftsj12.471e-3536.60FtsJ RNA methyltransferase homolog 1 [Source:Xenba... [more]
ftsj13.685e-3537.58FtsJ RNA methyltransferase homolog 1 [Source:Xenba... [more]
ftsj15.805e-3537.58FtsJ RNA methyltransferase homolog 1 [Source:Xenba... [more]
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BLAST of translocon associated protein subunit delta vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Ssr45.903e-3434.87signal sequence receptor, delta [Source:MGI Symbol... [more]
Ssr42.459e-3334.90signal sequence receptor, delta [Source:MGI Symbol... [more]
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BLAST of translocon associated protein subunit delta vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q07984|SSRD_RAT7.798e-3334.87Translocon-associated protein subunit delta OS=Rat... [more]
sp|Q62186|SSRD_MOUSE1.720e-3234.90Translocon-associated protein subunit delta OS=Mus... [more]
sp|Q5REH6|SSRD_PONAB5.908e-3235.57Translocon-associated protein subunit delta OS=Pon... [more]
sp|P51571|SSRD_HUMAN5.908e-3235.57Translocon-associated protein subunit delta OS=Hom... [more]
sp|Q2TBX5|SSRD_BOVIN6.000e-3234.90Translocon-associated protein subunit delta OS=Bos... [more]
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BLAST of translocon associated protein subunit delta vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
I1ZII53.817e-11899.40Translocon associated protein subunit delta OS=Sch... [more]
V9XRP22.346e-6899.04TRAPD (Fragment) OS=Schmidtea mediterranea OX=7932... [more]
A0A2C9M9177.682e-4242.11Uncharacterized protein OS=Biomphalaria glabrata O... [more]
K1QFG91.870e-3940.36Translocon-associated protein subunit delta OS=Cra... [more]
A0A3S1CBR45.156e-3936.69Uncharacterized protein OS=Elysia chlorotica OX=18... [more]
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BLAST of translocon associated protein subunit delta vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ssr41.991e-3736.84translocon-associated protein subunit delta-like [... [more]
ENSAMXT00000034710.16.228e-3434.57translocon-associated protein subunit delta [Sourc... [more]
ENSAMXT00000049204.11.059e-1731.13translocon-associated protein subunit delta [Sourc... [more]
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BLAST of translocon associated protein subunit delta vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of translocon associated protein subunit delta vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of translocon associated protein subunit delta vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO344981.841e-3438.78Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO297383.381e-2440.37pep supercontig:ASM20922v1:NEMVEscaffold_814:5243:... [more]
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BLAST of translocon associated protein subunit delta vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ssr42.464e-3636.24signal sequence receptor subunit 4 [Source:NCBI ge... [more]
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BLAST of translocon associated protein subunit delta vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000067948.11.061e-12199.40SMESG000067948.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30015265
GenBankAFJ24839.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015265 ID=SMED30015265|Name=translocon associated protein subunit delta|organism=Schmidtea mediterranea sexual|type=transcript|length=647bp
AACGAATTTCACTGCCACTTCAGTTTTAACGTGTCAAAATGATTTTTAAC
GTTTTATTAATTTTTTCAGTCATTTATTCAGTCACAGCTACAACTTGTAA
TAATCCCCAAGTTGAGCATAATACATATAGTACCAAAGAAGCCTTGCTAT
CTAGTAGAACAGTTTTTGTAACGGATATTACCTTGAAATGTGATGGTCAA
GCTACTAAAATCAATCTGTATGCTGAATTAAATGGACATATTACACTAGT
TGGTAAAGATACAGAAACTGATAAATATCAAGTGACATTTGAAGATCACC
ATCGTAAATTACCAGCAAATAATTATCTTTTAAAATTTTACGACGAAGAC
ACATACAGTGTAATTAGAAAAAATCAAAGATCAGGTGAATCAATCTCAGA
TATTAAGCCATTATTTACTATTAATTTTCATCATCAAGGAGTTGGATATG
GACCTATTGTTAGTACAGAAACCATTGTAATTTTGTTATCATCTATCGTT
TGTTTTTGTGTATTTTCCAATCGTAACAAAATCCTAGCGTAATTGTAGGA
TATTTTTTGAATTTGATATTTAATGTATGATAAATGTATCTTGTCTAATT
TTGATGAATACCGTATAATAAAATTTCCCGGTATTCAATGACTTGAC
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protein sequence of SMED30015265-orf-1

>SMED30015265-orf-1 ID=SMED30015265-orf-1|Name=SMED30015265-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=168bp
MIFNVLLIFSVIYSVTATTCNNPQVEHNTYSTKEALLSSRTVFVTDITLK
CDGQATKINLYAELNGHITLVGKDTETDKYQVTFEDHHRKLPANNYLLKF
YDEDTYSVIRKNQRSGESISDIKPLFTINFHHQGVGYGPIVSTETIVILL
SSIVCFCVFSNRNKILA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR008855TRAP-delta
Vocabulary: cellular component
TermDefinition
GO:0005783endoplasmic reticulum
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008855Translocon-associatedPFAMPF05404TRAP-deltacoord: 6..165
e-value: 6.8E-52
score: 175.3
IPR008855Translocon-associatedPANTHERPTHR12731TRANSLOCON-ASSOCIATED PROTEIN, DELTA SUBUNITcoord: 6..165
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..17
score: 0.866
NoneNo IPR availableTMHMMTMhelixcoord: 137..159