bax-1

Overview
Namebax-1 (AEX93477.1)
Smed IDSMED30015261
Length (bp)1393
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of bax-1 (SMED30015261) t-SNE clustered cells

Violin plots show distribution of expression levels for bax-1 (SMED30015261) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of bax-1 (SMED30015261) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for bax-1 (SMED30015261) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30015261

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
posterior sideSMED30015261h1SMcG0002275 dd_Smed_v4_9696_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30015261h1SMcG0002275 dd_Smed_v4_9696_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of bax-1 vs. Ensembl Human
Match: BAX (BCL2 associated X, apoptosis regulator [Source:HGNC Symbol;Acc:HGNC:959])

HSP 1 Score: 52.7582 bits (125), Expect = 7.973e-8
Identity = 30/102 (29.41%), Postives = 50/102 (49.02%), Query Frame = 2
Query:  677 SDSSDWLRTNALQMNQM----DKIQARQELNKIFLSIFSE-KINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEF 967
            + S   ++T AL +  M    D    R+   ++   +FS+   NWGR+VA+  F  +L   A      +LI   +  ++ F+ E L  WI   GGW+GL+ +
Sbjct:   14 TSSEQIMKTGALLLQGMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSY 115          
BLAST of bax-1 vs. Ensembl Human
Match: BAX (BCL2 associated X, apoptosis regulator [Source:HGNC Symbol;Acc:HGNC:959])

HSP 1 Score: 50.0618 bits (118), Expect = 5.973e-7
Identity = 25/82 (30.49%), Postives = 42/82 (51.22%), Query Frame = 2
Query:  725 MDKIQARQELNKIFLSIFSE-KINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEF 967
            +D    R+   ++   +FS+   NWGR+VA+  F  +L   A      +LI   +  ++ F+ E L  WI   GGW+GL+ +
Sbjct:   17 VDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSY 98          
BLAST of bax-1 vs. Ensembl Human
Match: BAX (BCL2 associated X, apoptosis regulator [Source:HGNC Symbol;Acc:HGNC:959])

HSP 1 Score: 50.8322 bits (120), Expect = 6.567e-7
Identity = 34/130 (26.15%), Postives = 60/130 (46.15%), Query Frame = 2
Query:  593 QCALLPKFDDKFYKSLEHLIKYFEEKFNSDSSDWLRTNALQMNQM----DKIQARQELNKIFLSIFSEK-INWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEF 967
            + AL P   D   K L   +K   ++ +S+         +++ +M    D    R+   ++   +FS+   NWGR+VA+  F  +L   A      +LI   +  ++ F+ E L  WI   GGW+GL+ +
Sbjct:   27 ELALDPVPQDASTKKLSECLKRIGDELDSN---------MELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSY 147          
BLAST of bax-1 vs. Ensembl Human
Match: BAX (BCL2 associated X, apoptosis regulator [Source:HGNC Symbol;Acc:HGNC:959])

HSP 1 Score: 50.447 bits (119), Expect = 1.205e-6
Identity = 34/130 (26.15%), Postives = 60/130 (46.15%), Query Frame = 2
Query:  593 QCALLPKFDDKFYKSLEHLIKYFEEKFNSDSSDWLRTNALQMNQM----DKIQARQELNKIFLSIFSEK-INWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEF 967
            + AL P   D   K L   +K   ++ +S+         +++ +M    D    R+   ++   +FS+   NWGR+VA+  F  +L   A      +LI   +  ++ F+ E L  WI   GGW+GL+ +
Sbjct:   44 ELALDPVPQDASTKKLSECLKRIGDELDSN---------MELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSY 164          
BLAST of bax-1 vs. Ensembl Zebrafish
Match: baxa (BCL2 associated X, apoptosis regulator a [Source:ZFIN;Acc:ZDB-GENE-000511-6])

HSP 1 Score: 56.225 bits (134), Expect = 9.631e-9
Identity = 24/71 (33.80%), Postives = 42/71 (59.15%), Query Frame = 2
Query:  758 KIFLSIFSE-KINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEF 967
            ++   IFS+ K NWGR+VA+  F C L   A      D+I   +  ++S++ E++ +WI + GGW+G+  +
Sbjct:   96 RVAREIFSDGKFNWGRVVALFYFACRLVIKAISTRVPDIIRTIISWTMSYIQEHVINWIREQGGWDGIRSY 166          
BLAST of bax-1 vs. Ensembl Zebrafish
Match: zgc:153993 (zgc:153993 [Source:ZFIN;Acc:ZDB-GENE-060929-176])

HSP 1 Score: 50.447 bits (119), Expect = 8.225e-7
Identity = 23/68 (33.82%), Postives = 38/68 (55.88%), Query Frame = 2
Query:  770 SIFSEKINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEFQT 973
            SIF + INWGR+VA+      L   A   +  ++I+  +   + F+ +++ +WI Q GGWEG+    T
Sbjct:  113 SIFDDGINWGRVVALFHLAYRLIFQALTQNHLEIIKNIISWFLQFMKKHISAWIRQQGGWEGIFRSMT 180          
BLAST of bax-1 vs. Ensembl Xenopus
Match: bax (BCL2-associated X protein [Source:NCBI gene;Acc:394793])

HSP 1 Score: 52.373 bits (124), Expect = 3.047e-7
Identity = 21/66 (31.82%), Postives = 39/66 (59.09%), Query Frame = 2
Query:  773 IFSEKI-NWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEF 967
            +F++ + NWGR+VA+  F C+L   A      ++I   +  ++ ++ EY+  WI   GGWEG++ +
Sbjct:  118 LFADGVFNWGRVVALFYFACKLVVKALCTKVPEMIRTIINWTMEYLREYVVQWIRDQGGWEGMLSY 183          
BLAST of bax-1 vs. Ensembl Xenopus
Match: bax (BCL2-associated X protein [Source:NCBI gene;Acc:394793])

HSP 1 Score: 52.373 bits (124), Expect = 4.064e-7
Identity = 21/66 (31.82%), Postives = 39/66 (59.09%), Query Frame = 2
Query:  773 IFSEKI-NWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEF 967
            +F++ + NWGR+VA+  F C+L   A      ++I   +  ++ ++ EY+  WI   GGWEG++ +
Sbjct:  129 LFADGVFNWGRVVALFYFACKLVVKALCTKVPEMIRTIINWTMEYLREYVVQWIRDQGGWEGMLSY 194          
BLAST of bax-1 vs. Ensembl Mouse
Match: Bax (BCL2-associated X protein [Source:MGI Symbol;Acc:MGI:99702])

HSP 1 Score: 47.7506 bits (112), Expect = 4.735e-6
Identity = 22/59 (37.29%), Postives = 32/59 (54.24%), Query Frame = 2
Query:  791 NWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEF 967
            NWGR+VA+  F  +L   A      +LI   +  ++ F+ E L  WI   GGWEGL+ +
Sbjct:   87 NWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLVWIQDQGGWEGLLSY 145          
BLAST of bax-1 vs. Ensembl Mouse
Match: Bax (BCL2-associated X protein [Source:MGI Symbol;Acc:MGI:99702])

HSP 1 Score: 48.1358 bits (113), Expect = 5.359e-6
Identity = 25/82 (30.49%), Postives = 42/82 (51.22%), Query Frame = 2
Query:  725 MDKIQARQELNKIFLSIFSEK-INWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEF 967
            +D    R+   ++   +F++   NWGR+VA+  F  +L   A      +LI   +  ++ F+ E L  WI   GGWEGL+ +
Sbjct:   83 VDTDSPREVFFRVAADMFADGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLVWIQDQGGWEGLLSY 164          
BLAST of bax-1 vs. UniProt/SwissProt
Match: sp|Q07812|BAX_HUMAN (Apoptosis regulator BAX OS=Homo sapiens OX=9606 GN=BAX PE=1 SV=1)

HSP 1 Score: 50.447 bits (119), Expect = 5.786e-6
Identity = 34/130 (26.15%), Postives = 60/130 (46.15%), Query Frame = 2
Query:  593 QCALLPKFDDKFYKSLEHLIKYFEEKFNSDSSDWLRTNALQMNQM----DKIQARQELNKIFLSIFSEK-INWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEF 967
            + AL P   D   K L   +K   ++ +S+         +++ +M    D    R+   ++   +FS+   NWGR+VA+  F  +L   A      +LI   +  ++ F+ E L  WI   GGW+GL+ +
Sbjct:   44 ELALDPVPQDASTKKLSECLKRIGDELDSN---------MELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSY 164          
BLAST of bax-1 vs. TrEMBL
Match: H2DL15 (Bax-1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 624.394 bits (1609), Expect = 0.000e+0
Identity = 335/336 (99.70%), Postives = 336/336 (100.00%), Query Frame = 2
Query:   86 MGDYDTDDELKNDAHFDDSIPSMESESICDRITNNSSNATPVNNGDDYXXXXXXXXXXXXXXCYTAEVFVKGISYVLKKKIKNCSPSNIPTERKYVNLLSVDDGFQEDNPSPLMSSICSDRDQGALSDASSEGFYCQNDYNHACNEGLCMHVTPFISKLKQNFFLPNNDQCALLPKFDDKFYKSLEHLIKYFEEKFNSDSSDWLRTNALQMNQMDKIQARQELNKIFLSIFSEKINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEFQTGQSGSNNELENIFXXXXXXXXXXXXXXXXXXFCKYVADHI 1093
            MGDYDTDDELKNDAHFDDSIPSME+ESICDRITNNSSNATPVNNGDDYSSSNSHRNHRHNPRCYTAEVFVKGISYVLKKKIKNCSPSNIPTERKYVNLLSVDDGFQEDNPSPLMSSICSDRDQGALSDASSEGFYCQNDYNHACNEGLCMHVTPFISKLKQNFFLPNNDQCALLPKFDDKFYKSLEHLIKYFEEKFNSDSSDWLRTNALQMNQMDKIQARQELNKIFLSIFSEKINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEFQTGQSGSNNELENIFSSLSRIARVAALALAGVAFCKYVADHI
Sbjct:    1 MGDYDTDDELKNDAHFDDSIPSMEAESICDRITNNSSNATPVNNGDDYSSSNSHRNHRHNPRCYTAEVFVKGISYVLKKKIKNCSPSNIPTERKYVNLLSVDDGFQEDNPSPLMSSICSDRDQGALSDASSEGFYCQNDYNHACNEGLCMHVTPFISKLKQNFFLPNNDQCALLPKFDDKFYKSLEHLIKYFEEKFNSDSSDWLRTNALQMNQMDKIQARQELNKIFLSIFSEKINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEFQTGQSGSNNELENIFSSLSRIARVAALALAGVAFCKYVADHI 336          
BLAST of bax-1 vs. TrEMBL
Match: A0A182YTM2 (BH4_2 domain-containing protein OS=Biomphalaria glabrata OX=6526 GN=106067260 PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 9.726e-10
Identity = 39/114 (34.21%), Postives = 62/114 (54.39%), Query Frame = 2
Query:  635 SLEHLIKYFEEKFNSDSSDWLRTNALQMNQMDKIQARQELNKIFLSIFSEKINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEFQTG 976
            ++  L   FEE+F+S   D +     Q+N  D+  A +    I   IFS+ INWGRIVA+ GF    +   + +++  L++  +    ++VD  L SW+  H  W+G MEFQ+G
Sbjct:   64 AMRTLGDEFEERFSSRFDDLIN----QLNINDE-NAYETYTAIVNEIFSDSINWGRIVALFGFSGRFAVRCYELNKTHLVDNLLEWLTTYVDTRLSSWMNTHNNWQGFMEFQSG 172          
BLAST of bax-1 vs. TrEMBL
Match: A0A1D1VDS4 (Uncharacterized protein OS=Ramazzottius varieornatus OX=947166 GN=RvY_10015-1 PE=4 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 1.453e-8
Identity = 37/124 (29.84%), Postives = 71/124 (57.26%), Query Frame = 2
Query:  599 ALLPKFDDKFYKSLEHLIKYFEEKFNSDSSDWLRTNALQMNQMDKIQARQELNKIFLSIFSEKINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEFQ 970
            AL    D ++++S E    +  + F+S++++ LR   L M+Q D     Q++     +++ EKINWGRI+    F  +L++ A +  +++L+ +       F+  +L +WI  HGGWEGL++++
Sbjct:  159 ALGDHLDSRYWQSFEE--AFPADWFDSNTTEELRNRFLDMSQ-DLYSNVQDVED--EALYEEKINWGRIITHFCFTSQLATVAVLSKQYELVNQLTGWLTQFITLHLRTWIEHHGGWEGLVDYR 277          
BLAST of bax-1 vs. TrEMBL
Match: A0A0B7AMK5 (BH4_2 domain-containing protein OS=Arion vulgaris OX=1028688 GN=ORF124635 PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 4.151e-8
Identity = 40/147 (27.21%), Postives = 65/147 (44.22%), Query Frame = 2
Query:  563 KQNFFLPNNDQCALLPKFDDKFYKSLEHLIKYFEEKFNSDSSDWLRTNALQMNQMDKIQARQELNKIFLSIFSEKINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEFQTGQSGSNNELE 1003
            +Q+   PN  Q A+    DD            FEE++NS   D      +Q   +    A      I   IF++ +NWGR++A+ GF    +   F     ++++  V    ++VD  L  W+  H  W+G MEFQ  Q  S  +++
Sbjct:   45 RQSNITPNPVQSAMRRLGDD------------FEERYNSRFVD-----MIQKLNITSDNASDTFTAIMDEIFTDGVNWGRVIALFGFSGRFAVYCFEHDMDEMVDHIVELVTAYVDNTLRDWMLNHNNWQGFMEFQAVQPESEKDIQ 174          
BLAST of bax-1 vs. TrEMBL
Match: A0A2T7PBP0 (BH4_2 domain-containing protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_10104 PE=4 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 5.035e-8
Identity = 42/140 (30.00%), Postives = 63/140 (45.00%), Query Frame = 2
Query:  557 KLKQNFFLPNNDQCALLPKFDDKFYKSLEHLIKYFEEKFNSDSSDWLRTNALQMNQMDKIQARQELNKIFLSIFSEKINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEFQTG 976
            +L++N     N Q  + P   ++  +++  L   FE KF++   D      +   Q+    A Q    I   IF + +NWGRIVA+ G    L+  +       LIE  V    ++VD  L  WI  H GW G +EF  G
Sbjct:   27 RLERNGLAWQNGQRDVTP---NEIQRAMRALGDEFESKFSAQFDD-----MMSQLQLTPDTAYQTFRTIVTEIFHDGVNWGRIVALFGLAGRLAVLSSHQEMPRLIESIVDWVSAYVDTNLKQWITDHDGWAGFLEFYAG 158          
BLAST of bax-1 vs. Ensembl Cavefish
Match: ENSAMXT00000005311.2 (pep primary_assembly:Astyanax_mexicanus-2.0:2:7917921:7922418:1 gene:ENSAMXG00000005183.2 transcript:ENSAMXT00000005311.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.7658 bits (138), Expect = 1.984e-9
Identity = 28/91 (30.77%), Postives = 47/91 (51.65%), Query Frame = 2
Query:  710 LQMNQMDKIQARQELNKIFLSIFSE-----KINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEF 967
            +Q+  M    A Q    +F+ +  E     K NWGR+VA+  F C L   A +    D+I   +  +V ++ E++ +WI + GGWEG+  +
Sbjct:   74 VQLQSMINDSALQPTQDVFVKVACEIFSDGKFNWGRVVALFYFACRLVIKAVLTKLPDIIRTIINWTVDYLREHVINWIREQGGWEGIRSY 164          
BLAST of bax-1 vs. Ensembl Cavefish
Match: zgc:153993 (apoptosis regulator BAX-like [Source:NCBI gene;Acc:103047816])

HSP 1 Score: 51.6026 bits (122), Expect = 2.865e-7
Identity = 21/65 (32.31%), Postives = 41/65 (63.08%), Query Frame = 2
Query:  770 SIFSEKINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLME 964
            +IF++ INWGRIVA+     +L  +A   +  ++I+  +   + ++ +++ +WI Q GGWEG++ 
Sbjct:  113 TIFTDGINWGRIVALFHLAYQLIYNALTQNHLEIIKNIISWVLQYIRQHVSAWIRQQGGWEGVIR 177          
BLAST of bax-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008674.1 (pep scaffold:Pmarinus_7.0:GL477107:593792:611133:1 gene:ENSPMAG00000007850.1 transcript:ENSPMAT00000008674.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.5286 bits (127), Expect = 2.882e-9
Identity = 23/76 (30.26%), Postives = 43/76 (56.58%), Query Frame = 2
Query:  743 RQELNKIFLSIFSE-KINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEF 967
            R+   ++ + IF++   NWGR+VA+  F   L   A+     D+++  +  +V F+ E++  WI + GGWEG+  +
Sbjct:   13 RELFMRVAIQIFADGTFNWGRVVALFYFAYRLVMKAYSRQLLDIVKTIISWAVDFIREHVIQWIREQGGWEGVRTY 88          
BLAST of bax-1 vs. Ensembl Nematostella
Match: EDO33714 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRF5])

HSP 1 Score: 47.7506 bits (112), Expect = 3.929e-7
Identity = 25/73 (34.25%), Postives = 36/73 (49.32%), Query Frame = 2
Query:  740 ARQELNKIFLSIFSEKINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGL 958
            AR     +   IF   INWGRIVA+  F   ++       R +++   V    SF+ + L SWI ++GGW  L
Sbjct:   30 ARSTFFSVAKEIFQTGINWGRIVALFTFGGVVAHHFIESERPEMVPHVVEWIPSFIADNLLSWIMENGGWVSL 102          
BLAST of bax-1 vs. Ensembl Nematostella
Match: EDO47196 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RN20])

HSP 1 Score: 46.2098 bits (108), Expect = 1.494e-6
Identity = 21/79 (26.58%), Postives = 42/79 (53.16%), Query Frame = 2
Query:  731 KIQARQEL--NKIFLS----IFSEKINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGW 949
            KI+ R E+    +F +    +F + ++W +++A+  F   +S    M  R +L++  +    ++V ++L  WI  HGGW
Sbjct:   24 KIRLRSEVIVGDVFRTFGRELFRDGVSWAKVIALFAFAASMSEDCVMQGRPELVKSILNNVRAYVRDHLAVWIRNHGGW 102          
BLAST of bax-1 vs. Ensembl Nematostella
Match: EDO42793 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0T4])

HSP 1 Score: 46.2098 bits (108), Expect = 1.631e-6
Identity = 27/67 (40.30%), Postives = 38/67 (56.72%), Query Frame = 2
Query:  761 IFLSIFSEK-INWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGL 958
            + L IF +  INWGRIV +  F  +L+    ++++  LI+  +   V FV E L  WIFQ GGW  L
Sbjct:   39 VALQIFQDGVINWGRIVTLFYFGYKLALK--VLNQIPLIKMIIEWVVKFVKEKLVKWIFQQGGWVSL 103          
BLAST of bax-1 vs. Ensembl Medaka
Match: baxa (apoptosis regulator BAX [Source:NCBI gene;Acc:101168213])

HSP 1 Score: 55.0694 bits (131), Expect = 1.535e-8
Identity = 23/68 (33.82%), Postives = 40/68 (58.82%), Query Frame = 2
Query:  758 KIFLSIFSEKI-NWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGL 958
            K+ L IFS+ + NWGR+V +  F C L   A +    D+I      ++ ++ +Y+ +WI + GGW+G+
Sbjct:   95 KVALEIFSDGVYNWGRVVTLFYFACRLVIKALITKVPDIIRTIFTWTIDYLRDYVIAWIREQGGWDGI 162          
BLAST of bax-1 vs. Ensembl Medaka
Match: zgc:153993 (apoptosis regulator BAX [Source:NCBI gene;Acc:101168687])

HSP 1 Score: 51.6026 bits (122), Expect = 6.448e-7
Identity = 30/107 (28.04%), Postives = 51/107 (47.66%), Query Frame = 2
Query:  650 IKYFEEKFNSDSSDWLRTNALQ--MNQMDKIQARQELNKIFLSIFSEKINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLME 964
            IK   E+    + D  R   LQ  +NQ     A+    ++  SIF++ INWGR+VA+     +    A   +  + I   +   +  + E L+ W+ Q GGW G+++
Sbjct:  160 IKEVVEQLLKIADDINRNAVLQQLINQASGSGAQDIFMEVARSIFADGINWGRVVALFHLAYKFIYKALTSNHLETIRMIISWVLQVIKEVLYPWLVQQGGWVGVIQ 266          
BLAST of bax-1 vs. Ensembl Medaka
Match: ENSORLT00000046675.1 (bcl-2-like protein 1 [Source:NCBI gene;Acc:101165300])

HSP 1 Score: 48.1358 bits (113), Expect = 6.201e-6
Identity = 31/123 (25.20%), Postives = 55/123 (44.72%), Query Frame = 2
Query:  620 DKFYKSLEHLIKYFEEKFNSDSSDWLRTNALQMNQMDKIQARQELNKIFLSIFSEKINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEFQTGQSGS 988
            D    +L+     FE +F+   SD     ++Q++ +    A Q   ++   +F + INWGR+V +  F   L       +  +L+         ++DE +  WI  HGGW+       GQ+G+
Sbjct:   67 DAIKSTLKDSADEFERRFHQGFSDL----SVQLH-ITPDTAYQNFKRVLDELFKDGINWGRVVGLFVFGGALCVECVERNMGELVSRIAEWMTQYLDEQISPWIHSHGGWDCFARL-YGQNGA 183          
BLAST of bax-1 vs. Planmine SMEST
Match: SMESG000037989.1 (SMESG000037989.1)

HSP 1 Score: 565.844 bits (1457), Expect = 0.000e+0
Identity = 286/288 (99.31%), Postives = 288/288 (100.00%), Query Frame = 2
Query:   86 MGDYDTDDELKNDAHFDDSIPSMESESICDRITNNSSNATPVNNGDDYXXXXXXXXXXXXXXCYTAEVFVKGISYVLKKKIKNCSPSNIPTERKYVNLLSVDDGFQEDNPSPLMSSICSDRDQGALSDASSEGFYCQNDYNHACNEGLCMHVTPFISKLKQNFFLPNNDQCALLPKFDDKFYKSLEHLIKYFEEKFNSDSSDWLRTNALQMNQMDKIQARQELNKIFLSIFSEKINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGW 949
            MGDY+TDDELKNDAHFDDSIPSME+ESICDRITNNSSNATPVNNGDDYSSSNSHRNHRHNPRCYTAEVFVKGISYVLKKKIKNCSPSNIPTERKYVNLLSVDDGFQEDNPSPLMSSICSDRDQGALSDASSEGFYCQNDYNHACNEGLCMHVTPFISKLKQNFFLPNNDQCALLPKFDDKFYKSLEHLIKYFEEKFNSDSSDWLRTNALQMNQMDKIQARQELNKIFLSIFSEKINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGW
Sbjct:    1 MGDYNTDDELKNDAHFDDSIPSMEAESICDRITNNSSNATPVNNGDDYSSSNSHRNHRHNPRCYTAEVFVKGISYVLKKKIKNCSPSNIPTERKYVNLLSVDDGFQEDNPSPLMSSICSDRDQGALSDASSEGFYCQNDYNHACNEGLCMHVTPFISKLKQNFFLPNNDQCALLPKFDDKFYKSLEHLIKYFEEKFNSDSSDWLRTNALQMNQMDKIQARQELNKIFLSIFSEKINWGRIVAMIGFVCELSSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGW 288          
BLAST of bax-1 vs. Planmine SMEST
Match: SMESG000037406.1 (SMESG000037406.1)

HSP 1 Score: 449.514 bits (1155), Expect = 8.413e-159
Identity = 233/234 (99.57%), Postives = 234/234 (100.00%), Query Frame = 2
Query:   86 MGDYDTDDELKNDAHFDDSIPSMESESICDRITNNSSNATPVNNGDDYXXXXXXXXXXXXXXCYTAEVFVKGISYVLKKKIKNCSPSNIPTERKYVNLLSVDDGFQEDNPSPLMSSICSDRDQGALSDASSEGFYCQNDYNHACNEGLCMHVTPFISKLKQNFFLPNNDQCALLPKFDDKFYKSLEHLIKYFEEKFNSDSSDWLRTNALQMNQMDKIQARQELNKIFLSIFSEK 787
            MGDYDTDDELKNDAHFDDSIPSME+ESICDRITNNSSNATPVNNGDDYSSSNSHRNHRHNPRCYTAEVFVKGISYVLKKKIKNCSPSNIPTERKYVNLLSVDDGFQEDNPSPLMSSICSDRDQGALSDASSEGFYCQNDYNHACNEGLCMHVTPFISKLKQNFFLPNNDQCALLPKFDDKFYKSLEHLIKYFEEKFNSDSSDWLRTNALQMNQMDKIQARQELNKIFLSIFSEK
Sbjct:    1 MGDYDTDDELKNDAHFDDSIPSMEAESICDRITNNSSNATPVNNGDDYSSSNSHRNHRHNPRCYTAEVFVKGISYVLKKKIKNCSPSNIPTERKYVNLLSVDDGFQEDNPSPLMSSICSDRDQGALSDASSEGFYCQNDYNHACNEGLCMHVTPFISKLKQNFFLPNNDQCALLPKFDDKFYKSLEHLIKYFEEKFNSDSSDWLRTNALQMNQMDKIQARQELNKIFLSIFSEK 234          
The following BLAST results are available for this feature:
BLAST of bax-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
BAX7.973e-829.41BCL2 associated X, apoptosis regulator [Source:HGN... [more]
BAX5.973e-730.49BCL2 associated X, apoptosis regulator [Source:HGN... [more]
BAX6.567e-726.15BCL2 associated X, apoptosis regulator [Source:HGN... [more]
BAX1.205e-626.15BCL2 associated X, apoptosis regulator [Source:HGN... [more]
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BLAST of bax-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bax-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bax-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
baxa9.631e-933.80BCL2 associated X, apoptosis regulator a [Source:Z... [more]
zgc:1539938.225e-733.82zgc:153993 [Source:ZFIN;Acc:ZDB-GENE-060929-176][more]
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BLAST of bax-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
bax3.047e-731.82BCL2-associated X protein [Source:NCBI gene;Acc:39... [more]
bax4.064e-731.82BCL2-associated X protein [Source:NCBI gene;Acc:39... [more]
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BLAST of bax-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Bax4.735e-637.29BCL2-associated X protein [Source:MGI Symbol;Acc:M... [more]
Bax5.359e-630.49BCL2-associated X protein [Source:MGI Symbol;Acc:M... [more]
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BLAST of bax-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q07812|BAX_HUMAN5.786e-626.15Apoptosis regulator BAX OS=Homo sapiens OX=9606 GN... [more]
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BLAST of bax-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
H2DL150.000e+099.70Bax-1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1[more]
A0A182YTM29.726e-1034.21BH4_2 domain-containing protein OS=Biomphalaria gl... [more]
A0A1D1VDS41.453e-829.84Uncharacterized protein OS=Ramazzottius varieornat... [more]
A0A0B7AMK54.151e-827.21BH4_2 domain-containing protein OS=Arion vulgaris ... [more]
A0A2T7PBP05.035e-830.00BH4_2 domain-containing protein OS=Pomacea canalic... [more]
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BLAST of bax-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSAMXT00000005311.21.984e-930.77pep primary_assembly:Astyanax_mexicanus-2.0:2:7917... [more]
zgc:1539932.865e-732.31apoptosis regulator BAX-like [Source:NCBI gene;Acc... [more]
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BLAST of bax-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000008674.12.882e-930.26pep scaffold:Pmarinus_7.0:GL477107:593792:611133:1... [more]
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BLAST of bax-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bax-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO337143.929e-734.25Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO471961.494e-626.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO427931.631e-640.30Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of bax-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
baxa1.535e-833.82apoptosis regulator BAX [Source:NCBI gene;Acc:1011... [more]
zgc:1539936.448e-728.04apoptosis regulator BAX [Source:NCBI gene;Acc:1011... [more]
ENSORLT00000046675.16.201e-625.20bcl-2-like protein 1 [Source:NCBI gene;Acc:1011653... [more]
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BLAST of bax-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000037989.10.000e+099.31SMESG000037989.1[more]
SMESG000037406.18.413e-15999.57SMESG000037406.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30015261
GenBankAEX93477.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015261 ID=SMED30015261|Name=bax-1|organism=Schmidtea mediterranea sexual|type=transcript|length=1393bp
AATTTAATTTTAATCTACATATAGTAAAATTACATAATTAACAAGTTGTT
GAGTTTTATTCATTATTATCTAGAAAAAGTGTATCATGGGTGATTATGAT
ACAGATGATGAATTAAAAAATGACGCCCATTTTGACGATAGTATTCCTAG
TATGGAGTCGGAATCTATATGTGATCGTATAACCAATAATAGTTCAAACG
CTACTCCAGTTAATAATGGTGACGACTATTCGTCTAGCAATTCTCATAGA
AATCATAGACACAATCCAAGATGTTATACAGCTGAAGTTTTTGTCAAAGG
AATTAGTTACGTATTGAAAAAAAAAATAAAAAATTGTTCTCCAAGCAATA
TACCGACTGAAAGAAAGTATGTAAATTTATTGTCTGTCGATGATGGATTT
CAAGAAGACAATCCTTCACCATTGATGTCGTCAATTTGCAGCGATAGAGA
CCAAGGTGCTCTCTCTGATGCATCGTCTGAAGGATTTTATTGTCAAAATG
ATTACAATCATGCGTGCAATGAAGGACTGTGTATGCATGTTACTCCTTTT
ATTAGTAAACTGAAACAAAATTTCTTTCTTCCTAACAATGACCAATGTGC
ATTACTTCCCAAATTCGATGATAAATTTTACAAGTCTCTCGAACATCTGA
TTAAGTATTTCGAAGAAAAATTTAATTCCGATTCATCCGATTGGTTGCGT
ACAAATGCTTTACAGATGAATCAAATGGATAAAATACAAGCTCGTCAAGA
ACTCAACAAAATATTTCTTTCAATTTTTAGTGAAAAAATCAATTGGGGTC
GAATTGTGGCGATGATTGGATTTGTTTGTGAGCTATCATCTTCAGCATTT
ATGATTCATCGACATGATTTAATCGAGGAATACGTTTACACAAGCGTTTC
ATTTGTCGATGAATATTTATGGAGTTGGATTTTTCAACATGGCGGATGGG
AAGGCTTAATGGAATTCCAAACTGGTCAGAGTGGTTCGAATAACGAGTTG
GAGAATATTTTCAGCAGTTTGAGTAGAATAGCACGAGTAGCAGCTTTAGC
GTTGGCTGGAGTTGCATTTTGTAAATATGTTGCTGATCATATTTAAATAA
AAAGATAAATTGGTCGTACATCCACTATCTACCTCAATTCCAAAATTGTA
AAGAAATCATTTGCTTTTTCATCTGAAATGGGACACATCGTAGAAATTGA
AGATTTTATTTTGAAAGAAACATTTTTCAGCCTGGACAAACATAGTAATG
CATCAGTTGTACAATTGTACACGTACTAGTGAACAAAATATTTTATCAGT
TTCTACCGGATTACTTTTCAAATTTTCAGATTTTCGCTTAACCATTCAAC
TTTAGAGTTATTTTCTTAAAAATTTATTTGATGTCATATTGCT
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protein sequence of SMED30015261-orf-1

>SMED30015261-orf-1 ID=SMED30015261-orf-1|Name=SMED30015261-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=337bp
MGDYDTDDELKNDAHFDDSIPSMESESICDRITNNSSNATPVNNGDDYSS
SNSHRNHRHNPRCYTAEVFVKGISYVLKKKIKNCSPSNIPTERKYVNLLS
VDDGFQEDNPSPLMSSICSDRDQGALSDASSEGFYCQNDYNHACNEGLCM
HVTPFISKLKQNFFLPNNDQCALLPKFDDKFYKSLEHLIKYFEEKFNSDS
SDWLRTNALQMNQMDKIQARQELNKIFLSIFSEKINWGRIVAMIGFVCEL
SSSAFMIHRHDLIEEYVYTSVSFVDEYLWSWIFQHGGWEGLMEFQTGQSG
SNNELENIFSSLSRIARVAALALAGVAFCKYVADHI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR026298Blc2_fam
IPR002475Bcl2-like
Vocabulary: biological process
TermDefinition
GO:0042981regulation of apoptotic process