SMED30015259

Overview
NameSMED30015259
Smed IDSMED30015259
Length (bp)1022
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30015259 (SMED30015259) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30015259 (SMED30015259) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30015259 (SMED30015259) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30015259 (SMED30015259) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30015259

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30015259SMESG000068294.1 SMESG000057064.1 SmedASXL_007675SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
head regionSMED30015259SMESG000040758.1 OX_Smed_1.0.08476ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
zeta neoblastSMED30015259SMESG000068296.1 SMESG000068294.1 SMESG000057066.1 SMESG000057064.1 dd_Smed_v4_59927_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30015259 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001134:28557..33647 +5021
v31.002707:8167..12976 -4987
Homology
[Close]
BLAST of SMED30015259 vs. TrEMBL
Match: A0A504Z505 (Nicastrin OS=Fasciola gigantica OX=46835 GN=FGIG_00979 PE=4 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.478e-13
Identity = 69/323 (21.36%), Postives = 132/323 (40.87%), Query Frame = 3 
Query:  105 ILQFLAICSLMLNEAQKINREIYRYLSPTTYNFCSLRYNYTSKKGCQSYYRGNVGVLYEVRNKNDSLQFFREKFKNYXXXXXXXXXFVDTEIMNKIKITPQINGIVVMEPDTCMAKSCTDEELNDLKLYKVNEYLSCQFYDDCAY---------------AEPYYDLLWPYPILFITVRGKSQTLINECIEKTKQYFNNTSDKRMCGMEIKNPQVEFQSGIECKYFYNRLFVSISWIKKGQ-YCQEYIGNNYYLGXXXXXXXXXXXX----------------XLLYARLDSISMFDTAAYGATQVFPAISIILGI-LNTLKQ 974
            I  +L I  L +         IY YL   + ++CS + N +++ GC S   G+ GVL+   N ND +    E     I+L +   +F++  +M  ++ +P ++G++V  P            L+ + L   +E   C   +   Y                  Y  + WP+P+    V+  +  + N  +    Q+  +  D   C +EI +P     S   C     +  +S+   K+   +C E  G N  L   N  +  +                   +L+ +R+D+ SMF+ + +G+  V P ++++L   ++ L+Q
Sbjct:   11 IFTYLIISLLAVELESSFEESIYHYLPEESLSYCSRKLNISNQVGCSSKLGGSAGVLWMANNSNDVILILSESVSRDIMLALDFHLFINVSLMRDVRSSPLVSGLLVFAP------------LHHVDLLTFSESSGCPNSESSFYGPQRECNVTPMWNPAGSGYSSIDWPFPVAL--VQDPNNLIKNALLSCFSQFNVHPMDDTRCTVEINSPMSAVHSATVCN--RRQYLMSLQVFKEPDVFCDELTGVNIVLSTSNTSHQPSLTADGVTSNNVTTRAPNNSLLVLSRMDARSMFERSGFGSEGVLPGLAVLLSTAVHLLRQ 317
[Close]
BLAST of SMED30015259 vs. TrEMBL
Match: G4V7F3 (Ncstrn_small domain-containing protein OS=Schistosoma mansoni OX=6183 GN=Smp_167240 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.169e-11
Identity = 76/322 (23.60%), Postives = 145/322 (45.03%), Query Frame = 3 
Query:  102 LILQFLAICSLMLNEAQKINREIYRYLSPTTYNFCSLRYNYTSKKGCQSYYRGNVGVLYEVRNKNDSLQFFREKFKNYXXXXXXXXXFVDTEIMNKIKITPQINGIVVMEPD----TCMAKSCTDEELNDL-KLYKVNEYLSCQFYDDCAYAEPYYDLLWPYPILFIT-VRGKSQTLINECIEKTKQYFNNTSDKRMCGMEIKNPQVEFQSGIECKYFYNRLFVSISWIKKGQ-YCQEYIGNNYYLGXXXXX--------------XXXXXXXXLLYARLDSISMFDTAAYGATQVFPAISIILGILNTL--KQTLNGVRFD 998
            L+  F+++ SL   ++ KI  +IY Y+  +  +FC+ + N T++ GC S  +GN GV   +    D +Q  R       ++++++  F++T +M   + T  I G++V   +       A S + +  N L   Y V E        + A  E Y  + WP+P++ +T V     T ++EC     +     +D   C +EI NP     S   C  F  +  +S+   +  + +C E  G N  L   + +                  N  + +  R+DS S+F+ + + +  V P+I++++ +   L  ++TL   +F+
Sbjct:   77 LVFLFISLDSL---DSSKITEQIYNYVPLSYTSFCTRKLNLTAQVGCSSDIKGNSGVALFMNGSQDIIQTLRSNNLTPFVVVVNVGQFMNTSLMRYFRSTTNIKGLIVFSNEEENYDRYAFSESSKCPNSLYSAYNVTEQCDLDTQWNPAGTE-YSYISWPFPVVLVTDVNNTIWTSMHECFSMLNR---EPADDTRCFIEINNPMSAVGSSETC--FRRQYLMSLHISESSEIFCDELTGLNIILSATHSENLSRDNNAVNVSNYVRSENSSVFVLTRMDSRSIFERSGFSSQGVLPSIAVLISVAVHLMGQRTLKDSQFE 389
[Close]
BLAST of SMED30015259 vs. TrEMBL
Match: A0A0V0J209 (Ubiquitin carboxyl-terminal hydrolase isozyme L5 OS=Schistocephalus solidus OX=70667 GN=UCHL5 PE=4 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 4.448e-11
Identity = 74/305 (24.26%), Postives = 141/305 (46.23%), Query Frame = 3 
Query:  165 EIYRYLSPTTYNFCSLRYNYTSKKGCQSYYRGNVGVLYEVRNKNDSLQFFREKFKNYXXXXXXXXXFVDTEIMNKIKITPQINGIVVMEPDTCMAKSCTDEELNDLKL------------YKVNEYLSCQF---YDDCAYAEPYYDLLWPYPILFITVRGKSQTLINECIEKTKQYFNNTSDKRMCGMEIKNPQVEFQSGIECKY--FYNRLFVSISWIKKGQYCQEYIGNNYYLGXXXXXXXXXX------XXXLLYARLDSISMFDTAAYGATQVFPAISIILGILNTLKQTLNGVRFDIYSI 1010
            EIY+Y+  T  +FCS   +Y  + GC S Y  ++G ++ V + + +L      F    I++IS  +F++  ++  ++ + ++ G+VV  P+     +  DEE+ +               +  N+   C+    ++  A+   Y  + WP+P++ I    K   + N+ ++  K+   N++D   C MEI+N    F SGI+     +Y    +S    +  ++C +  G N  +   +K  + N         +L+  R+DS SMFD  A  +  V P ++++  +   L   LN   F  YS+
Sbjct:   20 EIYQYIPQTNVSFCSRSLSYDGQVGCSSKYPSSLGPIF-VADSHSNLPEELSTFTVEHIIVISYHLFLNRSLLFHLRNSKKVAGLVVFAPE-----NPNDEEIKNTDFSENSFCPNGAYNFYNNKTFQCEVTPKWNPTAFD--YATISWPFPVVLIL--DKENHIWNKTLDCYKRLNVNSTDDTRCSMEIRN----FMSGIQSSQTCYYREHLLSYRLEQPTEFCSQIGGVNLMVRAIDKPVLNNTSCRLPNSTLLVLTRIDSRSMFDRKASSSLGVLPGVAVLTSVAAHL---LNTPAFKSYSL 307
[Close]
BLAST of SMED30015259 vs. TrEMBL
Match: A0A4S2LPW9 (Ncstrn_small domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_005975 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 3.488e-10
Identity = 75/308 (24.35%), Postives = 129/308 (41.88%), Query Frame = 3 
Query:  114 FLAICSLM--LNEAQKINREIYRYLSPTTYNFCSLRYNYTSKKGCQSYYRGNVGVLYEVRNKNDSLQFFREKFKNYXXXXXXXXXFVDTEIMNKIKI-TPQINGIVVMEPDTCMAK-----SCTDEELNDLKLYKVNEYLSCQFYDDCAYAEPYYDLLWPYPILFITVRGKSQTLINECIEKTKQYFNNTSDKRMCGMEIKNPQVEFQSGIECKYFYNRLFVSISWIKKGQYCQEYIGNNYYLGXXXXXXX---------------XXXXXXLLYARLDSISMFDTAAYGATQVFPAISIILGILNTL 968
             L  C+++    +   +  +IY Y S  + + CS R N T + GC S  R + GV   V + ++ + F   K     +L++  S+F+++ +MN ++  + +  G+VV+   T   K     S +DE  N L  +   E           +   Y  + WP+P++ I     S  + +E     KQ+  N  D   C +EI+N      S   C    + + + I +     YC E  G N  L   N                     N  +++  RLDS SMF+ +A+ +    P IS+++ I   L
Sbjct:   53 LLTSCTILTCFAQPHHVMGDIYHYFSEQSTSHCSRRLNITGQIGCSSRLRSSSGVALFVEHADEVIDFLNAKNIVPTVLVMQYSLFLNSSLMNDVRSYSERAVGLVVLSQQTSENKHSLPFSESDECPNALYSHYGAERQCSVTPAWNPHGAAYSSIEWPFPVVLIQTADAS--IESELFRCYKQFNANPVDDTRCLIEIRNVMSAISSSALCIRRESLMGLPI-FESPIVYCDELNGVNILLSATNTTASIRKNSPNRSVSVQERSRNSSVVVLTRLDSRSMFERSAFASQGALPGISVLVSIAAHL 357
[Close]
BLAST of SMED30015259 vs. TrEMBL
Match: A0A2H8U0E8 (Nicastrin OS=Melanaphis sacchari OX=742174 GN=Ncstn PE=4 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 6.515e-10
Identity = 67/271 (24.72%), Postives = 115/271 (42.44%), Query Frame = 3 
Query:  198 NFCSLRYNYTSKKGCQSYYRGNVGVLYEVRNKNDSLQFFREKFKNYXXXXXXXXXFVDTEIMNKIKITPQINGIV---------VMEPDTCMAKSCTDEELNDLKLYKVNEYLSCQFYDDCAYAEPYYDLLWPYPILFITVRGKSQTLINECIEKTKQYFNNTSDKRMCGMEIKNPQVEFQSGIECKYFYNRLFVSISWIKKGQYCQEYIGNNYYLGXXXXX-XXXXXXXXLLYARLDSISMFDTAAYGATQVFPAISIILGILNTLKQTL 980
            N C LR N T++ GC S   GNVG L+ V N  D L++   K K     +       + E++  I+++ ++NGI+         V+  D       + E+    +   VN   SC       Y        W +PI  +  +   +++IN C +      NN +D+ +C +E+K+      + ++ K    R  +S   I+  ++C      N Y      + N  N    ++ ARLD+ SMF   A GA     +I  ++   N + + L
Sbjct:   37 NPCFLRLNGTNQFGCTSARFGNVGTLHLVSNSTD-LEWLFNKGKADPYTVALTPKMFNREVLQNIELSTKVNGIMLLMNGTEQEVINNDNLQNNGFSPEDTCPNRYSDVN---SCPIKPWNPYGTDILLKSWSFPIFVVYDQNTIKSIIN-CFKNYNLPLNNQNDQSLCALELKS---HMYAAVDSKTCLRRTMMSYQNIRPIKFCDPLGDKNVYWSLKELRPNTPNQSIAIVAARLDATSMFQDLAPGALSTATSIVTLMTTANLISEML 299
[Close]
BLAST of SMED30015259 vs. Planmine SMEST
Match: SMESG000068294.1 (SMESG000068294.1)

HSP 1 Score: 416.772 bits (1070), Expect = 3.013e-148
Identity = 211/212 (99.53%), Postives = 212/212 (100.00%), Query Frame = 3 
Query:  135 MLNEAQKINREIYRYLSPTTYNFCSLRYNYTSKKGCQSYYRGNVGVLYEVRNKNDSLQFFREKFKNYXXXXXXXXXFVDTEIMNKIKITPQINGIVVMEPDTCMAKSCTDEELNDLKLYKVNEYLSCQFYDDCAYAEPYYDLLWPYPILFITVRGKSQTLINECIEKTKQYFNNTSDKRMCGMEIKNPQVEFQSGIECKYFYNRLFVSISWI 770
            MLNEAQKINREIYRYLSPTTYNFCSLRYNYTSKKGCQSYYRGNVGVLYEVRNKNDSLQFFREKFKNYIILIISISIFVDTEIMNKIKITPQINGIVVMEPDTCMAKSCTDEELNDLKLYKVNEYLSCQFYDDCAYAEPYYDLLWPYPILFITVRGKSQTLINECIEKTKQYFNNTSDKRMCGMEIKNPQVEFQSGIECKYFYNRLFVSISW+
Sbjct:    1 MLNEAQKINREIYRYLSPTTYNFCSLRYNYTSKKGCQSYYRGNVGVLYEVRNKNDSLQFFREKFKNYIILIISISIFVDTEIMNKIKITPQINGIVVMEPDTCMAKSCTDEELNDLKLYKVNEYLSCQFYDDCAYAEPYYDLLWPYPILFITVRGKSQTLINECIEKTKQYFNNTSDKRMCGMEIKNPQVEFQSGIECKYFYNRLFVSISWV 212
[Close]
BLAST of SMED30015259 vs. Planmine SMEST
Match: SMESG000057064.1 (SMESG000057064.1)

HSP 1 Score: 412.92 bits (1060), Expect = 8.226e-147
Identity = 210/212 (99.06%), Postives = 211/212 (99.53%), Query Frame = 3 
Query:  135 MLNEAQKINREIYRYLSPTTYNFCSLRYNYTSKKGCQSYYRGNVGVLYEVRNKNDSLQFFREKFKNYXXXXXXXXXFVDTEIMNKIKITPQINGIVVMEPDTCMAKSCTDEELNDLKLYKVNEYLSCQFYDDCAYAEPYYDLLWPYPILFITVRGKSQTLINECIEKTKQYFNNTSDKRMCGMEIKNPQVEFQSGIECKYFYNRLFVSISWI 770
            ML EAQKINREIYRYLSPTTYNFCSLRYNYTSKKGCQSYYRGNVGVLYEVRNKNDSLQFFREKFKNYIILIISISIFVDTEIMNKIKITPQINGIVVMEPDTCMAKSCTDEELNDLKLYKVNEYLSCQFYDDCAYAEPYYDLLWPYPILFITVRGKSQTLINECIEKTKQYFNNTSDKRMCGMEIKNPQVEFQSGIECKYFYNRLFVSISW+
Sbjct:    1 MLIEAQKINREIYRYLSPTTYNFCSLRYNYTSKKGCQSYYRGNVGVLYEVRNKNDSLQFFREKFKNYIILIISISIFVDTEIMNKIKITPQINGIVVMEPDTCMAKSCTDEELNDLKLYKVNEYLSCQFYDDCAYAEPYYDLLWPYPILFITVRGKSQTLINECIEKTKQYFNNTSDKRMCGMEIKNPQVEFQSGIECKYFYNRLFVSISWV 212
[Close]
BLAST of SMED30015259 vs. Planmine SMEST
Match: SMESG000057064.1 (SMESG000057064.1)

HSP 1 Score: 413.69 bits (1062), Expect = 2.486e-146
Identity = 210/211 (99.53%), Postives = 210/211 (99.53%), Query Frame = 3 
Query:  135 MLNEAQKINREIYRYLSPTTYNFCSLRYNYTSKKGCQSYYRGNVGVLYEVRNKNDSLQFFREKFKNYXXXXXXXXXFVDTEIMNKIKITPQINGIVVMEPDTCMAKSCTDEELNDLKLYKVNEYLSCQFYDDCAYAEPYYDLLWPYPILFITVRGKSQTLINECIEKTKQYFNNTSDKRMCGMEIKNPQVEFQSGIECKYFYNRLFVSISW 767
            ML EAQKINREIYRYLSPTTYNFCSLRYNYTSKKGCQSYYRGNVGVLYEVRNKNDSLQFFREKFKNYIILIISISIFVDTEIMNKIKITPQINGIVVMEPDTCMAKSCTDEELNDLKLYKVNEYLSCQFYDDCAYAEPYYDLLWPYPILFITVRGKSQTLINECIEKTKQYFNNTSDKRMCGMEIKNPQVEFQSGIECKYFYNRLFVSISW
Sbjct:    1 MLIEAQKINREIYRYLSPTTYNFCSLRYNYTSKKGCQSYYRGNVGVLYEVRNKNDSLQFFREKFKNYIILIISISIFVDTEIMNKIKITPQINGIVVMEPDTCMAKSCTDEELNDLKLYKVNEYLSCQFYDDCAYAEPYYDLLWPYPILFITVRGKSQTLINECIEKTKQYFNNTSDKRMCGMEIKNPQVEFQSGIECKYFYNRLFVSISW 211
[Close]
BLAST of SMED30015259 vs. Planmine SMEST
Match: SMESG000035366.1 (SMESG000035366.1)

HSP 1 Score: 88.1965 bits (217), Expect = 6.933e-19
Identity = 82/310 (26.45%), Postives = 146/310 (47.10%), Query Frame = 3 
Query:  102 LILQFLAICSLMLNEAQKINREIYRYLSPT-TYNFCSLRYNYTSKKGCQSYYRGNVGVLYEVRNKND-SLQFFREKFKNYXXXXXXXXXFVDTEIMNKIKITPQINGIVVMEPDTCMAKSCTDEELNDLKLYKVNEYLSCQ--FYDD-----CAYAEPY-----YDLLWPYPILFITVRGKSQTLINECIEKTKQYFNNTSDKRMCGMEIKNPQVEFQSGIEC---KYFYNRLFVSISWIKKG-QYCQEYIGNNYYLGXXXXXXXXXXXXXLLYARLDSISMFDTAAYGATQVFPAISIILGILNTLKQT 977
            ++L  + + S  +N  Q I  E  +   P    +FC+ R N++   GC S   GNVG +  VR+ ++  L+   +K +  I+++ +  +   + + + +     I G+++MEP  C+       +   LKL   +E   C+  FY       C Y   +     Y++ WP+P++F+  +   Q  + +C +    Y     ++  C + I +   + ++   C    Y Y +      W+    QYC +  G N YL F   K    N  IL++ R+D+ SMFDTAA G  ++FP+I I++ I N LK+ 
Sbjct:   11 IVLPNILLLSFFINSIQCI--EFIKLRIPNYATSFCTYRMNFSHSFGCNSKSEGNVGTVLVVRSLSEMKLKLSDQKEEEVILVMPAAMLTRKSTVQDLLDKQHVIAGVIIMEPIYCLINDKRTCKAAILKLKWFSEIDLCKEGFYRHFNQRQCYYNNLFEYYSLYEMRWPFPLVFLKAKENFQQNVYDCYD---TYNAVPGNRNKCKIRISSYTSKPRTSARCIENNYLYRKKL----WLTDDFQYCVDLTGYNIYLTFNPSKKYKENSIILIFTRMDTKSMFDTAAAGCQELFPSIGILIFIGNILKEA 311
[Close]
BLAST of SMED30015259 vs. Planmine SMEST
Match: SMESG000035366.1 (SMESG000035366.1)

HSP 1 Score: 87.8113 bits (216), Expect = 7.122e-19
Identity = 82/310 (26.45%), Postives = 146/310 (47.10%), Query Frame = 3 
Query:  102 LILQFLAICSLMLNEAQKINREIYRYLSPT-TYNFCSLRYNYTSKKGCQSYYRGNVGVLYEVRNKND-SLQFFREKFKNYXXXXXXXXXFVDTEIMNKIKITPQINGIVVMEPDTCMAKSCTDEELNDLKLYKVNEYLSCQ--FYDD-----CAYAEPY-----YDLLWPYPILFITVRGKSQTLINECIEKTKQYFNNTSDKRMCGMEIKNPQVEFQSGIEC---KYFYNRLFVSISWIKKG-QYCQEYIGNNYYLGXXXXXXXXXXXXXLLYARLDSISMFDTAAYGATQVFPAISIILGILNTLKQT 977
            ++L  + + S  +N  Q I  E  +   P    +FC+ R N++   GC S   GNVG +  VR+ ++  L+   +K +  I+++ +  +   + + + +     I G+++MEP  C+       +   LKL   +E   C+  FY       C Y   +     Y++ WP+P++F+  +   Q  + +C +    Y     ++  C + I +   + ++   C    Y Y +      W+    QYC +  G N YL F   K    N  IL++ R+D+ SMFDTAA G  ++FP+I I++ I N LK+ 
Sbjct:   10 IVLPNILLLSFFINSIQCI--EFIKLRIPNYATSFCTYRMNFSHSFGCNSKSEGNVGTVLVVRSLSEMKLKLSDQKEEEVILVMPAAMLTRKSTVQDLLDKQHVIAGVIIMEPIYCLINDKRTCKAAILKLKWFSEIDLCKEGFYRHFNQRQCYYNNLFEYYSLYEMRWPFPLVFLKAKENFQQNVYDCYD---TYNAVPGNRNKCKIRISSYTSKPRTSARCIENNYLYRKKL----WLTDDFQYCVDLTGYNIYLTFNPSKKYKENSIILIFTRMDTKSMFDTAAAGCQELFPSIGILIFIGNILKEA 310
The following BLAST results are available for this feature:
BLAST of SMED30015259 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015259 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015259 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015259 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015259 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015259 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015259 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015259 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A504Z5051.478e-1321.36Nicastrin OS=Fasciola gigantica OX=46835 GN=FGIG_0... [more]
G4V7F31.169e-1123.60Ncstrn_small domain-containing protein OS=Schistos... [more]
A0A0V0J2094.448e-1124.26Ubiquitin carboxyl-terminal hydrolase isozyme L5 O... [more]
A0A4S2LPW93.488e-1024.35Ncstrn_small domain-containing protein OS=Opisthor... [more]
A0A2H8U0E86.515e-1024.72Nicastrin OS=Melanaphis sacchari OX=742174 GN=Ncst... [more]
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BLAST of SMED30015259 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015259 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015259 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015259 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015259 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015259 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000068294.13.013e-14899.53SMESG000068294.1[more]
SMESG000057064.18.226e-14799.06SMESG000057064.1[more]
SMESG000057064.12.486e-14699.53SMESG000057064.1[more]
SMESG000035366.16.933e-1926.45SMESG000035366.1[more]
SMESG000035366.17.122e-1926.45SMESG000035366.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015259 ID=SMED30015259|Name=SMED30015259|organism=Schmidtea mediterranea sexual|type=transcript|length=1022bp
TTCATTTTATATGCTGATATTTTGAAAGTTTTAGCAAGTGTATTAGTAAA
ATAAATGTAACCATTAATTTGTTTCTAAATTTTGTGATGAAAATTAAGTT
CCTAATATTGCAATTTTTAGCAATTTGTAGTTTAATGTTAAATGAAGCTC
AAAAAATCAATCGTGAAATTTATCGTTACCTTTCACCAACAACTTATAAT
TTTTGCAGTTTGAGATACAATTATACTAGCAAGAAAGGATGTCAATCTTA
TTATAGAGGCAATGTTGGTGTTTTGTACGAAGTCCGAAACAAAAATGATT
CACTGCAATTTTTCCGAGAGAAATTTAAAAATTATATCATATTGATCATT
TCTATAAGCATATTTGTTGACACTGAAATTATGAATAAAATTAAAATCAC
TCCTCAAATTAATGGAATTGTAGTAATGGAACCCGATACTTGCATGGCGA
AATCATGCACAGACGAAGAACTTAATGATTTAAAATTATACAAAGTTAAC
GAATATTTAAGTTGTCAATTTTATGACGATTGTGCATATGCCGAACCTTA
TTATGATCTCTTATGGCCATATCCTATTTTATTTATCACAGTTCGTGGGA
AATCTCAAACTCTAATTAATGAATGTATCGAAAAAACAAAACAATACTTT
AACAATACTAGTGATAAAAGAATGTGTGGAATGGAAATAAAAAATCCACA
AGTTGAATTTCAAAGTGGAATAGAATGCAAATATTTTTACAATAGATTAT
TCGTATCTATTTCTTGGATAAAAAAAGGCCAATATTGTCAAGAATATATA
GGAAATAACTATTATTTAGGATTCTTTAACAAAAAAAATATTGTCAATAA
TAAATTTATTTTATTATATGCCAGACTGGACTCTATATCAATGTTTGATA
CTGCTGCCTATGGGGCTACACAGGTTTTCCCAGCAATTTCAATTATACTT
GGAATCCTAAATACTCTCAAACAAACATTAAATGGTGTTAGGTTTGATAT
ATATAGTATATAGTTATTTACA
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protein sequence of SMED30015259-orf-1

>SMED30015259-orf-1 ID=SMED30015259-orf-1|Name=SMED30015259-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=309bp
MKIKFLILQFLAICSLMLNEAQKINREIYRYLSPTTYNFCSLRYNYTSKK
GCQSYYRGNVGVLYEVRNKNDSLQFFREKFKNYIILIISISIFVDTEIMN
KIKITPQINGIVVMEPDTCMAKSCTDEELNDLKLYKVNEYLSCQFYDDCA
YAEPYYDLLWPYPILFITVRGKSQTLINECIEKTKQYFNNTSDKRMCGME
IKNPQVEFQSGIECKYFYNRLFVSISWIKKGQYCQEYIGNNYYLGFFNKK
NIVNNKFILLYARLDSISMFDTAAYGATQVFPAISIILGILNTLKQTLNG
VRFDIYSI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000418head
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR041084Ncstrn_small
IPR008710Nicastrin
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: biological process
TermDefinition
GO:0016485protein processing
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR041084Nicastrin, small lobePFAMPF18266Ncstrn_smallcoord: 39..203
e-value: 6.3E-15
score: 55.6
IPR008710NicastrinPANTHERPTHR21092NICASTRINcoord: 5..283
NoneNo IPR availableCDDcd03881M28_Nicastrincoord: 160..296
e-value: 3.49841E-7
score: 49.3422
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..21
score: 0.833
NoneNo IPR availableTMHMMTMhelixcoord: 272..294