tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Overview
NametRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
Smed IDSMED30015224
Length (bp)1645
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (SMED30015224) t-SNE clustered cells

Violin plots show distribution of expression levels for tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (SMED30015224) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (SMED30015224) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (SMED30015224) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30015224

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30015224h1SMcG0018089 SmedASXL_010320SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30015224h1SMcG0018089 SmedASXL_010320SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Human
Match: CDK5RAP1 (CDK5 regulatory subunit associated protein 1 [Source:HGNC Symbol;Acc:HGNC:15880])

HSP 1 Score: 565.074 bits (1455), Expect = 0.000e+0
Identity = 285/493 (57.81%), Postives = 359/493 (72.82%), Query Frame = 2 
Query:   86 FSNDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSK----LKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLP-FNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSI 1549
                + VY+ETYGCQMN ND+EIA SIL+  GY  T+NL+EA VILL+TC+IRE AE  IW+RL     +  R  +    L+IG+LGCMAERLK ++L N    V+++ GPDAYRDLPRLL   ++G++  NV LSL+ETYAD+ PV+ S+    SAFVSIMRGCDNMC+YCIVPFTRG+ERSR I SIL+EV+ LS+QG+KE+ LLGQNVNS+RD SE      V     T L+ GF+T YK K GG RF  LLD VS+V+ E+RIRFTSPHPKDFPDEVL LI    NICKQ+HLPAQSGSS VL+ M RGY+REAY+ LV  +R  IP V+L+SDFI+GFCGETEEDH  T+SLL+ V+YN  F + YSMR+KT+A+HRL DDV +E+K  R  EL   FR+   + NQ+ +G  QLVL++G SKRS+ +  GRN+ N KVIFP   +   NN  +     P  GDYV VKIT+A+SQTL+G  +CR+++
Sbjct:    6 LGRQRKVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQEADVILLVTCSIREKAEQTIWNRLHQLKALKTRRPRSRVPLRIGILGCMAERLKEEIL-NREKMVDILAGPDAYRDLPRLLAVAESGQQAANVLLSLDETYADVMPVQTSASAT-SAFVSIMRGCDNMCSYCIVPFTRGRERSRPIASILEEVKKLSEQGLKEVTLLGQNVNSFRDNSEVQFNSAV----PTNLSRGFTTNYKTKQGGLRFAHLLDQVSRVDPEMRIRFTSPHPKDFPDEVLQLIHERDNICKQIHLPAQSGSSRVLEAMRRGYSREAYVELVHHIRESIPGVSLSSDFIAGFCGETEEDHVQTVSLLREVQYNMGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITIFREEATKANQTSVGCTQLVLVEGLSKRSATDLCGRNDGNLKVIFPDAEMEDVNNPGLRVRAQP--GDYVLVKITSASSQTLRGHVLCRTTL 490
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Human
Match: CDK5RAP1 (CDK5 regulatory subunit associated protein 1 [Source:HGNC Symbol;Acc:HGNC:15880])

HSP 1 Score: 564.688 bits (1454), Expect = 0.000e+0
Identity = 285/493 (57.81%), Postives = 359/493 (72.82%), Query Frame = 2 
Query:   86 FSNDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSK----LKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLP-FNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSI 1549
                + VY+ETYGCQMN ND+EIA SIL+  GY  T+NL+EA VILL+TC+IRE AE  IW+RL     +  R  +    L+IG+LGCMAERLK ++L N    V+++ GPDAYRDLPRLL   ++G++  NV LSL+ETYAD+ PV+ S+    SAFVSIMRGCDNMC+YCIVPFTRG+ERSR I SIL+EV+ LS+QG+KE+ LLGQNVNS+RD SE      V     T L+ GF+T YK K GG RF  LLD VS+V+ E+RIRFTSPHPKDFPDEVL LI    NICKQ+HLPAQSGSS VL+ M RGY+REAY+ LV  +R  IP V+L+SDFI+GFCGETEEDH  T+SLL+ V+YN  F + YSMR+KT+A+HRL DDV +E+K  R  EL   FR+   + NQ+ +G  QLVL++G SKRS+ +  GRN+ N KVIFP   +   NN  +     P  GDYV VKIT+A+SQTL+G  +CR+++
Sbjct:   96 LGRQRKVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQEADVILLVTCSIREKAEQTIWNRLHQLKALKTRRPRSRVPLRIGILGCMAERLKEEIL-NREKMVDILAGPDAYRDLPRLLAVAESGQQAANVLLSLDETYADVMPVQTSASAT-SAFVSIMRGCDNMCSYCIVPFTRGRERSRPIASILEEVKKLSEQGLKEVTLLGQNVNSFRDNSEVQFNSAV----PTNLSRGFTTNYKTKQGGLRFAHLLDQVSRVDPEMRIRFTSPHPKDFPDEVLQLIHERDNICKQIHLPAQSGSSRVLEAMRRGYSREAYVELVHHIRESIPGVSLSSDFIAGFCGETEEDHVQTVSLLREVQYNMGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITIFREEATKANQTSVGCTQLVLVEGLSKRSATDLCGRNDGNLKVIFPDAEMEDVNNPGLRVRAQP--GDYVLVKITSASSQTLRGHVLCRTTL 580
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Human
Match: CDK5RAP1 (CDK5 regulatory subunit associated protein 1 [Source:HGNC Symbol;Acc:HGNC:15880])

HSP 1 Score: 554.288 bits (1427), Expect = 0.000e+0
Identity = 285/507 (56.21%), Postives = 359/507 (70.81%), Query Frame = 2 
Query:   86 FSNDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSK----LKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQ--------------GIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLP-FNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSI 1549
                + VY+ETYGCQMN ND+EIA SIL+  GY  T+NL+EA VILL+TC+IRE AE  IW+RL     +  R  +    L+IG+LGCMAERLK ++L N    V+++ GPDAYRDLPRLL   ++G++  NV LSL+ETYAD+ PV+ S+    SAFVSIMRGCDNMC+YCIVPFTRG+ERSR I SIL+EV+ LS+Q              G+KE+ LLGQNVNS+RD SE      V     T L+ GF+T YK K GG RF  LLD VS+V+ E+RIRFTSPHPKDFPDEVL LI    NICKQ+HLPAQSGSS VL+ M RGY+REAY+ LV  +R  IP V+L+SDFI+GFCGETEEDH  T+SLL+ V+YN  F + YSMR+KT+A+HRL DDV +E+K  R  EL   FR+   + NQ+ +G  QLVL++G SKRS+ +  GRN+ N KVIFP   +   NN  +     P  GDYV VKIT+A+SQTL+G  +CR+++
Sbjct:   96 LGRQRKVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQEADVILLVTCSIREKAEQTIWNRLHQLKALKTRRPRSRVPLRIGILGCMAERLKEEIL-NREKMVDILAGPDAYRDLPRLLAVAESGQQAANVLLSLDETYADVMPVQTSASAT-SAFVSIMRGCDNMCSYCIVPFTRGRERSRPIASILEEVKKLSEQVFLPPRPPKVLGLQGLKEVTLLGQNVNSFRDNSEVQFNSAV----PTNLSRGFTTNYKTKQGGLRFAHLLDQVSRVDPEMRIRFTSPHPKDFPDEVLQLIHERDNICKQIHLPAQSGSSRVLEAMRRGYSREAYVELVHHIRESIPGVSLSSDFIAGFCGETEEDHVQTVSLLREVQYNMGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITIFREEATKANQTSVGCTQLVLVEGLSKRSATDLCGRNDGNLKVIFPDAEMEDVNNPGLRVRAQP--GDYVLVKITSASSQTLRGHVLCRTTL 594
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Human
Match: CDK5RAP1 (CDK5 regulatory subunit associated protein 1 [Source:HGNC Symbol;Acc:HGNC:15880])

HSP 1 Score: 242.276 bits (617), Expect = 8.880e-73
Identity = 119/204 (58.33%), Postives = 152/204 (74.51%), Query Frame = 2 
Query:   86 FSNDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSK----LKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEI 685
                + VY+ETYGCQMN ND+EIA SIL+  GY  T+NL+EA VILL+TC+IRE AE  IW+RL     +  R  +    L+IG+LGCMAERLK ++L N    V+++ GPDAYRDLPRLL   ++G++  NV LSL+ETYAD+ PV+ S+    SAFVSIMRGCDNMC+YCIVPFTRG+ERSR I SIL+EV+ LS+Q ++ I
Sbjct:   96 LGRQRKVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQEADVILLVTCSIREKAEQTIWNRLHQLKALKTRRPRSRVPLRIGILGCMAERLKEEIL-NREKMVDILAGPDAYRDLPRLLAVAESGQQAANVLLSLDETYADVMPVQTSASAT-SAFVSIMRGCDNMCSYCIVPFTRGRERSRPIASILEEVKKLSEQVLQLI 297

HSP 2 Score: 234.958 bits (598), Expect = 4.755e-70
Identity = 118/219 (53.88%), Postives = 155/219 (70.78%), Query Frame = 2 
Query:  896 KDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLP-FNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSI 1549
            K   ++VL LI    NICKQ+HLPAQSGSS VL+ M RGY+REAY+ LV  +R  IP V+L+SDFI+GFCGETEEDH  T+SLL+ V+YN  F + YSMR+KT+A+HRL DDV +E+K  R  EL   FR+   + NQ+ +G  QLVL++G SKRS+ +  GRN+ N KVIFP   +   NN  +     P  GDYV VKIT+A+SQTL+G  +CR+++
Sbjct:  287 KKLSEQVLQLIHERDNICKQIHLPAQSGSSRVLEAMRRGYSREAYVELVHHIRESIPGVSLSSDFIAGFCGETEEDHVQTVSLLREVQYNMGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITIFREEATKANQTSVGCTQLVLVEGLSKRSATDLCGRNDGNLKVIFPDAEMEDVNNPGLRVRAQP--GDYVLVKITSASSQTLRGHVLCRTTL 503
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Human
Match: CDK5RAP1 (CDK5 regulatory subunit associated protein 1 [Source:HGNC Symbol;Acc:HGNC:15880])

HSP 1 Score: 188.734 bits (478), Expect = 1.698e-55
Identity = 97/197 (49.24%), Postives = 134/197 (68.02%), Query Frame = 2 
Query:  962 LPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLP-FNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSI 1549
            +P Q+ +S     +   Y+REAY+ LV  +R  IP V+L+SDFI+GFCGETEEDH  T+SLL+ V+YN  F + YSMR+KT+A+HRL DDV +E+K  R  EL   FR+   + NQ+ +G  QLVL++G SKRS+ +  GRN+ N KVIFP   +   NN  +     P  GDYV VKIT+A+SQTL+G  +CR+++
Sbjct:   58 MPVQTSASATSAFV---YSREAYVELVHHIRESIPGVSLSSDFIAGFCGETEEDHVQTVSLLREVQYNMGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITIFREEATKANQTSVGCTQLVLVEGLSKRSATDLCGRNDGNLKVIFPDAEMEDVNNPGLRVRAQP--GDYVLVKITSASSQTLRGHVLCRTTL 249

HSP 2 Score: 78.1814 bits (191), Expect = 1.427e-15
Identity = 40/72 (55.56%), Postives = 53/72 (73.61%), Query Frame = 2 
Query:  335 CMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFV 550
            CMAERLK ++L N    V+++ GPDAYRDLPRLL   ++G++  NV LSL+ETYAD+ PV+ S+    SAFV
Sbjct:    2 CMAERLKEEIL-NREKMVDILAGPDAYRDLPRLLAVAESGQQAANVLLSLDETYADVMPVQTSASAT-SAFV 71
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Celegans
Match: F25B5.5 (CDK5RAP1-like protein [Source:UniProtKB/Swiss-Prot;Acc:Q09316])

HSP 1 Score: 510.76 bits (1314), Expect = 5.154e-177
Identity = 258/503 (51.29%), Postives = 342/503 (67.99%), Query Frame = 2 
Query:   41 KKYLHYSNIQRLCSNFSNDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSI 1549
            K+YL+  ++Q       N + V   TYGCQMN +D EI  SI+   G+  +   + A ++LLMTC+IR+GAE K+W++LK     +    ++ +GVLGCMAER++  LLE     VN+V GPD+YRDLPRL+     G  G+NV LSL+ETYAD+ P+R+ S    +AF+SIMRGCDNMCTYC+VPFTRG+ERSR I+SI++EVQ L DQG K++ LLGQNVNSYRDM+ S +     S  +     GF TVYKPK+GG  F  LL+ V+    +IR RFTSPHPKDFP +++ LI + PN+CKQLHLPAQSG    L+ M RGYTR+ YL LVD +R ++P+V+LTSDFI+GFCGETE+ HQ TLSL++ VRY+F F +PYSMR KT+A HRL DDV +++K+ RHL+L   FR+  L++NQ+ IGS Q VL++G SKR ++   GR +   K +F    L             + G Y  + IT A SQTLK   I +SSI
Sbjct:   66 KEYLNPEDLQ------GNGRTVCYVTYGCQMNVSDMEIVRSIMTKYGFVESDKKENADIVLLMTCSIRDGAEKKVWNQLKLIRSNSVNKGQI-VGVLGCMAERVRHDLLEKRNL-VNIVAGPDSYRDLPRLVAVAAGGSNGINVQLSLDETYADVQPIRVDSAS-KTAFISIMRGCDNMCTYCVVPFTRGRERSRPIESIVEEVQRLRDQGYKQVTLLGQNVNSYRDMT-SMDFSMAPSTSQEDRVPGFKTVYKPKSGGLTFTTLLEKVADAAPDIRFRFTSPHPKDFPMQLIELIASRPNLCKQLHLPAQSGDDETLERMERGYTRDLYLRLVDDIRHVLPSVSLTSDFIAGFCGETEQAHQNTLSLIRAVRYSFCFVFPYSMRGKTRAHHRLTDDVPEDVKARRHLDLTTVFREEALKLNQALIGSEQTVLLEGKSKRDASFSHGRIDGGVKAVFDNSKLCL-----------EPGQYAKILITDANSQTLKAQLIGQSSI 547
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Celegans
Match: Y92H12BL.1 (Threonylcarbamoyladenosine tRNA methylthiotransferase [Source:UniProtKB/Swiss-Prot;Acc:Q8MXQ7])

HSP 1 Score: 89.3521 bits (220), Expect = 6.572e-19
Identity = 80/335 (23.88%), Postives = 150/335 (44.78%), Query Frame = 2 
Query:  548 VSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQ-TLSDQGIKEIVLLGQNVNSY-RDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKE---IRIRFTS-PHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFH-RLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDP 1531
            +SI  GC N CTYC     RG   S  +  ++++ +    D+G+KE+ L  +++ ++ RD+                              G    DLL  + +V  +   +R+  T+ P+  D  +E+  ++   P +   LH+P QS S  VL +M R Y+R  +  + D +   +PN+ + +D I  F  ET ED + ++ L++  ++  +F   Y  R  T A   + ID V    ++    EL  ++ + T E     IG +  VL+   +    +  VG N++  +++ P              E  ++G+++ V++TA T  ++   P
Sbjct:   68 LSISTGCLNNCTYCKTKMARGDLVSYPLADLVEQARAAFHDEGVKELWLTSEDLGAWGRDI------------------------------GLVLPDLLRELVKVIPDGSMMRLGMTNPPYILDHLEEIAEILN-HPKVYAFLHIPVQSASDAVLNDMKREYSRRHFEQIADYMIANVPNIYIATDMILAFPTETLEDFEESMELVRKYKFPSLFINQYYPRSGTPAARLKKIDTVEARKRTAAMSELFRSYTRYTDE----RIGELHRVLVTEVAADKLHG-VGHNKSYEQILVP-------------LEYCKMGEWIEVRVTAVTKFSMISKP 353
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Celegans
Match: Y92H12BL.1 (Threonylcarbamoyladenosine tRNA methylthiotransferase [Source:UniProtKB/Swiss-Prot;Acc:Q8MXQ7])

HSP 1 Score: 89.3521 bits (220), Expect = 6.572e-19
Identity = 80/335 (23.88%), Postives = 150/335 (44.78%), Query Frame = 2 
Query:  548 VSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQ-TLSDQGIKEIVLLGQNVNSY-RDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKE---IRIRFTS-PHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFH-RLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDP 1531
            +SI  GC N CTYC     RG   S  +  ++++ +    D+G+KE+ L  +++ ++ RD+                              G    DLL  + +V  +   +R+  T+ P+  D  +E+  ++   P +   LH+P QS S  VL +M R Y+R  +  + D +   +PN+ + +D I  F  ET ED + ++ L++  ++  +F   Y  R  T A   + ID V    ++    EL  ++ + T E     IG +  VL+   +    +  VG N++  +++ P              E  ++G+++ V++TA T  ++   P
Sbjct:   68 LSISTGCLNNCTYCKTKMARGDLVSYPLADLVEQARAAFHDEGVKELWLTSEDLGAWGRDI------------------------------GLVLPDLLRELVKVIPDGSMMRLGMTNPPYILDHLEEIAEILN-HPKVYAFLHIPVQSASDAVLNDMKREYSRRHFEQIADYMIANVPNIYIATDMILAFPTETLEDFEESMELVRKYKFPSLFINQYYPRSGTPAARLKKIDTVEARKRTAAMSELFRSYTRYTDE----RIGELHRVLVTEVAADKLHG-VGHNKSYEQILVP-------------LEYCKMGEWIEVRVTAVTKFSMISKP 353
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Fly
Match: CG6345 (gene:FBgn0037816 transcript:FBtr0082258)

HSP 1 Score: 533.487 bits (1373), Expect = 0.000e+0
Identity = 267/496 (53.83%), Postives = 350/496 (70.56%), Query Frame = 2 
Query:   92 NDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGI-NKRSSK---LKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKA-GRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSIATFNS 1564
            N + V+ E YGCQMN ND+E+  SILK +GY      +EA VI+L+TCA+R+GAE +I +RLK+   + NKRS++   L++ +LGCMAERLK KLLE     V+++ GPD+Y+DLPRLL   +  G   +NV LSL+ETYAD+ PVRL+S+  P+AFVSIMRGCDNMCTYCIVPFTRG+ERSR + SI+ EV+ L++QG+KE+ LLGQNVNSYRD +   E   +    K     GFSTVYKPKTGG  F  LL +V+Q   ++RIRFTSPHPKDF DEVL +IR  PN+CKQLHLPAQSG++ VL+ M RGY+REAYL LV  +R  +PNV L+SDFI GFCGETEE+ Q T+SL++ V+YN  + + YSMREKT A  R  DDV   +K+ R   +   FR+   ++++   G  QL+LI+G SKRS   W GRN+AN KVI P + +P + D         +GD++AV+I  + SQ LKG P+  S+I  F+S
Sbjct:   90 NGRKVHFEVYGCQMNTNDTEVVFSILKENGYLRCQEPEEADVIMLVTCAVRDGAEQRIRNRLKHLRAMKNKRSTRRHPLQLTLLGCMAERLKEKLLEQEQC-VDVIAGPDSYKDLPRLLAISRHYGNSAINVLLSLDETYADVMPVRLNSES-PTAFVSIMRGCDNMCTYCIVPFTRGRERSRPLASIVAEVKALAEQGVKEVTLLGQNVNSYRDRTAQEEQDSL----KATPVPGFSTVYKPKTGGTPFAALLRSVAQAVPDMRIRFTSPHPKDFSDEVLEVIRDHPNVCKQLHLPAQSGNTQVLERMRRGYSREAYLELVQHIRQFLPNVGLSSDFICGFCGETEEEFQDTVSLIQQVQYNVAYLFAYSMREKTTAHRRYKDDVPINVKNERLQRMVQVFREGATQLHRKMEGQEQLILIEGKSKRSDAHWFGRNDANIKVIVPSIYVPISGDSTARKSF-GVGDFLAVRIEESNSQVLKGTPLELSNITDFHS 578
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Fly
Match: CG6550 (gene:FBgn0034214 transcript:FBtr0086961)

HSP 1 Score: 112.849 bits (281), Expect = 4.331e-26
Identity = 118/487 (24.23%), Postives = 216/487 (44.35%), Query Frame = 2 
Query:  104 VYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSA-------FVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSY-RDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKE---IRIRFTSP-HPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGD 1528
            V+++T+GC  N +DSE     L + GY   +  +EA + LL +C ++  +ED   + ++       R+ K  + V GC+ +        N ++ + +       + + R+++ ++   KG +V L   +       V  +   +P          +SI  GC N CTYC     RG   S   + +++  +    +G  EI L  ++  +Y RD+                              G    +LL  + +V  E   +R+  T+P +  +  +EV ++++  P +   LH+P QSGS +VL  M R Y R+ + ++VD +R  +P V + +D I GF  ETE+D + T++L    R+  +F   +  R  T A    +D +   +   R   L   F   + E     +G I  VL+   S    + +VG N++  +V+     LP  ++L        +G  V V+IT+A+  ++ G+
Sbjct:   73 VFVKTWGCAHNNSDSEYMAGQLAAYGY-RLSGKEEADLWLLNSCTVKNPSEDTFRNEIES----GMRNGK-HVVVAGCVPQGAPKSDYLNGLSVIGV-------QQIDRVVEVVEETLKGHSVQLLQNKKKVHGRRVAGAPLSLPKVRKNPLIEIISINSGCLNQCTYCKTKHARGDLASYPPEEVVERARQSFAEGCCEIWLTSEDTGAYGRDI------------------------------GSSLPELLWQLVEVIPEHCMLRVGMTNPPYILEHLEEVANVLQH-PRVYSFLHVPVQSGSDSVLGEMKREYCRQDFEHVVDFLRERVPGVTIATDIICGFPTETEDDFEETMTLCAKYRFPSLFINQFFPRPGTPAAK--MDRIPANLVKKRTKRLTDLF--YSYEPYADRVGEIYTVLVTEVSHDKLH-YVGHNKSYEQVL-----LPMRDNL--------LGTRVHVRITSASKFSMVGE 497
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Zebrafish
Match: cdk5rap1 (CDK5 regulatory subunit associated protein 1 [Source:ZFIN;Acc:ZDB-GENE-070424-90])

HSP 1 Score: 568.926 bits (1465), Expect = 0.000e+0
Identity = 290/490 (59.18%), Postives = 359/490 (73.27%), Query Frame = 2 
Query:   92 NDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSK----LKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSI 1549
            + + VY ETYGCQMN ND+EIA SIL+  GY+ T  L++A V+LL+TC+IRE AE  IW+RLK+     KR  K    L IGVLGCMAERLKS+LL+     V+++ GPDAYRDLPRLL     G++  NV LSLEETYAD+ PV  + +G  SAFVSIMRGCDNMC+YCIVPFTRG+ERSR + SI++EV+ LSDQG+KEI LLGQNVNSYRD+S+   +    S+  T+L+ GF +VY+ K GG RF DLLD VS +N  +RIRFTSPHPKDFPDEVL LI+   NICK LHLPAQSGSS +L  M RGYTREAYL LV+ +R IIP V+L+SDFI+GFC ETEEDHQ T+SLLK V YN  F + YSMR+KT A+HRL D+V   +K  R  EL + FR+   ++N + IG+ QLVL++G SKRSS E  GRN+ N KVIFPK +LP   +    + I   GDYV VKIT+A SQ+L+G  +  SS+
Sbjct:   88 HSRKVYFETYGCQMNTNDTEIAWSILQKAGYSRTLELQKADVVLLVTCSIREKAEQTIWNRLKHLTASKKRIQKHKRSLTIGVLGCMAERLKSELLDQE-KLVDVLAGPDAYRDLPRLLSLAHGGQRASNVLLSLEETYADVIPVHQTLEG-HSAFVSIMRGCDNMCSYCIVPFTRGRERSRPMSSIVEEVRMLSDQGVKEITLLGQNVNSYRDLSK---LQFSCSERNTQLSRGFQSVYRTKQGGLRFADLLDRVSLINPNMRIRFTSPHPKDFPDEVLQLIQERSNICKHLHLPAQSGSSRILHAMRRGYTREAYLELVENIRKIIPGVSLSSDFIAGFCSETEEDHQQTISLLKEVGYNIGFFFTYSMRKKTHAYHRLEDNVPAPVKKRRLEELISVFREKATKVNMTLIGNTQLVLVEGKSKRSSEELCGRNDGNVKVIFPKTNLPVQPEKTQMACITP-GDYVLVKITSANSQSLRGHALNHSSL 571
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Zebrafish
Match: cdk5rap1 (CDK5 regulatory subunit associated protein 1 [Source:ZFIN;Acc:ZDB-GENE-070424-90])

HSP 1 Score: 568.926 bits (1465), Expect = 0.000e+0
Identity = 290/490 (59.18%), Postives = 359/490 (73.27%), Query Frame = 2 
Query:   92 NDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSK----LKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSI 1549
            + + VY ETYGCQMN ND+EIA SIL+  GY+ T  L++A V+LL+TC+IRE AE  IW+RLK+     KR  K    L IGVLGCMAERLKS+LL+     V+++ GPDAYRDLPRLL     G++  NV LSLEETYAD+ PV  + +G  SAFVSIMRGCDNMC+YCIVPFTRG+ERSR + SI++EV+ LSDQG+KEI LLGQNVNSYRD+S+   +    S+  T+L+ GF +VY+ K GG RF DLLD VS +N  +RIRFTSPHPKDFPDEVL LI+   NICK LHLPAQSGSS +L  M RGYTREAYL LV+ +R IIP V+L+SDFI+GFC ETEEDHQ T+SLLK V YN  F + YSMR+KT A+HRL D+V   +K  R  EL + FR+   ++N + IG+ QLVL++G SKRSS E  GRN+ N KVIFPK +LP   +    + I   GDYV VKIT+A SQ+L+G  +  SS+
Sbjct:   88 HSRKVYFETYGCQMNTNDTEIAWSILQKAGYSRTLELQKADVVLLVTCSIREKAEQTIWNRLKHLTASKKRIQKHKRSLTIGVLGCMAERLKSELLDQE-KLVDVLAGPDAYRDLPRLLSLAHGGQRASNVLLSLEETYADVIPVHQTLEG-HSAFVSIMRGCDNMCSYCIVPFTRGRERSRPMSSIVEEVRMLSDQGVKEITLLGQNVNSYRDLSK---LQFSCSERNTQLSRGFQSVYRTKQGGLRFADLLDRVSLINPNMRIRFTSPHPKDFPDEVLQLIQERSNICKHLHLPAQSGSSRILHAMRRGYTREAYLELVENIRKIIPGVSLSSDFIAGFCSETEEDHQQTISLLKEVGYNIGFFFTYSMRKKTHAYHRLEDNVPAPVKKRRLEELISVFREKATKVNMTLIGNTQLVLVEGKSKRSSEELCGRNDGNVKVIFPKTNLPVQPEKTQMACITP-GDYVLVKITSANSQSLRGHALNHSSL 571
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Zebrafish
Match: cdk5rap1 (CDK5 regulatory subunit associated protein 1 [Source:ZFIN;Acc:ZDB-GENE-070424-90])

HSP 1 Score: 567.77 bits (1462), Expect = 0.000e+0
Identity = 290/489 (59.30%), Postives = 358/489 (73.21%), Query Frame = 2 
Query:   92 NDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSK----LKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSS 1546
            + + VY ETYGCQMN ND+EIA SIL+  GY+ T  L++A V+LL+TC+IRE AE  IW+RLK+     KR  K    L IGVLGCMAERLKS+LL+     V+++ GPDAYRDLPRLL     G++  NV LSLEETYAD+ PV  + +G  SAFVSIMRGCDNMC+YCIVPFTRG+ERSR + SI++EV+ LSDQG+KEI LLGQNVNSYRD+S+   +    S+  T+L+ GF +VY+ K GG RF DLLD VS +N  +RIRFTSPHPKDFPDEVL LI+   NICK LHLPAQSGSS +L  M RGYTREAYL LV+ +R IIP V+L+SDFI+GFC ETEEDHQ T+SLLK V YN  F + YSMR+KT A+HRL D+V   +K  R  EL + FR+   ++N + IG+ QLVL++G SKRSS E  GRN+ N KVIFPK +LP   +    + I   GDYV VKIT+A SQ+L+G  +  SS
Sbjct:   88 HSRKVYFETYGCQMNTNDTEIAWSILQKAGYSRTLELQKADVVLLVTCSIREKAEQTIWNRLKHLTASKKRIQKHKRSLTIGVLGCMAERLKSELLDQE-KLVDVLAGPDAYRDLPRLLSLAHGGQRASNVLLSLEETYADVIPVHQTLEG-HSAFVSIMRGCDNMCSYCIVPFTRGRERSRPMSSIVEEVRMLSDQGVKEITLLGQNVNSYRDLSK---LQFSCSERNTQLSRGFQSVYRTKQGGLRFADLLDRVSLINPNMRIRFTSPHPKDFPDEVLQLIQERSNICKHLHLPAQSGSSRILHAMRRGYTREAYLELVENIRKIIPGVSLSSDFIAGFCSETEEDHQQTISLLKEVGYNIGFFFTYSMRKKTHAYHRLEDNVPAPVKKRRLEELISVFREKATKVNMTLIGNTQLVLVEGKSKRSSEELCGRNDGNVKVIFPKTNLPVQPEKTQMACITP-GDYVLVKITSANSQSLRGHALNHSS 570
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Zebrafish
Match: cdkal1 (CDK5 regulatory subunit associated protein 1 like 1 [Source:HGNC Symbol;Acc:HGNC:21050])

HSP 1 Score: 79.7221 bits (195), Expect = 2.998e-16
Identity = 60/210 (28.57%), Postives = 97/210 (46.19%), Query Frame = 2 
Query:  926 IRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSIAT 1555
            I   P +   LH+P QS S +VL  M R Y    + +LVD ++  +P + + +D I GF GET+ED + TL+L++  R+  +F   +  R  T A   L+  +   +K  R  EL+A F   +       +G  Q VL+   S   S  +V  N+   +V+ PK             E   +G  V V++       +KG P+  + + T
Sbjct:    6 ILQHPRVFSFLHVPLQSASDSVLMEMRREYCCADFTHLVDYLKERVPGITIATDIICGFPGETDEDFEQTLALVRRYRFPSLFINQFYPRPGTPA--ALMQQLPAHVKKQRTKELSALFH--SYRPYDHKMGEQQQVLVTEES-FDSQYYVAHNKFYEQVLVPK-----------RPEY--LGKMVQVEVYECGKHYMKGRPLEEAPLRT 197
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Xenopus
Match: cdk5rap1 (CDK5 regulatory subunit associated protein 1 [Source:NCBI gene;Acc:549946])

HSP 1 Score: 556.599 bits (1433), Expect = 0.000e+0
Identity = 282/485 (58.14%), Postives = 353/485 (72.78%), Query Frame = 2 
Query:   89 SNDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSK---LKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIF---PKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKG 1525
            S +  +Y+ETYGCQMN ND+EIA SIL+ +GY  T    EA VILL+TC++RE AE  IW+RL+    + ++ SK   ++IG+LGCMAERLK ++L+     V+LV GPDAYRDLPRLL   + G++  NV LS++ETYADI PV  S+    SAFVSIMRGCDNMCTYCIVPFTRG+ERSR + SIL+EV+ LS QG+KE+ LLGQNVNSYRD S +       + E   L+ GFST+YK K GG RFVDLLD VSQ++ E+RIRFTSPHPKDFPDEVL LI    NICKQ+H+PAQSGS+ VL+ M RGY+RE YL+L+  ++  IP V+L+SDFI+GFC ETEEDH  TLSLL+ V YN  F + YSMR+KT+A+HRL DDV  E+K  R  EL A FR+    +NQ  +GS QLVL++G SKRS  E  GRN+ NTKVIF   PK+      + + N    Q GDYV VKIT+++SQ+LKG
Sbjct:  134 SRNNKIYLETYGCQMNVNDTEIAWSILQQNGYVRTYTEAEADVILLVTCSVREKAEQTIWNRLQQLAALKRKRSKTSRMRIGLLGCMAERLKQEILDKENL-VDLVAGPDAYRDLPRLLSVAETGQQVANVLLSVDETYADILPVHTSASS-KSAFVSIMRGCDNMCTYCIVPFTRGRERSRPLQSILEEVRMLSSQGVKEVTLLGQNVNSYRDTSSAQ----FPNAESPPLSRGFSTIYKHKKGGLRFVDLLDKVSQIDPEMRIRFTSPHPKDFPDEVLQLIAERHNICKQIHIPAQSGSNKVLETMRRGYSREVYLDLIQNIQESIPGVSLSSDFITGFCEETEEDHLQTLSLLRTVSYNAAFLFAYSMRKKTRAYHRLQDDVPQEVKQRRLAELIAVFREEAGRVNQRAVGSTQLVLVEGPSKRSPEELCGRNDGNTKVIFSEAPKVGGLAQENSLQNR--IQSGDYVLVKITSSSSQSLKG 610
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Xenopus
Match: cdk5rap1 (CDK5 regulatory subunit associated protein 1 [Source:NCBI gene;Acc:549946])

HSP 1 Score: 495.738 bits (1275), Expect = 3.640e-170
Identity = 256/442 (57.92%), Postives = 320/442 (72.40%), Query Frame = 2 
Query:  224 LMTCAI--REGAEDKIWDRLKYYHGINKRSSK---LKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIF---PKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKG 1525
            L TC+I  +E AE  IW+RL+    + ++ SK   ++IG+LGCMAERLK ++L+     V+LV GPDAYRDLPRLL   + G++  NV LS++ETYADI PV  S+    SAFVSIMRGCDNMCTYCIVPFTRG+ERSR + SIL+EV+ LS QG+KE+ LLGQNVNSYRD S +       + E   L+ GFST+YK K GG RFVDLLD VSQ++ E+RIRFTSPHPKDFPDEVL LI    NICKQ+H+PAQSGS+ VL+ M RGY+RE YL+L+  ++  IP V+L+SDFI+GFC ETEEDH  TLSLL+ V YN  F + YSMR+KT+A+HRL DDV  E+K  R  EL A FR+    +NQ  +GS QLVL++G SKRS  E  GRN+ NTKVIF   PK+      + + N    Q GDYV VKIT+++SQ+LKG
Sbjct:  141 LHTCSISCKEKAEQTIWNRLQQLAALKRKRSKTSRMRIGLLGCMAERLKQEILDKENL-VDLVAGPDAYRDLPRLLSVAETGQQVANVLLSVDETYADILPVHTSASS-KSAFVSIMRGCDNMCTYCIVPFTRGRERSRPLQSILEEVRMLSSQGVKEVTLLGQNVNSYRDTSSAQ----FPNAESPPLSRGFSTIYKHKKGGLRFVDLLDKVSQIDPEMRIRFTSPHPKDFPDEVLQLIAERHNICKQIHIPAQSGSNKVLETMRRGYSREVYLDLIQNIQESIPGVSLSSDFITGFCEETEEDHLQTLSLLRTVSYNAAFLFAYSMRKKTRAYHRLQDDVPQEVKQRRLAELIAVFREEAGRVNQRAVGSTQLVLVEGPSKRSPEELCGRNDGNTKVIFSEAPKVGGLAQENSLQNR--IQSGDYVLVKITSSSSQSLKG 574
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Xenopus
Match: cwf19l1 (CWF19 like cell cycle control factor 1 [Source:Xenbase;Acc:XB-GENE-995269])

HSP 1 Score: 121.709 bits (304), Expect = 9.439e-29
Identity = 126/510 (24.71%), Postives = 213/510 (41.76%), Query Frame = 2 
Query:   80 SNFSNDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVAL---------SLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSY-RDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKE---IRIRFTSPHPKDFPDEVLHL-----IRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSIAT 1555
            S     + ++I T+GC  N +D E     L + GY+ T   ++A + LL +C ++  AED   + +K     NK     K+ + GC+ +    +     ++ + +       + + R+++ ++   KG +V L          L     D+  +R   K      +SI  GC N CTYC     RG+  S  ++ ++        +G+ EI L  ++  +Y RD+                              G     LL  + +V  E   +R+  T+P     P  + HL     I   P +   LH+P QS S +VL +M R Y    +  +VD ++  +P + + +D I GF GET+ED + TL L++  ++  +F   +  R  T A    ++ V   +K  R  EL+  F   +   ++  IG  Q VL+   S   S  +V  N    +V+ PK             +   +G  V VKI  A    +KG P+  S I T
Sbjct:   54 STIPGTQKIWIRTWGCSHNNSDGEYMAGQLAAYGYSITEQPEQADLWLLNSCTVKSPAEDHFRNSIKKAQEANK-----KVVLSGCVPQAQPRQEYMKGLSIIGV-------QQIDRVVEVVEETIKGHSVRLLGQKKDNGKRLGGARLDLPKIR---KNPLIEIISINTGCLNACTYCKTKHARGELASYPVEELVDRAAQSFQEGVCEIWLTSEDTGAYGRDI------------------------------GTDLPTLLWKLVEVIPEGAMLRLGMTNP-----PYILEHLEEMAKILNHPRVYAFLHIPVQSASDSVLMDMKREYCIADFKRVVDFLKERVPGITIATDIICGFPGETDEDFKETLKLVEEYKFPSLFINQFYPRPGTPAAK--MEQVPAHVKKQRTKELSQLFHSYSPYDHK--IGEEQHVLVTEES-FDSQYYVSHNRFYEQVLVPK-------------DPAFVGKMVEVKIFEAGKHFMKGQPVQDSYIYT 495
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Xenopus
Match: cwf19l1 (CWF19 like cell cycle control factor 1 [Source:Xenbase;Acc:XB-GENE-995269])

HSP 1 Score: 121.324 bits (303), Expect = 1.065e-28
Identity = 126/510 (24.71%), Postives = 213/510 (41.76%), Query Frame = 2 
Query:   80 SNFSNDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVAL---------SLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSY-RDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKE---IRIRFTSPHPKDFPDEVLHL-----IRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSIAT 1555
            S     + ++I T+GC  N +D E     L + GY+ T   ++A + LL +C ++  AED   + +K     NK     K+ + GC+ +    +     ++ + +       + + R+++ ++   KG +V L          L     D+  +R   K      +SI  GC N CTYC     RG+  S  ++ ++        +G+ EI L  ++  +Y RD+                              G     LL  + +V  E   +R+  T+P     P  + HL     I   P +   LH+P QS S +VL +M R Y    +  +VD ++  +P + + +D I GF GET+ED + TL L++  ++  +F   +  R  T A    ++ V   +K  R  EL+  F   +   ++  IG  Q VL+   S   S  +V  N    +V+ PK             +   +G  V VKI  A    +KG P+  S I T
Sbjct:   54 STIPGTQKIWIRTWGCSHNNSDGEYMAGQLAAYGYSITEQPEQADLWLLNSCTVKSPAEDHFRNSIKKAQEANK-----KVVLSGCVPQAQPRQEYMKGLSIIGV-------QQIDRVVEVVEETIKGHSVRLLGQKKDNGKRLGGARLDLPKIR---KNPLIEIISINTGCLNACTYCKTKHARGELASYPVEELVDRAAQSFQEGVCEIWLTSEDTGAYGRDI------------------------------GTDLPTLLWKLVEVIPEGAMLRLGMTNP-----PYILEHLEEMAKILNHPRVYAFLHIPVQSASDSVLMDMKREYCIADFKRVVDFLKERVPGITIATDIICGFPGETDEDFKETLKLVEEYKFPSLFINQFYPRPGTPAAK--MEQVPAHVKKQRTKELSQLFHSYSPYDHK--IGEEQHVLVTEES-FDSQYYVSHNRFYEQVLVPK-------------DPAFVGKMVEVKIFEAGKHFMKGQPVQDSYIYT 495
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Xenopus
Match: cwf19l1 (CWF19 like cell cycle control factor 1 [Source:Xenbase;Acc:XB-GENE-995269])

HSP 1 Score: 120.939 bits (302), Expect = 1.438e-28
Identity = 126/510 (24.71%), Postives = 213/510 (41.76%), Query Frame = 2 
Query:   80 SNFSNDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVAL---------SLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSY-RDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKE---IRIRFTSPHPKDFPDEVLHL-----IRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSIAT 1555
            S     + ++I T+GC  N +D E     L + GY+ T   ++A + LL +C ++  AED   + +K     NK     K+ + GC+ +    +     ++ + +       + + R+++ ++   KG +V L          L     D+  +R   K      +SI  GC N CTYC     RG+  S  ++ ++        +G+ EI L  ++  +Y RD+                              G     LL  + +V  E   +R+  T+P     P  + HL     I   P +   LH+P QS S +VL +M R Y    +  +VD ++  +P + + +D I GF GET+ED + TL L++  ++  +F   +  R  T A    ++ V   +K  R  EL+  F   +   ++  IG  Q VL+   S   S  +V  N    +V+ PK             +   +G  V VKI  A    +KG P+  S I T
Sbjct:   54 STIPGTQKIWIRTWGCSHNNSDGEYMAGQLAAYGYSITEQPEQADLWLLNSCTVKSPAEDHFRNSIKKAQEANK-----KVVLSGCVPQAQPRQEYMKGLSIIGV-------QQIDRVVEVVEETIKGHSVRLLGQKKDNGKRLGGARLDLPKIR---KNPLIEIISINTGCLNACTYCKTKHARGELASYPVEELVDRAAQSFQEGVCEIWLTSEDTGAYGRDI------------------------------GTDLPTLLWKLVEVIPEGAMLRLGMTNP-----PYILEHLEEMAKILNHPRVYAFLHIPVQSASDSVLMDMKREYCIADFKRVVDFLKERVPGITIATDIICGFPGETDEDFKETLKLVEEYKFPSLFINQFYPRPGTPAAK--MEQVPAHVKKQRTKELSQLFHSYSPYDHK--IGEEQHVLVTEES-FDSQYYVSHNRFYEQVLVPK-------------DPAFVGKMVEVKIFEAGKHFMKGQPVQDSYIYT 495
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Mouse
Match: Cdk5rap1 (CDK5 regulatory subunit associated protein 1 [Source:MGI Symbol;Acc:MGI:1914221])

HSP 1 Score: 561.992 bits (1447), Expect = 0.000e+0
Identity = 286/495 (57.78%), Postives = 362/495 (73.13%), Query Frame = 2 
Query:   86 FSNDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSK----LKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEI---PQIGDYVAVKITAATSQTLKGDPICRSSI 1549
                + VY+ETYGCQMN ND+EIA SIL+  GY  T+NL+EA VILL+TC+IRE AE  IW+RL     +  +  +    L+IG+LGCMAERLK ++L N    V+L+ GPDAYRDLPRLL  +++G++  NV LSL+ETYADI PV+ S     SAFVSIMRGCDNMC+YCIVPFTRG+ERSR + SIL EV+ LS+QG+KE+ LLGQNVNS+RD SE        +     L+ GF+T YKPK GG RF  LLD VS+++ E+RIRFTSPHPKDFPDEVL LIR   NICKQ+HLPAQSGSS VL  M RGY+REAY+ LV  VR  IP V+L+SDFI+GFCGETE+DH+ T+SLL+ V+YN  F + YSMR+KT+A+HRL DDV +E+K  R  EL   FR+   + N++ +G  QLVL++G SKRS+ +  GRN+AN KVIFP   +    + ITN  +    Q GDYV VKIT+A+SQTLKG  +CR+++
Sbjct:   95 LGRQRKVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQEADVILLVTCSIREKAEQTIWNRLHQLKVLKTKRPRSRVPLRIGILGCMAERLKGEIL-NREKMVDLLAGPDAYRDLPRLLAVVESGQQAANVLLSLDETYADIMPVQTSPSAT-SAFVSIMRGCDNMCSYCIVPFTRGRERSRPVASILDEVRKLSEQGLKEVTLLGQNVNSFRDNSEVQ----FNNAGSANLSRGFTTNYKPKQGGLRFSHLLDQVSRIDPEMRIRFTSPHPKDFPDEVLQLIRERHNICKQIHLPAQSGSSRVLDAMRRGYSREAYVALVHHVRETIPGVSLSSDFITGFCGETEDDHRQTVSLLREVQYNTGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITVFREEASKANKTSVGCSQLVLVEGFSKRSTTDLCGRNDANLKVIFPDAEV----EDITNPGLKVRAQPGDYVLVKITSASSQTLKGHILCRTTM 579
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Mouse
Match: Cdk5rap1 (CDK5 regulatory subunit associated protein 1 [Source:MGI Symbol;Acc:MGI:1914221])

HSP 1 Score: 561.992 bits (1447), Expect = 0.000e+0
Identity = 286/495 (57.78%), Postives = 362/495 (73.13%), Query Frame = 2 
Query:   86 FSNDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSK----LKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEI---PQIGDYVAVKITAATSQTLKGDPICRSSI 1549
                + VY+ETYGCQMN ND+EIA SIL+  GY  T+NL+EA VILL+TC+IRE AE  IW+RL     +  +  +    L+IG+LGCMAERLK ++L N    V+L+ GPDAYRDLPRLL  +++G++  NV LSL+ETYADI PV+ S     SAFVSIMRGCDNMC+YCIVPFTRG+ERSR + SIL EV+ LS+QG+KE+ LLGQNVNS+RD SE        +     L+ GF+T YKPK GG RF  LLD VS+++ E+RIRFTSPHPKDFPDEVL LIR   NICKQ+HLPAQSGSS VL  M RGY+REAY+ LV  VR  IP V+L+SDFI+GFCGETE+DH+ T+SLL+ V+YN  F + YSMR+KT+A+HRL DDV +E+K  R  EL   FR+   + N++ +G  QLVL++G SKRS+ +  GRN+AN KVIFP   +    + ITN  +    Q GDYV VKIT+A+SQTLKG  +CR+++
Sbjct:   95 LGRQRKVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQEADVILLVTCSIREKAEQTIWNRLHQLKVLKTKRPRSRVPLRIGILGCMAERLKGEIL-NREKMVDLLAGPDAYRDLPRLLAVVESGQQAANVLLSLDETYADIMPVQTSPSAT-SAFVSIMRGCDNMCSYCIVPFTRGRERSRPVASILDEVRKLSEQGLKEVTLLGQNVNSFRDNSEVQ----FNNAGSANLSRGFTTNYKPKQGGLRFSHLLDQVSRIDPEMRIRFTSPHPKDFPDEVLQLIRERHNICKQIHLPAQSGSSRVLDAMRRGYSREAYVALVHHVRETIPGVSLSSDFITGFCGETEDDHRQTVSLLREVQYNTGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITVFREEASKANKTSVGCSQLVLVEGFSKRSTTDLCGRNDANLKVIFPDAEV----EDITNPGLKVRAQPGDYVLVKITSASSQTLKGHILCRTTM 579
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Mouse
Match: Cdkal1 (CDK5 regulatory subunit associated protein 1-like 1 [Source:MGI Symbol;Acc:MGI:1921765])

HSP 1 Score: 119.783 bits (299), Expect = 2.613e-28
Identity = 128/523 (24.47%), Postives = 217/523 (41.49%), Query Frame = 2 
Query:   41 KKYLHYSNIQRLCSNFSNDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVAL---------SLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSY-RDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKE---IRIRFTSPHPKDFPDEVLHL-----IRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSIAT 1555
            +KYL         S     + ++I T+GC  N +D E     L + GY  T N  +A + LL +C ++  AED   + +K     NK     K+ + GC+ +   ++  ++ +  ++++      + + R+++ ++   KG +V L          L     D+  +R   K      +SI  GC N CTYC     RG   S  ID +++  +    +G+ EI L  ++  +Y RD+                              G     LL  + +V  E   +R+  T+P     P  + HL     I   P +   LH+P QS S +VL +M R Y    +  +VD ++  +P + + +D I GF GET++D Q T+ L++  ++  +F   +  R  T A     + V   +K  R  +L+  F   +       IG  Q VL+   S   S  +V  N    +V+ PK           N     +G  V V I  +    LKG P+  + + T
Sbjct:   44 QKYLQEEPRPPSDSTIPGIQKIWIRTWGCSHNNSDGEYMAGQLAAYGYKITENASDADLWLLNSCTVKNPAEDHFRNSIKKAQEENK-----KVVLAGCVPQ---AQPRQDYLKGLSII----GVQQIDRVVEVVEETIKGHSVRLLGQKKDNGKRLGGARLDLPKIR---KNPLIEIISINTGCLNACTYCKTKHARGNLASYPIDELVERAKQSFQEGVCEIWLTSEDTGAYGRDI------------------------------GTDLPTLLWKLVEVIPEGAMLRLGMTNP-----PYILEHLEEMAKILNHPRVYAFLHIPVQSASDSVLMDMKREYCVADFKRVVDFLKEKVPGITIATDIICGFPGETDQDFQETVKLVEEYKFPSLFINQFYPRPGTPAAK--AEQVPAHVKKQRTKDLSRVFH--SYNPYDHKIGERQQVLVTEES-FDSKFYVAHNRFYEQVLVPK-----------NPAF--MGKMVEVDIYESGKHFLKGQPVSETRVYT 498
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Mouse
Match: Cdkal1 (CDK5 regulatory subunit associated protein 1-like 1 [Source:MGI Symbol;Acc:MGI:1921765])

HSP 1 Score: 119.783 bits (299), Expect = 3.501e-28
Identity = 128/523 (24.47%), Postives = 217/523 (41.49%), Query Frame = 2 
Query:   41 KKYLHYSNIQRLCSNFSNDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVAL---------SLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSY-RDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKE---IRIRFTSPHPKDFPDEVLHL-----IRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSIAT 1555
            +KYL         S     + ++I T+GC  N +D E     L + GY  T N  +A + LL +C ++  AED   + +K     NK     K+ + GC+ +   ++  ++ +  ++++      + + R+++ ++   KG +V L          L     D+  +R   K      +SI  GC N CTYC     RG   S  ID +++  +    +G+ EI L  ++  +Y RD+                              G     LL  + +V  E   +R+  T+P     P  + HL     I   P +   LH+P QS S +VL +M R Y    +  +VD ++  +P + + +D I GF GET++D Q T+ L++  ++  +F   +  R  T A     + V   +K  R  +L+  F   +       IG  Q VL+   S   S  +V  N    +V+ PK           N     +G  V V I  +    LKG P+  + + T
Sbjct:   44 QKYLQEEPRPPSDSTIPGIQKIWIRTWGCSHNNSDGEYMAGQLAAYGYKITENASDADLWLLNSCTVKNPAEDHFRNSIKKAQEENK-----KVVLAGCVPQ---AQPRQDYLKGLSII----GVQQIDRVVEVVEETIKGHSVRLLGQKKDNGKRLGGARLDLPKIR---KNPLIEIISINTGCLNACTYCKTKHARGNLASYPIDELVERAKQSFQEGVCEIWLTSEDTGAYGRDI------------------------------GTDLPTLLWKLVEVIPEGAMLRLGMTNP-----PYILEHLEEMAKILNHPRVYAFLHIPVQSASDSVLMDMKREYCVADFKRVVDFLKEKVPGITIATDIICGFPGETDQDFQETVKLVEEYKFPSLFINQFYPRPGTPAAK--AEQVPAHVKKQRTKDLSRVFH--SYNPYDHKIGERQQVLVTEES-FDSKFYVAHNRFYEQVLVPK-----------NPAF--MGKMVEVDIYESGKHFLKGQPVSETRVYT 498
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Mouse
Match: Cdk5rap1 (CDK5 regulatory subunit associated protein 1 [Source:MGI Symbol;Acc:MGI:1914221])

HSP 1 Score: 97.8265 bits (242), Expect = 4.441e-23
Identity = 45/87 (51.72%), Postives = 59/87 (67.82%), Query Frame = 2 
Query:   86 FSNDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSK----LKIGVLG 334
                + VY+ETYGCQMN ND+EIA SIL+  GY  T+NL+EA VILL+TC+IRE AE  IW+RL     +  +  +    L+IG+LG
Sbjct:   95 LGRQRKVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQEADVILLVTCSIREKAEQTIWNRLHQLKVLKTKRPRSRVPLRIGILG 181
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. UniProt/SwissProt
Match: sp|Q8BTW8|CK5P1_MOUSE (Mitochondrial tRNA methylthiotransferase CDK5RAP1 OS=Mus musculus OX=10090 GN=Cdk5rap1 PE=1 SV=2)

HSP 1 Score: 561.992 bits (1447), Expect = 0.000e+0
Identity = 286/495 (57.78%), Postives = 362/495 (73.13%), Query Frame = 2 
Query:   86 FSNDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSK----LKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEI---PQIGDYVAVKITAATSQTLKGDPICRSSI 1549
                + VY+ETYGCQMN ND+EIA SIL+  GY  T+NL+EA VILL+TC+IRE AE  IW+RL     +  +  +    L+IG+LGCMAERLK ++L N    V+L+ GPDAYRDLPRLL  +++G++  NV LSL+ETYADI PV+ S     SAFVSIMRGCDNMC+YCIVPFTRG+ERSR + SIL EV+ LS+QG+KE+ LLGQNVNS+RD SE        +     L+ GF+T YKPK GG RF  LLD VS+++ E+RIRFTSPHPKDFPDEVL LIR   NICKQ+HLPAQSGSS VL  M RGY+REAY+ LV  VR  IP V+L+SDFI+GFCGETE+DH+ T+SLL+ V+YN  F + YSMR+KT+A+HRL DDV +E+K  R  EL   FR+   + N++ +G  QLVL++G SKRS+ +  GRN+AN KVIFP   +    + ITN  +    Q GDYV VKIT+A+SQTLKG  +CR+++
Sbjct:   95 LGRQRKVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQEADVILLVTCSIREKAEQTIWNRLHQLKVLKTKRPRSRVPLRIGILGCMAERLKGEIL-NREKMVDLLAGPDAYRDLPRLLAVVESGQQAANVLLSLDETYADIMPVQTSPSAT-SAFVSIMRGCDNMCSYCIVPFTRGRERSRPVASILDEVRKLSEQGLKEVTLLGQNVNSFRDNSEVQ----FNNAGSANLSRGFTTNYKPKQGGLRFSHLLDQVSRIDPEMRIRFTSPHPKDFPDEVLQLIRERHNICKQIHLPAQSGSSRVLDAMRRGYSREAYVALVHHVRETIPGVSLSSDFITGFCGETEDDHRQTVSLLREVQYNTGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITVFREEASKANKTSVGCSQLVLVEGFSKRSTTDLCGRNDANLKVIFPDAEV----EDITNPGLKVRAQPGDYVLVKITSASSQTLKGHILCRTTM 579
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. UniProt/SwissProt
Match: sp|Q9JLH6|CK5P1_RAT (Mitochondrial tRNA methylthiotransferase CDK5RAP1 OS=Rattus norvegicus OX=10116 GN=Cdk5rap1 PE=1 SV=1)

HSP 1 Score: 560.451 bits (1443), Expect = 0.000e+0
Identity = 284/495 (57.37%), Postives = 360/495 (72.73%), Query Frame = 2 
Query:   86 FSNDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSK----LKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEI---PQIGDYVAVKITAATSQTLKGDPICRSSI 1549
                + VY+ETYGCQMN ND+EIA SIL+  GY  T+NL+EA VILL+TC+IRE AE  IW+RL     +  +  +    L+IG+LGCMAERLK ++L N    V+L+ GPDAYRDLPRLL  +++G++  NV LSL+ETYADI PV+ S     SAFVSIMRGCDNMC+YCIVPFTRG+ERSR + SIL EV+ LS+QG+KE+ LLGQNVNS+RD SE        S     L+ GF+T YKPK GG RF  LLD VS+++ E+RIRFTSPHPKDFPDEVL LIR   NICKQ+HLPAQSGSS VL+ M RGY+REAY+ LV  +R  IP V L+SDFI+GFCGETE+DH  T+SLL+ V+YN  F + YSMR+KT+A+HRL DDV +E+K  R  EL   FR+   ++N + +G  QLVL++G SKRS+ +  GRN+AN KVIFP   +    + IT+  +    Q GDYV VKI +A+SQTLKG  +CR+++
Sbjct:   93 LGRQRKVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQEADVILLVTCSIREKAEQTIWNRLHQLKVLKAKRPRSRVPLRIGILGCMAERLKGEIL-NREKMVDLLAGPDAYRDLPRLLAVVESGQQAANVLLSLDETYADIMPVQTSPSAT-SAFVSIMRGCDNMCSYCIVPFTRGRERSRPVASILDEVRKLSEQGLKEVTLLGQNVNSFRDNSEVQ----FSSTGSANLSRGFTTNYKPKQGGLRFSHLLDQVSRIDPEMRIRFTSPHPKDFPDEVLQLIRERHNICKQIHLPAQSGSSRVLEAMRRGYSREAYVALVHHIREAIPGVGLSSDFITGFCGETEDDHLQTVSLLREVQYNTGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITVFREEASKVNATSVGCTQLVLVEGFSKRSTTDLCGRNDANLKVIFPDAEV----EDITDPGLKVRAQPGDYVLVKIISASSQTLKGHILCRTTM 577
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. UniProt/SwissProt
Match: sp|Q96SZ6|CK5P1_HUMAN (Mitochondrial tRNA methylthiotransferase CDK5RAP1 OS=Homo sapiens OX=9606 GN=CDK5RAP1 PE=1 SV=2)

HSP 1 Score: 554.288 bits (1427), Expect = 0.000e+0
Identity = 285/507 (56.21%), Postives = 359/507 (70.81%), Query Frame = 2 
Query:   86 FSNDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSK----LKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQ--------------GIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLP-FNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSI 1549
                + VY+ETYGCQMN ND+EIA SIL+  GY  T+NL+EA VILL+TC+IRE AE  IW+RL     +  R  +    L+IG+LGCMAERLK ++L N    V+++ GPDAYRDLPRLL   ++G++  NV LSL+ETYAD+ PV+ S+    SAFVSIMRGCDNMC+YCIVPFTRG+ERSR I SIL+EV+ LS+Q              G+KE+ LLGQNVNS+RD SE      V     T L+ GF+T YK K GG RF  LLD VS+V+ E+RIRFTSPHPKDFPDEVL LI    NICKQ+HLPAQSGSS VL+ M RGY+REAY+ LV  +R  IP V+L+SDFI+GFCGETEEDH  T+SLL+ V+YN  F + YSMR+KT+A+HRL DDV +E+K  R  EL   FR+   + NQ+ +G  QLVL++G SKRS+ +  GRN+ N KVIFP   +   NN  +     P  GDYV VKIT+A+SQTL+G  +CR+++
Sbjct:   96 LGRQRKVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQEADVILLVTCSIREKAEQTIWNRLHQLKALKTRRPRSRVPLRIGILGCMAERLKEEIL-NREKMVDILAGPDAYRDLPRLLAVAESGQQAANVLLSLDETYADVMPVQTSASAT-SAFVSIMRGCDNMCSYCIVPFTRGRERSRPIASILEEVKKLSEQVFLPPRPPKVLGLQGLKEVTLLGQNVNSFRDNSEVQFNSAV----PTNLSRGFTTNYKTKQGGLRFAHLLDQVSRVDPEMRIRFTSPHPKDFPDEVLQLIHERDNICKQIHLPAQSGSSRVLEAMRRGYSREAYVELVHHIRESIPGVSLSSDFIAGFCGETEEDHVQTVSLLREVQYNMGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITIFREEATKANQTSVGCTQLVLVEGLSKRSATDLCGRNDGNLKVIFPDAEMEDVNNPGLRVRAQP--GDYVLVKITSASSQTLRGHVLCRTTL 594
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BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. UniProt/SwissProt
Match: sp|Q9VGZ1|CK5P1_DROME (CDK5RAP1-like protein OS=Drosophila melanogaster OX=7227 GN=CG6345 PE=2 SV=1)

HSP 1 Score: 533.487 bits (1373), Expect = 0.000e+0
Identity = 267/496 (53.83%), Postives = 350/496 (70.56%), Query Frame = 2 
Query:   92 NDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGI-NKRSSK---LKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKA-GRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSIATFNS 1564
            N + V+ E YGCQMN ND+E+  SILK +GY      +EA VI+L+TCA+R+GAE +I +RLK+   + NKRS++   L++ +LGCMAERLK KLLE     V+++ GPD+Y+DLPRLL   +  G   +NV LSL+ETYAD+ PVRL+S+  P+AFVSIMRGCDNMCTYCIVPFTRG+ERSR + SI+ EV+ L++QG+KE+ LLGQNVNSYRD +   E   +    K     GFSTVYKPKTGG  F  LL +V+Q   ++RIRFTSPHPKDF DEVL +IR  PN+CKQLHLPAQSG++ VL+ M RGY+REAYL LV  +R  +PNV L+SDFI GFCGETEE+ Q T+SL++ V+YN  + + YSMREKT A  R  DDV   +K+ R   +   FR+   ++++   G  QL+LI+G SKRS   W GRN+AN KVI P + +P + D         +GD++AV+I  + SQ LKG P+  S+I  F+S
Sbjct:   90 NGRKVHFEVYGCQMNTNDTEVVFSILKENGYLRCQEPEEADVIMLVTCAVRDGAEQRIRNRLKHLRAMKNKRSTRRHPLQLTLLGCMAERLKEKLLEQEQC-VDVIAGPDSYKDLPRLLAISRHYGNSAINVLLSLDETYADVMPVRLNSES-PTAFVSIMRGCDNMCTYCIVPFTRGRERSRPLASIVAEVKALAEQGVKEVTLLGQNVNSYRDRTAQEEQDSL----KATPVPGFSTVYKPKTGGTPFAALLRSVAQAVPDMRIRFTSPHPKDFSDEVLEVIRDHPNVCKQLHLPAQSGNTQVLERMRRGYSREAYLELVQHIRQFLPNVGLSSDFICGFCGETEEEFQDTVSLIQQVQYNVAYLFAYSMREKTTAHRRYKDDVPINVKNERLQRMVQVFREGATQLHRKMEGQEQLILIEGKSKRSDAHWFGRNDANIKVIVPSIYVPISGDSTARKSF-GVGDFLAVRIEESNSQVLKGTPLELSNITDFHS 578
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BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. UniProt/SwissProt
Match: sp|Q2R1U4|CK5P1_ORYSJ (CDK5RAP1-like protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g0592800 PE=2 SV=1)

HSP 1 Score: 520.39 bits (1339), Expect = 7.925e-179
Identity = 261/501 (52.10%), Postives = 354/501 (70.66%), Query Frame = 2 
Query:  104 VYIETYGCQMNFNDSEIAMSILKSDGYTH-TTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINK---------RSSKL---KIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRS-SNEWVGRNEANTKVIFPKLSLP--FNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSIATF 1558
            +Y ETYGCQMN ND EI +SI+K++GY     + + A +I + TCAIR+ AE K+W RL Y+  + +         RS  L   KI VLGCMAERLK K+L++    V++VCGPDAYRDLPRLL+E+  G+KG+N  LSLEETYADITPVR+S   + +AFVSIMRGC+NMC++CIVPFTRG+ERSR + SI++EV  L   G+KE++LLGQNVNSY D SE  E+      +  +L++GFS++ K K  G RF DLLD +S    E+R RFTSPHPKD+PDE+L+L+R   N+CK +H+PAQSGSS VL+ M RGYTREAYL LV K+R IIP+V L+SDFISGFCGETEE+H  TL+L++ V Y+  + + YSMREKT A    +DDV D++K  R  EL + FR+ T +I  S +G++QLVL++G +KR+   E +G+ +   +V F  + +P  F  D +     P +GD++ VKIT +++ +L GD I R+S++ F
Sbjct:   95 IYHETYGCQMNVNDMEIVLSIMKNEGYDEIVPDPESAEIIFINTCAIRDNAEQKVWQRLNYFWFLKRQWKANVAAGRSRSLRPPKIAVLGCMAERLKEKILDSDKM-VDVVCGPDAYRDLPRLLQEVDYGQKGINTLLSLEETYADITPVRISDNSV-TAFVSIMRGCNNMCSFCIVPFTRGRERSRPVSSIVREVGELWKAGVKEVMLLGQNVNSYNDTSEVEELE---PGKNWELSEGFSSMCKVKNMGLRFADLLDQLSLEYPEMRFRFTSPHPKDYPDELLYLMRDRHNVCKLIHMPAQSGSSAVLERMRRGYTREAYLELVQKIRSIIPDVGLSSDFISGFCGETEEEHAETLTLVRAVGYDMAYMFAYSMREKTHAHRNYVDDVPDDVKQRRLAELISTFRETTAKIYDSQVGTVQLVLVEGPNKRAPETEMIGKTDRGHRVSFASVPVPHTFEGDELRK---PVVGDFIEVKITKSSTASLSGDVIARTSLSKF 587
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BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. TrEMBL
Match: A0A3S1BF20 (Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_010003 PE=4 SV=1)

HSP 1 Score: 603.979 bits (1556), Expect = 0.000e+0
Identity = 293/493 (59.43%), Postives = 370/493 (75.05%), Query Frame = 2 
Query:   92 NDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSK---LKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKG-MNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSIATF 1558
            N + VYIETYGCQMN ND+EI  SILK  G+ ++T++++A V+L MTCAIREGAEDKIW R+ Y+  + KR  K   LKIG+LGCMAERLK K+++     V++VCGPDAY+DLPRLL    +  +  +NV LSLEETYAD+ PVRL+S   PSAFVSIMRGCDNMCTYCIVPFTRG+ERSR + SI+ E+  LS+QG KE+ LLGQNVNSYRD+S    +        T+L+ GF TVYKPKTGGRRF +LL+AV+ VN E+RIRFTSPHPKDFPDEVL  I A PNICKQ+HLPAQSG+S+VL+ MGRGYTREAYL LV+ VR  IP VAL+SDFI+GFCGETEE H+ TLSL+  V+YNF +C+PYSMR+KT+A+HRL D+V  E+K  RH EL A FR+     + + +G   L+L++G SKRSS +  GRN+ANTK +FP   +P+ ++        + GDYV V++T+ATS   KG  + R+S+  F
Sbjct:   91 NSRSVYIETYGCQMNVNDTEIVRSILKQSGFVNSTSIEKADVVLAMTCAIREGAEDKIWRRMHYFKALKKRRGKRHPLKIGLLGCMAERLKHKIIDQEQM-VDVVCGPDAYKDLPRLLVVTSSSNQAAVNVQLSLEETYADVVPVRLNSDS-PSAFVSIMRGCDNMCTYCIVPFTRGRERSRPLTSIVNEINILSEQGCKEVTLLGQNVNSYRDLSSEEHI-----SSPTQLSAGFKTVYKPKTGGRRFAELLEAVALVNPEMRIRFTSPHPKDFPDEVLGAITAYPNICKQIHLPAQSGNSSVLEAMGRGYTREAYLELVEHVRKAIPGVALSSDFIAGFCGETEEAHKDTLSLMDTVKYNFAYCFPYSMRQKTRAYHRLEDNVPKEVKMKRHEELHATFRKHAEVRHMAQVGEKHLILVEGESKRSSLDLAGRNDANTKAVFPNEPIPYGSENGVEKISAKPGDYVVVELTSATSLGFKGKALYRTSLQEF 576
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BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. TrEMBL
Match: A0A1S3JD81 (CDK5 regulatory subunit-associated protein 1 OS=Lingula unguis OX=7574 GN=LOC106172086 PE=4 SV=1)

HSP 1 Score: 602.438 bits (1552), Expect = 0.000e+0
Identity = 293/496 (59.07%), Postives = 373/496 (75.20%), Query Frame = 2 
Query:   98 KMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSKL------KIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIP-QIGDYVAVKITAATSQTLKGDPICRSSIATFNS 1564
            + V IET+GCQMNF+D+E+A SILK  G+  T  L EA V+L++TCAIREGAE KI  RL YY G+ KR  KL      KIG+LGCMAERLK+KLLE     V+++CGPD+YRDLPR+L   +AG   +NV LS+EETYADI PVRL+     SAFVSIMRGCDNMC+YCIVPFTRG+ERSR I+SI+ E++TLSDQG+KE++LLGQNVNSYRD+SES    G+     T +++GF TVY+PK GG+RF DLLD  S V+ ++RIRFTSPHPKDFPDEVL+LI+  PNICKQ+HLPAQSG+S VL  M RGYTREAY+ L+  ++ ++P V+LTSDFI+GFCGETEE H+ TLSLL+  +Y+F++ +PYSMR+KTKAFH L DDV   +K  R  E+    R+      +S IG +QLVLIDG SK+SS + VGRN+   KV+FP+ SLP N+D    S  P + GDYV VK+T+A  QT++G P+ R+S+  F +
Sbjct:  110 RKVCIETHGCQMNFSDTEVAWSILKEKGFERTDCLPEADVLLVVTCAIREGAEQKIRKRLAYYSGLKKRRQKLNPKVPVKIGILGCMAERLKTKLLEQ--EDVDIICGPDSYRDLPRMLASSQAGHSAVNVQLSMEETYADIMPVRLNENS-KSAFVSIMRGCDNMCSYCIVPFTRGRERSRPIESIINEIKTLSDQGVKEVMLLGQNVNSYRDLSESQHFGGIGGDRPTVMSEGFETVYRPKHGGKRFSDLLDRASLVDPDMRIRFTSPHPKDFPDEVLYLIQDRPNICKQIHLPAQSGNSDVLAAMRRGYTREAYIQLLHHIQEVLPGVSLTSDFIAGFCGETEEAHEDTLSLLRLAKYDFVWAFPYSMRQKTKAFHWLQDDVPQIVKQRRMEEILDTARKEMGMKRKSEIGKLQLVLIDGDSKKSSADLVGRNDGGIKVVFPQCSLPGNSDQDAASSSPLKPGDYVIVKVTSAEGQTMRGVPVARNSLREFTT 602
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BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. TrEMBL
Match: A0A210QJX1 (CDK5 regulatory subunit-associated protein 1 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT07024 PE=4 SV=1)

HSP 1 Score: 599.742 bits (1545), Expect = 0.000e+0
Identity = 297/498 (59.64%), Postives = 367/498 (73.69%), Query Frame = 2 
Query:   98 KMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSIATF-NSEIINSGNN 1588
            + VY ETYGCQMN ND+EIAM+IL+ +G+  TTN++ A V+ +MTC+IREGAE KIW RL+YY  + K+ S +KIG+LGCMAE LK K++E     V++VCGPDAY+DLPRLL     G+  +NV LS +ETYAD+ PVR++ K  PSAFVSIMRGCDNMCTYCIVPFTRG+ERSR I SI++E+QTLSDQG+KEI LLGQNVNSY D SES    G+ +   TK + GF TVYKPK GGRRF DLLDAVS+V+  IRIRFTSPHPKDFPDEVL+LIR   N+C  +HLPAQSGS++VLQ M RGYTRE+YL LV  +  IIP+V+L+SDFI+GFCGETE DH  TLSL++ V YNF + +PYSMR KT A H+L DDV D++K  RHLE+A  FR+  L  NQ+ IG  QLVL++  SKRS+++  GRN+ NTKVI     L  +     N    + GDYV  +I + +SQ L G P+   SI  F  SE    GNN
Sbjct:   99 RKVYFETYGCQMNVNDTEIAMAILEKNGFEMTTNVQAADVLFMMTCSIREGAEQKIWKRLEYYKSLKKKRSSMKIGILGCMAENLKKKIIEKD-KLVDIVCGPDAYKDLPRLLDVTSTGQAAVNVMLSFDETYADVMPVRVN-KDSPSAFVSIMRGCDNMCTYCIVPFTRGRERSRPIASIVEEIQTLSDQGLKEITLLGQNVNSYLDKSESKFYGGLSADAHTKNSSGFKTVYKPKVGGRRFSDLLDAVSRVDPSIRIRFTSPHPKDFPDEVLYLIRDRANVCNHIHLPAQSGSTSVLQAMRRGYTRESYLELVHHIWDIIPDVSLSSDFIAGFCGETEADHVDTLSLMEIVHYNFAYMFPYSMRNKTHAHHKLKDDVPDDVKKRRHLEIAKTFRKGALMRNQAQIGQKQLVLVESVSKRSTDDMAGRNDGNTKVI-----LVGSGTTAVNGLNLKPGDYVVAEIASCSSQVLHGLPLYTCSIQDFYRSEHSCQGNN 589
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. TrEMBL
Match: R7TUC4 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_178593 PE=4 SV=1)

HSP 1 Score: 597.045 bits (1538), Expect = 0.000e+0
Identity = 295/498 (59.24%), Postives = 376/498 (75.50%), Query Frame = 2 
Query:   98 KMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINK-RSSK------LKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNN-DLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSIATFNSE 1567
            + V+ ETYGCQMN ND+EIA +ILK +G+    ++K++ VIL+MTC+IREGAEDKIW RLKY   + + R  K      +KIG+LGCMAERLK KL+    A V++VCGPDAYRDLPRLL     G+  +NV LSLEETYAD+ PVR++S    SAF+SIMRGCDNMCTYCIVPFTRG+ERSR IDSI++EV+ LSDQG++E+ LLGQNVNSYRD S +  V   I ++KTK + GF TVYKPK GGRRF DLL+ VS+V+ E+RIRFTSPHPKDFPDEVL+LIR   NIC  +HLPAQSGS++VL+ M RGYT E+Y+ LV+ +  IIP+VAL+SDFI+GFCGETEEDH+ TL L++ V+Y+  + +PYSMR+KT AFHRL DDV  E+K  R  ELA  +R+  L++N +  G  QL+LI+G SKRS  +  GRN+ANTKVI PK +L +N+ D       P  GDY+AVKIT A+SQ L G+P+  +++  F +E
Sbjct:  122 RKVFFETYGCQMNVNDTEIAWAILKENGFIKADDIKQSDVILVMTCSIREGAEDKIWKRLKYLKSLKQLRKQKQPSAPPVKIGILGCMAERLKEKLISTERA-VDVVCGPDAYRDLPRLLSVAGTGQSAVNVQLSLEETYADVMPVRINSTST-SAFISIMRGCDNMCTYCIVPFTRGRERSRPIDSIIEEVKILSDQGLREVTLLGQNVNSYRDTSSA--VSYSIPEDKTKNSRGFKTVYKPKVGGRRFSDLLEKVSEVDPEMRIRFTSPHPKDFPDEVLYLIRDRKNICNHIHLPAQSGSNSVLKAMRRGYTLESYMELVEHILSIIPDVALSSDFIAGFCGETEEDHEATLDLMRKVKYSVAYTFPYSMRQKTGAFHRLNDDVPSEVKLRRSGELAKVYREEVLKLNLAQTGETQLILIEGDSKRSEYDLAGRNDANTKVIIPKYTLTWNHKDGQERDARP--GDYIAVKITGASSQVLHGNPLFLTTLQNFKTE 613
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. TrEMBL
Match: A0A2C9JX73 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106071647 PE=3 SV=1)

HSP 1 Score: 595.89 bits (1535), Expect = 0.000e+0
Identity = 293/496 (59.07%), Postives = 374/496 (75.40%), Query Frame = 2 
Query:   92 NDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSK---LKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKG-MNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSIATFNSE 1567
            N++ V+IETYGCQMN ND+EI  SIL  +G+    ++ EA V+LL+TC+IREGAE+KIW +L    G+ ++  K   LK+G+LGCMAERLK+K+LE     V+++CGPDAYRDLPRLL    A  +  +NV LS+EETYAD+ PVRL+  G  SAFVSIMRGCDNMCTYCIVPFTRG+ERSR I SIL+E+ TL+DQG+K++ LLGQNVNSYRD++  +      S++ TKL+ GFSTVYKPK GG+RF  LL AV+Q+N E+RIRFTSPHPKDFPDEVL  I   PNICKQ+HLPAQSG+S VL  MGRGYTREAYL LV+ VR I+P+V L+SDFI+GFCGETEE HQ TLSL+  V+YN+ FC+PYSMREKT+A+HRL D+V  ++KS RH+EL         E+++S +G  QLVLI+G SKRSS + VGRNE NTKV+FPK          T   + Q GDYV V+I++ TS +LKG  + ++++  F+ +
Sbjct:  108 NNRQVFIETYGCQMNVNDTEIIWSILLKNGFHKALSIAEADVVLLVTCSIREGAEEKIWSKLHTLKGLKRKRGKRNPLKLGILGCMAERLKTKILEKEKM-VDIICGPDAYRDLPRLLSNTSATDEASVNVQLSMEETYADVIPVRLNP-GSVSAFVSIMRGCDNMCTYCIVPFTRGRERSRPITSILEEIHTLADQGVKDVTLLGQNVNSYRDITSGH------SEDVTKLSTGFSTVYKPKMGGQRFAALLKAVAQINPEMRIRFTSPHPKDFPDEVLETILNYPNICKQIHLPAQSGNSQVLSAMGRGYTREAYLKLVENVRKILPDVGLSSDFIAGFCGETEEAHQDTLSLMDIVKYNYAFCFPYSMREKTRAYHRLEDNVPSDVKSRRHIELYDRHLLHARELHESQVGQKQLVLIEGESKRSSEDLVGRNEVNTKVVFPK----------TPESLFQPGDYVVVEISSGTSFSLKGQVLYKTTLTDFSKQ 585
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Cavefish
Match: CDK5RAP1 (CDK5 regulatory subunit associated protein 1 [Source:NCBI gene;Acc:103033945])

HSP 1 Score: 571.237 bits (1471), Expect = 0.000e+0
Identity = 294/502 (58.57%), Postives = 370/502 (73.71%), Query Frame = 2 
Query:   89 SNDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKR----SSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIP-QIGDYVAVKITAATSQTLKGDPICRSSIATFNSEIINS 1579
            +++  VY ETYGCQMN ND+EIA SIL+  GY  T+ L EA V+LL+TC++RE AE  IW+RLK    + K+    S++LKIG+LGCMAERLK++LLE     V++V GPDAYRDLPRLL     G++  NV LSLEETYAD+ PV  S     SAFVSIMRGCDNMC++C+VPFTRG+ERSR + SIL+EV+ LSDQG+KE+ LLGQNVNSYRD+SE  +  G  S+  T L+ GFST Y+ K GG RF +LLD VS ++ ++RIRFTSPHPKDFPDEVL LI+   NICKQ+HLPAQSGS+ VLQ M RGYTREAYL LV+ +R IIP  +L+SDFI+GFCGETEEDHQ T+SLL+ V YN  F + YSMR+KT A+HRL DDV  E+K  R  EL + FR+   ++N + IGS QL+L++G SKRSS E  GRN+ N KVIFP+  LP  +    +  +P + GDYV VKIT+A SQ+L+G  I  +S+    S+ INS
Sbjct:   83 ASEPKVYFETYGCQMNVNDTEIAWSILQKGGYLRTSELSEADVVLLVTCSVREKAEQTIWNRLKQLTLLKKKRLKASAQLKIGILGCMAERLKTELLEKE-KLVDVVAGPDAYRDLPRLLSLAHGGQRASNVLLSLEETYADVMPVHNSGH---SAFVSIMRGCDNMCSFCVVPFTRGRERSRPVSSILEEVRMLSDQGVKEVTLLGQNVNSYRDLSE-EQFRG--SELSTHLSRGFSTKYRSKRGGLRFTELLDRVSLIDPDMRIRFTSPHPKDFPDEVLQLIQERKNICKQIHLPAQSGSTQVLQTMRRGYTREAYLELVENIRKIIPGASLSSDFIAGFCGETEEDHQQTMSLLREVGYNIGFLFAYSMRKKTHAYHRLQDDVPAEVKQRRLEELISVFREEAAKVNSTLIGSTQLILVEGESKRSSQELCGRNDGNLKVIFPRKDLP--SKATDSCTVPVKAGDYVLVKITSANSQSLRGQAISHTSL----SDSINS 571
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Cavefish
Match: CDK5RAP1 (CDK5 regulatory subunit associated protein 1 [Source:NCBI gene;Acc:103033945])

HSP 1 Score: 554.673 bits (1428), Expect = 0.000e+0
Identity = 287/499 (57.52%), Postives = 364/499 (72.95%), Query Frame = 2 
Query:   89 SNDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKR----SSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIP-QIGDYVAVKITAATSQTLKGDPI---CRSSIATFN 1561
            +++  VY ETYGCQMN ND+EIA SIL+  GY  T+ L EA V+LL+TC++RE AE  IW+RLK    + K+    S++LKIG+LGCMAERLK++LLE     V++V GPDAYRDLPRLL     G++  NV LSLEETYAD+ PV  S     SAFVSIMRGCDNMC++C+VPFTRG+ERSR + SIL+EV+ LSDQG+KE+ LLGQNVNSYRD+SE  +  G  S+  T L+ GFST Y+ K GG RF +LLD VS ++ ++RIRFTSPHPKDFPDEVL LI+   NICKQ+HLPAQSGS+ VLQ M RGYTREAYL LV+ +R IIP  +L+SDFI+GFCGETEEDHQ T+SLL+ V YN  F + YSMR+KT A+HRL DDV  E+K  R  EL + FR+   ++N + IGS QL+L++G SKRSS E  GRN+ N KVIFP+  LP  +    +  +P + GDYV VK+++ T    + D +   C   I++FN
Sbjct:   83 ASEPKVYFETYGCQMNVNDTEIAWSILQKGGYLRTSELSEADVVLLVTCSVREKAEQTIWNRLKQLTLLKKKRLKASAQLKIGILGCMAERLKTELLEKE-KLVDVVAGPDAYRDLPRLLSLAHGGQRASNVLLSLEETYADVMPVHNSGH---SAFVSIMRGCDNMCSFCVVPFTRGRERSRPVSSILEEVRMLSDQGVKEVTLLGQNVNSYRDLSE-EQFRG--SELSTHLSRGFSTKYRSKRGGLRFTELLDRVSLIDPDMRIRFTSPHPKDFPDEVLQLIQERKNICKQIHLPAQSGSTQVLQTMRRGYTREAYLELVENIRKIIPGASLSSDFIAGFCGETEEDHQQTMSLLREVGYNIGFLFAYSMRKKTHAYHRLQDDVPAEVKQRRLEELISVFREEAAKVNSTLIGSTQLILVEGESKRSSQELCGRNDGNLKVIFPRKDLP--SKATDSCTVPVKAGDYVLVKVSSHTVPVKRLDILLIQCCLFISSFN 572
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Cavefish
Match: cdkal1 (CDK5 regulatory subunit associated protein 1 like 1 [Source:HGNC Symbol;Acc:HGNC:21050])

HSP 1 Score: 102.064 bits (253), Expect = 1.588e-22
Identity = 120/507 (23.67%), Postives = 205/507 (40.43%), Query Frame = 2 
Query:  104 VYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVAL---------SLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSY-RDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEI------RIRFTSPHPKDFPDEVLHL-----IRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSH--IGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSIAT 1555
            V++ T+GC  N +D E     L + GY  T    +        C +      K  + L    G   +    K+ V GC+ +   ++   + +  ++++      + + R+++ +    KG +V L          L     D+  +R   K      +SI  GC N CTYC     RG   S  I+ +++  +    +G+ EI L  ++  +Y RD+                                   DL   + ++ KEI      R+  T+P     P  + HL     I   P +   LH+P QS S +VL +M R Y  + +  +VD ++  +P + + +D I GF GET+ED Q T+ L+K  R+  +F   +  R  T A    ++ V  ++K  R  EL+A F       N  H  IG  Q VL+   S   +  +V  N+   +V+ PK +  F             G  + V I  +    ++G P+  + + T
Sbjct:   61 VWVRTWGCSHNNSDGEYMAGQLAAGGYKMTGTCVDTHTHSSQPCIM------KASEYLSANVGKKAQEQNKKVVVAGCVPQ---AQPRMDYLKGLSII----GVQQIDRVVEVVDEAVKGHSVRLLGQKKDNGRRLGGARLDLPKIR---KNPLIEIISINTGCLNACTYCKTKHARGDLASYPIEELVERARQSFQEGVCEIWLTSEDTGAYGRDIG---------------------------------TDLPSLLWELVKEIPEGCMLRLGMTNP-----PYILEHLEEMAKILNHPRVYGFLHVPVQSASDSVLMDMKREYCCDDFRRVVDFLKDRVPGITIATDIICGFPGETDEDFQETMELVKKYRFPSLFINQFYPRPGTPAAK--MEQVPAQVKKQRTKELSALFH----SYNPYHHKIGERQHVLVTEES-FDAQYYVAHNKFYEQVLVPK-NPGFK------------GKMIEVDIYESGKHFMRGRPVVDAKVVT 493
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Sea Lamprey
Match: cdk5rap1 (CDK5 regulatory subunit associated protein 1 [Source:ZFIN;Acc:ZDB-GENE-070424-90])

HSP 1 Score: 443.736 bits (1140), Expect = 2.480e-151
Identity = 243/511 (47.55%), Postives = 335/511 (65.56%), Query Frame = 2 
Query:   86 FSNDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSK-----LKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSE---SNEVHGVISKEKTKLADGFSTVYK-----PKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYT-REAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMR----EKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSIATFNS 1564
            + + + VY ETYGCQMN +D+EIA +IL+  GY  T NL EA VIL +TCAIR+ AE  +W R++ +  + +   K     L+IG+LGCMAERLK  +L+   A V++V GPDAYR LP LL   ++ ++ ++VALS++ETYAD+ P+R +    PSAFVS+MRGC+NMCTYCIVPFTRG+ERSR + S+L EV  L  QG+KE+VLLGQNVNSY D S+        G      + L+ GF +V +     P     RF +LL AVS+++ E+R+RFTSPHPKDFPDEVL +IR  PNICKQ+HLPAQSGSS+VL  MGRG   R+ +L LV+ V     NV+L +  +S    +T+    L + LLK    +   C P + R    +KT+A HRL DDV   +K  R  EL AAFR+   ++N + +GS QLVL++G S+RS  E  GR + N +V+FP + LP        +   + GDYVAVKIT+++SQ+L+G  + R+++  F++
Sbjct:   42 WGHGRKVYFETYGCQMNTSDTEIAWAILQKQGYFRTDNLAEADVILAVTCAIRDKAEQTVWRRVERFRHLKRERQKRGGAALQIGILGCMAERLKRDILDRERA-VDVVAGPDAYRGLPHLLARARSHQEAIDVALSVDETYADVVPLR-ADPSAPSAFVSVMRGCNNMCTYCIVPFTRGRERSRPLASVLAEVGVLLQQGVKEVVLLGQNVNSYCDTSQPAAGTPPEGPPDGGPSALSRGFGSVCRRLTPAPDRREVRFAELLAAVSELDPEMRVRFTSPHPKDFPDEVLQVIRERPNICKQIHLPAQSGSSSVLARMGRGAQDRDPFLVLVEGVSQGPVNVSLDNQSLS--VSKTKMSW-LVVFLLK----SKHLCCPRATRACVEQKTRASHRLRDDVPPSVKQRRLSELIAAFRESASDLNAARVGSTQLVLVEGASRRSQLELSGRTDGNARVVFPDVLLPDGAGSPALASA-RRGDYVAVKITSSSSQSLRGTALHRTTLREFHA 542
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Nematostella
Match: EDO27989 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T7Q9])

HSP 1 Score: 313.153 bits (801), Expect = 3.244e-103
Identity = 156/321 (48.60%), Postives = 213/321 (66.36%), Query Frame = 2 
Query:   92 NDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSY--------RDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAY 1030
            N K ++IE+YGCQMN NDSEI  SIL   G+  T +L++A ++L+ TC+IRE AE  I  RL+ Y+ + K+++K+K+GVLGCMAERLK K LE     V+LV GPDAYRDLP LL+EI  GR  +NV LS +ETY D+ PVRL++ G+ SAFVSI RGCDNMCT+C+VPFTRG+ERSR   SIL+E+Q+++DQ  KEI LLGQNV+SY        +D  ++ E+    + +                    F  LLD  +    ++R RF++ +P+D   +V+H +    NICK +HLP QSGS+ +L+ M R +TRE Y
Sbjct:   25 NTKKLFIESYGCQMNMNDSEIVASILAQQGFNTTQHLEDADLVLVNTCSIREKAEQTIRKRLQKYNAVKKKNTKMKVGVLGCMAERLKEKFLEEE-KIVDLVVGPDAYRDLPNLLQEIDEGRDAVNVILSKDETYGDVAPVRLNNNGV-SAFVSITRGCDNMCTFCVVPFTRGRERSRDPQSILEEIQSMADQNFKEITLLGQNVDSYLWYGGGLKKDFKKATEIAQATAVD--------------------FAQLLDMAATRFPKMRFRFSTSNPQDMSLDVIHTMAKHKNICKYIHLPVQSGSNKMLKAMNRQHTREEY 323
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Nematostella
Match: EDO40392 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7F5])

HSP 1 Score: 120.168 bits (300), Expect = 7.187e-29
Identity = 124/489 (25.36%), Postives = 219/489 (44.79%), Query Frame = 2 
Query:   98 KMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPS-------AFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSY-RDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHL-----IRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKG 1525
            + +Y+ T+GC  N +DSE     L + GY  T + + A + LL +C ++  AED   + +K      K      + V GC+ +    +   +T+  +++V      + + R+++ ++   KG  V L   +  AD      +S  +P          ++I  GC N CTYC     RG   S   + I+   +   ++G+ E+ L  ++  +Y +D+       GV   E       +  V     GGR               +RI  T+P     P  + HL     I   P +   LH+P QS S+ VL +M R Y  E + ++V+ +R  +P++ + +D I GF  ETE+D Q TL L+K  ++  +F   +  R  T A  R+    ++E+K  R  E++  F+  T   ++  +G++Q VL+   S    + +VG N+A            ++  L+   E   +G  V V++T++    L G
Sbjct:   62 QTIYVRTWGCSHNNSDSEYMAGQLAAYGYKITDDEQCADLWLLNSCTVKSPAEDGFRNAIKKAKAQGKH-----LVVAGCVPQ---GQPRHDTVKGISVV----GVQQIDRVVEVVEETLKGHTVRL-FGQKKADGKKTGGASLDLPKIRKNPLVEIIAINTGCLNQCTYCKTKHARGDLGSYPPEEIVNRAKQAFNEGVVEMWLTSEDTGAYGKDI-------GVTLPELL-----WQLVKVIPEGGR---------------MRIGMTNP-----PYILEHLEEMAKILNHPRVYSFLHVPVQSASTNVLADMKREYIAEDFEHVVNYLRQRVPDLTIATDLICGFPTETEDDFQKTLDLVKKYKFPSLFINQFYPRPGTPAA-RMKRLPTEEVKR-RTREVSKLFQSYTTYDHK--VGAVQEVLVTEESHDGVH-YVGHNKA------------YDQVLVKKDEGELMGKIVKVEMTSSGKHYLMG 488
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Nematostella
Match: EDO26217 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8DV91])

HSP 1 Score: 115.931 bits (289), Expect = 1.413e-27
Identity = 85/278 (30.58%), Postives = 135/278 (48.56%), Query Frame = 2 
Query:  515 RLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDD-VSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLID 1345
            + S+   P A+     GC++  ++CI+P  RGK  SR +  +L E + L   G++E++++ Q+ ++Y          GV  K KT   +G       KT   R  ++ +A+S +   +R+ +  P+P    DE++ L+ A   I   L +P Q  S  VL+ M R    +  L  +   R I P + + S FI GF GETEED Q  L+ L   + + + C+ YS  E   A   L+D+ V D+IK  R     A  + I+    Q  +G    VLID
Sbjct:  207 KASAHNAPLAYPKFSEGCNHSGSFCIIPSMRGKLVSRPVGEVLSEAERLVKSGVRELLVISQDTSAY----------GVDVKYKTDFWNGQPV----KT---RMTEMCEALSSMGVWVRLHYVYPYPHV--DELIPLMAAG-KILPYLDIPFQHASPKVLKAMKRPAFEDKTLARIKNWREICPELTIRSTFIVGFPGETEEDFQYLLNWLTEAQLDRVGCFQYSPVEGAHA--NLLDNPVPDDIKQDRWDRFMAHQQAISTARLQLKVGKEMDVLID 462
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Nematostella
Match: EDO26538 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TBR7])

HSP 1 Score: 89.7373 bits (221), Expect = 1.569e-20
Identity = 62/212 (29.25%), Postives = 102/212 (48.11%), Query Frame = 2 
Query:   92 NDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSKLK----IGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSY 715
            N K V   T GC++NF+++       + +G+      + A + ++ TC++ E A+       K +  I K++ KL     +  +GC A+ LK + L   +  V+LV G      +   + ++   +  M    S E   AD      S      AF+ +  GCD  CTYC +P  RG  RS  ++ +LK  + +S++GIKEIVL G N+  Y
Sbjct:    3 NKKKVAFYTLGCKLNFSETSTIARNFQDEGFERVDFEEVADMYVINTCSVTENAD-------KQFKQIVKKAMKLNDKAFVAAVGCYAQ-LKPEELA-AVDGVDLVLGATEKFKITDYINDL--AKNDMGEVHSCEIEDADFYVGSYSIGDRTRAFLKVQDGCDYKCTYCTIPLARGISRSDTMEGVLKNAKEISEKGIKEIVLTGVNIGDY 203
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Nematostella
Match: EDO27803 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T891])

HSP 1 Score: 77.0258 bits (188), Expect = 3.186e-16
Identity = 63/221 (28.51%), Postives = 99/221 (44.80%), Query Frame = 2 
Query:  566 CDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSY-RDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHL-----IRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKA 1210
            C N CTYC     RG   S   + I+   +   ++G+ E+ L  ++  +Y +D+       GV   E       +  V     GGR               +RI  T+P     P  + HL     I   P +   LH+P QS S+ VL +M R Y  E + ++V+ +R  +P++ + +D I GF  ETE+D Q TL L+K  ++  +F   +  R  T A
Sbjct:    1 CLNQCTYCKTKHARGDLGSYPPEEIVNRAKQAFNEGVVEMWLTSEDTGAYGKDI-------GVTLPELL-----WQLVKVIPEGGR---------------MRIGMTNP-----PYILEHLEEMAKILNHPRVYSFLHVPVQSASTNVLADMKREYIAEDFEHVVNYLRQRVPDLTIATDLICGFPTETEDDFQKTLDLVKKYKFPSLFINQFYPRPGTPA 189
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Medaka
Match: cdk5rap1 (CDK5 regulatory subunit associated protein 1 [Source:NCBI gene;Acc:101162753])

HSP 1 Score: 582.022 bits (1499), Expect = 0.000e+0
Identity = 296/490 (60.41%), Postives = 360/490 (73.47%), Query Frame = 2 
Query:   92 NDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGI-NKR---SSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSI 1549
            N + VY ETYGCQMN ND+EIA SIL+  GY  T  L EA V+LL+TC+IRE AE  IW+RLK    + NKR    S +KIGVLGCMAERLK++LLE     V+++ GPDAYRDLPRLL   + G++  NV LSLEETYAD+ PV+L+ +G  SAFVSIMRGCDNMC+YCIVPFTRG+ERSR I SIL+EV+ LSDQG+KE+ LLGQNVNSYRD SE        S + T+L+ GF TVYKPK GG RF DLL+  S+VN  +RIRFTSPHPKDFPDEVL LI    NICKQ+HLPAQSGSS VL+ M RGYTREAYL+LV  ++ +IP V+L+SDFISGFCGETE+DHQ TLSL++ V YN  F + YSMR+KT+AFHRL DDV  E+K  R  E  + FRQ  L +N + IGS QLVL++G SKRS+ E  GR + N +V+FPK           +SE    GDYV VKI +A+SQ+L+G  +  SS+
Sbjct:   92 NSRKVYFETYGCQMNVNDTEIAWSILQKKGYERTAQLSEADVVLLVTCSIREKAEQTIWNRLKQLTAMKNKRMKTPSPMKIGVLGCMAERLKTELLEREKL-VDVLAGPDAYRDLPRLLAVAEGGQQASNVLLSLEETYADVMPVQLAPQGH-SAFVSIMRGCDNMCSYCIVPFTRGRERSRPIGSILEEVRILSDQGVKEVTLLGQNVNSYRDTSEEQ----FCSSDPTRLSRGFQTVYKPKQGGLRFSDLLERASRVNPHMRIRFTSPHPKDFPDEVLELIAERENICKQIHLPAQSGSSQVLKAMRRGYTREAYLDLVRNIKQMIPGVSLSSDFISGFCGETEDDHQQTLSLIRQVGYNVGFLFAYSMRKKTQAFHRLQDDVPAEVKRRRLEECISVFRQEALRVNAALIGSSQLVLVEGESKRSAEELCGRTDGNMRVVFPK-----EEPAGGSSEAITAGDYVRVKILSASSQSLRGRALGLSSL 570
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Medaka
Match: cdk5rap1 (CDK5 regulatory subunit associated protein 1 [Source:NCBI gene;Acc:101162753])

HSP 1 Score: 582.022 bits (1499), Expect = 0.000e+0
Identity = 296/490 (60.41%), Postives = 360/490 (73.47%), Query Frame = 2 
Query:   92 NDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGI-NKR---SSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSI 1549
            N + VY ETYGCQMN ND+EIA SIL+  GY  T  L EA V+LL+TC+IRE AE  IW+RLK    + NKR    S +KIGVLGCMAERLK++LLE     V+++ GPDAYRDLPRLL   + G++  NV LSLEETYAD+ PV+L+ +G  SAFVSIMRGCDNMC+YCIVPFTRG+ERSR I SIL+EV+ LSDQG+KE+ LLGQNVNSYRD SE        S + T+L+ GF TVYKPK GG RF DLL+  S+VN  +RIRFTSPHPKDFPDEVL LI    NICKQ+HLPAQSGSS VL+ M RGYTREAYL+LV  ++ +IP V+L+SDFISGFCGETE+DHQ TLSL++ V YN  F + YSMR+KT+AFHRL DDV  E+K  R  E  + FRQ  L +N + IGS QLVL++G SKRS+ E  GR + N +V+FPK           +SE    GDYV VKI +A+SQ+L+G  +  SS+
Sbjct:   92 NSRKVYFETYGCQMNVNDTEIAWSILQKKGYERTAQLSEADVVLLVTCSIREKAEQTIWNRLKQLTAMKNKRMKTPSPMKIGVLGCMAERLKTELLEREKL-VDVLAGPDAYRDLPRLLAVAEGGQQASNVLLSLEETYADVMPVQLAPQGH-SAFVSIMRGCDNMCSYCIVPFTRGRERSRPIGSILEEVRILSDQGVKEVTLLGQNVNSYRDTSEEQ----FCSSDPTRLSRGFQTVYKPKQGGLRFSDLLERASRVNPHMRIRFTSPHPKDFPDEVLELIAERENICKQIHLPAQSGSSQVLKAMRRGYTREAYLDLVRNIKQMIPGVSLSSDFISGFCGETEDDHQQTLSLIRQVGYNVGFLFAYSMRKKTQAFHRLQDDVPAEVKRRRLEECISVFRQEALRVNAALIGSSQLVLVEGESKRSAEELCGRTDGNMRVVFPK-----EEPAGGSSEAITAGDYVRVKILSASSQSLRGRALGLSSL 570
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Medaka
Match: cdk5rap1 (CDK5 regulatory subunit associated protein 1 [Source:NCBI gene;Acc:101162753])

HSP 1 Score: 524.628 bits (1350), Expect = 0.000e+0
Identity = 276/483 (57.14%), Postives = 333/483 (68.94%), Query Frame = 2 
Query:   92 NDKMVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGI-NKR---SSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGD 1528
            N + VY ETYGCQMN ND+EIA SIL+  GY  T  L EA V+LL+TC+IRE AE  IW+RLK    + NKR    S +KIGVLGCMAERLK++LLE     V+++ GPDAYRDLPRLL   + G++  NV LSLEETYAD+ PV+L+ +G  SAFVSIMRGCDNMC+YCIVPFTRG+ERSR I SIL+EV+ LSDQG+KE+ LLGQNVNSYRD SE        S + T+L+ GF TVYKPK GG RF DLL+  S+VN  +RIRFTSPHPKDFPDEVL LI    NICKQ+HLPAQSGSS                      +  +P V+L+SDFISGFCGETE+DHQ TLSL++ V YN  F + YSMR+KT+AFHRL DDV  E+K  R  E  + FRQ  L +N + IGS QLVL++G SKRS+ E  GR + N +V+FPK           +SE    GDYV VK+     +TL  D
Sbjct:   92 NSRKVYFETYGCQMNVNDTEIAWSILQKKGYERTAQLSEADVVLLVTCSIREKAEQTIWNRLKQLTAMKNKRMKTPSPMKIGVLGCMAERLKTELLEREKL-VDVLAGPDAYRDLPRLLAVAEGGQQASNVLLSLEETYADVMPVQLAPQGH-SAFVSIMRGCDNMCSYCIVPFTRGRERSRPIGSILEEVRILSDQGVKEVTLLGQNVNSYRDTSEEQ----FCSSDPTRLSRGFQTVYKPKQGGLRFSDLLERASRVNPHMRIRFTSPHPKDFPDEVLELIAERENICKQIHLPAQSGSS----------------------QRFLPCVSLSSDFISGFCGETEDDHQQTLSLIRQVGYNVGFLFAYSMRKKTQAFHRLQDDVPAEVKRRRLEECISVFRQEALRVNAALIGSSQLVLVEGESKRSAEELCGRTDGNMRVVFPK-----EEPAGGSSEAITAGDYVRVKV--GGDRTLAAD 539
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Medaka
Match: cdkal1 (CDK5 regulatory subunit associated protein 1 like 1 [Source:NCBI gene;Acc:101158380])

HSP 1 Score: 129.798 bits (325), Expect = 8.304e-32
Identity = 128/501 (25.55%), Postives = 217/501 (43.31%), Query Frame = 2 
Query:  104 VYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVAL--SLEETYADITPVRLSSKGIPS----AFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSY-RDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHL-----IRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSIATFNSEI 1570
            ++++T+GC  N +D E     L + GY  T +  EA + LL +C ++  AED   + +K     +K     K+ V GC+ +         +++ + +       + + R+++ +    KG +V L    +E    +   RL    I        +SI  GC N CTYC     RG   S  I+ ++K V+    +G+ EI L  ++  +Y RD+    ++  ++ +   ++ DG                           +R+  T+P     P  + HL     I   P +   LH+P QS S +VL +M R Y  + +  +VD ++  +P V + +D I GF GETEED Q TL L+K  R+  +F   +  R  T A    ++ V  ++K  R  EL+  F   +       +G +Q VL+   S   +  +V  N+   +V+ PK  + F             G  + V+I  A    +KG P+  S    FN  I
Sbjct:   61 IWMKTWGCSHNNSDGEYMAGQLAASGYKMTDDPAEADLWLLNSCTVKNPAEDHFRNSIKKAQEQDK-----KVVVAGCVPQAQPRMDYLKSLSIIGV-------QQIDRVVEVVDEAVKGHSVRLLGQKKEGGRRLGGARLDLPKIRKNPLIEIISINTGCLNACTYCKTKHARGDLASYPIEELVKRVRQSFQEGVCEIWLTSEDTGAYGRDIG--TDLPTLLWRLVEQIPDGAM-------------------------LRLGMTNP-----PYILEHLGEMAKILNHPRVYAFLHVPVQSASDSVLMDMKREYCIDDFKRVVDFLKERVPGVTIATDIICGFPGETEEDFQETLELVKLYRFPSLFINQFYPRPGTPAAK--MEQVPAQVKKQRTRELSQLFH--SYNPYDHKVGEMQHVLVTEES-FDAQYYVAHNKFYEQVLVPK-KVEFK------------GKMIEVEIYEAGKHFMKGRPVEDSK--PFNPSI 497
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Medaka
Match: cdkal1 (CDK5 regulatory subunit associated protein 1 like 1 [Source:NCBI gene;Acc:101158380])

HSP 1 Score: 129.798 bits (325), Expect = 8.304e-32
Identity = 128/501 (25.55%), Postives = 217/501 (43.31%), Query Frame = 2 
Query:  104 VYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVAL--SLEETYADITPVRLSSKGIPS----AFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSY-RDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHL-----IRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSIATFNSEI 1570
            ++++T+GC  N +D E     L + GY  T +  EA + LL +C ++  AED   + +K     +K     K+ V GC+ +         +++ + +       + + R+++ +    KG +V L    +E    +   RL    I        +SI  GC N CTYC     RG   S  I+ ++K V+    +G+ EI L  ++  +Y RD+    ++  ++ +   ++ DG                           +R+  T+P     P  + HL     I   P +   LH+P QS S +VL +M R Y  + +  +VD ++  +P V + +D I GF GETEED Q TL L+K  R+  +F   +  R  T A    ++ V  ++K  R  EL+  F   +       +G +Q VL+   S   +  +V  N+   +V+ PK  + F             G  + V+I  A    +KG P+  S    FN  I
Sbjct:   61 IWMKTWGCSHNNSDGEYMAGQLAASGYKMTDDPAEADLWLLNSCTVKNPAEDHFRNSIKKAQEQDK-----KVVVAGCVPQAQPRMDYLKSLSIIGV-------QQIDRVVEVVDEAVKGHSVRLLGQKKEGGRRLGGARLDLPKIRKNPLIEIISINTGCLNACTYCKTKHARGDLASYPIEELVKRVRQSFQEGVCEIWLTSEDTGAYGRDIG--TDLPTLLWRLVEQIPDGAM-------------------------LRLGMTNP-----PYILEHLGEMAKILNHPRVYAFLHVPVQSASDSVLMDMKREYCIDDFKRVVDFLKERVPGVTIATDIICGFPGETEEDFQETLELVKLYRFPSLFINQFYPRPGTPAAK--MEQVPAQVKKQRTRELSQLFH--SYNPYDHKVGEMQHVLVTEES-FDAQYYVAHNKFYEQVLVPK-KVEFK------------GKMIEVEIYEAGKHFMKGRPVEDSK--PFNPSI 497
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Planmine SMEST
Match: SMESG000038959.1 (SMESG000038959.1)

HSP 1 Score: 1051.2 bits (2717), Expect = 0.000e+0
Identity = 507/509 (99.61%), Postives = 507/509 (99.61%), Query Frame = 2 
Query:  101 MVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPICRSSIATFNSEIINSGNNTISQNKIPHQCYS 1627
            MVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPK GGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKI AATSQTLKGDPICRSSIATFNSEIINSGNNTISQNKIPHQCYS
Sbjct:    1 MVYIETYGCQMNFNDSEIAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGVISKEKTKLADGFSTVYKPKIGGRRFVDLLDAVSQVNKEIRIRFTSPHPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKIIAATSQTLKGDPICRSSIATFNSEIINSGNNTISQNKIPHQCYS 509
[Close]
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Planmine SMEST
Match: SMESG000011997.1 (SMESG000011997.1)

HSP 1 Score: 89.3521 bits (220), Expect = 1.562e-18
Identity = 119/521 (22.84%), Postives = 213/521 (40.88%), Query Frame = 2 
Query:  104 VYIETYGCQMNFNDSEIAMSILKSDGYT------------------HTTNLKE----------ASVILLMTCAIREGAEDKIWDRLKYYHGINKRSSKLKIGVLGCMAE-RLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKAGRKGMNVAL--SLEETYADITPVRLSSKGI-PSAFVSIM---RGCDNMCTYCIVPFTRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSY-RDMSESNEVHGVISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQ---VNKEIRIRFTSP-HPKDFPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKVRVII--PNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREKTKA--FHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGTSKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITAATSQTLKGDPI 1534
            +YI+T+GC  N +DSE    IL   G+                   H   + E          A + +L +C ++  +ED   + +      NK     K+ V GC+ + + K+  L+     V+++      + + R+++ ++   KG +V       E    +   +L+   I  + F+ I+    GC N CTYC     RG   S  I  I+  V     +   EI L  +++ +Y RD+                              G    +LL+A+ +       +R+  T+P +  +  + + H+++  P +   LH+P QSGS  VL  M R YTRE + N+VD +   I    V + +D I GF  ET+ED + T SL+   R++ +F   +  R  T A    R+   +  + ++ R  EL  ++   T       +G    +LI   S      +VG  +A  +V+  ++                +G    V++T  +  ++K + I
Sbjct:   53 IYIQTWGCSHNSSDSEYMAGILSKYGFKVSLNGSCSLKGDGDEIVLHNKKVIEKSSDEDNKLIADLWILNSCTVKNPSEDHFRNMINEARKYNK-----KVIVAGCVPQGQPKAPYLKG----VSVI----GVQQIDRVVEVVEETLKGNSVQFYSKKREGGKMLGGAKLNLPKIRKNPFIEILPINTGCLNACTYCKTKHARGHLGSYPISEIVDRVDQAFSENCVEIWLTSEDLGAYGRDI------------------------------GTNLPELLEAIVEHIPSGCMLRLGMTNPPYILEHLERIAHILQH-PRVYSFLHVPVQSGSDNVLGEMKREYTREDFCNVVDTLYQTIGRTKVNIATDIICGFPTETDEDFEETYSLVDKYRFSVLFNNQFYPRPGTPAARMERVASSLEVKKRTKRIHELFQSYEPYT-----DRVGEEMNILITEEST-DGKYFVGHTKAYEQVLVARVH-------------QLMGKIARVRVTETSKHSMKAEII 510
The following BLAST results are available for this feature:
BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CDK5RAP10.000e+057.81CDK5 regulatory subunit associated protein 1 [Sour... [more]
CDK5RAP10.000e+057.81CDK5 regulatory subunit associated protein 1 [Sour... [more]
CDK5RAP10.000e+056.21CDK5 regulatory subunit associated protein 1 [Sour... [more]
CDK5RAP18.880e-7358.33CDK5 regulatory subunit associated protein 1 [Sour... [more]
CDK5RAP11.698e-5549.24CDK5 regulatory subunit associated protein 1 [Sour... [more]
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BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
F25B5.55.154e-17751.29CDK5RAP1-like protein [Source:UniProtKB/Swiss-Pro... [more]
Y92H12BL.16.572e-1923.88Threonylcarbamoyladenosine tRNA methylthiotransfer... [more]
Y92H12BL.16.572e-1923.88Threonylcarbamoyladenosine tRNA methylthiotransfer... [more]
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BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CG63450.000e+053.83gene:FBgn0037816 transcript:FBtr0082258[more]
CG65504.331e-2624.23gene:FBgn0034214 transcript:FBtr0086961[more]
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BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
cdk5rap10.000e+059.18CDK5 regulatory subunit associated protein 1 [Sour... [more]
cdk5rap10.000e+059.18CDK5 regulatory subunit associated protein 1 [Sour... [more]
cdk5rap10.000e+059.30CDK5 regulatory subunit associated protein 1 [Sour... [more]
cdkal12.998e-1628.57CDK5 regulatory subunit associated protein 1 like ... [more]
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BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cdk5rap10.000e+058.14CDK5 regulatory subunit associated protein 1 [Sour... [more]
cdk5rap13.640e-17057.92CDK5 regulatory subunit associated protein 1 [Sour... [more]
cwf19l19.439e-2924.71CWF19 like cell cycle control factor 1 [Source:Xen... [more]
cwf19l11.065e-2824.71CWF19 like cell cycle control factor 1 [Source:Xen... [more]
cwf19l11.438e-2824.71CWF19 like cell cycle control factor 1 [Source:Xen... [more]
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BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Cdk5rap10.000e+057.78CDK5 regulatory subunit associated protein 1 [Sour... [more]
Cdk5rap10.000e+057.78CDK5 regulatory subunit associated protein 1 [Sour... [more]
Cdkal12.613e-2824.47CDK5 regulatory subunit associated protein 1-like ... [more]
Cdkal13.501e-2824.47CDK5 regulatory subunit associated protein 1-like ... [more]
Cdk5rap14.441e-2351.72CDK5 regulatory subunit associated protein 1 [Sour... [more]
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BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q8BTW8|CK5P1_MOUSE0.000e+057.78Mitochondrial tRNA methylthiotransferase CDK5RAP1 ... [more]
sp|Q9JLH6|CK5P1_RAT0.000e+057.37Mitochondrial tRNA methylthiotransferase CDK5RAP1 ... [more]
sp|Q96SZ6|CK5P1_HUMAN0.000e+056.21Mitochondrial tRNA methylthiotransferase CDK5RAP1 ... [more]
sp|Q9VGZ1|CK5P1_DROME0.000e+053.83CDK5RAP1-like protein OS=Drosophila melanogaster O... [more]
sp|Q2R1U4|CK5P1_ORYSJ7.925e-17952.10CDK5RAP1-like protein OS=Oryza sativa subsp. japon... [more]
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BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3S1BF200.000e+059.43Uncharacterized protein OS=Elysia chlorotica OX=18... [more]
A0A1S3JD810.000e+059.07CDK5 regulatory subunit-associated protein 1 OS=Li... [more]
A0A210QJX10.000e+059.64CDK5 regulatory subunit-associated protein 1 OS=Mi... [more]
R7TUC40.000e+059.24Uncharacterized protein OS=Capitella teleta OX=283... [more]
A0A2C9JX730.000e+059.07Uncharacterized protein OS=Biomphalaria glabrata O... [more]
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BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
CDK5RAP10.000e+058.57CDK5 regulatory subunit associated protein 1 [Sour... [more]
CDK5RAP10.000e+057.52CDK5 regulatory subunit associated protein 1 [Sour... [more]
cdkal11.588e-2223.67CDK5 regulatory subunit associated protein 1 like ... [more]
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BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
cdk5rap12.480e-15147.55CDK5 regulatory subunit associated protein 1 [Sour... [more]
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BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO279893.244e-10348.60Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO403927.187e-2925.36Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO262171.413e-2730.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8... [more]
EDO265381.569e-2029.25Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO278033.186e-1628.51Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cdk5rap10.000e+060.41CDK5 regulatory subunit associated protein 1 [Sour... [more]
cdk5rap10.000e+060.41CDK5 regulatory subunit associated protein 1 [Sour... [more]
cdk5rap10.000e+057.14CDK5 regulatory subunit associated protein 1 [Sour... [more]
cdkal18.304e-3225.55CDK5 regulatory subunit associated protein 1 like ... [more]
cdkal18.304e-3225.55CDK5 regulatory subunit associated protein 1 like ... [more]
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BLAST of tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000038959.10.000e+099.61SMESG000038959.1[more]
SMESG000011997.11.562e-1822.84SMESG000011997.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015224 ID=SMED30015224|Name=tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase|organism=Schmidtea mediterranea sexual|type=transcript|length=1645bp
AATGAATTATATTTGTATGAAAAATTTTTTTAATTATAATAAAAAATATT
TACATTATTCCAACATCCAAAGACTCTGTTCAAATTTTTCTAATGATAAA
ATGGTTTATATCGAAACATATGGATGCCAAATGAATTTTAATGATAGTGA
AATAGCCATGTCGATATTAAAATCTGACGGTTATACACATACCACTAATT
TAAAAGAAGCTAGTGTTATTCTATTAATGACTTGCGCGATAAGAGAAGGA
GCCGAAGACAAAATTTGGGATAGATTAAAATATTATCATGGAATTAATAA
AAGATCATCTAAACTCAAAATTGGTGTACTCGGTTGCATGGCAGAAAGAT
TAAAATCAAAATTACTTGAAAACACAATAGCGCCTGTGAATTTAGTCTGT
GGTCCGGATGCATATCGAGATTTACCACGTTTATTAAAGGAAATTAAAGC
TGGAAGGAAAGGAATGAATGTAGCATTGTCATTAGAAGAAACATATGCTG
ATATTACTCCGGTTAGATTGAGCAGTAAGGGAATTCCATCAGCTTTCGTT
TCAATAATGCGTGGCTGTGATAATATGTGTACGTATTGTATTGTTCCTTT
CACAAGAGGAAAAGAACGTAGCCGTCGTATTGATTCGATTTTAAAAGAAG
TTCAAACACTTTCAGATCAAGGAATTAAAGAAATTGTTTTATTGGGGCAA
AATGTAAATAGTTATCGAGATATGAGTGAATCGAATGAAGTTCATGGTGT
AATTTCCAAAGAAAAAACTAAATTGGCAGATGGATTTTCAACTGTATATA
AACCCAAAACCGGCGGTAGAAGATTTGTTGATTTGCTAGACGCAGTTAGT
CAAGTAAATAAAGAAATTCGAATTCGGTTTACTTCTCCACATCCTAAAGA
TTTTCCTGACGAGGTGCTTCACCTAATAAGAGCTACTCCTAATATCTGTA
AACAACTTCATCTACCTGCTCAAAGTGGAAGCTCTACTGTCCTTCAGAAC
ATGGGACGTGGCTATACAAGGGAAGCCTATTTAAATCTTGTTGATAAAGT
TCGAGTTATAATTCCCAATGTAGCGCTGACCAGTGATTTTATTAGTGGAT
TTTGCGGTGAAACAGAAGAGGATCATCAACTAACATTGTCATTATTGAAA
TATGTCAGATATAATTTCATTTTTTGCTATCCCTACAGTATGAGAGAGAA
AACAAAAGCATTTCATCGATTGATCGATGATGTCAGTGATGAAATAAAGT
CTTGCAGACATTTAGAATTGGCAGCAGCATTCCGTCAAATCACCTTGGAA
ATTAACCAATCACATATCGGCTCAATTCAACTGGTGTTGATCGATGGGAC
AAGTAAAAGATCATCAAATGAATGGGTAGGGAGAAATGAGGCCAACACCA
AAGTTATTTTTCCAAAATTATCTTTGCCATTCAATAATGATCTTATAACA
AATAGTGAAATTCCTCAGATCGGAGATTACGTAGCGGTCAAAATCACTGC
TGCTACGTCACAAACTCTGAAAGGAGATCCTATTTGTCGTTCATCTATTG
CGACTTTTAATTCAGAAATCATAAATTCTGGCAATAACACAATCTCACAA
AACAAAATTCCTCATCAATGTTATAGTTAAATATAGGTTTCTGAC
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protein sequence of SMED30015224-orf-1

>SMED30015224-orf-1 ID=SMED30015224-orf-1|Name=SMED30015224-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=543bp
MNYICMKNFFNYNKKYLHYSNIQRLCSNFSNDKMVYIETYGCQMNFNDSE
IAMSILKSDGYTHTTNLKEASVILLMTCAIREGAEDKIWDRLKYYHGINK
RSSKLKIGVLGCMAERLKSKLLENTIAPVNLVCGPDAYRDLPRLLKEIKA
GRKGMNVALSLEETYADITPVRLSSKGIPSAFVSIMRGCDNMCTYCIVPF
TRGKERSRRIDSILKEVQTLSDQGIKEIVLLGQNVNSYRDMSESNEVHGV
ISKEKTKLADGFSTVYKPKTGGRRFVDLLDAVSQVNKEIRIRFTSPHPKD
FPDEVLHLIRATPNICKQLHLPAQSGSSTVLQNMGRGYTREAYLNLVDKV
RVIIPNVALTSDFISGFCGETEEDHQLTLSLLKYVRYNFIFCYPYSMREK
TKAFHRLIDDVSDEIKSCRHLELAAAFRQITLEINQSHIGSIQLVLIDGT
SKRSSNEWVGRNEANTKVIFPKLSLPFNNDLITNSEIPQIGDYVAVKITA
ATSQTLKGDPICRSSIATFNSEIINSGNNTISQNKIPHQCYS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0035596methylthiotransferase activity
GO:0003824catalytic activity
GO:0051536iron-sulfur cluster binding
GO:00515394 iron, 4 sulfur cluster binding
GO:0016740transferase activity
GO:0046872metal ion binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR038135Methylthiotransferase_N_sf
IPR005839Methylthiotransferase
IPR006463MiaB_methiolase
IPR006638Elp3/MiaB/NifB
IPR007197rSAM
IPR013848Methylthiotransferase_N
IPR002792TRAM_dom
IPR023404rSAM_horseshoe
IPR020612Methylthiotransferase_CS
Vocabulary: biological process
TermDefinition
GO:0009451RNA modification
GO:0006400tRNA modification
GO:0008033tRNA processing
GO:0043412macromolecule modification
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006638Elp3/MiaB/NifBSMARTSM00729MiaBcoord: 179..426
e-value: 4.4E-50
score: 182.4
IPR038135Methylthiotransferase, N-terminal domain superfamilyGENE3DG3DSA:3.40.50.12160coord: 34..165
e-value: 3.6E-29
score: 103.2
IPR005839MethylthiotransferaseTIGRFAMTIGR00089TIGR00089coord: 35..507
e-value: 7.7E-117
score: 388.6
IPR013848Methylthiotransferase, N-terminalPFAMPF00919UPF0004coord: 35..133
e-value: 1.8E-26
score: 92.1
IPR013848Methylthiotransferase, N-terminalPROSITEPS51449MTTASE_Ncoord: 33..150
score: 32.036
IPR007197Radical SAMPFAMPF04055Radical_SAMcoord: 183..382
e-value: 5.4E-30
score: 105.0
IPR007197Radical SAMSFLDSFLDS00029Radical_SAMcoord: 30..515
e-value: 0.0
score: 676.3
IPR023404Radical SAM, alpha/beta horseshoeGENE3DG3DSA:3.80.30.20coord: 178..439
e-value: 3.1E-81
score: 274.3
IPR002792TRAM domainPFAMPF01938TRAMcoord: 437..510
e-value: 5.8E-12
score: 45.4
IPR002792TRAM domainPROSITEPS50926TRAMcoord: 436..512
score: 12.722
NoneNo IPR availableSFLDSFLDG01082B12-binding_domain_containingcoord: 128..397
e-value: 5.7E-24
score: 79.2
NoneNo IPR availableSFLDSFLDF00413CDK5RAP1coord: 30..515
e-value: 0.0
score: 676.3
NoneNo IPR availablePANTHERPTHR43020FAMILY NOT NAMEDcoord: 28..521
NoneNo IPR availablePANTHERPTHR43020:SF2CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 1coord: 28..521
NoneNo IPR availableSUPERFAMILYSSF102114Radical SAM enzymescoord: 180..430
IPR006463tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase MiaBSFLDSFLDF00273(dimethylallyl)adenosine_tRNAcoord: 31..513
e-value: 0.0
score: 529.6
IPR020612Methylthiotransferase, conserved sitePROSITEPS01278MTTASE_RADICALcoord: 183..203