SMED30015220

Overview
NameSMED30015220
Smed IDSMED30015220
Length (bp)1926
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30015220 (SMED30015220) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30015220 (SMED30015220) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30015220 (SMED30015220) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30015220 (SMED30015220) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30015220

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30015220SMESG000027150.1 SmedASXL_014502SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30015220SMESG000027150.1 SmedASXL_014502SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30015220SMESG000027150.1 dd_Smed_v4_4740_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30015220 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000757:94657..103681 +9340
Homology
[Close]
BLAST of SMED30015220 vs. Ensembl Sea Lamprey
Match: lrpprc (leucine-rich pentatricopeptide repeat containing [Source:ZFIN;Acc:ZDB-GENE-030131-7887])

HSP 1 Score: 52.7582 bits (125), Expect = 2.236e-7
Identity = 44/168 (26.19%), Postives = 79/168 (47.02%), Query Frame = 1 
Query:  331 IKEQDLANSVSVYNALINSQVXXXXXXXXXTILSEISKKKITFNQDTYKILIKYYAKRGNLDAIELIVSKMKSMNLTVSNKQLMIILSAYYVNGLQEKAQMFIKENNMDENGIYVT----LSKAKSYATLGDVNNATKHLFEIYKNDLSFSSKYCFTIYSLLVAAGTP 822
            +KE      VS YNALI   +  +  F     L ++ K+ +T N+ TY+ LI  Y   G++D    I+  MK+ ++ ++ +    +++ +   G  + +   +  + M + GI  T    L+   +YA  GD+ + T+H   + KN+ S   +    I   L  AG P
Sbjct:   72 LKELGTVFDVSHYNALIKVYLQNEHKFSPTDFLEKMEKEGVTPNRVTYQRLIAAYCNEGDIDGASKILGFMKNKDIPITEQVFNSLVTGHAKAGDMDNSMKIL--SVMRDAGIEPTSETYLALLIAYAERGDIKSITEHFITMEKNNKSLVDRDIMQIIFSLAKAGFP 237
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BLAST of SMED30015220 vs. Planmine SMEST
Match: SMESG000027150.1 (SMESG000027150.1)

HSP 1 Score: 1035.02 bits (2675), Expect = 0.000e+0
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 1 
Query:   82 MAFFATIRLMIVSKSLTEPIIRNLLKFTPNVSSLKDEYVLYLKNLNIPLNKHNAFELLEFPYKSFHMFSDIDMKKIIDESFKIIKEQDLANSVSVYNALINSQVXXXXXXXXXTILSEISKKKITFNQDTYKILIKYYAKRGNLDAIELIVSKMKSMNLTVSNKQLMIILSAYYVNGLQEKAQMFIKENNMDENGIYVTLSKAKSYATLGDVNNATKHLFEIYKNDLSFSSKYCFTIYSLLVAAGTPKPQLEXXXXXXXXXXXXXPIYIHFAIQKLNKAKCFDQSLIFLKDFLNFVDETTAFYLIELFTIPACPXXXXXXXXXXXXCRIGTTNNVFNAFSNXXXXXXXXXXXXXSNERNIECICKSLINKALREKSLENIEASINFTMHNMFWTRVNLILQHMVLPFAVELKIVSNHEEILSLFPQFNNIFLSVILMNYLTYGKFKEYDTLRKKYLHLTNVPFMAELGYLCKTISDNLSPKEFEQFESEGKKYFTEKCIRNFVHKASFYRIVNLLKTNQDAKEMFSLLERLNYQFQEDDFNFLKIKCPDKIEVFKPFFINTIQVTNS 1782
            MAFFATIRLMIVSKSLTEPIIRNLLKFTPNVSSLKDEYVLYLKNLNIPLNKHNAFELLEFPYKSFHMFSDIDMKKIIDESFKIIKEQDLANSVSVYNALINSQVDFDIDFDIDTILSEISKKKITFNQDTYKILIKYYAKRGNLDAIELIVSKMKSMNLTVSNKQLMIILSAYYVNGLQEKAQMFIKENNMDENGIYVTLSKAKSYATLGDVNNATKHLFEIYKNDLSFSSKYCFTIYSLLVAAGTPKPQLEKIIKVMKKMIVKKPIYIHFAIQKLNKAKCFDQSLIFLKDFLNFVDETTAFYLIELFTIPACPKKVYKNVLKLLLCRIGTTNNVFNAFSNMEFEEAMEFAVAESNERNIECICKSLINKALREKSLENIEASINFTMHNMFWTRVNLILQHMVLPFAVELKIVSNHEEILSLFPQFNNIFLSVILMNYLTYGKFKEYDTLRKKYLHLTNVPFMAELGYLCKTISDNLSPKEFEQFESEGKKYFTEKCIRNFVHKASFYRIVNLLKTNQDAKEMFSLLERLNYQFQEDDFNFLKIKCPDKIEVFKPFFINTIQVTNS
Sbjct:    1 MAFFATIRLMIVSKSLTEPIIRNLLKFTPNVSSLKDEYVLYLKNLNIPLNKHNAFELLEFPYKSFHMFSDIDMKKIIDESFKIIKEQDLANSVSVYNALINSQVDFDIDFDIDTILSEISKKKITFNQDTYKILIKYYAKRGNLDAIELIVSKMKSMNLTVSNKQLMIILSAYYVNGLQEKAQMFIKENNMDENGIYVTLSKAKSYATLGDVNNATKHLFEIYKNDLSFSSKYCFTIYSLLVAAGTPKPQLEKIIKVMKKMIVKKPIYIHFAIQKLNKAKCFDQSLIFLKDFLNFVDETTAFYLIELFTIPACPKKVYKNVLKLLLCRIGTTNNVFNAFSNMEFEEAMEFAVAESNERNIECICKSLINKALREKSLENIEASINFTMHNMFWTRVNLILQHMVLPFAVELKIVSNHEEILSLFPQFNNIFLSVILMNYLTYGKFKEYDTLRKKYLHLTNVPFMAELGYLCKTISDNLSPKEFEQFESEGKKYFTEKCIRNFVHKASFYRIVNLLKTNQDAKEMFSLLERLNYQFQEDDFNFLKIKCPDKIEVFKPFFINTIQVTNS 567
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BLAST of SMED30015220 vs. Planmine SMEST
Match: SMESG000027150.1 (SMESG000027150.1)

HSP 1 Score: 1015.76 bits (2625), Expect = 0.000e+0
Identity = 558/558 (100.00%), Postives = 558/558 (100.00%), Query Frame = 1 
Query:  109 MIVSKSLTEPIIRNLLKFTPNVSSLKDEYVLYLKNLNIPLNKHNAFELLEFPYKSFHMFSDIDMKKIIDESFKIIKEQDLANSVSVYNALINSQVXXXXXXXXXTILSEISKKKITFNQDTYKILIKYYAKRGNLDAIELIVSKMKSMNLTVSNKQLMIILSAYYVNGLQEKAQMFIKENNMDENGIYVTLSKAKSYATLGDVNNATKHLFEIYKNDLSFSSKYCFTIYSLLVAAGTPKPQLEXXXXXXXXXXXXXPIYIHFAIQKLNKAKCFDQSLIFLKDFLNFVDETTAFYLIELFTIPACPXXXXXXXXXXXXCRIGTTNNVFNAFSNXXXXXXXXXXXXXSNERNIECICKSLINKALREKSLENIEASINFTMHNMFWTRVNLILQHMVLPFAVELKIVSNHEEILSLFPQFNNIFLSVILMNYLTYGKFKEYDTLRKKYLHLTNVPFMAELGYLCKTISDNLSPKEFEQFESEGKKYFTEKCIRNFVHKASFYRIVNLLKTNQDAKEMFSLLERLNYQFQEDDFNFLKIKCPDKIEVFKPFFINTIQVTNS 1782
            MIVSKSLTEPIIRNLLKFTPNVSSLKDEYVLYLKNLNIPLNKHNAFELLEFPYKSFHMFSDIDMKKIIDESFKIIKEQDLANSVSVYNALINSQVDFDIDFDIDTILSEISKKKITFNQDTYKILIKYYAKRGNLDAIELIVSKMKSMNLTVSNKQLMIILSAYYVNGLQEKAQMFIKENNMDENGIYVTLSKAKSYATLGDVNNATKHLFEIYKNDLSFSSKYCFTIYSLLVAAGTPKPQLEKIIKVMKKMIVKKPIYIHFAIQKLNKAKCFDQSLIFLKDFLNFVDETTAFYLIELFTIPACPKKVYKNVLKLLLCRIGTTNNVFNAFSNMEFEEAMEFAVAESNERNIECICKSLINKALREKSLENIEASINFTMHNMFWTRVNLILQHMVLPFAVELKIVSNHEEILSLFPQFNNIFLSVILMNYLTYGKFKEYDTLRKKYLHLTNVPFMAELGYLCKTISDNLSPKEFEQFESEGKKYFTEKCIRNFVHKASFYRIVNLLKTNQDAKEMFSLLERLNYQFQEDDFNFLKIKCPDKIEVFKPFFINTIQVTNS
Sbjct:    1 MIVSKSLTEPIIRNLLKFTPNVSSLKDEYVLYLKNLNIPLNKHNAFELLEFPYKSFHMFSDIDMKKIIDESFKIIKEQDLANSVSVYNALINSQVDFDIDFDIDTILSEISKKKITFNQDTYKILIKYYAKRGNLDAIELIVSKMKSMNLTVSNKQLMIILSAYYVNGLQEKAQMFIKENNMDENGIYVTLSKAKSYATLGDVNNATKHLFEIYKNDLSFSSKYCFTIYSLLVAAGTPKPQLEKIIKVMKKMIVKKPIYIHFAIQKLNKAKCFDQSLIFLKDFLNFVDETTAFYLIELFTIPACPKKVYKNVLKLLLCRIGTTNNVFNAFSNMEFEEAMEFAVAESNERNIECICKSLINKALREKSLENIEASINFTMHNMFWTRVNLILQHMVLPFAVELKIVSNHEEILSLFPQFNNIFLSVILMNYLTYGKFKEYDTLRKKYLHLTNVPFMAELGYLCKTISDNLSPKEFEQFESEGKKYFTEKCIRNFVHKASFYRIVNLLKTNQDAKEMFSLLERLNYQFQEDDFNFLKIKCPDKIEVFKPFFINTIQVTNS 558
[Close]
BLAST of SMED30015220 vs. Planmine SMEST
Match: SMESG000026537.1 (SMESG000026537.1)

HSP 1 Score: 152.525 bits (384), Expect = 3.670e-39
Identity = 155/566 (27.39%), Postives = 281/566 (49.65%), Query Frame = 1 
Query:   82 MAFFATIRLMIVSKSL-TEPIIRNLLKFTPNVSSLKDEYVLYLKNLNIPLNKHNAFELLEFPYKSFHMFSDIDMKKIIDESFKIIKEQDLANSVSVYNALINSQVXXXXXXXXXTILSEISKKKITFNQDTYKILIKYYAKRGNLDAIELIVSKMKSMNLTVSNKQLMIILSAYYVNGLQEKAQMFIKENNMDENGIYVTLSKAKSYATLGDVNNATKHLFEIYKNDLSFSSKYCFT-IYSLLVAAGTPKPQLEXXXXXXXXXXXXXPIYIHFAIQKLNKAKCFDQSLIFLKDFLNFVDETTAFYLIELFTIPACPXXXXXXXXXXXXC---RIGTTNNVFNAFSNXXXXXXXXXXXXXSNERNIECICKSLINKALREKSLENIEASINFTMHNMFWTRVNLILQHMVLPFAVELKIVSNHEEILSLFPQFNNIFLSVILMNYLTYGKFKEYDTLRKKYLHLTNVPFMAELGYLCKTISDNLSPKEFEQFESEGKKYFTEKCIRNFVHKASFYRIVNLLKTNQDAKEMFS----LLERLNYQFQEDDFNFLKIKCPDKIEVFKPF 1752
            MA   T RL I ++ L TE I+++L K   NV     +YV  +K +N+      + EL++FP+KSF+ F    +K +I+ES  I+KE+  A ++ VYN ++ S     I+FD+D  L +I + K+  N +T+ ++IK Y++   +D IE ++ KM+  N+ +  K L  ++ AY V G +EK + F+K N +DE+ + + ++K ++YA +GD    T  LF++  +    +  +  + +Y ++         L+ +I +MK+ ++ + I +      +N  K +++ L  L+  +  + +   +  +  F  P CP  +YKNVL L L    R+ T +NVF   S  +       A + +  R    +  SL+NKA+  KS +NI  ++ F    +     + ++Q +++   +  K+V +  E++S+F       L  +++ ++  G F E   L   +       +   L YL   +   L  K F Q + E  K+FT      FV+K      V LL  + +    F      ++ L  +F  D+ ++L   CP + EVF  F
Sbjct:    1 MALSFTARLFINNRVLRTERILQSLPKC--NVYESAKKYVNAVKKINLENTLTGSSELVQFPFKSFYKFPVDCLKTMIEESLNILKEKGKAKNIIVYNQMMFSLWSNKIEFDVDLFLHDIVENKLRINSETFDMIIKCYSREKRIDDIEEVLKKMEKENIQLLRKTLPDVVYAYTVTGQEEKGKEFMKANQVDEDMLSIKVAKMRAYAAIGD----TTKLFQLINSLPEINKGFILSDLYRIMSKTNASDFSLDALIGMMKEDVLVETIKL------MNFEKFYNKGLRILEQNIENISDKLIYRSLMTFDTPTCPPHIYKNVLDLTLSHIFRVRTNSNVFTFMSFDDALSYSLKASSAATNR---ILSFSLLNKAVETKSFDNIHEALKFGQTALTQRMWHYMIQTVLIDKTLITKLVKDSTELMSIFTHSQYGLLPDVILYHICSGDFSEVTRLEASFPDQVFHRYATGLNYLVHRLVTVLGVKNFPQVKQEISKFFTPA----FVNKLFSMTFVKLLVNDSECDASFEDDLKSIKELQVKFMPDNISYLCRTCPSRAEVFNSF 547
The following BLAST results are available for this feature:
BLAST of SMED30015220 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015220 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015220 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015220 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015220 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015220 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015220 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015220 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015220 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015220 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
lrpprc2.236e-726.19leucine-rich pentatricopeptide repeat containing [... [more]
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BLAST of SMED30015220 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015220 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015220 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015220 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000027150.10.000e+0100.00SMESG000027150.1[more]
SMESG000027150.10.000e+0100.00SMESG000027150.1[more]
SMESG000026537.13.670e-3927.39SMESG000026537.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015220 ID=SMED30015220|Name=SMED30015220|organism=Schmidtea mediterranea sexual|type=transcript|length=1926bp
TTAAATATATAGGTTTTGTGTTTGATTTTAAAACAACACGTTTGTTGCAT
TTATTTTTTTAATTATTATCTAATTAATACAATGGCATTTTTTGCGACAA
TCCGACTTATGATAGTTTCAAAAAGTTTAACAGAACCAATAATAAGAAAT
TTACTCAAATTCACACCGAATGTATCCTCCTTAAAAGATGAATACGTTCT
ATATTTAAAAAATCTTAATATCCCTCTTAATAAACACAATGCGTTTGAAC
TATTAGAATTTCCTTACAAATCTTTTCATATGTTTTCTGACATTGACATG
AAAAAAATTATAGATGAATCATTTAAAATTATTAAAGAACAAGATCTTGC
TAACTCAGTGTCGGTTTATAATGCTCTAATTAATTCACAGGTTGATTTCG
ATATAGATTTTGATATCGATACCATTCTTAGTGAAATTTCCAAGAAAAAA
ATTACTTTCAATCAAGATACGTATAAAATTCTTATTAAATATTATGCTAA
GCGTGGTAATTTGGATGCTATTGAATTAATAGTCAGTAAAATGAAATCTA
TGAATCTCACTGTATCCAATAAGCAACTGATGATAATTCTTTCTGCATAT
TACGTGAACGGACTGCAAGAAAAAGCACAGATGTTTATCAAAGAAAATAA
TATGGATGAAAATGGGATTTATGTGACGCTTTCGAAAGCAAAGAGCTATG
CGACACTTGGTGATGTGAATAATGCAACAAAACATTTGTTTGAGATTTAC
AAAAACGATCTCTCATTTTCGTCGAAATATTGTTTCACTATTTATTCGCT
TTTAGTTGCAGCCGGCACTCCTAAACCCCAATTAGAGAAAATTATAAAAG
TAATGAAGAAGATGATTGTCAAAAAACCAATTTATATCCACTTTGCAATC
CAAAAATTGAATAAAGCTAAGTGTTTTGATCAATCTCTGATTTTTCTAAA
GGATTTTCTAAATTTTGTTGACGAAACAACGGCGTTCTACCTGATCGAGC
TGTTCACTATACCAGCTTGTCCTAAAAAGGTCTACAAAAATGTCTTAAAA
CTTTTATTATGCCGCATTGGAACGACTAATAATGTTTTCAATGCATTCTC
CAACATGGAATTCGAGGAAGCAATGGAGTTCGCGGTAGCAGAAAGCAATG
AAAGGAACATAGAGTGCATCTGTAAATCCCTCATTAATAAAGCACTCCGA
GAGAAATCATTGGAAAATATTGAGGCGTCCATAAATTTTACAATGCATAA
TATGTTTTGGACAAGAGTCAATTTAATTCTCCAGCACATGGTGCTGCCGT
TTGCGGTGGAATTGAAAATTGTGTCGAACCATGAGGAAATATTGAGTTTA
TTTCCACAATTTAATAATATTTTCCTGTCAGTTATATTGATGAATTACCT
GACCTATGGTAAATTCAAGGAATACGATACGTTGAGAAAGAAATATCTCC
ATCTCACAAATGTCCCCTTCATGGCTGAGCTCGGTTATTTGTGTAAAACT
ATCAGCGACAATTTATCTCCAAAAGAATTCGAGCAATTTGAATCCGAAGG
GAAAAAATATTTTACGGAAAAATGTATTCGGAATTTCGTTCACAAAGCTT
CGTTTTATAGAATTGTCAATTTACTAAAAACGAATCAAGATGCCAAGGAA
ATGTTTTCATTATTGGAAAGATTGAATTATCAATTTCAGGAAGATGACTT
CAATTTTTTGAAAATCAAATGTCCGGATAAAATAGAAGTATTCAAGCCGT
TTTTCATCAATACAATTCAAGTGACCAACTCCTAGGAAATTTGATAATAG
GCACATTTTAATTAATTCGCAGCTTTTAGAGTTTTTGGTAATTATATAGC
AACTGTTCTATCATTTGATTCTTGAGTTTTATCTAGACTAATTTGCTTAT
TCAATTATTATTAATATTATTATTAT
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protein sequence of SMED30015220-orf-1

>SMED30015220-orf-1 ID=SMED30015220-orf-1|Name=SMED30015220-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=568bp
MAFFATIRLMIVSKSLTEPIIRNLLKFTPNVSSLKDEYVLYLKNLNIPLN
KHNAFELLEFPYKSFHMFSDIDMKKIIDESFKIIKEQDLANSVSVYNALI
NSQVDFDIDFDIDTILSEISKKKITFNQDTYKILIKYYAKRGNLDAIELI
VSKMKSMNLTVSNKQLMIILSAYYVNGLQEKAQMFIKENNMDENGIYVTL
SKAKSYATLGDVNNATKHLFEIYKNDLSFSSKYCFTIYSLLVAAGTPKPQ
LEKIIKVMKKMIVKKPIYIHFAIQKLNKAKCFDQSLIFLKDFLNFVDETT
AFYLIELFTIPACPKKVYKNVLKLLLCRIGTTNNVFNAFSNMEFEEAMEF
AVAESNERNIECICKSLINKALREKSLENIEASINFTMHNMFWTRVNLIL
QHMVLPFAVELKIVSNHEEILSLFPQFNNIFLSVILMNYLTYGKFKEYDT
LRKKYLHLTNVPFMAELGYLCKTISDNLSPKEFEQFESEGKKYFTEKCIR
NFVHKASFYRIVNLLKTNQDAKEMFSLLERLNYQFQEDDFNFLKIKCPDK
IEVFKPFFINTIQVTNS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR011990TPR-like_helical_dom_sf
IPR002885Pentatricopeptide_repeat
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0003723RNA binding
GO:0004519endonuclease activity
Vocabulary: biological process
TermDefinition
GO:0009451RNA modification
GO:0090305nucleic acid phosphodiester bond hydrolysis
Vocabulary: cellular component
TermDefinition
GO:0043231intracellular membrane-bounded organelle
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3DG3DSA:1.25.40.10coord: 72..205
e-value: 3.0E-10
score: 42.0
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 130..160
e-value: 0.0026
score: 15.8
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 127..161
score: 8.879