Calcyphosin protein

Overview
NameCalcyphosin protein
Smed IDSMED30015209
Length (bp)1412
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Calcyphosin protein (SMED30015209) t-SNE clustered cells

Violin plots show distribution of expression levels for Calcyphosin protein (SMED30015209) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Calcyphosin protein (SMED30015209) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Calcyphosin protein (SMED30015209) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30015209

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30015209SMESG000005291.1 dd_Smed_v4_7209_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30015209SMESG000005291.1 dd_Smed_v4_7209_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30015209SMESG000005291.1 dd_Smed_v4_7209_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30015209SMESG000005291.1 dd_Smed_v4_7209_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30015209SMESG000005291.1 dd_Smed_v6_7209_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
epidermisSMED30015209SMESG000005291.1 dd_Smed_v4_7209_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Calcyphosin protein vs. Ensembl Human
Match: CAPSL (calcyphosine like [Source:HGNC Symbol;Acc:HGNC:28375])

HSP 1 Score: 268.085 bits (684), Expect = 1.492e-87
Identity = 123/208 (59.13%), Postives = 168/208 (80.77%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            MA T+R ++E+ +Q+ KKL  ATDP+ERLRL CL+RG+ GIKGL R F+I DDD +R+LDF EF KG+ DY +VM+  E+ E+F + D+D +GT+DF+EFL+ LRPPM ++R E+I +AF K+DKT DG IT+EDL+ VY+A HHPK+++GEW+E+QVFR+FL NF++P + DG+VT EEF+NYY G+SASID D YF +MMR A+KL
Sbjct:    1 MAGTARHDREMAIQAKKKLTTATDPIERLRLQCLARGSAGIKGLGRVFRIMDDDNNRTLDFKEFMKGLNDYAVVMEKEEVEELFRRFDKDGNGTIDFNEFLLTLRPPMSRARKEVIMQAFRKLDKTGDGVITIEDLREVYNAKHHPKYQNGEWSEEQVFRKFLDNFDSPYDKDGLVTPEEFMNYYAGVSASIDTDVYFIIMMRTAWKL 208          
BLAST of Calcyphosin protein vs. Ensembl Human
Match: CAPSL (calcyphosine like [Source:HGNC Symbol;Acc:HGNC:28375])

HSP 1 Score: 268.085 bits (684), Expect = 1.492e-87
Identity = 123/208 (59.13%), Postives = 168/208 (80.77%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            MA T+R ++E+ +Q+ KKL  ATDP+ERLRL CL+RG+ GIKGL R F+I DDD +R+LDF EF KG+ DY +VM+  E+ E+F + D+D +GT+DF+EFL+ LRPPM ++R E+I +AF K+DKT DG IT+EDL+ VY+A HHPK+++GEW+E+QVFR+FL NF++P + DG+VT EEF+NYY G+SASID D YF +MMR A+KL
Sbjct:    1 MAGTARHDREMAIQAKKKLTTATDPIERLRLQCLARGSAGIKGLGRVFRIMDDDNNRTLDFKEFMKGLNDYAVVMEKEEVEELFRRFDKDGNGTIDFNEFLLTLRPPMSRARKEVIMQAFRKLDKTGDGVITIEDLREVYNAKHHPKYQNGEWSEEQVFRKFLDNFDSPYDKDGLVTPEEFMNYYAGVSASIDTDVYFIIMMRTAWKL 208          
BLAST of Calcyphosin protein vs. Ensembl Human
Match: CAPSL (calcyphosine like [Source:HGNC Symbol;Acc:HGNC:28375])

HSP 1 Score: 268.085 bits (684), Expect = 1.492e-87
Identity = 123/208 (59.13%), Postives = 168/208 (80.77%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            MA T+R ++E+ +Q+ KKL  ATDP+ERLRL CL+RG+ GIKGL R F+I DDD +R+LDF EF KG+ DY +VM+  E+ E+F + D+D +GT+DF+EFL+ LRPPM ++R E+I +AF K+DKT DG IT+EDL+ VY+A HHPK+++GEW+E+QVFR+FL NF++P + DG+VT EEF+NYY G+SASID D YF +MMR A+KL
Sbjct:    1 MAGTARHDREMAIQAKKKLTTATDPIERLRLQCLARGSAGIKGLGRVFRIMDDDNNRTLDFKEFMKGLNDYAVVMEKEEVEELFRRFDKDGNGTIDFNEFLLTLRPPMSRARKEVIMQAFRKLDKTGDGVITIEDLREVYNAKHHPKYQNGEWSEEQVFRKFLDNFDSPYDKDGLVTPEEFMNYYAGVSASIDTDVYFIIMMRTAWKL 208          
BLAST of Calcyphosin protein vs. Ensembl Human
Match: CAPSL (calcyphosine like [Source:HGNC Symbol;Acc:HGNC:28375])

HSP 1 Score: 243.047 bits (619), Expect = 3.768e-78
Identity = 111/190 (58.42%), Postives = 154/190 (81.05%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISA 651
            MA T+R ++E+ +Q+ KKL  ATDP+ERLRL CL+RG+ GIKGL R F+I DDD +R+LDF EF KG+ DY +VM+  E+ E+F + D+D +GT+DF+EFL+ LRPPM ++R E+I +AF K+DKT DG IT+EDL+ VY+A HHPK+++GEW+E+QVFR+FL NF++P + DG+VT EEF+NYY G+SA
Sbjct:    1 MAGTARHDREMAIQAKKKLTTATDPIERLRLQCLARGSAGIKGLGRVFRIMDDDNNRTLDFKEFMKGLNDYAVVMEKEEVEELFRRFDKDGNGTIDFNEFLLTLRPPMSRARKEVIMQAFRKLDKTGDGVITIEDLREVYNAKHHPKYQNGEWSEEQVFRKFLDNFDSPYDKDGLVTPEEFMNYYAGVSA 190          
BLAST of Calcyphosin protein vs. Ensembl Human
Match: CAPSL (calcyphosine like [Source:HGNC Symbol;Acc:HGNC:28375])

HSP 1 Score: 222.246 bits (565), Expect = 4.407e-70
Identity = 102/178 (57.30%), Postives = 144/178 (80.90%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTK 615
            MA T+R ++E+ +Q+ KKL  ATDP+ERLRL CL+RG+ GIKGL R F+I DDD +R+LDF EF KG+ DY +VM+  E+ E+F + D+D +GT+DF+EFL+ LRPPM ++R E+I +AF K+DKT DG IT+EDL+ VY+A HHPK+++GEW+E+QVFR+FL NF++P + DG+V+K
Sbjct:    1 MAGTARHDREMAIQAKKKLTTATDPIERLRLQCLARGSAGIKGLGRVFRIMDDDNNRTLDFKEFMKGLNDYAVVMEKEEVEELFRRFDKDGNGTIDFNEFLLTLRPPMSRARKEVIMQAFRKLDKTGDGVITIEDLREVYNAKHHPKYQNGEWSEEQVFRKFLDNFDSPYDKDGLVSK 178          
BLAST of Calcyphosin protein vs. Ensembl Celegans
Match: cal-1 (Calmodulin-like protein [Source:UniProtKB/Swiss-Prot;Acc:P04630])

HSP 1 Score: 55.0694 bits (131), Expect = 7.334e-9
Identity = 42/142 (29.58%), Postives = 72/142 (50.70%), Query Frame = 1
Query:  202 IKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFL 627
            I      F +FD DG+ ++   E    +   G      EI E+ N++D D +G ++F EF + ++  M ++  EMI +AF   DK  +G IT ++ +  Y  VH       +++E++V  E +K  E   + DG +  EEF+
Sbjct:   23 IDEFREAFMMFDKDGNGTISTKELGIAMRSLGQNPTEQEILEMINEVDIDGNGQIEFPEFCVMMKRMMKETDSEMIREAFRVFDKDGNGVITAQEFR--YFMVHM----GMQFSEEEV-DEMIK--EVDVDGDGEIDYEEFV 155          
BLAST of Calcyphosin protein vs. Ensembl Celegans
Match: cal-1 (Calmodulin-like protein [Source:UniProtKB/Swiss-Prot;Acc:P04630])

HSP 1 Score: 55.4546 bits (132), Expect = 8.153e-9
Identity = 41/136 (30.15%), Postives = 71/136 (52.21%), Query Frame = 1
Query:  220 TFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFL 627
             F +FD DG+ ++   E    +   G      EI E+ N++D D +G ++F EF + ++  M ++  EMI +AF   DK  +G IT ++ +  Y  VH       +++E++V  E +K  E   + DG +  EEF+
Sbjct:   48 AFMMFDKDGNGTISTKELGIAMRSLGQNPTEQEILEMINEVDIDGNGQIEFPEFCVMMKRMMKETDSEMIREAFRVFDKDGNGVITAQEFR--YFMVHM----GMQFSEEEV-DEMIK--EVDVDGDGEIDYEEFV 174          
BLAST of Calcyphosin protein vs. Ensembl Celegans
Match: E02A10.3 (pep chromosome:WBcel235:V:12585099:12587991:1 gene:WBGene00008453.1 transcript:E02A10.3a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:E02A10.3)

HSP 1 Score: 55.0694 bits (131), Expect = 1.281e-8
Identity = 35/138 (25.36%), Postives = 75/138 (54.35%), Query Frame = 1
Query:  217 RTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRP-PMPKSRV-EMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEF 624
            + F +FD D S ++  +E +  I + G+    +E+ +I +++D+  +  +DFDEF + +R   M KS   E++ + F   D++++G I+ +D + +   +       G+ T++Q+  E     EA  + +G++  +EF
Sbjct:   60 QVFNMFDADRSGAIAIDELEAAIKNLGLEQTRDELDKIIDEVDQRGNHQIDFDEFCVVMRRLTMKKSNWNEVVKECFTVFDRSENGGISKKDFRFILREL-------GDITDNQIIDEIFN--EADVDGNGVIDYDEF 188          
BLAST of Calcyphosin protein vs. Ensembl Celegans
Match: E02A10.3 (pep chromosome:WBcel235:V:12584830:12587945:1 gene:WBGene00008453.1 transcript:E02A10.3c.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:E02A10.3)

HSP 1 Score: 55.0694 bits (131), Expect = 1.668e-8
Identity = 35/138 (25.36%), Postives = 75/138 (54.35%), Query Frame = 1
Query:  217 RTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRP-PMPKSRV-EMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEF 624
            + F +FD D S ++  +E +  I + G+    +E+ +I +++D+  +  +DFDEF + +R   M KS   E++ + F   D++++G I+ +D + +   +       G+ T++Q+  E     EA  + +G++  +EF
Sbjct:   60 QVFNMFDADRSGAIAIDELEAAIKNLGLEQTRDELDKIIDEVDQRGNHQIDFDEFCVVMRRLTMKKSNWNEVVKECFTVFDRSENGGISKKDFRFILREL-------GDITDNQIIDEIFN--EADVDGNGVIDYDEF 188          
BLAST of Calcyphosin protein vs. Ensembl Celegans
Match: E02A10.3 (pep chromosome:WBcel235:V:12586477:12587945:1 gene:WBGene00008453.1 transcript:E02A10.3f.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:E02A10.3)

HSP 1 Score: 54.299 bits (129), Expect = 2.137e-8
Identity = 35/138 (25.36%), Postives = 75/138 (54.35%), Query Frame = 1
Query:  217 RTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRP-PMPKSRV-EMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEF 624
            + F +FD D S ++  +E +  I + G+    +E+ +I +++D+  +  +DFDEF + +R   M KS   E++ + F   D++++G I+ +D + +   +       G+ T++Q+  E     EA  + +G++  +EF
Sbjct:   53 QVFNMFDADRSGAIAIDELEAAIKNLGLEQTRDELDKIIDEVDQRGNHQIDFDEFCVVMRRLTMKKSNWNEVVKECFTVFDRSENGGISKKDFRFILREL-------GDITDNQIIDEIFN--EADVDGNGVIDYDEF 181          
BLAST of Calcyphosin protein vs. Ensembl Fly
Match: CG31345 (gene:FBgn0051345 transcript:FBtr0082689)

HSP 1 Score: 241.121 bits (614), Expect = 2.913e-77
Identity = 121/203 (59.61%), Postives = 150/203 (73.89%), Query Frame = 1
Query:  109 ELQVQSNKKLQNA--TDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFE--APEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            EL+  + ++L N    DP+ +LRL C SRGATGI GLSR+F++ DDDGS+SL   EFKKG+ D G+ + ++EI E+F++ D D SG ++  EFL+ LRPPM  SR+ +I KAF KMD   DG ITV DLK VYS   HPK+ SGE TE+Q+F +FLKNFE  AP   DGIVT+EEF+NYY  ISASIDNDAYFDLMMR AYKL
Sbjct:   12 ELKNLAKRELANGLDKDPIYKLRLQCFSRGATGILGLSRSFRVMDDDGSKSLSPEEFKKGVTDIGLDLTDSEIDEMFSRFDTDGSGNINMTEFLVKLRPPMNNSRISIIEKAFDKMDANGDGQITVTDLKNVYSVRDHPKYLSGEMTENQIFTQFLKNFEVGAPNP-DGIVTREEFINYYATISASIDNDAYFDLMMRQAYKL 213          
BLAST of Calcyphosin protein vs. Ensembl Fly
Match: CG10126 (gene:FBgn0038088 transcript:FBtr0082686)

HSP 1 Score: 220.705 bits (561), Expect = 2.623e-69
Identity = 102/184 (55.43%), Postives = 136/184 (73.91%), Query Frame = 1
Query:  151 DPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPE-EIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAY 699
            DP+ +LRL+CLSRGATGI GL R F+  DDDGS++L+  EF  GI D G+ +   EI ++F   D D SG+++  EFL+ LRPPMP+SR+ +I +AF KMD+ +DG IT++DLK VYS   HPK++SGE +ED++  +FL NFE     +DG +T+EEF+NYY  ISASIDND +FDLMMR AY
Sbjct:   30 DPITKLRLLCLSRGATGILGLGRAFRAMDDDGSKALNEEEFITGIRDTGLDVSEEEIKQMFATFDEDGSGSINMTEFLLKLRPPMPQSRLNIIDQAFNKMDRDEDGVITIQDLKNVYSVKEHPKYQSGEKSEDEILTDFLHNFEGGRGNLDGKITREEFVNYYATISASIDNDMFFDLMMRRAY 213          
BLAST of Calcyphosin protein vs. Ensembl Fly
Match: CG10126 (gene:FBgn0038088 transcript:FBtr0082687)

HSP 1 Score: 219.935 bits (559), Expect = 7.510e-69
Identity = 102/184 (55.43%), Postives = 136/184 (73.91%), Query Frame = 1
Query:  151 DPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPE-EIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAY 699
            DP+ +LRL+CLSRGATGI GL R F+  DDDGS++L+  EF  GI D G+ +   EI ++F   D D SG+++  EFL+ LRPPMP+SR+ +I +AF KMD+ +DG IT++DLK VYS   HPK++SGE +ED++  +FL NFE     +DG +T+EEF+NYY  ISASIDND +FDLMMR AY
Sbjct:   42 DPITKLRLLCLSRGATGILGLGRAFRAMDDDGSKALNEEEFITGIRDTGLDVSEEEIKQMFATFDEDGSGSINMTEFLLKLRPPMPQSRLNIIDQAFNKMDRDEDGVITIQDLKNVYSVKEHPKYQSGEKSEDEILTDFLHNFEGGRGNLDGKITREEFVNYYATISASIDNDMFFDLMMRRAY 225          
BLAST of Calcyphosin protein vs. Ensembl Fly
Match: CG31802 (gene:FBgn0051802 transcript:FBtr0081058)

HSP 1 Score: 53.5286 bits (127), Expect = 4.997e-8
Identity = 42/142 (29.58%), Postives = 70/142 (49.30%), Query Frame = 1
Query:  211 LSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMP-KSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNY 633
            + + F +FD   +  ++  E +  I   G      +I  + ++ID+DK+G + F++FL  +R  M  K   + + KAF+  D  + G I+  +LK V   +       GE   D+  +E +   EA    DG V+KEEFLN 
Sbjct:   47 IKKAFDLFDTQCTGFIETKELRVAIRALGFEPKKEDIKRMMDEIDKDKTGRIAFNDFLYLMRLKMAEKDSNQDMMKAFSFFDDDRTGGISFLNLKRVAKEL-------GEQLTDEELQEMID--EANVSGDGEVSKEEFLNL 179          
BLAST of Calcyphosin protein vs. Ensembl Fly
Match: CG17493 (gene:FBgn0040010 transcript:FBtr0332630)

HSP 1 Score: 52.373 bits (124), Expect = 1.199e-7
Identity = 45/140 (32.14%), Postives = 65/140 (46.43%), Query Frame = 1
Query:  211 LSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMP-KSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFL 627
            +   F +FD++G+  ++  E K  I   G      EI  + + ID+D SG + F+ FL  +   M  K   E I KAF   D    G I+  +LK V   +       GE   D+  RE +   EA  + DG V +EEFL
Sbjct:   43 IKEAFDLFDNEGTGYIEVKELKVAIRALGFEPKKEEIKRMISDIDKDCSGRIAFNVFLQLMTIKMAEKDTKEEILKAFRLFDDDDTGKISFRNLKRVAREL-------GETLTDEELREMID--EADLDNDGEVNQEEFL 173          
BLAST of Calcyphosin protein vs. Ensembl Zebrafish
Match: capslb (calcyphosine-like b [Source:ZFIN;Acc:ZDB-GENE-050417-298])

HSP 1 Score: 279.641 bits (714), Expect = 1.102e-91
Identity = 127/210 (60.48%), Postives = 162/210 (77.14%), Query Frame = 1
Query:   76 FTMAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            + MA TSR N+E+ + + K+L   +DP+ERLRL CLSRG  GIKGL RTF+I DDD +R+LD  EF KG+ DYG++M+  E   +F Q DRD SG +DFDEFLI LRPPM  +R E++ +AF K+DKT DG ITVEDLKGVY    HPK+ +GEWTEDQ+FR+FL +F++P++ DG VTKEEF+NYY G+SASID D YF LMMRNA++L
Sbjct:   38 WRMAGTSRHNREMMINAKKQLAECSDPIERLRLQCLSRGCAGIKGLGRTFRIMDDDNNRTLDLKEFIKGLNDYGVLMEKEEAINLFQQFDRDGSGQIDFDEFLITLRPPMSNARKEVVLQAFKKLDKTGDGVITVEDLKGVYDVRKHPKYLNGEWTEDQIFRKFLDSFDSPDDKDGKVTKEEFMNYYSGVSASIDTDIYFILMMRNAWRL 247          
BLAST of Calcyphosin protein vs. Ensembl Zebrafish
Match: capsla (calcyphosine-like a [Source:ZFIN;Acc:ZDB-GENE-030131-7291])

HSP 1 Score: 239.58 bits (610), Expect = 4.899e-77
Identity = 106/187 (56.68%), Postives = 144/187 (77.01%), Query Frame = 1
Query:  145 ATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            A D ++ LR  CLSRGA GIKGL R F+  DDDGS+SLDF EF +G+ DYG+ +  ++  +IF  +D+D SG+++FDEFL  LRPPM  +R+++I +AF K DK+ DG +TVEDL+GVY++ HHPK+KSGEWTE QVF  FL +F++P + DG VT EEF+NYY G+SAS+D+D YF  MM++A+KL
Sbjct:    2 AADALQDLRQQCLSRGAAGIKGLGRMFRSMDDDGSKSLDFQEFVRGLQDYGVSVGRDQAQQIFAMMDKDGSGSINFDEFLEKLRPPMSSARMQVIRQAFQKFDKSGDGVVTVEDLQGVYNSKHHPKYKSGEWTETQVFHSFLDSFDSPHDKDGKVTLEEFVNYYSGVSASVDSDEYFISMMKSAWKL 188          
BLAST of Calcyphosin protein vs. Ensembl Zebrafish
Match: kcnip2 (Kv channel interacting protein 2 [Source:ZFIN;Acc:ZDB-GENE-081031-91])

HSP 1 Score: 51.9878 bits (123), Expect = 9.869e-8
Identity = 31/105 (29.52%), Postives = 57/105 (54.29%), Query Frame = 1
Query:  322 HEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFK-SGEWTEDQVFREFLKNF--EAPEEIDGIVTKEEFL 627
            H +F   DR K+G ++F++F++ L   +  +  + +S AF+  D  KDG IT E++  +  +++    K +     D   RE +++F  +     DG++T EEFL
Sbjct:   14 HFLFEAFDRRKNGAVNFEDFVVGLSIILRGTVTDRLSWAFSLYDLNKDGCITKEEMNDIMKSIYDMMGKNTCPCMNDSAPREHVESFFQKMDRNNDGVITMEEFL 118          
BLAST of Calcyphosin protein vs. Ensembl Zebrafish
Match: cetn4 (centrin 4 [Source:ZFIN;Acc:ZDB-GENE-030826-21])

HSP 1 Score: 52.7582 bits (125), Expect = 1.083e-7
Identity = 43/140 (30.71%), Postives = 66/140 (47.14%), Query Frame = 1
Query:  211 LSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMP-KSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFL 627
            +   F +FD DGS ++D  E K  +   G      EI ++   ID++ SG + F +FL  +   M  K   E I KAF   D    G I+ ++LK V   +       GE   D+  +E +   EA  + DG + ++EFL
Sbjct:   32 IKEAFDLFDTDGSGTIDVKELKVAMRALGFEPKKEEIKKMIADIDKEGSGVIGFSDFLSMMTQKMSEKDSKEEILKAFRLFDDDCTGKISFKNLKRVAKEL-------GENLTDEELQEMID--EADRDGDGEINEQEFL 162          
BLAST of Calcyphosin protein vs. Ensembl Zebrafish
Match: si:ch211-145b13.5 (si:ch211-145b13.5 [Source:ZFIN;Acc:ZDB-GENE-090313-35])

HSP 1 Score: 52.7582 bits (125), Expect = 2.132e-7
Identity = 34/108 (31.48%), Postives = 58/108 (53.70%), Query Frame = 1
Query:  328 IFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVH-------HPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLN 630
            +FN  D+DK+G+L F+E +  L   +  +  E ++ AF   D  KDG IT E++  +  +++       HP+ K  E    Q  R F +N +  +  DG+VT +EF++
Sbjct:  103 LFNAFDKDKNGSLSFEELVCDLSVLLRGTTEEKLNWAFNLYDINKDGQITKEEMLDIMKSIYDLMGKSIHPRLK--EEAVRQHVRMFFQNMDINQ--DGVVTIDEFID 206          
BLAST of Calcyphosin protein vs. Ensembl Xenopus
Match: capsl (calcyphosine like [Source:NCBI gene;Acc:549278])

HSP 1 Score: 288.886 bits (738), Expect = 1.063e-95
Identity = 127/208 (61.06%), Postives = 169/208 (81.25%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            M  T+R ++++ +Q+ K L + TDPVE+LRL CLSRG+ GIKGL R F+I DDDG+++LDF EF KG++DYG++MD  E  E+F+  D+D SGT+DFDEFL+ LRPPM  +R +MI +AF K+DK+ DG +T+EDL+GVY+A HHPK+++GEWTEDQVFR FL NF++P + DG VTK+EFLNYY G+SASID+D YF LMMRNA+KL
Sbjct:    1 MPGTARHDRDMALQAKKNLSSCTDPVEKLRLQCLSRGSAGIKGLGRVFRIMDDDGNKTLDFKEFSKGLSDYGVMMDKTETQELFSVFDKDGSGTIDFDEFLVTLRPPMSNARKDMILQAFRKLDKSGDGVVTIEDLRGVYNAKHHPKYQNGEWTEDQVFRSFLDNFDSPYDKDGQVTKDEFLNYYSGVSASIDSDTYFILMMRNAWKL 208          
BLAST of Calcyphosin protein vs. Ensembl Xenopus
Match: capsl (calcyphosine like [Source:NCBI gene;Acc:549278])

HSP 1 Score: 283.108 bits (723), Expect = 1.590e-93
Identity = 124/208 (59.62%), Postives = 166/208 (79.81%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            M  T+R ++++ +Q+ K L + TDPVE+LRL CLSRG+ GIKGL R F+I DDDG+++LDF EF KG++DYG++MD  E  E+F+  D+D SGT+DFDEFL+ LRPPM  +R +MI +AF K+DK+ DG +T+EDL+GVY+A HHPK+++GEWTEDQVFR FL NF++P + DG VT +EF+NYY G+SASID D YF  MMRNA+KL
Sbjct:    1 MPGTARHDRDMALQAKKNLSSCTDPVEKLRLQCLSRGSAGIKGLGRVFRIMDDDGNKTLDFKEFSKGLSDYGVMMDKTETQELFSVFDKDGSGTIDFDEFLVTLRPPMSNARKDMILQAFRKLDKSGDGVVTIEDLRGVYNAKHHPKYQNGEWTEDQVFRSFLDNFDSPYDKDGQVTPDEFMNYYAGVSASIDTDIYFITMMRNAWKL 208          
BLAST of Calcyphosin protein vs. Ensembl Xenopus
Match: capsl (calcyphosine like [Source:NCBI gene;Acc:549278])

HSP 1 Score: 276.174 bits (705), Expect = 6.603e-91
Identity = 121/198 (61.11%), Postives = 159/198 (80.30%), Query Frame = 1
Query:  112 LQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            + +Q+ K L + TDPVE+LRL CLSRG+ GIKGL R F+I DDDG+++LDF EF KG++DYG++MD  E  E+F+  D+D SGT+DFDEFL+ LRPPM  +R +MI +AF K+DK+ DG +T+EDL+GVY+A HHPK+++GEWTEDQVFR FL NF++P + DG VT +EF+NYY G+SASID D YF  MMRNA+KL
Sbjct:    1 MALQAKKNLSSCTDPVEKLRLQCLSRGSAGIKGLGRVFRIMDDDGNKTLDFKEFSKGLSDYGVMMDKTETQELFSVFDKDGSGTIDFDEFLVTLRPPMSNARKDMILQAFRKLDKSGDGVVTIEDLRGVYNAKHHPKYQNGEWTEDQVFRSFLDNFDSPYDKDGQVTPDEFMNYYAGVSASIDTDIYFITMMRNAWKL 198          
BLAST of Calcyphosin protein vs. Ensembl Xenopus
Match: caps (calcyphosine [Source:NCBI gene;Acc:100037881])

HSP 1 Score: 243.047 bits (619), Expect = 8.061e-78
Identity = 109/208 (52.40%), Postives = 156/208 (75.00%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            MA  ++  +E+  ++ ++     DPVE+LRL CL+RGA+GIKGL R F+I DD+ S +LD  EF KG+ ++GI +  +E+ +IF+Q D + +GT++FDEFLI++RPPM  +R ++I  AF KMD+T DG IT+EDLKGVY+   H K+++GEW E QVF+ FL NF++P   DG VT+EEFLNYY G+SASID+DAYF +MM+N +K+
Sbjct:    1 MASMTQLEREMMAKAQRQYPQCQDPVEKLRLQCLARGASGIKGLGRAFRIMDDNRSSTLDLEEFSKGLQNFGISLQPSEVLDIFHQFDTNGNGTINFDEFLISIRPPMSNARRQVILDAFKKMDRTGDGVITIEDLKGVYNPKFHQKYRNGEWNERQVFQNFLDNFDSPNNKDGQVTEEEFLNYYSGVSASIDSDAYFVVMMKNEWKI 208          
BLAST of Calcyphosin protein vs. Ensembl Xenopus
Match: ENSXETT00000016513.1 (calcyphosine 2 [Source:NCBI gene;Acc:100380015])

HSP 1 Score: 85.5001 bits (210), Expect = 7.353e-19
Identity = 56/173 (32.37%), Postives = 92/173 (53.18%), Query Frame = 1
Query:  184 SRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQI-DRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAY 699
            SRG   + GL + F+  D++G   L   +FK+ +  + + + + EI E F +I D    G LD+ +F  AL   M + R   + KAF K+D  K G I + D++  Y A  HP+  SG  +E+++   FLK  +A       V+  EF ++Y G+S  I +D  F  ++RN++
Sbjct:   11 SRGVQTLTGLGKFFRQVDENGDGILQKAKFKQALKVFHLDV-SEEIFESFWKILDEKHEGKLDYGKFTRALIGEMNEYRKTFVQKAFMKLDPNKSGIIPMIDIRKFYCARKHPQVLSGHSSEEEILSSFLKTLQAACRNPKEVSYCEFEDFYEGLSIEILSDNDFINILRNSW 182          
BLAST of Calcyphosin protein vs. Ensembl Mouse
Match: Capsl (calcyphosine-like [Source:MGI Symbol;Acc:MGI:1922818])

HSP 1 Score: 282.722 bits (722), Expect = 1.798e-93
Identity = 126/208 (60.58%), Postives = 166/208 (79.81%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            MA T+R ++E+ +QS KKL  ATDP+ERLRL CL+RG+ GIKGL R F+I DD+ +R+LDF EF KG+ DY +VM+  E  E+F + DRD SGT+DF+EFL+ LRPPM ++R E+I KAF K+DKT DG IT+EDL+ VY+A HHPK+++GEWTE+QVFR+FL NF++P + DG+VT EEF+NYY G+SASID D YF +MM  A+KL
Sbjct:    1 MAGTARHDREMAIQSKKKLSTATDPIERLRLQCLARGSAGIKGLGRVFRIMDDNNNRTLDFKEFLKGLNDYAVVMEKEEAEELFQRFDRDGSGTIDFNEFLLTLRPPMSRARKEVIMKAFRKLDKTGDGVITIEDLREVYNAKHHPKYQNGEWTEEQVFRKFLDNFDSPYDKDGLVTPEEFMNYYAGVSASIDTDVYFIIMMTTAWKL 208          
BLAST of Calcyphosin protein vs. Ensembl Mouse
Match: Capsl (calcyphosine-like [Source:MGI Symbol;Acc:MGI:1922818])

HSP 1 Score: 138.272 bits (347), Expect = 1.119e-38
Identity = 61/105 (58.10%), Postives = 81/105 (77.14%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALR 396
            MA T+R ++E+ +QS KKL  ATDP+ERLRL CL+RG+ GIKGL R F+I DD+ +R+LDF EF KG+ DY +VM+  E  E+F + DRD SGT+DF+EFL+ LR
Sbjct:    1 MAGTARHDREMAIQSKKKLSTATDPIERLRLQCLARGSAGIKGLGRVFRIMDDNNNRTLDFKEFLKGLNDYAVVMEKEEAEELFQRFDRDGSGTIDFNEFLLTLR 105          
BLAST of Calcyphosin protein vs. Ensembl Mouse
Match: Caps2 (calcyphosphine 2 [Source:MGI Symbol;Acc:MGI:2441980])

HSP 1 Score: 68.5514 bits (166), Expect = 6.861e-12
Identity = 47/185 (25.41%), Postives = 91/185 (49.19%), Query Frame = 1
Query:  160 ERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHE---IFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            ++L+     +GA  + GL R F+  D +G+  L+  +F++ +  + + +   +      I       K+  +D+ EF  A+   M + R   + KAF ++D  K G ++V D++  Y A  HP+  SG  TE+++   FL+  +        V+  EF +YY G+S  +  D  F  ++R  +++
Sbjct:  409 DKLKEQLHKKGARILTGLGRYFQGLDKEGNGLLEKADFQQALKTFHLEVSEQDFESFWLILQGYGHSKN-KVDYGEFKRAIFGEMNEYRKSFVRKAFMQLDFNKTGIVSVIDIRKCYCAKKHPRVISGHSTEEEIKSSFLETLKGTCSKCDEVSYGEFEDYYEGLSIGVAGDEDFVNILRIPWRI 592          
BLAST of Calcyphosin protein vs. Ensembl Mouse
Match: Caps2 (calcyphosphine 2 [Source:MGI Symbol;Acc:MGI:2441980])

HSP 1 Score: 68.5514 bits (166), Expect = 6.998e-12
Identity = 47/185 (25.41%), Postives = 91/185 (49.19%), Query Frame = 1
Query:  160 ERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHE---IFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            ++L+     +GA  + GL R F+  D +G+  L+  +F++ +  + + +   +      I       K+  +D+ EF  A+   M + R   + KAF ++D  K G ++V D++  Y A  HP+  SG  TE+++   FL+  +        V+  EF +YY G+S  +  D  F  ++R  +++
Sbjct:  367 DKLKEQLHKKGARILTGLGRYFQGLDKEGNGLLEKADFQQALKTFHLEVSEQDFESFWLILQGYGHSKN-KVDYGEFKRAIFGEMNEYRKSFVRKAFMQLDFNKTGIVSVIDIRKCYCAKKHPRVISGHSTEEEIKSSFLETLKGTCSKCDEVSYGEFEDYYEGLSIGVAGDEDFVNILRIPWRI 550          
BLAST of Calcyphosin protein vs. Ensembl Mouse
Match: Cetn1 (centrin 1 [Source:MGI Symbol;Acc:MGI:1347086])

HSP 1 Score: 56.6102 bits (135), Expect = 5.033e-9
Identity = 42/136 (30.88%), Postives = 70/136 (51.47%), Query Frame = 1
Query:  223 FKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMP-KSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFL 627
            F +FD DGS ++D  E K  +   G      E+ ++ +++D++ +G + F++FL  +   M  K   E I KAF   D  + G I+ ++LK V + +       GE   D+  +E +   EA  + DG V +EEFL
Sbjct:   37 FDLFDSDGSGTIDVKELKVAMRALGFEPRKEEMKKMISEVDKEATGKISFNDFLAVMTQKMAEKDTKEEILKAFRLFDDDETGKISFKNLKRVANEL-------GESLTDEELQEMID--EADRDGDGEVNEEEFL 163          
BLAST of Calcyphosin protein vs. UniProt/SwissProt
Match: sp|Q6P8Y1|CAPSL_MOUSE (Calcyphosin-like protein OS=Mus musculus OX=10090 GN=Capsl PE=2 SV=4)

HSP 1 Score: 282.722 bits (722), Expect = 1.258e-92
Identity = 126/208 (60.58%), Postives = 166/208 (79.81%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            MA T+R ++E+ +QS KKL  ATDP+ERLRL CL+RG+ GIKGL R F+I DD+ +R+LDF EF KG+ DY +VM+  E  E+F + DRD SGT+DF+EFL+ LRPPM ++R E+I KAF K+DKT DG IT+EDL+ VY+A HHPK+++GEWTE+QVFR+FL NF++P + DG+VT EEF+NYY G+SASID D YF +MM  A+KL
Sbjct:    1 MAGTARHDREMAIQSKKKLSTATDPIERLRLQCLARGSAGIKGLGRVFRIMDDNNNRTLDFKEFLKGLNDYAVVMEKEEAEELFQRFDRDGSGTIDFNEFLLTLRPPMSRARKEVIMKAFRKLDKTGDGVITIEDLREVYNAKHHPKYQNGEWTEEQVFRKFLDNFDSPYDKDGLVTPEEFMNYYAGVSASIDTDVYFIIMMTTAWKL 208          
BLAST of Calcyphosin protein vs. UniProt/SwissProt
Match: sp|Q8WWF8|CAPSL_HUMAN (Calcyphosin-like protein OS=Homo sapiens OX=9606 GN=CAPSL PE=2 SV=4)

HSP 1 Score: 268.085 bits (684), Expect = 7.166e-87
Identity = 123/208 (59.13%), Postives = 168/208 (80.77%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            MA T+R ++E+ +Q+ KKL  ATDP+ERLRL CL+RG+ GIKGL R F+I DDD +R+LDF EF KG+ DY +VM+  E+ E+F + D+D +GT+DF+EFL+ LRPPM ++R E+I +AF K+DKT DG IT+EDL+ VY+A HHPK+++GEW+E+QVFR+FL NF++P + DG+VT EEF+NYY G+SASID D YF +MMR A+KL
Sbjct:    1 MAGTARHDREMAIQAKKKLTTATDPIERLRLQCLARGSAGIKGLGRVFRIMDDDNNRTLDFKEFMKGLNDYAVVMEKEEVEELFRRFDKDGNGTIDFNEFLLTLRPPMSRARKEVIMQAFRKLDKTGDGVITIEDLREVYNAKHHPKYQNGEWSEEQVFRKFLDNFDSPYDKDGLVTPEEFMNYYAGVSASIDTDVYFIIMMRTAWKL 208          
BLAST of Calcyphosin protein vs. UniProt/SwissProt
Match: sp|Q0VCC0|CAYP1_BOVIN (Calcyphosin OS=Bos taurus OX=9913 GN=CAPS PE=2 SV=1)

HSP 1 Score: 217.624 bits (553), Expect = 1.704e-67
Identity = 98/183 (53.55%), Postives = 137/183 (74.86%), Query Frame = 1
Query:  157 VERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            +E+LR  CLSRGA+GI+G++R F+  D DGSRSLD  E ++G+A+ G+V+D  E+  +  + DRD SGTLD +EFL ALRPPM ++R  +++ AFAK+D++ DG +TV+DL+GVYS   HPK +SGEWTE+QV R FL NF++ E+ DG VT  EF +YY G+SAS+D D  F  MM +A++L
Sbjct:    8 MEKLRAQCLSRGASGIQGVARFFRRLDQDGSRSLDVRELQRGLAELGLVLDTAEMEGVCRRWDRDGSGTLDLEEFLRALRPPMSQAREAVVTAAFAKLDRSGDGVVTVDDLRGVYSGRTHPKVRSGEWTEEQVLRHFLDNFDSSEK-DGQVTLAEFQDYYSGVSASMDTDEEFVAMMTSAWRL 189          
BLAST of Calcyphosin protein vs. UniProt/SwissProt
Match: sp|Q13938|CAYP1_HUMAN (Calcyphosin OS=Homo sapiens OX=9606 GN=CAPS PE=1 SV=2)

HSP 1 Score: 215.312 bits (547), Expect = 1.746e-65
Identity = 99/183 (54.10%), Postives = 137/183 (74.86%), Query Frame = 1
Query:  157 VERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            +E+LR  CLSRGA+GI+GL+R F+  D DGSRSLD +EF++G+A  G+V+D  E   +  + DR+ SGTLD +EFL ALRPPM ++R  +I+ AFAK+D++ DG +TV+DL+GVYS   HPK +SGEWTED+V R FL NF++ E+ DG VT  EF +YY G+SAS++ D  F  MM +A++L
Sbjct:   94 MEKLRAQCLSRGASGIQGLARFFRQLDRDGSRSLDADEFRQGLAKLGLVLDQAEAEGVCRKWDRNGSGTLDLEEFLRALRPPMSQAREAVIAAAFAKLDRSGDGVVTVDDLRGVYSGRAHPKVRSGEWTEDEVLRRFLDNFDSSEK-DGQVTLAEFQDYYSGVSASMNTDEEFVAMMTSAWQL 275          
BLAST of Calcyphosin protein vs. UniProt/SwissProt
Match: sp|P41150|CAYP1_RABIT (Calcyphosin OS=Oryctolagus cuniculus OX=9986 GN=CAPS PE=1 SV=1)

HSP 1 Score: 211.075 bits (536), Expect = 4.657e-65
Identity = 96/183 (52.46%), Postives = 133/183 (72.68%), Query Frame = 1
Query:  157 VERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            VE+LR  CL+RGA GI+GL+R F+  D DGSRSLD  E ++G+A  G+ +D  E   +  + DR+ SGTLD +EFL ALRPPM ++R  +I+ AFAK+D++ DG +T+ DL+GVYS  +HPK ++GEWTED+V R FL NF++ E+ DG VT  EF +YY G+SAS+D D  F  MM +A++L
Sbjct:    8 VEKLRAQCLARGAAGIQGLARFFRRLDRDGSRSLDAGELRQGLAKLGLALDEAEAQSVCRRWDRNGSGTLDLEEFLRALRPPMSQAREAVIAAAFAKLDRSGDGVVTLGDLRGVYSGRNHPKVRTGEWTEDEVLRRFLDNFDSSEK-DGQVTLAEFQDYYSGLSASVDTDEEFVAMMSSAWQL 189          
BLAST of Calcyphosin protein vs. TrEMBL
Match: A0A267H552 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig007177g1 PE=4 SV=1)

HSP 1 Score: 290.812 bits (743), Expect = 2.421e-93
Identity = 134/208 (64.42%), Postives = 167/208 (80.29%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            M+ T R N+EL  ++  +++ ATDP+ERLRL CL+RGA+GIKGL+R F+I DDDG++ LD  EF KG  D+G V+ + EI  IF+ +D D SG ++FDEFL+ALRPPM ++RVE+I +AF K+DK KDG ITVEDLKGVY    HPK+++GEWTEDQVFREFLK FEAPE  DG+VTKEEFLNYY G+SASIDNDAYF LMMR AY+L
Sbjct:    1 MSATQRLNEELVRKARNQMRTATDPLERLRLTCLARGASGIKGLARQFRIIDDDGNKRLDRKEFHKGCRDFGAVLTDKEIDYIFDHMDADNSGCVNFDEFLVALRPPMSRTRVELIEQAFNKLDKNKDGDITVEDLKGVYCCKFHPKYQNGEWTEDQVFREFLKTFEAPETTDGVVTKEEFLNYYAGVSASIDNDAYFGLMMRTAYRL 208          
BLAST of Calcyphosin protein vs. TrEMBL
Match: A0A564YA36 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS3682 PE=4 SV=1)

HSP 1 Score: 289.271 bits (739), Expect = 9.702e-93
Identity = 132/208 (63.46%), Postives = 168/208 (80.77%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            MA T+R + +L++++ +K+Q++TDPVE+LRL CL+RG +GI GL R F+I DD+G + LD  EF KG  D+GI +   EI E+F  +D+D SG +DFDEFL ALRPPMPK R+E+I++AF KMD+T+DG IT EDLKGVY+   HPK+++GEWTE+QVF EFLK FEAP EIDG VTK EFLNYY G+SASIDN+AYFDLMMRNAYKL
Sbjct:    1 MAITARGDYQLKLKAKQKIQSSTDPVEKLRLNCLARGGSGILGLGRQFRIIDDNGDKKLDMREFTKGCQDFGIDLTKEEIREVFEHLDKDGSGHIDFDEFLEALRPPMPKCRLELINQAFNKMDRTRDGYITAEDLKGVYNCKFHPKYRNGEWTEEQVFNEFLKKFEAPNEIDGKVTKSEFLNYYAGVSASIDNNAYFDLMMRNAYKL 208          
BLAST of Calcyphosin protein vs. TrEMBL
Match: A0A267FSW1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig007177g2 PE=4 SV=1)

HSP 1 Score: 289.271 bits (739), Expect = 9.807e-93
Identity = 133/208 (63.94%), Postives = 167/208 (80.29%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            M+ T R N+EL  ++  +++ ATDP+ERLRL CL+RGA+GIKGL+R F+I DDDG++ LD  EF KG  D+G V+ + EI  IF+ +D D SG ++FDEFL+ALRPPM ++RVE+I +AF K+DK KDG ITVEDLKGVY    HPK+++GEWT+DQVFREFLK FEAPE  DG+VTKEEFLNYY G+SASIDNDAYF LMMR AY+L
Sbjct:    1 MSATQRLNEELVRKARNQMRTATDPLERLRLTCLARGASGIKGLARQFRIIDDDGNKRLDRKEFHKGCRDFGAVLTDKEIDYIFDHMDADNSGCVNFDEFLVALRPPMSRTRVELIEQAFNKLDKNKDGDITVEDLKGVYCCKFHPKYQNGEWTKDQVFREFLKTFEAPETTDGVVTKEEFLNYYAGVSASIDNDAYFGLMMRTAYRL 208          
BLAST of Calcyphosin protein vs. TrEMBL
Match: A0A267H6P0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig002637g1 PE=4 SV=1)

HSP 1 Score: 288.886 bits (738), Expect = 1.348e-92
Identity = 132/210 (62.86%), Postives = 169/210 (80.48%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQN--ATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            MA T R + EL  ++ K+L +  A DP+E+LRL+CLSRGA+GIKG+ R F+I DDDGSRSLD  EF KG  D+G  +   E+ +IF++ID+D+SGTLDFDEFL ALRPPM  +R E+I+KAF K+D+T DG ITVEDL+GVY+  HHPK+++G+WTED VF+EFL+ FE P + DG+VTKEEF NYY G+SAS+DNDAYFDLMMRNAYK+
Sbjct:    1 MAATDRGSAELAAKAKKQLASGKAADPLEKLRLVCLSRGASGIKGIGRQFRIIDDDGSRSLDRREFYKGCTDFGADLSKEEMDQIFDRIDKDRSGTLDFDEFLQALRPPMSSARRELITKAFRKLDRTGDGVITVEDLRGVYNCKHHPKYQNGQWTEDDVFKEFLRTFETPNDADGVVTKEEFFNYYSGVSASVDNDAYFDLMMRNAYKI 210          
BLAST of Calcyphosin protein vs. TrEMBL
Match: F7EF00 (Uncharacterized protein OS=Xenopus tropicalis OX=8364 GN=capsl PE=4 SV=2)

HSP 1 Score: 288.886 bits (738), Expect = 1.668e-92
Identity = 127/208 (61.06%), Postives = 169/208 (81.25%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            M  T+R ++++ +Q+ K L + TDPVE+LRL CLSRG+ GIKGL R F+I DDDG+++LDF EF KG++DYG++MD  E  E+F+  D+D SGT+DFDEFL+ LRPPM  +R +MI +AF K+DK+ DG +T+EDL+GVY+A HHPK+++GEWTEDQVFR FL NF++P + DG VTK+EFLNYY G+SASID+D YF LMMRNA+KL
Sbjct:    1 MPGTARHDRDMALQAKKNLSSCTDPVEKLRLQCLSRGSAGIKGLGRVFRIMDDDGNKTLDFKEFSKGLSDYGVMMDKTETQELFSVFDKDGSGTIDFDEFLVTLRPPMSNARKDMILQAFRKLDKSGDGVVTIEDLRGVYNAKHHPKYQNGEWTEDQVFRSFLDNFDSPYDKDGQVTKDEFLNYYSGVSASIDSDTYFILMMRNAWKL 208          
BLAST of Calcyphosin protein vs. Ensembl Cavefish
Match: capslb (calcyphosine like [Source:NCBI gene;Acc:103021302])

HSP 1 Score: 270.396 bits (690), Expect = 8.104e-89
Identity = 123/208 (59.13%), Postives = 165/208 (79.33%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            MA TSR ++++ + + K+L  ATDPVERLRL CL+RG+ GIKGL R F+I DDD SR+L+  EF KG+ DYGI+M+ +E  E+F   D+D SG +DFDEFLI LRPPM  +R E+I +AF K+DKT DG ITVEDL+GVY+  +HPK+++GEWTEDQ+FR+FL +F++ ++ DG VT+EEF+NYY G+SASID D YF +MM+NA+KL
Sbjct:    1 MAGTSRHDRQMAIDAKKRLSEATDPVERLRLQCLARGSAGIKGLGRVFRIMDDDNSRNLELKEFMKGLNDYGIMMEKDEAMELFRTFDKDGSGQIDFDEFLITLRPPMSNARKEVIMQAFRKLDKTGDGVITVEDLRGVYNVKNHPKYQNGEWTEDQIFRKFLDSFDSDDK-DGKVTQEEFMNYYAGVSASIDTDVYFIVMMKNAWKL 207          
BLAST of Calcyphosin protein vs. Ensembl Cavefish
Match: capslb (calcyphosine like [Source:NCBI gene;Acc:103021302])

HSP 1 Score: 270.011 bits (689), Expect = 1.074e-88
Identity = 124/208 (59.62%), Postives = 165/208 (79.33%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            MA TSR ++++ + + K+L  ATDPVERLRL CL+RG+ GIKGL R F+I DDD SR+L+  EF KG+ DYGI+M+ +E  E+F   D+D SG +DFDEFLI LRPPM  +R E+I +AF K+DKT DG ITVEDL+GVY+  +HPK+++GEWTEDQ+FR+FL +F++ ++ DG VTKEEF+NYY G+SASID+D YF LMM+ A+KL
Sbjct:    1 MAGTSRHDRQMAIDAKKRLSEATDPVERLRLQCLARGSAGIKGLGRVFRIMDDDNSRNLELKEFMKGLNDYGIMMEKDEAMELFRTFDKDGSGQIDFDEFLITLRPPMSNARKEVIMQAFRKLDKTGDGVITVEDLRGVYNVKNHPKYQNGEWTEDQIFRKFLDSFDSDDK-DGKVTKEEFVNYYSGVSASIDSDVYFILMMKTAWKL 207          
BLAST of Calcyphosin protein vs. Ensembl Cavefish
Match: capsla (calcyphosin-like protein [Source:NCBI gene;Acc:103033765])

HSP 1 Score: 249.21 bits (635), Expect = 9.586e-81
Identity = 113/186 (60.75%), Postives = 147/186 (79.03%), Query Frame = 1
Query:  148 TDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
             D +E LR  CLSRGA GIKGL RTF+I DDDGS+SL+  EF+KG+  YG+ +   +  +IF  ID+D+SGT+DF+EFL  LRPPM  +RV++I +AF K+D T DG +T+EDL+GVY++ HHPK+KSGEWTE+QVFR FL +F++P E DG VT EEF+NYY G+SASID+DAYF  MM+NA+KL
Sbjct:    2 ADVLEELRQQCLSRGAAGIKGLGRTFRIMDDDGSKSLNLQEFQKGLETYGVNVGLEKAKQIFTIIDKDRSGTIDFNEFLEKLRPPMSSTRVQVIKQAFQKLDSTGDGLVTIEDLQGVYNSKHHPKYKSGEWTEEQVFRSFLDSFDSPYEKDGKVTLEEFVNYYSGVSASIDDDAYFVTMMQNAWKL 187          
BLAST of Calcyphosin protein vs. Ensembl Cavefish
Match: cetn4 (caltractin [Source:NCBI gene;Acc:103037928])

HSP 1 Score: 56.225 bits (134), Expect = 4.875e-9
Identity = 44/140 (31.43%), Postives = 69/140 (49.29%), Query Frame = 1
Query:  211 LSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMP-KSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFL 627
            +   F +FD DGS ++D  E K  +   G      EI ++   ID++ SGT++F++FL  +   M  K   E I KAF   D    G I+ ++LK V   +       GE   D+  +E +   EA  + DG + ++EFL
Sbjct:   33 IREAFDLFDTDGSGTIDVKELKVAMRALGFEPKKEEIKKMIADIDKEGSGTINFNDFLSMMTQKMSEKDSKEEILKAFRLFDDDGTGKISFKNLKRVAKEL-------GENLTDEELQEMID--EADRDGDGEINEQEFL 163          
BLAST of Calcyphosin protein vs. Ensembl Cavefish
Match: cetn4 (caltractin [Source:NCBI gene;Acc:103037928])

HSP 1 Score: 56.225 bits (134), Expect = 5.269e-9
Identity = 44/140 (31.43%), Postives = 69/140 (49.29%), Query Frame = 1
Query:  211 LSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMP-KSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFL 627
            +   F +FD DGS ++D  E K  +   G      EI ++   ID++ SGT++F++FL  +   M  K   E I KAF   D    G I+ ++LK V   +       GE   D+  +E +   EA  + DG + ++EFL
Sbjct:   32 IREAFDLFDTDGSGTIDVKELKVAMRALGFEPKKEEIKKMIADIDKEGSGTINFNDFLSMMTQKMSEKDSKEEILKAFRLFDDDGTGKISFKNLKRVAKEL-------GENLTDEELQEMID--EADRDGDGEINEQEFL 162          
BLAST of Calcyphosin protein vs. Ensembl Sea Lamprey
Match: si:rp71-17i16.4 (si:rp71-17i16.4 [Source:ZFIN;Acc:ZDB-GENE-090312-215])

HSP 1 Score: 57.7658 bits (138), Expect = 2.842e-10
Identity = 44/149 (29.53%), Postives = 70/149 (46.98%), Query Frame = 1
Query:  220 TFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRV----EMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISAS 654
             F +FD DG   +  +E  K +   G      E+  I  ++D D SGT+DF+EFL+ +   M +       E +++AF  +D   DG I  ++LK +          +GE   D    E +K+    +  DG +  +EFL    GI+AS
Sbjct:   28 AFDMFDADGGGDISTSELGKVMKLLGQTPTKEELDAIIEEVDEDGSGTIDFEEFLVMMVRQMKEESAGQTEEELAEAFRILDTNGDGYIDRDELKDIL-------LNTGENVTDLEMDELMKD--GDKNCDGRLDFDEFLKMMEGIAAS 167          
BLAST of Calcyphosin protein vs. Ensembl Sea Lamprey
Match: CETN1 (centrin 1 [Source:HGNC Symbol;Acc:HGNC:1866])

HSP 1 Score: 56.225 bits (134), Expect = 1.277e-9
Identity = 44/140 (31.43%), Postives = 68/140 (48.57%), Query Frame = 1
Query:  211 LSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMP-KSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFL 627
            +   F +FD DGS ++D  E K  +   G      EI ++    D+D SG ++F++FL  +   M  K   E I KAF   D  + G I+ ++LK V   +       GE   D+  +E +   EA  + DG + +EEFL
Sbjct:   44 IKEAFDLFDADGSGTIDVKELKVAMRALGFEPKKEEIKKMIADFDKDGSGKINFNDFLAMMTQKMSEKDSREEILKAFRLFDDDETGKISFKNLKRVAKEL-------GETLTDEELQEMID--EADRDGDGEINEEEFL 174          
BLAST of Calcyphosin protein vs. Ensembl Sea Lamprey
Match: cetn3 (centrin 3 [Source:ZFIN;Acc:ZDB-GENE-050522-152])

HSP 1 Score: 51.9878 bits (123), Expect = 2.592e-8
Identity = 37/147 (25.17%), Postives = 70/147 (47.62%), Query Frame = 1
Query:  211 LSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFL-IALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGIS 648
            +   F++FD D   ++D++E K  I   G  +   ++ +I    DR+ +G + +D+F  +     + +  ++ + KAF   D+ + G I V DL+ V   +       GE   D+  R  +  F+   + DG + +EEF+    G S
Sbjct:   31 IQEAFELFDTDKDGAIDYHELKVAIRALGFEVSKADVIKIMKDYDREDTGKISYDDFCEVMTERVLERDPLDEVLKAFRLFDEGESGRIGVRDLRRVAREL-------GETVSDEELRAMIDEFDT--DGDGEINQEEFIAIMTGDS 168          
BLAST of Calcyphosin protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006008.1 (pep scaffold:Pmarinus_7.0:GL476713:142259:146509:-1 gene:ENSPMAG00000005430.1 transcript:ENSPMAT00000006008.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.2174 bits (121), Expect = 3.892e-8
Identity = 42/148 (28.38%), Postives = 67/148 (45.27%), Query Frame = 1
Query:  202 IKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKS-RVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCG 642
            I      F +FD DG  ++   E    +   G      E+ ++ N++D D +GT+DF EFL  +   M  +   E I +AF   DK  +G I+  +L+ V + +       GE   D+   E ++  EA  + DG V  EEF+     
Sbjct:   10 IAEFKEAFSLFDKDGDGTITVKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNL-------GEKLTDEEVDEMIR--EADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calcyphosin protein vs. Ensembl Sea Lamprey
Match: CALM3 (calmodulin 3 [Source:HGNC Symbol;Acc:HGNC:1449])

HSP 1 Score: 50.8322 bits (120), Expect = 5.140e-8
Identity = 42/148 (28.38%), Postives = 67/148 (45.27%), Query Frame = 1
Query:  202 IKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKS-RVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCG 642
            I      F +FD DG  ++   E    +   G      E+ ++ N++D D +GT+DF EFL  +   M  +   E I +AF   DK  +G I+  +L+ V + +       GE   D+   E ++  EA  + DG V  EEF+     
Sbjct:   10 IAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNL-------GEKLTDEEVDEMIR--EADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calcyphosin protein vs. Ensembl Yeast
Match: CMD1 (Calmodulin; Ca2+ binding protein that regulates Ca2+ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca2+ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin; binds to the Hog1p MAPK in response to hyperosmotic stress; potentiates membrane tubulation and constriction mediated by the Rvs161p-Rvs167p complex; human CALM1 or CALM2 functionally complement repression induced inviability [Source:SGD;Acc:S000000313])

HSP 1 Score: 46.9802 bits (110), Expect = 7.472e-7
Identity = 27/99 (27.27%), Postives = 51/99 (51.52%), Query Frame = 1
Query:  217 RTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEM-ISKAFAKMDKTKDGAITVEDLKGVYSAV 510
              F +FD D + S+  +E    +   G+     E++++ N+ID D +  ++F EFL  +   +  +  E  + +AF   DK  DG I+  +LK V +++
Sbjct:   15 EAFALFDKDNNGSISSSELATVMRSLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFLALMSRQLKSNDSEQELLEAFKVFDKNGDGLISAAELKHVLTSI 113          
BLAST of Calcyphosin protein vs. Ensembl Nematostella
Match: EDO32961 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7STK4])

HSP 1 Score: 283.108 bits (723), Expect = 3.347e-94
Identity = 132/208 (63.46%), Postives = 163/208 (78.37%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            MA T+R  KEL  +  K      DPVE LRL CLSRG++GIKGL RTFKI DDDG+RSLDFNEFKKG+ DYG++++  E+   F   D D SGT+DFDEFLI LRPPM K+R  +I +AF K+DKT D  ITVEDLKGVY+  HHPK+++GEWTE+Q  R+FL +F++P++ DGI+T +EFLNYY G+SASID+DAYFDLMMRNAYKL
Sbjct:    1 MAATARHEKELIEKCKKNAGQTKDPVESLRLACLSRGSSGIKGLGRTFKIMDDDGNRSLDFNEFKKGLRDYGVMLEPKEVKRTFEAFDTDGSGTIDFDEFLIRLRPPMSKARKNVIQQAFNKLDKTGDNNITVEDLKGVYNVKHHPKYQNGEWTEEQCLRKFLDSFDSPDDKDGIITYDEFLNYYSGVSASIDSDAYFDLMMRNAYKL 208          
BLAST of Calcyphosin protein vs. Ensembl Nematostella
Match: EDO32962 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7STK5])

HSP 1 Score: 173.326 bits (438), Expect = 8.543e-52
Identity = 84/174 (48.28%), Postives = 122/174 (70.11%), Query Frame = 1
Query:   91 TSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEA-PEEIDGIV 609
            T  + +EL++++ K  +NA  P+E+L      RG  GIK   R F+IFDDDG+R+L+ +EF+KG+ D+G  + ++E+ ++F Q D+D SG+LDF+EFL A+RP M K+RVE+I KAF KMD T DG IT+ DLK VY+A    +++SGE TE +VF++FL  FE  P + DG V
Sbjct:    5 TMADERELKIKAKKMEENAKTPIEKLHAAMAGRGTCGIKMFGRAFRIFDDDGNRALNIDEFRKGMQDFGTKLTDDEVKQLFAQFDKDGSGSLDFEEFLRAVRPSMSKNRVEIIMKAFHKMDTTGDGVITIADLKKVYNARQDKRYQSGEMTEAEVFQKFLATFEPDPAKRDGKV 178          
BLAST of Calcyphosin protein vs. Ensembl Nematostella
Match: EDO33723 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRH0])

HSP 1 Score: 136.732 bits (343), Expect = 1.702e-38
Identity = 76/162 (46.91%), Postives = 104/162 (64.20%), Query Frame = 1
Query:  223 FKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEA-PEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            F+IFDDDG+++L+ +EF+KG+ DYG  + ++     F+    D           + L     + R  +I KAF KMD T DG IT+ DLK VY+A    +++SGE TE +VF++FL  FE  P + DG VTK EFL+YY G+S SID+DAYFDLMMR A+KL
Sbjct:    3 FRIFDDDGNKALNIDEFRKGMQDYGTKLTDDVSFTHFSAQSTD-----------LLLIFQGLELRFRIIMKAFHKMDTTGDGVITIADLKKVYNARQDKRYQSGEMTEAEVFQKFLATFEPDPAKRDGKVTKGEFLDYYNGVSVSIDDDAYFDLMMRQAWKL 153          
BLAST of Calcyphosin protein vs. Ensembl Nematostella
Match: EDO37727 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SF01])

HSP 1 Score: 89.7373 bits (221), Expect = 6.136e-20
Identity = 57/178 (32.02%), Postives = 89/178 (50.00%), Query Frame = 1
Query:  166 LRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAY 699
            +R     R +  + G+ R F+  D DG R   F EF +       V++   +  ++N +D +  G LD+ EF+      M ++R   + K F K D +K G + + ++K  +SA  HPK  SG   E +  + FL +FE  E   G VT  EF +YY G+S  I +D  F  MM+N +
Sbjct:  149 VRRQLAQRASKTLMGMGRHFRTLDKDGHRL--FEEFWE-------VVEKEVLGGLWNLLDVNGDGALDYGEFVRGFIGEMNETRKAYVRKVFHKFDPSKTGLVELNNMKKFFSAKKHPKVLSGRLKEHEAQQMFLSSFENCEH-RGEVTYAEFEDYYEGVSIGIASDEEFVAMMKNCW 316          
BLAST of Calcyphosin protein vs. Ensembl Nematostella
Match: EDO40775 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S690])

HSP 1 Score: 60.8474 bits (146), Expect = 4.193e-11
Identity = 47/140 (33.57%), Postives = 70/140 (50.00%), Query Frame = 1
Query:  211 LSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMP-KSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFL 627
            +   F +FD DGS ++D  E K  +   G      EI ++ + ID+D SGT+DF+EFL  +   M  K   E I KAF   D  + G I+ ++LK V   +       GE   D+  +E +   EA  + DG + + EFL
Sbjct:   23 IREAFDLFDTDGSGTIDAKELKVAMRALGFEPKKEEIKKMISDIDKDGSGTIDFNEFLQMMTAKMSEKDSKEEILKAFKLFDDDETGKISFKNLKRVAKEL-------GENLTDEELQEMID--EADRDGDGEINEAEFL 153          
BLAST of Calcyphosin protein vs. Ensembl Medaka
Match: capslb (calcyphosine like [Source:NCBI gene;Acc:101171382])

HSP 1 Score: 272.707 bits (696), Expect = 6.793e-89
Identity = 124/209 (59.33%), Postives = 165/209 (78.95%), Query Frame = 1
Query:   79 TMAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
             MA TSR + E+ V + ++L   +DPVE+LRL CL RG++GIKGL RTF++ DD+ +RSLD  EF KG+ DYG++M+  E   +F   DRD SGT+ FDEFLIALRPPM ++R+E++ +AF KMDKT DG ITVEDL+GVY+A +HPK+++GE +EDQVFR FL +F++P + DG VTKEEF++YY G+SASID+D YF LMMRNA+KL
Sbjct:   50 AMAGTSRHDHEMAVMAKRQLSQCSDPVEKLRLQCLIRGSSGIKGLGRTFRVMDDNQNRSLDLKEFLKGLNDYGLLMEKEEAAALFQHFDRDGSGTISFDEFLIALRPPMSRARLEVVMQAFRKMDKTGDGVITVEDLRGVYNAKYHPKYQNGELSEDQVFRTFLDSFDSPYDKDGKVTKEEFIDYYSGVSASIDSDVYFILMMRNAWKL 258          
BLAST of Calcyphosin protein vs. Ensembl Medaka
Match: cetn2 (caltractin [Source:NCBI gene;Acc:101170769])

HSP 1 Score: 54.299 bits (129), Expect = 2.552e-8
Identity = 43/141 (30.50%), Postives = 67/141 (47.52%), Query Frame = 1
Query:  211 LSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMP-KSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLN 630
            +   F++FD DGS  +D  + K  +   G      EI ++  ++D+D +G + F +FL  +   M  K   E I KAF   D  + G I+  +LK V   +       GE   D+  +E +   EA  + DG V +EEFL 
Sbjct:   36 IREAFELFDTDGSGFIDVKDLKVAMRALGFEPKKEEIKKMIGEVDKDATGKISFADFLTVMTQKMAEKDSKEEILKAFRLFDDDETGKISFRNLKRVAKEL-------GENLTDEELQEMID--EADRDGDGEVNQEEFLR 167          
BLAST of Calcyphosin protein vs. Ensembl Medaka
Match: cetn2 (caltractin [Source:NCBI gene;Acc:101170769])

HSP 1 Score: 54.299 bits (129), Expect = 2.552e-8
Identity = 43/141 (30.50%), Postives = 67/141 (47.52%), Query Frame = 1
Query:  211 LSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMP-KSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLN 630
            +   F++FD DGS  +D  + K  +   G      EI ++  ++D+D +G + F +FL  +   M  K   E I KAF   D  + G I+  +LK V   +       GE   D+  +E +   EA  + DG V +EEFL 
Sbjct:   36 IREAFELFDTDGSGFIDVKDLKVAMRALGFEPKKEEIKKMIGEVDKDATGKISFADFLTVMTQKMAEKDSKEEILKAFRLFDDDETGKISFRNLKRVAKEL-------GENLTDEELQEMID--EADRDGDGEVNQEEFLR 167          
BLAST of Calcyphosin protein vs. Ensembl Medaka
Match: ENSORLT00000004736.2 (calcium-binding protein 2-like [Source:NCBI gene;Acc:101162482])

HSP 1 Score: 52.373 bits (124), Expect = 1.044e-7
Identity = 43/159 (27.04%), Postives = 68/159 (42.77%), Query Frame = 1
Query:  175 MCLSR---GATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEM-----ISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFL 627
            +CL         I  L   FK FD D    L++ +  + +   G +    E+ EI  QI     G +DFD+F+  + P M     +M     +  AF++ D+  DG I  +++K     +   K K GE  E  + +E   N       DG +  EEF+
Sbjct:    4 LCLQERELAQAEIDELQEAFKEFDYDQDGYLNYKDVAECMRTMGYMPTEMELLEIVQQIKMRMGGLMDFDDFIELMAPRMMGETTDMLGLKEVQSAFSQFDQDGDGKINQDEMKEALKTLLGEKLKKGEQEE--ILKELDVN------ADGNIDFEEFV 154          
BLAST of Calcyphosin protein vs. Ensembl Medaka
Match: ENSORLT00000022188.2 (calmodulin [Source:NCBI gene;Acc:100049422])

HSP 1 Score: 51.2174 bits (121), Expect = 2.476e-7
Identity = 42/144 (29.17%), Postives = 67/144 (46.53%), Query Frame = 1
Query:  202 IKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKS-RVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLN 630
            I      F +FD DG  ++   E    +   G      E+ ++ N++D D +GT+DF EFL  +   M  +   E I +AF   DK  +G I+  +L+ V + +       GE   D+   E ++  EA  + DG V  EEF+ 
Sbjct:   24 IAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNL-------GEKLTDEEVDEMIR--EADIDGDGQVNYEEFVQ 158          
BLAST of Calcyphosin protein vs. Planmine SMEST
Match: SMESG000005291.1 (SMESG000005291.1)

HSP 1 Score: 434.876 bits (1117), Expect = 4.375e-153
Identity = 209/211 (99.05%), Postives = 210/211 (99.53%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKLYSF 714
            MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFK+FDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPM KSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKLYSF
Sbjct:    1 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKVFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMSKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKLYSF 211          
BLAST of Calcyphosin protein vs. Planmine SMEST
Match: SMESG000005291.1 (SMESG000005291.1)

HSP 1 Score: 428.328 bits (1100), Expect = 6.166e-151
Identity = 206/208 (99.04%), Postives = 207/208 (99.52%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFK+FDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPM KSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL
Sbjct:    1 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKVFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMSKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 208          
BLAST of Calcyphosin protein vs. Planmine SMEST
Match: SMESG000025859.1 (SMESG000025859.1)

HSP 1 Score: 308.916 bits (790), Expect = 5.661e-104
Identity = 146/208 (70.19%), Postives = 176/208 (84.62%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            MA T+R N+ELQ++S +KL++A+DP+E+LRL CL+RGATGIKGL R FKI+DDDG+ +LDF EF KG+ DYG  +   E+  IF  +D+DKSGTLDFDEFLIALRPPM KSRV ++ +AF K+DKTKDG ITVEDLKGVY+   HPK+ SGE TE+++F EFLKNFEAP+EIDG VTKEEFLNYY G+SASIDNDAYFDLMMRNAYKL
Sbjct:    1 MALTNRGNRELQMKSKEKLKSASDPMEKLRLQCLARGATGIKGLGRAFKIYDDDGNNTLDFREFNKGVNDYGCDLSKEEVQFIFEGLDKDKSGTLDFDEFLIALRPPMTKSRVALVIQAFNKLDKTKDGFITVEDLKGVYNVSKHPKYISGERTEEELFLEFLKNFEAPDEIDGKVTKEEFLNYYSGVSASIDNDAYFDLMMRNAYKL 208          
BLAST of Calcyphosin protein vs. Planmine SMEST
Match: SMESG000017753.1 (SMESG000017753.1)

HSP 1 Score: 245.743 bits (626), Expect = 2.562e-79
Identity = 114/208 (54.81%), Postives = 158/208 (75.96%), Query Frame = 1
Query:   82 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKIFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 705
            MAF+ +++ EL++++ K+L  A DP+ +LRL CL  GA+GIKGLSR F+I +DD  R L   EFK GI  +G+++ ++EI ++F  +DRD S T+   EFL ALRPP+  +R+++I KAF  MDKT DG IT+EDLK VY+A  HPK ++GEW+E +VF+EFLK+FEAP + DGIVT +EFL+YY  IS SIDN+ YFDLMMRN++K+
Sbjct:    1 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 208          
BLAST of Calcyphosin protein vs. Planmine SMEST
Match: SMESG000072346.1 (SMESG000072346.1)

HSP 1 Score: 97.8265 bits (242), Expect = 1.310e-24
Identity = 44/75 (58.67%), Postives = 60/75 (80.00%), Query Frame = 1
Query:  478 VEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYK 702
            ++D+KGVY    + ++ +G+WTE++ F EFLKNFE   +IDGIVT EEFL+YY  ISASID D+YF+LMMRN++K
Sbjct:   15 IDDVKGVYDIRKNSRYLNGDWTEEKCFLEFLKNFENQNQIDGIVTYEEFLDYYSVISASIDKDSYFNLMMRNSFK 89          
The following BLAST results are available for this feature:
BLAST of Calcyphosin protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CAPSL1.492e-8759.13calcyphosine like [Source:HGNC Symbol;Acc:HGNC:283... [more]
CAPSL1.492e-8759.13calcyphosine like [Source:HGNC Symbol;Acc:HGNC:283... [more]
CAPSL1.492e-8759.13calcyphosine like [Source:HGNC Symbol;Acc:HGNC:283... [more]
CAPSL3.768e-7858.42calcyphosine like [Source:HGNC Symbol;Acc:HGNC:283... [more]
CAPSL4.407e-7057.30calcyphosine like [Source:HGNC Symbol;Acc:HGNC:283... [more]
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BLAST of Calcyphosin protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cal-17.334e-929.58Calmodulin-like protein [Source:UniProtKB/Swiss-P... [more]
cal-18.153e-930.15Calmodulin-like protein [Source:UniProtKB/Swiss-P... [more]
E02A10.31.281e-825.36pep chromosome:WBcel235:V:12585099:12587991:1 gene... [more]
E02A10.31.668e-825.36pep chromosome:WBcel235:V:12584830:12587945:1 gene... [more]
E02A10.32.137e-825.36pep chromosome:WBcel235:V:12586477:12587945:1 gene... [more]
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BLAST of Calcyphosin protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG313452.913e-7759.61gene:FBgn0051345 transcript:FBtr0082689[more]
CG101262.623e-6955.43gene:FBgn0038088 transcript:FBtr0082686[more]
CG101267.510e-6955.43gene:FBgn0038088 transcript:FBtr0082687[more]
CG318024.997e-829.58gene:FBgn0051802 transcript:FBtr0081058[more]
CG174931.199e-732.14gene:FBgn0040010 transcript:FBtr0332630[more]
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BLAST of Calcyphosin protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
capslb1.102e-9160.48calcyphosine-like b [Source:ZFIN;Acc:ZDB-GENE-0504... [more]
capsla4.899e-7756.68calcyphosine-like a [Source:ZFIN;Acc:ZDB-GENE-0301... [more]
kcnip29.869e-829.52Kv channel interacting protein 2 [Source:ZFIN;Acc:... [more]
cetn41.083e-730.71centrin 4 [Source:ZFIN;Acc:ZDB-GENE-030826-21][more]
si:ch211-145b13.52.132e-731.48si:ch211-145b13.5 [Source:ZFIN;Acc:ZDB-GENE-090313... [more]
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BLAST of Calcyphosin protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
capsl1.063e-9561.06calcyphosine like [Source:NCBI gene;Acc:549278][more]
capsl1.590e-9359.62calcyphosine like [Source:NCBI gene;Acc:549278][more]
capsl6.603e-9161.11calcyphosine like [Source:NCBI gene;Acc:549278][more]
caps8.061e-7852.40calcyphosine [Source:NCBI gene;Acc:100037881][more]
ENSXETT00000016513.17.353e-1932.37calcyphosine 2 [Source:NCBI gene;Acc:100380015][more]
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BLAST of Calcyphosin protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Capsl1.798e-9360.58calcyphosine-like [Source:MGI Symbol;Acc:MGI:19228... [more]
Capsl1.119e-3858.10calcyphosine-like [Source:MGI Symbol;Acc:MGI:19228... [more]
Caps26.861e-1225.41calcyphosphine 2 [Source:MGI Symbol;Acc:MGI:244198... [more]
Caps26.998e-1225.41calcyphosphine 2 [Source:MGI Symbol;Acc:MGI:244198... [more]
Cetn15.033e-930.88centrin 1 [Source:MGI Symbol;Acc:MGI:1347086][more]
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BLAST of Calcyphosin protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6P8Y1|CAPSL_MOUSE1.258e-9260.58Calcyphosin-like protein OS=Mus musculus OX=10090 ... [more]
sp|Q8WWF8|CAPSL_HUMAN7.166e-8759.13Calcyphosin-like protein OS=Homo sapiens OX=9606 G... [more]
sp|Q0VCC0|CAYP1_BOVIN1.704e-6753.55Calcyphosin OS=Bos taurus OX=9913 GN=CAPS PE=2 SV=... [more]
sp|Q13938|CAYP1_HUMAN1.746e-6554.10Calcyphosin OS=Homo sapiens OX=9606 GN=CAPS PE=1 S... [more]
sp|P41150|CAYP1_RABIT4.657e-6552.46Calcyphosin OS=Oryctolagus cuniculus OX=9986 GN=CA... [more]
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BLAST of Calcyphosin protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267H5522.421e-9364.42Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A564YA369.702e-9363.46Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
A0A267FSW19.807e-9363.94Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267H6P01.348e-9262.86Uncharacterized protein OS=Macrostomum lignano OX=... [more]
F7EF001.668e-9261.06Uncharacterized protein OS=Xenopus tropicalis OX=8... [more]
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BLAST of Calcyphosin protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
capslb8.104e-8959.13calcyphosine like [Source:NCBI gene;Acc:103021302][more]
capslb1.074e-8859.62calcyphosine like [Source:NCBI gene;Acc:103021302][more]
capsla9.586e-8160.75calcyphosin-like protein [Source:NCBI gene;Acc:103... [more]
cetn44.875e-931.43caltractin [Source:NCBI gene;Acc:103037928][more]
cetn45.269e-931.43caltractin [Source:NCBI gene;Acc:103037928][more]
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BLAST of Calcyphosin protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
si:rp71-17i16.42.842e-1029.53si:rp71-17i16.4 [Source:ZFIN;Acc:ZDB-GENE-090312-2... [more]
CETN11.277e-931.43centrin 1 [Source:HGNC Symbol;Acc:HGNC:1866][more]
cetn32.592e-825.17centrin 3 [Source:ZFIN;Acc:ZDB-GENE-050522-152][more]
ENSPMAT00000006008.13.892e-828.38pep scaffold:Pmarinus_7.0:GL476713:142259:146509:-... [more]
CALM35.140e-828.38calmodulin 3 [Source:HGNC Symbol;Acc:HGNC:1449][more]
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BLAST of Calcyphosin protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
CMD17.472e-727.27Calmodulin; Ca2+ binding protein that regulates Ca... [more]
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BLAST of Calcyphosin protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO329613.347e-9463.46Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO329628.543e-5248.28Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO337231.702e-3846.91Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO377276.136e-2032.02Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO407754.193e-1133.57Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Calcyphosin protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
capslb6.793e-8959.33calcyphosine like [Source:NCBI gene;Acc:101171382][more]
cetn22.552e-830.50caltractin [Source:NCBI gene;Acc:101170769][more]
cetn22.552e-830.50caltractin [Source:NCBI gene;Acc:101170769][more]
ENSORLT00000004736.21.044e-727.04calcium-binding protein 2-like [Source:NCBI gene;A... [more]
ENSORLT00000022188.22.476e-729.17calmodulin [Source:NCBI gene;Acc:100049422][more]
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BLAST of Calcyphosin protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000005291.14.375e-15399.05SMESG000005291.1[more]
SMESG000005291.16.166e-15199.04SMESG000005291.1[more]
SMESG000025859.15.661e-10470.19SMESG000025859.1[more]
SMESG000017753.12.562e-7954.81SMESG000017753.1[more]
SMESG000072346.11.310e-2458.67SMESG000072346.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015209 ID=SMED30015209|Name=Calcyphosin protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1412bp
ACGGTAGCAATATTAAACATTATCAATAATTAAATTTTACTTAAATTATT
TCTTATAGACTTATTTTACAATTATTTTACAATGGCTTTCACATCCAGAG
AAAATAAAGAATTGCAAGTTCAATCTAATAAAAAACTTCAAAATGCGACT
GATCCAGTTGAAAGATTAAGATTAATGTGTTTAAGTCGTGGAGCAACTGG
AATAAAGGGATTATCTCGGACATTTAAGATATTCGATGACGATGGAAGTA
GGTCTTTGGATTTTAATGAATTTAAAAAAGGAATTGCCGATTATGGTATT
GTTATGGATAACAATGAAATACACGAAATATTTAACCAAATTGATAGAGA
TAAAAGCGGAACTCTTGATTTTGATGAATTTCTCATAGCATTAAGACCTC
CTATGCCAAAATCAAGAGTAGAAATGATTAGTAAGGCTTTTGCTAAAATG
GATAAAACAAAAGATGGAGCAATAACGGTTGAAGATTTGAAAGGTGTCTA
TAGTGCTGTTCACCATCCAAAATTTAAGAGTGGAGAGTGGACCGAAGATC
AAGTATTTCGTGAATTCCTGAAAAACTTTGAAGCGCCCGAAGAAATCGAC
GGCATTGTGACCAAGGAAGAATTCCTTAATTATTACTGTGGAATCAGTGC
ATCAATTGACAATGATGCTTATTTTGATTTAATGATGCGAAATGCGTATA
AATTGTACAGTTTTGTTGATTTTTTTGGCTTTTTCACCAGCACGTCGCTT
GCTAAGTAAATATAATCAGCACAGTGCTATATATGAATTTCAATTACTAA
TTGGCACGATTTCACAATTATTTTTTATTTGATTTATTAATATGTCAAAC
TAAAAATGTGCCTAGTCAATATTTTTTTTGGAATTCACTTTTACTAATAA
TCTGCCAGGCCATATTTATGTATGAATGATATGGAAATATCTTCTCACGT
AATTCACAATTTCAATGGTAAGCCATCAGCAAAACAAGTAGCATGGAAAA
ATTTCGTAAACATTCCTGAAAATACTAATTTTTAAATTTTTTATATTGAA
AAAATTGCTTTGTTTTAATTGATGAAAAATTAATAATATTAACTTAATCC
AAACTAGGCATTTATTTTCAATATTTTGACTATAAATGAAGCTGCGAGGG
AAATGGTAAAATAAACCGTAAAATATTTTCTGAAATTGTATTCCAGAGAA
TCTATTTCTTTTTTCGACTAATATTGTACTCTTTACCAGTAAACCGTGAG
ATAAATGATTTCGAAAATATTTTTAAAATATCAACGAATTCCTGTCCATT
ATGAATATTAATTCCGTATTTTATTAAACAAGCTTCTAAAATATGCTGAG
ATCTTACATTAAAATTTTGTTTTAAATATTATTCTTAGGGTTCTTATAGC
GCTTTCGTTGTT
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protein sequence of SMED30015209-orf-1

>SMED30015209-orf-1 ID=SMED30015209-orf-1|Name=SMED30015209-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=226bp
MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKI
FDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEF
LIALRPPMPKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKS
GEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDL
MMRNAYKLYSFVDFFGFFTSTSLAK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR029633CAPSL
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
GO:0046872metal ion binding
Vocabulary: cellular component
TermDefinition
GO:0005575cellular_component
GO:0005737cytoplasm
Vocabulary: biological process
TermDefinition
GO:0008150biological_process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 79..107
e-value: 6.4E-5
score: 32.3
coord: 115..143
e-value: 1.0
score: 17.7
coord: 43..71
e-value: 0.64
score: 19.1
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 78..140
e-value: 7.5E-11
score: 42.3
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 39..74
score: 10.664
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 173..191
score: 5.113
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 111..146
score: 11.529
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 75..110
score: 13.007
IPR002048EF-hand domainCDDcd00051EFhcoord: 44..101
e-value: 3.4777E-13
score: 60.2541
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 109..209
e-value: 3.9E-33
score: 116.0
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 23..108
e-value: 2.2E-21
score: 77.4
NoneNo IPR availablePANTHERPTHR34524FAMILY NOT NAMEDcoord: 1..208
IPR029633Calcyphosin-like proteinPANTHERPTHR34524:SF5CALCYPHOSIN-LIKE PROTEINcoord: 1..208
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 88..100
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 52..64
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 41..207