SMED30015166
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30015166 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of SMED30015166 vs. Planmine SMEST
Match: SMESG000074160.1 (SMESG000074160.1) HSP 1 Score: 251.136 bits (640), Expect = 3.572e-96 Identity = 156/236 (66.10%), Postives = 197/236 (83.47%), Query Frame = -3 Query: 299 AMMNQSEIKNQIIKEVENKESGAVIENQPEQLKETKEEAEQWRQKTIRLKEMLLAVEEELMKETIKTKIMREKWERQIEKKIVYPEGKEKTY*ERDEELMREFIQNQTRIANHRKEIRTLEQDKQDQQNEIEKLRXXXXXXXXXXXXXXXXXXXILNQMQEEIEQMGTKYRIERTKRTSMEQTKEAAKRQASYWKTKWQDLKERTRNDE*SRPRYYTQMISEGEERTRAYKAK*EN 1006 +MN +E K +++KE E ES I++QPE +K T EE E WRQ+T++LKEMLL EEELM+ET+KTK+M+EKWE+Q+E+KI+YPEGKEKTY ER+EE+M+EF+QNQTRI N RKEI+TLEQ+KQDQQ+EIEKLRE+IK KE EI+EEGEETIKI+ QMQEEI Q+ T+YRIE+TKRT MEQ KE AKRQA YWK KW + +E++R++ SR R+Y Q I+E E+RTRAYKAK EN Sbjct: 4949 TIMNPNETK-EVVKEAE--ESKPAIKDQPESIKVTSEEVELWRQRTLQLKEMLLIAEEELMQETLKTKMMKEKWEKQLEQKIIYPEGKEKTYQEREEEMMKEFMQNQTRIKNQRKEIQTLEQEKQDQQSEIEKLREVIKDKEAEIREEGEETIKIIQQMQEEIGQISTQYRIEKTKRTHMEQAKEWAKRQARYWKIKWTEDREQSRSNWESRIRHYIQQIAEEEDRTRAYKAKWEN 5181 HSP 2 Score: 120.168 bits (300), Expect = 3.572e-96 Identity = 66/102 (64.71%), Postives = 83/102 (81.37%), Query Frame = -1 Query: 1 ERMMTQITDQARRWRIQYKILEDANAKQFEYIVKLVEHETVWQQTKIKEILLEQERTLKQRQEQQRKTEIKEESIKMEARKWKEQYQEAKKEIGLLPERILN 306 ERM+TQITDQAR+WRI+ +I EDANAKQ YIVKL E E ++QT+IKE+ +EQ+ +QR+ Q+ KTE KE+ IK EAR+WK QYQEAKKEI LLP++ILN Sbjct: 5209 ERMLTQITDQARKWRIECRIFEDANAKQSNYIVKL-EQEIAYKQTRIKEMQIEQDEAHRQRRIQRIKTEKKEKWIKSEARRWKNQYQEAKKEIRLLPKKILN 5309
BLAST of SMED30015166 vs. Planmine SMEST
Match: SMESG000023758.1 (SMESG000023758.1) HSP 1 Score: 239.58 bits (610), Expect = 2.195e-92 Identity = 150/234 (64.10%), Postives = 193/234 (82.48%), Query Frame = -3 Query: 299 MNQSEIKNQIIKEVENKESGAVIENQPEQLKETKEEAEQWRQKTIRLKEMLLAVEEELMKETIKTKIMREKWERQIEKKIVYPEGKEKTY*ERDEELMREFIQNQTRIANHRKEIRTLEQDKQDQQNEIEKLRXXXXXXXXXXXXXXXXXXXILNQMQEEIEQMGTKYRIERTKRTSMEQTKEAAKRQASYWKTKWQDLKERTRNDE*SRPRYYTQMISEGEERTRAYKAK*EN 1000 MNQ+E K +++ E E ES I++QPE +K EE E WRQ+T++LKEMLL EEEL++ET+KTK+M+EKWE+Q+E+KI+YPEG+EKTY ER+EE+M+EF+QNQTRI + RKEIR LEQ+KQDQQ+EIE+LRE+IK KE EI+EEGEETIKI+ QMQEEI Q+ T+YRIE+TKRT +EQ KE AKRQA YWK KW + +E +R++ SR R+Y Q I+E E+RTRAYKAK EN Sbjct: 4428 MNQNETK-EVVTEAE--ESKPTIKDQPESIKVINEEVELWRQRTLQLKEMLLIAEEELVQETLKTKMMKEKWEKQLEQKIIYPEGEEKTYQEREEEMMKEFMQNQTRIISQRKEIRMLEQEKQDQQSEIERLREVIKDKEAEIREEGEETIKIIQQMQEEIGQISTQYRIEKTKRTHLEQAKEWAKRQARYWKIKWTEDREHSRSNWESRIRHYIQQIAEEEDRTRAYKAKWEN 4658 HSP 2 Score: 119.013 bits (297), Expect = 2.195e-92 Identity = 67/102 (65.69%), Postives = 84/102 (82.35%), Query Frame = -1 Query: 1 ERMMTQITDQARRWRIQYKILEDANAKQFEYIVKLVEHETVWQQTKIKEILLEQERTLKQRQEQQRKTEIKEESIKMEARKWKEQYQEAKKEIGLLPERILN 306 ERM+TQITDQAR+WRI+ KI EDANAKQ YIVKL E E +QQT++KE+ +EQ+ ++ Q+QQ KTEIKE++IK EA+KWKE +QEAKKEI LL +RILN Sbjct: 4686 ERMLTQITDQARKWRIECKIWEDANAKQSSYIVKL-EQEVEYQQTRVKEMQIEQDEAHRKEQKQQWKTEIKEKAIKREAKKWKEHFQEAKKEIRLLLKRILN 4786
BLAST of SMED30015166 vs. Planmine SMEST
Match: SMESG000053822.1 (SMESG000053822.1) HSP 1 Score: 237.654 bits (605), Expect = 9.869e-92 Identity = 150/235 (63.83%), Postives = 193/235 (82.13%), Query Frame = -3 Query: 299 MMNQSEIKNQIIKEVENKESGAVIENQPEQLKETKEEAEQWRQKTIRLKEMLLAVEEELMKETIKTKIMREKWERQIEKKIVYPEGKEKTY*ERDEELMREFIQNQTRIANHRKEIRTLEQDKQDQQNEIEKLRXXXXXXXXXXXXXXXXXXXILNQMQEEIEQMGTKYRIERTKRTSMEQTKEAAKRQASYWKTKWQDLKERTRNDE*SRPRYYTQMISEGEERTRAYKAK*EN 1003 +MN +E K +++KE E ES I++Q E +K T EE E WRQ+T++LKEMLL EEEL++ET+KTK+M+EKWE+Q+E+KI YPEGKEKTY ER+EE+M+EF+QNQTRI N RKEIR LEQ+KQDQQ+EIE+LRE+IK KE EI+EEGEETIKI+ QMQEEI Q+ T+YRIE+TKRT +EQ KE AKRQA +WK KW + +E++R++ SR R+Y Q I+E E+RTRAYK K EN Sbjct: 4970 IMNPNETK-EVVKEAE--ESKPAIKDQSESIKVTSEEVELWRQRTLQLKEMLLIAEEELVQETLKTKMMKEKWEKQLEQKITYPEGKEKTYQEREEEMMKEFMQNQTRIINQRKEIRMLEQEKQDQQSEIERLREVIKDKEAEIREEGEETIKIIQQMQEEIGQISTQYRIEKTKRTHLEQAKEWAKRQALHWKIKWTEDREQSRSNWESRIRHYIQQIAEEEDRTRAYKTKWEN 5201 HSP 2 Score: 119.013 bits (297), Expect = 9.869e-92 Identity = 64/102 (62.75%), Postives = 85/102 (83.33%), Query Frame = -1 Query: 1 ERMMTQITDQARRWRIQYKILEDANAKQFEYIVKLVEHETVWQQTKIKEILLEQERTLKQRQEQQRKTEIKEESIKMEARKWKEQYQEAKKEIGLLPERILN 306 ERM+TQITDQAR+WRI+ ++ EDANAKQ YIVKL + E +QQT++KE+ +EQ+ ++ Q++Q KTEIKE++IK EA+KWKE +QEAKKEI LLP+RILN Sbjct: 5229 ERMLTQITDQARKWRIECRLWEDANAKQSSYIVKL-KQEVEYQQTRVKEMQIEQDEAHRKEQKRQWKTEIKEKAIKREAKKWKEHFQEAKKEIRLLPKRILN 5329
BLAST of SMED30015166 vs. Planmine SMEST
Match: SMESG000066529.1 (SMESG000066529.1) HSP 1 Score: 238.424 bits (607), Expect = 8.778e-91 Identity = 151/236 (63.98%), Postives = 192/236 (81.36%), Query Frame = -3 Query: 299 AMMNQSEIKNQIIKEVENKESGAVIENQPEQLKETKEEAEQWRQKTIRLKEMLLAVEEELMKETIKTKIMREKWERQIEKKIVYPEGKEKTY*ERDEELMREFIQNQTRIANHRKEIRTLEQDKQDQQNEIEKLRXXXXXXXXXXXXXXXXXXXILNQMQEEIEQMGTKYRIERTKRTSMEQTKEAAKRQASYWKTKWQDLKERTRNDE*SRPRYYTQMISEGEERTRAYKAK*EN 1006 +MN +EIK +++ E E ES I++Q E ++ T EE E WRQ+T++LKEMLL VEEEL++ET+KTK+M+EKWE+Q+E+K+ YPEGKEKTY ER+EE M+EF+QNQTRI N RKEI+TLEQ+KQDQQ+EIE+LRE+IK KE EI+EEGEETIKI+ QMQEEI Q+ T+YRIE+TKRT +EQ KE AKRQA +WK KW KER RND +R R+Y Q I+E E+RTRAYK K +N Sbjct: 4201 TIMNPNEIK-EVVTEAE--ESKPAIKDQSESIEVTSEEVEVWRQRTLQLKEMLLIVEEELVQETLKTKMMKEKWEKQLEQKVTYPEGKEKTYQEREEERMKEFMQNQTRIKNQRKEIQTLEQEKQDQQSEIERLREVIKDKEAEIREEGEETIKIIQQMQEEIGQISTQYRIEKTKRTHLEQAKEWAKRQALHWKAKWYGEKERARNDWEARIRHYIQQIAEEEDRTRAYKTKWKN 4433 HSP 2 Score: 115.161 bits (287), Expect = 8.778e-91 Identity = 64/102 (62.75%), Postives = 83/102 (81.37%), Query Frame = -1 Query: 1 ERMMTQITDQARRWRIQYKILEDANAKQFEYIVKLVEHETVWQQTKIKEILLEQERTLKQRQEQQRKTEIKEESIKMEARKWKEQYQEAKKEIGLLPERILN 306 ERM+ Q T +AR+W+ +Y++ + NA Q+E+I K +HE VWQQT++KE+L+EQE+TLKQRQ QQ KTE KE+ IK EARKWK QYQEAKKEI LLP++ILN Sbjct: 4461 ERMVIQATGEARKWKDKYRLSKIENATQWEHIQKQ-DHEIVWQQTRMKEMLVEQEKTLKQRQIQQIKTEDKEKWIKSEARKWKNQYQEAKKEIRLLPKKILN 4561
BLAST of SMED30015166 vs. Planmine SMEST
Match: SMESG000066529.1 (SMESG000066529.1) HSP 1 Score: 238.424 bits (607), Expect = 9.000e-91 Identity = 151/235 (64.26%), Postives = 192/235 (81.70%), Query Frame = -3 Query: 299 MMNQSEIKNQIIKEVENKESGAVIENQPEQLKETKEEAEQWRQKTIRLKEMLLAVEEELMKETIKTKIMREKWERQIEKKIVYPEGKEKTY*ERDEELMREFIQNQTRIANHRKEIRTLEQDKQDQQNEIEKLRXXXXXXXXXXXXXXXXXXXILNQMQEEIEQMGTKYRIERTKRTSMEQTKEAAKRQASYWKTKWQDLKERTRNDE*SRPRYYTQMISEGEERTRAYKAK*EN 1003 +MN +EIK +++ E E ES I++Q E ++ T EE E WRQ+T++LKEMLL VEEEL++ET+KTK+M+EKWE+Q+E+K+ YPEGKEKTY ER+EE M+EF+QNQTRI N RKEI+TLEQ+KQDQQ+EIE+LRE+IK KE EI+EEGEETIKI+ QMQEEI Q+ T+YRIE+TKRT +EQ KE AKRQA +WK KW KER RND +R R+Y Q I+E E+RTRAYK K +N Sbjct: 4206 IMNPNEIK-EVVTEAE--ESKPAIKDQSESIEVTSEEVEVWRQRTLQLKEMLLIVEEELVQETLKTKMMKEKWEKQLEQKVTYPEGKEKTYQEREEERMKEFMQNQTRIKNQRKEIQTLEQEKQDQQSEIERLREVIKDKEAEIREEGEETIKIIQQMQEEIGQISTQYRIEKTKRTHLEQAKEWAKRQALHWKAKWYGEKERARNDWEARIRHYIQQIAEEEDRTRAYKTKWKN 4437 HSP 2 Score: 114.775 bits (286), Expect = 9.000e-91 Identity = 64/102 (62.75%), Postives = 83/102 (81.37%), Query Frame = -1 Query: 1 ERMMTQITDQARRWRIQYKILEDANAKQFEYIVKLVEHETVWQQTKIKEILLEQERTLKQRQEQQRKTEIKEESIKMEARKWKEQYQEAKKEIGLLPERILN 306 ERM+ Q T +AR+W+ +Y++ + NA Q+E+I K +HE VWQQT++KE+L+EQE+TLKQRQ QQ KTE KE+ IK EARKWK QYQEAKKEI LLP++ILN Sbjct: 4465 ERMVIQATGEARKWKDKYRLSKIENATQWEHIQKQ-DHEIVWQQTRMKEMLVEQEKTLKQRQIQQIKTEDKEKWIKSEARKWKNQYQEAKKEIRLLPKKILN 4565 The following BLAST results are available for this feature:
BLAST of SMED30015166 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30015166 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30015166 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30015166 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30015166 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30015166 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30015166 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30015166 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30015166 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30015166 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30015166 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30015166 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30015166 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30015166 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30015166 ID=SMED30015166|Name=SMED30015166|organism=Schmidtea mediterranea sexual|type=transcript|length=1080bpback to top protein sequence of SMED30015166-orf-1 >SMED30015166-orf-1 ID=SMED30015166-orf-1|Name=SMED30015166-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=111bp MREFIQNQTRIANHRKEIRTLEQDKQDQQNEIEKLREIIKGKETEIQEEGback to top protein sequence of SMED30015166-orf-2 >SMED30015166-orf-2 ID=SMED30015166-orf-2|Name=SMED30015166-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=100bp MMTQITDQARRWRIQYKILEDANAKQFEYIVKLVEHETVWQQTKIKEILLback to top Annotated Terms
The following terms have been associated with this transcript:
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